journal
MENU ▼
Read by QxMD icon Read
search

Tissue Engineering. Part A

journal
https://www.readbyqxmd.com/read/28637399/biomineralized-recombinant-collagen-based-scaffold-mimicking-native-bone-enhances-mesenchymal-stem-cell-interaction-and-differentiation
#1
Gloria Belén Ramirez-Rodríguez, Monica Montesi, Silvia Panseri, Simone Sprio, Anna Tampieri, Monica Sandri
The need of synthetic bone grafts that recreate from macro to nanoscale level the biochemical and biophysical cues of bone extracellular matrix (ECM) has been a major driving force for the development of new generation of biomaterials. In this study, synthetic bone substitutes have been synthesized via biomimetic mineralization of a recombinant collagen type I derived peptide (RCP), enriched in tri-amino acid sequence arginine-glycine-aspartate (RGD). 3D isotropic porous scaffolds of three different compositions are developed by freeze-drying: non-mineralized (RCP, as a control), mineralized (Ap/RCP) and mineralized scaffolds in presence of magnesium (MgAp/RCP) that closely imitate bone composition...
June 22, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28622088/engineering-osteoinductive-biomaterials-by-bioinspired-synthesis-of-apatite-coatings-on-collagen-hydrogels-with-varied-pore-microarchitectures
#2
Jacqueline Lorraine Harding, Matthew Osmond, Melissa Diane Krebs
Biomaterial controlled osteoinduction is influenced by the porous microenvironment and the composition of incorporated calcium orthophosphate (CaPi) polymorphs, however for the design of materials that rival the efficacies of natural grafts a systematic approach to assessing the physicochemical properties that affects cellular differentiation is needed. In this research, we introduce a bioinspired synthetic approach to the mineralization of preformed porous collagen hydrogel scaffolds with tunable apatite coatings...
June 16, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28471299/interleukin-10-deficiency-impairs-reparative-properties-of-bone-marrow-derived-endothelial-progenitor-cell-exosomes
#3
Yujia Yue, Venkata Naga Srikanth Garikipati, Suresh Kumar Verma, David A Goukassian, Raj Kishore
Endothelial progenitor cell (EPC)-based therapy has immense potential to promote cardiac neovascularization and attenuate ischemic injury. Functional benefits of EPCs and other adult stem cell therapies largely involve paracrine mechanisms and exosomes secreted by stem cells are emerging as pivotal paracrine entity of stem/progenitor cells. However, modest outcomes after EPC-/stem cell-based clinical trials suggest that stem cell/exosome function might be modulated by stimuli they encounter in ischemic tissues, including systemic inflammation...
June 15, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28610547/human-plasma-rich-in-growth-factors-improves-survival-and-neurite-outgrowth-of-spiral-ganglion-neurons-in-vitro
#4
Michael Stolle, Jennifer Schulze, Ariane Römer, Thomas Lenarz, Martin Durisin, Athanasia Warnecke
BACKGROUND: Platelet-rich and platelet poor plasma (PRP and PPP) are autologous preparations from peripheral blood and contain several growth factors and cytokines involved in tissue repair. Although their neuroprotective and -regenerative properties have been already described, little is known about their effects in the inner ear. We therefore examined the effects of PRP and PPP on spiral ganglion neurons (SGN) in vitro. RESULTS: For all experiments, spiral ganglia were isolated from neonatal rats and were cultured in serum-free medium...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28610480/induced-re-differentiation-of-human-chondrocytes-from-articular-cartilage-lesion-in-alginate-bead-culture-after-monolayer-de-differentiation-an-alternative-cell-source-for-cell-based-therapies
#5
Matthias Aurich, Gunther O Hofmann, Norman Best, Bernd Rolauffs
Human chondrocytes isolated from articular cartilage lesions as an alternative cell source to the standard non-weight-bearing notch biopsy site may hold clinical potential for cell-based therapies. The aim was to characterize human AC lesion site chondrocytes, compare them to notch chondrocytes, and to evaluate their re-differentiation potential after monolayer expansion and subsequent 3D alginate bead culture. Lesion chondrocytes from knee joints of 20 patients with ICRS grade 3 and 4 cartilage defects were analyzed ex vivo or cultured in primary alginate bead culture, monolayer expansion, or were re-differentiated in alginate culture following monolayer expansion...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28610471/mechanically-loading-cell-hydrogel-constructs-with-low-intensity-pulsed-ultrasound-for-bone-repair
#6
James A Veronick, Fayekah Assanah, Nicole J Piscopo, Yasemin Kutes, Varun Vyas, Bryan D Huey, Yusuf Khan
