Plant mediated biosynthesis of Mn 3 O 4 nanostructures and their biomedical applications.

Nanomaterials have drawn significant attention for their biomedical and pharmaceutical applications. In the present study, manganese tetra oxide (Mn3 O4 ) nanoparticles were prepared greenly, and their physicochemical properties were studied. Taxus baccata acetone extract was used as a safely novel precursor for reducing and stabilizing nanoparticles. The synthesized nanoparticles were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) and X-ray diffraction (XRD). The cytotoxicity of Mn3 O4 (hausmannite) nanostructures was evaluated against murine macrophage cell line J774-A1 and U87 glioblastoma cancer cells for approximately 72 h. Spherical Mn3 O4 nanoparticles with tetragonal spinel structures demonstrated minimal toxicity against normal body cells with CC50 around 876.38 μg mL-1 . Moreover, Mn3 O4 nanoparticles as well as the combination of antimoniate meglumine and Mn3 O4 nanoparticles exhibited maximum mortality in Leishmania major . The synthesized nanominerals displayed a significant inhibitory effect against glioblastoma cancer cells at 100 μg mL-1 . The selective cytotoxicity of Mn3 O4 nanoparticles indicates that these biogenic agents can be employed simultaneously for diagnostic and therapeutic applications in medical applications.