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Rapid Communications in Mass Spectrometry: RCM

Makoto Suzuki, Shin Nishiumi, Takashi Kobayashi, Arata Sakai, Yosuke Iwata, Takato Uchikata, Yoshihiro Izumi, Takeshi Azuma, Takeshi Bamba, Masaru Yoshida
RATIONALE: The analytical stability and throughput of biomarker assays based on dried serum spots (DSS) are strongly dependent on the extraction process and determination method. In the present study, an on-line system based on supercritical fluid extraction-supercritical fluid chromatography coupled with tandem mass spectrometry (SFE-SFC/MS/MS) was established for analyzing the levels of disease biomarkers in DSS. METHODS: The chromatographic conditions were investigated using the ODS-EP, diol, and SIL-100A columns...
March 23, 2017: Rapid Communications in Mass Spectrometry: RCM
Marko Jovanović, Richard Tyldesley-Worster
RATIONALE: Qualitative data analysis of glycomic MS/MS data based on m/z values of product ions alone is widely used, and often sufficient for analysis of single analytes. However, most complex glycomic mixtures contain multiple isobaric oligosaccharides, in which case this approach is often limited. Here we show how ion intensity information can be used in order to enhance MS/MS data analysis, and extract both qualitative and semiquantitative information from complex glycomic MS/MS datasets...
March 15, 2017: Rapid Communications in Mass Spectrometry: RCM
Enrico Davoli, Antonio Bastone, Giancarlo Bianchi, Mario Salmona, Luisa Diomede
RATIONALE: Butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) are widely used to prevent oxidation and rancidity in foodstuffs, pharmaceutical preparations and cosmetic formulations. Although their safety has been thoroughly investigated, possible endocrine side-effects have been suggested. A useful method for the determination of BHA and BHT in foods is needed to estimate their daily intake through the diet. METHODS: We selected commercial chewing gum as a model of complex food matrix and developed a new method based on gas chromatography/mass spectrometry...
March 15, 2017: Rapid Communications in Mass Spectrometry: RCM
Alejandro Cohen, Neil W Ross, Paul M Smith, James P Fawcett
RATIONALE: 17β-Estradiol (E2), estrone (E1) and estriol (E3) are steroid hormones responsible for the regulation of the female reproductive system. Estradiol is planned to be used to feminize eels in aquaculture in order to improve their size and marketability. The residual levels of these hormones in fish tissue must be monitored to meet the requirements of food regulatory agencies. Few studies have studied these hormones on complex biological matrices such as fish tissue. METHODS: We developed a method to analyze E1, E2 and E3 in fish tissue using liquid chromatography in combination with differential ion mobility spectrometry (DMS) and tandem mass spectrometry (MS/MS)...
March 11, 2017: Rapid Communications in Mass Spectrometry: RCM
Christopher Ashwood, Jodie Louise Abrahams, Helena Nevalainen, Nicolle H Packer
RATIONALE: High protein production and secretion with eukaryotic glycosylation machinery make T. reesei RUT-C30 a suitable expression host for recombinant proteins. The N-glycosylation of secreted proteins of RUT-C30 is known to vary depending on culture nutrients but O-glycosylation has been less extensively studied. METHODS: O-glycans and glycopeptides from secreted proteins were separated by porous graphitised carbon and C-18 liquid chromatography, respectively...
March 9, 2017: Rapid Communications in Mass Spectrometry: RCM
Matthew T Briggs, Yin Ying Ho, Gurjeet Kaur, Martin K Oehler, Arun V Everest-Dass, Nicolle H Packer, Peter Hoffmann
RATIONALE: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) of the proteome of a tissue has been an established technique for the past decade. In the last few years, MALDI-MSI of the N-glycome has emerged as a novel MALDI-MSI technique. To assess the accuracy and clinical significance of the N-linked glycan spatial distribution, we have developed a method that utilises MALDI-MSI followed by liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) in order to assign glycan structures to the differentiating MALDI-MSI glycan masses released from the tissue glycoproteins...
March 8, 2017: Rapid Communications in Mass Spectrometry: RCM
Marcel Gaj, Stephan Kaufhold, Jeffrey J McDonnell
No abstract text is available yet for this article.
