An in vitro evaluation of the effects of fluoride, CPP-ACP, or resin infiltration on discoloration caused by pediatric supplements.

OBJECTIVES: White spot lesions (WSLs) are more susceptible to staining agents due to their porous structure. This study examines the impact of WSL treatments on discoloration caused by pediatric supplements.

METHOD AND MATERIALS: Three treatments (fluoride, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), resin infiltration (RI)), a control, and their respective syrup-based subgroups (iron and black elderberry syrups) were established, each with 8 teeth. Artificial WSLs were induced, and weekly applications of fluoride varnish, daily applications of CPP-ACP paste and a single resin infiltration procedure were performed on the WSLs within the treatment groups over 4 weeks. Simultaneously, samples were exposed daily to iron or black elderberry syrups. Spectrophotometer measurements were taken at baseline, after demineralization (T0), and after one (T1), two (T2), and four weeks (T4). ΔE00 values were calculated. Statistical analysis was conducted using a three-way mixed-design ANOVA, with the significance level set at P = .05 Results: At T4, ΔE00 values from all groups exceeded clinical acceptability limit of 1.8. At T2 and T4, the ΔE00 values obtained from the black elderberry syrup subgroups were significantly higher (P < .001). At T4, the highest ΔE00 values were seen in the CPP-ACP groups (P < .001). The lowest ΔE00 values at T2 and T4 were observed in the RI groups (P < .05).

CONCLUSIONS: Supplements containing ferrous sulfate and black elderberry extract caused color changes in WSLs that exceeded the clinical acceptability limit. Resin infiltration of WSLs provides advantages over remineralization treatments, particularly in minimizing discoloration induced by pediatric supplements.