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Alexandra Aubé, Shirley Campbell, Andreea R Schmitzer, Audrey Claing, Jean-François Masson
Correction for 'Ultra-low fouling methylimidazolium modified surfaces for the detection of HER2 in breast cancer cell lysates' by Alexandra Aubé et al., Analyst, 2017, 142, 2343-2353.
July 27, 2017: Analyst
Feiyang Wang, Yuexiang Lu, Jiacheng Yang, Ying Chen, Wenjie Jing, Liuying He, Yueying Liu
A simple, visible and smartphone readable strategy has been proposed for the sensitive detection and discrimination of multiple proteins. By employing five different concentrations of NaCl salt, AuNP exhibited different aggregation behavior for different proteins because of differential ion strength, leading to diverse color changes. The sensing system could not only discriminate twelve proteins at the concentration of 50 nM in aqueous solution, but it could also discriminate these proteins at 200 nM in the presence of human urine with an accuracy of 100%...
July 26, 2017: Analyst
Heidi R Culver, Ishna Sharma, Marissa E Wechsler, Eric V Anslyn, Nicholas A Peppas
Due to the high cost and environmental instability of antibodies, there is precedent for developing synthetic molecular recognition agents for use in diagnostic sensors. While these materials typically have lower specificity than antibodies, their cross-reactivity makes them excellent candidates for use in differential sensing routines. In the current work, we design a set of charge-containing poly(N-isopropylacrylamide) (PNIPAM) nanogels for use as differential protein receptors in a turbidimetric sensor array...
July 26, 2017: Analyst
Karla Pelivan, Lisa Frensemeier, Uwe Karst, Gunda Koellensperger, Bjoern Bielec, Sonja Hager, Petra Heffeter, Bernhard K Keppler, Christian R Kowol
α-N-Heterocyclic thiosemicarbazones are among the most promising ribonucleotide reductase inhibitors identified so far. Triapine, the most prominent representative of this class of substances, has been investigated in multiple phase I and II clinical trials. With regard to clinical practice, Triapine showed activity against hematological diseases, but ineffectiveness against a variety of solid tumors. However, the reasons are still vague and the amount of ADME (absorption, distribution, metabolism and excretion) data for Triapine available in the literature is very limited...
July 26, 2017: Analyst
R Elashnikov, D Mares, T Podzimek, V Švorčík, O Lyutakov
A smart plasmonic sensor, comprising a layer of a stimuli-responsive polymer sandwiched between two gold layers, is reported. As a stimuli-responsive material, a monolayer of poly(N-isopropylacrylamide) (PNIPAm) crosslinked globules is used. A quasi-periodic structure of the top gold layer facilitates efficient excitation and serves as a support for plasmon excitation and propagation. The intermediate layer of PNIPAm efficiently entraps targeted molecules from solutions. The sensor structure was optimized for efficient light focusing in the "active" PNIPAm layer...
July 25, 2017: Analyst
Yan Qi, Liying Qiu, Wenjiao Fan, Chenghui Liu, Zhengping Li
A versatile flow cytometric bead assay (FCBA) coupled with a completely enzyme-free signal amplification mechanism is developed for the sensitive detection of microRNAs (miRNAs). This new strategy integrates click chemistry-mediated ligation chain reaction (CLCR) with hybridization chain reaction (HCR) for enzyme-free signal amplification on magnetic beads (MBs), and a flow cytometer for the robust fluorescence readout of the MBs. Firstly, target miRNA can initiate CLCR on the surface of MBs based on the click chemical ligation between dibenzocyclooctyne (DBCO)- and azide-modified single-stranded DNA (ssDNA) probes, and the amount of ligated ssDNA sequences on the MBs will be proportional to the dosage of target miRNA...
July 24, 2017: Analyst
Konstantin Usachev, Yoshiki Yamaguchi, Masayuki Takamatsu, Nataliya Pavlova, Vladimir Klochkov, Almira Kurbangalieva, Takefumi Murase, Taiji Shimoda, Katsunori Tanaka
In the present Communication, we propose a quite simple but previously overlooked approach for conveniently analyzing, assigning, and extracting sialic acid-containing N-glycan structures using high-resolution NMR spectroscopy without pre-installing metal chelators. Paramagnetic metals, such as Gd(3+), appear to bind to the carboxyl groups of N-acetylneuraminic acid when introduced at room temperature, leading to the measurement of nonequivalent proton and carbon NMR spectral signals among otherwise "identical" glycan branched structures...
