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Tissue Engineering. Part A

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https://www.readbyqxmd.com/read/28457188/functional-tissue-engineering-a-prevascularized-cardiac-muscle-construct-for-validating-human-mesenchymal-stem-cells-engraftment-potential-in-vitro
#1
Mani T Valarmathi, John W Fuseler, Jay D Potts, Jeffrey M Davis, Robert L Price
The influence of somatic stem cells in the stimulation of mammalian cardiac muscle regeneration is still in its early stages, and so far, it has been difficult to determine the efficacy of the procedures that have been employed. The outstanding question remains whether stem cells derived from the bone marrow or some other location within or outside of the heart can populate a region of myocardial damage and transform into tissue-specific differentiated progenies, and also exhibit functional synchronization...
May 25, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28537526/composite-biomaterial-as-a-carrier-for-bone-active-substances-for-metaphyseal-tibial-bone-defect-reconstruction-in-rats
#2
Peter Frederik Horstmann, Deepak Raina, Hanna Isaksson, Werner Hettwer, Lars Lidgren, Michael Mørk Petersen, Magnus Tägil
Restoring lost bone is a major challenge in orthopedic surgery. Currently available treatment-strategies all have shortcomings, like risk of infection, non-union and excessive resorption. Our primary aim was to study if a commercially available gentamicin-containing composite calcium sulphate/hydroxyapatite biomaterial (GBM) could serve as a carrier for local delivery of bone morphogenic protein-2 (BMP-2) and zoledronic acid (ZA) in a tibia defect-model in rats. Empty and allograft-filled defects were used as controls...
May 24, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28537502/suitability-of-different-natural-and-synthetic-biomaterials-for-dental-pulp-tissue-engineering
#3
Kerstin M Galler, Ferdinand Brandl, Susanne Kirchhof, Matthias Widbiller, Andreas Eidt, Wolfgang Buchalla, Achim Goepferich, Gottfried Schmalz
Dental pulp tissue engineering is possible after insertion of pulpal stem cells combined with a scaffold into empty root canals. Commonly used biomaterials are collagen or poly(lactic) acid, which are either difficult to modify or to insert into such a narrow space. New hydrogel scaffolds with bioactive, specifically tailored functions could optimize the conditions to engineer dental pulp. Different synthetic and natural hydrogels were tested for their suitability to engineer dental pulp. Two functionalized modifications of polyethylene glycol were developed in this study and compared to a self-assembling peptide as well as to collagen and fibrin...
May 24, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28537482/programmed-platelet-derived-growth-factor-%C3%AE-%C3%AE-and-bone-morphogenetic-protein-2-delivery-from-a-hybrid-calcium-phosphate-alginate-scaffold
#4
Emily A Bayer, Jahnelle Jordan, Riccardo Gottardi, Morgan Fedorchak, Prashant N Kumta, Steven Little
Bone tissue engineering requires the upregulation of several regenerative stages, including a critical early phase of angiogenesis. Previous studies have suggested that a sequential delivery of PDGF to BMP-2 could promote angiogenic tubule formation when delivered to in vitro co-cultures of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSCs). However, it was previously unclear that this PDGF to BMP-2 delivery schedule will result in cell migration into the scaffolding system and affect the later expression of bone markers...
May 24, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28463603/biomimetic-mineralization-of-biomaterials-using-simulated-body-fluids-for-bone-tissue-engineering-and-regenerative-medicine
#5
Kyungsup Shin, Timothy Acri, Sean Geary, Aliasger K Salem
Development of synthetic biomaterials imbued with inorganic and organic characteristics of natural bone that are capable of promoting effective bone tissue regeneration is an ongoing goal of regenerative medicine. Calcium phosphate (CaP) has been predominantly utilized to mimic the inorganic components of bone, such as calcium hydroxyapatite, due to its intrinsic bioactivity and osteoconductivity. CaP-based materials can be further engineered to promote osteoinductivity through the incorporation of osteogenic biomolecules...