March 7, 2017: Rapid Communications in Mass Spectrometry: RCM
Lindsay L VanSomeren, Perry S Barboza, David D Gustine, M Syndonia Bret-Harte
RATIONALE: The use of stable isotopes for dietary estimates of wildlife assumes that there are consistent differences in isotopic ratios among diet items, and that the differences in these ratios between the diet item and the animal tissues (i.e., fractionation) are predictable. However, variation in isotopic ratios and fractionation of δ(13) C and δ(15) N values among locations, seasons, and forages are poorly described for arctic herbivores especially migratory species such as caribou (Rangifer tarandus)...
March 6, 2017: Rapid Communications in Mass Spectrometry: RCM
Eshwar Jagerdeo, Amanda Wriston
RATIONALE: This paper highlights the versatility of interfacing two ambient ionization techniques, Laser Diode Thermal Desorption (LDTD) and Atmospheric Solids Analysis probe (ASAP), to high resolution mass spectrometers and demonstrate the method's capability to rapidly generate high-quality data from multiple sample types with minimal, if any sample preparation. METHODS: For ASAP-MS analysis of solid and liquid samples, the material was transferred to a capillary surface before being introduced into the mass spectrometer...
March 6, 2017: Rapid Communications in Mass Spectrometry: RCM
Brian J Malys, Kevin G Owens
RATIONAL: Matrix assisted laser desorption ionization (MALDI) is widely used as the ionization method in high resolution chemical imaging studies that seek to visualize the distribution of analytes within sectioned biological tissues. This work extends the use of electrospray deposition (ESD) to apply matrix with an additional solvent spray to incorporate and homogenize analyte within the matrix overlayer. METHODS: Analytes and matrix are sequentially and independently applied by ESD to create a sample from which spectra are collected, mimicking a MALDI imaging mass spectrometry (IMS) experiment...
March 6, 2017: Rapid Communications in Mass Spectrometry: RCM
Derek A Smith, Alex L Sessions, Nathan Dalleska, Victoria J Orphan
RATIONALE: Dissolved sulfur species are of significant interest, both as important substrates for microbial activities and as key intermediaries in biogeochemical cycles. Species of intermediate oxidation state such as sulfite, thiosulfate, and thiols are of particular interest but are notoriously difficult to analyze, because of low concentrations and rapid oxidation during storage and analysis. METHODS: Dissolved sulfur species are reacted with monobromobimane which yields a fluorescent bimane derivative that is stable to oxidation...
March 1, 2017: Rapid Communications in Mass Spectrometry: RCM
Mohit Thummar, Prinesh N Patel, Arun L Petkar, Debasish Swain, R Srinivas, S Gananadhamu
RATIONALE: Saquinavir mesylate (SQM) is an antiviral drug used for the treatment of HIV infections. The identification and characterization of all degradation products is essential for achieving the quality in pharmaceutical product development and also for patient safety. METHODS: The drug was subjected to hydrolytic (HCl, NaOH and water), oxidative (H2 O2 ), photolytic (UV and florescence light) and thermal (dry heat) forced degradation conditions as per ICH guidelines...
February 24, 2017: Rapid Communications in Mass Spectrometry: RCM
S A Yates, N M Dempster, M F Murphy, S A Moore
RATIONALE: The lipid peroxidation product malondialdehyde forms M1 dG adducts with guanine bases in genomic DNA. The analysis of these adducts is important as a biomarker of lipid peroxidation, oxidative stress and inflammation which may be linked to disease risk or exposure to a range of chemicals. METHODS: Genomic DNA samples were subjected to acid hydrolysis to release the adducts in the base form (M1 G) alongside the other purines. A liquid chromatography-mass spectrometry method was optimised for the quantitation of the M1 G adducts in genomic DNA samples using product ion and multiple reaction mode scans...
February 23, 2017: Rapid Communications in Mass Spectrometry: RCM
Tetsuya Endo, Hideki Ogasawara, Moriaki Hayasaka, Yohei Hotta, Osamu Kimura, Klaus J Petzke
RATIONALE: As Hg accumulation in marine animals generally increases with increased trophic level (δ(15) N values) through the food web, predators accumulate higher levels of Hg. The main source of human Hg intake is the consumption of fish and other marine animals, and Hg concentration in scalp hair is the preferred marker for evaluating consumption of marine animals. Difference of δ(15) N values between trophic and source amino acids of human consumers could enable us to estimate the trophic level of consumer without knowing the bulk δ(15) N value of their prey...
