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Novel Catechol O -methyltransferases from Lentinula edodes Catalyze the Generation of Taste-Active Flavonoids.
Journal of Agricultural and Food Chemistry 2024 May 4
Due to the increasing demand for natural food ingredients, including taste-active compounds, enzyme-catalyzed conversions of natural substrates, such as flavonoids, are promising tools to align with the principles of Green Chemistry. In this study, a novel O -methyltransferase activity was identified in the mycelium of Lentinula edodes , which was successfully applied to generate the taste-active flavonoids hesperetin, hesperetin dihydrochalcone, homoeriodictyol, and homoeriodictyol dihydrochalcone. Furthermore, the mycelium-mediated OMT activity allowed for the conversion of various catecholic substrates, yielding their respective (iso-)vanilloids, while monohydroxylated compounds were not converted. By means of a bottom-up proteomics approach, three putative O -methyltransferases were identified, and subsequently, synthetic, codon-optimized genes were heterologously expressed in Escherichia coli . The purified enzymes confirmed the biocatalytic O -methylation activity against targeted flavonoids containing catechol motifs.
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