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Tissue Engineering. Part A

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https://www.readbyqxmd.com/read/28081675/3d-printed-porous-poly-propylene-fumarate-scaffolds-with-delayed-rhbmp-2-release-for-acl-graft-fixation
#1
Joshua Parry, Maurits Geert Laurent Olthof, Kristen L Shogren, Mahrokh Dadsetan, Andre J van Wijnen, Michael J Yaszemski, Sanjeev Kakar
Anterior cruciate ligament (ACL) ruptures reconstructed with tendon grafts are commonly fixed with bioabsorbable implants, which are frequently complicated by incomplete bone filling upon degradation. Bone regeneration after ACL reconstruction could be enhanced by utilizing tissue engineering techniques and three-dimensional (3D) printing to create a porous bioabsorbable scaffold with delayed delivery of recombinant-human bone morphogenetic protein 2 (rhBMP-2). The first aim of this study was to design a 3D PPF porous scaffold that maintained suitable pull-out strength for future testing in a rabbit ACL reconstruction model...
January 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27998243/effects-of-local-antibiotic-delivery-from-porous-space-maintainers-on-infection-clearance-and-induction-of-an-osteogenic-membrane-in-an-infected-bone-defect
#2
Sarita R Shah, Brandon T Smith, Alexander M Tatara, Eric R Molina, Esther J Lee, Trenton C Piepergerdes, Brent A Uhrig, Robert E Guldberg, George N Bennett, Joseph C Wenke, Antonios G Mikos
Reconstruction of large bone defects can be complicated by the presence of both infection and local antibiotic administration. This can be addressed through a two-stage reconstructive approach, called the Masquelet technique, that involves the generation of an induced osteogenic membrane over a temporary poly(methyl methacrylate) (PMMA) space maintainer, followed by definitive reconstruction after the induced membrane is formed. Given that infection and antibiotic delivery each have independent effects on local tissue response, the objective of this study is to evaluate the interaction between local clindamycin release and bacterial contamination with regards to infection prevention and the restoration of pro-osteogenic gene expression in the induced membrane...
January 11, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28073315/metabolically-active-3-dimensional-brown-adipose-tissue-engineered-from-white-adipose-derived-stem-cells
#3
Jessica P Yang, Amy Elizabeth Anderson, Annemarie McCartney, Xavier Ory, Garret Ma, Elisa Pappalardo, Joel Bader, Jennifer Elisseeff
Brown adipose tissue (BAT) has a unique capacity to expend calories by decoupling energy expenditure from ATP production, therefore BAT could realize therapeutic potential to treat metabolic diseases such as obesity and type 2 diabetes. Recent studies have investigated markers and function of native BAT, however, successful therapies will rely on methods that supplement the small existing pool of brown adipocytes in adult humans. In this study, we engineered BAT from both human and rat adipose precursors and determined whether these ex vivo constructs could mimic in vivo tissue form and metabolic function...
January 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28073305/human-amniotic-mesenchymal-stromal-cells-as-favourable-source-for-cartilage-repair
#4
Emma Muinos-López, Tamara Hermida-Gómez, Isaac Fuentes-Boquete, Francisco Javier de Toro-Santos, Francisco J Blanco, Silvia María Díaz-Prado
INTRODUCTION: Localized trauma-derived breakdown of the hyaline articular cartilage may progress towards osteoarthritis, a degenerative condition characterized by total loss of articular cartilage and joint function. Tissue engineering technologies encompass several promising approaches with high therapeutic potential for the treatment of these focal defects. However, most of the research in tissue engineering is focused on potential materials and structural cues, while little attention is directed to the most appropriate source of cells endowing these materials...
January 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28071988/braided-and-stacked-electrospun-nanofibrous-scaffolds-for-tendon-and-ligament-tissue-engineering
#5
Benjamin B Rothrauff, Brian B Lauro, Guang Yang, Richard E Debski, Volker Musahl, Rocky Tuan
Tendon and ligament injuries are a persistent orthopaedic challenge given their poor innate healing capacity. Non-woven electrospun nanofibrous scaffolds composed of polyesters have been used to mimic the mechanics and topographical cues of native tendons and ligaments. However, non-woven nanofibers have several limitations that prevent broader clinical application, including poor cell infiltration as well as tensile and suture-retention strengths that are inferior to native tissues. In this study, multilayered scaffolds of aligned electrospun nanofibers of two designs - stacked or braided - were fabricated...