Low-intensity pulsed ultrasound (LIPUS) has been shown to be effective for orthopaedic fracture repair and non-union defects but the specific mechanism behind its efficacy is still unknown. Previously we have shown a measurable acoustic radiation force at LIPUS intensities traditionally used for clinical treatment and have applied this force to osteoblastic cells encapsulated in type I collagen hydrogels. Our goal here is to provide insight and inform the appropriate design of a cell therapy approach to bone repair in which osteoblasts are embedded in collagen hydrogels, implanted into a bony defect, and then transdermally stimulated using LIPUS-derived acoustic radiation force to enhance bone formation at the earliest time points after bone defect repair...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28498040/pivotal-role-of-non-cardiomyocytes-in-electromechanical-and-therapeutic-potential-of-induced-pluripotent-stem-cell-derived-engineered-cardiac-tissue
#7
Hiroko Iseoka, Shigeru Miyagawa, Satsuki Fukushima, Atsuhiro Saito, Shigeo Masuda, Shin Yajima, Emiko Ito, Nagako Sougawa, Maki Takeda, Akima Harada, Jong-Kook Lee, Yoshiki Sawa
Although engineered cardiac tissues (ECTs) derived from induced pluripotent stem cells (iPSCs) are promising for myocardial regenerative therapy, the appropriate ratio of cardiomyocytes to non-cardiomyocytes is not fully understood. Here, we determined whether ECT-cell content is a key determinant of its structure/function, thereby affecting ECT therapeutic potential for advanced heart failure. Scaffold-free ECTs containing different ratios (25%, 50%, 70%, or 90%) of iPSC-derived cardiomyocytes were generated by magnetic-activated cell sorting by using cardiac-specific markers...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28486019/role-of-bone-marrow-mononuclear-cell-seeding-for-nanofiber-vascular-grafts
#8
Takuma Fukunishi, Cameron A Best, Chin Siang Ong, Tyler Groehl, James Reinhardt, Tai Yi, Hideki Miyachi, Huaitao Zhang, Toshiharu Shinoka, Christopher K Breuer, Jed Johnson, Narutoshi Hibino
OBJECTIVE: Electrospinning is a promising technology that provides biodegradable nanofiber scaffolds for cardiovascular tissue engineering. However, success with these materials has been limited, and the optimal combination of scaffold parameters for a tissue-engineered vascular graft (TEVG) remains elusive. The purpose of the present study is to evaluate the effect of bone marrow mononuclear cell (BM-MNC) seeding in electrospun scaffolds to support the rational design of optimized TEVGs...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28471327/renal-regenerative-potential-of-different-extracellular-vesicle-populations-derived-from-bone-marrow-mesenchymal-stromal-cells
#9
Stefania Bruno, Marta Tapparo, Federica Collino, Giulia Chiabotto, Maria Chiara Deregibus, Rafael Soares Lindoso, Francesco Neri, Sharad Kholia, Sara Giunti, Sicheng Wen, Peter Quesenberry, Giovanni Camussi
Extracellular vesicles (EVs) derived from human bone marrow mesenchymal stromal cells (MSCs) promote the regeneration of kidneys in different animal models of acute kidney injury (AKI) in a manner comparable with the cells of origin. However, due to the heterogeneity observed in the EVs isolated from MSCs, it is unclear which population is responsible for the proregenerative effects. We therefore evaluated the effect of various EV populations separated by differential ultracentrifugation (10K population enriched with microvesicles and 100K population enriched with exosomes) on AKI recovery...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28463545/meniscal-tissue-engineering-using-aligned-collagen-fibrous-scaffolds-comparison-of-different-human-cell-sources
#10
Jihye Baek, Sujata Sovani, Wonchul Choi, Sungho Jin, Shawn P Grogan, Darryl D D'Lima
Hydrogel and electrospun scaffold materials support cell attachment and neotissue development and can be tuned to structurally and mechanically resemble native extracellular matrix by altering either electrospun fiber or hydrogel properties. In this study, we examined meniscus tissue generation from different human cell sources including meniscus cells derived from vascular and avascular regions, human bone marrow-derived mesenchymal stem cells, synovial cells, and cells from the infrapatellar fat pad (IPFP)...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28602124/composite-scaffolds-of-mineralized-natural-extracellular-matrix-on-true-bone-ceramic-induce-bone-regeneration-via-smad1-5-8-and-erk1-2-pathways
#11
Tingfang Sun, Sheng Yao, Man Liu, Yushi Yang, Yanhui Ji, Wei Cui, Yanzhen Qu, Xiao-Dong Guo
A stem cell-derived mineralized extracellular matrix (ECM) may be a good strategy to endow scaffolds with a bone microenvironment, thus inducing bone regeneration. However, it also faces some challenges, such as limited cell number, additional wound for autologous cell isolation, and time consumption of cell expansion. In this study, we designed a novel tissue-derived ECM scaffold fabricated by depositing porcine small intestinal submucosa (SIS) ECM on true bone ceramic (TBC), which was followed by mineralization treatment (mSIS/TBC)...
