Troy L Carter, Giuseppe Verdile, David Groth, Alexey Bogush, Stefani Thomas, Patrick Shen, Paul E Fraser, Paul Mathews, Ralph A Nixon, Michelle E Ehrlich, John B J Kwok, Peter St George-Hyslop, Peter Schofield, Yueming Li, Austin Yang, Ralph N Martins, Sam Gandy
Presenilins are polytopic, integral proteins that control intramembranous proteolysis at the "gamma-" and "epsilon-" cleavage sites of the Alzheimer amyloid-beta precursor protein (APP) to yield amyloid-beta peptide (Abeta) and the APP intracellular domain (AICD). We have overexpressed a constitutively active, pathogenic form of PS1 (known as PS1 Delta exon 9) together with its substrate, APP-C99, in Spodoptera frugiperda (Sf9) cells. Sf9 cells have been reported to lack endogenous gamma-secretase, an unexpected finding since there exists an insect homologue of PS1...
October 2004: Alzheimer Disease and Associated Disorders