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Molecular Biology Reports

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https://www.readbyqxmd.com/read/28091786/a-new-look-at-the-drug-resistance-investigation-of-uropathogenic-e-coli-strains
#1
Wioletta Adamus-Białek, Łukasz Lechowicz, Anna B Kubiak-Szeligowska, Monika Wawszczak, Ewelina Kamińska, Magdalena Chrapek
Bacterial drug resistance and uropathogenic tract infections are among the most important issues of current medicine. Uropathogenic Escherichia coli strains are the primary factor of this issue. This article is the continuation of the previous study, where we used Kohonen relations to predict the direction of drug resistance. The characterized collection of uropathogenic E. coli strains was used for microbiological (the disc diffusion method for antimicrobial susceptibility testing), chemical (ATR/FT-IR) and mathematical (artificial neural networks, Ward's hierarchical clustering method, the analysis of distributions of inhibition zone diameters for antibiotics, Cohen's kappa measure of agreement) analysis...
January 13, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28070706/analysis-of-12-x-str-loci-in-the-population-of-south-croatia
#2
Gordan Mršić, Petar Ozretić, Josip Crnjac, Siniša Merkaš, Ivana Račić, Sara Rožić, Viktorija Sukser, Maja Popović, Marina Korolija
The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator(®) Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrelated donors. PCR products were analyzed by capillary electrophoresis. Population genetic and forensic parameters were calculated by the Arlequin and POPTREE2 software, and an on-line tool available at ChrX-STR.org...
January 9, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/27981446/comparative-capability-of-menstrual-blood-versus-bone-marrow-derived-stem-cells-in-neural-differentiation
#3
Fereshteh Azedi, Somaieh Kazemnejad, Amir Hassan Zarnani, Masoud Soleimani, Amir Shojaei, Shaghayegh Arasteh
In order to characterize the potency of menstrual blood stem cells (MenSCs) for future cell therapy of neurological disorders instead of bone marrow stem cells (BMSCs) as a well-known and conventional source of adult stem cells, we examined the in vitro differentiation potential of these stem cells into neural-like cells. The differentiation potential of MenSCs to neural cells in comparison with BMSCs was assessed under two step neural differentiation including conversion to neurosphere-like cells and final differentiation...
December 15, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27981445/patterns-of-sorafenib-and-tace-treatment-of-unresectable-hepatocellular-carcinoma-in-a-chinese-population-subgroup-analysis-of-the-gideon-study
#4
Fengyong Liu, Zhiqiang Meng, Guoliang Shao, Jianhua Wang, Zhijun Wang, Jijin Yang, Christina S M Yip, Dongfeng He
To analyze safety and efficacy of patterns of sorafenib and TACE therapy under real-life clinical practice conditions. A total of 338 Chinese patients with unresectable hepatocellular carcinoma (HCC) from the international database of the GIDEON non-interventional trial were included in this analysis. Endpoints were overall survival (OS), progression-free survival (PFS), time to progression (TTP) and safety. Two major patterns in the use of sorafenib observed in current Chinese clinical practice were: sorafenib administration subsequent to transarterial chemoembolization (TACE) treatment (n = 226, 66...
December 15, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27975165/screening-somatic-cell-nuclear-transfer-parameters-for-generation-of-transgenic-cloned-cattle-with-intragenomic-integration-of-additional-gene-copies-that-encode-bovine-adipocyte-type-fatty-acid-binding-protein-a-fabp
#5
Yong Guo, Hejuan Li, Ying Wang, Xingrong Yan, Xihui Sheng, Di Chang, Xiaolong Qi, Xiangguo Wang, Yunhai Liu, Junya Li, Hemin Ni
Somatic cell nuclear transfer (SCNT) is frequently used to produce transgenic cloned livestock, but it is still associated with low success rates. To our knowledge, we are the first to report successful production of transgenic cattle that overexpress bovine adipocyte-type fatty acid binding proteins (A-FABPs) with the aid of SCNT. Intragenomic integration of additional A-FABP gene copies has been found to be positively correlated with the intramuscular fat content in different farm livestock species. First, we optimized the cloning parameters to produce bovine embryos integrated with A-FABP by SCNT, such as applied voltage field strength and pulse duration for electrofusion, morphology and size of donor cells, and number of donor cells passages...
