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Tissue Engineering. Part A

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https://www.readbyqxmd.com/read/29032745/strategic-directions-in-biomimetics-and-osteoinduction
#1
Pamela Habibovic
Strategic Directions in Biomimetics and Osteoinduction.
October 15, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28826347/osteogenic-potential-of-tauroursodeoxycholic-acid-as-an-alternative-to-rhbmp-2-in-a-mouse-spinal-fusion-model
#2
Byoung Ju Kim, Yoshie Arai, Eun-Mi Park, Sunghyun Park, Alvin Bello, In-Bo Han, Soo-Hong Lee
The non-union rate after lumbar spinal fusion is potentially as high as 48%. To support efficient bone regeneration, recombinant human bone morphogenetic protein-2 (rhBMP-2) is commonly used as it is regarded as the most potent bone-inducing molecule. However, recently, there have been increasing concerns on the use of rhBMP-2 such as serious complications, including seroma and heterotopic ossification, and the low quality of bone at the center of fusion mass. Thus, many studies were conducted to find and to develop a potential alternative to rhBMP-2...
October 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28756737/mimicking-the-biochemical-and-mechanical-extracellular-environment-of-the-endochondral-ossification-process-to-enhance-the-in-vitro-mineralization-potential-of-human-mesenchymal-stem-cells
#3
Fiona E Freeman, Jessica Schiavi, Meadhbh A Brennan, Peter Owens, Pierre Layrolle, Laoise M McNamara
Chondrogenesis and mechanical stimulation of the cartilage template are essential for bone formation through the endochondral ossification process in vivo. Recent studies have demonstrated that in vitro regeneration strategies that mimic these aspects separately, either chondrogenesis or mechanical stimulation, can promote mineralization to a certain extent both in vitro and in vivo. However, to date no study has sought to incorporate both the formation of the cartilage template and the application of mechanical stimulation simultaneously to induce osteogenesis...
October 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28726579/the-immunomodulatory-role-of-bmp-2-on-macrophages-to-accelerate-osteogenesis
#4
Fei Wei, Yinghong Zhou, Jing Wang, Changsheng Liu, Yin Xiao
Bone morphogenetic protein-2 (BMP-2) is one of the most frequently used osteogenic factors for osteogenesis. Macrophages, acting as both immune cells and osteoclast precursors, have an indispensable functional input during multiple stages of bone healing. This study aims to investigate the immunoregulatory role of BMP-2 on macrophages and the subsequent effects on osteogenesis. In the subcutaneous implantation study, gelatin sponge incorporated with 20 μg/mL BMP-2 rendered significantly enhanced macrophage infiltration compared with the gelatin sponge control...
October 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29017397/liver-scaffolds-support-survival-and-metabolic-function-of-multilineage-neonatal-allogenic-cells
#5
Wessam Hassanein, Arielle Cimeno, Avraham Werdesheim, Bryan Buckingham, Joshua Harrison, Mehmet C Uluer, Ali Khalifeh, Carlos Rivera-Pratts, Stephen Klepfer, Jhade D Woodall, Urmil Dhru, Elliot Bromberg, Dawn Parsell, Cinthia Drachenberg, Rolf N Barth, John C LaMattina
Organ scaffold bioengineering is currently limited by the inability to effectively repopulate the scaffold with appropriately distributed functional cells. We examined the feasibility of a decellularized liver scaffold to support the growth and function of multilineage allogenic cells derived from either adult or neonatal liver cells. Cell slurries from neonatal and adult rat livers containing hepatocytes, cholangiocytes, and endothelial cells were introduced into decellularized adult rat liver scaffolds via the bile duct...
October 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28982297/articular-cartilage-repair-with-mesenchymal-stem-cells-after-chondrogenic-priming-a-pilot-study
#6
Troy Bornes, Adetola Adesida, Nadr Jomha
Bone marrow-derived mesenchymal stromal stem cells (BMSCs) are a promising cell source for treating articular cartilage defects. The objective of this study was to assess a protocol that involved autologous transplantation of BMSCs into full-thickness cartilage defects in sheep following isolation, expansion and a short period (4 days) of chondrogenic priming. The impact of oxygen tension during pre-implantation culture was investigated. It was hypothesized that chondrogenically primed BMSCs would produce superior cartilaginous repair tissue relative to control defects, and that culture under hypoxia would yield improved repair tissue in comparison to normoxia...
