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Journal of Agricultural and Food Chemistry

Jing Wang, Jiaxi Wang, Lu Zhang, Rutao Liu, Wansong Zong
This study investigated the adverse effects of sodium dodecylbenzene sulfonates (SDBS) on the mouse primary hepatocytes by conducting cell viability, intracellular oxidative stress level and catalase (CAT) activity assays. It was shown that SDBS altered CAT activities, triggered oxidative stress, and thus exhibited cytotoxicity to the hepatocytes. Both the stimulation of intracellular CAT production and the inhibition of molecular CAT activity contributed to intracellular CAT activity change. Molecular mechanisms underlying CAT activity inhibition and structural changes were explored by isothermal titration calorimetry, multi-spectroscopy and molecular docking studies...
March 24, 2017: Journal of Agricultural and Food Chemistry
David A Roeseler, Nancy J McGraw, Dustie N Butteiger, Naina Shah, Janine Hall-Porter, Ratna Mukherjea, Elaine Susan Krul
Dietary protein stimulates muscle protein synthesis and is essential for muscle health. We developed a screening assay using C2C12 mouse muscle cells to assess the relative abilities of diverse commercial proteins sources and experimental soy protein hydrolysates (ESH), after simulated gut digestion (SGD), to activate the mechanistic target of rapamycin complex I (mTORC1) muscle protein synthesis signaling pathway (p70S6K(Thr389) phosphorylation). Activation of mTORC1 was expressed as a percentage of a maximal insulin response...
March 24, 2017: Journal of Agricultural and Food Chemistry
Wu-Xia Bai, Chao Wang, Yijun Wang, Wen-Jun Zheng, Wei Wang, Xiaochun Wan, Guan-Hu Bao
A novel acylated flavonol tetraglycoside, kaempferol 3-O-[(E)-p- coumaroyl-(1→2)][α-L-arabinopyranosyl-(1→3)][β-D-glucopyranosyl (1→3)-α-L- rhamnopyranosyl (1→6)]-β-D-glucopyranoside (camellikaempferoside C, 1), together with two flavonol and eighteen flavone and flavonol glycosides (FGs) (2-21) was isolated from the green tea Lu'an GuaPian (Camellia sinensis L.O. Kuntze). Their structures were identified by spectroscopic and chemical methods. Four acylated FGs (1, 7, 8, 9) were found to inhibit the proliferation and differentiation of 3T3-L1 preadipocytes at the concentrations 25, 50, 100 µM (P < 0...
March 24, 2017: Journal of Agricultural and Food Chemistry
Tatiane Scilewski da Costa Zanatta, Roberta Manica-Berto, Cristiano Dietrich Ferreira, Michele Maciel Crizel Cardozo, Cesar Valmor Rombaldi, Rui Carlos Zambiazi, Álvaro Renato Guerra Dias
Phosphorus (P) intake, genotype, and growth environment in soybean cultivation can affect the composition of the soybean. This experiment was conducted in two locations (microregions I and II) using a randomized complete block design, including conventional soybean (BRS Sambaíba) and genetically modified (GM) [Msoy 9144 Roundup Ready (RR)] cultivars and varying doses of phosphorus fertilizer (0, 60, 120, and 240 kg/ha P2O5). Soybeans were evaluated for chemical composition, total phenols, phytic acid content, individual isoflavone content, antioxidant activity, oil quality, fatty acid profile, total carotenoid content, and individual tocopherol contents...
March 24, 2017: Journal of Agricultural and Food Chemistry
Jong Min Kim, Chang Hyeon Park, Seon Kyeong Park, Tae Wan Seung, Jin Yong Kang, Jeong Su Ha, Du Sang Lee, Uk Lee, Dae-Ok Kim, Ho Jin Heo
The ameliorating effects of ginsenoside Re (G Re) on high fat diet (HFD)-induced insulin resistance in C57BL/6 mice were investigated to assess its physiological function. In the results of behavioral tests, G Re improved cognitive dysfunction in diabetic mice using Y-maze, passive avoidance, and Morris water maze tests. G Re also significantly recovered hyperglycemia and fasting blood glucose level. In the results of serum analysis, G Re decreased triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDLC), glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) and increased the ratio of high-density lipoprotein cholesterol (HDLC)...
March 24, 2017: Journal of Agricultural and Food Chemistry
Tong Xing, Chong Wang, Xue Zhao, Chen Dai, Guanghong Zhou, Xinglian Xu
The current study was designed to investigate changes in the protein profiles of pale, soft, and exudative (PSE)-like muscles of broilers subjected to transportation under high-temperature conditions, using isobaric tags for relative and absolute analysis quantitation (iTRAQ). Arbor Acres chickens (n = 112) were randomly divided into two treatments: unstressed control (CON) and 0.5 h of transport (T). Birds were transported according to a designed protocol. Pectoralis major (PM) muscle samples in the T group were collected and classified as normal (T-NOR) or PSE-like (T-PSE)...
