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Journal of Photochemistry and Photobiology. B, Biology

Yang Liu, Shengzuo Fang, Wanxia Yang, Xulan Shang, Xiangxiang Fu
Understanding the responses of plant growth and secondary metabolites to differential light conditions is very important to optimize cultivation conditions of medicinal woody plants. As a highly valued and multiple function tree species, Cyclocarya paliurus is planted and managed for timber production and medical use. In this study, LED-based light including white light (WL), blue light (BL), red light (RL), and green light (GL) were used to affect leaf biomass production, flavonoid accumulation and related gene expression of one-year C...
January 11, 2018: Journal of Photochemistry and Photobiology. B, Biology
Jakub Guzikowski, Janusz Krzyścin, Agnieszka Czerwińska, Weronika Raszewska
Health-optimum-exposure index (HOEI) is proposed to assess if the prescribed amount of vitamin D3 (target value) could be synthesized in the human skin without erythema appearance. It is defined as the ratio between the vitamin D3 quantity received during the maximum allowed outdoor exposure without erythema risk and the target value. Sunbathing is safe for HOEI>1 and 1/HOEI represents a part of minimal erythema dose (MED) necessary to obtain the target value. We examine the following targets: a vitamin D3 quantity equivalent to 1000 IU vitamin D3 taken orally, and an optimal vitamin D3 quantity defined by Krzyścin et al...
January 11, 2018: Journal of Photochemistry and Photobiology. B, Biology
James V Gruber, Robert Holtz, Soo In Yang
In vitro human epidermal skin equivalent tissues (MatTek EpiDerm™) were employed to examine the influence of UVB radiation on various established inflammation markers in the presence of topically applied sunscreens. MatTek EpiDerm™ tissues were treated with 2.0mg/cm2 of an Experimental oleosome-based SPF 30 product or a commercial SPF 30 beach product. Tissues were irradiated with 300mJ/cm2 of UVB radiation. Inflammatory cytokines IL-1α, IL-6 and IL-8 as well as arachidonic acid cascade marker PGE2 were examined via ELISA-based antibody detection...
December 28, 2017: Journal of Photochemistry and Photobiology. B, Biology
Rafael Zarzuela, Manuel Jesús Luna, María Luisa Almoraima Gil, María Jesús Ortega, José María Palacios-Santander, Ignacio Naranjo-Rodríguez, Juan José Delgado, Laura María Cubillana-Aguilera
The objective of this work was to ascertain the nature of the components responsible for the reducing and stabilizing properties of Zostera noltii extracts that lead to gold nanoparticle formation using chemical techniques of analysis. In order to achieve this aim, we try the synthesis of AuNPs with three different extracts from plants collected in the Bay of Cádiz (Spain). The n-butanol extract produced the best results. Taking this into account, four fractions were isolated by Sephadex LH-20 column chromatography from this extract and we studied their activity...
December 28, 2017: Journal of Photochemistry and Photobiology. B, Biology
A Rajeswari, E Jackcina Stobel Christy, Anitha Pius
A study was carried out to investigate the degradation of organic contaminants (Congo red and Reactive yellow - 105) using cellulose acetate - polystyrene (CA-PS) membrane with and without ZnO impregnation. Scanning electron microscope (SEM), electron dispersive analysis of X-rays (EDAX), Fourier transform infrared spectrometer (FTIR), atomic force microscope (AFM) and thermogravimeric analysis (TG-DTA) analysis were carried out to characterize bare and ZnO impregnated CA-PS membranes. Membrane efficiency was also tested for pure water flux and antifouling performance...
December 27, 2017: Journal of Photochemistry and Photobiology. B, Biology
Luyao Lu, Lingyan Shi, Jeff Secor, Robert Alfano
This study aimed to use self-absorption correction to determine the Raman enhancement of β-carotene. The Raman spectra of β-carotene solutions were measured using 488nm, 514nm, 532nm and 633nm laser beams, which exhibited significant resonance Raman (RR) enhancement when the laser energy approaches the electronic transition energy from S0 to S2 state. The Raman intensity and the actual resonance Raman gain without self-absorption from S2 state by β-carotene were also obtained to evaluate the effect of self-absorption on RR scattering...
December 22, 2017: Journal of Photochemistry and Photobiology. B, Biology
Gwendal Josse, Thierry Douki, Jimmy Le Digabel, Eleonore Gravier, Emmanuel Questel
The formation of DNA photoproducts caused by solar UVR exposure needs to be investigated in-vivo and in particular in order to assess sunscreens' level of protection against solar genotoxicity. The study's purposes were: i) to evaluate if the roof of suction blisters is an appropriate sampling method for measuring photoproducts, and ii) to measure in-vivo sunscreen protection against cyclobutane pyrimidine dimers. Skin areas on the interior forearms of eight healthy volunteers were exposed in-vivo to 2 MED of simulated solar radiation (SSR) and to 15 MED on a sunscreen protected area...
