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Journal of Agricultural and Food Chemistry

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https://www.readbyqxmd.com/read/29328677/metabolic-analysis-reveals-altered-long-chain-fatty-acid-metabolism-in-the-host-by-huanglongbing-disease
#1
Joon Hyuk Suh, Yue Niu, Zhibin Wang, Fred G Gmitter, Yu Wang
Candidatus Liberibacter asiaticus (CLas) is the presumed causal agent of Huanglongbing, one of the most destructive diseases in citrus. However, the lipid metabolism component of host response to this pathogen has not been investigated well. Here, metabolic profiling of a variety of long-chain fatty acids and their oxidation products was firstly performed to elucidate altered host metabolic responses of disease. Fatty acid signals were found to decrease obviously in response to disease regardless of cultivar...
January 12, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29328669/urinary-methyleugenol-deoxyadenosine-adduct-as-potential-biomarker-of-methyleugenol-exposure-in-rats
#2
Yukun Feng, Saide Wang, Hui Wang, Ying Peng, Jiang Zheng
Methyleugenol (ME), a natural ingredient of several herbs and spices used in the human diet, is hepatocarcinogenic in rodents. Following metabolic activation to reactive carbocation intermediate, ME can bind covalently to DNA, which is directly associated with its carcinogenicity. In this work, a non-invasive approach to determine ME exposure was established by monitoring urinary ME-dA adduct. The developed method entails liquid-liquid extraction enrichment of the urinary ME-dA, incorporation of deuterated ME-dA as internal standard, and analysis by liquid chromatography coupled tandem mass spectrometry...
January 12, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29328647/development-of-yam-dioscorin-loaded-nanoparticles-for-paracellular-transport-across-human-intestinal-caco-2-cell-monolayers
#3
Hung-Ling Hsieh, Chia-Hung Lee, Kuo-Chih Lin
Dioscorins, the major storage proteins of yam tubers, exert immunomodulatory activities. To improve the oral bioavailability of dioscorins in the intestine, recombinant dioscorin (rDioscorin) was coated with trimethyl chitosan (TMC) and tripolyphosphate (TPP), resulting in the formation of TMC-rDio-TPP nanoparticles (NPs). The loading capacity and entrapment efficiency of rDioscorin in the NPs were 26<u>+</u>0.7% and 61<u>+</u>1.4%, respectively. The NPs demonstrated a substantial release profile in the pH environment of the jejunum...
January 12, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29327928/one-step-bioconversion-of-fatty-acids-into-c8-c9-volatile-aroma-compounds-by-a-multifunctional-lipoxygenase-cloned-from-pyropia-haitanensis
#4
Zhu-Jun Zhu, Hai-Min Chen, Juanjuan Chen, Rui Yang, Xiao-Jun Yan
The multifunctional lipoxygenase PhLOX cloned from Pyropia haitanensis was expressed in E. coli with 24.4 mg•L-1 yield. PhLOX could catalyze the one-step bioconversion of C18-C22 fatty acids into C8-C9 volatile organic compounds (VOCs), displaying higher catalytic efficiency for eicosenoic and docosenoic acids than for octadecenoic acids. C20:5 was the most suitable substrate among the tested fatty acids. The C8-C9 VOCs were generated in good yields from fatty acids, e.g., 2E-nonenal from C20:4, and 2E,6Z-nonadienal from C20:5...
January 12, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29327584/gama-aminobutyric-acid-enriched-rice-bran-diet-attenuates-insulin-resistance-and-balances-energy-expenditure-via-modification-of-gut-microbiota-and-scfas
#5
Xu Si, Wenting Shang, Zhongkai Zhou, Guanghou Shui, Sin Man Lam, Christopher L Blanchard, Padraig Strappe
In this study, gama-aminobutyric acid (GABA) enriched rice bran (ERB) was supplemented to obese rats to investigate the attenuation of metabolic syndromes induced by high-fat diet. ERB-containing diet stimulated butyrate and propionate production by promoting Anaerostipes, Anaerostipes sp. and associated synthesizing enzymes. This altered SCFA distribution further enhanced circulatory levels of leptin and glucagon-like peptide-1, controlling food intake by down-regulating orexigenic factors. Together with the enhanced fatty acid β-oxidation highlighted by Prkaa2, Ppara, Scd1 expression via AMPK signaling pathway and Non-alcoholic fatty liver disease pathway, energy expenditure was positively modulated...
