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Ex vivo sensing and imaging of corneal scar tissues using terahertz time domain spectroscopy.
Pulsed terahertz (THz) broadband spectroscopy and imaging were applied to investigate ex vivo rabbit corneal scar tissues. Scars with different depths and densities were created by laser ablation on four corneal samples while two corneal samples untouched were used as control. The THz time-domain spectroscopy and reconstructed images illustrated the scar tissue density and gave out depth variation distribution profiles. Calculated absorption coefficient spectra displayed extra absorption peaks compared with control corneas and refractive index spectra showed the optical properties changed at the corneal scar locations. THz spectroscopy and imaging demonstrated adequate contrast in reviewing the scar densities and locations and showed potential applications in depicting spatial distribution and composition changes of corneal scars.
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