May 22, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28530164/biocompatibility-of-intensified-decellularized-equine-carotid-arteries-in-a-rat-subcutaneous-implantation-model-and-in-a-human-in-vitro-model
#6
Niklas Jeinsen, Lavinia Maegel, Danny Jonigk, Melanie Klingenberg, Axel Haverich, Mathias Wilhelmi, Ulrike Böer
Limited biocompatibility of decellularized scaffolds is an ongoing challenge in tissue engineering. We recently demonstrated that intensified detergent-based decellularization of equine carotid artery (dEACintens) removed residual cellular molecules from the scaffold more efficiently than a conventional decellularization (dEACcon) though this approach did not eliminate its immunogenicity entirely. CCN1 has been shown to improve biocompatibility of dEACcon in a sheep model. Here, we tested the biocompatibility of dEACintens and dEACcon with or without CCN1-coating after subcutaneous implantation in rats for up to 12 weeks...
May 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28530157/muscle-secreted-factors-improve-anterior-cruciate-ligament-graft-healing-an-in-vitro-and-in-vivo-analysis
#7
Corina Adriana Ghebes, Nathalie Groen, Yau Chuk Cheuk, Sai Chuen Fu, Hugo Fernandes, Daniel B Saris
One of the ligaments most often damaged during sports - the anterior cruciate ligament (ACL) - has poor healing capacity. Upon damage, reconstructive surgery is performed to restore the mechanical stability of the knee and to reduce the inflammatory milieu otherwise present in the joint. A return to normal activities, however, takes between 9 and 12 months. Thus, strategies capable of improving ACL graft healing are needed. Embryonic development of tendon and ligament (T/L) is regulated by a crosstalk between different cell types...
May 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28530133/efficacy-of-a-self-assembling-peptide-hydrogel-spg-178-gel-for-bone-regeneration-and-three-dimensional-osteogenic-induction-of-dental-pulp-stem-cells
#8
Jun Tsukamoto, Keiko Naruse, Yusuke Nagai, Shuhei Kan, Nobuhisa Nakamura, Masaki Hata, Maiko Omi, Tatsuhide Hayashi, Tatsushi Kawai, Tatsuaki Matsubara
The aim of this study was to assess the efficacy of a self-assembling peptide hydrogel as a scaffold for bone regeneration. We used a neutral and injectable self-assembling peptide hydrogel, SPG-178-Gel. Bone defects (5 mm in diameter) in rat calvarial bones were filled with a mixture of alpha-modified Eagle's medium and the peptide hydrogel. Three weeks after surgery, soft X-ray and micro-computed tomography (micro-CT) images of the gel-treated bones showed new bone formations in the periphery and in central areas of the defects...
May 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28530131/a-novel-combination-of-a-polycaprolactone-fumarate-pclf-scaffold-with-polyethylene-terephthalate-pet-sutures-for-intra-articular-ligament-regeneration
#9
Joshua Parry, Eric R Wagner, Peter Kok, Mahrokh Dadsetan, Michael J Yaszemski, Andre J van Wijnen, Sanjeev Kakar
Intra-articular ligamentous injuries are typically unrepairable and have limited outcomes after graft reconstruction. A combination of porous polycaprolactone fumarate (PCLF) scaffolds with polyethylene terephthalate (PET) sutures was developed with the goal of regenerating intra-articular ligaments. Scaffolds were fabricated by injecting PCLF over 3D-printed molds containing two strands of PET suture down its central pore followed by crosslinking. Scaffolds were seeded with human mesenchymal stem cells (MSCs) from adipose tissue...
May 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28530130/injectable-graft-substitute-active-on-bone-tissue-regeneration
#10
Michela Bosetti, Alessia Borrone, Massimiliano Leigheb, Prasad Shastri, Mario Cannas
With the aim to obtain an injectable bioactive scaffold that can accelerate bone formation in sinus lift augmentation, in bony void and in fracture repair, we have developed a three-dimensional (3D) jelly collagen containing Lysophosphatidic acid (LPA) and 1α,25-Dihydroxyvitamin D3 (1,25D3). Using an in vitro 3D culture model of bone fracture we show that the contraction of the collagen gel is mediated by Rho-kinase activation in osteoblasts. This contraction was cell concentration dependent and was increased by LPA which favored apposition and fastening of bone fragments approach...
