We have located links that may give you full text access.
Journal Article
Research Support, U.S. Gov't, P.H.S.
Characterization of extracellular glucosyltransferase activity of Steptococcus mutans.
Infection and Immunity 1975 October
The extracellular glycosyltransferase activity of Sterptococcus mutans GS-5 has been resolved into two non-overlapping fractions after gel filtration chromatography on Bio Gel A-15 columns. The activity eluting in the void volume, fraction A, was highly aggregated and synthesized both soluble and insoluble glucans. The activity retarded by the resin, fraction B, synthesized only soluble glucan. Almost all of the extracellular glucosyltransferase activity was eluted in the void volume when the cells were grown in Todd-Hewitt medium. However, most of the activity migrated as the lower-molecular-weight species when cells were grown under conditions which inhibit insoluble glucan formation. The activities in both fractions had identical temperature and pH optima as well as similar Km values for sucrose. Fraction A synthesized both alpha-1,3- and alpha-1,6- linked glucans, whereas fraction B catalyzed alpha-1,6-glucan formation. Fraction B has been purified to near homogeneity and is also aggregated with a subunit molecular weight of 45,000. The properties of the glucosyltransferases in both fractions are discussed in terms of the role of the enzymes in both soluble and insoluble glucan formation.
Full text links
Related Resources
Trending Papers
Interstitial Lung Disease: A Review.JAMA 2024 April 23
Review article: Recent advances in ascites and acute kidney injury management in cirrhosis.Alimentary Pharmacology & Therapeutics 2024 March 26
Executive Summary: State-of-the-Art Review: Unintended Consequences: Risk of Opportunistic Infections Associated with Long-term Glucocorticoid Therapies in Adults.Clinical Infectious Diseases 2024 April 11
Clinical practice guidelines on the management of status epilepticus in adults: A systematic review.Epilepsia 2024 April 13
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app