December 22, 2017: Journal of Photochemistry and Photobiology. B, Biology
Sabera Millan, Lakkoji Satish, Krishnendu Bera, Monidipa Konar, Harekrushna Sahoo
In this present work, a detailed investigation of the effect of an anticancer drug, 5-Fluorouracil (5-FU), on conformation, stability and activity of lysozyme (Lyz) was reported. The interaction between Lyz and 5-FU was reflected in terms of intrinsic fluorescence quenching and change in secondary structure of Lyz. The mode of quenching mechanism involved was evaluated by the steady-state and time-resolved fluorescence measurements. Synchronous and Circular Dichroism (CD) results revealed the conformational changes induced in Lyz upon complexation with 5-FU...
December 21, 2017: Journal of Photochemistry and Photobiology. B, Biology
Aruppillai Suthaparan, Ranjana Pathak, Knut Asbjørn Solhaug, Hans Ragnar Gislerød
Controlled environment chamber experiments at Petri dish level were conducted to examine the wavelength and dose dependent efficacy of ultraviolet (UV) radiation, the recovery action potential of optical radiation applied concomitantly/subsequently to effective UV treatment, and the lapse time between UV treatment and subsequent exposure to recovery wavelength on germination efficiency of Oidium neolycopersici conidia. Conidia of eight- to nine-day-old colonies were dusted on water agar surface in Petri dishes and exposed to UV treatments (without lid)...
December 18, 2017: Journal of Photochemistry and Photobiology. B, Biology
Alena I Gavrina, Marina V Shirmanova, Nadeshda A Aksenova, Diana V Yuzhakova, Ludmila B Snopova, Anna B Solovieva, Peter S Тimashev, Varvara V Dudenkova, Elena V Zagaynova
The use of polymeric carriers to deliver hydrophobic photosensitizers has been widely discussed as a way to improve both fluorescence diagnostic and photodynamic therapy (PDT) of cancers; however, the photophysical and pharmacokinetic parameters, as well as the PDT activity, of such modifications have, until now, only been poorly investigated. The purpose of the present study was to explore the efficacy of PDT with the formulation of the photosensitizer chlorin e6 (Ce6) in combination with polyvinyl alcohol (PVA) in comparison with Ce6 alone and with the clinical drug, Photodithazine in a mouse tumor model...
December 15, 2017: Journal of Photochemistry and Photobiology. B, Biology
Jyoti Singh, Ajeet K Srivastva, Payal Mandal, Sonam Chandra, Divya Dubey, Ashish Dwivedi, Deepti Chopra, Anurag Tripathi, Ratan Singh Ray
Pefloxacin (PFLX) is an antibiotic, which shows broad spectrum antimicrobial activities. It is an important derivative of fluoroquinolones (FLQs) group. Ultraviolet radiation (200-400nm) causes major problem for living being which comes at the earth surface naturally through sunlight and increasing regularly due to ozone depletion. PFLX was photodegraded in 5h and forms photoproduct under UVA exposure. At the non photocytotoxic dose PFLX, shows reduced phagocytosis activity, NO (nitric oxide) production, large vacuole formation and down regulated IL-6, TNF-α and IL-1 in BALB/c macrophages at both genes and proteins levels...
December 15, 2017: Journal of Photochemistry and Photobiology. B, Biology
Tobias Bornhütter, Nedaa Shamali, Irena Saltsman, Atif Mahammed, Zeev Gross, Georg Däschlein, Beate Röder
A treatment of onychomycosis using the photodynamic effect would be a favorable alternative to currently used antimycotic drugs. This study should be considered as a first step towards development and control of an efficient photodynamic inactivation of onychomycosis causative pathogens. Here, we evaluate the usage of time-resolved 2D singlet oxygen luminescence detection in combination with 2D fluorescence scanning as a tool to understand the behavior of the photosensitizer when applied to fungi on Petri dishes...
December 14, 2017: Journal of Photochemistry and Photobiology. B, Biology
Ting Zhang, Alok Sharma, Yulong Li, Yaoyao Zhou, Xianting Ding
Excessive exposure to ultraviolet (UV) B radiation may lead to skin damage, photosensitivity, or even tumorigenesis via induction of oxidative stress. Naturally derived antioxidants could play significant roles in cancer therapy due to their multi-targeted actions and lack of substantial toxicity. Drug combinations target at diverse pathway of cells and make cells export meticulous biological outcomes through the multifaceted signaling network. The UVB protective effects of combinations of naturally derived antioxidants- curcumin, resveratrol, proanthocyanidins, baicalein, and beta-nicotinamide adenine dinucleotide (NADH) were investigated...
