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Journal of Agricultural and Food Chemistry

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https://www.readbyqxmd.com/read/28635282/fining-of-red-wine-monitored-by-multiple-light-scattering
#1
Giovanna Ferrentino, Mohsen Ramezani, Ksenia Morozova, Daniela Hafner, Ulrich Pedri, Konrad Pixner, Matteo Scampicchio
This work describes a new approach based on multiple light scattering to study red wine clarification processes. The whole spectral signal (1933 backscattering points along the length of each sample vial) were fitted by a multivariate kinetic model that was built with a three-step mechanism, implying: 1) adsorption of wine colloids to fining agents; 2) aggregation into larger particles; 3) sedimentation. Each step is characterized by a reaction rate constant. According to the first reaction, the results showed that gelatin was the most efficient fining agent, concerning the main objective, which was the clarification of the wine, and consequently the increase in its limpidity...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28635280/anti-adhesive-activity-and-metabolomics-analysis-of-rat-urine-after-cranberry-vaccinium-macrocarpon-aiton-administration
#2
Gregorio Peron, Pellizzaro Anna, Brun Paola, Elisabetta Schievano, Stefano Mammi, Stefania Sut, Ignazio Castagliuolo, Stefano Dall'Acqua
Cranberry (Vaccinium macrocarpon Aiton) is used to treat non-complicated urinary tract infections (UTIs). A-type procyanidins (PAC-A) are considered the active constituents being able to inhibit bacterial adhesion to the urinary epithelium. However, there are doubts about PAC-A role in UTIs, due to their poor bioavailability, extensive metabolism, limited knowledge about urinary excretion and contradictory clinical trials. The effects of 35 days cranberry (11 mg/kg PAC-A, 4 mg/kg PAC-B) supplementation were studied in healthy rats using a UPLC-MS-based metabolomics approach...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28635270/understanding-the-effects-of-genotype-growing-year-and-breeding-on-tunisian-durum-wheat-allergenicity-part-2-the-celiac-disease-case
#3
Fatma Boukid, Barbara Prandi, Stefano Sforza, Rhouma Sayar, Yong Weon Seo, Mondher Mejri, Ines Yacoubi
The aim of this study was to compare immunogenic and toxic gluten peptides related to celiac disease (CD). 100 accessions of genotypes selected during the 20th century in Tunisia were in vitro digested, then analyzed by UPLC/ESI-MS technique using an isotopically labeled internal standard. The first MANOVA confirmed a high variability in the content of immunogenic and toxic peptides reflecting high genetic diversity in the germplasm released during the last century in Tunisia, consistently with PCA and clustering analysis results...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28635264/cadmium-accumulation-risk-in-vegetables-and-rice-in-southern-china-insights-from-solid-solution-partitioning-and-plant-uptake-factor
#4
Yang Yang, Meie Wang, Weiping Chen, Yanling Li, Chi Peng
Solid-solution partitioning coefficient (Kd) and plant uptake factor (PUF) largely determine the solubility and mobility of soil Cd to food crops. A four-year regional investigation was conducted in contaminated vegetable and paddy fields of southern China to quantify the variability in Kd and PUF. The distributions of Kd and PUF characterizing transfers of Cd from soil to vegetable and rice are probabilistic in nature. Dynamics in soil pH and soil Zn greatly affected the variations of Kd. In addition to soil pH, soil organic matter had a major influence on PUF variations in vegetables...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28635261/novel-protein-based-solid-biosensor-for-determining-pro-oxidant-activity-of-phenolic-compounds
#5
Esin Akyüz, Kevser Sözgen Başkan, Esma Tütem, Reşat Apak
To develop a protein‒based biosensor measuring the pro-oxidant activities of phenolic compounds, egg white proteins were precipitated with calcium chloride to obtain an insoluble calcium proteinate complex. This biosensor was used for the determination of Cu(II)‒induced pro-oxidant activity of antioxidants such as gallic acid, catechin, epicatechin, quercetin, chlorogenic acid and myricetin, and ascorbic acid. This assay involved the reduction of Cu(II) ions to Cu(I) by antioxidant compounds (simultaneously giving rise to reactive oxygen species) and binding of the formed Cu(I) to the solid biosensor...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28635260/development-validation-and-inter-laboratory-evaluation-of-a-quantitative-multiplexing-method-to-assess-levels-of-ten-endogenous-allergens-in-soybean-seed-and-its-application-to-field-trials-spanning-three-growing-seasons
#6
Ryan Christopher Hill, Trent J Oman, Xiujuan Wang, Guomin Shan, Barry Schafer, Rod A Herman, Rowel Tobias, Jeff Shippar, Bhaskar Malayappan, Li Sheng, Austin Xu, Jason Bradshaw
