journal
MENU ▼
Read by QxMD icon Read
search

Stem Cells

journal
https://www.readbyqxmd.com/read/27925343/transplantation-of-thy1-cells-accelerates-liver-regeneration-by-enhancing-the-growth-of-small-hepatocyte-like-progenitor-cells-via-il17rb-signaling
#1
Norihisa Ichinohe, Masayuki Ishii, Naoki Tanimizu, Junko Kon, Yusuke Yoshioka, Takahiro Ochiya, Toru Mizuguchi, Koichi Hirata, Toshihiro Mitaka
Small hepatocyte-like progenitor cells (SHPCs) transiently form clusters in rat livers treated with retrorsine (Ret)/70% partial hepatectomy (PH). When Thy1(+) cells isolated from d-galactosamine-treated rat livers were transplanted into the livers of Ret/PH-treated rats, the mass of the recipient liver transiently increased during the first 30 days after transplantation, suggesting that liver regeneration was enhanced. Here we addressed how Thy1(+) cell transplantation stimulates liver regeneration. We found that the number and size of SHPC clusters increased in the liver at 14 days after transplantation...
December 7, 2016: Stem Cells
https://www.readbyqxmd.com/read/27888602/a-glycovariant-of-human-cd44-is-characteristically-expressed-on-human-mesenchymal-stem-cells
#2
Gisela Pachón-Peña, Conor Donnelly, Catalina Ruiz-Cañada, Adam Katz, Sonia Fernández-Veledo, Joan Vendrell, Robert Sackstein
The clinical effectiveness of systemically-administered human mesenchymal stem cells (hMSCs) depends on their capacity to engage vascular endothelium. hMSCs derived from bone marrow (BM-hMSCs) natively lack endothelial binding capacity but express a CD44 glycovariant containing N-linked sialyllactosamines that can be α(1,3)-fucosylated using fucosyltransferase- VI (FTVI) to enforce sLe(X) decorations, thereby creating hematopoietic cell E-/L-selectin ligand (HCELL). HCELL expression programs potent shear-resistant adhesion of circulating cells to endothelial beds expressing E-selectin...
November 26, 2016: Stem Cells
https://www.readbyqxmd.com/read/27888558/spontaneous-single-copy-loss-of-tp53-in-human-embryonic-stem-cells-markedly-increases-cell-proliferation-and-survival
#3
Hadar Amir, Thomas Touboul, Karen Sabatini, Divya Chhabra, Ibon Garitaonandia, Jeanne F Loring, Robert Morey, Louise C Laurent
Genomic aberrations have been identified in many hPSC cultures. Commonly observed duplications in portions of chromosomes 12p and 17q have been associated with increases in genetic instability and resistance to apoptosis, respectively. However, the phenotypic consequences related to sporadic mutations have not been evaluated to date. Here, we report on the effects of a single-copy deletion of the chr17p13.1 region, a sporadic mutation that spontaneously arose independently in several subclones of a hESC culture...
November 26, 2016: Stem Cells
https://www.readbyqxmd.com/read/27888550/mesenchymal-stem-stromal-cells-biology-and-preclinical-evidence-for-therapeutic-potential-for-organ-dysfunction-following-trauma-or-sepsis
#4
Michael A Matthay, Shibani Pati, Jae-Woo Lee
Several experimental studies have provided evidence that bone-marrow derived mesenchymal stem (stromal) cells (MSC) may be effective in treating critically ill surgical patients who develop traumatic brain injury, acute renal failure, or the acute respiratory distress syndrome. There is also preclinical evidence that MSC may be effective in treating sepsis-induced organ failure, including evidence that MSC have anti-microbial properties. This review considers preclinical studies with direct relevance to organ failure following trauma, sepsis or major infections that apply to critically ill patients...
November 26, 2016: Stem Cells
https://www.readbyqxmd.com/read/27882623/concise-review-stem-cell-based-treatment-of-pelizaeus-merzbacher-disease
#5
M Joana Osorio, David H Rowitch, Paul Tesar, Marius Wernig, Martha S Windrem, Steven A Goldman
Pelizaeus-Merzbacher disease (PMD) is an X-linked disorder caused by mutation in the proteolipid protein-1 (PLP1) gene, which encodes the proteolipid protein of myelinating oligodendroglia. PMD exhibits phenotypic variability that reflects its considerable genotypic heterogeneity, but all forms of the disease result in central hypomyelination, associated in most cases with early neurological dysfunction, progressive deterioration, and ultimately death. PMD may present as a connatal, classic and transitional forms, or as the less severe spastic paraplegia type 2 and PLP-null phenotypes...
