Green cleanup of styrene-contaminated soil by carbon-based nanoscale zero-valent iron and phytoremediation: Sunn hemp ( Crotalaria juncea) , zinnia ( Zinnia violacea Cav.), and marigold ( Tagetes erecta L. ) .

Accidental chemical spills can result in styrene-contaminated soil. Styrene negatively affects human health and the environment. The objective of this study was to remediate styrene-contaminated soil using a combination of activated carbon-based nanoscale zero-valent iron (nZVI-AC) and phytoremediation by sunn hemp ( Crotalaria juncea ), zinnia ( Zinnia violacea Cav.) and marigolds ( Tagetes erecta L.). The results showed that all three plant types could potentially increase the removal efficiency of styrene-contaminated soil. At 28 days, all three plants showed complete removal of styrene from the soil with 1 g/kg of nZVI-AC, activated carbon-based nZVI synthesized by tea leaves ( Camellia sinensis ) (T-nZVI-AC), or activated carbon-based nZVI synthesized by red Thai holy basil ( Ocimum tenuiflorum L.) (B-nZVI-AC). However, styrene removal efficiencies of sunn hemp, zinnia, and marigold without carbon-based nZVI were 30%, 67%, and 56%, respectively. Statistical analysis (ANOVA) revealed that the removal efficiencies differed significantly from those of phytoremediation alone. With the same removal efficiency (100%), the biomass of sunn hemp in nano-phytoremediation treatments differed by approximately 55%, whereas the biomass of zinnia differed by >67%, compared with that of the control experiment. For marigold, the difference in biomass was only 30%. Styrene was adsorbed on surface of soil and AC and then further oxidized under air-water-nZVI environment, while phytovolatilization played an important role in transporting the remaining styrene from the contaminated soil to the air. Marigold was used as an alternative plant for the nano-phytoremediation of styrene-contaminated soil because of its sturdy nature, high biomass, tolerance to toxic effects, and ease of cultivation. Remediation of one cubic meter of styrene-contaminated soil by a combination of carbon-based nanoscale zero-valent iron and phytoremediation by marigolds emitted 0.0027 kgCO2 /m3 .