June 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28602122/transcriptome-wide-analyses-of-human-neonatal-articular-cartilage-and-human-mesenchymal-stem-cells-hmscs-derived-cartilage-provide-a-new-molecular-target-for-evaluating-engineered-cartilage
#12
Rodrigo Alfonso Somoza, Diego Correa, Ivan Labat, Hal Sternberg, Megan E Forrest, Ahmad M Khalil, Michael D West, Paul J Tesar, Arnold I Caplan
Cellular differentiation comprises a progressive, multistep program that drives cells to fabricate a tissue with specific and site distinctive structural and functional properties. Cartilage constitutes one of the potential differentiation lineages that Mesenchymal Stem Cells (MSCs) can follow under the guidance of specific bioactive agents. Single agents such as TGF-β and BMP2 in unchanging culture conditions have been historically used to induce in vitro chondrogenic differentiation of MSCs. Despite the expression of traditional chondrogenic biomarkers such as type II collagen and aggrecan, the resulting tissue represents a transient cartilage rather than an in vivo articular cartilage (AC), differing significantly in structure, chemical composition, cellular phenotypes, and mechanical properties...
June 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28594289/hypoxic-3d-cellular-network-construction-replicates-ex-vivo-the-phenotype-of-primary-human-osteocytes
#13
Saba Choudhary, Qiaoling Sun, Ciaran Mannion, Yair Kissin, Jenny Zilberberg, Woo Young Lee
Osteocytes are deeply embedded in the mineralized matrix of bone and are non-proliferative, making them a challenge to isolate and maintain using traditional in vitro culture methods without sacrificing their inimitable phenotype. We studied the synergistic effects of two microenvironmental factors that are vital in retaining, ex vivo, the phenotype of primary human osteocytes: hypoxia and 3D cellular network. In order to recapitulate the lacunocanalicular structure of bone tissue, we assembled and cultured primary human osteocytic cells with biphasic calcium phosphate microbeads in a microfluidic perfusion culture device...
June 8, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28594288/in-vitro-maturation-of-human-ips-derived-neuroepithelial-cells-influences-transplant-survival-in-the-stroke-injured-rat-brain
#14
Samantha L Payne, Priya N Anandakumaran, Balazs V Varga, Cindi M Morshead, Andras Nagy, Molly S Shoichet
Stem cell transplantation is a promising strategy for brain tissue regeneration; yet, despite some success, cell survival following transplantation remains low. Here we demonstrate that cell viability is enhanced by control over maturation of neuronal precursor cells, which are delivered in an injectable blend of hyaluronan (HA) and methylcellulose (MC) (HAMC). We selected three subpopulations of human neuronal precursor cells derived from a cortically-specified neuroepithelial stem cell (cNESC) population based on differences in expression of multipotent and neuron-specific proteins: early, mid-, and late-differentiated neurons...
June 8, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28586292/a-systematic-evaluation-of-factors-affecting-extracellular-vesicle-uptake-by-breast-cancer-cells
#15
Devin Stranford, Michelle E Hung, Emma Gargus, Ramille N Shah, Joshua Nathaniel Leonard
Extracellular vesicles (EVs) are nanometer-scale particles that are secreted by cells and mediate intercellular communication by transferring biomolecules between cells. Harnessing this mechanism for therapeutic biomolecule delivery represents a promising frontier for regenerative medicine and other clinical applications. One challenge to realizing this goal is that to date, our understanding of which factors affect EV uptake by recipient cells remains incomplete. In this study, we systematically investigated such delivery questions in the context of breast cancer cells, which are one of the most well-studied cell types with respect to EV delivery and therefore comprise a facile model system for this investigation...