March 24, 2017: Journal of Agricultural and Food Chemistry
Giang Thanh Thi Ho, Eili Tranheim Kase, Helle Wangensteen, Hilde Barsett
Uptake of glucose and fatty acids in skeletal muscle is of interest for type 2 diabetes treatment. The aim was to study glucose and fatty acid uptake in skeletal muscle cells, antioxidant effects, and inhibition of carbohydrate-hydrolyzing enzymes by elderberries. Enhanced glucose and oleic acid uptake in human skeletal muscle cells were observed after treatment with phenolic elderberry extracts, anthocyanins, procyanidins, and their metabolites. The 96% EtOH and the acidified MeOH extracts were highly active...
March 24, 2017: Journal of Agricultural and Food Chemistry
Shun-Cheng Tseng, Tai-Shan Shen, Chia-Chieh Wu, Ing-Lin Chang, Hsin-Yao Chen, Chen-Pu Hsieh, Chun-Hsiang Cheng, Chiu-Liang Chen
Methyl protodioscin (MPD), a furostanol saponin derived from the rhizomes of Dioscorea collettii var. hypoglauca (Dioscoreaceae), has been shown to exhibit broad bioactivities such as anti-inflammation and antitumor activities. Here, we explored the molecular mechanisms by which MPD induced apoptosis in MG-63 cells. The data showed that MPD significantly suppressed cell growth (cell viabilities: 22.5 ± 1.9% for 8 μM MPD versus 100 ± 1.4% for control, P < 0.01) and enhanced cell apoptosis. The exposure to MPD resulted in a significant induction of reactive oxygen species, loss of mitochondrial membrane potential, and activation of caspase-9 and caspase-3 (P < 0...
March 24, 2017: Journal of Agricultural and Food Chemistry
Xuejuan Xia, Guannan Li, Yongbo Ding, Tingyuan Ren, Jiong Zheng, Jianquan Kan
This study investigated the hypolipidemic effect of whole grain Qingke (WGQ) and its influence on intestinal microbiota. Changes in the serum lipid, intestinal environment, and microbiota of Sprague-Dawley rats fed high-fat diets supplemented with different doses of WGQ were determined. Results showed that high doses of WGQ significantly decreased (P < 0.05) the Lee's index, serum total cholesterol, low-density lipoprotein cholesterol, and non-high-density lipoprotein cholesterol levels whereas they increased the body weight of the rats...
March 24, 2017: Journal of Agricultural and Food Chemistry
Jialin Wang, Yanfeng Hao, Hao Wang, Shaoxiang Yang, Hongyu Tian, Baoguo Sun, Yongguo Liu
The new fluorescent probe 4-hydroxy-3-((2E,4E)-5-phenylpenta-2,4-dienoyl)-2H-chromen-2-one (probe 1) was designed and synthesized for selective detection of sulfite. The fluorescence intensity of the probe was decreased only in the presence of HSO3(-); all other anions assessed resulted in an increased fluorescence response. Hence, probe 1 acts as a highly selective sensor for HSO3(-). This sulfite sensitivity can also be readily monitored visually, as once treated with sulfite the solution shows a marked color change from yellow to colorless...
March 24, 2017: Journal of Agricultural and Food Chemistry
Yinghui Li, Hongkui Wei, Fengna Li, Yehui Duan, Qiuping Guo, Yulong Yin
This study evaluated the effect of branched-chain amino acid (BCAA) supplementation in low-protein diets on lipid metabolism in dorsal subcutaneous adipose (DSA), abdominal subcutaneous adipose (ASA), and perirenal adipose (PRA) tissues. A total of 24 piglets were allotted to four treatments, and each group was fed the adequate protein (AP) diet, low-protein (LP) diet, LP diet supplemented with BCAA (LP + B), or LP diet supplemented with twice BCAA (LP + 2B). Serum concentrations of leptin in the BCAA-supplemented treatments were higher (P < 0...
March 24, 2017: Journal of Agricultural and Food Chemistry
Jean-Marc Brillouet, Hélène Fulcrand, Stéphanie Carrillo, Laurent Rouméas, Charles Romieu
Condensed tannins (also called proanthocyanidins) present in strategic tissues of fruits (outer pericarp and vascular bundles) were known as short polymers of flavan-3-ols. A pretreatment of the plant material (fruits from the grapevine, persimmon) with buffered ascorbic acid and Triton X-100 followed by acetone extraction provided native white fully depolymerizable tannins. Tannins are usually extracted with aqueous solvents and further purified, although artifactual oxidations occur, altering their physicochemical characteristics...
March 24, 2017: Journal of Agricultural and Food Chemistry
Julien Gabaston, Emma Cantos-Villar, Benoît Biais, Pierre Waffo-Teguo, Elodie Renouf, Marie-France Corio-Costet, Tristan Richard, Jean-Michel Mérillon
Stilbene-enriched extracts from Vitis vinifera waste (cane, wood, and root) were characterized by UHPLC-MS. Eleven stilbenes were identified and quantified as follows: ampelopsin A, (E)-piceatannol, pallidol, (E)-resveratrol, hopeaphenol, isohopeaphenol, (E)-ε-viniferin, (E)-miyabenol C, (E)-ω-viniferin, r2-viniferin, and r-viniferin. The fungicide concentration inhibiting 50% of growth of Plasmopara viticola sporulation (IC50) was determined for the extracts and also for the main compounds isolated. r-Viniferin followed by hopeaphenol and r2-viniferin showed low IC50 and thus high efficacy against Plasmopara viticola...