January 12, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29325417/rhubarb-supplementation-promotes-intestinal-mucosal-innate-immune-homeostasis-through-modulating-intestinal-epithelial-microbiota-in-goat-kids
#6
Jinzhen Jiao, Jian Wu, Min Wang, Chuanshe Zhou, Rong-Zhen Zhong, Zhi-Liang Tan
The abuse and misuse of antibiotics in livestock production pose a potential health risk globally. Rhubarb can serve as a potential alternative to antibiotics, and several studies have looked into its anti-cancer, anti-tumor and anti-inflammatory properties. The aim of this study was to test the effects of rhubarb supplementation to the diet of young ruminants on innate immune function and epithelial microbiota in the small intestine. Goat kids were fed with a control diet supplemented with or without rhubarb (1...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29325416/igy-reduces-afb1-induced-cytotoxicity-cellular-dysfunction-and-genotoxicity-in-human-l-02-hepatocytes-and-swan-71-trophoblasts
#7
Taotao Qiu, Xing Shen, Zhen Tian, Riming Huang, Xiangmei Li, Juan Wang, Rong Wang, Yuanming Sun, Yiguo Jiang, Huidong Zhang, Hongtao Lei
Aflatoxin B1 (AFB1) causes hepatotoxic, genotoxic, and immuno¬toxic effects in a variety of species. Although various neutralizing agents of AFB1 toxicity have been studied, the egg yolk immunoglobulins (IgY) detoxification of small molecular toxins and the mechanisms underlying such effects have not yet been reported. In this investigation, anti-AFB1 IgY against AFB1 was successfully raised, and a competitive indirect enzyme-linked immunosorbent assay was established with a sensitive half-maximal inhibitory concentration (IC50, 2...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29323924/akt-serine-threonine-kinase-1-regulates-de-novo-fatty-acid-synthesis-through-mtor-srebp1-axis-in-dairy-goat-mammary-epithelial-cells
#8
Tianying Zhang, Jiangtao Huang, Yongqing Yi, Xueying Zhang, Juan J Loor, Yanhong Cao, Huaiping Shi, Jun Luo
Akt serine/threonine kinase acts as a central mediator in the PI3K/Akt signaling pathway, regulating a series of biological processes. In lipid metabolism, Akt activation regulates a series of gene expressions, including genes related to intracellular fatty acid synthesis. However, the regulatory mechanisms of Akt in dairy goat mammary lipid metabolism have not been elaborated. In this study, the coding sequences of goat Akt1 gene were cloned and analyzed. Gene expression of Akt1 in different lactation stages was also investigated...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29323902/maillard-conjugation-of-sodium-alginate-to-whey-protein-for-enhanced-resistance-to-surfactant-induced-competitive-displacement-from-air-water-interfaces
#9
Bingqing Cai, Anna Saito, Shinya Ikeda
Whey protein adsorbed to an interface forms a viscoelastic interfacial film but is displaced competitively from the interface by a small-molecule surfactant added afterward. The present study evaluated the impact of the covalent conjugation of high- or low-molecular-weight sodium alginate (HA or LA) to whey protein isolate (WPI) via the Maillard reaction on the ability of whey protein to resist surfactant-induced competitive displacement from the air-water interface. Surfactant added after the pre-adsorption of conjugate to the interface increased surface pressure...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29323901/microbial-biosynthesis-of-silver-nanoparticles-in-different-culture-media
#10
Ke Luo, Samuel Jung, Kyu-Hwan Park, Young-Rok Kim
Microbial biosynthesis of metal nanoparticles has been extensively studied for the applications in biomedical sciences and engineering. However, the mechanism for their synthesis through microorganism is not completely understood. In this study, several culture media were investigated for their roles in the microbial biosynthesis of silver nanoparticles (AgNPs). The size and morphology of the synthesized AgNPs were analyzed by UV-Vis spectroscopy, FT-IR, TEM, and DLS. The results demonstrated that nutrient broth (NB) and Mueller-Hinton broth (MHB) among tested media effectively reduced silver ions to form AgNPs with different particle size and shape...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29323896/different-proteomic-processes-related-to-the-cultivar-dependent-cadmium-accumulation-of-amaranthus-gangeticus