June 6, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28580875/matrix-bound-nanovesicles-recapitulate-extracellular-matrix-effects-on-macrophage-phenotype
#16
Luai Huleihel, Joseph Bartolacci, Jenna Lynn Dziki, Tatiana Vorobyov, Brooke Arnold, Michelle Scarritt, Catalina Pineda Molina, Samuel LoPresti, Bryan Brown, Stephen F Badylak
The early macrophage response to biomaterials has been shown to be a critical and predictive determinant of downstream outcomes. When properly prepared, bioscaffolds derived from mammalian extracellular matrix (ECM) have been shown to promote a transition in macrophage behavior from a pro-inflammatory to a regulatory/anti-inflammatory phenotype, which in turn has been associated with constructive and functional tissue repair. The mechanism by which ECM bioscaffolds promote this phenotypic transition, however, is poorly understood...
June 4, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28562226/inhibition-of-pathological-phenotype-of-hypertrophic-scar-fibroblasts-via-co-culture-with-adipose-derived-stem-cells
#17
Jingcheng Deng, Yuan Shi, Zhen Gao, Wen Jie Zhang, Xiaoli Wu, Weigang Cao, Wei Liu
Hypertrophic scar (HS) is a dermal fibroproliferative disease characterized by fibroblast over-proliferation, overproduction and deposition of the extracellular matrix (ECM). Growing evidence demonstrated that adipose derived stem cells (ASCs) secrete a plethora of trophic and anti-fibrotic factors, which suppress inflammation and ameliorate fibrosis of different tissues. However, few studies investigate their effect on repressing HS activity. This study evaluated the suppressing effect of ASCs on HS fibroblast bioactivity and the possible mechanism via a co-culture model...
May 31, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28562185/hypoxia-biomimicry-to-enhance-monetite-bone-defect-repair
#18
Justin Drager, Jose Luis Ramirez-GarciaLuna, Abhishek Kumar, Uwe Gbureck, Edward J Harvey, Jake Barralet
Tissue hypoxia is a critical driving force for angiogenic and osteogenic responses in bone regeneration and is, at least partly, under the control of the Hypoxia Inducible Factor-1α (HIF-1α) pathway. Recently, the widely used iron chelator Deferoxamine (DFO) has been found to elevate HIF-1α levels independent of oxygen concentrations, thereby, creating an otherwise normal environment that mimics the hypoxic state. This has the potential to augment the biological properties of inorganic scaffolds without the need of recombinant growth factors...
May 31, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28562183/extracellular-matrices-for-bone-regeneration-a-literature-review
#19
Alaa Mansour, Mohamed Amine Mezour, Zahi Badran, Faleh Tamimi
Bone regeneration is a major challenge in cranio-facial and orthopedic surgery. Several biomaterials have been developed to overcome the limitations of autogenic bone graft, the gold standard for bone regeneration. Among these materials, decellularized extracellular matrix (ECM) has offered promising outcomes in bone regeneration. The ECMs include bioactive molecules that modulate cell proliferation and differentiation, thus, providing an excellent microenvironment for cell recruitment. ECM can be derived from tissues such as bone, cartilage, skin, and developing tooth germs, Alternatively, it can also be derived in vitro from cells such as osteoblasts, chondrocytes, adipose stem cells (ASCs), mesenchymal cells (MSCs) and embryonic stem cells (ESCs)...
May 31, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28548630/tenogenic-differentiation-of-human-embryonic-stem-cells
#20
Tina P Dale, Shazia Mazher, William R Webb, Jing Zhou, Nicola Maffulli, G-Q Chen, Alicia J El Haj, Nicholas R Forsyth
Tendon healing is complex to manage because of the limited regeneration capacity of tendon tissue; stem cell-based tissue engineering approaches may provide alternative healing strategies. We sought to determine whether human embryonic stem cells (hESC) could be induced to differentiate into tendon-like cells by the addition of exogenous bone morphogenetic protein (BMP)12 (growth differentiation factor(GDF)7) and BMP13 (GDF6). hESC (SHEF-1) were maintained with or without BMP12/13 supplementation, or supplemented with BMP12/13 and the SMAD signalling cascade blocking agent, dorsomorphin...
May 26, 2017: Tissue Engineering. Part A
journal
journal
41884
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"