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RNase H-dependent DNA thresholder modulated by cancer marker concentration.

Moustapha A Y Nour, Valeriia S Drozd, Evgenii A Lemeshko, Lilia Tafran, Adeliia A Salimova, Alexandra V Kulikova, Ahmed A Eldeeb
Chemical Communications : Chem Comm 2024 April 3
Threshold antisense oligonucleotide constructs were designed to cleave mRNA within different biomarker concentrations. The mRNA cleavage is activated by 2.6, 7.5 or 39.5 nM of biomarker depending on the construct design. The constructs can be used to differentiate cancer from normal cells by the level of oncogene expression followed by silencing of a targeted gene.

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