As part of the regulatory approval process in Europe, comparison of endogenous soybean allergen levels between genetically engineered (GE) and non-GE plants has been requested. A quantitative multiplex analytical method using tandem mass spectrometry has been developed and validated to measure ten potential soybean allergens from soybean seed. The analytical method was implemented at six laboratories to demonstrate the robustness of the method and further applied to three soybean field studies across multiple growing seasons (including 21 non-GE soybean varieties) to assess the natural variation of allergen levels...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28635255/characterization-and-immunomodulatory-activity-of-a-novel-peptide-ecfsta-from-wheat-germ-globulin
#7
Wenjia Wu, Mengmeng Zhang, Yao Ren, Xinze Cai, Zhina Yin, Xiaoai Zhang, Tian Min, Hui Wu
A novel peptide was extracted from wheat germ globulin and purified using ion-exchange chromatography, gel filtration chromatography and semi-preparative reverse phase-high performance liquid chromatography (RP-HPLC). The sequence of the peptide was found to be Glu-Cys-Phe-Ser-Thr-Ala (ECFSTA). Its immunomodulatory effects were evaluated, and the results showed that ECFSTA could enhance phagocytosis of RAW 264.7 cells and significantly increase their secretion of nitric oxide (NO), interleukins-6 (IL-6), tumor necrosis factors-α (TNF-α), and reactive oxygen species (ROS)...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28587447/identification-of-n-glycosites-in-chicken-egg-white-proteins-using-an-omics-strategy
#8
Fang Geng, Jinqiu Wang, Dayu Liu, Yongguo Jin, Meihu Ma
Chicken egg white (CEW) is a perfect source of natural proteins that possesses outstanding functional properties and various bioactivities. The glycosylation structure of CEW proteins plays important roles in their functions, bioactivities, and allergies. The present work attempted to identify N-glycosites of CEW proteins using an omics strategy. CEW proteins were digested with trypsin and chymotrypsin; glycopeptides were enriched and deglycosylated using PNGase F in H2(18)O water, followed by analysis using high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS)...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28562038/use-of-fe-impregnated-biochar-to-efficiently-sorb-chlorpyrifos-reduce-uptake-by-allium-fistulosum-l-and-enhance-microbial-community-diversity
#9
Xiao-Yan Tang, Wen-Da Huang, Jing-Jing Guo, Yang Yang, Ran Tao, Xu Feng
Fe-impregnated biochar was assessed as a method to remove the pesticide pollutant chlorpyrifos, utilizing biochar/FeOx composite synthesized via chemical coprecipitation of Fe(3+)/Fe(2+) onto Cyperus alternifolius biochar. Fe-impregnated biochar exhibited a higher sorption capacity than pristine biochar, resulting in more efficient removal of chlorpyrifos from water. Soil was dosed with pristine or Fe-impregnated biochar at 0.1 or 1.0% w/w, to evaluate chlorpyrifos uptake in Allium fistulosum L. (Welsh onion)...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28528547/differentiation-of-brazilian-peppertree-schinus-terebinthifolius-raddi-and-peruvian-peppertree-schinus-molle-l-fruits-by-uhplc-uv-ms-analysis-of-their-anthocyanin-and-biflavonoid-profiles
#10
Michelle M Feuereisen, Benno F Zimmermann, Nadine Schulze-Kaysers, Andreas Schieber
The objectives of this work were to determine the phenolic profile of Schinus terebinthifolius and Schinus molle fruits and to develop a reliable method for the differentiation of these two similar spices both known as pink pepper. Anthocyanins, biflavonoids and gallotannins, some of which are reported for the first time in these species, were identified by UHPLC-UV/vis-MS/MS. Consideration of the relative and absolute amounts of phenolics as well as indicator compounds from 18 samples revealed that the relative amounts of anthocyanins and biflavonoids are the most trustworthy parameters...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28631469/the-chemical-composition-of-blackberry-press-cake-polyphenolic-extract-and-defatted-seeds-and-their-effects-on-cecal-fermentation-bacterial-metabolites-and-blood-lipid-profile-in-rats
#11
Monika Kosmala, Adam Jurgonski, Jerzy Juskiewicz, Elżbieta Karlińska, Jakub Macierzyński, Edward Roj, Zenon Zduńczyk
Blackberry fiber (BF), extracted blackberry fiber (EBF), defatted blackberry seeds (DBS) and blackberry polyphenols (BP) were added to diets of rats. A control diet, fiber diets (BF, EBF, DBS) with 6% addition instead of cellulose, a polyphenol diet (BP) with 1% addition were administered for 4 weeks. BF and DBS contained polyphenols (3.6%), EBF did not. DBS was free of fat. Ellagitannins were the main phenolics; mostly dimers (sanguiin H-6 with isomers) and monomers (pedunculagin, casuarictin isomer, and sanguiin H-2 isomer)...
