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https://www.readbyqxmd.com/read/28088640/bioethanol-production-from-cellulosic-hydrolysates-by-engineered-industrial-saccharomyces-cerevisiae
#1
Ye-Gi Lee, Yong-Su Jin, Young-Lok Cha, Jin-Ho Seo
Even though industrial yeast strains exhibit numerous advantageous traits for the production of bioethanol, their genetic manipulation has been limited. This study demonstrates that an industrial polyploidy Saccharomyces cerevisiae JHS200 can be engineered through Cas9 (CRISPR associated protein 9)-based genome editing. Specifically, we generated auxotrophic mutants and introduced a xylose metabolic pathway into the auxotrophic mutants. As expected, the engineered strain (JX123) enhanced ethanol production from cellulosic hydrolysates as compared to other engineered haploid strains...
December 18, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28070851/development-of-a-sustained-antiplaque-and-antimicrobial-chewing-gum-of-a-decapeptide
#2
Abeer M Al-Ghananeem, Kai P Leung, Jabar Faraj, Patrick P DeLuca
The objective of this paper was to design a chewing gum formulation delivery system in situations where typical dental hygiene practice is not practical. Thus, an analog of decapeptide KSL (KSL-W), known to possess antimicrobial and antiplaque activity, was incorporated into a chewing gum formulation containing cetylpyridinium chloride (CPC). The effect of the excipients, xylitol, and peppermint oil on active ingredients in vitro release was also assessed. Gum formulations were prepared with different excipient parameters, including heating xylitol and gum base at 65 or 85°C, using ground and unground xylitol, and the addition of 1...
January 9, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28070721/enhanced-xylose-fermentation-by-engineered-yeast-expressing-nadh-oxidase-through-high-cell-density-inoculums
#3
Guo-Chang Zhang, Timothy L Turner, Yong-Su Jin
Accumulation of reduced byproducts such as glycerol and xylitol during xylose fermentation by engineered Saccharomyces cerevisiae hampers the economic production of biofuels and chemicals from cellulosic hydrolysates. In particular, engineered S. cerevisiae expressing NADPH-linked xylose reductase (XR) and NAD(+)-linked xylitol dehydrogenase (XDH) produces substantial amounts of the reduced byproducts under anaerobic conditions due to the cofactor difference of XR and XDH. While the additional expression of a water-forming NADH oxidase (NoxE) from Lactococcus lactis in engineered S...
January 9, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28054259/enhanced-production-of-xylitol-from-poplar-wood-hydrolysates-through-a-sustainable-process-using-immobilized-new-strain-candida-tropicalis-ufmg-bx-12-a
#4
Sai Swaroop Dalli, Silvio Silverio da Silva, Bijaya K Uprety, Sudip K Rakshit
A new strain, Candida tropicalis UFMG BX 12-a, was found to produce higher yields of xylitol on poplar wood hemicellulose hydrolysate. The hemicellulose hydrolysate liquor was detoxified using a novel method we developed, involving vacuum evaporation and solvent separation of inhibitors which made the hydrolysate free of toxins while retaining high concentrations of fermentable sugars. The effect of the detoxification method on the fermentation was also reported and compared to well-known methods reported in literature...
January 4, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28053663/utilization-of-xylose-by-engineered-strains-of-ashbya-gossypii-for-the-production-of-microbial-oils
#5
David Díaz-Fernández, Patricia Lozano-Martínez, Rubén M Buey, José Luis Revuelta, Alberto Jiménez
BACKGROUND: Ashbya gossypii is a filamentous fungus that is currently exploited for the industrial production of riboflavin. The utilization of A. gossypii as a microbial biocatalyst is further supported by its ability to grow in low-cost feedstocks, inexpensive downstream processing and the availability of an ease to use molecular toolbox for genetic and genomic modifications. Consequently, A. gossypii has been also introduced as an ideal biotechnological chassis for the production of inosine, folic acid, and microbial oils...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28031033/the-aceii-recombinant-trichoderma-reesei-qm9414-strains-with-enhanced-xylanase-production-and-its-applications-in-production-of-xylitol-from-tree-barks
#6
Lili Xiong, Ayyappa Kumar Sista Kameshwar, Xi Chen, Zhiyun Guo, Canquan Mao, Sanfeng Chen, Wensheng Qin
BACKGROUND: ACEII transcription factor plays a significant role in regulating the expression of cellulase and hemicellulase encoding genes. Apart from ACEII, transcription factors such as XYR1, CRE1, HAP2/3/5 complex and ACEI function in a coordinated pattern for regulating the gene expression of cellulases and hemicellulases. Studies have demonstrated that ACEII gene deletion results in decreased total cellulase and xylanase activities with reduced transcript levels of lignocellulolytic enzymes...