July 21, 2017: Analyst
Ruochong Zhang, Fei Gao, Xiaohua Feng, Siyu Liu, Rahul Kishor, Yunqi Luo, Yuanjin Zheng
We proposed a novel noninvasive method for glucose detection using a photoacoustic (PA) technique utilizing both the peak-to-peak value and peak arrival time delay information. Without increasing apparatus and system complexity, we demonstrated the accuracy enhancement of 29.5% and 33.63% for high (0-7 g dL(-1)) and low (0-350 mg dL(-1)) concentration of glucose solution, compared to conventional amplitude-based prediction.
July 20, 2017: Analyst
Pengfei Cai, Datong Wu, Xiaoyong Zhao, Yuanjiang Pan
A novel task-specific ionic liquid derived from l-phenylalaninol was prepared as an enantioselective fluorescent sensor for the first time. Fluorescent chiral ionic liquid 1 (FCIL1) is found to exhibit highly enantioselective fluorescence enhancements toward both aromatic and non-aromatic chiral amino alcohols. When (S)-FCIL1 was treated with the enantiomers of phenylalaninol, a great fluorescence enhancement at 349 nm could be observed and the value of the enantiomeric fluorescence difference (ef) is 5.92...
July 20, 2017: Analyst
Inês P Santos, Elisa M Barroso, Tom C Bakker Schut, Peter J Caspers, Cornelia G F van Lanschot, Da-Hye Choi, Martine F van der Kamp, Roeland W H Smits, Remco van Doorn, Rob M Verdijk, Vincent Noordhoek Hegt, Jan H von der Thüsen, Carolien H M van Deurzen, Linetta B Koppert, Geert J L H van Leenders, Patricia C Ewing-Graham, Helena C van Doorn, Clemens M F Dirven, Martijn B Busstra, Jose Hardillo, Aniel Sewnaik, Ivo Ten Hove, Hetty Mast, Dominiek A Monserez, Cees Meeuwis, Tamar Nijsten, Eppo B Wolvius, Robert J Baatenburg de Jong, Gerwin J Puppels, Senada Koljenović
Oncological applications of Raman spectroscopy have been contemplated, pursued, and developed at academic level for at least 25 years. Published studies aim to detect pre-malignant lesions, detect cancer in less invasive stages, reduce the number of unnecessary biopsies and guide surgery towards the complete removal of the tumour with adequate tumour resection margins. This review summarizes actual clinical needs in oncology that can be addressed by spontaneous Raman spectroscopy and it provides an overview over the results that have been published between 2007 and 2017...
July 20, 2017: Analyst
Wentao Deng, Sarah L McLaughlin, David J Klinke
Modeling metastasis in vivo with animals is a priority for both revealing mechanisms of tumor dissemination and developing therapeutic methods. While conventional intravenous injection of tumor cells provides an efficient and consistent system for studying tumor cell extravasation and colonization, studying spontaneous metastasis derived from orthotopic tumor sites has the advantage of modeling more aspects of the metastatic cascade, but is challenging as it is difficult to detect small numbers of metastatic cells...
July 20, 2017: Analyst
Binfeng Yin, Wenshu Zheng, Mingling Dong, Wenbo Yu, Yiping Chen, Sang Woo Joo, Xingyu Jiang
We developed a competitive colorimetric nanosensor based on Au@Ag bimetallic nanoparticles for the detection of interleukin-6 (IL-6) in clinical samples. Antibody-conjugated magnetic nanoparticles (MNPs) and polystyrene (PS) microparticles conjugated with both catalase and a secondary antibody can form sandwich structures that enable one-step target enrichment and separation. The catalase on the surface of the PS can catalyze the hydrolysis of hydrogen peroxide (H2O2) to regulate the deposition of Ag(+) on the surface of gold nanoparticles (AuNPs), and forms different sizes and amounts of Au@Ag bimetallic nanoparticles (Au@AgNPs) which produce a distinct color signal for readout with the naked eye...
July 20, 2017: Analyst
Germán Augusto Gómez-Ríos, Tijana Vasiljevic, Emanuela Gionfriddo, Miao Yu, Janusz Pawliszyn
On-site screening for target analytes in complex matrices, such as biofluids and food specimens, not only requires reliable and portable analytical instrumentation, but also solvent-free and easy-to-use sampling/sample preparation approaches that allow analytes of interest to be isolated from such matrices. The integration of sampling devices with field deployable instruments should be as efficient as possible, and should aim to provide rapid, precise, and accurate results that enable quick on-site decision...
