We have located links that may give you full text access.
Electrochemical reactive oxygen species detection by cytochrome c immobilized with vertically aligned and electrochemically reduced graphene oxide on a glassy carbon electrode.
Analyst 2017 November 21
A new amperometric biosensor for hydrogen peroxide (H2 O2 ) and superoxide anion (SO) has been developed. The biosensor developed uses cytochrome c (Cyt c) modified glassy carbon electrodes coupled with electrochemically reduced graphene oxide (ErGO). To immobilize Cyt c, the "one step" electrochemical deposition of vertically aligned and ErGO (VAErGO) has been performed by using a pulse reverse technique, thus resulting in a very simple and efficient system. The well-established vertical alignment of ErGO was confirmed by atomic force microscopy and the electrochemical characteristics of the biosensor were investigated by cyclic voltammetric, electrochemical impedance spectroscopy and amperometric techniques. The surface coverage (Γ) of immobilized Cyt c was effectively increased by the vertical alignment of ErGO and found to be 1.03 × 10-10 mol cm-2 . The direct electron transfer property of Cyt c was also improved by VAErGO and the heterogeneous electron transfer rate constant (ket ) was estimated to be 6.40 s-1 . To detect H2 O2 and SO, amperometric measurements were carried out at different operating potentials (0.0 V vs. Ag/AgCl for H2 O2 and +0.2 V for SO). The sensitivity and detection limit for H2 O2 were found to be 46.3 μA mM-1 cm-2 and 2.3 μM, and for SO were found to be 32.1 μM nA-1 cm-2 and 6.84 nM s-1 , respectively. Additionally, the designed biosensor exhibited strong anti-interference ability and satisfactory reproducibility.
Full text links
Related Resources
Trending Papers
Executive Summary: State-of-the-Art Review: Unintended Consequences: Risk of Opportunistic Infections Associated with Long-term Glucocorticoid Therapies in Adults.Clinical Infectious Diseases 2024 April 11
Clinical practice guidelines on the management of status epilepticus in adults: A systematic review.Epilepsia 2024 April 13
Autoimmune Hemolytic Anemias: Classifications, Pathophysiology, Diagnoses and Management.International Journal of Molecular Sciences 2024 April 13
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app