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Gabriel Antonio S Minero, Jeppe Fock, John S McCaskill, Mikkel F Hansen
We report on optomagnetic dose-dependent detection of DNA triplex-mediated and pH-switchable clusters of functionalised magnetic nanoparticles.
January 11, 2017: Analyst
G Diserens, D Hertig, M Vermathen, B Legeza, C E Flück, J M Nuoffer, P Vermathen
NMR measurements for metabolic characterization of biological samples like cells, biopsies or plasma, may take several hours for advanced methods. Preanalytical issues, such as sample preparation and stability over the measurement time, may have a high impact on metabolite content, and potentially lead to misinterpretation. The aim of this study was therefore to investigate by (1)H HR-MAS NMR the impact of different cell handling preparation protocols on the stability of the cell metabolite content over the measurement time...
January 11, 2017: Analyst
Franck Bonnier, Hélène Blasco, Clément Wasselet, Guillaume Brachet, Renaud Respaud, Luis Felipe C S Carvalho, Dominique Bertrand, Matthew J Baker, Hugh J Byrne, Igor Chourpa
Infrared spectroscopy is a reliable, rapid and cost effective characterisation technique, delivering a molecular finger print of the sample. It is expected that its sensitivity would enable detection of small chemical variations in biological samples associated with disease. ATR-IR is particularly suitable for liquid sample analysis and, although air drying is commonly performed before data collection, just a drop of human serum is enough for screening and early diagnosis. However, the dynamic range of constituent biochemical concentrations in the serum composition remains a limiting factor to the reliability of the technique...
January 9, 2017: Analyst
Jun-Kyu Park, Aaron Park, Si Kyung Yang, Sung-June Baek, Joonki Hwang, Jaebum Choo
In this paper, we consider a novel method for identification of Raman spectra recorded on different instruments with different wavelengths. Since the conventional hit quality index (HQI) is vulnerable to intensity variation, it needs intensity calibration or standardization for each spectrometer, which causes additional time consuming work. To simplify this process and enhance the identification performance, we propose a new scoring method which is defined as the weighted sum of the HQIs from segmented spectra by windowing...
January 9, 2017: Analyst
Hua Zhang, Qing Wang, Xiaohai Yang, Kemin Wang, Qing Li, Zhiping Li, Lei Gao, Wenyan Nie, Yan Zheng
A novel isothermal electrochemical biosensor was proposed for the sensitive detection of microRNA (miRNA) based on the ingenious combination of the target-catalyzed hairpin assembly (CHA) and supersandwich amplification strategies. Since miRNA-221 has been reported to be overexpressed in cancers and has been a potentially useful biomarker for the diagnosis of the related diseases, miRNA-221 was chosen as a model target miRNA. The target miRNA-221 triggered a toehold strand displacement assembly of the two hairpin substrates, which led to the cyclicality of the target miRNA and the CHA products...
December 23, 2016: Analyst
Tao Xie, Chao Jing, Yi-Tao Long
Plasmonic nanoparticles, especially Au and Ag, exhibit unique and tuneable optical properties due to their localized surface plasmon resonance, which have been widely applied in cell imaging, biocatalysis and biosensing. Notably, dark-field microscopy enables the observation of the scattering spectra of single nanoparticles and therefore has been intensively used in the construction of various nanosensors. In this mini review, we will briefly introduce the recent applications of plasmonic nanoparticles based on dark-field microscopy...
December 22, 2016: Analyst
Fabrício Augusto de Lima, Cyril Gobinet, Ganesh Sockalingum, Sérgio Britto Garcia, Michel Manfait, Valérie Untereiner, Olivier Piot, Luciano Bachmann
This paper presents a procedure that digitally neutralizes the contribution of paraffin to FTIR hyperspectral images. A brief mathematical derivation of the procedure is demonstrated and applied on one normal human colon sample to exemplify the de-waxing procedure. The proposed method includes construction of a paraffin model based on PCA, EMSC normalization and application of two techniques for spectral quality control. We discuss every step in which the researcher needs to take a subjective decision during the de-waxing procedure, and we explain how to make an adequate choice of parameters involved...