#11
Chun-Tao He, Yihui Zhou, Ying-Ying Huang, Hui-Ling Fu, Xue-Song Wang, Fei-Yue Gong, Xiao Tan, Zhongyi Yang
To deal with the Cd-contaminant of agricultural soil, pollution-safe cultivar (PSC) is developed to minimize Cd accumulation risk in crops. The present study aimed to investigate the different proteomic responses related to Cd accumulation in different tissue between two Amaranthus gangeticus cultivars, Pen and Nan. Significant higher Cd accumulation in Pen than in Nan was unraveled, especially in shoot. The proportions of soluble Cd in root and stem of Nan were significantly lower than those of Pen, implying lower Cd transportation from root to shoot in Nan...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29323890/hrmas-nmr-and-mri-spectroscopies-distinguish-metabolome-and-structural-properties-of-maize-seeds-from-plants-treated-with-different-fertilizers-and-arbuscular-mycorrhizal-fungi
#12
Pierluigi Mazzei, Vincenza Cozzolino, Alessandro Piccolo
Both High-Resolution Magic-Angle-Spinning (HRMAS) and Magnetic Resonance Imaging (MRI) NMR spectroscopies were applied here to identify the changes of metabolome, morphology and structural properties induced in seeds (caryopses) of maize plants grown at field level under either mineral or compost fertilization in combination with the inoculation by Arbuscular Mycorrhizal Fungi (AMF). The metabolome of intact caryopses was examined by HRMAS-NMR, while the morphological aspects, endosperm properties and seed water distribution were investigated by MRI...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29323888/covalent-immobilization-of-cellulase-using-magnetic-poly-ionic-liquid-support-improvement-of-the-enzyme-activity-and-stability
#13
Seyed Hassan Hosseini, Seyedeh Ameneh Hosseini, Nasrin Zohreh, Mahshid Yaghoubi, Ali Pourjavadi
A magnetic nanocomposite was prepared by entrapment of Fe3O4 nanoparticles into the cross-linked ionic liquid/epoxy type polymer. The resulting support was used for covalent immobilization cellulase through the reaction with epoxy groups. The ionic surface of the support improved the adsorption of enzyme and a large amount of enzyme (106.1 mg/g) was loaded onto the support surface. The effect of the presence of ionic monomer and covalent binding of enzyme was also investigated. The structures of supports were characterized by various instruments such as FT-IR, TGA, VSM, XRD, TEM, SEM and DLS...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29322776/altered-serum-metabolite-profiling-and-relevant-pathways-analysis-in-rats-stimulated-by-honeybee-venom-a-new-insight-into-allergy-to-honeybee-venom
#14
Yazhou Zhao, Jianmei Zhang, Yanping Chen, Zhiguo Li, Hongyi Nie, Wenjun Peng, Songkun Su
To improve our understanding of the disturbed metabolic pathways and cellular responses triggered by honeybee venom stimulation, we compared the changes in serum metabolites rats, either stimulated or not by honeybee venom by performing 1H NMR spectrometry-based metabonomics to identify potential biomarkers. In this study, 65 metabolites were structurally confirmed and quantified and the following results were obtained. Firstly, by pattern recognition analysis, 14 metabolites were selected as potential biomarkers 3 hrs after venom stimulation...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29319312/induced-high-yield-production-of-zeaxanthin-lutein-and-%C3%AE-carotene-by-a-mutant-of-chlorella-zofingiensis
#15
Weiping Huang, Yan Lin, Mingxia He, Yuhao Gong, Junchao Huang
Natural resources of zeaxanthin are extremely limited. A Chlorella zofingiensis mutant (CZ-bkt1), which could accumulate high amounts of zeaxanthin, was generated and characterized. CZ-bkt1 was achieved by treating the algal cells with a chemical mutagen followed by a colour-based colony screening approach. CZ-bkt1 was found to consist of a dysfunctional carotenoid ketolase, leading to the accumulation of zeaxanthin rather than its downstream ketocarotenoid astaxanthin. Light irradiation, glucose, NaCl, and nitrogen deficiency all induced CZ-bkt1 to accumulate zeaxanthin...