June 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28629219/thermally-induced-generation-of-desirable-aroma-active-compounds-from-the-glucosinolate-sinigrin
#12
Eva Ortner, Michael Granvogl
Glucosinolates are the most abundant secondary sulfur-containing plant metabolites in the plant family of Brassicaceae. These phytochemicals are well-known for their enzymatic degradation induced by the enzyme myrosinase resulting in pungent odor impressions derived from their respective degradation products. However, up to now, only little attention has been paid to non-enzymatic thermal degradation and the release of additional aroma-active compounds. Thermal treatment is particularly important in the processing of Brassica vegetables, and, thereby, glucosinolates as precursors can act as a natural source of odorants...
June 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28605187/phytotoxicity-study-on-bidens-sulphurea-sch-bip-as-a-preliminary-approach-for-weed-control
#13
Bruna P da Silva, Mariluce P Nepomuceno, Rosa M Varela, Ascensión Torres, José M G Molinillo, Pedro L C A Alves, Francisco A Macías
Farmers of the Franca region in Brazil observed that Bidens sulphurea was able to eliminate the Panicum maximum weed, which infected coffee plantations, without affecting the crop. In an effort to determine if the inhibitory effects observed were due to the presence of phytotoxic compounds from leaves and roots, a biodirected isolation and spectroscopic characterization has been carried out. The leaf dichloromethane and root acetone extracts were the most active, and the former appeared to be more phytotoxic to the target species, including four weeds...
June 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28602084/evaluation-of-the-interaction-between-pesticides-and-a-cell-membrane-model-by-surface-plasmon-resonance-spectroscopy-analysis
#14
Hiroshi Moriwaki, Kotaro Yamada, Hiromitsu Nakanishi
A surface plasmon resonance (SPR) spectroscopy analysis was used for the characterization of the interaction between pesticides and a cell membrane model. A liposome was immobilized onto the surface of the SPR sensor chip (L1), and the lipid bilayer membrane formed on the sensor chip was regarded as the cell membrane model. The solution containing a pesticide was flowed onto the sensor chip, and an SPR sensorgram, which reflected the interaction between the pesticide and the lipid bilayer membrane, was obtained...
June 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28571312/identification-of-indole-diterpenes-in-ipomoea-asarifolia-and-ipomoea-muelleri-plants-tremorgenic-to-livestock
#15
Stephen T Lee, Dale R Gardner, Daniel Cook
Ipomoea asarifolia has been associated with a tremorgenic syndrome in livestock in Brazil and was recently reported to contain tremorgenic indole diterpenes. Ipomoea muelleri has been reported to cause a similar tremorgenic syndrome in livestock in Australia. Ipomoea asarifolia and I. muelleri were investigated by high-performance liquid chromatography-high-resolution mass spectometry (HPLC-HRMS) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for indole diterpene composition...
June 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28627169/influence-of-the-fruit-juice-carriers-on-the-ability-of-lactobacillus-plantarum-dsm20205-to-improve-in-vitro-intestinal-barrier-integrity-and-its-probiotic-properties
#16
Estefanía Valero-Cases, Nicole Roy, Maria Jose Frutos, Rachel Anderson
This study investigates the influence of tomato and feijoa juices as fermentable carriers of Lactobacillus plantarum (LP DSM20205) on the ability of the bacterium to improve intestinal barrier function using the trans-epithelial electrical resistance (TEER) assay in an apical anaerobic model. The survival of LP DSM20205 in different fruit juices during in vitro digestion, its adhesion capacity and potential cytotoxic effect on Caco-2 cells were also studied. The results showed that carrier fruit juices have a significant influence on LP DSM20205 growth, survival during in vitro digestion, adhesion capacity and TEER...
