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Journal Article
Research Support, Non-U.S. Gov't
The effect of enzyme replacement on red cell adenine deoxyribonucleotides in adenosine deaminase-deficient erythrocytes of the opossum, Didelphis virginiana.
1. Polyethyleneglycol-modified bovine adenosine deaminase was administered (10-20 U/kg/week) intramuscularly to two opossums for 15 weeks and changes in red cell adenine ribo- and deoxyribonucleotides quantitated by HPLC. 2. Only a moderate decline of erythrocyte dAXP was observed at the end of the study when compared to results of enzyme replacement seen in human adenosine deaminase deficient patients. 3. Opossum red cells salvage substantial amounts of deoxyadenosine provided in physiologic (50 nM) concentration from plasma having either low or high adenosine deaminase activity.
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