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https://www.readbyqxmd.com/read/28433914/accumulation-of-sugar-from-pulp-and-xylitol-from-xylose-by-pyruvate-decarboxylase-negative-white-rot-fungus-phlebia-sp-mg-60
#1
Taku Tsuyama, Megumi Yamaguchi, Ichiro Kamei
Phlebia sp. MG-60 is a white-rot fungus that produces ethanol with high efficiency from lignocellulosic biomass without additional enzymes. Through engineering of this powerful metabolic pathway for fermentation in Phlebia sp. MG-60, chemical compounds other than ethanol could be produced. Here, we demonstrate sugar accumulation from unbleached hardwood kraft pulp and conversion of xylose to xylitol by pyruvate decarboxylase (pdc)-negative Phlebia sp. MG-60. We isolated Phlebia sp. strain MG-60-P2 from protoplasts to unify the protoplast phenotypes of the regenerated strains...
April 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28425351/metabolic-changes-in-methomyl-poisoned-rats-treated-by-vitamin-e
#2
J Weng, B Cai, J Chen, B Chen, H Wu, C Wen, F Sun, L Hu, C Chen, X Wang, Z Wang
In this study, we developed a serum and urine metabolomic method based on gas chromatography-mass spectrometry (GC-MS) combination with biomedical results to evaluate the effect of vitamin E treatment on methomyl poisoning rats. The rats were divided into three groups: the control group, methomyl poisoning group, and vitamin E treatment group. Partial least squares discriminate analysis (PLS-DA) showed that methomyl poisoning induced metabolic perturbations. Compared to the control group, based on the urinary metabolomics data, the level of ribitol, l-proline, xylitol, hydrocinnamic acid, 11-cis-octadecenoic acid, octadecanoic acid, and hexadecanoic acid of methomyl poisoning group increased, while the level of 2,3,4-trihydroxybutyric acid, ethanimidic acid, pantothenic acid, and retinoic acid decreased...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28403843/knowledge-about-preventive-dentistry-versus-self-reported-competence-in-providing-preventive-oral-healthcare-a-study-among-nepalese-dentists
#3
Madhu Wagle, Ganesh Acharya, Purusotam Basnet, Tordis A Trovik
BACKGROUND: Dentists' and dental healthcare providers' professional knowledge and attitude towards the prevention of oral diseases may have an impact on the oral health of the general population. The aim of this study was to describe Nepalese dentists' competency in giving preventive education and treatment to their patients, and to assess their level of knowledge about preventive dental health. METHODS: This was a cross-sectional study of 195 dentists (71 males and 124 females)...
April 12, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/28390875/effect-of-non-nutritive-sugars-to-decrease-the-survivorship-of-spotted-wing-drosophila-drosophila-suzukii
#4
Man-Yeon Choi, Siew Bee Tang, Seung-Joon Ahn, Kaushalya G Amarasekare, Peter Shearer, Jana C Lee
In this study, we investigated the effects of non-nutritive sugars and sugar alcohols on the survivorship of spotted wing drosophila, Drosophila suzukii, and found erythritol and erythrose as potentially insecticidal to the fly. In a dose-dependent study, erythritol and erythrose significantly reduced fly longevity, with 100% mortality with 1, 0.5, 0.1 & 0.05M doses after feeding for 7days. When sucrose and erythritol solutions were provided separately to flies for 7days, there was no effect on survivorship regardless of erythritol concentrations...
April 5, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28390807/xylitol-nasal-irrigation-in-the-treatment-of-chronic-rhinosinusitis
#5
Lin Lin, Xinyue Tang, Jinjin Wei, Fei Dai, Guangbin Sun
OBJECTIVE: To evaluate the efficacy of xylitol nasal irrigation (XNI) treatment on chronic rhinosinusitis (CRS) and to investigate the effect of XNI on nasal nitric oxide (NO) and inducible nitric oxide synthase (iNOS) mRNA in maxillary sinus. MATERIALS AND METHODS: Patients with CRS were enrolled and symptoms were assessed by Visual Analog Scale (VAS) and Sino-Nasal Outcome Test 22 (SNOT-22). Nasal NO and iNOS mRNA in the right maxillary sinus were also examined...
