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Tissue Engineering. Part A

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https://www.readbyqxmd.com/read/29676959/correction-to-enhanced-osteogenic-behavior-of-adscs-produced-by-deproteinized-antler-cancellous-bone-and-evidence-for-involvement-of-erk-signaling-pathway-by-wei-j-xu-m-zhang-x-meng-s-wang-y-zhou-t-ma-q-han-b-wei-y-and-deng-x-tissue-eng-part-a-2015-21-11-12
#1
https://www.readbyqxmd.com/read/29676227/chemically-modified-mrna-modified-rna-application-for-treatment-of-achilles-tendon-defects
#2
Elmar Herbst, Florian Imhoff, Peter Föhr, Stefan Milz, Christian Plank, Carsten Rudolph, Günther Hasenpusch, Johannes Geiger, Manish Aneja, Karoline Groth, Stephan Vogt, Andreas Imhoff, Andreas Schmitt
Different regenerative medicine approaches for tendon healing exist. Recently, especially gene therapy gained popularity. However, potential mutagenic and immunologic effects might prevent from translation to clinical research. Chemically modified mRNA (cmRNA) might bypass these limitations of gene therapy. Therefore, the purpose of this study was to evaluate the early healing properties of Achilles tendon defects in rats treated with basic fibroblast growth factor (bFGF) cmRNA. Forty male Lewis rats were used for the study and randomly assigned to the two study groups: 1) treatment with cmRNA coding for bFGF, 2) non-coding cmRNA control...
April 20, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29676206/fabrication-of-vascularized-bone-flaps-with-sustained-release-of-rhbmp-2-and-arteriovenous-bundle
#3
Bo Li, Changshun Ruan, Yufei Ma, Zhifeng Huang, Zhenfei Huang, Gang Zhou, Jing Zhang, Hai Wang, Zhihong Wu, Guixing Qiu
INTRODUCTION: It is a common treatment strategy in the clinic to transplant a vascularized bone flap for a large bone defect. But it is difficult for peripheral blood vessels to grow into the central region of a large bone construct. In this study, we fabricated a vascularized bone flap from a 3D-printed biodegradable PLGA/β-TCP scaffold using the combination of an arteriovenous (AV) bundle and recombinant human bone morphogenetic protein-2 (rhBMP-2). MATERIALS AND METHODS: A degradable porous poly (lactide-co-glycolide) /-tri-calcium phosphate (PLGA/β-TCP) scaffold was prepared by adopting 3D plotting and a low-temperature deposition technique...
April 20, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29676203/-5p-and-3p-strands-of-mir-145-and-mir-140-during-mesenchymal-stem-cell-chondrogenic-differentiation
#4
Jonathan D Kenyon, Olga Sergeeva, Rodrigo A Somoza, Ming Li, Arnold I Caplan, Ahmad M Khalil, Zhenghong Lee
The chondrogenic differentiation of mesenchymal stem cells (MSCs) is mediated by transcription factors and small non-coding RNAs such as micro-RNAs (miRNAs). Each miRNA is initially transcribed as a long transcript, which matures to produce -5p and -3p strands. It is widely believed that the mature and functional miRNA from any given pre-miRNA, usually the -5p strand, is functional, while the opposing -3p strand is degraded. However, recent cartilage literature started to show functional -3p stands for a few miRNAs...
April 20, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29667532/human-osteoblast-derived-extracellular-matrix-with-high-homology-to-bone-proteome-is-osteopromotive
#5
Marta Baroncelli, Bram C J van der Eerden, Siddharth Chatterji, Enrique Rull Trinidad, Yik Y Kan, Marijke Koedam, Ingmar A J van Hengel, Rodrigo D A M Alves, Lidy E Fratila-Apachitei, Jeroen A A Demmers, Jeroen van de Peppel, Johannes Ptm van Leeuwen
Efficient osteogenic differentiation of mesenchymal stem cells (MSCs) is crucial to accelerate bone formation. In this context, the use of extracellular matrix (ECM) as natural 3D-framework mimicking in vivo tissue architecture is of interest. The aim of this study was to generate a devitalized human osteogenic MSC-derived ECM and to investigate its impact on MSC osteogenic differentiation to improve MSC properties in bone regeneration. The devitalized ECM significantly enhanced MSC adhesion and proliferation...
