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Molecular Biology Reports

Muhammad Usman Akbar
Schiff base ligands are biologically active compounds in having antimicrobial, antiviral and antitumor activities etc. In this study, we have synthesized a Schiff base ligand namely 4-hydroxy-3-(2-hydroxy-3-methoxybenzylideneamino) benzoic acid, by reacting 3-amino-4-hydroxybenzoic acid and 2-hydroxy-3-methoxy benzaldehyde in the presence of acetic acid and refluxing it. The resulting base ligand was characterized on HPLC and used for radiolabelling with technetium-99m. The ligand 4-hydroxy-3-(2-hydroxy-3-methoxybenzylideneamino) benzoic acid was labelled with 99m Tc at pH 7, while reacting 230 µg of ligand with 15 mCi of 99m TcO- 4 for 10 min at room temperature...
October 16, 2018: Molecular Biology Reports
Leyla Saeidi, Hamid Ghaedi, Mirsaber Sadatamini, Rouhollah Vahabpour, Ali Rahimipour, Mehrnoosh Shanaki, Zahra Mansoori, Faranak Kazerouni
Long non-coding RNAs (LncRNAs) are non-coding RNAs. The potential roles of lncRNAs in type 2 diabetes mellitus (T2DM) are not well-known. In this study, we aim to assess the expression levels of LY86-AS1 and HCG27_201 in T2DM patients and a healthy control group. We obtained whole blood and serum samples from 100 T2DM and 100 non-diabetic subjects. Peripheral blood mononuclear cells (PBMCs) were extracted from whole blood samples using Ficoll. Total RNA was isolated from PBMCs obtained from patients with type 2 diabetes mellitus and healthy control individuals using TRIzol LS reagent (GeneAll Biotechnology Co...
October 16, 2018: Molecular Biology Reports
Farzad Zehsaz, Hamed Abbasi Soltani, Roya Hazrati, Negin Farhangi, Amir Monfaredan, Mehri Ghahramani
The purpose of the research was to examine if some genetic variations are associated with some endurance, power and speed performances (multi-stage 20-m shuttle run, standing broad jump, 20 m sprint test and Abalakov jump) in a group of 586 non-trained male adolescents (mean ± SD age: 13.20 ± 0.25 years). Polymorphisms in PPARa and PPARGC1A implicated in physical performance traits were analyzed. DNA was extracted and the samples were genotyped for PPARa and PPARGC1A polymorphisms by a PCR based method followed by gel electrophoresis...
October 15, 2018: Molecular Biology Reports
Alma Faviola Favela-Mendoza, Héctor Rangel-Villalobos, Ingrid Fricke-Galindo, Alberto Ortega-Vázquez, Gabriela Martínez-Cortés, Marisol López-López
The most widely studied polymorphisms of the ABCB1 gene are rs1128503 (c.1236C>T), rs2032582 (c.2677G>T/A), and rs1045642 (c.3435C>T). Although variation in ABCB1 allele frequencies among Mexican Mestizos (admixed) from different regions has been observed, Mexican Amerindians have been poorly studied. We aimed to describe the genetic variability of these three ABCB1 polymorphisms in a total sample of 273 Mexican volunteers that included Mestizos from the state of Yucatán, and Amerindians from seven populations (Tarahumara, Mayo, Huichol, Purépecha, Nahua, Tojolabal, and Maya)...
October 13, 2018: Molecular Biology Reports
Katarzyna Sułkowska-Ziaja, Agnieszka Szewczyk, Agnieszka Galanty, Joanna Gdula-Argasińska, Bożena Muszyńska
Fomitopsis betulina (Bull.) B.K. Cui, M.L. Han & Y.C. Dai has been used for medicinal purposes for over 5000 years. Numerous studies have confirmed the biological activity of compounds found in this species. The purpose of this study was a comparative analysis of selected groups of metabolites in the extracts from fruiting bodies and mycelial cultures. Phenolic acids (syringic, gallic, p-hydroxybenzoic, 3,4-dihydrophenylacetic), indole compounds (L-tryptophan, 5-hydroxy-L-tryptophan, 5-methyltryptamine), sterols (ergosterol, ergosterol peroxide, hexestrol, cholecalciferol), and triterpenes (betulinic acid, betulin) were determined quantitatively by high performance liquid chromatography with UV-Vis/DAD detection, while fatty acids were assessed with the gas chromatography method...
October 13, 2018: Molecular Biology Reports
Jésica Flores-Martín, Luciana Reyna, Mariano Cruz Del Puerto, María L Rojas, Graciela M Panzetta-Dutari, Susana Genti-Raimondi
StarD7 is a lipid binding protein involved in the delivery of phosphatidylcholine to the mitochondria whose promoter is activated by Wnt/β-catenin signaling. Although the majority of glucose enters glycolysis, ~ 2-5% of it can be metabolized via the hexosamine biosynthetic pathway (HBP). Considering that HBP has been implicated in the regulation of β-catenin we explored if changes in glucose levels modulate StarD7 expression by the HBP in trophoblast cells. We found an increase in StarD7 as well as in β-catenin expression following high-glucose (25 mM) treatment in JEG-3 cells; these effects were abolished in the presence of HBP inhibitors...
