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Single Cell Analysis of Proteoforms.
Journal of Proteome Research 2024 March 19
We introduce single cell Proteoform imaging Mass Spectrometry ( scPiMS ), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells dropcast onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enable a scalable approach to single cell proteomics. This new scPiMS platform addresses the throughput bottleneck in single cell proteomics and boosts the cell processing rate by several fold while accessing protein composition with higher coverage.
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