April 18, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29661055/messenger-rna-delivery-for-tissue-engineering-and-regenerative-medicine-applications
#6
Siddharth Patel, Avathamsa Athirasala, Paula Dos Passos Menezes, Narayanan Ashwanikumar, Ting Zou, Gaurav Sahay, Luiz Bertassoni
The ability to control cellular processes and precisely direct cellular reprogramming has revolutionized regenerative medicine. Recent advances in in vitro transcribed (IVT) mRNA technology with chemical modifications have led to development of methods that control spatio-temporal gene expression. Additionally, there is a current thrust towards the development of safe, integration-free approaches to gene therapy for translational purposes. In this review, we describe strategies of synthetic IVT mRNA modifications and non-viral technologies for intracellular delivery...
April 17, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29649957/tissue-engineered-neo-urinary-conduit-from-decellularized-trachea
#7
Anirudha Anirudhasingh Gmail Com Singh, David Lee, Harrison Jeong, Christine Yu, Jiuru Li, ChenHao Fang, Praveena Sabnekar, Xiaopu Liu, Takahiro Yoshida, Nikolai Sopko, Trinity Bivalacqua
Decellularized tissues have been increasingly popular for constructing scaffolds for tissue engineering applications due to their beneficial biological compositions and mechanical properties. It is therefore natural to consider decellularized trachea for construction of tissue-engineered trachea as well as other tubular organs. A neo-urinary conduit (NUC) is such a tubular organ that works as a passage for urine removal in bladder cancer patients who need a urinary diversion after their diseased bladder is removed...
April 13, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29644940/transient-scleraxis-overexpression-combined-with-cyclic-strain-enhances-ligamentous-differentiation
#8
Anne C Nichols, Stephen R Werre, Linda A Dahlgren
Efforts to generate tissue engineered anterior cruciate ligament replacements are limited by a lack of methods to derive mature ligament cells. Viral overexpression of the tendon/ligament marker scleraxis (Scx) can drive cell differentiation; however, the use of viral vectors hampers translation to clinical use. In this study, C3H10T1/2 cells were transiently transfected with expression vectors containing the full-length murine Scx cDNA and cultured in 3D collagen hydrogels under static or cyclic strain for up to 14 days...
April 12, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29644938/acceleration-of-diabetic-wound-healing-with-phd2-and-mir-210-targeting-oligonucleotides
#9
Anne Dallas, Artem Trotsyuk, Heini Ilves, Clark A Bonham, Melanie Rodrigues, Karl Engel, Janos A Barrera, Nina Kosaric, Zachary A Stern-Buchbinder, Aleksandr White, Kenneth J Mandell, Paula Hammond, Jonathan N Mansbridge, Sumedha Jayasena, Geoffrey C Gurtner, Brian H Johnston
[PLACEHOLDER].
April 12, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29318958/a-cell-engineered-small-intestinal-submucosa-based-bone-mimetic-construct-for-bone-regeneration
#10
Mei Li, Chi Zhang, Yuxing Mao, Yi Zhong, Jiyuan Zhao
Extracellular matrix (ECM)-ornamented biomaterials have attracted attention due to their high potential to improve the biofunctionality of original materials. It is thought that ECM with a bone mimetic microenvironment generated by the specific induction of osteoblasts would be more beneficial for bone regeneration than a regular ECM. In this study, we developed an osteogenic and mineralized ECM construct (Os/M-ECM-SIS) under the guidance of osteoblasts on a small intestinal submucosa (SIS) scaffold cotreated with icariin and calcium...
