Huberine, a New Canthin-6-One Alkaloid from the Bark of Picrolemma huberi.

A new alkaloid, Canthin-6-one, Huberine ( 1 ), together with three known compounds including 1-Hydroxy-canthin-6-one ( 2 ), Canthin-6-one ( 3 ) and stigma sterol ( 4 ), were isolated from the stem bark of Picrolemma huberi . The isolation was achieved by chromatographic techniques and the purification was performed on a C18 column using acetonitrile/water (90:10, v / v ) with 0.1% formic acid as the mobile phase. The structural elucidation was performed via spectroscopic methods, notably 1D- and 2D-NMR, UV, IR, MS and HRMS. The antiplasmodial activity of the compounds was studied.