May 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28351289/bone-morphogenetic-protein-2-alters-osteogenesis-and-anti-inflammatory-profiles-of-mesenchymal-stem-cells-induced-by-microtextured-titanium-in-vitro
#11
Sharon L Hyzy, Rene Olivares-Navarrete, Sarah Ortman, Barbara D Boyan, Zvi Schwartz
OBJECTIVES: Microtextured titanium (Ti) induces osteoblast differentiation of mesenchymal stem cells (MSCs) in the absence of exogenous osteogenic factors; and high-energy surface modifications speed healing of microrough Ti implants. Bone morphogenetic protein 2 (BMP2) is used clinically to improve peri-implant bone formation and osseointegration but can cause inflammation and bone-related complications. In this study, we determined whether BMP2 alters human MSC differentiation, apoptosis, and inflammatory factor production when grown on Ti implants with different surface properties...
May 19, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28525313/a-tunable-three-dimensional-in-vitro-culture-model-of-growth-plate-cartilage-using-alginate-hydrogel-scaffolds
#12
Alek G Erickson, Taylor D Laughlin, Sarah M Romereim, Catherine N Sargus-Patino, Angela K Pannier, Andrew T Dudley
Defining the final size and geometry of engineered tissues through precise control of the scalar and vector components of tissue growth is a necessary benchmark for regenerative medicine, but it has proved to be a significant challenge for tissue engineers. The growth plate cartilage that promotes elongation of the long bones is a good model system for studying morphogenetic mechanisms because cartilage is composed of a single cell type, the chondrocyte; chondrocytes are readily maintained in culture; and growth trajectory is predominately in a single vector...
May 18, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28437177/periodontal-derived-mesenchymal-cell-sheets-promote-periodontal-regeneration-in-inflammatory-microenvironment
#13
Shujuan Guo, Jian Kang, Baohui Ji, Weihua Guo, Yi Ding, Yafei Wu, Weidong Tian
In this study, we investigated the periodontal regenerative potential of dental follicle cell (DFC) sheets and periodontal ligament cell (PDLC) sheets in the simulating inflammatory microenvironment of periodontitis, to confirm their regenerative potential for clinical application and explain the possible mechanism. The biological characteristics of DFC sheets and PDLC sheets were explored in lipopolysaccharide from Porphyromonas gingivalis (P. gingivalis LPS)-induced inflammation microenvironment in vitro, then cell sheets were transplanted into canine periodontal defects with experimental periodontitis in situ for 3 months...
May 18, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28351217/design-of-an-in-vitro-model-of-cell-recruitment-for-skeletal-muscle-regeneration-using-hepatocyte-growth-factor-loaded-fibrin-microthreads
#14
Jonathan M Grasman, Raymond L Page, George D Pins
Large skeletal muscle defects that result in volumetric muscle loss (VML) result in the destruction of the basal lamina, which removes key signaling molecules such as hepatocyte growth factor (HGF) from the wound site, eliminating the endogenous capacity of these injuries to regenerate. We recently showed that HGF-loaded fibrin microthreads increased the force production in muscle tissues after 60 days in a mouse VML model. In this study, we created an in vitro, three-dimensional (3D) microscale outgrowth assay system designed to mimic cell recruitment in vivo, and investigated the effect of HGF-loaded, cross-linked fibrin microthreads on myoblast recruitment to predict the results observed in vivo...
May 18, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28457190/exosome-like-vesicles-derived-from-adipose-tissue-provide-biochemical-cues-for-adipose-tissue-regeneration
#15
Minjia Dai, Mei Yu, Yan Zhang, Weidong Tian
There is an emerging need for soft tissue replacements in the field of reconstructive surgery for the treatment of congenital deformities, posttraumatic repair, and cancer rehabilitation. Previous studies have shown that the bioactive adipose tissue extract can induce adipogenesis without additional stem cells or growth factors. In this study, we innovatively investigated whether exosome-like vesicles derived from adipose tissue (ELV-AT) could direct stem cell differentiation and trigger adipose tissue regeneration...
