Antihyperglycemic activity of 14-deoxy, 11, 12-didehydro andrographolide on streptozotocin-nicotinamide induced type 2 diabetic rats.

BACKGROUND: Diabetic Mellitus is characterized by a lack or failure of insulin to bind to its target receptor or failure of the pancreas to yield insulin. This study evaluated the antihyperglycemic activity of 14-deoxy, 11, 12-didehydro andrographolide on streptozotocin-nicotinamide-induced type 2 diabetic rats. Diabetic conditions were induced by administering streptozotocin at a dosage of 45 mg/kg body weight and nicotinamide at a dosage of 110 mg/kg body weight through intraperitoneal injection.

MATERIALS AND METHODS: Diabetic-induced rats were treated with 14-deoxy, 11, 12-didehydro andrographolide concentrations between 10 and 500 mg/kg body weight. The blood glucose level and body weight of the rats were periodically examined. The pancreas was isolated and the histopathological staining was performed after making fine sections of the pancreas using a microtome. The influence of 14-deoxy, 11, 12-didehydro andrographolide on the expression level of various insulin signaling cascades was determined with q-PCR and western blotting.

RESULTS: The blood glucose level of the diabetic-induced rats was significantly (p < 0.05) higher when compared with the control group and resulted in a drop in the blood glucose level of the diabetic rats. Oral glucose level was also reduced in the treatment group and no significant reduction was noted in the untreated. The lipid profiling revealed that the atherogenic index and cholesterol ratio was increased in the diabetic group over the control group. Upregulation of the insulin cascades like IRTK and GLUT4 was observed by the q-PCR and upregulation of GLUT4 and IR-β was observed by the western blot analysis.

CONCLUSION: Overall, the finding indicates that 14-deoxy, 11, 12-didehydro andrographolide exhibited antihyperglycemic activity by modulating the expression of insulin cascades.