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Rapid Communications in Mass Spectrometry: RCM

Vladimír Vrkoslav, Barbora Rumlová, Timotej Strmeň, Pavlína Nekvasilová, Miloslav Šulc, Josef Cvačka
RATIONALE: Mass spectrometry with atmospheric pressure chemical ionization (APCI) or photoionization (APPI) is widely used for neutral lipids involved in many fundamental processes in living organisms. Commercial APCI and APPI sources operate at high flow rates compatible with conventional HPLC. However, lipid analysis is often limited by a small amount of sample, which requires low-flow rate separations like capillary or micro-HPLC. Therefore, APCI and APPI suitable for microliter-per-minute flow rates need to be developed and applied for neutral lipids...
February 18, 2018: Rapid Communications in Mass Spectrometry: RCM
Tiago Rosado, David Oppolzer, Belinda Cruz, Mário Barroso, Samira Varela, Victor Oliveira, Carlos Leitão, Eugenia Gallardo
RATIONALE: Antipsychotic drugs are prescription medications used to treat psychotic disorders, such as schizophrenia, schizoaffective disorder, or psychotic depression. With several antipsychotic drugs currently available all over the world, this class of drugs has quickly gotten importance in both clinical and forensic context. This work describes the development and validation of a methodology for the determination of seven antipsychotic drugs in plasma and oral fluid samples. METHODS: The antipsychotic drugs (chlorpromazine, clozapine, haloperidol, olanzapine, quetiapine, cyamemazine and, levomepromazine) were isolated from 0...
February 15, 2018: Rapid Communications in Mass Spectrometry: RCM
Allison A Baczynski, Pratigya J Polissar, Dieter Juchelka, Johannes Schwieters, Andreas Hilkert, Roger E Summons, Katherine H Freeman
RATIONALE: We report modifications to compound-specific isotope analyses (CSIA) to enable high-precision isotopic analysis of picomoles of carbon for intact organic molecules. This sample size is two orders of magnitude below the amounts required for commercial systems. The greatly enhanced sensitivity of this system expands molecular isotope studies and applications previously prohibited by low concentrations and small samples. METHODS: We utilize the resolving power and low volumetric flow rates of narrow-bore capillary GC to improve sample transfer efficiency while maintaining narrow peak widths...
February 15, 2018: Rapid Communications in Mass Spectrometry: RCM
Naoko Obi, Tetsuya Fukuda, Noboru Nakayama, John Ervin, Yasuhiko Bando, Toshihide Nishimura, Satoru Nagatoishi, Kouhei Tsumoto, Takeshi Kawamura
RATIONALE: Drug discovery studies invariably require qualitative and quantitative analyses of target compounds at every stage of drug discovery. We have developed a system combining molecular interaction analysis and mass spectrometry (LC-MS) using the principle of nano pore optical interferometry (nPOI) called molecular interaction kinetics-mass spectrometry (MIK-MS). Since nPOI has high binding capacity, the bond-dissociated compound can be directly detected using LC-MS. In this study, we use carbonic anhydrase (CA) II as a ligand and apply six small compounds as analytes and report the affinity analysis using MIK-MS...
February 14, 2018: Rapid Communications in Mass Spectrometry: RCM
Małgorzata Olejnik, Lidia Radko, Piotr Jedziniak
RATIONALE: Salinomycin is an ionophore antibiotic with potential anti-cancer activity. The history of its use in veterinary medicine show large differences in species susceptibility to its toxicity. At the same time, the results of so-far research suggest the correlation between the extent and pathways of ionophore biotransformation and its toxicity. The biotransformation pattern of salinomycin has not been studied so far. METHODS: The extracts from culture media of human hepatoma cells (HepG2) exposed to salinomycin were analysed with two mass spectrometry techniques...
