journal
MENU ▼
Read by QxMD icon Read
search

Tissue Engineering. Part A

journal
https://www.readbyqxmd.com/read/29448915/shear-conditioning-of-adipose-stem-cells-for-reduced-platelet-binding-to-engineered-vascular-grafts
#1
Anh La, Robert T Tranquillo
Conferring anti-thrombogenicity to tissue-engineered vascular grafts remains a major challenge, especially for urgent bypass grafting that excludes approaches based on expanding autologous endothelial cells that requires weeks of cell culture. Adipose-derived stem cells are available from most patients in sufficient number for coronary bypass graft seeding and may be effective as allogeneic cells. We thus compared the adhesion and platelet binding of human adipose-derived stem cells that were shear-conditioned with steady and pulsatile shear stress after seeding the cells on a biologically-engineered matrix suitable for arterial grafts...
February 16, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29448888/cross-linked-cholecyst-derived-extracellular-matrix-for-abdominal-wall-repair
#2
Jeffrey Chee Yean Chan, Krishna Burugapalli, Yi-Shiang Huang, Jack L Kelly, Abhay Pandit
Abdominal wall repair frequently utilizes either non-degradable or bio-degradable meshes, which are found to stimulate undesirable biological tissue responses or which possess suboptimal degradation rate. In this study, a biologic mesh prototype made from carbodiimide-cross-linked cholecyst-derived extracellular matrix (EDCxCEM) was compared with small intestinal submucosa (Surgisis®), cross-linked bovine pericardium (Peri-Guard®), and polypropylene (Prolene®) meshes in an in vivo rabbit model. The macroscopic appearance and stereological parameters of the meshes were evaluated...
February 16, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29431053/nanoparticle-mediated-cell-capture-enables-rapid-endothelialization-of-a-novel-bare-metal-stent
#3
Brandon J Tefft, Susheil Uthamaraj, Adriana Harbuzariu, Jonathan Harburn, Tyra A Witt, Brant Newman, Peter J Psaltis, Ota Hlinomaz, David R Holmes, Rajiv Gulati, Robert D Simari, Dan Dragomir-Daescu, Gurpreet S Sandhu
Incomplete endothelialization of intracoronary stents has been associated with stent thrombosis and recurrent symptoms, whereas prolonged use of dual antiplatelet therapy increases bleeding related adverse events. Facilitated endothelialization has the potential to improve clinical outcomes in patients who are unable to tolerate dual antiplatelet therapy. The objective of this study is to demonstrate the feasibility of magnetic cell capture to rapidly endothelialize intracoronary stents in a large animal model...
February 12, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29431032/decellularized-muscle-supports-new-muscle-fibers-and-improves-function-following-volumetric-injury
#4
Michael James McClure, David Josh Cohen, Allison N Ramey, Caroline B Bivens, Satya Mallu, Jonathan E Isaacs, Emily Imming, Yen-Chen Huang, MoonHae Sunwoo, Zvi Schwartz, Barbara Boyan
Current strategies to treat volumetric muscle loss use primarily pedicle or free muscle transfers, but these grafts fail to adequately regenerate functional tissue. Decellularized soft tissue grafts possess physical and chemical cues to promote muscle regeneration, suggesting their potential for use in large muscle defects. In this study, we developed a decellularized muscle matrix (DMM) graft using rat gastrocnemius. Anisotropy and chemical components of the extracellular matrix were retained, including laminin, fibronectin, and collagen...
February 12, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29431029/oversized-biodegradable-arterial-grafts-promote-enhanced-neointimal-tissue-formation
#5
Cameron A Best, Takuma Fukunishi, Joseph Drews, Ramak Khosravi, Kan Hor, Nathan Mahler, Tai Yi, Jay D Humphrey, Jed Johnson, Christopher K Breuer, Narutoshi Hibino
Most tissue-engineered arterial grafts are complicated by aneurysmal dilation secondary to insufficient neotissue formation after scaffold degradation. The optimal graft would form an organized multi-layered structure with a robust extracellular matrix that could withstand arterial pressure. The purpose of the current study was to determine how oversizing a biodegradable arterial scaffold affects long-term neotissue formation. Size-matched (1.0 mm, n=11) and oversized (1.6 mm, n=9) electrospun polycaprolactone/chitosan scaffolds were implanted as abdominal aortic interposition grafts in Lewis rats...
