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Journal of Agricultural and Food Chemistry

Supatcha Kubglomsong, Chockchai- Theerakulkait, Ralph L Reed, Liping Yang, Claudia S Maier, Jan F Stevens
Rice bran albumin (RBAlb), which showed higher tyrosinase inhibitory activity than other protein fractions, was hydrolyzed with papain to improve the bioactivity. The obtained RBAlb hydrolysate (RBAlbH) was separated into 11 peptide fractions by RP-HPLC. Tyrosinase inhibition and copper chelation activities decreased with increasing retention time of the peptide fractions. RBAlbH fraction 1, which exhibited the greatest activity, contained 13 peptides whose sequences were determined by using LC-MS/MS. Most of the peptide sequences contained features of previously reported tyrosinase inhibitory and metal chelating peptides, especially peptide SSEYYGGEGSSSEQGYYGEG...
July 17, 2018: Journal of Agricultural and Food Chemistry
Man He, Yuming Zhou, Shuangxi Nie, Peng Lu, Huining Xiao, Yuan Tong, Qiang Liao, Ruili Wang
Two series of amphiphilic antimicrobial copolymers containing ciprofloxacin (CPF) and amine functional group have been synthesized via free radical copolymerization. The chemical structures of different amine groups and the copolymer composition have been systematically varied to study how structure of the copolymers exerted an influence on their antibacterial activity. The viability of Escherichia coli in the presence of antimicrobial copolymers was observed by confocal laser scanning microscopy (CLSM). CLSM as well as atomic force microscope (AFM) were applied to visualize changes in bacterial morphology treated with antimicrobial copolymers and discussed the antimicrobial mechanism of antimicrobial copolymers...
July 17, 2018: Journal of Agricultural and Food Chemistry
Xin-Sheng Qin, Zhigang Luo, Xichun Peng, Xuanxuan Lu, Yu-Xiao Zou
The development of multilayered interfacial engineering on the emulsion freeze-thaw properties has recently attracted widespread attention, because of the essential freeze-thaw storage process in some emulsion-matrix food products. In this research, we studied the role of salt concentration on the freeze-thaw properties of quinoa protein (QPI) nanoparticles stabilized Pickering emulsions. The QPI nanoparticles (particle concentration c=2%, w/v) with increasing particle size and surface hydrophobicity (H0) were fabricated by ultrasound treatment at 100 W for 20 min, by varying the NaCl addition (salt concentrations, 0-500 mM)...
July 17, 2018: Journal of Agricultural and Food Chemistry
Naihao Lu, Yinhua Sui, Lizeng Zeng, Rong Tian, Yiyuan Peng
The interactions between proteins and bioactive ligands (such as flavonoids, nanomaterials) are vital to design effective protein carriers for the protection of bioactive molecules and reduction of nanotubes' cytotoxicity. Bovine serum albumin (BSA) can bind various bioactive components and subsequently form protein-ligand complexes. Herein, the bindings of BSA to quercetin or single-walled carbon nanotubes (SWCNTs) were investigated by using experimental and molecular docking methods. The fluorescence intensity of BSA was decreased by both quercetin and SWCNTs in static quenching mode (i...
July 17, 2018: Journal of Agricultural and Food Chemistry
Hui-Hui Ren, You Fan, Bin Wang, Li-Ping Yu
In the present work, polyethyleneimine-capped CdS quantum dots (PEI-CdS QDs) with bright green fluorescence were synthesized and applied for sensitively and selectively detecting the nitrite in vegetable and water samples. Highly fluorescent and environment-friendly PEI-CdS QDs (quantum yield about 8%) with diameters of ca. 5 nm were easily synthesized by using hyperbranched PEI as functional polymer. Formation of the PEI-CdS QDs was verified by transmission electron microscopy and UV-vis spectroscopy. The fluorescence intensity of the as-synthesized PEI-CdS QDs was enhanced pronouncedly by the increasing amount of PEI and was stable when the pH ranged from 5...