April 4, 2017: American Journal of Otolaryngology
https://www.readbyqxmd.com/read/28390459/effectiveness-of-xylitol-in-reducing-dental-caries-in-children
#6
Abdullah A Marghalani, Emilie Guinto, Minhthu Phan, Vineet Dhar, Norman Tinanoff
PURPOSE: The purpose of this study was to evaluate the effectiveness of xylitol in reducing dental caries in children compared to no treatment, a placebo, or preventive strategies. METHODS: MEDLINE via PubMed, Web of Science, and Cochrane Central Register of Controlled Trials (CENTRAL) were searched from January 1, 1995 through Sept. 26, 2016 for randomized and controlled trials on children consuming xylitol for at least 12 months. The primary endpoint was caries reduction measured by mean decayed, missing, and filled primary and permanent surfaces/ teeth (dmfs/t, DMFS/T, respectively)...
March 15, 2017: Pediatric Dentistry
https://www.readbyqxmd.com/read/28386802/description-of-hyphopichia-buzzinii-f-a-sp-nov-and-hyphopichia-homilentoma-comb-nov-the-teleomorph-of-candida-homilentoma
#7
Lucas R Ribeiro, Ana R O Santos, Marizeth Groenewald, Maudy Th H Smith, Carla A Lara, Aristóteles Góes-Neto, Noémie Jacques, Cécile Grondin, Serge Casaregola, Marc-André Lachance, Carlos A Rosa
During studies of the yeast diversity associated with rotting wood in Brazil and fruits, plants and insects in French Guiana, three strains of a new species were isolated. Analysis of the sequences of the internal transcribed spacer (ITS)-5.8S and D1/D2 domains of the large subunit of the rRNA gene showed that this species belongs to the genus Hyphopichia and its closest relative is Candida homilentoma. These species differ by 44 nucleotide substitutions in D1/D2 sequences. A new species Hyphopichia buzzinii f...
April 6, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28385593/a-genome-scale-model-of-geobacillus-thermoglucosidasius-c56-ys93-reveals-its-biotechnological-potential-on-rice-straw-hydrolysate
#8
Ahmad Ahmad, Hassan B Hartman, S Krishnakumar, David A Fell, Mark G Poolman, Shireesh Srivastava
Rice straw is a major crop residue which is burnt in many countries, creating significant air pollution. Thus, alternative routes for disposal of rice straw are needed. Biotechnological treatment of rice straw hydrolysate has potential to convert this agriculture waste into valuable biofuel(s) and platform chemicals. Geobacillus thermoglucosidasius is a thermophile with properties specially suited for use as a biocatalyst in lignocellulosic bioprocesses, such as high optimal temperature and tolerance to high levels of ethanol...
April 4, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28385229/co-production-of-chitin-glucan-complex-and-xylitol-by-komagataella-pastoris-using-glucose-and-xylose-mixtures-as-carbon-source
#9
Diana Araújo, Filomena Freitas, Chantal Sevrin, Christian Grandfils, Maria A M Reis
Komagataella pastoris was cultivated in glucose/xylose mixtures for production of chitin-glucan complex (CGC), a cell-wall polysaccharide. The culture preferred glucose as substrate for growth, resulting in high biomass yields (0.46-0.54g/g). After glucose depletion, xylose was consumed but no cell growth was observed, indicating K. pastoris was unable to use it for growth. Interestingly, concomitant with xylose consumption, xylitol synthesis was noticed, reaching a maximum concentration of 7.64g/L, with a yield on xylose of 0...
June 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28364889/streptococcus-mutans-forms-xylitol-resistant-biofilm-on-excess-adhesive-flash-in-novel-ex-vivo-orthodontic-bracket-model
#10
Cindy S F Ho, Yue Ming, Kelvin W C Foong, Vinicius Rosa, Truong Thuyen, Chaminda J Seneviratne
INTRODUCTION: During orthodontic bonding procedures, excess adhesive is invariably left on the tooth surface at the interface between the bracket and the enamel junction; it is called excess adhesive flash (EAF). We comparatively evaluated the biofilm formation of Streptococcus mutans on EAF produced by 2 adhesives and examined the therapeutic efficacy of xylitol on S mutans formed on EAF. METHODS: First, we investigated the biofilm formation of S mutans on 3 orthodontic bracket types: stainless steel preadjusted edgewise, ceramic preadjusted edgewise, and stainless steel self-ligating...