November 23, 2016: Stem Cells
https://www.readbyqxmd.com/read/27873381/cutting-edge-advances-in-stem-cell-biology-and-therapy
#6
EDITORIAL
Jan A Nolta
No abstract text is available yet for this article.
November 22, 2016: Stem Cells
https://www.readbyqxmd.com/read/27870307/abrogation-of-gap-junctional-communication-in-es-cells-results-in-a-disruption-of-primitive-endoderm-formation-in-embryoid-bodies
#7
Philipp Wörsdörfer, Felicitas Bosen, Martina Gebhardt, Nicole Russ, Katrin Zimmermann, David Komla Kessie, Thileepan Sekaran, Angela Egert, Süleyman Ergün, Hubert Schorle, Alexander Pfeifer, Frank Edenhofer, Klaus Willecke
Gap junctional intercellular communication (GJIC) has been suggested to be involved in early embryonic development but the actual functional role remained elusive. Connexin (Cx) 43 and Cx45 are co-expressed in embryonic stem (ES) cells, form gap junctions and are considered to exhibit adhesive function and/or to contribute to the establishment of defined communication compartments. Here we describe the generation of Cx43/Cx45-double deficient mouse ES cells to achieve almost complete breakdown of GJIC. Cre-loxP induced deletion of both, Cx43 and Cx45, results in a block of differentiation in embryoid bodies without affecting pluripotency marker expression and proliferation in ES cells...
November 21, 2016: Stem Cells
https://www.readbyqxmd.com/read/27870267/thyroid-hormone-induced-activation-of-notch-signaling-is-required-for-adult-intestinal-stem-cell-development-during-xenopus-laevis-metamorphosis
#8
Takashi Hasebe, Kenta Fujimoto, Mitsuko Kajita, Liezhen Fu, Yun-Bo Shi, Atsuko Ishizuya-Oka
In Xenopus laevis intestine during metamorphosis, the larval epithelial cells are removed by apoptosis, and the adult epithelial stem (AE) cells appear concomitantly. They proliferate and differentiate to form the adult epithelium (Ep). Thyroid hormone (TH) is well established to trigger this remodeling by regulating the expression of various genes including Notch receptor. To study the role of Notch signaling, we have analyzed the expression of its components, including the ligands (DLL and Jag), receptor (Notch), and targets (Hairy), in the metamorphosing intestine by real-time reverse transcription-polymerase chain reaction and in situ hybridization or immunohistochemistry...
November 21, 2016: Stem Cells
https://www.readbyqxmd.com/read/27870251/getting-to-the-core-of-inherited-bone-marrow-failures
#9
Soheir Adam, Dario Melguizo Sanchis, Ghada El-Kamah, Sujith Samarasinghe, Sameer Alharthi, Lyle Armstrong, Majlinda Lako
Bone marrow failure syndromes (BMFS) are a group of disorders with complex pathophysiology characterized by a common phenotype of peripheral cytopenia and/or a hypoplastic bone marrow. Understanding genetic factors contributing to the pathophysiology of BMFS has enabled the identification of causative genes and development of diagnostic tests. To date more than 40 mutations in genes involved in maintenance of genomic stability, DNA repair, ribosome and telomere biology have been identified. In addition, pathophysiological studies have provided insights into several biological pathways leading to the characterization of genotype/phenotype correlations as well as the development of diagnostic approaches and management strategies...
November 21, 2016: Stem Cells
https://www.readbyqxmd.com/read/27870168/proteome-and-secretome-characterisation-of-glioblastoma-derived-neural-stem-cells
#10
Satoshi Okawa, Sladjana Gagrica, Carla Blin, Christine Ender, Steven M Pollard, Jeroen Krijgsveld
Glioblastoma multiforme (GBM) (grade IV astrocytoma) is the most common and aggressive primary brain tumour. GBM consists of heterogeneous cell types includinga subset of stem cell-like cells thought to sustain tumour growth. These tumour-initiating GBM-derived neural stem (GNS) cells as well as their genetically normal neural stem (NS) counterparts can be propagated in culture as relatively pure populations. Here we perform quantitative proteomics to globally characterize and compare total proteome plus the secreted proteome (secretome) between GNS cells and NS cells...