January 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28071973/collagen-cross-linking-biophysical-biochemical-and-biological-response-analysis
#6
L Delgado, Kieran Fuller, Dimitrios I Zeugolis
Extracted forms of collagen are subjected to chemical cross-linking to enhance their stability. However, traditional cross-linking approaches are associated with toxicity and inflammation. This work investigates the stabilisation capacity, cytotoxicity and inflammatory response of collagen scaffolds cross-linked with glutaraldehyde, carbodiimide, 4-arm polyethylene glycol succinimidyl glutarate, genipin and oleuropein. Although all cross-linking methods reduced free amine groups, variable data were obtained with respect to denaturation temperature, resistance to collagenase digestion and mechanical properties...
January 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27809678/tendon-derived-extracellular-matrix-enhances-transforming-growth-factor-%C3%AE-3-induced-tenogenic-differentiation-of-human-adipose-derived-stem-cells
#7
Guang Yang, Benjamin B Rothrauff, Hang Lin, Shuting Yu, Rocky S Tuan
Because of the limited and unsatisfactory outcomes of clinical tendon repair, tissue engineering approaches using adult mesenchymal stem cells are being considered a promising alternative strategy to heal tendon injuries. Successful and functional tendon tissue engineering depends on harnessing the biochemical cues presented by the native tendon extracellular matrix (ECM) and the embedded tissue-specific biofactors. In this study, we have prepared and characterized the biological activities of a soluble extract of decellularized tendon ECM (tECM) on adult adipose-derived stem cells (ASCs), on the basis of histological, biochemical, and gene expression analyses...
January 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28068879/electrospun-template-architecture-and-composition-regulate-neutrophil-netosis-in-vitro-and-in-vivo
#8
Allison E Fetz, Indira Neeli, Isaac A Rodriguez, Marko Z Radic, Gary Bowlin
Mounting evidence indicates that neutrophils, first responders to an implanted biomaterial, prime the microenvironment for recruited immune cells by secreting factors and releasing neutrophil extracellular traps (NETs) through NETosis. In this study, we investigated the role of electrospun template architecture and composition in regulating NETosis. Electrospun polydioxanone (PDO), collagen type I (COL), and blended PDO-COL templates (PC) were fabricated with small (0.25-0.35µm) and large (1.0-2.00µm) diameter fibers...
January 9, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27899064/growth-differentiation-factor-5-mediated-enhancement-of-chondrocyte-phenotype-is-inhibited-by-heparin-implications-for-the-use-of-heparin-in-the-clinic-and-in-tissue-engineering-applications
#9
Bethanie I Ayerst, Raymond A A Smith, Victor Nurcombe, Anthony J Day, Catherine L R Merry, Simon M Cool
The highly sulfated glycosaminoglycan (GAG) heparin is widely used in the clinic as an anticoagulant, and researchers are now using it to enhance stem cell expansion/differentiation protocols, as well as to improve the delivery of growth factors for tissue engineering (TE) strategies. Growth differentiation factor 5 (GDF5) belongs to the bone morphogenetic protein family of proteins and is vital for skeletal formation; however, its interaction with heparin and heparan sulfate (HS) has not been studied. We identify GDF5 as a novel heparin/HS binding protein and show that HS proteoglycans are vital in localizing GDF5 to the cell surface...
January 6, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28051358/controlled-retention-of-bmp-2-derived-peptide-on-nanofibers-based-on-mussel-inspired-adhesion-for-bone-formation
#10
Jinkyu Lee, Sajeesh Kumar Mp, Taufiq Ahmad, Min Suk Lee, Heeseok Yang, Do-Gyoon Kim, Kyobum Kim, Bosun Kwon, Heungsoo Shin
Although bone morphogenetic protein-2 (BMP-2) has been frequently used to stimulate bone formation, it has several side effects to be addressed, including the difficulty in optimization of clinically relevant doses and unwanted induction of cancerous signaling processes. In this study, an osteogenic peptide (OP) derived from BMP-2 was investigated as a substitute for BMP-2. In vitro studies showed that OP was able to enhance in the osteogenic differentiation and mineralization of human mesenchymal stem cells (hMSCs)...