April 2017: American Journal of Orthodontics and Dentofacial Orthopedics
https://www.readbyqxmd.com/read/28364633/improved-xylitol-production-by-expressing-a-novel-d-arabitol-dehydrogenase-from-isolated-gluconobacter-sp-jx-05-and-co-biotransformation-of-whole-cells
#11
Xianghui Qi, Huanhuan Zhang, Tinashe Archbold Magocha, Yingfeng An, Junhua Yun, Miaomiao Yang, Yanbo Xue, Shuhua Liang, Wenjing Sun, Zheng Cao
In the present study, a novel ardh gene encoding d-arabitol dehydrogenase (ArDH) was cloned and expressed in Escherichia coli from a new isolated strain of Gluconobacter sp. JX-05. Sequence analysis revealed that ArDH containing a NAD(P)-binding motif and a classical active site motif belongs to the short-chain dehydrogenase family. Subsequently, the optimal pH and temperature, specific activities and kinetic parameter of ArDH were determined. In the co-biotransformation by the whole cells of BL21-ardh and BL21-xdh, 26...
March 21, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28330274/evaluation-of-some-lignocellulosic-byproducts-of-food-industry-for-microbial-xylitol-production-by-candida-tropicalis
#12
Kubra Eryasar, Seda Karasu-Yalcin
Some lignocellulosic food byproducts such as potato peels, wheat bran, barley bran and chestnut shells were evaluated as potential sources of xylose for microbial xylitol production by yeasts. Potential yeast strains were selected after screening xylitol production of some indigenous yeasts in a defined fermentation medium. Candida tropicalis strains gave the highest results with 83.28 and 54.07 g/L xylitol production from 100 g/L xylose. Lignocellulosic materials were exposed to acid hydrolysis at different conditions...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330223/multi-response-optimization-for-enhanced-xylitol-production-by-debaryomyces-nepalensis-in-bioreactor
#13
J Sharon Mano Pappu, Sathyanarayana N Gummadi
In this study, the optimization of different process variables-pH (4-6), aeration rate (200-550 rpm) and agitation rate (0.6-1.8 vvm) were investigated using rotating simplex method and uniform design method to enhance xylitol production from xylose by D. nepalensis in a batch stirred tank bioreactor. Maximum xylitol productivity (0.576 g L(-1) h(-1)) was obtained at pH 4.0, agitation 300 rpm and aeration 1.5 vvm by rotating simplex method. Individual optimum values of pH, agitation and aeration are 4...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330197/xylitol-production-by-saccharomyces-cerevisiae-overexpressing-different-xylose-reductases-using-non-detoxified-hemicellulosic-hydrolysate-of-corncob
#14
Anushree Kogje, Anand Ghosalkar
Xylitol production was compared in fed batch fermentation by Saccharomyces cerevisiae strains overexpressing xylose reductase (XR) genes from Candida tropicalis, Pichia stipitis, Neurospora crassa, and an endogenous gene GRE3. The gene encoding a xylose specific transporter (SUT1) from P. stipitis was cloned to improve xylose transport and fed batch fermentation was used with glucose as a cosubstrate to regenerate NADPH. Xylitol yield was near theoretical for all the strains in fed batch fermentation. The highest volumetric (0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330145/enhanced-xylitol-production-using-immobilized-candida-tropicalis-with-non-detoxified-corn-cob-hemicellulosic-hydrolysate
#15
Tatyaso Yewale, Shruti Panchwagh, Srinivasan Rajagopalan, Pradip B Dhamole, Rishi Jain
This study reports an industrially applicable non-sterile xylitol fermentation process to produce xylitol from a low-cost feedstock like corn cob hydrolysate as pentose source without any detoxification. Different immobilization matrices/mediums (alginate, polyvinyl alcohol, agarose gel, polyacrylamide, gelatin, and κ-carrageenan) were studied to immobilize Candida tropicalis NCIM 3123 cells for xylitol production. Amongst this calcium alginate, immobilized cells produced maximum amount of xylitol with titer of 11...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28328014/n-guanidino-derivatives-of-1-5-dideoxy-1-5-imino-d-xylitol-are-potent-selective-and-stable-inhibitors-of-%C3%AE-glucocerebrosidase
#16
Alen Sevšek, Luka Šrot, Jakob Rihter, Maša Čelan, Linda Quarles van Ufford, Ed E Moret, Nathaniel I Martin, Roland J Pieters
A series of lipidated guanidino and urea derivatives of 1,5-dideoxy-1,5-imino-d-xylitol were prepared from d-xylose using a concise synthetic protocol. Inhibition assays with a panel of glycosidases revealed that the guanidino analogues display potent inhibition against human recombinant β-glucocerebrosidase with IC50 values in the low nanomolar range. Related urea analogues of 1,5-dideoxy-1,5-imino-d-xylitol were also synthesized and evaluated in the same fashion and found to be selective for β-galactosidase from bovine liver...