November 21, 2016: Stem Cells
https://www.readbyqxmd.com/read/27862694/mesenchymal-stem-cell-derived-microvesicles-modulate-lps-induced-inflammatory-responses-to-microglia-cells
#11
Yarúa Jaimes, Yahaira Naaldijk, Kerstin Wenk, Christiane Leovsky, Frank Emmrich
Microglia cells are the central nervous system immune cells and have been pointed out as the main mediators of the inflammation leading to neurodegenerative disorders. Mesenchymal stromal cells (MSCs) are a heterogeneous population of cells with very high self-renewal properties and uncomplicated in vitro culture. Research has shown that MSCs have the capacity to induce tissue regeneration and reduce inflammation. Studies demonstrated that MSCs have complex paracrine machineries involving shedding of cell-derived microvesicles (MVs), which entail part of the regulatory and regenerative activity of MSCs, as observed in animal models...
November 16, 2016: Stem Cells
https://www.readbyqxmd.com/read/27862634/expression-analysis-of-the-stem-cell-marker-pw1-peg3-reveals-a-cd34-negative-progenitor-population-in-the-hair-follicle
#12
Vanessa Besson, Sergiy Kyryachenko, Peggy Janich, Salvador A Benitah, Giovanna Marazzi, David Sassoon
Pw1/Peg3 is a parentally imprinted gene expressed in adult stem cells in every tissue thus far examined including the stem cells of the hair follicle. Using a Pw1/Peg3 reporter mouse, we carried out a detailed dissection of the stem cells in the bulge, which is a major stem cell compartment of the hair follicle in mammalian skin. We observed that PW1/Peg3 expression initiates upon placode formation during fetal development, coincident with the establishment of the bulge stem cells. In the adult, we observed that PW1/Peg3 expression is found in both CD34+ and CD34- populations of bulge stem cells...
November 15, 2016: Stem Cells
https://www.readbyqxmd.com/read/27862653/response-to-letter-to-the-editor-adenosine-producing-mesenchymal-stromal-cells
#13
Erja Kerkelä
Adenosine signaling has been described as one of the many immunosuppressive mechanisms of mesenchymal stromal cells (MSCs). Central players in adenosine signaling are the ectonucleotidases CD39 and CD73, which convert ADP/ATP to AMP and AMP to adenosine, respectively. CD73 is one of the three phenotypic markers of MSCs and thus, highly expressed on MSCs. Instead, there has been conflicting results whether MSCs intrinsically express CD39 or not. Stem Cells 2016.
November 11, 2016: Stem Cells
https://www.readbyqxmd.com/read/27860054/low-dose-pesticide-mixture-induces-senescence-in-normal-mesenchymal-stem-cells-msc-and-promotes-tumorigenic-phenotype-in-premalignant-msc
#14
Mazene Hochane, Valerie Trichet, Claire Pecqueur, Pierre Avril, Lisa Oliver, Jerome Denis, Régis Brion, Jérôme Amiaud, Alain Pineau, Philippe Naveilhan, Dominique Heymann, François M Vallette, Christophe Olivier
Humans are chronically exposed to multiple environmental pollutants such as pesticides with no significant evidence about the safety of such poly-exposures. We exposed mesenchymal stem cells (MSC) to very low doses of mixture of seven pesticides frequently detected in food samples for 21 days in vitro. We observed a permanent phenotype modification with a specific induction of an oxidative stress-related senescence. Pesticide mixture also induced a shift in MSC differentiation towards adipogenesis but did not initiate a tumorigenic transformation...
November 11, 2016: Stem Cells
https://www.readbyqxmd.com/read/27859962/concise-review-geminin-a-tale-of-two-tails-dna-replication-and-transcriptional-epigenetic-regulation-in-stem-cells
#15
Alexandra L Patmanidi, Spyridon Champeris Tsaniras, Dimitris Karamitros, Christina Kyrousi, Zoi Lygerou, Stavros Taraviras
Molecular mechanisms governing maintenance, commitment, and differentiation of stem cells are largely unexploited. Molecules involved in the regulation of multiple cellular processes are of particular importance for stem cell physiology, as they integrate different signals and coordinate cellular decisions related with self-renewal and fate determination. Geminin has emerged as a critical factor in DNA replication and stem cell differentiation in different stem cell populations. Its inhibitory interaction with Cdt1, a member of the prereplicative complex, ensures the controlled timing of DNA replication and, consequently, genomic stability in actively proliferating cells...
November 11, 2016: Stem Cells
https://www.readbyqxmd.com/read/27859896/adenosine-producing-mesenchymal-stem-cells
#16
LETTER
P J Schuler, S Brandau
Recent findings support the importance of the adenosine pathway in human immunology. Tissue specific differences exist with respect to the capacity of MSC to produce immune regulatory ATP metabolites. While some MSC may produce adenosine in a cell-autonomous fashion, other types of MSC require the cooperative activity of T-cells. This article is protected by copyright. All rights reserved.