October 6, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28982292/promotion-of-myogenic-maturation-by-timely-application-of-electric-field-along-the-topographical-alignment
#7
Ung Hyun Ko, Suk-Hee Park, Hyunseung Bang, Mina Kim, Hyunjun Shin, Jennifer Shin
Engineered muscular substitutes can restore the impaired muscle functions when integrated properly into the host tissue. To generate functional muscles with sufficient contractility at the site of transplant, the in vitro construction of fully differentiated muscle fibers would be desired. Many previous reports have identified either topographical alignment or electrical stimulation as an effective tool to promote myogenic differentiation. However, optimization of spatial and temporal arrangement of these two physical cues for better differentiation and maturation of skeletal muscles has not been investigated...
October 6, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28978278/vascularization-of-the-arterio-venous-loop-in-a-rat-isolation-chamber-model-quantification-of-hypoxia-and-evaluation-of-its-effects
#8
Quan Yuan, Andreas Arkudas, Raymund E Horch, Matthias Hammon, Oliver Bleiziffer, Michael Uder, Hannes Seuss
Introduction The aim of this study was to analyze the three-dimensional distribution of hypoxia in the arterio-venous (AV) loop model in rats, by examining the distribution of hypoxia-inducible factor-1 alpha (HIF-1α). Materials and Methods AV loops were created from the femoral artery and vein of male Lewis rats and an interpositional graft from the contralateral femoral vein. This arteriovenous fistula was embedded in a fibrin-filled isolation chamber and subcutaneously implanted into the thigh. The specimens were harvested after 7 days (n=4), 10 days (n=5) and 14 days (n=4)...
October 5, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28978275/low-immunogenic-endothelial-cells-maintain-morphofunctional-properties-needed-for-tissue-engineering
#9
Skadi Lau, Dorothee Eicke, Marco Carvalho Oliveira, Bettina Wiegmann, Claudia Schrimpf, Axel Haverich, Rainer Blasczyk, Mathias Wilhelmi, Constanςa Figueiredo, Ulrike Böer
The limited availability of native vessels suitable for the application as hemodialysis shunts or bypass material demands new strategies in cardiovascular surgery. Tissue engineered vascular grafts containing autologous cells are considered as ideal vessel replacements due to the low risk of rejection. However, endothelial cells (EC), which are central components of natural blood vessels, are difficult to obtain from elderly patients of poor health. Umbilical cord blood represents a promising alternative source for EC but their allogeneic origin corresponds with the risk of rejection after allotransplantation...
October 5, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28978269/human-mesenchymal-stem-cells-pre-treated-with-il-1%C3%AE-and-stimulated-with-bmp-3-enhance-chondrogenesis
#10
Daphne Hingert, Helena Barreto Henriksson, Helena Brisby
BACKGROUND: Low back pain is one of the most common ailments in western countries afflicting more than 80% of the population and the main cause is considered to be degeneration of intervertebral discs (IVDs). IL-1β is a vital inflammatory cytokine found in abundance in degenerated disc environment whereas BMP-3 is believed to promote chondrogenesis through TGF-β pathway. AIM: The aim was to study the effects of BMP-3, IL-1β and combination (pre-treatment with IL-1β) on hMSCs encapsulated in PuraMatrix™ hydrogel (Phg) especially in the absence of TGF-β in order to investigate the proliferation, and differentiation ability of hMSCs over 28 days period...
