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Cartilage regeneration

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https://www.readbyqxmd.com/read/29350587/recent-advances-in-3d-bioprinting-for-the-regeneration-of-functional-cartilage
#1
Ji Xiongfa, Zhu Hao, Zhao Liming, Xiao Jun
The field of regeneration for functional cartilage has progressed tremendously. Conventional approaches for regenerating the damaged tissue based on integrated manufacturing are limited by their inability to produce precise and customized biomimetic tissues. On the other hand, 3D bioprinting is a promising technique with increased versatility because it can co-deliver cells and biomaterials with proper compositions and spatial distributions. In the present article, we review recent progress in the complete 3D printing process involved in functional cartilage regeneration, including printing techniques, biomaterials and cells...
January 19, 2018: Regenerative Medicine
https://www.readbyqxmd.com/read/29343182/fibronectin-enhances-cartilage-repair-by-activating-progenitor-cells-through-integrin-%C3%A9-5%C3%AE-1-receptor
#2
Tianqi Tao, Yang Li, Chang Gui, Yong Ma, Yingbin Ge, Hanhao Dai, Kaibin Zhang, Jing Du, Yang Guo, Yiqiu Jiang, Jianchao Gui
This study aimed to determine the effect of fibronectin (FN) on cartilage regeneration through the activation of chondrogenic progenitor cells (CPCs). Cells were isolated from the knee cartilage of mice and cultured in the presence of various concentrations of FN. Proliferation, migration and chondrogenic differentiation assays were performed in vitro. In some experiments, CPCs were pre-incubated with anti-integrin ɑ5β1 antibody for 60 min prior to FN treatment to block the integrin ɑ5β1 receptor. Soluble FN was mixed with Pluronic F-127 and injected into the joint cavity in an early-stage osteoarthritis (OA) model...
January 18, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29342064/minimally-manipulated-bone-marrow-concentrate-compared-with-microfracture-treatment-of-full-thickness-chondral-defects-a-one-year-study-in-an-equine-model
#3
Constance R Chu, Lisa A Fortier, Ashley Williams, Karin A Payne, Taralyn M McCarrel, Megan E Bowers, Diego Jaramillo
BACKGROUND: Microfracture is commonly performed for cartilage repair but usually results in fibrocartilage. Microfracture augmented by autologous bone marrow concentrate (BMC) was previously shown to yield structurally superior cartilage repairs in an equine model compared with microfracture alone. The current study was performed to test the hypothesis that autologous BMC without concomitant microfracture improves cartilage repair compared with microfracture alone. METHODS: Autologous sternal bone marrow aspirate (BMA) was concentrated using a commercial system...
January 17, 2018: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/29337871/influence-of-kartogenin-on-chondrogenic-differentiation-of-human-bone-marrow-derived-mscs-in-2d-culture-and-in-co-cultivation-with-oa-osteochondral-explant
#4
Timea Spakova, Jana Plsikova, Denisa Harvanova, Marek Lacko, Stefan Stolfa, Jan Rosocha
Articular cartilage has limited capacity for natural regeneration and repair. In the present study, we evaluated kartogenin (KGN), a bioactive small heterocyclic molecule, for its effect on in vitro proliferation and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells (hBMSCs) in monolayer culture and in co-culture models in vitro. OA osteochondral cylinders and hBMSCs were collected during total knee replacement. The effect of KGN on hBMSCs during 21 days of culture was monitored by real-time proliferation assay, immunofluorescence staining, histological assay, scanning electron microscopy (SEM) (imaging and multiplex enzyme-linked immunosorbent assay) ELISA assay...
January 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29337200/mimicking-the-3d-biology-of-osteochondral-tissue-with-microfluidic-based-solutions-breakthroughs-towards-boosting-drug-testing-and-discovery
#5
REVIEW
Mariana R Carvalho, Rui Luís Reis, Joaquim Miguel Oliveira
The development of tissue-engineering (TE) solutions for osteochondral (OC) regeneration has been slowed by technical hurdles related to the recapitulation of their complex and hierarchical architecture. OC defects refer to damage of both the articular cartilage and the underlying subchondral bone. To repair an OC tissue defect, the complexity of the bone and cartilage must be considered. To help achieve this, microfluidics is converging with TE approaches to provide new treatment possibilities. Microfluidics uses precise micrometer-to-millimeter-scale fluid flows to achieve high-resolution and spatial and/or temporal control of the cell microenvironment, providing powerful tools for cell culturing...