October 12, 2018: Molecular Biology Reports
Aarifa Nazmeen, Smarajit Maiti
N-ethyl-N-nitrosourea (ENU) is highly used in rodent models of tumerogenesis/carcinogenesis. Xenografting human-cancer tissues/cells with estradiol (E2) treatment is also used to generate rodent-models of gynaecological cancers. The altered metabolic-redox environment leading to establishment of pre-tumorigenesis condition and their mechanism are less studied. Here, female Wister rats were treated with these drugs at their pre-tumerogenic dosage (one group ENU single intra-peritoneal dose of 90 mg/kg b.w. and another group were implanted with human breast tumor (stage-IIIB) and fed with 2...
October 12, 2018: Molecular Biology Reports
Yaser Daanial Khan, Nouman Rasool, Waqar Hussain, Sher Afzal Khan, Kuo-Chen Chou
Protein phosphorylation is one of the most fundamental types of post-translational modifications and it plays a vital role in various cellular processes of eukaryotes. Among three types of phosphorylation i.e. serine, threonine and tyrosine phosphorylation, tyrosine phosphorylation is one of the most frequent and it is important for mediation of signal transduction in eukaryotic cells. Site-directed mutagenesis and mass spectrometry help in the experimental determination of cellular signalling networks, however, these techniques are costly, time taking and labour associated...
October 11, 2018: Molecular Biology Reports
Ilgin Kimiz-Gebologlu, Sultan Gulce-Iz, Cigir Biray-Avci
Nowadays, in cancer treatments, immunotherapy which can be classified as a cancer type specific therapy is more popular than non-specific therapy methods such as surgery, radiotherapy and chemotherapy. The main aim of immunotherapy is to enable patients' immune system to target cancer cells and destroy them. The mainly used treatment methods in cancer immunotherapy are cancer vaccines, adoptive cell therapy, cytokines and monoclonal antibodies. In this review, we discuss the immunotherapy approaches, especially monoclonal antibodies which are mostly used in cancer immunotherapy in clinical applications...
October 11, 2018: Molecular Biology Reports
Krishnamoorthy Vijayalakshmi, Chinnadurai Immanuel Selvaraj
Nature has gifted us with abundant plants possessing medicinal virtues which can cure several illnesses. Currently, diabetes mellitus is a severe threat to human well-being across the world due to the rapidly increasing incidence of diabetes. New effective bioactive drugs are in need, as plants do harbour and are proven to have potential antidiabetic activity than the present hypoglycemic medicines used in clinical therapy. In this study, in vitro cytotoxicity, glucose uptake, and anti-adipogenic activities of the plant extract (methanolic extract) of S...
October 11, 2018: Molecular Biology Reports
Laura Scherer Beier, Franciele Maboni Siqueira, Irene Silveira Schrank
Mycoplasmas belong to the Mollicutes class and possess low GC content and lack a cell wall, and also simplified metabolic pathways. Due to its reduced metabolic ability mycoplasmas are fastidious organisms growing with difficult under laboratory conditions. Its complex nutritional requirements render mycoplasmas to depend on external supplies of biosynthetic precursors. Aiming to develop and test defined media that could be used as a tool for Mycoplasma research, Mycoplasma hyopneumoniae and Mycoplasma hyorhinis were cultivated in a complex medium supplemented with serum (Friis broth) and in four different defined media (YUS, YUSm, CMRL and CMRL+, that was developed in the present study)...
October 11, 2018: Molecular Biology Reports
Miriam Trenado-Uribe, Mayra Silva-Miranda, José F Rivero-Cruz, Karol Rodríguez-Peña, Clara I Espitia-Pinzón, Romina Rodríguez-Sanoja, Sergio Sánchez
The search for new compounds effective against Mycobacterium tuberculosis is still a priority in medicine. The evaluation of microorganisms isolated from non-conventional locations offers an alternative to look for new compounds with antimicrobial activity. Endophytes have been successfully explored as source of bioactive compounds. In the present work we studied the nature and antimycobacterial activity of a compound produced by Streptomyces scabrisporus, an endophyte isolated from the medicinal plant Amphipterygium adstringens...
October 11, 2018: Molecular Biology Reports
Raman Kumar, Nidhi Gupta, Himani, Alpana Sharma
Shelterin complex and its associated molecules are imperative for proper functioning and maintenance of human telomeres. These molecules in association with human telomerase have been found altered in most cancers including multiple myeloma thereby proposed them as suitable therapeutic targets. Further, due to aggressive and recurring behavior of myeloma novel, efficacious and safe therapeutic agents for disease prevention are primary requirements for treatment of this disease. This maiden attempt evaluated the anti-proliferative properties of tanshinone I (TanI) alone or in combination with lenalidomide (Len) on myeloma cancer cell lines (RPMI8226 and U226)...
