We have located links that may give you full text access.
[Study on the survival rate of random flap using pre-injection of ADSCs].
Zhonghua Yi Xue za Zhi [Chinese medical journal] 2016 September 28
Objective: To determine the effects of adipose-derived stem cells (ADSCs) on the survival rate of flaps by transplanting ADSCs into the experimental flaps pre-operatively. Methods: ADSCs were isolated from fresh human fat and cultured in vitro . Their morphological characters were observed, and flowcytometry and differentiation test and evaluation were conducted. A total of 40 BALB/c mice were divided into 5 groups randomly with each of 8 mice. 1 cm×4 cm random skin flap was designed on the mice back, ADSCs were injected in advance into subcutaneous tissues of 3 groups, the operation was performed on the 2nd, 5th and 7th day after the ADSCs injection, meanwhile skin flap operation was conducted in the immediate injection group and control group. Survival condition of the skin flaps was observed and von willebrand factor (vWF) staining was used to detect the number of micro-vessels in the skin flap. The enzyme-linked immuno sorbent assay (ELISA) method was used to detect the concentration of vascular endothelial growth factor (VEGF) in the skin flap. Results: The immediate injection group had a significantly higher survival rate of skin flap than the pre-injection groups and the control group [(51.5±6.3)% vs (38.3±6.7)%, (21.0±2.9)%, (30.0±3.9)% and (34.5±4.2)%, all P <0.01]. The immediate injection group had a significantly higher number of micro-vessels in the skin flap than the pre-injection groups and the control group [(21.0±4.3) vs (17.5±3.9), (9.9±2.6), (13.9±2.6) and (16.1±3.3)/LPF, all P <0.01]. The laboratory results of the concentration of VEGF in skin flap kept a consistent tendency with the flap survival rate. Conclusion: Pre-injection with the ADSCs into the skin flap prevents the survival of the flap to a certain extend and does not show the function of promoting tissue angiogenesis.
Full text links
Related Resources
Trending Papers
Consensus Statement on Vitamin D Status Assessment and Supplementation: Whys, Whens, and Hows.Endocrine Reviews 2024 April 28
The Tricuspid Valve: A Review of Pathology, Imaging, and Current Treatment Options: A Scientific Statement From the American Heart Association.Circulation 2024 April 26
Intravenous infusion of dexmedetomidine during the surgery to prevent postoperative delirium and postoperative cognitive dysfunction undergoing non-cardiac surgery: a meta-analysis of randomized controlled trials.European Journal of Medical Research 2024 April 19
Interstitial Lung Disease: A Review.JAMA 2024 April 23
Management of Diverticulitis: A Review.JAMA Surgery 2024 April 18
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app