SLC30A8 Gene rs13266634 C/T Polymorphism in Type 1 Diabetes Children of Tamil Nadu, India.

OBJECTIVE: Zinc transporter 8 (ZnT8) is a multi-trans membrane protein situated in the insulin secretory granule of the islets of β-cells and is identified as a novel auto-antigen in Type 1 diabetes (T1D). The gene coding for ZnT8 (solute carrier family 30 member 8; SLC30A8) is located in Chromosome 8q24.11. In this work it is aimed to identify the association of SLC30A8 rs13266634 C/T gene polymorphism with T1D in chosen children of Tamil Nadu, India.

METHODS: The family based study is made on 121 T1D patients and 214 of their family members as control. The SLC30A8 gene rs13266634 C/T polymorphism is evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

RESULTS: No significant differences was observed in allele (OR=0.92; CI=0.33 - 2.58; p=0.88) and genotype (CC: p=0.74; CT: p=0.82; TT: p=0.80) frequencies of rs13266634 C/T between T1D patients and controls. Transmission disequilibrium test (TDT) has identified over-transmission of mutant T allele from parents to affected children (T: U = 9:7) without statistical significance. Metaanalysis on the overall effects of rs13266634 C allele frequency was not different (p=0.10 and P heterogeneity = 0.99) in T1D patients upon comparison with control.

CONCLUSION: The present study along with the meta-analysis does not show any substantial association of the rs13266634 C/T polymorphism with T1D development.