July 19, 2017: Analyst
Jiamin Gao, Cheng Zhao, Zhuomin Zhang, Gongke Li
Reliable quantification by surface enhanced Raman scattering (SERS) highly depends on the development of a reproducible substrate with excellent anti-interference capability. In this work, an intrinsic internal standard (IS) SERS substrate based on a diblock copolymer, polystyrene-block-poly-4-vinylpyridine (PS-b-P4VP), as a template was developed by a modified block copolymer lithography (BCL) technique of reserving the diblock copolymer template. This substrate generated a stable vibration peak at 1066 cm(-1) attributed to poly(4-vinylpyridine) (P4VP) which could be used as an intrinsic IS for precise SERS quantification...
July 19, 2017: Analyst
Hang Li, Akos Vertes
Most electrospray based ambient ionization techniques, e.g., laser ablation electrospray ionization (LAESI), utilize a fixed spray solution composition. Complex samples often contain compounds of different polarity that exhibit a wide range of solubilities in the electrospray solvent. Thus, the fixed spray solution composition limits the molecular coverage of these approaches. Two-barrel theta glass capillaries have been used for the rapid mixing of two solutions for manipulating fast reactions including protein folding, unfolding, and charge state distributions...
July 18, 2017: Analyst
Caddy N Hobbs, Justin A Johnson, Matthew D Verber, R Mark Wightman
Brain tissue injury is often accompanied by spreading depolarization (SD) events, marked by widespread cellular depolarization and cessation of neuronal firing. SD recruits viable tissue into the lesion, making it a focus for intervention. During SD, drastic fluctuations occur in ion gradients, extracellular neurotransmitter concentrations, cellular metabolism, and cerebral blood flow. Measuring SD requires a multimodal approach to capture the array of changes. However, the use of multiple sensors can inflict tissue damage...
July 17, 2017: Analyst
Graciela M Escandar, Alejandro C Olivieri
A large number of experimental applications of multi-way calibration are known, and a variety of chemometric models are available for the processing of multi-way data. While the main focus has been directed towards three-way data, due to the availability of various instrumental matrix measurements, a growing number of reports are being produced on order signals of increasing complexity. The purpose of this review is to present a general scheme for selecting the appropriate data processing model, according to the properties exhibited by the multi-way data...
July 17, 2017: Analyst
Kerene A Brown, Derek J Wilson
Hydrogen Deuterium Exchange (HDX) Mass Spectrometry (MS) is a sensitive analytical technique that provides information on protein conformation and dynamics in solution. It is commonly used in the study of protein-ligand and protein-protein interactions and more recently in the pharmaceutical industry for epitope mapping, screening drug candidates and in the comparison of biopharmaceuticals to biosimilars. HDX-MS monitors the exchange of protein backbone hydrogen atoms with deuterium in solution. Recent advancements in HDX automation and data analysis, have taken the emphasis off developing a fundamental understanding of HDX, which is still lacking...
July 13, 2017: Analyst
Debora Bonvin, Diego Chiappe, Marc Moniatte, Heinrich Hofmann, Marijana Mionić Ebersold
Nanoparticles (NPs) in contact with a biological environment get covered by proteins and some are loosely bound and some are tightly bound. The latter form a hard protein corona (HPC) which is known to determine their biological behavior. Therefore, in order to study the biological behaviour of NPs one needs to start from the HPC. However, established methods and standards of HPC isolation are still not known. This is especially a challenge in the case of magnetic NPs which form a major branch of nanomedicine...
July 11, 2017: Analyst
Jarod A Fincher, Andrew R Korte, Brent Reschke, Nicholas J Morris, Matthew J Powell, Akos Vertes
Laser ablation electrospray ionization-mass spectrometry (LAESI-MS) allows for direct analysis of biological tissues at atmospheric pressure with minimal to no sample preparation. In LAESI, a mid-IR laser beam (λ = 2.94 μm) is focused onto the sample to produce an ablation plume that is intercepted and ionized by an electrospray at the inlet of the mass spectrometer. In the remote LAESI platform, the ablation process is removed from the mass spectrometer inlet and takes place in an ablation chamber, allowing for incorporation of additional optics for microscopic imaging and targeting of specific features of the sample for laser ablation sampling...
July 5, 2017: Analyst
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