December 21, 2016: Analyst
Dinesh K R Medipally, Adrian Maguire, Jane Bryant, John Armstrong, Mary Dunne, Marie Finn, Fiona M Lyng, Aidan D Meade
Extensive research has been undertaken on the examination of tissue biopsies using vibrational spectroscopic techniques. However, fewer studies have focused on less invasive and commonly acquired blood samples. Recent studies have shown the ability of Raman and Fourier transform infrared (FTIR) spectroscopy to discriminate between non-cancer controls and cancer cases using blood serum or plasma. Even though many studies have proposed Raman spectroscopy as a potential diagnostic tool in various cancers, the Raman spectroscopic technique has not been introduced as a routine clinical technology...
December 21, 2016: Analyst
Adeline Huiling Loo, Chun Kiang Chua, Martin Pumera
3D printing, an upcoming technology, has vast potential to transform conventional fabrication processes due to the numerous improvements it can offer to the current methods. To date, the employment of 3D printing technology has been examined for applications in the fields of engineering, manufacturing and biological sciences. In this study, we examined the potential of adopting 3D printing technology for a novel application, electrochemical DNA biosensing. Metal 3D printing was utilized to construct helical-shaped stainless steel electrodes which functioned as a transducing platform for the detection of DNA hybridization...
December 21, 2016: Analyst
Yang Jun Kang
Correction for 'Continuous and simultaneous measurement of the biophysical properties of blood in a microfluidic environment' by Yang Jun Kang, Analyst, 2016, 141, 6583-6597.
December 20, 2016: Analyst
Gabriela B Almeida, Ronei J Poppi, José A Fracassi da Silva
Dielectrophoresis (DEP) is the electrokinetic movement of non-charged particles when they are subjected to a non-uniform electric field. This is a growing area of research, which can be used for trapping, concentrating and separating different particles. Some work has been reported with the intention of trapping metal particles to optimize the surface enhanced Raman spectroscopy (SERS) effect. In this paper, we use DEP with insulating structures (iDEP) to generate a non-uniform electric field for trapping gold nanoparticles (AuNP)...
December 20, 2016: Analyst
Hillary S Sloane, Morgan B Carter, Alexa E C Cecil, Delphine Le Roux, Daniel L Mills, James P Landers
Warfarin, a commonly prescribed oral anticoagulant, is burdened by a narrow therapeutic index and high inter-individual variability in response, making it the second leading cause of drug-related emergency room visits. Since genetic factors contribute significantly to warfarin sensitivity, a genotype-guided dosing strategy may reduce the occurrence of adverse events. While numerous methods have been demonstrated for warfarin genotyping, the specifications of most assays with respect to turnaround time and cost are not ideal for routine testing...
December 19, 2016: Analyst
Tanja Dučić, Tatjana Paunesku, Si Chen, Milena Ninković, Swetlana Speling, Charlene Wilke, Barry Lai, Gayle Woloschak
The glioblastoma (GBM) is characterized by a short median survival and an almost 100% tumor related mortality. GBM cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores application of X-ray and visible light microscopy to display the elemental and structural images of cells from 3 patient derived GMB samples and an established GMB cell line. Slight differences in elemental concentrations, in actin cytoskeleton organization and cell morphology were noted between all cells types by X-ray fluorescence and full field soft X-ray microscopy, as well as the Structured Illumination Super-resolution Microscope (SIM)...
December 16, 2016: Analyst
Christian G Kirchler, Cornelia K Pezzei, Krzysztof B Beć, Sophia Mayr, Mika Ishigaki, Yukihiro Ozaki, Christian W Huck
In the present work the performances of one benchtop and two different types of miniaturized near-infrared (NIR)-spectrometers were tested and compared for the first time by the determination of the rosmarinic acid (RA) content of dried and powdered Rosmarini folium. The recorded NIR spectra were utilized in hyphenation with multivariate data analysis (MVA) to calculate Partial Least Squares (PLS) regression models. Quality parameters obtained from Cross Validation (CV) revealed that the benchtop NIR-device "NIRFlex N-500 FT-NIR spectrometer" achieved the best result with a R(2) of 0...
