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Marie-Michèle Tanguay Bernard, Mireille Luc, Jean-Daniel Carrier, Louise Fournier, Arnaud Duhoux, Elodie Côté, Olivier Lessard, Catherine Gibeault, Christian Bocti, Pasquale Roberge
Background: Benzodiazepines are among the most commonly prescribed drugs for anxiety disorders. While they are indicated as adjunctive treatment for short-term use according to clinical practice guidelines, previous studies have shown patterns of long-term use of benzodiazepines, which is problematic due to side effects, dependence and potential of abuse. The aims of this study were to examine among a large sample of primary care adults suffering from anxiety disorders: 1) benzodiazepine use patterns; and 2) correlates of long-term benzodiazepine use...
July 2018: Heliyon
Xi Li, Dongqin Tang, Yimin Shi
Quantitative real-time PCR (qRT-PCR) has been a widely used accurate technique for gene expression analysis in various species. However, its results require data normalization by reliable reference genes. Despite the horticultural importance of Narcissus pseudonarcissu , and genome sequence has become available for the species, no gene expression study based on the stability of reference genes in qRT-PCR has been conducted. To boost the use of qRT-PCR in N. pseudonarcissu , we uncovered eight commonly used candidate reference genes for their stability...
July 2018: Heliyon
Subiyanto, Hermanto, Ulfah Mediaty Arief, Ahmad Yazidun Nafi
In the last few years, the decrease in the land use of soybean affected the reduced of soybean production. Land suitability assessment is an effort to increase the soybean production since the manual method was less accurate. This study aims to apply fuzzy sets and AHP to improve the accuracy of the assessment process of land suitability for soybean crops. The value of five sub-criteria converted into fuzzy sets for standardization process. The weighting by AHP performed to determine the importance level of sub-criteria...
July 2018: Heliyon
Gabrielle Gauvin-Rossignol, Philippe Legros, Jean Ruel, Marc-André Fortin, André Bégin-Drolet
Alginate-based hydrogels are widely used for the development of biomedical scaffolds in regenerative medicine. The use of sugar glass as a sacrificial template for fluidic channels fabrication within alginate scaffolds remains a challenge because of the premature dissolution of sugar by the water contained in the alginate as well as the relatively slow internal gelation rate of the alginate. Here, a new and simple method, based on a sugar glass fugitive ink loaded with calcium chloride to build sacrificial molds, is presented...
July 2018: Heliyon
Arisa Sakamoto, Rui Yamaguchi, Reona Yamaguchi, Shinji Narahara, Hiroyuki Sugiuchi, Yasuo Yamaguchi
RANTES is a key chemokine for atherosclerosis, and obesity is associated with progression of atherosclerosis. Substance P (SP) increases glucose uptake and accumulation of lipids in adipocytes, and SP may upregulate RANTES expression. This study investigated the mechanism of RANTES expression by human M1 macrophages stimulated with SP. SP upregulated RANTES protein expression, whereas aprepitant (an NK1R antagonist) blunted this response. Pretreatment of macrophages with BIRB796 (a combined p38γ/p38δ inhibitor) led to a significant decrease of RANTES expression...
July 2018: Heliyon
Dilli Ram Thapa, Xiaxin Tao, Feng Fan, Zhengru Tao
On 25 April 2015, a large earthquake with moment - magnitude (Mw ) 7.8 occurred at Gorkha (latitude 28.23° N and longitude 84.73° E) in Central Nepal. Just 17 days later, another large earthquake of Mw 7.3 occurred at Dolakha (latitude 27.80° N and longitude 86.06° E), about 140 km away from the previous epicenter. In this study, we deal with the aftershocks of these two earthquakes that occurred in the region (27°-28.5°N, 84°-87°E) spanning the period from 25 April 2015 to 28 May 2016 to find the spatiotemporal distribution patterns, the size distribution ( b -value), and the aftershock decay rate ( p -value) of this sequence...
July 2018: Heliyon
Mohun Ramratnam, Barrett Kenny, John W Kyle, Brandi Wiedmeyer, Timothy A Hacker, David Y Barefield, Elizabeth M McNally, Jonathan C Makielski
ATP-sensitive potassium channels found in both the sarcolemma (sarcKATP ) and mitochondria (mitoKATP ) of cardiomyocytes are important mediators of cardioprotection during ischemic heart disease. Sulfonylurea receptor isoforms (SUR2), encoded by Abcc9 , an ATP-binding cassette family member, form regulatory subunits of the sarcKATP channel and are also thought to regulate mitoKATP channel activity. A short-form splice variant of SUR2 (SUR2A-55) was previously shown to target mitochondria and display diaxoxide and ATP insensitive KATP activity when co-expressed with the inward rectifier channels Kir6...