January 4, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27923325/a-gingiva-derived-mesenchymal-stem-cell-laden-porcine-small-intestinal-submucosa-extracellular-matrix-construct-promotes-myomucosal-regeneration-of-the-tongue
#11
Qilin Xu, Rabie M Shanti, Qunzhou Zhang, Steven B Cannady, Bert W O'Malley, Anh D Le
In the oral cavity, the tongue is the anatomic subsite most commonly involved by invasive squamous cell carcinoma. Current treatment protocols often require significant tissue resection to achieve adequate negative margins and optimal local tumor control. Reconstruction of the tongue while preserving and/or restoring its critical vocal, chewing, and swallowing functions remains one of the major challenges in head and neck oncologic surgery. We investigated the in vitro feasibility of fabricating a novel combinatorial construct using porcine small intestinal submucosa extracellular matrix (SIS-ECM) and human gingiva-derived mesenchymal stem cells (GMSCs) as a GMSC/SIS-ECM tissue graft for the tongue reconstruction...
January 4, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28027709/conditioned-medium-from-periodontal-ligament-stem-cells-enhances-periodontal-regeneration
#12
Mizuki Nagata, Kengo Iwasaki, Keiko Akazawa, Motohiro Komaki, Naoki Yokoyama, Yuichi Izumi, Ikuo Morita
Periodontal disease is one of the most common infectious diseases in adults and is characterized by the destruction of tooth-supporting tissues. Mesenchymal stem cells (MSCs) comprise the mesoderm-originating stem cell population, which has been studied and used for cell therapy. However, because of the lower rate of cell survival after MSC transplantation in various disease models, paracrine factions of MSCs have been receiving increased attentions as a regenerative mechanism. The aim of this study was to investigate the regenerative potential of transplanted of conditioned medium (CM) obtained from cultured periodontal ligament stem cells (PDLSCs), the adult stem cell population in tooth-supporting tissues, using a rat periodontal defect model...
December 27, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28027692/comparison-of-3d-printed-poly-%C3%AE%C2%B5-caprolactone-scaffolds-functionalized-with-tricalcium-phosphate-hydroxyapatite-bio-oss-or-decellularized-bone-matrix
#13
Ethan Nyberg, Alexandra Rindone, Amir Dorafshar, Warren L Grayson
3D-printing facilitates rapid, custom manufacturing of bone scaffolds with a wide range of material choices. Recent studies have demonstrated the potential for 3D-printing bioactive (i.e. osteo-inductive) scaffolds for use in bone regeneration applications. In this study, we 3D-printed porous poly-ϵ-caprolactone (PCL) scaffolds using a fused deposition modeling (FDM) process and functionalized them with mineral additives that have been widely used commercially and clinically: tricalcium phosphate (TCP), hydroxyapatite (HA), Bio-Oss (BO) or decellularized bone matrix (DCB)...
December 27, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27835935/a-new-vertebral-body-replacement-strategy-using-expandable-polymeric-cages
#14
Xifeng Liu, Alex Paulsen, Hugo Giambini, Ji Guo, A Lee Miller, Po-Chun Lin, Michael J Yaszemski, Lichun Lu
We have developed a novel polymeric expandable cage that can be delivered via a posterior-only surgical approach for the treatment of noncontained vertebral defects. This approach is less invasive than an anterior-only or combined approach and much more cost-effective than currently used expandable metal cages. The polymeric expandable cage is composed of oligo poly(ethylene glycol) fumarate (OPF), a hydrogel that has been previously shown to have excellent nerve and bone tissue biocompatibility. OPF hydrogel cages can expand to twice their original diameter and length within a surgical time frame following hydration...
December 26, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27998245/generation-endothelialization-and-microsurgical-suture-anastomosis-of-strong-1-mm-diameter-collagen-tubes
#15
Xuanyue Li, Jing Xu, Calin Nicolescu, Jordann Marinelli, Joe Tien
Tissue-engineered vascular grafts that are based on reconstituted extracellular matrices have been plagued by weak mechanical strength that prevents handling or anastomosis to native vessels. In this study, we devise a method for making dense, suturable collagen tubular constructs of diameter ≤ 1 mm for potential microsurgical applications, by dehydrating tubes of native rat tail type I collagen and crosslinking them with 20 mM genipin. Crosslinked dense collagen tubes with 1 mm inner diameter yielded ultimate tensile strength of 342 ± 15 gram-force and burst pressure of 1313 ± 156 mm Hg, comparable to the strength of a rat femoral artery, and supported endothelial cell adhesion and growth...
