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Tissue Engineering. Part A

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https://www.readbyqxmd.com/read/27829314/bone-morphogenetic-protein-2-but-not-mesenchymal-stromal-cells-exert-regenerative-effects-on-canine-and-human-nucleus-pulposus-cells
#1
Frances C Bach, Alberto Miranda-Bedate, Ferdi W M van Heel, Frank M Riemers, Margot C M E Müller, Laura B Creemers, Keita Ito, Karin Benz, Björn P Meij, Marianna A Tryfonidou
Chronic back pain is related to intervertebral disc (IVD) degeneration and dogs are employed as animal models to develop growth factor- and cell-based regenerative treatments. In this respect, the differential effects of transforming growth factor beta-1 (TGF-β1) and bone morphogenetic protein-2 (BMP2) on canine and human chondrocyte-like cells (CLCs) derived from the nucleus pulposus of degenerated IVDs were studied. Human and canine CLCs were cultured in 3D microaggregates in basal culture medium supplemented with/without TGF-β1 (10 ng/mL) or BMP2 (100 or 250 ng/mL)...
December 12, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27824280/the-combined-effect-of-substrate-stiffness-and-surface-topography-on-chondrogenic-differentiation-of-mesenchymal-stem-cells
#2
Yingnan Wu, Zheng Yang, Jaslyn Bee Khuan Law, Ai Yu He, Azlina A Abbas, Vinitha Denslin, Tunku Kamarul, James Hp Hui, Eng Hin Lee
Stem cell differentiation is guided by contact with the physical microenvironment, influence by both topography and mechanical properties of the matrix. In this study, the combined effect of substratum nano-topography and mechanical stiffness in directing mesenchymal stem cell (MSC) chondrogenesis was investigated. Three polyesters of varying stiffness were thermally imprinted to create nano-grating or pillar patterns of the same dimension. The surface of the nano-patterned substrate was coated with chondroitin sulfate (CS) to provide an even surface chemistry, with cell-adhesive and chondro-inductive properties, across all polymeric substrates...
December 7, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27923328/modeling-stroma-induced-drug-resistance-in-a-tissue-engineered-tumor-model-of-ewing-sarcoma
#3
Marco Santoro, Brian A Menegaz, Salah-Eddine Lamhamedi-Cherradi, Eric R Molina, Danielle Wu, Waldemar Priebe, Joseph A Ludwig, Antonios G Mikos
Three-dimensional (3D) tumor models are gaining traction in the research community given their capacity to mimic aspects of the tumor microenvironment absent in monolayer systems. In particular, the ability to spatiotemporally control cell placement within ex vivo 3D systems has enabled the study of tumor-stroma interactions. Furthermore, by regulating biomechanical stimuli, one can reveal how biophysical cues affect stromal cell phenotype and how their phenotype impacts tumor drug sensitivity. Both tumor architecture and shear force have profound effects on Ewing sarcoma (ES) cell behavior and are known to elicit ligand-mediated activation of the insulin-like growth factor-1 receptor (IGF-1R), thereby mediating resistance of ES cells to IGF-1R inhibitors...
December 6, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27923325/a-gingiva-derived-mesenchymal-stem-cell-laden-porcine-small-intestinal-submucosa-extracellular-matrix-construct-promotes-myomucosal-regeneration-of-the-tongue
#4
Qilin Xu, Rabie M Shanti, Qunzhou Zhang, Steven B Cannady, Bert W O'Malley, Anh D Le
In the oral cavity, the tongue is the anatomic subsite most commonly involved by invasive squamous cell carcinoma. Current treatment protocols often require significant tissue resection to achieve adequate negative margins and optimal local tumor control. Reconstruction of the tongue while preserving and/or restoring its critical vocal, chewing, and swallowing functions remains one of the major challenges in head and neck oncologic surgery. We investigated the in vitro feasibility of fabricating a novel combinatorial construct using porcine small intestinal submucosa extracellular matrix (SIS-ECM) and human gingival mesenchymal stem cells (GMSCs) as a GMSC/SIS-ECM tissue graft for the tongue reconstruction...
December 6, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27784202/cellular-self-assembly-with-microsphere-incorporation-for-growth-factor-delivery-within-engineered-vascular-tissue-rings
#5
Hannah A Strobel, Anna D Dikina, Karen Levi, Loran D Solorio, Eben Alsberg, Marsha W Rolle
Cellular self-assembly has been used to generate living tissue constructs as an alternative to seeding cells on or within exogenous scaffold materials. However, high cell and extracellular matrix density in self-assembled constructs may impede diffusion of growth factors during engineered tissue culture. In the present study, we assessed the feasibility of incorporating gelatin microspheres within vascular tissue rings during cellular self-assembly to achieve growth factor delivery. To assess microsphere incorporation and distribution within vascular tissue rings, gelatin microspheres were mixed with a suspension of human smooth muscle cells (SMCs) at 0, 0...