July 2018: Heliyon
Sonia Podvin, Aneta Wojnicz, Vivian Hook
Proteases are required to generate active peptide neurotransmitters, known as neuropeptides, from pro-neuropeptides. Model animal systems have recently illustrated roles for the cathepsin V (CTSV) and cathepsin L (CTSL) cysteine proteases, combined with the serine proteases PC1/3 (PCSK1) and PC2 (PCSK2), and exopeptidases in the production of neuropeptides. There is notable interest in the human-specific cathepsin V gene which is not present in rodent and other animal models used in prior studies of neuropeptide production...
July 2018: Heliyon
Wolfram Siede
Handling of the medically important dermatophyte Trichophyton rubrum in the laboratory typically requires the generation of spores - for storage, treatment and plating when needed. The described method allows technically simple but efficient generation and harvesting of microconidia by cutting holes in Sabouraud dextrose agar medium that is covered by a mature T. rubrum mycelium.
July 2018: Heliyon
Elias E Elemike, Henry U Nwankwo, Damian C Onwudiwe
Three Schiff base compounds, N 1 ,N 2 -bis(3-nitrobenzylidene)phenylene diamine (NBBA), 2-methyl- N -(3-nitrobenzylidene)aniline (MNBA) and N -(2-chlorobenzylidene)-4-nitroaniline (CBNA) were synthesized, characterised and applied for the first time as potential mild steel (MS) corrosion inhibitors in 1 M HCl at 30 °C. Fourier transform infra-red (FTIR), 1 H, 13 C Nuclear magnetic resonance (NMR) and Mass spectrometry (MS) were used for the characterisation of the compounds. The electrochemical studies and evaluation of corrosion inhibition potency were achieved using potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) techniques...
July 2018: Heliyon
Jenifer Trepiana, M Begoña Ruiz-Larrea, José Ignacio Ruiz-Sanz
Currently natural products derived from plants are receiving huge attention because of their antitumor activities. In previous work we reported that an aqueous leaf extract of Vismia baccifera induced toxicity in HepG2. The present study focuses on the mechanisms of the cytotoxic actions induced by the extract. Results showed that V. baccifera was innocuous in non-transformed human HH4 hepatocytes. In HepG2 it caused deregulation of antioxidant status (increasing superoxide dismutase expression and decreasing glutathione levels and glutathione peroxidase activity) and accumulation of reactive oxygen species, particularly hydrogen peroxide...
June 2018: Heliyon
Angela Collins, Edward K Wakeland, Prithvi Raj, Min S Kim, Jiwoong Kim, Naureen G Tareen, Lawson A B Copley
Background: Children with acute hematogenous osteomyelitis (AHO) have a broad spectrum of illness ranging from mild to severe. The purpose of this study is to evaluate the impact of genomic variation of Staphylococcus aureus on clinical phenotype of affected children and determine which virulence genes correlate with severity of illness. Methods: De novo whole genome sequencing was conducted for a strain of Community Acquired Methicillin Resistant Staphylococcus aureus (CA-MRSA), using PacBio Hierarchical Genome Assembly Process (HGAP) from 6 Single Molecule Real Time (SMRT) Cells, as a reference for DNA library assembly of 71 Staphylococcus aureus isolates from children with AHO...
June 2018: Heliyon
Sarah A Johnson, Mark R Ellersieck, Cheryl S Rosenfeld
Bisphenol A (BPA) is a pervasive industrial chemical used in many common household items. To examine how early exposure to BPA and ethinyl estradiol (EE, estrogen in birth control pill) might affect biparental care, effects of these chemicals in male and female California mice ( Peromyscus californicus ), who are monogamous and biparental, were examined. California mice exposed during pre- and peri-natal life to BPA at an environmentally relevant concentration or EE show later disrupted biparental behaviors...
June 2018: Heliyon
Ramon Guirado, Héctor Carceller, Esther Castillo-Gómez, Eero Castrén, Juan Nacher
The quantification of the expression of different molecules is a key question in both basic and applied sciences. While protein quantification through molecular techniques leads to the loss of spatial information and resolution, immunohistochemistry is usually associated with time-consuming image analysis and human bias. In addition, the scarce automatic software analysis is often proprietary and expensive and relies on a fixed threshold binarization. Here we describe and share a set of macros ready for automated fluorescence analysis of large batches of fixed tissue samples using FIJI/ImageJ...
