We have located links that may give you full text access.
MiRNA-137-mediated modulation of mitochondrial dynamics regulates human neural stem cell fate.
Stem Cells 2020 Februrary 4
The role of miRNAs in determining human neural stem cell fate remains elusive despite their high expression in the developing nervous system. In this study, we investigate the role of miR-137, a brain enriched miRNA, in determining the fate of human induced pluripotent stem cells (iPSCs)-derived neural stem cells (hiNSCs). We show that ectopic expression of miR-137 in hiNSCs reduces proliferation and accelerates neuronal differentiation and migration. TargetScan and MicroT-CDS predict myocyte enhancer factor-2A (MEF2A), a transcription factor that regulates peroxisome proliferator-activated receptor-gamma coactivator (PGC1α) transcription, as a target of miR-137. Using a reporter assay, we validate MEF2A as a downstream target of miR-137. Our results indicate that reduced levels of MEF2A reduce the transcription of PGC1α, which in turn impacts mitochondrial dynamics. Notably, miR-137 accelerates mitochondrial biogenesis in a PGC1α independent manner by upregulating NRF2 and TFAM. In addition, miR-137 modulates mitochondrial dynamics by inducing mitochondrial fusion and fission events, resulting in increased mitochondrial content and activation of oxidative phosphorylation (OXPHOS) and oxygen consumption rate (OCR). Pluripotency transcription factors OCT4 and SOX2 are known to have binding sites in the promoter region of miR-137 gene. Ectopic expression of miR-137 elevates the expression levels of OCT4 and SOX2 in hiNSCs which establishes a feed-forward self-regulatory loop between miR-137 and OCT4/SOX2. Our study provides novel molecular insights into NSC fate determination by miR-137. © AlphaMed Press 2020 SIGNIFICANCE STATEMENT: We describe the derivation of human neural stem cells (hNSCs) from human iPSCs that faithfully display immunoreactivity towards NSC markers and serve as a model to study neurological diseases. Using this model, we show that a brain enriched small non-coding RNA, miR-137, enhances neuronal differentiation by inducing mitochondrial biogenesis, fusion, fission and OXPHOS. Decrease in NSCs with age likely leads to compromised regenerative capacity of the brain. We propose that NSC differentiation induced by miR-137 may facilitate the design of treatments for aging-associated neurodegenerative diseases.
Full text links
Related Resources
Trending Papers
Renin-Angiotensin-Aldosterone System: From History to Practice of a Secular Topic.International Journal of Molecular Sciences 2024 April 5
Prevention and treatment of ischaemic and haemorrhagic stroke in people with diabetes mellitus: a focus on glucose control and comorbidities.Diabetologia 2024 April 17
British Society for Rheumatology guideline on management of adult and juvenile onset Sjögren disease.Rheumatology 2024 April 17
Albumin: a comprehensive review and practical guideline for clinical use.European Journal of Clinical Pharmacology 2024 April 13
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app