July 17, 2018: Journal of Agricultural and Food Chemistry
Kai Chen, Zhinan Fu, Mingwei Wang, Yin Lv, Chunxin Wang, Yue Shen, Yan Wang, Haixin Cui, Xuhong Guo
Environmental concerns and low efficacy pose a challenge for application of traditional insecticide formulations. In this study, a series of lambda-cyhalothrin (LC) loaded nanoparticles (NPs) were produced by flash nanoprecipitation (FNP), and the parameters that influence the nanoparticle size were systematically studied. The narrow distribution and size-controllable NPs formed stable suspensions in aqueous solution without organic solvents. Amphiphilic block polymer PEG-PDLLA played an important role as a drug carrier, and the encapsulation content was as high as 99%...
July 17, 2018: Journal of Agricultural and Food Chemistry
Zahra Zahra, Seok-Young Kim, Hye-Youn Kim, Hwanhui Lee, Heayyean Lee, Jun-Yeong Jeon, Dong-Min Kim, Dong-Myung Kim, Seong-Joo Hong, Byung-Kwan Cho, Hookeun Lee, Choul-Gyun Lee, Muhammad Arshad, Hyung-Kyoon Choi
This study aimed to improve the production of phycobiliproteins using TiO2 nanoparticles (NPs) in Synechocystis sp. PCC 6803. The growth characteristics of Synechocystis cells were not affected by TiO2 NPs treatment, but this treatment increased the chlorophyll content significantly by 62.2% (14.6 mg/L) compared to that of control (9.0 mg/L) on day 16. Phycocyanin production was increased by 33.8% (29.3 g/L) compared to that of control (21.9 g/L) on day 8. Allophycocyanin production was increased by 55.0% (6...
July 17, 2018: Journal of Agricultural and Food Chemistry
Luigi Servillo, Nunzia D'Onofrio, Gianluca Neglia, Rosario Casale, Domenico Cautela, Massimo Marrelli, Antonio Limone, Giuseppe Campanile, Maria Luisa Balestrieri
Ruminants' milk contains δ-valerobetaine originating from rumen through the transformation of dietary Nε-trimethyllysine. Among ruminant's milk, the occurrence of δ-valerobetaine, along with carnitine precursors and metabolites, has not been investigate in buffalo milk, the second most worldwide consumed milk, well known for its nutritional value. HPLC-ESI-MS/MS analyses of bulk milk revealed that the Italian Mediterranean buffalo milk contains δ-valerobetaine at levels higher than bovine milk. Importantly, we detected also γ-butyrobetaine, the L-carnitine precursor, never described so far in any milk...
July 16, 2018: Journal of Agricultural and Food Chemistry
Meilin Li, Xiaoan Li, Jing Li, Yue Ji, Cong Han, Peng Jin, Yonghua Zheng
Strawberries were treated with different concentrations of ethanol vapor and then cut into four wedges and stored at 4 °C for one week. It was found that 4 mL/kg ethanol was the optimal concentration to reduce the decrease of firmness and weight loss. Total phenolics content, total flavonoid and anthocyanin contents, antioxidant enzyme activities and gene expression related to antioxidant were elevated by the ethanol treatment. Ethanol vapor also suppressed microbial growth and promoted free radical (hydroxyl and DPPH) scavenging capacities and four kinds of esters and bioactive components in strawberry wedges...
July 16, 2018: Journal of Agricultural and Food Chemistry
Fernando G Fermoso, Antonio Serrano, Bernabe Alonso-Fariñas, Juan Fernández-Bolaños, Rafael Borja, Guillermo Rodríguez
In a society where the environmental conscience is gaining attention, it is necessary to evaluate the potential valorisation options for agricultural biomass to create a change in the perception of the waste agricultural biomass from waste to resource. In that sense, the biorefinery approach has been proposed as the roadway to increase profit of the agricultural sector and, at the same time, ensure the environmental sustainability. The biorefinery approach integrates biomass conversion processes to produce fuels, power, and chemicals from biomass...