January 11, 2018: Drug Discovery Today
https://www.readbyqxmd.com/read/29331090/enhanced-chondrogenesis-of-mesenchymal-stem-cells-over-silk-fibroin-chitosan-chondroitin-sulfate-three-dimensional-scaffold-in-dynamic-culture-condition
#6
Parinita Agrawal, Krishna Pramanik, Varshini Vishwanath, Amit Biswas, Akalabya Bissoyi, Pradeep Kumar Patra
Chondroitin sulfate (Ch) is one of the main structural components of cartilage tissue, therefore, its presence in tissue engineered scaffold is expected to enhance cartilage regeneration. Previously, silk fibroin/chitosan (SF/CS) blend was proven to be a potential biomaterial for tissue development. In this study, the effect of Ch on physicochemical and biological properties of SF/CS blend was investigated and scaffolds with 0.8 wt% Ch was found to be favorable. The scaffolds possess pore size of 37-212 µm, contact angle 46...
January 13, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29329193/mustn1-a-developmentally-regulated-pan-musculoskeletal-cell-marker-and-regulatory-gene
#7
REVIEW
Michael Hadjiargyrou
The Mustn1 gene encodes a small nuclear protein (~9.6 kDa) that does not belong to any known family. Its genomic organization consists of three exons interspersed by two introns and it is highly homologous across vertebrate species. Promoter analyses revealed that its expression is regulated by the AP family of transcription factors, especially c-Fos, Fra-2 and JunD. Mustn1 is predominantly expressed in the major tissues of the musculoskeletal system: bone, cartilage, skeletal muscle and tendon. Its expression has been associated with normal embryonic development, postnatal growth, exercise, and regeneration of bone and skeletal muscle...
January 12, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29323151/downregulation-of-heat-shock-protein-70-impairs-osteogenic-and-chondrogenic-differentiation-in-human-mesenchymal-stem-cells
#8
Chenghai Li, Kristifor Sunderic, Steven B Nicoll, Sihong Wang
Human mesenchymal stem cells (hMSCs) show promise for bone and cartilage regeneration. Our previous studies demonstrated that hMSCs with periodic mild heating had enhanced osteogenic and chondrogenic differentiation with significantly upregulated heat shock protein 70 (HSP70). However, the role of HSP70 in adult tissue regeneration is not well studied. Here, we revealed an essential regulatory mechanism of HSP70 in osteogenesis and chondrogenesis using adult hMSCs stably transfected with specific shRNAs to knockdown HSP70...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29322878/mechanical-cellular-and-proteomic-properties-of-laryngotracheal-cartilage
#9
Christine M Pauken, Richard Heyes, David G Lott
The larynx sometimes requires repair and reconstruction due to cancer resection, trauma, stenosis, or developmental disruptions. Bioengineering has provided some scaffolding materials and initial attempts at tissue engineering, especially of the trachea, have been made. The critical issues of providing protection, maintaining a patent airway, and controlling swallowing and phonation, require that the regenerated laryngotracheal cartilages must have mechanical and material properties that closely mimic native tissue...
January 1, 2018: Cartilage
https://www.readbyqxmd.com/read/29317369/mesenchymal-stem-cell-derived-extracellular-matrix-enhances-chondrogenic-phenotype-of-and-cartilage-formation-by-encapsulated-chondrocytes-in-vitro-and-in-vivo
#10
Yuanheng Yang, Hang Lin, He Shen, Bing Wang, Guanghua Lei, Rocky S Tuan
Mesenchymal stem cell derived extracellular matrix (MSC-ECM) is a natural biomaterial with robust bioactivity and good biocompatibility, and has been studied as a scaffold for tissue engineering. In this investigation, we tested the applicability of using decellularized human bone marrow derived MSC-ECM (hBMSC-ECM) as a culture substrate for chondrocyte expansion in vitro, as well as a scaffold for chondrocyte-based cartilage repair. hBMSC-ECM deposited by hBMSCs cultured on tissue culture plastic (TCP) was harvested, and then subjected to a decellularization process to remove hBMSCs...