October 5, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28826355/human-beta-defensin-2-and-3-mitigate-the-negative-effects-of-bacterial-contamination-on-bone-healing-in-rat-calvarial-defect
#11
Po-Hui Lee, Meng-Yu Chen, Yu-Lin Lai, Shyh-Yuan Lee, Hen-Li Chen
Bacterial contamination during the healing of bone defects frequently compromises the effects of bone regenerative therapy. Human beta-defensin-2 (hBD2) and -3 (hBD3) are antimicrobial peptides of human innate immune system with a broad antibacterial spectrum and rare bacterial resistance. The purpose of this study was to determine the effect of hBD2 and hBD3 on the healing of bacteria-contaminated bone defects. Rat bone marrow stromal cells (BMSCs) were infected with adenovirus to overexpress hBD2 or hBD3...
September 28, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28847204/the-future-of-tissue-engineering-and-regenerative-medicine-in-africa
#12
Kevin Dzobo, Dimakatso Alice Senthebane, Michael Pillay, Cornelius Ssemakalu, Nolutho Mkhumbeni, Keolebogile Shirley Caroline M Motaung
No abstract text is available yet for this article.
September 27, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28486025/efficacy-and-persistence-of-allogeneic-adipose-derived-mesenchymal-stem-cells-combined-with-hyaluronic-acid-in-osteoarthritis-after-intra-articular-injection-in-a-sheep-model
#13
Chenchen Feng, Xuan Luo, Na He, Huitang Xia, Xiaoteng Lv, Xue Zhang, Dan Li, Fei Wang, Jiaping He, Li Zhang, Xiangming Lin, Liping Lin, Huabin Yin, Jiyin He, Jingwu Wang, Wei Cao, Richard Wang, Guangdong Zhou, Wen Wang
Although a number of studies have reported efficacy of autologous adipose-derived mesenchymal stem cells (AD-MSCs) in treating osteoarthritis (OA) no reliable evidences demonstrate whether allogeneic AD-MSCs can efficiently block OA progression in a large animal model. This study explored the efficacy and survival of allogeneic AD-MSCs combined with hyaluronic acid (HA) after intra-articular (IA) injection in a sheep OA model, which were conventionally established by anterior cruciate ligament resection and medial meniscectomy...
September 27, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28793833/synergistic-therapeutic-effect-of-three-dimensional-stem-cell-clusters-and-angiopoietin-1-on-promoting-vascular-regeneration-in-ischemic-region
#14
Jung-Mi Kang, Jeong-Kee Yoon, Seong-Ja Oh, Byung-Soo Kim, Sang-Heon Kim
Peripheral artery disease (PAD) is an ischemic disease characterized by reduced blood flow to the legs, feet, and hands. Human mesenchymal stem cells are an attractive cell source to treat PAD in regenerative medicine. However, in clinical applications, the use of adult stem cells has several limitations, such as low cell viability and low therapeutic efficiency. In this study, we described an innovative method of culturing three-dimensional stem cell clusters (Angiocluster™ [AC]), demonstrated the potential for ACs to differentiate into vascular cells, and assessed the synergistic effects of ACs and angiopoietin-1 (Ang-1) on angiogenesis in ischemic animal models...
September 26, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28467735/skin-regeneration-with-self-assembled-peptide-hydrogels-conjugated-with-substance-p-in-a-diabetic-rat-model
#15
Ji Eun Kim, Jung Hwa Lee, Soo Hyun Kim, Youngmee Jung
The wound healing process requires enough blood to supply nutrients and various growth factors to the wound area. However, chronic wounds such as diabetic skin ulcers have limited regeneration due to a lack of cellular and molecular signals because of a deficient blood flow. Mesenchymal stem cells (MSCs) are known to provide various factors, including growth factors, cytokines, and angiogenic mediators. Although MSCs have great therapeutic potential, their transplantation has many obstacles, including the time required to culture the cells, the invasiveness of the procedure, and limited stem cell sources...
September 26, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28946828/retention-of-the-structure-and-function-of-heparan-sulfate-biomaterials-after-gamma-irradiation
#16
Raymond A A Smith, R J E Chua, Susan M Carnachan, Clarissa Ling Ling Tan, Ian M Sims, Simon F R Hinkley, Victor Nurcombe, Simon Cool
Heparan sulfate (HS) is a highly heterogeneous polysaccharide implicated in many important biological processes. Our previous work has demonstrated that a particular affinity-selected HS (referred to henceforth as 'HS3') is capable of enhancing the osteogenic effects of bone morphogenetic protein 2 (BMP2). Here, we gamma-irradiated HS with 26 kGy of ionizing radiation to determine how this affected the structure, composition and function. Initial structural studies were performed on a commercial preparation of HS as a proof-of-concept...