March 22, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28327266/milk-sweetened-with-xylitol-a-proof-of-principle-caries-prevention-randomized-clinical-trial
#17
Donald L Chi, Graciela Zegarra, Elsa C Vasquez Huerta, Jorge L Castillo, Peter Milgrom, Marilyn C Roberts, Ailin R Cabrera-Matta, Ana P Merino
PURPOSE: To evaluate the efficacy of xylitol-sweetened milk as a caries-preventive strategy. METHODS: In this nine-month prospective proof-of-principle trial, Peruvian schoolchildren were randomized to one of five different milk groups: (1) eight g of xylitol per 200 mL milk once per day; (2) four g of xylitol per 100 mL milk twice per day; (3) eight g of sorbitol per 200 mL milk once per day; (4) four g of sorbitol per 100 mL milk twice per day; or (5) eight g of sucrose per 200 mL milk once per day...
September 15, 2016: Journal of Dentistry for Children
https://www.readbyqxmd.com/read/28325622/the-effect-of-different-nasal-irrigation-solutions-following-septoplasty-and-concha-radiofrequency-a-prospective-randomized-study
#18
Hanifi Kurtaran, K Serife Ugur, Ceyda Sel Yilmaz, Mesut Kaya, Alper Yuksel, Nebil Ark, Mehmet Gunduz
INTRODUCTION: Nasal irrigation solutions are widely used following endonasal surgery. These irrigation solutions remove infective debris and crusts, reducing the probability of synechia formation, and accelerate mucosal healing. OBJECTIVE: The aim of the present study was to compare the effects of nasal irrigation solutions with different contents following septoplasty and concha radiofrequency. METHODS: The present study was a prospective, randomized, controlled simple blind study of 120 patients who underwent septoplasty and bilateral concha radiofrequency...
February 22, 2017: Brazilian Journal of Otorhinolaryngology
https://www.readbyqxmd.com/read/28322366/the-efficacy-of-xylitol-xylitol-probiotic-and-fluoride-dentifrices-in-plaque-reduction-and-gingival-inflammation-in-children-a-randomised-controlled-clinical-trial
#19
Eda Arat Maden, Ceyhan Altun, Cengizhan Açikel
PURPOSE: The present prospective, randomised, placebo-controlled, clinical trial was designed to evaluate the clinical effects of a commercially available dentifrice containing fluoride, xylitol or xylitol-probiotic on the decrease of plaque and gingival inflammation in children between 13 and 15 years of age. MATERIALS AND METHODS: Forty-eight adolescents were randomly grouped into three groups of n = 16 each: study group A received xylitol (Xyliwhite) toothpaste; study group B received xylitol-probiotic (Periobiotic) toothpaste; and the control group C received fluoride (Colgate Max Fresh) toothpaste...
March 20, 2017: Oral Health & Preventive Dentistry
https://www.readbyqxmd.com/read/28303470/the-caries-preventive-effect-of-1-year-use-of-low-dose-xylitol-chewing-gum-a-randomized-placebo-controlled-clinical-trial-in-high-caries-risk-adults
#20
Fabio Cocco, Giovanna Carta, Maria Grazia Cagetti, Laura Strohmenger, Peter Lingström, Guglielmo Campus
OBJECTIVES: The caries preventive effect of long-term use (1 year) of low-dosage (2.5 g/die) of xylitol chewing gum in a high-caries-risk adult population was evaluated. MATERIALS AND METHODS: In this randomized clinical trial, 179 high-caries-risk adults were assigned to two experimental groups, xylitol and polyols. Caries status, salivary mutans streptococci (MS), and plaque pH were re-evaluated after 2 years from baseline in 66 xylitol and 64 polyol subjects...
March 16, 2017: Clinical Oral Investigations
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