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Cartilage regeneration

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https://www.readbyqxmd.com/read/28421969/dystrophic-mineralization-of-costal-cartilage-in-hartley-guinea-pigs
#1
Alexandria M Schauer, Adrienne Schucker, Cathy S Carlson
Hartley guinea pigs are widely used animal models of disease, particularly in studies of osteoarthritis. The purpose of this study was to investigate lesions in the costal cartilage from 16 male, 5- to 6-month-old Hartley guinea pigs. Routine histological sections from the costal cartilage and costochondral junction (longitudinal and cross sections) and sternum (for evaluation of bone marrow) were examined. All 16 (100%) animals had histological lesions involving the costal cartilage that included matrix degeneration and mineralization, reduced cellularity, and evidence of chondrocyte necrosis...
April 2017: Toxicologic Pathology
https://www.readbyqxmd.com/read/28417917/minicircle-mediated-gene-delivery-to-canine-and-equine-mesenchymal-stem-cells
#2
Naomie Tidd, Jacob Michelsen, Bryan Hilbert, Jane C Quinn
Gene-directed tissue repair offers the clinician, human or veterinary, the chance to enhance cartilage regeneration and repair at a molecular level. Non-viral plasmid vectors have key biosafety advantages over viral vector systems for regenerative therapies due to their episomal integration however, conventional non-viral vectors can suffer from low transfection efficiency. Our objective was to identify and validate in vitro a novel non-viral gene expression vector that could be utilized for ex vivo and in vivo delivery to stromal-derived mesenchymal stem cells (MSCs)...
April 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28414616/biphasic-finite-element-modeling-reconciles-mechanical-properties-of-tissue-engineered-cartilage-constructs-across-testing-platforms
#3
Gregory R Meloni, Matthew B Fisher, Brendan D Stoeckl, George R Dodge, Robert L Mauck
Cartilage tissue engineering is emerging as a promising treatment for osteoarthritis, and the field has progressed toward utilizing large animal models for proof of concept and preclinical studies. Mechanical testing of the regenerative tissue is an essential outcome for functional evaluation. However, testing modalities and constitutive frameworks used to evaluate in vitro grown samples differ substantially from those used to evaluate in vivo derived samples. To address this, we developed finite element (FE) models (using FEBio) of unconfined compression and indentation testing, modalities commonly used for such samples...
April 14, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28411451/anisotropic-tough-double-network-hydrogel-from-fish-collagen-and-its-spontaneous-in%C3%A2-vivo-bonding-to-bone
#4
Md Tariful Islam Mredha, Nobuto Kitamura, Takayuki Nonoyama, Susumu Wada, Keiko Goto, Xi Zhang, Tasuku Nakajima, Takayuki Kurokawa, Yasuaki Takagi, Kazunori Yasuda, Jian Ping Gong
Soft supporting tissues in the human body, such as cartilages and ligaments, are tough materials and firmly fixed to bones. These soft tissues, once injured, cannot regenerate spontaneously invivo. Developing tough and biocompatible hydrogels as artificial soft supporting tissues would substantially improve outcomes after soft tissue injury. Collagen is the main rigid component in soft connective tissues which is organized in various hierarchical arrays. We have successfully developed a novel class of collagen fibril-based tough hydrogels based on the double network (DN) concept using swim bladder collagen (SBC) extracted from Bester sturgeon fish...
