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Tissue Engineering. Part A

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https://www.readbyqxmd.com/read/28816097/meltblown-polymer-fabrics-as-candidate-scaffolds-for-rotator-cuff-tendon-tissue-engineering
#1
Thomas Jenkins, Sean Meehan, Behnam Pourdeyhimi, Dianne Little
Various biomaterial technologies are promising for tissue engineering, including electrospinning, but commercial scale-up of throughput is difficult. The goal of the study was to evaluate meltblown fabrics as candidate scaffolds for rotator cuff tendon tissue engineering. Meltblown poly(lactic acid) fabrics were produced with several polymer crystallinities and airflow velocities (500(low), 900(medium) or 1400(high) m<sup>3</sup>air/hour/m fabric). Fiber diameter, alignment, and baseline bi-directional tensile mechanical properties were evaluated...
August 17, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28602124/composite-scaffolds-of-mineralized-natural-extracellular-matrix-on-true-bone-ceramic-induce-bone-regeneration-through-smad1-5-8-and-erk1-2-pathways
#2
Tingfang Sun, Sheng Yao, Man Liu, Yushi Yang, Yanhui Ji, Wei Cui, Yanzhen Qu, Xiaodong Guo
A stem cell-derived mineralized extracellular matrix (ECM) may be a good strategy to endow scaffolds with a bone microenvironment, thus inducing bone regeneration. However, it also faces some challenges, such as limited number of cells, additional wound for autologous cell isolation, and time consumption of cell expansion. In this study, we designed a novel tissue-derived ECM scaffold fabricated by depositing porcine small intestinal submucosa (SIS) ECM on true bone ceramic (TBC), which was followed by mineralization treatment (mSIS/TBC)...
August 15, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28806146/three-dimensional-bioprinting-of-polycaprolactone-reinforced-gene-activated-bioinks-for-bone-tissue-engineering
#3
Gráinne M Cunniffe, Tomas Gonzalez-Fernandez, Andrew Daly, Binulal N Sathy, Oju Jeon, Eben Alsberg, Daniel J Kelly
Regeneration of complex bone defects remains a significant clinical challenge. Multi-tool biofabrication has permitted the combination of various biomaterials to create multifaceted composites with tailorable mechanical properties and spatially controlled biological function. In this study we sought to use bioprinting to engineer nonviral gene activated constructs reinforced by polymeric micro-filaments. A gene activated bioink was developed using RGD-γ-irradiated alginate and nano-hydroxyapatite (nHA) complexed to plasmid DNA (pDNA)...
August 14, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28793839/roughness-and-hydrophilicity-as-osteogenic-biomimetic-surface-properties
#4
Barbara Boyan, Ethan Major Lotz, Zvi Schwartz
Successful dental and orthopaedic implant outcomes are determined by the degree of osseointegration. Over the last 60 years, endosseous implants have evolved to stimulate osteogenesis without the need for exogenous biologics like bone morphogenetic proteins. An understanding of the interaction between cells and how cells interact with the physical features characteristics of their environments have has led to the development ofse bioactive implants. Implant surfaces that mimic the inherent chemistry, topography, and hydrophilicity wettability of native bone have been shown to provide cells in the osteoblast lineage cells with the structural cues to promote tissue regeneration and net new bone formation...
August 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28793833/synergistic-therapeutic-effect-of-three-dimensional-stem-cell-clusters-and-angiopoietin-1-on-promoting-vascular-regeneration-in-ischemic-region
#5
Jungmi Kang, Jeong-Kee Yoon, Seung Ja Oh, Byung-Soo Kim, Sang-Heon Kim
Peripheral artery disease (PAD) is an ischemic disease characterized by reduced blood flow to the legs, feet, and hands. Human mesenchymal stem cells (MSCs) are an attractive cell source to treat PAD in regenerative medicine. However, in clinical applications, the use of adult stem cells has several limitations, such as low cell viability and low therapeutic efficiency. In the present study, we described an innovative method of culturing three-dimensional stem cell clusters (AngioclusterTM, AC), demonstrated the potential for ACs to differentiate into vascular cells, and assessed the synergistic effects of ACs and angiopoietin-1 (Ang-1) on angiogenesis in ischemic animal models...