December 14, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27909922/production-of-biologically-active-recombinant-goose-fsh-in-a-single-chain-form-with-a-ctp-linker-sequence
#6
Hui Li, Huanxi Zhu, Qinming Qin, Mingming Lei, Zhendan Shi
FSH is a glycoprotein hormone secreted by the pituitary gland that is essential for gonadal development and reproductive function. In avian reproduction study, especially in avian reproduction hormone study, it is hindered by the lack of biologically active FSH. In order to overcome this shortcoming, we prepared recombinant goose FSH as a single chain molecule and tested its biological activities in the present study. Coding sequences for mature peptides of goose FSH α and β subunits were amplified from goose pituitary cDNA...
December 1, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27888418/suppression-of-mtor-signaling-pathway-promotes-bone-marrow-mesenchymal-stem-cells-differentiation-into-osteoblast-in-degenerative-scoliosis-in-vivo-and-in-vitro
#7
Yu Wang, Xiao-Dong Yi, Chun-De Li
To investigate the role of mTOR signaling pathway in bone marrow mesenchymal stem cells (BMSCs) differentiation into osteoblast in degenerative scoliosis (DS). The rat model of DS was established. Thirty-two Sprague-Dawley (SD) rats were selected and divided into the normal control group, the positive control group (normal rats injected with rapamycin), the negative control group (DS rats injected with PBS) and the experiment group (DS rats injected with rapamycin). H&E staining was performed to observe the osteogenesis of scoliosis...
November 25, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27878529/association-between-copy-number-variation-on-metabolic-phenotypes-and-hdl-c-levels-in-patients-with-polycystic-ovary-syndrome
#8
Birgit Knebel, Stefan Lehr, Onno E Janssen, Susanne Hahn, Sylvia Jacob, Ulrike Nitzgen, Dirk Müller-Wieland, Jorg Kotzka
Polygenic diseases with a broad phenotypic spectrum, such as polycystic ovary syndrome (PCOS), present a particular challenge in terms of identifying the underlying genetic mechanisms, nevertheless genetic variants have impact on the individual phenotype. We aimed to determine if next to genetic variations like SNPs further mechanisms might play a role in the pathogenesis of PCOS. We examined the effect of copy-number variations (CNVs) on metabolic phenotypes in PCOS. The intragenic rs1244979, rs2815752 in NEGR1 gene, and rs780094 in GCKR gene were genotyped and CNVs were determined by droplet digital polymerase chain reaction (ddPCR) in PCOS patients (n = 153) and controls without metabolic syndrome (n = 142)...
November 22, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27853974/isolation-and-characterization-of-lcsap-a-leymus-chinensis-gene-which-enhances-the-salinity-tolerance-of-saccharomyces-cerevisiae
#9
Jingying Liu, Xiangna Yang, Xizhe Yang, Mingyue Xu, Jie Liu, Mengmeng Xue, Pengda Ma
A number of members of the SAP ("stress-associated protein") gene family have been implicated in the plant stress response. Here, a SAP gene has been isolated using PCR RACE from the perennial grass Leymus chinensis, a species which has reputation for ecological adaptability. The 17.6 kDa LcSAP product comprised 161 residues, including both an A20 domain and an AN1 domain, a feature of type I SAPs. Using a semi-quantitative RT-PCR assay to profile its transcription, it was shown that LcSAP was more strongly transcribed in the leaf than in the root under control conditions...
November 16, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27844189/characterization-of-bcmf23a-and-bcmf23b-two-putative-pectin-methylesterase-genes-related-to-pollen-development-in-brassica-campestris-ssp-chinensis
#10
Sue Lin, Li Huang, Xiaolin Yu, Xingpeng Xiong, Xiaoyan Yue, Tingting Liu, Ying Liang, Meiling Lv, Jiashu Cao
Two homologous genes, Brassica campestris Male Fertility 23a (BcMF23a) and Brassica campestris Male Fertility 23b (BcMF23b), encoding putative pectin methylesterases (PMEs) were isolated from Brassica campestris ssp. chinensis (syn. Brassica rapa ssp. chinensis). These two genes sharing high sequence identity with each other were highly expressed in the fertile flower buds but silenced in the sterile ones of genic male sterile line system ('Bcajh97-01A/B'). Results of RT-PCR and in situ hybridization suggested that BcMF23a and BcMF23b were pollen-expressed genes, whose transcripts were first detected at the binucleate pollen and maintained throughout to the mature pollen grains...