April 5, 2017: Biomaterials
https://www.readbyqxmd.com/read/28401159/management-of-hepple-stage-v-osteochondral-lesion-of-the-talus-with-a-platelet-rich-plasma-scaffold
#5
Wenqi Gu, Tanzhu Li, Zhongmin Shi, Guohua Mei, Jianfeng Xue, Jian Zou, Xiaokang Wang, Haotong Zhang, Hongwei Xu
There has been no consensus on the treatment or prognosis of Hepple stage V osteochondral lesions of the talus (OLTs), especially for lesions greater than 1.5 cm(2) in size. The objective of this study was to investigate the clinical outcomes achieved upon application of a platelet-rich plasma (PRP) scaffold with a cancellous bone autograft for Hepple stage V OLTs. Fourteen patients (mean age, 39 years) were treated with a cancellous bone graft and a PRP scaffold between 2013 and 2015. The mean time to surgical treatment was 23...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28396682/heterologous-fibrin-sealant-derived-from-snake-venom-from-bench-to-bedside-an-overview
#6
REVIEW
Rui Seabra Ferreira, Luciana Curtolo de Barros, Luciana Patrícia Fernandes Abbade, Silvia Regina Catharino Sartori Barraviera, Maria Regina Cavariani Silvares, Leticia Gomes de Pontes, Lucilene Delazari Dos Santos, Benedito Barraviera
Hemostatic and adhesive agents date back to World War II, when homologous fibrin sealant came onto scene. Considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. Its components were a serine protease (a thrombin-like enzyme) extracted from the venom of Crotalus durissus terrificus snakes and a fibrinogen-rich cryoprecipitate extracted from the blood of Bubalus bubalis buffaloes. This new bioproduct has been used as a coagulant, sealant, adhesive and recently as a candidate scaffold for mesenchymal stem cells and bone and cartilage repair...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28396584/a-high-glucose-diet-affects-achilles-tendon-healing-in-rats
#7
Stefanie Korntner, Nadja Kunkel, Christine Lehner, Renate Gehwolf, Andrea Wagner, Peter Augat, Daniel Stephan, Verena Heu, Hans-Christian Bauer, Andreas Traweger, Herbert Tempfer
Chronic and acute tendinopathies are difficult to treat and tendon healing is generally a very slow and incomplete process and our general understanding of tendon biology and regeneration lags behind that of muscle or bone. Although still largely unexplored, several studies suggest a positive effect of nutritional interventions on tendon health and repair. With this study, we aim to reveal effects of a high-glucose diet on tendon neoformation in a non-diabetic rat model of Achilles tenotomy. After surgery animals received either a high-glucose diet or a control diet for 2 and 4 weeks, respectively...
April 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28393539/autologous-bone-marrow-cell-stimulation-and-allogenic-chondrocyte-implantation-for-the-repair-of-full-thickness-articular-cartilage-defects-in-a-rabbit-model
#8
Sungwook Choi, Gyeong Min Kim, Young Hee Maeng, Hyunseong Kang, Chen Tai Teong, Emily E Lee, Seung Jin Yoo, Darryl D Dlima, Myung Ku Kim
OBJECTIVE: The aim of this study was to evaluate the results of autologous bone marrow cell stimulation and allogenic chondrocyte implantation using 3-dimensional gel-type fibrin matrix in an animal model. DESIGN: Eighteen rabbits were divided into 2 treatment groups. One group was treated with a microfracture and covering of it with gel-type fibrin (AutoBMS; n = 9), and the other group was treated with allogenic chondrocytes mixed gel-type fibrin at the cartilage defect (AlloCI; n = 9)...
April 1, 2017: Cartilage
https://www.readbyqxmd.com/read/28387995/recent-strategies-in-cartilage-repair-a-systemic-review-of-the-scaffold-development-and-tissue-engineering
#9
REVIEW
Vikrant Rai, Matthew F Dilisio, Nicholas E Dietz, Devendra K Agrawal
Osteoarthritis results in irreparable loss of articular cartilage. Due to its avascular nature and low mitotic activity, cartilage has little intrinsic capacity for repair. Cartilage loss leads to pain, physical disability, movement restriction, and morbidity. Various treatment strategies have been proposed for cartilage regeneration, but the optimum treatment is yet to be defined. Tissue engineering with engineered constructs aimed towards developing a suitable substrate may help in cartilage regeneration by providing the mechanical, biological and chemical support to the cells...