January 6, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29316655/nfatc1-is-a-functional-transcriptional-factor-mediating-nell-1-induced-runx3-upregulation-in-chondrocytes
#11
Chenshuang Li, Zhong Zheng, Xinli Zhang, Greg Asatrian, Eric Chen, Richard Song, Cymbeline Culiat, Kang Ting, Chia Soo
Neural EGFL like 1 (Nell-1) is essential for chondrogenic differentiation, maturation, and regeneration. Our previous studies have demonstrated that Nell-1's pro-chondrogenic activities are predominantly reliant upon runt-related transcription factor 3 (Runx3)-mediated Indian hedgehog (Ihh) signaling. Here, we identify the nuclear factor of activated T-cells 1 (Nfatc1) as the key transcriptional factor mediating the Nell-1 → Runx3 signal transduction in chondrocytes. Using chromatin immunoprecipitation assay, we were able to determine that Nfatc1 binds to the -833--810 region of the Runx3-promoter in response to Nell-1 treatment...
January 6, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29315262/intraarticularly-injected-mesenchymal-stem-cells-stimulate-anti-inflammatory-molecules-and-inhibit-pain-related-protein-and-chondrolytic-enzymes-in-a-monoiodoacetate-induced-rat-arthritis-model
#12
Toru Ichiseki, Miyako Shimazaki, Yoshimichi Ueda, Shusuke Ueda, Masanobu Tsuchiya, Daisuke Souma, Ayumi Kaneuji, Norio Kawahara
Persistent inflammation is well known to promote the progression of arthropathy. mesenchymal stem cells (MSCs) have been shown to possess anti-inflammatory properties and tissue differentiation potency. Although the experience so far with the intraarticular administration of mesenchymal stem cell (MSC) to induce cartilage regeneration has been disappointing, MSC implantation is now being attempted using various surgical techniques. Meanwhile, prevention of osteoarthritis (OA) progression and pain control remain important components of the treatment of early-stage OA...
January 9, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29313825/microspheres-containing-decellularized-cartilage-induce-chondrogenesis-i-in-vitro-i-and-remain-functional-after-incorporation-within-a-poly-caprolactone-filament-useful-for-fabricating-a-3d-scaffold
#13
Paulomi Ghosh, Stacey M S Gruber, Chia-Ying Lin, Patrick Whitlock
In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS+dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. The microspheres were spherical in shape and protein encapsulation efficiency within MS+dCM was 63.4%. The sustained release of proteins from MS+dCM was observed over 4 weeks in vitro. Both MS+dCM and MS(-) were cytocompatible...
January 9, 2018: Biofabrication
https://www.readbyqxmd.com/read/29313037/soluble-collagen-vi-treatment-enhances-mesenchymal-stem-cells-expansion-for-engineering-cartilage
#14
Piera Smeriglio, Jieun Lee, Nidhi Bhutani
Bone Marrow-derived mesenchymal stem cells (BM-MSC) are an attractive source for cell-based therapies in cartilage injury owing to their efficient differentiation into chondrocytes and their immune-suppressive abilities. However, their clinical use is hampered by a scarcity of cells leading to compromised efficacy. While expansion of human MSC ex vivo can potentially overcome the scarcity of cells, current methods lead to a rapid loss of the stem cell properties. In this study, we report soluble Collagen VI (cartilage pericellular matrix component) as a potential biologic that can expand the MSC population while maintaining the stem cell phenotype as confirmed by expression of the stem cell markers CD105 and CD90...
September 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/29312843/update-on-mesenchymal-stem-cell-therapies-for-cartilage-disorders
#15
EDITORIAL
Nikolaos K Paschos, Mackenzie L Sennett
Cartilage disorders, including focal cartilage lesions, are among the most common clinical problems in orthopedic practice. Left untreated, large focal lesions may result in progression to osteoarthritis, with tremendous impact on the quality of life of affected individuals. Current management strategies have shown only a modest degree of success, while several upcoming interventions signify better outcomes in the future. Among these, stem cell therapies have been suggested as a promising new era for cartilage disorders...