December 28, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/28004152/comparative-transcriptomes-reveal-novel-evolutionary-strategies-adopted-by-saccharomyces-cerevisiae-with-improved-xylose-utilization-capability
#7
Wei-Yi Zeng, Yue-Qin Tang, Min Gou, Zhao-Yong Sun, Zi-Yuan Xia, Kenji Kida
Production of ethanol from xylose by recombinant Saccharomyces cerevisiae is suboptimal with slow fermentation rate, compared with that from glucose. In this study, a strain-expressing Scheffersomyces stipitis xylose reductase-xylitol dehydrogenase (XR-XDH) pathway was subjected to adaptive evolution on xylose; this approach generated populations with the significantly improved cell growth and ethanol production rate. Mutants were isolated, and the best one was used for sporulation to generate eight stable mutant strains with improved xylose fermentation ability...
December 21, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27987653/free-radical-scavenging-injectable-hydrogels-for-regenerative-therapy
#8
Remya Komeri, Finosh Gnanaprakasam Thankam, Jayabalan Muthu
Pathological free radicals generated from inflamed and infarcted cardiac tissues interferes natural tissue repair mechanisms. Hypoxic microenvironment at the injured zone of non-regenerating cardiac tissues hinders the therapeutic attempts including cell therapy. Here we report an injectable, cytocompatible, free radical scavenging synthetic hydrogel formulation for regenerative therapy. New hydrogel (PEAX-P) is prepared with D-xylitol-co-fumarate-co-poly ethylene adipate-co-PEG comaromer (PEAX) and PEGDiacrylate...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27979302/3-6-anhydro-l-galactose-a-rare-sugar-from-agar-a-new-anticariogenic-sugar-to-replace-xylitol
#9
Eun Ju Yun, Ah Reum Lee, Jung Hyun Kim, Kyung Mun Cho, Kyoung Heon Kim
The significance for anticariogenic sugar substitutes is growing due to increasing demands for dietary sugars and rising concerns of dental caries. Xylitol is widely used as an anticariogenic sugar substitute, but the inhibitory effects of xylitol on Streptococcus mutans, the main cause of tooth decay, are exhibited only at high concentrations. Here, the inhibitory effects of 3,6-anhydro-l-galactose (AHG), a rare sugar from red macroalgae, were evaluated on S. mutans, in comparison with those of xylitol. In the presence of 5g/l of AHG, the growth of S...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27977916/spore-displayed-enzyme-cascade-with-tunable-stoichiometry
#10
Long Chen, Ashok Mulchandani, Xin Ge
Taking the advantages of inert and stable nature of endospores, we developed a biocatalysis platform for multiple enzyme immobilization on Bacillus subtilis spore surface. Among B. subtilis outer coat proteins, CotG mediated a high expression level of Clostridium thermocellum cohesin (CtCoh) with a functional display capability of ∼10(4) molecules per spore of xylose reductase C. termocellum dockerin fusion protein (XR-CtDoc). By co-immobilization of phosphite dehydrogenase (PTDH) on spore surface via Ruminococcus flavefaciens cohesin-dockerin modules, regeneration of NADPH was achieved...