February 12, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29415631/silk-biomaterials-mediated-mirna-functionalized-orthopedic-devices
#6
Eric James, Emily Van Doren, Chunmei Li, David L Kaplan
Silk-based bioresorbable medical devices, such as screws, plates and rods, have been under investigation due to their promising properties for orthopedic repairs. Options to functionalize these devices for enhanced control of bone regeneration would also exploit the compatible processing methods used to generate the devices. MicroRNAs are important regulators of bone maintenance and formation, and miRNA-based therapeutics have the potential to aid bone repair, utilizing a transient therapeutic approach with local bioactivity...
February 7, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29397789/rock-inhibition-promotes-development-of-chondrogenic-tissue-by-improved-mass-transport
#7
Kuo-Chen Wang, Thomas T Egelhoff, Arnold I Caplan, Jean F Welter, Harihara Baskaran
Human mesenchymal stem cell (hMSC)-based chondrogenesis is a key process used to develop tissue engineered cartilage constructs from stem cells, but the resulting constructs have inferior biochemical and biomechanical properties compared to native articular cartilage. Transforming growth factor β containing medium is commonly applied to cell layers of hMSCs, which aggregate upon centrifugation to form 3-D constructs. The aggregation process leads to a high cell density condition, which can cause nutrient limitations during long-term culture, and subsequently, inferior quality of tissue engineered constructs...
February 3, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29383981/comparison-of-different-in-vivo-incubation-sites-to-produce-tissue-engineered-small-intestine
#8
Yanchun Liu, Barrett P Cromeens, Yijie Wang, Kelli Fisher, Jed Johnson, Jason Chakroff, Gail E Besner
OBJECTIVE: The objective of this study was to compare the impact of different in vivo incubation sites on the production of tissue engineered small intestine (TESI). METHODS: Green fluorescent protein (GFP) transgenic rat pups (3-5 days) were used as donors of intestinal organoids. Harvested intestine was exposed to enzymatic digestion to release intestinal stem cell-containing organoids. Organoids were purified, concentrated, and seeded onto tubular polyglycolic acid (PGA) scaffolds...
January 31, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29376483/gold-nanoparticle-collagen-gels-for-soft-tissue-augmentation
#9
Sheila A Grant, Jiaxun Zhu, Jonathan Gootee, Colten L Snider, Mitch Bellrichard, David A Grant
Collagen soft tissue fillers suffer from fast reabsorption, which minimizes their use as a tissue engineered construct. Extensive cross-linking can be utilized to extend longevity, but changes in microstructure and biomechanics can have deleterious effects. To enhance longevity while still achieving a natural microstructure, gold nanoparticles (AuNPs) were conjugated to fibrilized collagen and homogenized into an injectable form for use as a soft tissue filler. A long-term animal study in Yucatan swine was conducted to assess biocompatibility and longevity...
January 27, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29376480/fluorination-enhances-osteogenic-capacity-of-porcine-hydroxyapatite
#10
Runheng Liu, Wei Qiao, Baoxin Huang, Zetao Chen, Jinghan Fang, Zhipeng Li, Zhuofan Chen
INTRODUCTION: In a previous study, we successfully prepared fluorinated porcine hydroxyapatite (FPHA) by immersing porcine hydroxyapatite (PHA) in an aqueous solution of 0.25 M sodium fluoride (NaF) under thermal treatment, and the resulting FPHA showed better physicochemical and biological properties than PHA. The purpose of this study was to further investigate how fluorine incorporation influenced the biocompatibility and osteogenic capacity of PHA. MATERIALS AND METHODS: The concentrations of Ca, P, F, and Mg ions in PHA and FPHA extracts were detected by ICP-OES...
