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Thin-section- and matrix-free mass spectrometry imaging: Reproducible sample transfer using novel platinum-coated porous plate formed of glass beads.
Rapid Communications in Mass Spectrometry : RCM 2024 March 31
RATIONALE: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) typically requires sample preparation such as sectioning and spraying of the matrix. Sample transfer using a Pt-coated porous plate formed of glass beads simplifies preparation and enables reproducible MSI measurements.
METHODS: The surface of a sintered-glass-bead porous plate was coated with Pt on one side of the plate. Polymer additives and the cross-section of a strawberry were chosen as the sample and transferred to the Pt-coated surface of the plate. This process was completely thin-section- and matrix-free. The prepared plates were analyzed using a commercial MALDI time-of-flight instrument.
RESULTS: Several constituents of the polymer additives (Irgafos 168 and Irganox 1010) and strawberry metabolites (hexose, citric acid, and sucrose) were detected without organic matrices. These ion images were obtained with a special distribution of the retained constituents. Duplicate prepared plates using the same strawberry cross-section reproduced the ion images.
CONCLUSIONS: A sample transfer process using a Pt-coated porous plate formed of glass beads was demonstrated as an alternative preparation method for MSI. This process has the potential to simplify MSI preparation and achieve easily reproducible MSI measurements.
METHODS: The surface of a sintered-glass-bead porous plate was coated with Pt on one side of the plate. Polymer additives and the cross-section of a strawberry were chosen as the sample and transferred to the Pt-coated surface of the plate. This process was completely thin-section- and matrix-free. The prepared plates were analyzed using a commercial MALDI time-of-flight instrument.
RESULTS: Several constituents of the polymer additives (Irgafos 168 and Irganox 1010) and strawberry metabolites (hexose, citric acid, and sucrose) were detected without organic matrices. These ion images were obtained with a special distribution of the retained constituents. Duplicate prepared plates using the same strawberry cross-section reproduced the ion images.
CONCLUSIONS: A sample transfer process using a Pt-coated porous plate formed of glass beads was demonstrated as an alternative preparation method for MSI. This process has the potential to simplify MSI preparation and achieve easily reproducible MSI measurements.
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