December 6, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27903131/hypoxic-expansion-of-human-mesenchymal-stem-cells-enhances-3d-maturation-of-tissue-engineered-intervertebral-discs
#6
Katherine Deline Hudson, Lawrence J Bonassar
Culture of three dimensional (3D) constructs in hypoxic conditions (1-5% O2) has been shown to increase production of extracellular matrix (ECM) components in primary intervertebral disc (IVD) cells, and drive chondrogenesis of human mesenchymal stem cells (MSCs). Growing evidence that suggests that two dimensional (2D) expansion under hypoxic conditions may have an even greater influence on chondrogenesis in MSCs. This study aims to determine the effects of hypoxia during 2D expansion and subsequent 3D culture on the in vitro maturation of tissue engineering IVDs (TE-IVDs) made with human MSCs, using a previously developed TE-IVD system...
November 30, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27899064/gdf5-mediated-enhancement-of-chondrocyte-phenotype-is-inhibited-by-heparin-implication-for-the-use-of-heparin-in-the-clinic-and-in-tissue-engineering-applications
#7
Bethanie Imogen Ayerst, Raymond A A Smith, Victor Nurcombe, Anthony J Day, Catherine L R Merry, Simon Cool
The highly sulfated glycosaminoglycan (GAG) heparin is widely used in the clinic as an anticoagulant, and researchers are now using it to enhance stem cell expansion/ differentiation protocols, as well as to improve the delivery of growth factors for tissue engineering strategies. Growth differentiation factor 5 (GDF5) belongs to the bone morphogenetic protein family of proteins and is vital for skeletal formation, however, its interaction with heparin and heparan sulfate (HS) has not been studied. We identify GDF5 as a novel heparin/ HS-binding protein, and show that HS proteoglycans are vital in localizing GDF5 to the cell surface...
November 29, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27771997/enhanced-mandibular-bone-repair-by-combined-treatment-of-bone-morphogenetic-protein-2-and-small-molecule-phenamil
#8
Jiabing Fan, Mian Guo, Choong Sung Im, Joan Pi-Anfruns, Zhong-Kai Cui, Soyon Kim, Benjamin M Wu, Tara L Aghaloo, Min Lee
Growth factor-based therapeutics using bone morphogenetic protein 2 (BMP-2) presents a promising strategy to reconstruct craniofacial bone defects such as mandible. However, clinical applications require supraphysiological BMP doses that often increase inappropriate adipogenesis, resulting in well-documented, cyst-like bone formation. Here we reported a novel complementary strategy to enhance osteogenesis and mandibular bone repair by using small-molecule phenamil that has been shown to be a strong activator of BMP signaling...
November 28, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27796164/comparison-of-perfluorodecalin-and-hemoxcell-as-oxygen-carriers-for-islet-oxygenation-in-an-in-vitro-model-of-encapsulation
#9
Aida Rodriguez-Brotons, William Bietiger, Claude Peronet, Allan Langlois, Jordan Magisson, Carole Mura, Cynthia Sookhareea, Valerie Polard, Nathalie Jeandidier, Franck Zal, Michel Pinget, Séverine Sigrist, Elisa Maillard
Transplantation of encapsulated islets in a bioartificial pancreas is a promising alternative to free islet cell therapy to avoid immunosuppressive regimens. However, hypoxia, which can induce a rapid loss of islets, is a major limiting factor. The efficiency of oxygen delivery in an in vitro model of bioartificial pancreas involving hypoxia and confined conditions has never been investigated. Oxygen carriers such as perfluorocarbons and hemoglobin might improve oxygenation. To verify this hypothesis, this study aimed to identify the best candidate of perfluorodecalin (PFD) or HEMOXCell(®) to reduce cellular hypoxia in a bioartificial pancreas in an in vitro model of encapsulation ex vivo...