November 14, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27812893/microarray-analysis-of-differential-gene-expression-profiles-in-blood-cells-of-naturally-blv-infected-and-uninfected-holstein-friesian-cows
#11
P Brym, S Kamiński
The aim of the present study was to examine gene expression changes in response to bovine leukemia virus (BLV) infection, in an effort to determine genes that take a part in molecular events leading to persistent lymphocytosis (PL), and to better define genes involved in host response to BLV infection. Using bovine 70-mer oligonucleotide spotted microarrays (BLOPlus) and qRT-PCR validation, we studied global gene expression profiles in blood cells in vivo of 12 naturally BLV-infected Polish Holstein cows, and 12 BLV non-infected controls of the same breed and reared in herds with high BLV seroprevalence...
November 3, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27752939/association-between-pnpla3-rs738409-lyplal1-rs12137855-ppp1r3b-rs4240624-gckr-rs780094-and-elevated-transaminase-levels-in-overweight-obese-mexican-adults
#12
Yvonne N Flores, Rafael Velázquez-Cruz, Paula Ramírez, Manuel Bañuelos, Zuo-Feng Zhang, Hal F Yee, Shen-Chih Chang, Samuel Canizales-Quinteros, Manuel Quiterio, Guillermo Cabrera-Alvarez, Nelly Patiño, Jorge Salmerón
There is scarce information about the link between specific single-nucleotide polymorphisms (SNPs) and risk of liver disease among Latinos, despite the disproportionate burden of disease among this population. Our aim was to investigate nine SNPs in or near the following genes: PNPLA3, LYPLAL1, PPP1R3B, GCKR, NCAN, IRS1, PPARG, and ADIPOR2 and examine their association with persistently elevated alanine aminotransferase (ALT) or aspartate aminotransferase (AST) levels in Mexican adults. Data and samples were collected from 741 participants in the Mexican Health Worker Cohort Study, in Cuernavaca, Mexico...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27730450/candidate-gene-studies-of-diabetic-retinopathy-in-human
#13
REVIEW
Petra Priščáková, Gabriel Minárik, Vanda Repiská
Diabetic retinopathy (DR) is a multifactorial disease with complex pathophysiology. It is the main cause of blindness among the people in productive age. The purpose of this literature review is to highlight recent achievements in the genetics of diabetic retinopathy with particular focus on candidate gene studies. We summarized most of the available published data about candidate genes for diabetic retinopathy with the goal to identify main genetic aspects. We conclude that genetic studies reported contradictory findings and no genetic variants meet criteria of a diagnostic marker, or significantly elucidate the root of DR development...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27655108/myd88-and-functionally-related-genes-are-associated-with-multiple-infections-in-a-model-population-of-kenyan-village-dogs
#14
Michaela Necesankova, Leona Vychodilova, Katerina Albrechtova, Lorna J Kennedy, Jan Hlavac, Kamil Sedlak, David Modry, Eva Janova, Mirko Vyskocil, Petr Horin
The purpose of this study was to seek associations between immunity-related molecular markers and endemic infections in a model population of African village dogs from Northern Kenya with no veterinary care and no selective breeding. A population of village dogs from Northern Kenya composed of three sub-populations from three different areas (84, 50 and 55 dogs) was studied. Canine distemper virus (CDV), Hepatozoon canis, Microfilariae (Acantocheilonema dracunculoides, Acantocheilonema reconditum) and Neospora caninum were the pathogens studied...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27649991/rna-sequencing-reveals-differential-thermal-regulation-mechanisms-between-sexes-of-glanville-fritillary-butterfly-in-the-tianshan-mountains-china
#15
Ying Lei, Yang Wang, Virpi Ahola, Shiqi Luo, Chongren Xu, Rongjiang Wang
The Glanville fritillary butterfly (Melitaea cinxia; Nymphalidae) has been extensively studied as a model species in metapopulation ecology. We investigated in the earlier studies that female butterflies exhibit higher thermal tolerance than males in the Tianshan Mountains of China. We aim to understand the molecular mechanism of differences of thermal responses between sexes. We used RNA-seq approach and performed de novo assembly of transcriptome to compare the gene expression patterns between two sexes after heat stress...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27649990/characterization-of-4-tagast-genes-during-spike-development-and-seed-germination-and-their-response-to-exogenous-phytohormones-in-common-wheat