December 21, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27998239/bone-tissue-engineering-under-xenogeneic-free-conditions-in-a-large-animal-model-as-a-basis-for-early-clinical-applicability
#16
Annika Weigand, Justus Patrick Beier, Rafael Schmid, Tobias Knorr, David Kilian, Rebekka Götzl, Thomas Gerber, Raymund E Horch, Anja Miriam Boos
For decades, researchers have been developing a range of promising strategies in bone tissue engineering with the aim of producing a significant clinical benefit over existing therapies. However, a major problem concerns the traditional use of xenogeneic substances for the expansion of cells, which complicates direct clinical transfer. The study's aim was to establish a totally autologous sheep model as a basis for further preclinical studies and future clinical application. Ovine MSC were cultivated in different concentrations (0 %, 2 %, 5 %, 10 %, 25 %) of either autologous serum (AS) or fetal calf serum (FCS)...
December 21, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27998214/fabrication-of-a-highly-aligned-neural-scaffold-via-a-table-top-stereolithography-3d-printing-and-electrospinning
#17
Se-Jun Lee, Margaret Nowicki, Brent Harris, Lijie Grace Zhang
3D bioprinting is a rapidly emerging technique in the field of tissue engineering to fabricate extremely intricate and complex biomimetic scaffolds in the range of micrometers. Such customized 3D printed constructs can be used for the regeneration of complex tissues such as cartilage, vessels and nerves. However, the 3D printing techniques often offer limited control over the resolution and compromised mechanical properties due to short selection of printable inks. To address these limitation, we combined stereolithography and electrospinning techniques to fabricate a novel 3D biomimetic neural scaffold with a tunable porous structure and embedded aligned fibers...
December 21, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27869543/direct-bonding-of-chitosan-biomaterials-to-tissues-using-transglutaminase-for-surgical-repair-or-device-implantation
#18
Javier G Fernandez, Suneil Seetharam, Christopher Ding, Juani Feliz, Ed Doherty, Donald E Ingber
Natural biomaterials, such as chitosan and collagen, are useful for biomedical applications because they are biocompatible, mechanically robust, and biodegradable, but it is difficult to rapidly and tightly bond them to living tissues. In this study, we demonstrate that the microbial transglutaminase (mTG), can be used to rapidly (<5 min) bond chitosan and collagen biomaterials to the surfaces of hepatic, cardiac, and dermal tissues, as well as to functionalized polydimethylsiloxane (PDMS) materials that are used in medical products...
December 19, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27824284/formation-of-hyaline-cartilage-tissue-by-passaged-human-osteoarthritic-chondrocytes
#19
Vanessa J Bianchi, Joanna F Weber, Stephen D Waldman, David Backstein, Rita A Kandel
OBJECTIVE: When serially passaged in standard monolayer culture to expand cell number, articular chondrocytes lose their phenotype. This results in the formation of fibrocartilage when they are used clinically, thus limiting their use for cartilage repair therapies. Identifying a way to redifferentiate these cells in vitro is critical if they are to be used successfully. Transforming growth factor beta (TGFβ) family members are known to be crucial for regulating differentiation of fetal limb mesenchymal cells and mesenchymal stromal cells to chondrocytes...
December 19, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27967655/encapsulation-of-adenovirus-bmp2-transduced-cells-with-pegda-hydrogels-allows-bone-formation-in-the-presence-of-immune-response
#20
Pedro Alvarez Urena, Eleanor Davis, Corinne Sonnet, Gabrielle Henslee, Zbigniew Gugala, Edward V Strecker, Laura J Linscheid, Maude L Cuchiara, Jennifer L West, Alan R Davis, Elizabeth Olmsted-Davis
Gene therapy approaches have been difficult to implement due to pre-existing immunity against the virus used for delivery. To circumvent this problem, a cell-based approach was developed that avoided the use of free virus within the animal. However, even cells transduced in vitro with E1-E3-deleted adenovirus encoding bone morphogenetic protein 2 (AdBMP2) resulted in production of virus neutralizing antibodies in mice. Further, when mice received an intramuscular injection of nonencoding adenovirus (AdEmpty)-transduced cells, AdBMP2-transduced cells were unable to launch bone formation when an intramuscular injection of these BMP2-producing cells was delivered one week later...
December 14, 2016: Tissue Engineering. Part A
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