We have located links that may give you full text access.
Nanoparticle Enhanced Radiotherapy to Trigger Pyrolysis and Anti-Tumor Immunity.
International Journal of Radiation Oncology, Biology, Physics 2021 November 2
PURPOSE/OBJECTIVE(S): Clinical evidence demonstrated that combination of radiotherapy and immunotherapy may be more effective than either treatment alone. However, the efficacy of combined treatment was limited due to the low response rate of immune checkpoints inhibit (ICIs). Pyrolysis is a lytic proinflammatory cell death mediated by the gasdermin family of pore-forming proteins. Studies indicated that combining radiotherapy with pyroptosis greatly enhance the body's anti-tumor immunity and radiotherapy efficacy. In order to precisely control the location and intensity of cell scorch, an accurate and efficient cancer immunotherapy system was constructed by organically combining radiotherapy and pyrolysis with nanomaterials with infrared light and pH responsive regulatory protein expression.
MATERIALS/METHODS: An upconverting nanoparticles with integrated azobenzene-modified mesoporous silica was constructed to realize the controlled release of the drug (decitabine (DAC)) under the control of infrared light. Firstly, synthesis of UCNPS@mSiO2 -azo. NaYF4: Tm, Yb@NaYF4 nanocrystals (UCNPS) was prepared by solvothermal method, and then UCNPS@mSiO2 was prepared by using cetyl trimethyl ammonium bromide (CTAB) as template and tetraethyl orthosilicate (TEOS) as silicon source. Finally, mesopores were formed by ion exchange method. UCNPS@mSiO2 -azo is prepared by mixing UCNP@mSiO2 and N-(3-triethoxysilyl) propyl-4-phenylazobenzamide in ethanol at 80°C for 1 hour. Secondly, Synthesis of UCNPS@mSiO2 -azo@DAC@HSA, (human serum albumin (HSA)). Disperse UCNPS@mSiO2 -azo in PBS, add a certain amount of DAC and stir for 24h, add the polymerized HSA and react at 37°C for 3h to obtain UCNPS@mSiO2 -azo@DAC@HSA.
RESULTS: The prepared UCNPS@mSiO2 with good up-conversion efficiency, which can convert infrared light into visible light and ultraviolet light (fig 1.a,c,d). At the same time, it was found that UCNPS@mSiO2 -azo can effectively load the DAC, and at the same time it has good stability, and can controllably release the DAC under the control of infrared light (fig 1.b). More importantly, it was found through cell experiments that the cells would not die after adding materials and performing infrared light. However, once further low-dose radiotherapy treatment, the cells would experience obvious pyrosis.
CONCLUSION: A specific and highly sensitive radiotherapy immunosensitization system that can effectively enhance the therapeutic effect of tumors was constructed. It can regulate the specific protein content of tumor location in vivo by means of both internal and external sources of pH and infrared light with the combination of radiotherapy. This work provides a new treatment strategy for radiotherapy by changing the way of cell death to enhance the body's anti-tumor immunity after local treatment to effectively reduce the damage of normal cells and to effectively change the tumor suppression microenvironment and enhance its own anti-tumor immunity to completely eliminate tumors.
MATERIALS/METHODS: An upconverting nanoparticles with integrated azobenzene-modified mesoporous silica was constructed to realize the controlled release of the drug (decitabine (DAC)) under the control of infrared light. Firstly, synthesis of UCNPS@mSiO2 -azo. NaYF4: Tm, Yb@NaYF4 nanocrystals (UCNPS) was prepared by solvothermal method, and then UCNPS@mSiO2 was prepared by using cetyl trimethyl ammonium bromide (CTAB) as template and tetraethyl orthosilicate (TEOS) as silicon source. Finally, mesopores were formed by ion exchange method. UCNPS@mSiO2 -azo is prepared by mixing UCNP@mSiO2 and N-(3-triethoxysilyl) propyl-4-phenylazobenzamide in ethanol at 80°C for 1 hour. Secondly, Synthesis of UCNPS@mSiO2 -azo@DAC@HSA, (human serum albumin (HSA)). Disperse UCNPS@mSiO2 -azo in PBS, add a certain amount of DAC and stir for 24h, add the polymerized HSA and react at 37°C for 3h to obtain UCNPS@mSiO2 -azo@DAC@HSA.
RESULTS: The prepared UCNPS@mSiO2 with good up-conversion efficiency, which can convert infrared light into visible light and ultraviolet light (fig 1.a,c,d). At the same time, it was found that UCNPS@mSiO2 -azo can effectively load the DAC, and at the same time it has good stability, and can controllably release the DAC under the control of infrared light (fig 1.b). More importantly, it was found through cell experiments that the cells would not die after adding materials and performing infrared light. However, once further low-dose radiotherapy treatment, the cells would experience obvious pyrosis.
CONCLUSION: A specific and highly sensitive radiotherapy immunosensitization system that can effectively enhance the therapeutic effect of tumors was constructed. It can regulate the specific protein content of tumor location in vivo by means of both internal and external sources of pH and infrared light with the combination of radiotherapy. This work provides a new treatment strategy for radiotherapy by changing the way of cell death to enhance the body's anti-tumor immunity after local treatment to effectively reduce the damage of normal cells and to effectively change the tumor suppression microenvironment and enhance its own anti-tumor immunity to completely eliminate tumors.
Full text links
Related Resources
Trending Papers
Revascularization Strategy in Myocardial Infarction with Multivessel Disease.Journal of Clinical Medicine 2024 March 27
Intravenous infusion of dexmedetomidine during the surgery to prevent postoperative delirium and postoperative cognitive dysfunction undergoing non-cardiac surgery: a meta-analysis of randomized controlled trials.European Journal of Medical Research 2024 April 19
The Tricuspid Valve: A Review of Pathology, Imaging, and Current Treatment Options: A Scientific Statement From the American Heart Association.Circulation 2024 April 26
Consensus Statement on Vitamin D Status Assessment and Supplementation: Whys, Whens, and Hows.Endocrine Reviews 2024 April 28
Management of Diverticulitis: A Review.JAMA Surgery 2024 April 18
Interstitial Lung Disease: A Review.JAMA 2024 April 23
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app