February 14, 2018: Rapid Communications in Mass Spectrometry: RCM
H G M Eggenkamp, P Louvat
RATIONALE: In natural samples bromine is present in trace amounts, and measurement of Br stable isotopes necessitates its separation from the matrix. Most methods described previously need large samples or samples with high Br/Cl ratios. The use of metals as reagent, proposed in previous Br distillation methods, must be precluded for MC-ICP-MS analyses, because of risk of cross contamination, since the instrument is also used to measure stable isotopes of metals. METHODS: Dedicated to water and evaporite samples with low Br/Cl ratios, the proposed method is a simple distillation that separates bromide from chloride for isotopic analyses by MC-ICP-MS...
February 9, 2018: Rapid Communications in Mass Spectrometry: RCM
Jenna Johnston, Catherine Orrell, Peter Smith, Anton Joubert, Lubbe Wiesner
RATIONALE: Drug levels in hair provide a longer window of detection, compared to plasma drug levels, and therefore hair analysis has the advantage of assessing adherence over a longer period of time. No methods for the analysis of antiretroviral drugs in hair currently exist in South Africa, and worldwide there is only one validated method for the determination of efavirenz in hair that has been published. METHODS: Efavirenz was extracted from 0.2 mg of hair through a simultaneous pulverization and extraction step...
February 8, 2018: Rapid Communications in Mass Spectrometry: RCM
Edita Rados, Ernst Pittenauer, Johannes Frank, Kurt Varmuza, Günter Allmaier
RATIONALE: We have developed a target system which enables the use of only one target (i.e. target preparation set) for three different LDI/MALDI-MS instruments. The focus was on analyzing small biomolecules with LDI for future use of the system for the study of meteorite samples (carbonaceous chondrites) on devices with different mass spectrometric performance characteristics. METHODS: Three compounds were selected due to their potential presence in meteoritic chondrites: tryptophan, 2-deoxy-D-ribose and triphenylene...
February 8, 2018: Rapid Communications in Mass Spectrometry: RCM
Minghua Yang, Fang Zhang, Yuting Hou, Yue Su, Yinlong Guo
No abstract text is available yet for this article.
February 8, 2018: Rapid Communications in Mass Spectrometry: RCM
(no author information available yet)
No abstract text is available yet for this article.
February 8, 2018: Rapid Communications in Mass Spectrometry: RCM
An-Wen Kung, Peter M Kilby, David E Portwood, Mark J Dickman
RATIONALE: Recent developments in RNA interference (RNAi) have created a need for cost-effective and large scale synthesis of double stranded RNA (dsRNA), in conjunction with high throughput analytical techniques to fully characterise and accurately quantify dsRNA prior to downstream RNAi applications. METHODS: Stable isotope labeled dsRNA was synthesised both in vivo (15 N) and in vitro (13 C,15 N guanosine-containing dsRNA) prior to purification and quantification...
February 3, 2018: Rapid Communications in Mass Spectrometry: RCM
Joanna Dudkowska, Magdalena Frańska, Rafał Frański
RATIONALE: Cephalosporins (e.g. cephalexin, cefradine) are a major group of widely used β-lactam antibiotics. Hydrolysis of β-lactam ring is an important reaction (often undesired) which leads to β-lactams deactivation. To the best of our knowledge there is no ESI-MS data reported concerning the products of hydrolysis of cephalosporins. METHODS: The hydrolysis of cephalexin and cefradine was performed in NaOH water solutions. After the process the solutions were analyzed by HPLC-ESI/MS...
February 3, 2018: Rapid Communications in Mass Spectrometry: RCM
Yi Gong, Yunkai Li, Xinjun Chen, Ling Chen
RATIONALE: Squid is an important seafood resource for Asian and European countries. With the continuous development of processed squid products, an effective traceability system has become increasingly prominent. Here, we attempt to trace the fishery products of the main target species, jumbo squid (Dosidicus gigas), by using biochemical tracers. METHODS: Carbon and nitrogen isotope ratios (δ13 C and δ15 N values) and fatty acid profiles were identified in squid from three harvest locations in the eastern Pacific Ocean by isotope ratio mass spectrometry and gas chromatography/mass spectrometry, respectively...
February 3, 2018: Rapid Communications in Mass Spectrometry: RCM
Edward S Chen, Edward C M Chen
No abstract text is available yet for this article.