#16
Yun Jeong Kim, Jae Yoon Kim, Jin Seok Yoon, Dae Yeon Kim, Min Jeong Hong, Yong Weon Seo
Gibberellic acid (GA) is involved in the regulation of plant growth and development. We defined GA-stimulated transcript (GAST) gene family and characterized its four members (TaGAST1, 2, 3, and 4) in wheat spikes. Triticum aestivum whole spikes were collected at ten developmental stages and dehulled spikelets were obtained at various days after flowering. Expression of TaGAST1, 2, 3, and 4 was analyzed using RT-PCR at inflorescence development stages, in different tissues, and after phytohormones application...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27640015/evaluating-anti-oxidant-potential-of-ganoderic-acid-a-in-stat-3-pathway-in-prostate-cancer
#17
Balraj Singh Gill, Sanjeev Kumar, Navgeet
Evaluating anti-oxidant potential of Ganoderic acid A in STAT 3 pathway in Prostate cancer. Molecular docking and ADMET activities of different isoforms of ganoderic acid on STAT 3 pathway were performed by Maestro 9.6 (Schrödinger Inc). The ganoderic acid A is best-docked among isoforms which analyses the expression level of antioxidant and STAT 3 pathway in PC-3 cells. The receptor-based molecular docking reveals the best binding interaction of SH2 domain of STAT3 and ganoderic acid A with GScore (-6.134), kcal/mol, Lipophilic EvdW (-1...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27640014/use-of-fourier-transform-infrared-spectroscopy-in-the-diagnosis-of-rheumatoid-arthritis-a-pilot-study
#18
Lukasz Lechowicz, Magdalena Chrapek, Jozef Gaweda, Mariusz Urbaniak, Iwona Konieczna
Rheumatoid arthritis is an autoimmune inflammatory disease leading to joint cartilage, bone degradation and limitation of mobility. Diagnosis of RA is difficult and complex. There are also no effective methods for clear discrimination between RA patients and non-RA individuals. In this work we use IR spectroscopy to differentiate RA patients and blood donors' sera. We found differences between investigated sera (RA and non-RA) in range of 3000-2800 and 1800-800 cm(-1) (W1-W5 regions). Based on mathematical analysis we developed a K-NN model characterized by 85 % of sensitivity and 100 % of specificity...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27633652/comparative-transcriptome-analysis-in-different-tissues-of-a-medicinal-herb-picrorhiza-kurroa-pinpoints-transcription-factors-regulating-picrosides-biosynthesis
#19
Ira Vashisht, Tarun Pal, Hemant Sood, Rajinder S Chauhan
Transcriptional regulation of picrosides biosynthesis, the iridoid glycosides of an endangered medicinal herb, Picrorhiza kurroa, is completely unknown. P. kurroa plants obtained from natural habitat accumulate higher picrosides than in-vitro cultured plants, which necessitates identification of transcription factors (TFs) regulating their differential biosynthesis. The current study investigates complete spectrum of different TF classes in P. kurroa transcriptomes and discerns their association with picrosides biosynthesis...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27631640/development-of-microsatellite-markers-using-next-generation-sequencing-for-the-columnar-cactus-echinopsis-chiloensis-cactaceae
#20
Carmen G Ossa, Isabel Larridon, Gioconda Peralta, Pieter Asselman, Fernanda Pérez
The aim of this study was to develop microsatellite markers as a tool to study population structure, genetic diversity and effective population size of Echinopsis chiloensis, an endemic cactus from arid and semiarid regions of Central Chile. We developed 12 polymorphic microsatellite markers for E. chiloensis using next-generation sequencing and tested them in 60 individuals from six sites, covering all the latitudinal range of this species. The number of alleles per locus ranged from 3 to 8, while the observed (Ho) and expected (He) heterozygosity ranged from 0...
December 2016: Molecular Biology Reports
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