November 24, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27875939/injectable-shape-memorizing-3d-hyaluronic-acid-cryogels-3d-ma-ha-for-skin-sculpting-and-soft-tissue-reconstruction
#10
Liying Cheng, Kai Ji, Ting-Yu Shih, Anthony Haddad, Giorgio Giatsidis, David J Mooney, Dennis P Orgill, Christoph S Nabzdyk
Introduction Hyaluronic acid based (HA) fillers are used for various cosmetic procedures. However, due to filler migration and degradation, reinjections of the fillers are often required. Methacrylated HA (MA-HA) can be made into injectable, shape-memorizing fillers (3D MA-HA) aimed to address this issue. In this study shape retention, firmness and biocompatibility of 3D MA-HA injected subcutaneously in mice were evaluated. Methods Fifteen mice, each receiving two subcutaneous injections in their back, were divided into 4 groups receiving HA, MA-HA, 3D MA-HA or saline, respectively...
November 22, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27762658/effects-of-stem-cell-factor-on-cell-homing-during-functional-pulp-regeneration-in-human-immature-teeth
#11
Nisarat Ruangsawasdi, Matthias Zehnder, Raphael Patcas, Chafik Ghayor, Barbara Siegenthaler, Bebeka Gjoksi, Franz E Weber
Conventional root canal treatment in immature permanent teeth can lead to early tooth loss in children because root formation is discontinued. We investigated whether the stem cell factor (SCF) could facilitate cell homing in the pulpless immature root canal and promote regeneration of a functional pulp. In vitro, human mesenchymal stem cells (hMSCs) were exposed to SCF at various concentrations for assessing cell migration, proliferation, and differentiation toward odonto/osteoblasts by 3D-chemotaxis slides, WST-1 assay, and alkaline phosphatase activity, respectively...
November 22, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27712409/anisotropic-shape-memory-alginate-scaffolds-functionalized-with-either-type-i-or-type-ii-collagen-for-cartilage-tissue-engineering
#12
Henrique V Almeida, Binulal N Sathy, Ivan Dudurych, Conor T Buckley, Fergal J O'Brien, Daniel J Kelly
Regenerating articular cartilage and fibrocartilaginous tissue such as the meniscus is still a challenge in orthopedic medicine. While a range of different scaffolds have been developed for joint repair, none have facilitated the development of a tissue that mimics the complexity of soft tissues such as articular cartilage. Furthermore, many of these scaffolds are not designed to function in mechanically challenging joint environments. The overall goal of this study was to develop a porous, biomimetic, shape-memory alginate scaffold for directing cartilage regeneration...
November 22, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27869543/direct-bonding-of-chitosan-biomaterials-to-tissues-using-transglutaminase-for-surgical-repair-or-device-implantation
#13
Javier Fernandez, Suneil Seetharam, Christopher Ding, Edward Doherty, Donald Ingber
Natural biomaterials, such as chitosan and collagen, are useful for biomedical applications because they are biocompatible, mechanically robust and biodegradable, but it is difficult to rapidly and tightly bond them to living tissues. Here, we demonstrate that the microbial enzyme transglutaminase (mTG) can be used to rapidly (< 5 min) bond chitosan and collagen biomaterials to the surfaces of hepatic, cardiac and dermal tissues, as well as to functionalized polydimethylsiloxane (PDMS) materials that are used in medical products...
November 21, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27736317/a-three-dimensional-chondrocyte-macrophage-coculture-system-to-probe-inflammation-in-experimental-osteoarthritis
#14
Satyavrata Samavedi, Patricia Diaz-Rodriguez, Joshua D Erndt-Marino, Mariah S Hahn
The goal of the present study was to develop a fully three-dimensional (3D) coculture system that would allow for systematic evaluation of the interplay between activated macrophages (AMs) and chondrocytes in osteoarthritic disease progression and treatment. Toward this end, our coculture system was first validated against existing in vitro osteoarthritis models, which have generally cultured healthy normal chondrocytes (NCs)-in two-dimensional (2D) or 3D-with proinflammatory AMs in 2D. In this work, NCs and AMs were both encapsulated within poly(ethylene glycol) diacrylate hydrogels to mimic the native 3D environments of both cell types within the osteoarthritic joint...
November 18, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27707109/efficacy-of-particulate-amniotic-membrane-and-umbilical-cord-tissues-in-attenuating-cartilage-destruction-in-an-osteoarthritis-model
#15
Andrew L Raines, Mei-Shu Shih, Lorraine Chua, Chen-Wei Su, Scheffer C G Tseng, Julie O'Connell
Osteoarthritis (OA) is a progressive degenerative joint disease, and to date, no disease-modifying OA drug exists. Amniotic membrane and umbilical cord products have been used clinically in several diseases due to their anti-inflammatory and antiscarring properties. In the present study, we sought to evaluate whether a particulate amniotic membrane and umbilical cord (AM/UC) matrix could aid in attenuating disease progression. Lewis rats underwent medial meniscus transection (MMT) to induce OA. Two weeks after surgery, animals received intra-articular injections (50 μL) of either 50 or 100 μg/μL particulate AM/UC or saline control and were subsequently euthanized 1 or 4 weeks later...