August 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28783453/development-of-human-salivary-gland-like-tissue-in-vitro
#6
Marc Burghartz, Simon Lennartz, Matthias Schweinlin, Rudolf Hagen, Norbert Kleinsasser, Stephan Hackenberg, Gudrun Steußloff, Agmal Scherzad, Kathrin Radeloff, Christian Ginzkey, Heike Walles, Marco Metzger
The loss of salivary gland function caused by radiation therapy of the head and neck is a serious condition and it affects a patient's quality of life. The current lack of effective therapies demands new options to be explored. This study tested whether human salivary gland epithelial cells (SGECs) could be successfully cultured on a decellularized porcine gut matrix (SIS-muc) in both mono- and coculture with microvascular endothelial cells (mvECs). By performing immunofluorescence imaging, transmission as well as scanning electron microscopy (SEM), quantitative polymerase chain reaction (qPCR), and an amylase enzyme assay, it was investigated as to what extent the three-dimensional (3D)-cultured cells could maintain their molecular differentiation and the production of working α-amylase (α-AMY) compared with two-dimensional (2D) culture...
August 4, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28762866/in-vitro-bioactivity-of-one-and-two-dimensional-nanoparticle-incorporated-bone-tissue-engineering-scaffolds
#7
Jason Thomas Rashkow, Gaurav Lalwani, Balaji Sitharaman
This study investigates the effect of incorporation of one- or two-dimensional nanoparticles with distinct composition and morphology on the bioactivity of biodegradable, biocompatible polymer matrices. 0.2 wt% multiwalled carbon nanotubes (MWCNTs), multiwalled graphene nanoribbons (MWGONRs), graphene nanoplatelets (GONPs), molybdenum disulfide nanoplatelets (MSNPs), or tungsten disulfide nanotubes (WSNTs) were uniformly dispersed in poly(lactic-co-glycolic acid) (PLGA) polymer. PLGA or nanoparticle incorporated PLGA were then incubated with simulated body fluid (SBF) under physiological conditions for 1, 3, 7, or 14 days...
August 1, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28649910/extracellular-vesicles-and-their-versatile-roles-in-tissue-engineering
#8
Steven M Jay, Gordana Vunjak-Novakovic
No abstract text is available yet for this article.
August 1, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28756737/mimicking-the-biochemical-and-mechanical-extracellular-environment-of-the-endochondral-ossification-process-to-enhance-the-in-vitro-mineralization-potential-of-human-mscs
#9
Fiona E Freeman, Jessica Schiavi, Meadhbh A Brennan, Peter Owens, Pierre Layrolle, Laoise McNamara
Chondrogenesis and mechanical stimulation of the cartilage template, are essential for bone formation via the endochondral ossification process in vivo. Recent studies have demonstrated that in vitro regeneration strategies that mimic these aspects separately, either chondrogenesis or mechanical stimulation, can promote mineralization to a certain extent both in vitro and in vivo. However, to date no study has sought to incorporate both the formation of the cartilage template and the application of mechanical stimulation simultaneously to induce osteogenesis...
July 29, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28602122/transcriptome-wide-analyses-of-human-neonatal-articular-cartilage-and-human-mesenchymal-stem-cell-derived-cartilage-provide-a-new-molecular-target-for-evaluating-engineered-cartilage
#10
Rodrigo A Somoza, Diego Correa, Ivan Labat, Hal Sternberg, Megan E Forrest, Ahmad M Khalil, Michael D West, Paul Tesar, Arnold I Caplan
Cellular differentiation comprises a progressive, multistep program that drives cells to fabricate a tissue with specific and site distinctive structural and functional properties. Cartilage constitutes one of the potential differentiation lineages that mesenchymal stem cells (MSCs) can follow under the guidance of specific bioactive agents. Single agents such as transforming growth factor beta (TGF-β) and bone morphogenetic protein 2 in unchanging culture conditions have been historically used to induce in vitro chondrogenic differentiation of MSCs...
