Effects of pulsed Nd:YAG laser kernel irradiation on maize (Zea mays L.): Insights into germination, gas exchange, photosynthetic pigments, and morphological modifications.

Energy has always been the most concerned topic worldwide due to its large consumption. Among various types of energies, light has amazing characteristics and have interesting effects on living organisms. Interest is increasing in the use of laser kernel treatment as an environment friendly physical technique for better results in agronomic crops, but the work is still in progress. The present study was conducted with the aim to examine the application of range of Neodymium-doped Yttrium Aluminum Garnet (Nd:YAG) pulsed laser exposures (200, 400, 600, 800, 1000, 1200, 1400 J/cm2 ) as pre-sowing kernel treatment on seedling survival rate, leaf photosynthetic activity in relation with photosynthetic pigments and visual morphological effects at seedling to maturity stage. Results showed that the low laser exposure (200, 400 and 600 J/cm2 ) improved the photosynthetic activity in parallel with improvement in chlorophyll a, chlorophyll b, total chlorophyll, carotenoids as well as morphological traits. Kernel treatments with higher laser fluences (800, 1000, 1200 and 1400 J/cm2 ) showed irregular responses in studied attributes examined at the individual plant level. At 800 and 1000 J/cm2 improvements were found in some plants but at higher doses clear negative impacts were recorded on studied attributes. In conclusion, the lower doses of Nd:YAG pulsed laser fluences are found beneficial for induction of improvement in maize plants for better growth but higher doses were found toxic ones. In future further studies are needed to check the impacts of low laser doses on yield related attributes under field conditions and the high doses might also be used to create variants with beneficial characteristics if possible.