May 17, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28457180/edc-cross-linking-of-decellularized-tissue-a-promising-approach
#16
Nadine Lehmann, Torsten Christ, Aila Daugs, Oliver Bloch, Sebastian Holinski
BACKGROUND: Decellularization of xenogenous cardiovascular structures is a promising approach to create scaffolds for tissue engineering. Unfortunately, handling and pliability of the unfixed tissue is challenging. N-(3-dimethylaminopropyl)-N9-ethylcarbodiimide (EDC) is an alternative cross-linking agent to glutaraldehyde (GA). Applied in native tissue, it provides biocompatibility and shows no potential for calcification. In addition, EDC can be used to link growth factors (GFs) to tissue scaffolds after decellularization...
May 15, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28498040/pivotal-role-of-non-cardiomyocytes-in-electromechanical-and-therapeutic-potential-of-induced-pluripotent-stem-cell-derived-engineered-cardiac-tissue
#17
Hiroko Iseoka, Shigeru Miyagawa, Satsuki Fukushima, Atsuhiro Saito, Shigeo Masuda, Shin Yajima, Emiko Ito, Nagako Sougawa, Maki Takeda, Akima Harada, Jong-Kook Lee, Yoshiki Sawa
Although engineered cardiac tissues (ECTs) derived from induced pluripotent stem cells (iPSCs) are promising for myocardial regenerative therapy, the appropriate ratio of cardiomyocytes to non-cardiomyocytes is not fully understood. Here, we determined whether ECT-cell content is a key determinant of its structure/function, thereby affecting ECT therapeutic potential for advanced heart failure. Scaffold-free ECTs containing different ratios (25%, 50%, 70%, or 90%) of iPSC-derived cardiomyocytes were generated by magnetic-activated cell sorting using cardiac-specific markers...
May 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28486045/in-vitro-generated-intervertebral-discs-towards-engineering-tissue-integration
#18
Jonathan Iu, Eric Massicotte, Shu-Qiu Li, Mark B Hurtig, Ehsan Toyserkani, J Paul Santerre, Rita Kandel
The intervertebral disc (IVD) is composed of nucleus pulposus (NP) surrounded by multilamellated annulus fibrosus (AF), and is located between the vertebral bodies. Current treatments for chronic neck or low back pain do not completely restore the functionality of degenerated IVDs. Thus developing biological disc replacements is an approach of great interest. Given the complex structure of the IVD, tissue engineering of the individual IVD components and then combining them together may be the only way to achieve this...
May 9, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28486025/efficacy-and-persistence-of-allogeneic-adipose-derived-mesenchymal-stem-cells-combined-with-hyaluronic-acid-in-osteoarthritis-after-intra-articular-injection-in-a-sheep-model
#19
Chenchen Feng, Xuan Luo, Na He, Huitang Xia, Xiaoteng Lv, Xue Zhang, Dan Li, Fei Wang, Jiaping He, Li Zhang, Xiangming Lin, Liping Lin, Huabin Yin, Jiyin He, Jinwu Wang, Wei Cao, Richard Wang, Guangdong Zhou, Wen Wang
Although a number of studies have reported efficacy of autologous adipose-derived mesenchymal stem cells (AD-MSCs) in treating osteoarthritis (OA), no reliable evidences demonstrate whether allogeneic AD-MSCs can efficiently block OA progression in a large animal model. The current study explored the efficacy and survival of allogeneic AD-MSCs combined with hyaluronic acid (HA) after intra-articular (IA) injection in a sheep OA model, which were conventionally established by anterior cruciate ligament resection and medial meniscectomy...
May 9, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28486019/role-of-bone-marrow-mononuclear-cell-seeding-for-nanofiber-vascular-grafts
#20
Takuma Fukunishi, Cameron A Best, Chin Siang Ong, Tyler Groehl, James Reinhardt, Tai Yi, Hideki Miyachi, Huaitao Zhang, Toshiharu Shinoka, Christopher K Breuer, Jed Johnson, Narutoshi Hibino
OBJECTIVE: Electrospinning is a promising technology that provides biodegradable nanofiber scaffolds for cardiovascular tissue engineering. However, success with these materials has been limited, and the optimal combination of scaffold parameters for a tissue-engineered vascular graft (TEVG) remains elusive. The purpose of the present study is to evaluate the effect of bone marrow mononuclear cell (BM-MNC) seeding in electrospun scaffolds to support the rational design of optimized TEVGs...
May 9, 2017: Tissue Engineering. Part A
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