September 25, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28946792/calvarial-bone-regeneration-is-enhanced-by-sequential-delivery-of-fgf-2-and-bmp-2-from-layer-by-layer-coatings-with-a-biomimetic-calcium-phosphate-barrier-layer
#17
Gloria Gronowicz, Emily Jacobs, Tao Peng, Li Zhu, Marja Hurley, Liisa T Kuhn
A drug delivery coating for synthetic bone grafts has been developed to provide sequential delivery of multiple osteoinductive factors to better mimic aspects of the natural regenerative process. The coating is composed of a biomimetic calcium phosphate (bCaP) layer that is applied to a synthetic bone graft and then covered with a poly-L-Lysine/poly-L-Glutamic acid polyelectrolyte multilayer (PEM) film. Bone morphogenetic protein-2 (BMP-2) was applied prior to the coating process directly on the synthetic bone graft and then bCaP-PEM was deposited followed by adsorption of fibroblast growth factor-2 (FGF-2) into the PEM layer...
September 25, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28762866/in-vitro-bioactivity-of-one-and-two-dimensional-nanoparticle-incorporated-bone-tissue-engineering-scaffolds
#18
Jason T Rashkow, Gaurav Lalwani, Balaji Sitharaman
This study investigates the effect of incorporation of one- or two-dimensional nanoparticles with distinct composition and morphology on the bioactivity of biodegradable, biocompatible polymer matrices. 0.2 wt% multiwalled carbon nanotubes, multiwalled graphene nanoribbons, graphene oxide nanoplatelets (GONPs), molybdenum disulfide nanoplatelets (MSNPs), or tungsten disulfide nanotubes (WSNTs) were uniformly dispersed in poly(lactic-co-glycolic acid) (PLGA) polymer. PLGA or nanoparticle-incorporated PLGA were then incubated with simulated body fluid (SBF) under physiological conditions for 1, 3, 7, or 14 days...
September 25, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28726588/reconstituting-mouse-lungs-with-conditionally-reprogrammed-human-bronchial-epithelial-cells
#19
Ryan LaRanger, Jennifer R Peters-Hall, Melissa Coquelin, Busola R Alabi, Christopher T Chen, Woodring E Wright, Jerry W Shay
We developed methods for conditionally reprogramming (CR) primary human bronchial epithelial cells (HBECs) to extend their functional lifespan and permit their differentiation into both upper and lower airway lung epithelium. We also developed a bioreactor to support vascular perfusion and rhythmic breathing of decellularized mouse lungs reconstituted with CR HBECs isolated from patients with and without cystic fibrosis (CF). While conditionally reprogrammed cells only differentiate into an upper airway epithelium after 35 days at the air-liquid interface, in reconstituted lungs these cells differentiate into upper airway bronchial epithelium and lower airway alveolar structures after 12 days...
September 25, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28938862/rgdfk-peptide-modified-alginate-scaffold-for-cell-transplantation-and-cardiac-neovascularization
#20
Hugo Sondermeijer, Piotr Witkowski, Tetsunori Seki, Arnoud van der Laarse, Silviu Itescu, Mark Adam Hardy
Cell implantation for tissue repair is a promising new therapeutic strategy. Although direct injection of cells into tissue is appealing, cell viability and retention are not very good. Cell engraftment and survival following implantation are dependent on a sufficient supply of oxygen and nutrients via functional microcirculation as well as a suitable local microenvironment for implanted cells. Here, we describe the development of a porous, biocompatible, 3-dimensional alginate scaffold covalently modified with the synthetic cyclic RGDfK (Arg-Gly-Asp-D-Phe-Lys)-peptide...
September 22, 2017: Tissue Engineering. Part A
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