July 16, 2018: Journal of Agricultural and Food Chemistry
Kai Zhang, Meijuan Meng, Lipeng Gao, Yuanyu Tu, Yunfeng Bai
We investigated the effect of sodium butyrate feeding on the disruption of ruminal epithelium barrier function in goats fed a high-concentrate diet. Eighteen male Boer goats (live weight 31.75 ± 1.35 kg, aged 1 year) were randomly assigned to one of three groups, which were fed a low-concentrate diet (LC), a high-concentrate diet (HC), or a high-concentrate diet with 1% sodium butyrate by weight (SH) for 9 weeks. We found that the pH of rumen fluid in the SH and LC groups was higher than that in the HC group...
July 16, 2018: Journal of Agricultural and Food Chemistry
Baolei Jia, Zhongji Pu, Ke Tang, Xiaomeng Jia, Kyung Hyun Kim, Xinli Liu, Che Ok Jeon
D-lactate dehydrogenase (D-LDH) catalyzes the reversible reaction pyruvate + NADH + H+ <=> lactate + NAD+, which is a principal step in the production of D-lactate in lactic acid bacteria. In this study, we identified and characterized the major D-LDH (D-LDH1) from three D-LDHs in Leuconostoc mesenteroides, which has been extensively used in food processing. A molecular simulation study of D-LDH1 showed that the conformation changes during substrate binding. During catalysis, Tyr101 and Arg235 bind the substrates by hydrogen bonds and His296 acts as a general acid/base for proton transfer...
July 16, 2018: Journal of Agricultural and Food Chemistry
Sandeep Sharma, Line Degn Hansen, Jon Øvrum Hansen, Liv Torunn Mydland, Svein J Horn, Margareth Øverland, Vincent G H Eijsink, Kiira Susanna Vuoristo
The conversion of non-edible biomass to protein for use in feed is an attractive strategy towards improved sustainability in aquaculture. We have studied the possibility to produce the protein-rich yeast Candida utilis on a medium consisting of enzymatically hydrolysed sulphite-pulped spruce wood, mainly providing glucose, and enzymatically hydrolysed brown seaweed, supplemented with ammonium sulphate. The results show that this blend constitutes a complete medium that enables good growth rates and cell yields...
July 13, 2018: Journal of Agricultural and Food Chemistry
Shasha Li, Yucui Wu, Jing Kuang, Huaiqin Wang, Tangzhi Du, Yaya Huang, Yuan Zhang, Xiaoyan Cao, Zhezhi Wang
Transcription factors that include myeloblastosis (MYB), basic helix-loop-helix (bHLH), and WD-repeat protein, which often forms a ternary complex to regulate the phenylpropanoid pathway. However only a few MYB and bHLH members involved in the biosynthesis of salvianolic acid B (Sal B) have been reported, and little is known about Sal B pathway regulation by the WD40 protein transparent testa glabra1 (TTG1)-dependent transcriptional complexes in Salvia miltiorrhiza. We isolated SmTTG1 from that species for detailed functional characterization...
July 12, 2018: Journal of Agricultural and Food Chemistry
Kyung Hyun Kim, Xiaomeng Jia, Baolei Jia, Che Ok Jeon
One putative L-lactate dehydrogenase gene (L-ldh) and three putative D-ldh genes from Leuconostoc mesenteroides ATCC 8293 were overexpressed and their enzymatic properties were investigated. Only one gene showed D-LDH activity, catalyzing pyruvate and D-lactate interconversion, whereas other one and two genes displayed L- and D-malate dehydrogenase (MDH) activity (catalyzing oxaloacetate and L- and D-malate interconversion), respectively, suggesting that strain ATCC 8293 may not harbor an L-ldh gene. Putative phosphoenolpyruvate carboxylase (PEPC)- and malolactic enzyme (MLE)-encoding genes were identified from strain ATCC 8293, and sequence analysis showed that they could exhibit PEPC and MLE activities, respectively...