December 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
Myalowenkosi I Sabela, Talent Makhanya, Suvardhan Kanchi, Mohd Shahbaaz, Danish Idress, Krishna Bisetty
Plant extracts continue gaining significant prominence in green synthesis of silver nanoparticles (AgNPs), due to their potential applications in nano-medicine and material engineering. This work reports on green synthesis of silver nanoparticles (AgNPs) from aqueous extracts of Iboza Riparia leaf and Ilex Mitis root bark with diterpenes (DTPs) and saponins (SPNs) as major components. After TEM, DLS, TGA/DSC, ATR, XRD and UV-Vis characterization, the relevant cytotoxicity studies were conducted with the MTT assay on human embryonic kidney cells (HEK293T) followed by antioxidant activity with ABTS...
December 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
Parammal Faseela, Jos T Puthur
High light and ultraviolet-B radiation (UV-B) are generally considered to have negative impact on photosynthesis and plant growth. The present study evaluates the tolerance potential of three cultivars of Oryza sativa L. (Kanchana, Mattatriveni and Harsha) seedlings towards high light and UV-B stress on the basis of photosynthetic pigment degradation, chlorophyll a fluorescence parameters and rate of lipid peroxidation, expressed by malondialdehyde content. Surprisingly, it was revealed that Kanchana was the most sensitive cultivar towards high light and at the same time it was the most tolerant cultivar towards UV-B stress...
December 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
Saswata Chatterjee, Yunhee Rhee, Phil-Sang Chung, Rui-Feng Ge, Jin-Chul Ahn
Sulforaphene (SFE), a natural isothiocyanate from cruciferous vegetables has shown a potential anticancer effect against cervical and lung cancer. Palliative treatments like photodynamic therapy (PDT) are being implemented for a long time however, the results are still not promising in case of aggressive cancers like anaplastic thyroid cancer. The objective of this work is to establish an alternative method with the combination of photofrin-PDT and sulforaphene, a natural isothiocyanate from cruciferous vegetables, against human anaplastic thyroid cancer to enhance the efficacy of PDT...
December 11, 2017: Journal of Photochemistry and Photobiology. B, Biology
Jitka Vostálová, Martin Cukr, Bohumil Zálešák, Radka Lichnovská, Jitka Ulrichová, Alena Rajnochová Svobodová
Skin explants are a suitable model which can replace dermatological experiments on animals or human volunteers. In this study, we searched for a fast, cheap and reproducible method for screening skin explant viability after treatment with UVA radiation or/and chemical agents. We compared frequently used methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), neutral red (NR) and lactate dehydrogenase (LDH) activity assay with a rarely used 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) assay for the evaluation of UVA radiation and/or chlorpromazine and 8-methoxypsoralen effect as model agents...
December 9, 2017: Journal of Photochemistry and Photobiology. B, Biology
Aleksandra Krzywon, Maria Widel, Krzysztof Fujarewicz, Magdalena Skonieczna, Joanna Rzeszowska-Wolny
UVA radiation, which accounts for about 95% of the solar spectrum, contributes to and may be the etiological factor of skin cancers of which malignant melanoma is the most aggressive. UVA causes oxidative stress in various types of cells in the skin, keratinocyte, melanocytes, and fibroblasts, which is responsible for its cytotoxic effect. Here we used a transwell system to explore how the responses of melanoma cells to a low dose of UVA (20kJ/m2, ~10% of the minimal erythema dose) are influenced by neighboring co-cultured melanoma cells or fibroblasts...
December 9, 2017: Journal of Photochemistry and Photobiology. B, Biology
Maryam Hosseini-Zori
The aim of this work was synthesis and investigation of various properties of Co-doped titanium dioxide nanostructures. However, dopant has no effect on XRD pattern of the host but it can influence on the various characteristics of host such as optical and electrical properties. The results of optical properties showed that absorption energy of TiO2 decreases in presence of cobalt as dopant. Red-shift in absorption spectrum that may be due to the excitation of 3d electrons of Co ions to the conduction band of TiO2 can be considered as a strong evidence to confirm the presence of Co as dopant in TiO2 lattice...
December 8, 2017: Journal of Photochemistry and Photobiology. B, Biology
Yogesh Gawale, Nagaiyan Sekar
Density Functional Theory and Time Dependent Density Functional Theory computations reveal experimental trends accurately and provide an insight into the origin of red shifted optical spectra, large Stokes shift, nonlinear optical responses and quantitative description of the singlet-triplet energy gap of recently synthesized highly fluorescent membrane permeable benz[c,d]indole N-heteroarene BF2 rigidified BBN and BBC dyes. Our results benchmark the importance of the DFT functionals and polarization functions using the accurate basis set for the calculations of excitation energies, band gaps, dipole moment, non-linear optical properties and geometrical parameters...
December 7, 2017: Journal of Photochemistry and Photobiology. B, Biology
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