July 28, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28610547/human-plasma-rich-in-growth-factors-improves-survival-and-neurite-outgrowth-of-spiral-ganglion-neurons-in-vitro
#11
Michael Stolle, Jennifer Schulze, Ariane Roemer, Thomas Lenarz, Martin Durisin, Athanasia Warnecke
BACKGROUND: Platelet-rich and platelet-poor plasma (PRP and PPP) are autologous preparations from peripheral blood and contain several growth factors and cytokines involved in tissue repair. Although their neuroprotective and neuroregenerative properties have been already described, little is known about their effects in the inner ear. We, therefore, examined the effects of PRP and PPP on spiral ganglion neurons (SGN) in vitro. RESULTS: For all experiments, spiral ganglia were isolated from neonatal rats and were cultured in serum-free medium...
July 27, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28610471/mechanically-loading-cell-hydrogel-constructs-with-low-intensity-pulsed-ultrasound-for-bone-repair
#12
James A Veronick, Fayekah Assanah, Nicole Piscopo, Yasemin Kutes, Varun Vyas, Lakshmi S Nair, Bryan D Huey, Yusuf Khan
Low-intensity pulsed ultrasound (LIPUS) has been shown to be effective for orthopedic fracture repair and nonunion defects, but the specific mechanism behind its efficacy is still unknown. Previously, we have shown a measurable acoustic radiation force at LIPUS intensities traditionally used for clinical treatment and have applied this force to osteoblastic cells encapsulated in type I collagen hydrogels. Our goal in this study is to provide insight and inform the appropriate design of a cell therapy approach to bone repair in which osteoblasts are embedded in collagen hydrogels, implanted into a bony defect, and then transdermally stimulated using LIPUS-derived acoustic radiation force to enhance bone formation at the earliest time points after bone defect repair...
July 27, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28741418/focal-adhesion-kinase-activation-is-necessary-for-stretch-induced-alignment-and-enhanced-differentiation-of-myogenic-precursor-cells
#13
Jens Isak Andersen, Cristian Pablo Pennisi, Trine Fink, Vladimir Zachar
Myogenic precursors sense and dynamically respond to mechanical stimulation through complex integrin-mediated mechanotransduction, in which focal adhesion kinase (FAK) is a fundamental intracellular signaling mediator. When skeletal myoblasts are exposed to uniaxial cyclic tensile strain (UCTS) they display uniform alignment and an enhanced rate of differentiation. In this work, we explored the role of FAK activation using C2C12 myoblasts that were grown on flexible culture plates and exposed to UCTS during the early differentiation phase...
July 25, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28741412/directly-induced-neural-differentiation-of-human-adipose-derived-stem-cells-using-3d-culture-system-of-conductive-microwell-with-electrical-stimulation
#14
Dong Nyung Heo, Nana Acquah, Junghoon Kim, Se-Jun Lee, Nathan J Castro, Lijie Grace Zhang
Adipose-derived stem cells (ADSCs) have the capacity to differentiate into neural precursor cells which can be used for nerve regeneration. However, their inherently low neurogenic differentiation efficiency limits further clinical applications. This study was designed to promote neurogenic differentiation efficacy of ADSCs via integrating conductive hydrogel-based microwells with electrical stimulation (ES). We hypothesize that ADSCs will differentiate more efficiently into neural precursor cells when electrically stimulated in conductive hydrogel microwells...
July 25, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28463605/comparison-of-poly-l-lactide-co-%C3%A9-caprolactone-and-poly-trimethylene-carbonate-membranes-for-urethral-regeneration-an-in-vitro-and-in-vivo-study
#15
Reetta Sartoneva, Panu H Nordback, Suvi Haimi, Dirk W Grijpma, Kalle Lehto, Niall Rooney, Riitta Seppänen-Kaijansinkko, Susanna Miettinen, Tuija Lahdes-Vasama
Urethral defects are normally reconstructed using a patient's own genital tissue; however, in severe cases, additional grafts are needed. We studied the suitability of poly(l-lactide-co-ɛ-caprolactone) (PLCL) and poly(trimethylene carbonate) (PTMC) membranes for urethral reconstruction in vivo. Further, the compatibility of the materials was evaluated in vitro with human urothelial cells (hUCs). The attachment and viability of hUCs and the expression of different urothelial cell markers (cytokeratin 7, 8, 19, and uroplakin Ia, Ib, and III) were studied after in vitro cell culture on PLCL and PTMC...