November 18, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27835935/a-new-vertebral-body-replacement-strategy-using-expandable-polymeric-cages
#16
Xifeng Liu, Alex Paulsen, Hugo Giambini, Ji Guo, Alan Miller, Po-Chun Lin, Michael J Yaszemski, Lichun Lu
We have developed a novel polymeric expandable cage that can be delivered via a posterior-only surgical approach for the treatment of non-contained vertebral defects. This approach is less invasive than an anterior-only or combined approach and much more cost effective than currently used expandable metal cages. The polymeric expandable cage is composed of oligo poly(ethylene glycol) fumarate (OPF), a hydrogel that has been previously shown to have excellent nerve and bone tissue biocompatibility. OPF hydrogel cages can expand to twice their original diameter and length within a surgical time frame following hydration...
November 11, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27824284/formation-of-hyaline-cartilage-tissue-by-passaged-human-osteoarthritic-chondrocytes
#17
Vanessa Juliana Bianchi, Joanna F Weber, Stephen D Waldman, David Backstein, Rita Kandel
Objective When serially passaged in standard monolayer culture to expand cell number, articular chondrocytes lose their phenotype. This results in the formation of fibrocartilage when they are used clinically, thus limiting their use for cartilage repair therapies. Identifying a way to redifferentiate these cells in vitro is critical if they are to be used successfully. Transforming growth factor beta (TGFβ) family members are known to be crucial for regulating differentiation of fetal limb mesenchymal cells and mesenchymal stromal cells to chondrocytes...
November 8, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27814669/laminin-alginate-beads-as-preadipocyte-carriers-to-enhance-adipogenesis-in-vitro-and-in-vivo
#18
Yu-Sheng Hsueh, Yo-Shen Chen, Hao-Chih Tai, Ondrej Mestak, Sung-Chuan Chao, Yen-Yu Chen, Ying Shih, Jung-Feng Lin, Ming-Jium Shieh, Feng-Huei Lin
The use of autologous fat grafting in breast reconstruction still requires optimization. Fat survival and calcification are the main issues that affect the outcomes of the procedure. In this study, a cell-based therapy utilizing laminin-alginate beads (LABs) as carriers was proposed to promote cell survival and adipogenesis by providing short-term physical support and facilitate nutrient diffusion of the implants. Laminin-modified alginate beads were fabricated by immobilizing laminin onto ring-opened alginate, used to encapsulate 3T3-L1 preadipocytes, and evaluated in vitro and in vivo...
November 4, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27809678/tendon-derived-extracellular-matrix-enhances-tgf-%C3%AE-3-induced-tenogenic-differentiation-of-human-adipose-derived-stem-cells
#19
Guang Yang, Benjamin B Rothrauff, Hang Lin, Shuting Yu, Rocky Tuan
Because of the limited and unsatisfactory outcomes of clinical tendon repair, tissue engineering approaches using adult mesenchymal stem cells (MSCs) are being considered as a promising alternative strategy to heal tendon injuries. Successful and functional tendon tissue engineering depends on harnessing the biochemical cues presented by the native tendon extracellular matrix (ECM) and the embedded tissue-specific bio-factors. In this study, we have prepared and characterized the biological activities of a soluble extract of decellularized tendon ECM (tECM) on adult adipose derived stem cells (ASCs), on the basis of histological, biochemical, and gene expression analyses...
November 3, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27806678/decellularisation-and-characterisation-of-porcine-superflexor-tendon-a-potential-anterior-cruciate-ligament-replacement
#20
Gemma Jones, Anthony Herbert, Helen Berry, Jennifer Helen Edwards, John Fisher, Eileen Ingham
The porcine superflexor tendon (SFT) was identified as having appropriate structure and properties for development of a decellularised device for use in ACL reconstruction. SFTs were decellularised using a combination of freeze thaw and washes in hypotonic buffer and 0.1% (w/v) SDS in hypotonic buffer plus proteinase inhibitors followed by nuclease treatment and sterilisation using peracetic acid. The decellularised biological scaffold was devoid of cells and cell remnants and contained only 13 ng.mg<sup>-1</sup> (dry weight) residual total DNA...
November 2, 2016: Tissue Engineering. Part A
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