June 2018: Heliyon
Yaoyao Zhan, Yingying Li, Dongyao Cui, Qiantong Pei, Jingxian Sun, Weijie Zhang, Yaqing Chang
MicroRNAs (miRNAs) play critical roles in regulating many bio-processes of eukaryotes. The sea urchin Strongylocentrotus intermedius (an important fishery resource) is of great economic importance in Japan, North Korea, Russia, and China. In the current study, miRNAs of tube foot in S. intermedius were firstly identified and characterized. Data in this study can provide more genomic information for the further understanding of the complex regulation network in sea urchins and present a new way for monitoring the health status of cultured sea urchins...
June 2018: Heliyon
Gennadi Glinsky, Jens Durruthy-Durruthy, Mark Wossidlo, Edward J Grow, Jason L Weirather, Kin Fai Au, Joanna Wysocka, Vittorio Sebastiano
Chromosome instability and aneuploidies occur very frequently in human embryos, impairing proper embryogenesis and leading to cell cycle arrest, loss of cell viability, and developmental failures in 50-80% of cleavage-stage embryos. This high frequency of cellular extinction events represents a significant experimental obstacle challenging analyses of individual cells isolated from human preimplantation embryos. We carried out single cell expression profiling of 241 individual cells recovered from 32 human embryos during the early and late stages of viable human blastocyst (VHB) differentiation...
June 2018: Heliyon
Kazuko Shichijo, Toshihiro Takatsuji, Manabu Fukumoto, Masahiro Nakashima, Mutsumi M Matsuyama, Ichiro Sekine
Background: Radiation doses received by Hiroshima and Nagasaki atomic bomb survivors has been evaluated from data related only to external exposure because there was no reliable evidence for internal exposure in atomic bomb victims. However, we assumed that the contribution of internal exposure cannot be ruled out. Methods: Autoradiography was carried out with the 70-year old paraffin-embedded specimens taken from Nagasaki atomic bomb victims who died within 5 months after the bombing...
June 2018: Heliyon
Michael Osei Adu, Paul Agu Asare, Elvis Asare-Bediako, Godwin Amenorpe, Frank Kwekucher Ackah, Emmanuel Afutu, Mishael Nyarko Amoah, David Oscar Yawson
The development of cassava genotypes with root system traits that increase soil resource acquisition could increase yields on infertile soils but there are relatively few work that has quantified cassava root system architecture (RSA). We used an easily adaptable and inexpensive protocol to: (i) measure genotypic variation for RSA and shoot traits of a range of cassava genotypes; and (ii) identify candidate variables that contribute the largest share of variance. Cassava genotypes were grown in soil-filled pots, maintained at 70% field capacity...
June 2018: Heliyon
Brajogopal Samanta, Punyasloke Bhadury
Sundarbans, the world's largest mangrove deltaic region, is one of the most productive ecosystems in tropical and subtropical latitudes and also serve as a nursery ground for rich coastal fisheries. In this study, we highlighted diatom assemblages from the Indian part of Sundarbans Biosphere Reserve (SBR) area for the first time based on light microscopy and rbc L gene sequencing and phylogeny. In total, 15 diatom species (11 centric forms and 4 pennate forms) were documented using light microscopy, and 3 major clades of diatoms were detected in rbc L phylogeny...
June 2018: Heliyon
Dinesh Kumar, Pooja Sharma, Kunal Nepali, Girish Mahajan, Mubashir J Mintoo, Amarinder Singh, Gurpreet Singh, Dilip M Mondhe, Gurdarshan Singh, Subheet K Jain, Girish K Gupta, Fidele Ntie-Kang
In an effort to discover an effective and selective antitumour agent, synthesis and anti-cancer potential of 4-(pyridin-4-yl)-6-(thiophen-2-yl) pyrimidin-2(1 H )-one ( SK-25 ), which has been reported earlier by us with significant cytotoxicity towards MiaPaCa-2 malignant cells, with an IC50 value of 1.95 μM and was found to instigate apoptosis. In the present study, the antitumour efficacy of SK-25 was investigated on Ehrlich ascites tumour (EAT, solid), Sarcoma 180 (solid) tumour and Ehrlich ascites carcinoma...
June 2018: Heliyon
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