January 10, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29276945/coffee-consumption-and-cardiovascular-disease-a-condensed-review-of-epidemiological-evidence-and-mechanisms
#16
Fernando Rodríguez-Artalejo, Esther López-García
Coffee is one of the most widely consumed beverages, and some studies have suggested it may be related to cardiovascular disease (CVD), the leading cause of poor health in the world. This review evaluates the evidence on the effect of habitual coffee consumption on CVD incidence and mortality. The review is based mostly on observational studies and meta-analyses of the literature. In healthy people, in comparison to not consuming coffee, habitual consumption of 3-5 cups of coffee per day is associated with a 15% reduction in the risk of CVD, and higher consumption has not been linked to elevated CVD risk...
January 10, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29314841/grape-berry-acclimation-to-excessive-solar-irradiance-leads-to-repartitioning-between-major-flavonoid-groups
#17
Noam Reshef, Nurit Agam, Aaron Fait
Warm viticulture regions are associated with inferior wines, resulting from the interaction between microclimate and fruit biochemistry. Solar irradiance triggers biosynthetic processes in the fruit, and dominates its thermal balance. Therefore, deciphering its impact on fruit metabolism is pivotal to develop strategies for fruit protection, and ameliorate its quality traits. Here, we modified light quality and intensity in the fruit-zone and integrated micrometeorology with grape and wine metabolomics, allowing a complete assessment, from field to bottle...
January 9, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29314836/utilization-of-a-genome-edited-tomato-solanum-lycopersicum-with-high-gamma-aminobutyric-acid-content-in-hybrid-breeding
#18
Jeongeun Lee, Satoko Nonaka, Mariko Takayama, Hiroshi Ezura
γ-Aminobutyric acid (GABA) is non-proteogenic amino acid with health-promoting functions. Although tomato fruits have a relatively high GABA content compared with other crops, levels must be further increased to effectively confer the health-promoting functions. In this study, we evaluated the potential of the genome-edited tomato as a breeding material for producing high-GABA hybrid tomatoes. Hybrid lines were produced by crossing the genome-edited tomato with a pure line tomato cultivar, 'Aichi First', and were evaluated for GABA accumulation and other fruit traits...
January 9, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29313353/simulated-digestion-and-fermentation-in-vitro-by-human-gut-microbiota-of-polysaccharides-from-bee-collected-pollen-of-chinese-wolfberry
#19
Wangting Zhou, Yamei Yan, Jia Mi, Hongcheng Zhang, Lu Lu, Qing Luo, Xiaoying Li, Xiaoxiong Zeng, Youlong Cao
Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study. It was found that WBPPS was mainly consisted of mannose, ribose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 0.38: 0.09: 0.17: 0.64: 0.22: 0.67: 0.08: 1.03, respectively. WBPPS was not affected by human saliva. The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded...
January 9, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29313349/analysis-of-the-effects-of-%C3%AE-tocopherol-%C3%AE-toh-on-raw264-7-and-k562-cells-based-on-sup-1-sup-h-nmr-metabonomics
#20
Yang Lu, Hui Li, Yue Geng
δ-Tocopherol (δ-TOH) is a form of vitamin E with higher bioactivity. In this study, we studied the bioactivity of δ-TOH using the IC50 of δ-TOH on RAW264.7 (80 μM) and K562 (110 μM) cells. We compared the differential metabolites from the cell lines with and without δ-TOH treatment by 1H-NMR metabonomics analysis. It was found that δ-TOH affected the protein biosynthesis, betaine metabolism, and urea cycle in various ways in both cell lines. Metabolic levels of the cell lines were changed after treatment with δ-TOH as differential metabolites were produced...
January 9, 2018: Journal of Agricultural and Food Chemistry
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