April 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28386539/non-myogenic-contribution-to-muscle-development-and-homeostasis-the-role-of-connective-tissues
#10
REVIEW
Sonya Nassari, Delphine Duprez, Claire Fournier-Thibault
Skeletal muscles belong to the musculoskeletal system, which is composed of bone, tendon, ligament and irregular connective tissue, and closely associated with motor nerves and blood vessels. The intrinsic molecular signals regulating myogenesis have been extensively investigated. However, muscle development, homeostasis and regeneration require interactions with surrounding tissues and the cellular and molecular aspects of this dialogue have not been completely elucidated. During development and adult life, myogenic cells are closely associated with the different types of connective tissue...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28385649/prospects-of-sirna-applications-in-regenerative-medicine
#11
REVIEW
Fatemeh Mottaghitalab, Ali Rastegari, Mehdi Farokhi, Rassoul Dinarvand, Hossein Hosseinkhani, Keng-Liang Ou, Daniel W Pack, Chuanbin Mao, Meshkat Dinarvand, Yousef Fatahi, Fatemeh Atyabi
Small interfering RNA (siRNA) has established its reputation in the field of tissue engineering owing to its ability to silence the proteins that inhibit tissue regeneration. siRNA is capable of regulating cellular behavior during tissue regeneration processes. The concept of using siRNA technology in regenerative medicine derived from its ability to inhibit the expression of target genes involved in defective tissues and the possibility to induce the expression of tissue-inductive factors that improve the tissue regeneration process...
April 4, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28385238/self-crosslinking-and-injectable-hyaluronic-acid-rgd-functionalized-pectin-hydrogel-for-cartilage-tissue-engineering
#12
Feng Chen, Yunzhou Ni, Bing Liu, Tongtong Zhou, Chunyang Yu, Yue Su, Xinyuan Zhu, Xiaowei Yu, Yongfeng Zhou
In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides. The hydrazone crosslinking strategy is simple, while circumventing toxicity, making this injectable system feasible, minimally invasive and easily translatable for regenerative purposes. By varying their weight ratios, the physicochemical properties of the mechanically stable hydrogel system were easily adjustable...
June 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28385124/mimicking-cartilage-tissue-zonal-organization-by-engineering-tissue-scale-gradient-hydrogels-as-3d-cell-niche
#13
Danqing Zhu, Xinming Tong, Pavin Trinh, Fan Yang
Zonal organization plays an important role in cartilage structure and function, whereas most tissue-engineering strategies developed to date have only allowed the regeneration of cartilage with homogeneous biochemical and mechanical cues. To better restore tissue structure and function, there is a strong need to engineer materials with biomimetic gradient niche cues that recapitulate native tissue organization. To address this critical unmet need, here we report a method for rapid formation of tissue-scale gradient hydrogels as a 3D cell niche with tunable biochemical and physical properties...
April 6, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28375818/chondrocyte-attachment-proliferation-and-differentiation-on-three-dimensional-polycaprolactone-fumarate-scaffolds
#14
Eric R Wagner, Joshua Parry, Mahrokh Dadsetan, Dalibel Bravo, Scott M Riester, Andre J van Wijnen, Michael J Yaszemski, Sanjeev Kakar
Current treatment options for cartilage injuries are limited. The goals of this study are to create a biodegradable polymer scaffold with the capabilities of sustaining chondrocyte growth and proliferation, enable cell-to-cell communication and tissue regeneration through large pores, and assess the biological augmentation of the scaffold capabilities using platelet lysate (PL). We synthesized biodegradable polycaprolactone fumarate (PCLF) scaffolds to allow cell-cell communication through large interconnected pores...
March 31, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28369866/stem-cells-applications-in-bone-and-tooth-repair-and-regeneration-new-insights-tools-and-hopes
#15
Eiman Abdel Meguid, Yuehai Ke, Junfeng Ji, Ahmed Hk El-Hashash
The exploration of stem and progenitor cells holds promise for advancing our understanding of the biology of tissue repair and regeneration mechanisms after injury. This will also help in the future use of stem cell therapy for the development of regenerative medicine approaches for the treatment of different tissue-specie defects or disorders such as bone, cartilages and tooth defects or disorders. Bone is a specialized connective tissue, with mineralized extracellular components that provide bones with both strength and rigidity, and thus enable bones to function in body mechanical supports and necessary locomotion process...