February 22, 2017: Rapid Communications in Mass Spectrometry: RCM
Bernardo Valenti, Luisa Biondi, Luca Campidonico, Luana Bontempo, Giuseppe Luciano, Fortunato Di Paola, Venera Copani, Luca Ziller, Federica Camin
RATIONALE: No study has investigated the variations of stable isotope ratios (SIRs) of bioelements within a Protected Designations of Origin (PDO) cheese and few studies have focused on the dietary background of animals. For traceability purposes, it is important to know how and whether these issues affect SIRs in a PDO cheese. METHODS: Thirty-six Pecorino Siciliano cheese samples were collected in three east-Sicilian areas in seasons in which green herbage was present or absent in an animal's diet...
February 20, 2017: Rapid Communications in Mass Spectrometry: RCM
Bandar Alghanem, Frédéric Nikitin, Thomas Stricker, Eva Duchoslav, Jeremy Luban, Caterina Strambio-De-Castillia, Markus Muller, Frédérique Lisacek, Emmanuel Varesio, Gérard Hopfgartner
RATIONALE: In LC-MS peptide quantification, the optimization of multiple reaction monitoring (MRM) parameters is essential for sensitive detection. We have compared different approaches to build MRM assays, based either on flow injection analysis (FIA) of isotopically labelled peptides, or on the knowledge and the prediction of the best settings for MRM transitions and collision energies (CE). In this context, we introduce MRMOptimizer, an open source software tool that processes spectra and assists the user in selecting transitions in the FIA workflow...
February 15, 2017: Rapid Communications in Mass Spectrometry: RCM
Xinshu Guan, Bing Wang, Huixin Wang, Jinrong Liu, Ying Li, Xinhua Guo
RATIONALE: In certain conditions some amino acid (AA) residues undergo special reactions in the gas phase, generating characteristic neutral losses and product ions. Taking these special fragments into account and understanding the effect of AA residues on peptide cleavages will consummate database search algorithms and manual data interpretation in peptide sequencing by mass spectrometry (MS). In this study, the detail of the characteristic NH3 and CO losses of glutamine (Gln) residue located at the C-terminus of peptides is presented...
February 3, 2017: Rapid Communications in Mass Spectrometry: RCM
Erik A Hobbie
RATIONALE: Carbon isotope measurements on collagen and other tissues are used in modern and paleodiet studies, and relating these patterns to the dietary protein fraction (fdp ) and δ(13) C values of dietary protein and energy (lipids and carbohydrates) remains important for determining diets. METHODS: Carbon fluxes from dietary protein to tissues in feeding studies of mice, rats, and pigs were estimated to test how tissue δ(13) C values related to the δ(13) C values of dietary protein, dietary energy, fdp , or loge fdp ...
February 3, 2017: Rapid Communications in Mass Spectrometry: RCM
Julia A Bubis, Lev I Levitsky, Mark V Ivanov, Irina A Tarasova, Mikhail V Gorshkov
RATIONALE: Label-free quantification (LFQ) is a popular strategy for shotgun proteomics. A variety of LFQ algorithms have been developed recently. However, a comprehensive comparison of the most commonly used LFQ methods is still rare, in part due to a lack of clear metrics for their evaluation and an annotated and quantitatively well-characterized data set. METHODS: Five LFQ methods were compared: spectral counting based algorithms SIN , emPAI, and NSAF, and approaches relying on the extracted ion chromatogram (XIC) intensities, MaxLFQ and Quanti...
January 18, 2017: Rapid Communications in Mass Spectrometry: RCM
Clive Jones, David A Fike, Paula Peres
RATIONALE: IMS 7f-GEO isotope ratio applications increasingly involve analyses (e.g., S(-) or O(-) isotopes, coupled with primary ion currents < 30 pA) for which quasi-simultaneous arrival (QSA) could compromise precision and accuracy of data. QSA and associated correction have been widely investigated for the CAMECA NanoSIMS instruments, but not for the IMS series. METHODS: Sulfur and oxygen isotopic ratio experiments were performed using an electron multiplier (EM) detector, employing Cs(+) primary ion currents of 1, 2, 5 and 11...
January 17, 2017: Rapid Communications in Mass Spectrometry: RCM
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