June 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28627168/mitochondria-associated-apoptosis-in-human-melanoma-cells-induced-by-cardanol-monoene-from-cashew-nut-shell-liquid
#17
Wei-Chao Su, Yu-Feng Lin, Xiang-Ping Yu, Yu-Xia Wang, Xiao-Dong Lin, Qiao-Zhen Su, Dong-Yan Shen, Qing-Xi Chen
Cardanol monoene (CM) is the major phenolic component extracted from cashew nut shell liquid (CNSL), which has been relevant to wide range of biological effects. In this study, we found that CM could inhibit the M14 human melanoma cells proliferation in a dose dependent and time dependent manner, and the IC50 values were determined to be 23.15 ± 2.42 μM and 12.30 ± 1.67 μM after 24 h and 48 h treatment, respectively. The flow cytometric analysis demonstrated that CM induced M14 cell cycle arrest at S phase, along with the collapse of mitochondrial membrane potential (ΔΨm) and the accumulation of reactive oxygen species (ROS) level in cells,but the apoptotic cells reduced when treated with Z-VAD-FMK (pan-caspase inhibitor)...
June 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28627167/plasma-pharmacokinetics-bioavailability-and-tissue-distribution-of-four-c-glycosyl-flavones-from-mung-bean-vigna-radiata-l-seeds-extracts-in-rat-by-uhplc-ms-ms
#18
Yan Bai, Qili Zhang, Baoyu Wang, Meiyan Zhang, Yan Xu, Shasha Li, Yunli Zhao, Zhiguo Yu
The main polyphenols in mung bean (Vigna radiata L.) seeds (MBS), an edible legume with various biological activities, are C-glycosyl flavones (vitexin, isovitexin, isovitexin-6''-O-α-L-glucoside, and dulcinoside). In our study, a validated UHPLC-MS/MS method was developed to quantitate the concentrations of four C-glycosyl flavones from MBS extracts in the plasma and various tissues of rats, and successfully applied to study their pharmacological profile and tissue distribution in vivo. Four C-glycosyl flavones were rapidly absorbed after oral administration, achieving a Cmax at around 1...
June 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28597655/identification-and-characterization-of-3-epi-rotundone-a-novel-stereoisomer-of-rotundone-in-several-kinds-of-fruits
#19
Akira Nakanishi, Makiko Ito, Keisuke Yoshikawa, Tomoko Maeda, Susumu Ishizaki, Yoshiko Kurobayashi
A novel stereoisomer of rotundone, 3-epi-rotundone, was identified in the aroma of grapefruit, orange, apple, and mango. 3-epi-Rotundone was prepared by the isomerization of rotundone, and its structural elucidation was confirmed by comparing the 1D and 2D nuclear magnetic resonance and nuclear Overhauser effect spectroscopy spectra with those of rotundone. The odor thresholds of rotundone and 3-epi-rotundone in water were determined by a triangle test as 5 and 19100 ng/kg, respectively. The odor of 3-epi-rotundone was evaluated as woody, spicy, peppery, citrus, grapefruit-like, powdery, and celery-like, which was a greater range of odor characteristics than that for rotundone...
June 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28593759/enzymatic-synthesis-and-purification-of-galactosylated-chitosan-oligosaccharides-reducing-adhesion-of-enterotoxigenic-escherichia-coli-k88
#20
Ya Lu Yan, Ying Hu, David J Simpson, Michael G Gänzle
Enterotoxigenic Escherichia coli (ETEC) K88 causes diarrhea in weaned piglets and represent a suitable model system for ETEC causing childhood diarrhea. This study aimed to evaluate the effects of oligosaccharides against ETEC K88 adhesion to porcine erythrocytes with two bioassays. Galactosylated chitosan-oligosaccharides (Gal-COS) were synthesized through transgalactosylation by β-galactosidase. Fractions 2-5 of Gal-COS were obtained through cation exchange and size exclusion chromatography. Fractions 2-5 of acetylated Gal-COS were obtained through chemical acetylation followed by size exclusion chromatography...
June 19, 2017: Journal of Agricultural and Food Chemistry
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