November 11, 2016: Stem Cells
https://www.readbyqxmd.com/read/27862629/secreted-ectodomain-of-sialic-acid-binding-ig-like-lectin-9-and-mcp-1-synergistically-regenerate-transected-rat-peripheral-nerves-by-altering-macrophage-polarity
#17
Fumiya Kano, Kohki Matsubara, Minoru Ueda, Hideharu Hibi, Akihito Yamamoto
Peripheral nerves exhibit remarkable self-repairing reparative activity after a simple crush or cut injury. However, the neuronal transection involving a nerve gap overwhelms their repairing activity and causes persistent paralysis. Here, we show that an implantation of the serum-free conditioned medium from stem cells from human exfoliated deciduous teeth (SHED-CM) immersed in a collagen sponge into the nerve gap formed by rat facial nerves transection restored the neurological function. In contrast, SHED-CM specifically depleted of a set of anti-inflammatory M2 macrophage inducers, Monocyte monocyte chemoattractant protein-1 (MCP-1) and the secreted ectodomain of sialic acid-binding Ig-like lectin-9 (sSiglec-9) lost the ability to restore neurological function in this model...
November 10, 2016: Stem Cells
https://www.readbyqxmd.com/read/27862618/scleraxis-lineage-cells-contribute-to-ectopic-bone-formation-in-muscle-and-tendon
#18
Shailesh Agarwal, Shawn J Loder, David Cholok, Joshua Peterson, John Li, Christopher Breuler, R Cameron Brownley, Hsiao Hsin Sung, Mike Chung, Nobuhiro Kamiya, Shuli Li, Bin Zhao, Vesa Kaartinen, Thomas A Davis, Ammar T Qureshi, Ernestina Schipani, Yuji Mishina, Benjamin Levi
The pathologic development of heterotopic ossification (HO) is well described in patients with extensive trauma or with hyperactivating mutations of the bone morphogenetic protein (BMP) receptor ACVR1. However, identification of progenitor cells contributing to this process remains elusive. Here we show that connective tissue cells contribute to a substantial amount of HO anlagen caused by trauma using postnatal, tamoxifen-inducible, scleraxis-lineage restricted reporter mice (Scx-creERT2/tdTomato(fl/fl) )...
November 8, 2016: Stem Cells
https://www.readbyqxmd.com/read/27800660/treatment-of-severe-adult-traumatic-brain-injury-using-bone-marrow-mononuclear-cells
#19
Charles S Cox, Robert A Hetz, George P Liao, Benjamin M Aertker, Linda Ewing-Cobbs, Jenifer Juranek, Sean I Savitz, Margaret L Jackson, Anna M Romanowska-Pawliczek, Fabio Triolo, Pramod K Dash, Claudia Pedroza, Dean A Lee, Laura Worth, Imoigele P Aisiku, Huimahn A Choi, John B Holcomb, Ryan S Kitagawa
BACKGROUND: Numerous pre-clinical studies using bone marrow derived cells for the treatment of traumatic brain injury and stroke have demonstrated efficacy in terms of blood-brain barrier preservation, neurogenesis, and other functional outcomes. Phase 1 clinical trials using bone marrow mononuclear cells infused intravenously in children with severe TBI demonstrated safety and potentially a CNS structural preservation treatment effect. This study sought to confirm the safety, logistic feasibility, and potential treatment effect size of structural preservation/inflammatory biomarker mitigation in adults to guide Phase 2 clinical trial design...
November 1, 2016: Stem Cells
https://www.readbyqxmd.com/read/27790799/formyl-peptide-receptor-2-is-involved-in-cardiac-repair-after-myocardial-infarction-through-mobilization-of-circulating-angiogenic-cells
#20
Soon Chul Heo, Yang Woo Kwon, Il Ho Jang, Geun Ok Jeong, Tae Wook Lee, Jung Won Yoon, Ho Jin Shin, Hae Chang Jeong, Young Keun Ahn, Tae Hee Ko, Sang Chul Lee, Jin Han, Jae Ho Kim
Increasing evidence suggests that circulating angiogenic cells (CACs) promote repair of ischemic tissues. Activation of formyl peptide receptor 2 (Fpr2) has been reported to stimulate repair of ischemic heart. This study was conducted to investigate the role of Fpr2 on CAC mobilization and cardiac protection in myocardial infarction (MI). WKYMVm, a strong agonist for Fpr2, was administered in a murine model of acute MI, and mobilization of CACs including endothelial progenitor cells (CD34(+) Flk1(+) or Sca1(+) Flk1(+) cells) in peripheral blood was monitored...
October 28, 2016: Stem Cells
journal
journal
31287
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"