April 12, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29638193/maximizing-the-translational-yield-of-mrna-therapeutics-by-minimizing-5-utrs
#11
Zeljka Trepotec, Manish Aneja, Johannes Geiger, Günther Hasenpusch, Christian Plank, Carsten Rudolph
The 5'-untranslated region (5'-UTR) of mRNA contains structural elements, which are recognized by cell-specific RNA binding proteins thereby affecting the translation of the molecule. The activation of an innate immune response upon transfection of mRNA into cells is reduced when the mRNA comprises chemically modified nucleotides, putatively by altering the secondary structure of the molecule. Such alteration in the 5'-UTR in turn may affect the functionality of mRNA. Here we report on the impact of seven synthetic minimalistic 5'-UTR sequences on the translation of luciferase-encoding unmodified and different chemically modified mRNAs upon transfection in cell culture and in vivo...
April 11, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29580181/decellularised-cartilage-directs-chondrogenic-differentiation-creation-of-a-fracture-callus-mimetic
#12
Wollis Jude Vas, Mittal Shah, Thomas Stephen Blacker, Michael Roland Duchen, Paul Sibbons, Scott John Roberts
Complications that arise from impaired fracture healing have considerable socioeconomic implications. Current research in the field of bone tissue engineering predominantly aims to mimic the mature bone tissue microenvironment. This approach, however, may produce implants that are intrinsically unresponsive to the cues present during the initiation of fracture repair. As such, this study describes the development of decellularised xenogeneic hyaline cartilage matrix in an attempt to mimic the initial reparative phase of fracture repair...
March 27, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29580173/reinforced-electrospun-polycaprolactone-nanofibers-for-tracheal-repair-in-an-in-vivo-ovine-model
#13
Jakob M Townsend, Lindsey M Ott, Jean R Salash, Kar-Ming Fung, Jeremiah T Easley, Howard B Seim, Jed Johnson, Robert A Weatherly, Michael Scott Detamore
Tracheal stenosis caused by congenital anomalies, tumors, trauma, or intubation-related damage can cause severe breathing issues, diminishing the quality of life, and potentially becoming fatal. Current treatment methods include laryngotracheal reconstruction or slide tracheoplasty. Laryngotracheal reconstruction utilizes rib cartilage harvested from the patient, requiring a second surgical site. Slide tracheoplasty involves a complex surgical procedure to splay open the trachea and reconnect both segments to widen the lumen...
March 27, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29580168/photocrosslinkable-gelatin-tropoelastin-hydrogel-adhesives-for-peripheral-nerve-repair
#14
Jonathan R Soucy, Ehsan Shirzaei Sani, Roberto Portillo Lara, David Diaz, Felipe Dias, Anthony S Weiss, Abigail N Koppes, Ryan Koppes, Nasim Annabi
Suturing peripheral nerve transections is the predominant therapeutic strategy for nerve repair. However, the use of sutures lead to scar tissue formation, hinder nerve regeneration, and prevent functional recovery. Fibrin-based adhesives have been widely used for nerve reconstruction, but their limited adhesive and mechanical strength, and inability to promote nerve regeneration hampers their utility as a stand-alone intervention. To overcome these challenges, we engineered composite hydrogels that are neuro-supportive and possess strong tissue adhesion...
March 27, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29652610/combination-therapy-comprising-a-static-magnetic-field-with-contractility-improves-skin-wounds
#15
Byeong-Wook Song, Hyunki Hong, Yu Jin Jung, Ju Hyung Lee, Bong-Soo Kim, Hoon-Bum Lee
Cutaneous wounds can present significant clinical problems because of abnormal healing after deep dermal damage. Despite technical advances in wound care, there are still unmet needs that result from inefficient treatment. In this study, we aimed to improve skin wound healing using a contractibility band with static magnetic field (SMF), termed a Magnetic band (Mb). To examine the effect of the Mb on wound healing, full-thickness 15 mm × 35 mm excision wounds were surgically created on the dorsum of rats. An elastic and contractile band (non-treatment), or one neodymium magnet (Nd-1) or two magnets with an elastic and contractile band (Nd-2) were topically applied to the wound daily and the wound size was measured from day 1 to 7 after surgery...