March 24, 2017: Journal of Agricultural and Food Chemistry
Benjamin W Redan, George P Albaugh, Craig S Charron, Janet A Novotny, Mario G Ferruzzi
As evidence mounts for a health-protective role of dietary phenolics, the importance of understanding factors influencing bioavailability increases. Recent evidence has suggested chronic exposure to phenolics may impact their absorption and metabolism. To explore alterations occurring from chronic dietary exposure to phenolics, Caco-2 cell monolayers were differentiated on Transwell inserts with 0-10 μM blackberry (Rubus sp.) total phenolics extracts rich in anthocyanins, flavonols, and phenolic acids. Following differentiation, apical to basolateral transport of phenolics was assessed from an acute treatment of 100 μM blackberry phenolics from 0 to 4 h...
March 24, 2017: Journal of Agricultural and Food Chemistry
José Manuel Muñoz-Redondo, Francisco Julián Cuevas, Juan Manuel León, Pilar Ramírez, José Manuel Moreno-Rojas, María José Ruiz-Moreno
A quantitative approach using HS-SPME-GC-MS was performed to investigate the ester changes related to the second fermentation in bottle. The contribution of the type of base wine to the final wine style is detailed. Furthermore, a discriminant model was developed based on ester changes according to the second fermentation (with 100% sensitivity and specificity values). The application of a double-check criteria according to univariate and multivariate analyses allowed the identification of potential volatile markers related to the second fermentation...
March 24, 2017: Journal of Agricultural and Food Chemistry
Veronika Mall, Peter Schieberle
In our previous study on the aroma compounds of heated prawn meat, the main odorants in blanched (BPM) and fried prawn meat (FPM), respectively, were characterized by means of gas chromatography-olfactometry and aroma extract dilution analysis. In this follow-up study, these aroma compounds were quantified by means of stable isotope dilution assays, and odor activity values (OAV; ratio of concentration to odor detection threshold) were calculated. Results revealed 2-acetyl-1-pyrroline and (Z)-1,5-octadien-3-one as the most potent odor-active compounds in both prawn samples...
March 24, 2017: Journal of Agricultural and Food Chemistry
Alice Giusti, Lara Tinacci, Carmen G Sotelo, Martina Marchetti, Alessandra Guidi, Wenjie Zheng, Andrea Armani
Few studies applying NGS have been conducted in the food inspection field, particularly on multispecies seafood products. A preliminary study screening the performance and the potential application in NGS analysis of 14 "universal primers" amplifying 16SrRNA, cytb, and COI genes in fish and cephalopods was performed. Species used in surimi preparation were chosen as target. An in silico analysis was conducted to test primers' coverage capacity by assessing mismatches (number and position) with the target sequences...
March 23, 2017: Journal of Agricultural and Food Chemistry
Carlos Jadán-Piedra, Marta Baquedano, Sergi Puig, Dinoraz Vélez, Vicenta Devesa
Food is the main pathway of exposure to inorganic mercury [Hg(II)] and methylmercury (CH3Hg). Intestinal absorption of these mercury species is influenced by their chemical form, the luminal pH, and the composition of the diet. In this regard, strategies have been proposed for reducing mercury absorption using dietary components. This study evaluates the capacity of Saccharomyces cerevisiae to reduce the amount of mercury solubilized after gastrointestinal digestion that is available for intestinal absorption (bioaccessibility)...
March 23, 2017: Journal of Agricultural and Food Chemistry
Yi Wu, Hao Cheng, Yantao Chen, Lingyun Chen, Zheng Fang, Li Liang
Clarification of the interaction mechanisms between proteins and bioactive components is important to develop effective carriers for encapsulation and protection of bioactive components. Bovine serum albumin (BSA), a globular protein in serum and milk, contains multiple sites to bind a variety of low-molecular-weight molecules, forming protein-mono-ligand complexes. In this study, the interactions of BSA with retinol, resveratrol and/or (-)-epigallocatechin-3-gallate (EGCG) were investigated by using fluorescence, circular dichroism and molecular docking techniques...
March 22, 2017: Journal of Agricultural and Food Chemistry
Fabricia Cristina Dos Reis, Valdemar Luiz Tornisielo, Rodrigo Floriano Pimpinato, Bianca A B Martins, Ricardo Victória Filho
When herbicides are applied in mixture, interactions among them could potentially promote changes in herbicide behavior in the soil. Thus, application mode (isolated or in mixture) and soil texture (sandy or clayey) were investigated in the total leaching of the commercial mixture diuron + hexazinone + sulfometuron-methyl, and of each isolated compound. Experiments in soil columns also evaluated the movement of each herbicide and mixtures across soil layers. In the sandy soil, the greatest total leaching was observed with hexazinone compared to diuron and sulfometuron...
March 22, 2017: Journal of Agricultural and Food Chemistry
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