January 27, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29376477/immunologic-control-of-allogeneic-mesenchmal-stem-cells-improves-osteogenesis
#11
Zhigang Rong, Fei Zhang, Zhengdong Wang, Weifeng He, Shi-Wu Dong, Jianzhong Xu, Fei Dai
Use of allogeneic mesenchymal stem cells (allo-MSCs) in bone tissue engineering strategies can overcome the limitations associated with autologous MSCs, but unfortunately, the immunogenicity of allo-MSCs leads to a high rate of rejection, unless immunosuppressive agents are used. B and T lymphocyte attenuator (BTLA) is a newly discovered immunoglobulin superfamily inhibitory receptor, and Herpesvirus-entry mediator (HVEM), a member of the TNFR family, is the only ligand of BTLA. Both BTLA and HVEM are widely expressed in B and T lymphocytes and other immune cells and play significant roles in the negative regulation of an immunoreaction...
January 27, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29373945/perfusion-enhances-hypertrophic-chondrocyte-matrix-deposition-but-not-the-bone-formation
#12
Jonathan C Bernhard, Elizabeth Hulphers, Bernhard Rieder, James Crawford Ferguson, Dominik Rünzler, Thomas Nau, Heinz Redl, Gordana Vunjak-Novakovic
Perfusion bioreactors have been an effective tool in bone tissue engineering. Improved nutrient delivery and the application of shear forces have stimulated osteoblast differentiation and matrix production, allowing for generation of large, clinically-sized constructs. Differentiation of hypertrophic chondrocytes has been considered as an alternative strategy for bone tissue engineering. We studied the effects of perfusion on engineered constructs formed from human chondrocytes with respect to hypertrophic differentiation, matrix production, and subsequent bone formation...
January 26, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29368582/mineralized-biomaterials-mediated-repair-of-bone-defects-through-endogenous-cells
#13
Eva Gonzalez Diaz, Yuru Shih, Manando Nakasaki, Mengqian Liu, Shyni Varghese
Synthetic biomaterials that create a dynamic calcium (Ca2+), phosphate (PO43-) ions, and calcium phosphate (CaP)-rich microenvironment, similar to that found in native bone tissue, have been shown to promote osteogenic commitment of stem cells in vitro and in vivo. The intrinsic osteoconductivity and osteoinductivity of such biomaterials makes them promising bone grafts for the treatment of bone defects. We thus aimed to evaluate the potential of mineralized biomaterials to induce bone repair of a critical-sized cranial defect in the absence of exogenous cells and growth factors...
January 25, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29361890/modelling-neurodegenerative-microenvironment-using-cortical-organoids-derived-from-human-stem-cells
#14
Yuanwei Yan, Liqing Song, Julie Bejoy, Jing Zhao, Takahisa Kanekiyo, Guojun Bu, Yi Zhou, Yan Li
Alzheimer's disease (AD) is one of the most common neurodegenerative disorders and causes cognitive impairment and memory deficits of the patients. The mechanism of AD is not well known, due to lack of human brain models. Recently, mini-brain tissues called organoids have been derived from human induced pluripotent stem cells (hiPSCs) for modeling human brain development and neurological diseases. Thus, the objective of this research is to model and characterize neural degeneration microenvironment using 3-D forebrain cortical organoids derived from hiPSCs and study the response to the drug treatment...
January 23, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29350101/biomimetic-composite-scaffold-sis-mbg-exhibits-high-osteogenic-and-angiogenic-capacity
#15
Tingfang Sun, Man Liu, Sheng Yao, Yanhui Ji, Zekang Xiong, Kai Tang, Kaifang Chen, Hu Yang, Xiao-Dong Guo
Biomaterials with excellent osteogenic and angiogenic activities are desirable to repair massive bone defects. Decellularized matrix from porcine small intestinal submucosa (SIS) has attracted particular attention for tissue regeneration because it has strong angiogenic effects and retains plentiful bioactive components. However, it has inferior osteoinductivity and osteoconductivity. In this study, we developed porous composite of SIS combined with mesoporous bioactive glass (SIS/MBG) with the goal of improving the mechanical and biological properties...