March 21, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29652609/an-optimized-collagen-fibrin-blend-engineered-neural-tissue-promotes-peripheral-nerve-repair
#16
Christina Maria Anna Pia Schuh, Adam George Edward Day, Heinz Redl, James Phillips
Tissue engineering approaches in nerve regeneration often aim to improve results by bridging nerve defects with conduits that mimic key features of the nerve autograft. One such approach uses Schwann cell self-alignment and stabilisation within collagen gels to generate engineered neural tissue (EngNT). Here we investigated whether a novel blend of fibrin and collagen could be used to form EngNT, as prior to EngNT design a beneficial effect of fibrin on Schwann cell proliferation was observed. A range of blend formulations was tested in terms of mechanical behavior (gel formation, stabilisation, swelling, tensile strength and stiffness) and lead formulations were assessed in vitro...
March 21, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29562839/initial-i-in-vitro-i-development-of-a-potassium-based-intra-articular-injection-for-osteoarthritis
#17
Joshua Dean Erndt-Marino, Patricia Diaz-Rodriguez, Mariah Hahn
The long-term goal of this work is to develop a potassium (K<sup>+</sup>)-based intra-articular (IA) injection for osteoarthritis treatment. Within this context, the objectives of this study were to: 1) demonstrate that hyperosmolar K<sup>+</sup> solutions can suppress pro-inflammatory macrophage activation and 2) evaluate the therapeutic potential of a hyperosmolar K<sup>+</sup> solution relative to clinically utilized drug based IA injection (methyl-prednisolone acetate (MPA) - a corticosteroid) or a cell-based (human mesenchymal stem cell (hMSC)) injectable...
March 21, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29652608/immunomodulatory-role-of-stem-cell-from-human-exfoliated-deciduous-teeth-on-periodontal-regeneration
#18
Xianling Gao, Zongshan Shen, Meiliang Guan, Qiting Huang, Lingling Chen, Wei Qin, Xiaohu Ge, Haijia Chen, Yin Xiao, Zhengmei Lin
Periodontitis is initiated by the infection of periodontal bacteria and subsequent tissue inflammation due to immunoreaction, eventually leading to periodontal apparatus loss. Stem cells from human exfoliated deciduous teeth (SHEDs) have exhibited beneficial characteristics in dental tissue regeneration. However, the immunomodulatory functions of SHEDs have not been elucidated in the context of periodontitis treatment. In this study, we investigated the potential immunomodulatory effects of SHEDs on experimental periodontitis and demonstrated that multi-dose delivery of SHEDs led to periodontal tissue regeneration...
March 20, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29652607/encapsulation-of-mesenchymal-stem-cells-improves-vascularization-of-alginate-based-scaffolds
#19
Dominik Steiner, Lara Lingens, Laura Fischer, Katrin Köhn, Rainer Detsch, Aldo Roberto Boccaccini, Tobias Fey, Peter Greil, Christian Weis, Justus Beier, Raymund E Horch, Andreas Arkudas
Vascularization of bioartificial tissues can be significantly enhanced by the generation of an arterio-venous loop (AV loop). Besides the surgical vascularization, the choice of the scaffold and the applied cells are indispensable cofactors. The combination of alginate dialdehyde and gelatin (ADA-GEL) and mesenchymal stem cells (MSCs) is a promising approach with regards to biocompatibility, biodegradation as well as de novo tissue formation. In this study we targeted the investigation of the vascularization of ADA-GEL with and in absence of encapsulated MSCs in the AV loop model...
March 20, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29652606/development-of-pei-rank-sirna-complex-loaded-plga-nanocapsules-for-the-treatment-of-osteoporosis
#20
Deniz Sezlev Bilecen, Hasan Uludag, Vasif Hasirci
Osteoporosis, which is characterized by low bone mineral density and susceptibility to fracture, is caused by increased osteoclastic activity. Receptor Activator of Nuclear Factor Kappa B Ligand (RANKL)/RANK signaling plays an important role in osteoclast differentiation and activation. The current treatment strategies for osteoporosis do not directly address this underlying cause and generates undesired side effects. This led to emergence of controlled delivery systems to increase drug bioavailability and efficacy specifically at the bone tissue...
March 14, 2018: Tissue Engineering. Part A
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