We have located links that may give you full text access.
Advancing perfusion models: Dual-vessel ex vivo rat liver perfusion based on a clinical setup.
Tissue Engineering. Part A 2023 July 28
Normothermic ex vivo liver machine perfusion (NEVLP) has been developed to address the increasing organ shortage in liver transplantation, potentially through pharmacological conditioning of grafts originating from extended criteria donors. To establish new therapeutic concepts, simple and standardized animal models are needed, that simulate clinical NEVLP as closely as possible. Liver grafts from 36 Sprague-Dawley rats were perfused for 6 h in a dual-vessel NEVLP system using varying doses of the clinically used vasodilator epoprostenol, with and without the addition of the Kupffer cell inhibitor glycine. Cell culture medium supplemented with rat plasma and erythrocytes was compared to Steen-based perfusion solution, which is used in clinical NEVLP. Perfusion pressures and bile production were recorded, and perfusate was analyzed for transaminase secretion. Tissue samples were assessed histologically, and tissue levels of cytokines and 8-Isoprostane were measured. Increasing levels of epoprostenol and the addition of glycine resulted in a stepwise decrease of transaminase secretion and improved bile production. Steen significantly decreased transaminase release as well as IL-1β production. Liver grafts perfused with the optimised Steen-based protocol exhibited lowest levels of oxidative stress with best-preserved liver integrity. In conclusion, high doses of epoprostenol seem to ameliorate liver function and prevent cellular damage beyond the vasodilatory effect, with glycine acting synergistically. The anti-inflammatory and anti-oxidative properties of Steen further optimise perfusion conditions. Our rodent NEVLP system may be used to rapidly test new agents for pharmacologic conditioning of livers and to translate these findings from bench-to-bedside.
Full text links
Related Resources
Trending Papers
Consensus Statement on Vitamin D Status Assessment and Supplementation: Whys, Whens, and Hows.Endocrine Reviews 2024 April 28
The Tricuspid Valve: A Review of Pathology, Imaging, and Current Treatment Options: A Scientific Statement From the American Heart Association.Circulation 2024 April 26
Intravenous infusion of dexmedetomidine during the surgery to prevent postoperative delirium and postoperative cognitive dysfunction undergoing non-cardiac surgery: a meta-analysis of randomized controlled trials.European Journal of Medical Research 2024 April 19
Interstitial Lung Disease: A Review.JAMA 2024 April 23
Management of Diverticulitis: A Review.JAMA Surgery 2024 April 18
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app