March 30, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28365382/mapping-the-secretome-of-human-chondrogenic-progenitor-cells-with-mass-spectrometry
#16
Sarah Batschkus, Ilian Atanassov, Christof Lenz, Philipp Meyer-Marcotty, Gökhan Cingöz, Christian Kirschneck, Henning Urlaub, Nicolai Miosge
Tissue engineering offers promising perspectives in the therapy of osteoarthritis. In the context of cell-based therapy, chondrogenic progenitor cells (CPCs) may be used to regenerate defects in cartilage tissue. An in-depth characterization of the secretome of CPCs is a prerequisite to this approach. In this study, a method was developed for the qualitative and quantitative analysis of the secretome of undifferentiated and differentiated CPCs. Secreted proteins from cells grown in two-dimensional as well as three-dimensional alginate cultures were extracted and analyzed by liquid chromatography/tandem mass spectrometry (LC-MS/MS)...
March 30, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28359897/chitosan-hyaluronic-acid-hydrogel-for-cartilage-repair
#17
Neethu Mohan, P V Mohanan, A Sabareeswaran, Prabha Nair
The current study investigates the potential of chitosan-hyaluronic acid dialdehyde hydrogels for in vivo cartilage regeneration following two different approaches: Gel alone for cartilage regeneration or combination of chondrocytes and gel for cartilage repair. Critical size osteochondral defects were created in knee joints of Newzealand White rabbits. Allogenic rabbit chondrocytes were encapsulated in hydrogels and gel or gel+cells were implanted in defects aseptically. The regenerated cartilage was analyzed after 12 weeks of implantation...
March 27, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28359841/harnessing-camp-signaling-in-musculoskeletal-regenerative-engineering
#18
REVIEW
Okechukwu Clinton Ifegwu, Guleid Awale, Komal Rajpura, Kevin W-H Lo, Cato T Laurencin
Several emerging small molecules have been established for the repair or regeneration of musculoskeletal tissues. Cyclic AMP (cAMP) and its analogs have been the purview of many researchers in that they have been implicated in the repair or regeneration of several musculoskeletal tissues, such as bone, muscle, ligament, cartilage, blood vessels, skin, and nerve. Owing to the varied signal transduction pathways associated with cAMP, it elicits several processes and is thought to either activate or enhance native growth factors, thereby circumventing the need for supraphysiological doses of therapeutic growth factors in musculoskeletal tissue regeneration...
March 27, 2017: Drug Discovery Today
https://www.readbyqxmd.com/read/28359676/transplantation-of-the-decellularized-tracheal-allograft-in-animal-model-rabbit
#19
Reza Ershadi, Mohamadbagher Rahim, Siavash Jahany, Siamak Rakei
BACKGROUND: It has been difficult to perform tracheal allotransplantation without immunosuppression. To determine whether decellularized trachea can be used in tracheal replacement, we evaluated the viability of decellularized tracheal allografts in a rabbit model of immunosuppressant-free transplantation. METHOD: Half allograft (Group 1, n = 7) was harvested from adult New Zealand white rabbits, subjected to a detergent-enzymatic method (containing sodium deoxycholate/DNase lavations) of decellularization for as many cycles as needed, and the other half was stored in phosphate-buffered saline at 4°C as a control (Group 2, n = 7)...
March 27, 2017: Asian Journal of Surgery
https://www.readbyqxmd.com/read/28350666/long-term-comparison-between-human-normal-conchal-and-microtia-chondrocytes-regenerated-by-tissue-engineering-on-nanofiber-polyglycolic-acid-scaffolds
#20
Hitomi Nakao, Robin D Jacquet, Mark Shasti, Noritaka Isogai, Ananth S Murthy, William J Landis
BACKGROUND: Previous regeneration studies of auricle-shaped cartilage by tissue engineering leave unresolved whether the chondrocyte phenotype from human auricular chondrocytes seeded onto polymeric scaffolds is retained over the long term and whether microtia remnants may be a viable cell source for auricular reconstruction. METHODS: Chondrocytes were isolated from human ears, either normal conchal ear or microtia cartilage remnants, expanded in vitro, and seeded onto nanoscale-diameter polyglycolic acid sheets...
April 2017: Plastic and Reconstructive Surgery
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