February 3, 2018: Rapid Communications in Mass Spectrometry: RCM
Chia-Wei Tsai, Christopher A Tipple, Richard A Yost
RATIONAL: Paper spray ionization (PSI) is an attractive ambient ionization source for mass spectrometry (MS) since it allows the combination of surface sampling and ionization. The minimal sample preparation inherent in this approach greatly reduces the time needed for analysis. However, the ions generated from interfering compounds in the sample and the paper substrate may interfere with the analyte ions. Therefore, the integration of PSI with high-field asymmetric ion mobility spectrometry (FAIMS) is of significant interest since it should reduce the background ions entering the mass analyzer without complicating the analysis or increasing analysis time...
January 30, 2018: Rapid Communications in Mass Spectrometry: RCM
Joshua N Bostic, William M Hagopian, A Hope Jahren
RATIONALE: Stable isotope analysis (SIA), a potential method of verifying the geographic origin and production method of dairy products, has not been applied to United States (U.S.) dairy samples on a national scale. To determine the potential of carbon and nitrogen SIA in authenticity assessment of U.S. dairy products, we analyzed a geographically-representative collection of conventional milk samples to determine isotopic variations with (1) Purchase Location and (2) Macronutrient Content...
January 30, 2018: Rapid Communications in Mass Spectrometry: RCM
Michail Dronov, Torsten C Schmidt, Jürgen Schram
RATIONALE: The concentration-gradient-method (CGM) was previously introduced as a precise and accurate method for isotope ratio determination by quadrupole-based inductively coupled plasma mass spectrometry (ICP-QMS). The investigation of its potential and advantages in the analysis of analytes with a poor signal-noise ratio (S/N) is important to establish routine isotope ratio analysis industrial applications on these widely used instruments. METHODS: CGM was applied on isotope measurements of Sr near its limit of quantification (LOQ) and of sulphur where there was a massively interfered 32 S isotope signal in gypsum samples of different origin, in order to demonstrate the advantages of CGM over the commonly used measurement and evaluation approach...
January 30, 2018: Rapid Communications in Mass Spectrometry: RCM
Stefanie Ickert, Jens Riedel, Sebastian Beck, Michael W Linscheid
RATIONALE: The most commonly used fragmentation methods in tandem MS are CID and HCD. While in CID, the preselected ions in the trap are resonantly (and m/z exclusively) excited, in HCD the entire m/z range experiences the dissociative acceleration. The different excitation is reflected in different fragment distributions. METHODS: As a test-bed for particularly pronounced fragmentation specificity, here MS/MS experiments on several 4-mer oligonucleotides were conducted employing both collision methods and the results were thoroughly compared...
January 25, 2018: Rapid Communications in Mass Spectrometry: RCM
Xiaoji Cao, Xue Cai, Weimin Mo
RATIONALE: The comparative study of higher-energy collisional dissociation(HCD) and collision-induced dissociation(CID) mechanisms for protonated cyclic indolyl α-amino esters in quadrupole/orbitrap(Q/Orbitrap) and quadrupole time-of-flight(QTOF) mass spectrometers, respectively, is helpful to study the structures and properties of biologically active indole derivatives using tandem mass spectrometry(MS/MS) technology. METHODS: HCD and CID experiments were carried out using electrospray ionization Q/Orbitrap MS and QTOFMS in positive ion mode, respectively...
January 25, 2018: Rapid Communications in Mass Spectrometry: RCM
Danielle Moncrieffe, Mark C Parkin, David Cowan
RATIONALE: Procollagen III amino-terminal propeptide (P-III-NP) is currently monitored in human doping control as a biomarker for growth hormone administration and also in clinical diagnostics using immunoassays. Drawbacks to this approach have been highlighted, and research is ongoing to develop a mass spectrometric method to complement these methods. However, a lack of traceable reference material, the presence of post translational modifications (PTMs), and small blood concentration implicate the development of targeted analytical methods for P-III-NP quantification...
January 23, 2018: Rapid Communications in Mass Spectrometry: RCM
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