December 18, 2017: World Journal of Orthopedics
https://www.readbyqxmd.com/read/29310791/matrilins
#16
Mats Paulsson, Raimund Wagener
Marilins mediate interactions between macromolecular components of the extracellular matrix, e.g., collagens and proteoglycans. They are composed of von Willebrand factor type A and epidermal growth factor-like domains and the subunits oligomerize via coiled-coil domains. Matrilin-1 and -3 are abundant in hyaline cartilage, whereas matrilin-2 and -4 are widespread but less abundant. Mutations in matrilin genes have been linked to chondrodysplasias and osteoarthritis and recently characterization of matrilin-deficient mice revealed novel functions in mechanotransduction, regeneration, or inflammation...
2018: Methods in Cell Biology
https://www.readbyqxmd.com/read/29305998/effects-of-mechanical-loading-on-cortical-defect-repair-using-a-novel-mechanobiological-model-of-bone-healing
#17
Chao Liu, Robert Carrera, Vittoria Flamini, Lena Kenny, Pamela Cabahug-Zuckerman, Benson M George, Daniel Hunter, Bo Liu, Gurpreet Singh, Philipp Leucht, Kenneth A Mann, Jill A Helms, Alesha B Castillo
Mechanical loading is an important aspect of post-surgical fracture care. The timing of load application relative to the injury event may differentially regulate repair depending on the stage of healing. Here, we used a novel mechanobiological model of cortical defect repair that offers several advantages including its technical simplicity and spatially confined repair program, making effects of both physical and biological interventions more easily assessed. Using this model, we showed that daily loading (5N peak load, 2Hz, 60 cycles, 4 consecutive days) during hematoma consolidation and inflammation disrupted the injury site and activated cartilage formation on the periosteal surface adjacent to the defect...
January 4, 2018: Bone
https://www.readbyqxmd.com/read/29305868/quality-assessment-of-cellular-and-tissue-based-products-using-liquid-chromatography-tandem-mass-spectrometry
#18
Ayako Tominaga, Masato Sato, Takumi Takahahshi, Eriko Toyoda, Kenichi Toyoda, Takashi Suzuki, Masatoshi Takahashi, Masahiko Watanabe, Ken Okazaki
We are currently conducting clinical research on cell sheets for cartilage regeneration. One issue with the future use of chondrocyte sheets as cellular and tissue-based products is quality assessment. Currently, chondrocyte sheets are evaluated using invasive methods that cannot be performed on every sheet produced. We report here on our liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique that allows the noninvasive assessment of every sheet using only 50 μl of culture medium. We found that LC-MS/MS could be used to confirm cell sheet viability through the measurement of glucose and glutamine uptake, to estimate extracellular matrix production by measuring serine consumption, to estimate cell kinetics by measuring cytidine and uracil concentrations, and to estimate melanoma inhibitory activity level by measuring pyridoxal concentration...
January 3, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29300225/articular-cartilage-injury-pathways-and-treatment-options
#19
Timothy M Simon, Douglas W Jackson
Articular cartilage injury and degeneration is a frequent occurrence in synovial joints. Treatment of these articular cartilage lesions are a challenge because this tissue is incapable of quality repair and/or regeneration to its native state. Nonoperative treatments endeavor to control symptoms, and include anti-inflammatory medication, viscosupplementation, bracing, orthotics, and activity modification. Techniques to stimulate the intrinsic repair (fibrocartilage) process include drilling, abrasion, and microfracture of the subchondral bone...
March 2018: Sports Medicine and Arthroscopy Review
https://www.readbyqxmd.com/read/29299226/mobility-disorders-and-pain-interrelations-that-need-new-research-concepts-and-advanced-clinical-commitments
#20
Sascha Sajer
This Perspective will discuss topics recently suggested by Prof. Helmut Kern, Vienna, Austria, to advance the research activities of his team, that is: Topic A, 10 years post RISE; Topic B, New research for new solutions on old research questions; Topic C, Working groups on nerve regeneration, training-parameters of seniors in different ages, muscle adaptation; and studies of connective tissue and cartilage. This Perspective summarizes some of the basic concepts and of the evidence-based tools for developing further translational research activities...
December 5, 2017: European Journal of Translational Myology
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