December 15, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27955868/engineered-kluyveromyces-marxianus-for-pyruvate-production-at-elevated-temperature-with-simultaneous-consumption-of-xylose-and-glucose
#11
Biao Zhang, Yelin Zhu, Jia Zhang, Dongmei Wang, Lianhong Sun, Jiong Hong
Xylose and glucose from lignocellulose are sustainable sources for production of pyruvate, which is the starting material for the synthesis of many drugs and agrochemicals. In this study, the pyruvate decarboxylase gene (KmPDC1) and glycerol-3-phosphate dehydrogenase gene (KmGPD1) of Kluyveromyces marxianus YZJ051 were disrupted to prevent ethanol and glycerol accumulation. The deficient growth of PDC disruption was rescued by overexpressing mutant KmMTH1-ΔT. Then pentose phosphate pathway and xylitol dehydrogenase SsXYL2-ARS genes were overexpressed to obtain strain YZB053 which produced pyruvate with xylose other than glucose...
January 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27940144/glucose-specific-signaling-effects-on-delay-discounting-in-intertemporal-choice
#12
X T Xiao-Tian Wang, Gang Huangfu
We propose that decisions related to resource management (e.g., intertemporal choice between a smaller-and-sooner reward and a larger-and-later reward) are sensitive to and regulated by fluctuating blood glucose levels. Circulating glucose affects intertemporal choice by means of signaling body energy condition instead of serving as a replenishing resource for effortful cognitive processing. We intend to dissociate calorie-supplying functions from glucose-unique anticipatory effects on behavioral resource management, measured by delay discounting in making intertemporal choices...
February 1, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/27933669/characterization-of-nitrated-sugar-alcohols-by-atmospheric-pressure-chemical-ionization-mass-spectrometry
#13
Alla Ostrinskaya, Jude A Kelley, Roderick R Kunz
RATIONALE: The nitrated sugar alcohols mannitol hexanitrate (MHN), sorbitol hexanitrate (SHN) and xylitol pentanitrate (XPN) are in the same class of compounds as the powerful military grade explosive pentaerythritol tetranitrate (PETN) and the homemade explosive erythritol tetranitrate (ETN) but, unlike for PETN and ETN, ways to detect MHN, SHN and XPN by mass spectrometry (MS) have not been fully investigated. METHODS: Atmospheric-pressure chemical-ionization mass spectrometry (APCI-MS) was used to detect ions characteristic of nitrated sugar alcohols...
December 9, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/27932866/effect-of-peritoneal-dialysis-fluid-containing-osmo-metabolic-agents-on-human-endothelial-cells
#14
Mario Bonomini, Sara Di Silvestre, Pamela Di Tomo, Natalia Di Pietro, Domitilla Mandatori, Lorenzo Di Liberato, Vittorio Sirolli, Francesco Chiarelli, Cesare Indiveri, Assunta Pandolfi, Arduino Arduini
BACKGROUND: The use of glucose as the only osmotic agent in peritoneal dialysis (PD) solutions (PDSs) is believed to exert local (peritoneal) and systemic detrimental actions, particularly in diabetic PD patients. To improve peritoneal biocompatibility, we have developed more biocompatible PDSs containing xylitol and carnitine along with significantly less amounts of glucose and have tested them in cultured Human Vein Endothelial Cells (HUVECs) obtained from the umbilical cords of healthy (C) and gestational diabetic (GD) mothers...
2016: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/27931418/policy-on-the-use-of-xylitol
#15
(no author information available yet)
No abstract text is available yet for this article.
October 2016: Pediatric Dentistry
https://www.readbyqxmd.com/read/27920611/products-based-on-olive-oil-betaine-and-xylitol-in-the-post-radiotherapy-xerostomia
#16
Margarita Martín, Alicia Marín, Mario López, Olga Liñán, Felipe Alvarenga, David Büchser, Laura Cerezo
AIM: The objective of this study was determining if the use of products based in olive oil, betaine and xylitol are efficacious to decrease the impact of the dry mouth in the quality of life of the patients with xerostomia due to radiotherapy treatment. BACKGROUND: Following therapeutic irradiation of the head and neck, patients with profound xerostomia have complaints associated with oral dryness, speech, and taste. There is no strong evidence that any topical therapy is effective for relieving the symptom of dry mouth...