October 11, 2018: Molecular Biology Reports
Júlia Pereira Rodrigues, Ana Paula Ferranti Peti, Fernanda Salés Figueiró, Izadora de Souza Rocha, Vinicius Ricardo Acquaro Junior, Tamires Garcia Silva, Itamar Soares de Melo, Franklin Behlau, Luiz Alberto Beraldo Moraes
Citrus Canker disease is one of the most important disease in citrus production worldwide caused by gram-negative bacterial pathogen Xanthomonas citri subsp. citri, leading to great economic losses. Currently, a spray of copper-based bactericides is the primary measure for citrus canker management. However, these measures can lead to the contamination of soil by metal contamination, but also the development of copper-resistant Xanthomonas populations. Considering the need to discovery new alternatives to control the citrus canker disease, actinomycetes isolated from the Brazilian Caatinga biome and their crude extracts were tested against different strains of Xanthomonas citri subsp...
October 11, 2018: Molecular Biology Reports
Balraj Singh Gill, Richa Mehra, Navgeet, Sanjeev Kumar
Natural products are rich in several potent bioactive compounds, targeting complex network of proteins involved in various diseases. Vitex negundo (VN), commonly known as "chaste tree", is an ethnobotanically important plant with enormous medicinal properties. Different species of Vitex vary in chemical composition, thus producing different phytochemicals. Several bioactive compounds have been extracted from leaves, seeds, roots in form of volatile oils, flavonoids, lignans, iridoids, terpenes, and steroids...
October 11, 2018: Molecular Biology Reports
Venkatesh Kumaresan, Mukesh Pasupuleti, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Aziz Arshad, S M Nurul Amin, Fatimah Md Yusoff, Jesu Arockiaraj
Snakehead murrel, Channa striatus is an economically important aquatic species in Asia and are widely cultured and captured because of its nutritious and medicinal values. Their growth is predominantly affected by epizootic ulcerative syndrome (EUS) which is primarily caused by an oomycete fungus, Aphanomyces invadans. However, the molecular mechanism of immune response in murrel against this infection is still not clear. In this study, transcriptome technique was used to understand the molecular changes involved in C...
October 11, 2018: Molecular Biology Reports
Wasim Sajjad, Sajjad Ahmad, Iffat Aziz, Syed Sikander Azam, Fariha Hasan, Aamer Ali Shah
Streptomyces genus are filamentous Gram positive bacteria, of great intrest, producing biologically active compounds. Recent market and consumer curiosity in natural products have forced scientist and industry for the development of new products with therapeutic potential. This study focuses on evaluation of antioxidant and anticancerous properties of prodigiosin from radio-resistant Streptomyces sp. strain WMA-LM31. A molecular docking approach was adopted to understand theoretical binding mechanism and affinity for anticancer targets...
October 10, 2018: Molecular Biology Reports
Ming-Bing Xiao, Dan-Dan Jin, Yu-Jie Jiao, Wen-Kai Ni, Jin-Xia Liu, Li-Shuai Qu, Cui-Hua Lu, Run-Zhou Ni, Feng Jiang, Wei-Chang Chen
Psychological stress has been recognized as a well-documented risk factor associated with β2-adrenergic receptor (β2-AR) in the development of pancreatic cancer. Aldo-keto reductase 1 member B1 (AKR1B1) is a potential interacting partner of β2-AR, but the effect of their interaction on pancreatic cancer cells is not known at present. We found a positive correlation between AKR1B1 and β2-AR expression in pancreatic cancer tissue samples, and co-localization of these proteins in the human pancreatic cancer BXPC-3 cell line...
October 10, 2018: Molecular Biology Reports
Reihane Ebadi, Dor Mohammad Kordi-Tamandani, Kamran Ghaedi, Mohammad Hossein Nasr-Esfahani
Neural cells derived from embryonic stem cells (ESCs) have potential usefulness for the treatment of neurodegenerative disorders. Modulation of intrinsic growth factors expression such as neurotrophins and their respective receptors by these cells is necessary to obtain functional neural cells for transplantation. In present study, we compared neural differentiation potential of two different media, NB + 5%ES-FBS + N2B27 and Ko-DMEM + 5%ES-FBS for conversion of mESC derived neural progenitors (NPs) into mature neural cells with emphasis on effect of the these two media on neurotrophins and their respective receptors expression...
October 10, 2018: Molecular Biology Reports
Sonia Aloui, Wiem Zidi, Sana Ouali, Imen Guizani, Sameh Hadj-Taieb, Mohamed Sami Mourali, Moncef Feki, Monia Allal-Elasmi
Calcific mitral valve stenosis (MVS) is a common disease characterized by extensive remodeling of the extracellular matrix via matrix metalloproteinases (MMPs). The mechanism of calcification due to extensive matrix remodeling remains unclear. In this study, we investigated the relationship between MMP-3, tissue inhibitors of metalloproteinases (TIMPs) as well as pro-inflammatory cytokines and the phenomenon of calcification in MVS. 212 patients having rheumatic mitral stenosis (RMS) and 155 healthy control subjects were recruited in the Cardiology Department of La Rabta Hospital University...
October 9, 2018: Molecular Biology Reports
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