December 15, 2016: Analyst
Arash Hanifi, Uday Palukuru, Cushla McGoverin, Michael Shockley, Eliot Frank, Alan Grodzinsky, Richard G Spencer, Nancy Pleshko
Articular cartilage degeneration causes pain and reduces the mobility of millions of people annually. Regeneration of cartilage is challenging, due in part to its avascular nature, and thus tissue engineering approaches for cartilage repair have been studied extensively. Current techniques to assess the composition and integrity of engineered tissues, including histology, biochemical evaluation, and mechanical testing, are destructive, which limits real-time monitoring of engineered cartilage tissue development in vitro and in vivo...
December 15, 2016: Analyst
Andrew Schmudlach, Jeremy Felton, Robert T Kennedy, Norman J Dovichi
Glucotoxicity is a causative agent of type-2 diabetes, where high glucose levels damage the islets of Langerhans resulting in oxidative damage and endoplasmic reticulum stress. We evaluated the secretomes of healthy CD-1 murine islets. Three experimental conditions were investigated in biological triplicate: a control incubated with 11 mM glucose, 1-day incubation with 25 mM glucose, and 2-day incubation with 25 mM glucose. An SDS-based, filter-aided sample preparation protocol was used to prepare secretomes for analysis...
December 14, 2016: Analyst
Marco Realini, Claudia Conti, Alessandra Botteon, Chiara Colombo, Pavel Matousek
We present, for the first time, a portable full micro-Spatially Offset Raman Spectroscopy (micro-SORS) prototype permitting the in situ analysis of thin, highly turbid stratified layers at depths not accessible to conventional Raman microscopy. The technique is suitable for the characterisation of painted layers in panels, canvases and mural paintings, painted statues and decorated objects in cultural heritage or stratified polymers, and biological, catalytic and forensics samples where invasive analysis is undesirable or impossible to perform...
December 14, 2016: Analyst
Ying Zhou, Kondapa Naidu Bobba, Xue Wei Lv, Dan Yang, Nithya Velusamy, Jun Feng Zhang, Sankarprasad Bhuniya
We developed a nitroreductase responsive theranostic probe 1; it comprises biotinylated rhodol in conjunction with p-nitrobenzyl functionality. The probe 1 showed a remarkable fluorescence 'turn-on' signal in the presence of nitroreductase under physiological conditions. The probe is considerably stable within a wide biological pH range (6-8) and also is very sensitive toward a reducing micro-environment e.g. liver microsome. Further, it enables providing cellular and in vivo nematode images in a reducing microenvironment...
December 14, 2016: Analyst
K Muneeruddin, C E Bobst, R Frenkel, D Houde, I Turyan, Z Sosic, I A Kaltashov
Detailed profiling of both enzymatic (e.g., glycosylation) and non-enzymatic (e.g., oxidation and deamidation) post-translational modifications (PTMs) is frequently required for the quality assessment of protein-based drugs. Challenging as it is, this task is further complicated for the so-called second-generation biopharmaceuticals, which also contain "designer PTMs" introduced to either enhance their pharmacokinetic profiles (e.g., PEGylated proteins) or endow them with therapeutic activity (e.g., protein-drug conjugates)...
December 14, 2016: Analyst
Austen L Davis, Wenjie Liu, William F Siems, Brian H Clowers
Using a linearly swept chirp function to modulate a Bradbury-Nielsen (BN) ion gate and application of a common signal processing technique (cross-correlation), we outline a method for obtaining high resolution IMS-MS spectra with ion gate duty cycles approaching 50%. Correlation IMS (CIMS) offers advantages over current multiplexing approaches in IMS-MS, which include the Hadamard and Fourier transforms, by minimizing transform artifacts while maintaining high ion throughput. Although cross-correlation techniques have been utilized previously in the field of IMS, to the best of our knowledge, this approach has not been utilized to obtain spectrum that resembles traditional IMS spectrum with resolving powers approaching the theoretical limit...
December 14, 2016: Analyst
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