January 2017: Reports of Practical Oncology and Radiotherapy
https://www.readbyqxmd.com/read/27899337/furfural-and-glucose-can-enhance-conversion-of-xylose-to-xylitol-by-candida-magnoliae-tistr-5663
#17
Siwaporn Wannawilai, Wen-Chien Lee, Yusuf Chisti, Sarote Sirisansaneeyakul
Xylitol production from xylose by the yeast Candida magnoliae TISTR 5663 was enhanced by supplementing the fermentation medium with furfural (300mg/L) and glucose (3g/L with an initial mass ratio of glucose to xylose of 1:10) together under oxygen limiting conditions. In the presence of furfural and glucose, the final concentration of xylitol was unaffected relative to control cultures but the xylitol yield on xylose increased by about 5%. Supplementation of the culture medium with glucose alone at an initial concentration of 3g/L, stimulated the volumetric and specific rates of xylose consumption and the rate of xylitol production from xylose...
January 10, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/27896339/intramolecular-dehydration-of-biomass-derived-sugar-alcohols-in-high-temperature-water
#18
Aritomo Yamaguchi, Natsumi Muramatsu, Naoki Mimura, Masayuki Shirai, Osamu Sato
The intramolecular dehydration of biomass-derived sugar alcohols d-sorbitol, d-mannitol, galactitol, xylitol, ribitol, l-arabitol, erythritol, l-threitol, and dl-threitol was investigated in high-temperature water at 523-573 K without the addition of any acid catalysts. d-Sorbitol and d-mannitol were dehydrated into isosorbide and isomannide, respectively, as dianhydrohexitol products. Galactitol was dehydrated into anhydrogalactitols; however, the anhydrogalactitols could not be dehydrated into dianhydrogalactitol products because of the orientation of the hydroxyl groups at the C-3 and C-6 positions...
November 29, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27890595/the-formation-and-physical-stability-of-two-phase-solid-dispersion-systems-of-indomethacin-in-supercooled-molten-mixtures-with-different-matrix-formers
#19
Kristian Semjonov, Karin Kogermann, Ivo Laidmäe, Osmo Antikainen, Clare J Strachan, Henrik Ehlers, Jouko Yliruusi, Jyrki Heinämäki
Amorphous solid dispersions (SDs) are a promising approach to improve the dissolution rate of and oral bioavailability of poorly water-soluble drugs. In some cases multi-phase, instead of single-phase, SD systems with amorphous drug are obtained. While it is widely assumed that one-phase amorphous systems are desirable, two-phase systems may still potentially exhibit enhanced stability and dissolution advantages over undispersed systems. The objective of the present study was to understand the solid-state properties of two-phase SDs with amorphous drug and their relation to physical stability...
January 15, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27863495/anaerobic-poly-3-d-hydroxybutyrate-production-from-xylose-in-recombinant-saccharomyces-cerevisiae-using-a-nadh-dependent-acetoacetyl-coa-reductase
#20
Alejandro Muñoz de Las Heras, Diogo J Portugal-Nunes, Nathasha Rizza, Anders G Sandström, Marie F Gorwa-Grauslund
BACKGROUND: Poly-3-D-hydroxybutyrate (PHB) that is a promising precursor for bioplastic with similar physical properties as polypropylene, is naturally produced by several bacterial species. The bacterial pathway is comprised of the three enzymes β-ketothiolase, acetoacetyl-CoA reductase (AAR) and PHB synthase, which all together convert acetyl-CoA into PHB. Heterologous expression of the pathway genes from Cupriavidus necator has enabled PHB production in the yeast Saccharomyces cerevisiae from glucose as well as from xylose, after introduction of the fungal xylose utilization pathway from Scheffersomyces stipitis including xylose reductase (XR) and xylitol dehydrogenase (XDH)...
November 18, 2016: Microbial Cell Factories
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