July 21, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28726588/reconstituting-mouse-lungs-with-conditionally-reprogrammed-human-bronchial-epithelial-cells
#16
Ryan LaRanger, Jennifer R Peters-Hall, Melissa Coquelin, Busola R Alabi, Chris Chen, Woodring E Wright, Jerry Shay
We developed methods for conditionally reprogramming (CR) primary human bronchial epithelial cells (HBECs) to extend their functional life span and permit their differentiation into both upper and lower airway lung epithelium. We also developed a bioreactor to support vascular perfusion and rhythmic breathing of decellularized mouse lungs reconstituted with CR HBECs isolated from patients with and without cystic fibrosis (CF). While conditionally reprogrammed cells only differentiate into an upper airway epithelium after 35 days at the air liquid interface, in reconstituted lungs these cells differentiate into upper airway bronchial epithelium and lower airway alveolar structures after 12 days...
July 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28726587/repair-of-tympanic-membrane-perforations-with-customized-bioprinted-ear-grafts-using-chinchilla-models
#17
Che-Ying Kuo, Emmanuel Wilson, Andrew Fuson, Nidhi Gandhi, Reza Monfaredi, Audrey Jenkins, Maria Romero, Marco Santoro, John P Fisher, Kevin Cleary, Brian Reilly
The goal of this work is to develop an innovative method that combines bioprinting and endoscopic imaging to repair tympanic membrane perforations (TMPs). TMPs are a serious health issue because they can lead to both conductive hearing loss and repeated otitis media. TMPs occur in 3 to 5% of cases after ear tube placement as well as in cases of acute otitis media (the second most common infection in pediatrics), chronic otitis media with or without cholesteatoma, or as a result of barotrauma to the ear. About 55,000 tympanoplasties, the surgery performed to reconstruct TMPs, are performed every year and the commonly used cartilage grafting technique has a success rate between 43% to 100%...
July 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28726579/the-immunomodulatory-role-of-bmp-2-on-macrophages-to-accelerate-osteogenesis
#18
Fei Wei, Yinghong Zhou, Jing Wang, Changsheng Liu, Yin Xiao
Bone morphogenetic protein-2 (BMP-2) is one of the most frequently used osteogenic factors for osteogenesis. Macrophages, acting as both immune cells and osteoclast precursors, have an indispensable functional input during multiple stages of bone healing. This study aims to investigate the immunoregulatory role of BMP-2 on macrophages and the subsequent effects on osteogenesis. In the subcutaneous implantation study, gelatin sponge incorporated with 20 μg/mL BMP-2 rendered significantly enhanced macrophage infiltration compared to the gelatin sponge control...
July 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28726551/extracellular-matrix-and-integrin-expression-profiles-in-fuchs-endothelial-corneal-dystrophy-cells-and-tissue-model
#19
Benjamin Goyer, Mathieu Thériault, Sébastien P Gendron, Isabelle Brunette, Patrick J Rochette, Stéphanie Proulx
Primary corneal endothelial cell cultures and 3D engineered tissue models were used to study the aberrant deposition of extracellular matrix (ECM) in a vision impairing pathology known as Fuchs endothelial corneal dystrophy (FECD). Corneal endothelial cells (CEC) were isolated from excised Descemet membranes of patients with end-stage FECD. CEC isolated from healthy corneas served as controls. Microarray gene profiling was performed on post-confluent cultures of healthy and FECD cells. Protein expression analyses were conducted on tissue models that were engineered by seeding an endothelium on previously devitalized human stromal carriers...
July 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28726548/wnt-yap-interactions-during-neural-tissue-patterning-of-human-induced-pluripotent-stem-cells
#20
Julie Bejoy, Liqing Song, Yi Zhou, Yan Li
Human induced pluripotent stem cells (hiPSCs) have special ability to self-assemble into neural spheroids or mini-brain like structures. During the self-assembly process, Wnt signaling plays an important role in regional patterning and establishing positional identity of hiPSC-derived neural progenitors. Recently, the role of Wnt signaling in regulating Yes-associated protein (YAP) expression (nuclear or cytoplasmic), the pivotal regulator during organ growth and tissue generation, has attracted increasing interests...
July 20, 2017: Tissue Engineering. Part A
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