January 19, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29343182/fibronectin-enhances-cartilage-repair-by-activating-progenitor-cells-through-integrin-%C3%A9-5%C3%AE-1-receptor
#16
Tianqi Tao, Yang Li, Chang Gui, Yong Ma, Yingbin Ge, Hanhao Dai, Kaibin Zhang, Jing Du, Yang Guo, Yiqiu Jiang, Jianchao Gui
This study aimed to determine the effect of fibronectin (FN) on cartilage regeneration through the activation of chondrogenic progenitor cells (CPCs). Cells were isolated from the knee cartilage of mice and cultured in the presence of various concentrations of FN. Proliferation, migration and chondrogenic differentiation assays were performed in vitro. In some experiments, CPCs were pre-incubated with anti-integrin ɑ5β1 antibody for 60 min prior to FN treatment to block the integrin ɑ5β1 receptor. Soluble FN was mixed with Pluronic F-127 and injected into the joint cavity in an early-stage osteoarthritis (OA) model...
January 18, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29327677/autologous-smooth-muscle-progenitor-cells-enhance-regeneration-of-tissue-engineered-bladder
#17
Liuhua Zhou, Jiadong Xia, Pengji Wang, Ruipeng Jia, Jun Hua Zheng, Xudong Yao, Yun Chen, Yutian Dai, Bin Yang
Tissue engineering techniques provide a great potential to de novo construct a histological bladder. Smooth muscle regeneration is extremely important for the functional recovery of engineered neo-bladder. However, many challenges remain for the use of bladder smooth muscle cells (SMCs) as the cell sources. Recent evidences showed that smooth muscle progenitor cells (SPCs) in the peripheral blood have the capacity of differentiating into SMCs, while their use for bladder regeneration hasn't yet been reported...
January 12, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29318958/a-cell-engineered-small-intestinal-submucosa-based-bone-mimetic-construct-for-bone-regeneration
#18
Mei Li, Chi Zhang, Yuxing Mao, Yi Zhong, Jiyuan Zhao
Extracellular matrix (ECM)-ornamented biomaterials have attracted attention due to their high potential to improve the bio-functionality of original materials. It is thought that ECM with a bone mimetic microenvironment generated by the specific induction of osteoblasts would be more beneficial for bone regeneration than regular ECM. In this study, we developed an osteogenic and mineralized ECM construct (Os/M-ECM-SIS) under the guidance of osteoblasts on a small intestinal submucosa (SIS) scaffold co-treated with icariin and calcium...
January 10, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29316874/liposomal-delivery-of-demineralised-dentine-matrix-for-dental-tissue-regeneration
#19
Genevieve Elizabeth Melling, John Colombo, Steven J Avery, Wayne Ayre, Samuel L Evans, Rachel Jane Waddington, Alastair James Sloan
Current dental restorations have short longevity, consequently there is a need for novel tissue engineering strategies that aim to regenerate the dentine-pulp complex. Dentine matrix contains a myriad of bioactive growth factors and extracellular matrix proteins associated with the recruitment, proliferation and differentiation of dental pulp progenitor cells. Here, we show that demineralised dentine matrix (DDM), from non-carious dentine, can be encapsulated into liposomes for delivery to dental tissue to promote regeneration...
January 9, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29303417/evaluation-of-detergent-free-and-detergent-based-methods-for-decellularization-of-murine-skin
#20
Ali Farrokhi, Mohammadreza Pakyari, Layla Nabai, Amir Pourghadiri, Ryan Hartwell, Reza B Jalili, Aziz Ghahary
Acute and chronic wounds contribute to increased morbidity and mortality in affected people and impose significant financial burdens on healthcare systems. For these challenging wounds, Acellular Dermal Matrices (ADMs) have been used as a biological wound coverage. Unlike engineered dermal matrices, ADMs are prepared through the removal of cells from skin, while preserving the extracellular matrix structure and function. In this study, our primary objective was to develop a detergent-free method of decellularization of the skin to mitigate chemical stress on matrix molecules...
January 5, 2018: Tissue Engineering. Part A
journal
journal
41884
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"