Effect of a 14-Day Period of Heat Acclimation on Horses Using Heated Indoor Arenas in Preparation for Tokyo Olympic Games.

To optimise the performance and welfare of horses during equestrian competitions in hot climates, it is advised to acclimate them to the heat. The effects of training in a heated indoor arena were studied. Four Olympic horses (13.3 ± 2.2 years; three eventers, one para-dressage horse) were trained for 14 consecutive days in a heated indoor arena (32 ± 1 °C; 50-60% humidity) following their normal training schedule in preparation for the Tokyo Olympic games. Standardised exercise tests (SETs) were performed on Day 1 and Day 14, measuring heart rate (HR; bpm), plasma lactate concentration (LA; mmol/L), deep rectal temperature (Trec ; °C), sweat loss (SL; L), and sweat composition (K+ , Cl- and Na+ concentration). The data were analysed using linear mixed models. The Trec and HR were significantly decreased after acclimation (estimate: -0.106, 95% CI -0.134, -0.078; estimate: -4.067, 95% CI -7.535, -0.598, respectively). Furthermore, for all the horses, the time taken to reach their peak Trec and heat storage increased, while their LA concentrations decreased. The SL, Cl- , and Na+ concentrations decreased in three out of the four horses. Conclusions: Fourteen days of normal training in a heated indoor arena resulted in a reduction in cardiovascular and thermal strain. This is advantageous because it shows that elite sport horses can be acclimated while training as usual for a championship.