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Functional Characterization of CYP6QE1 and CYP6FV21 in Resistance to λ-Cyhalothrin and Imidacloprid in Bradysia odoriphaga .
Journal of Agricultural and Food Chemistry 2024 January 31
Cytochrome P450 monooxygenases (P450s) belong to a family of metabolic enzymes that are involved in the detoxification of insecticides. In this study, our bioassay results showed that a field-collected population of Bradysia odoriphaga displayed a moderate resistance to λ-cyhalothrin and imidacloprid. Compared to susceptible population, CYP6QE1 and CYP6FV21 were significantly overexpressed in the field population. The expression of CYP6QE1 and CYP6FV21 was more abundant in the third and fourth larval stages, and CYP6QE1 and CYP6FV21 were most highly expressed in the midgut and Malpighian tubules. Exposure to λ-cyhalothrin and imidacloprid significantly increased the expression levels of CYP6QE1 and CYP6FV21 . Furthermore, the silencing of CYP6QE1 and CYP6FV21 significantly increased the susceptibility of B. odoriphaga larvae to λ-cyhalothrin and imidacloprid. The overexpression of CYP6QE1 and CYP6FV21 significantly enhanced the tolerance of transgenic Drosophila melanogaster lines to λ-cyhalothrin and imidacloprid. In addition, molecular docking revealed that these two P450 proteins have strong binding affinity toward λ-cyhalothrin and imidacloprid insecticides. Taken together, these results indicate that the overexpression of CYP6QE1 and CYP6FV21 is responsible for resistance to λ-cyhalothrin and imidacloprid in B. odoriphaga .
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