July 12, 2018: Journal of Agricultural and Food Chemistry
Jiwon Kim, Min-Yu Chung, Hee-Don Choi, In-Wook Choi, Byung Hee Kim
We enzymatically prepared structured monogalactosydiacylglycerols (MGDGs) enriched in pinolenic acid (PLA). PLA-enriched free fatty acids (FFAs) containing ~86 mol % PLA were produced from an FFA fraction obtained from pine nut oil (PLA content, ~13 mol %) by urea crystallization. Commercial MGDGs (5 mg) were acidolyzed with PLA-enriched FFAs using four commercial immobilized lipases as biocatalysts. The reaction was performed in acetone (4 mL) in a stirred-batch reactor. Lipozyme RM IM (immobilized Rhizomucor miehei lipase) was the most effective biocatalyst for the reaction...
July 12, 2018: Journal of Agricultural and Food Chemistry
Shupeng Yang, Huiyan Zhang, Marthe De Boevre, Jinzhen Zhang, Yanshen Li, Suxia Zhang, Sarah De Saeger, Jinhui Zhou, Yi Li, Feifei Sun
The lack of information on HT-2 toxin leads to the inaccurate hazard evaluation. In the present study, the toxicokinetic studies of HT-2 toxin were investigated following intravenous (i.v) and oral administration to rats at a dosage of 1.0 mg/kg body weight. After oral administration, HT-2 toxin was not detected in plasma, whereas its hydroxylated metabolite, 3'-OH HT-2 was identified. For i.v. administration, HT-2 toxin, its 3'-hydroxylated product and glucuronide derivative, 3-GlcA HT-2, were observed in plasma, among which the glucuronide conjugate was the predominant metabolite...
July 12, 2018: Journal of Agricultural and Food Chemistry
Ming Zhao, Jianjun Cheng, Brian Guo, Jinao Duan, Chun-Tao Che
Momilactones are allelochemicals in rice and moss defense. Momilactone-like compounds are therefore considered important secondary metabolites for plant defense. They may serve as promising lead compounds for crop-friendly herbicides, as well as anti-fungal and anti-bacterial agents. Many of these substances possess potent cytotoxicity property against cancer cell lines as well. The present paper is the first review on these versatile molecules, focusing on the structure, biological activity, chemical synthesis, and biosynthesis of the naturally occurring momilactone-like molecules reported from 1973 to 2017...
July 11, 2018: Journal of Agricultural and Food Chemistry
Tsui-Ling Chang, Pei-Shin Liou, Pei-Yuan Cheng, Hsiang-Ning Chang, Pei-Jane Tsai
Targeting the two degradation systems, ubiquitin-proteasome pathway and ubiquitin-signal autophagy-lysosome system, plays an important function in cancer prevention. Borneol is called an "upper guiding drug". Luteolin has demonstrated anti-cancer activity. That borneol regulates luteolin can be sufficient to serve as an alternative strategy. Borneol activates luteolin to inhibit E1 and 20S activity (IC50 = 118.8 ± 15.7 M) and perturb the 26S proteasome structure in vitro. Borneol regulates luteolin to inhibit 26S activity (IC50 = 157 ± 19 M), induces apoptosis (LC50 = 134 ± 4 M), and causes pre-G1 and G0/G1 arrest in HepG2 cells...
July 11, 2018: Journal of Agricultural and Food Chemistry
Danniele Miranda Bacila, Anildo Cunha, Indianara Fabíola Weber, Gerson Neudi Scheuermann, Arlei Coldebella, Luizinho Caron, Luciano Molognoni, Heitor Daguer, Luciana Igarashi Mafra, Vivian Feddern
Nicarbazin is one of the major anticoccidials used in broiler feeds. The compound 4,4´-dinitrocarbanilide (DNC) is the marker residue of concern left from nicarbazin in chicken meat. The effect of thermal processing on DNC content accumulated in chicken breast was assessed, and samples were analysed by liquid chromatography-tandem mass spectrometry. Five conventional cooking methods were evaluated: boiling, grilling, microwaving, frying, and roasting. To assure DNC in meat, broilers were fed nicarbazin without withdrawal period...
July 11, 2018: Journal of Agricultural and Food Chemistry
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