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Molecular Biology Reports

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https://www.readbyqxmd.com/read/28929467/erratum-to-hfe-mrna-expression-is-responsive-to-intracellular-and-extracellular-iron-loading-short-communication
#1
Kosha J Mehta, Sebastien Farnaud, Vinood B Patel
The original article shows an error the co-author name: Sebastien Farnaud. The original article was corrected.
September 19, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28879539/synergistic-effect-of-two-%C3%AE-globin-gene-cluster-mutations-leading-to-the-hereditary-persistence-of-fetal-hemoglobin-hpfh-phenotype
#2
Priya Hariharan, Madhavi Sawant, Manju Gorivale, Ruma Manchanda, Roshan Colah, K Ghosh, Anita Nadkarni
Co-inheritance of gamma and beta globin gene mutations in a compound heterozygous state is rare but of clinical interest as it provides an important data on understanding the HbF expression. Hematological analysis was carried out (Sysmex KX-21). F-cells were enumerated using flow cytometry. Beta globin gene was analysed by CRDB technique and by DNA sequencing. Gamma globin promoter region was sequenced and expression studies were carried out using real time Taqman assay. We report a family, where two inherited defects of the β globin gene cluster segregate...
September 6, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28849415/segregation-of-a%C3%A2-novel-p-ser270tyr-maf-mutation-and-p-tyr56%C3%A2-crygd-variant-in-a-family-with-dominantly-inherited-congenital-cataracts
#3
Lubica Dudakova, Viktor Stranecky, Olga Ulmanova, Eva Hlavova, Marie Trková, Andrea L Vincent, Petra Liskova
A bilaterally blind woman, with a three generation family history of autosomal dominant congenital cataracts, variably associated with iris colobomata and microcornea, sought preconception genetic consultation. Whole-exome sequencing was performed in three affected family members, one unaffected first degree relative, and one spouse. The sequence variant c.168C>G; p.(Tyr56∗) in CRYGD, previously reported as pathogenic, and a novel mutation c.809C>A; p.(Ser270Tyr) in MAF, were identified in two affected family members; the grandmother, and half-brother of the proband...
August 28, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28840581/dishevelled-proteins-and-cyld-reciprocally-regulate-each-other-in-cml-cell-lines
#4
Ceyda Çalışkan, Melek Pehlivan, Zeynep Yüce, Ogun Sercan
Dishevelled (Dvl) proteins are activated by Wnt pathway stimulation and have crucial roles in the regulation of β-catenin destruction complex. CYLD is a tumor suppressor and a deubiquitination enzyme. CYLD negatively regulates the Wnt/β-catenin signaling pathway by deubiquitinating Dvl proteins. Loss of function and mutations of CYLD were linked to different types of solid tumors. Loss of function in CYLD is associated with Dvl hyper ubiquitination, resulting in the transmission of Wnt signaling to downstream effectors...
August 24, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28840433/the-amt1-family-genes-from-malus-robusta-display-differential-transcription-features-and-ammonium-transport-abilities
#5
Hui Li, Qing-Song Yang, Wei Liu, Jing Lin, You-Hong Chang
Ammonium is an important nitrogen sources for plant growth. In this study, we report on the gene characterization of the ammonium transporter AMT1 subfamily in the apple rootstock Malus robusta Rehd. Thirteen AMT genes were comprehensively evaluated from the apple genome (version 1.0). Then the gene features and expression patterns of five AMT1 members from M. robusta were analyzed. These genes fell into four clusters in the AMT phylogenetic tree: clade I (MrAMT1;1 and MrAMT1;3), clade II (MrAMT1;4), clade III (MrAMT1;2), and clade IV (MrAMT1;5)...
August 24, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28840425/hfe-mrna-expression-is-responsive-to-intracellular-and-extracellular-iron-loading-short-communication
#6
Kosha J Mehta, Sebastien Farnaud, Vinood B Patel
In liver hepatocytes, the HFE gene regulates cellular and systemic iron homeostasis by modulating cellular iron-uptake and producing the iron-hormone hepcidin in response to systemic iron elevation. However, the mechanism of iron-sensing in hepatocytes remain enigmatic. Therefore, to study the effect of iron on HFE and hepcidin (HAMP) expressions under distinct extracellular and intracellular iron-loading, we examined the effect of holotransferrin treatment (1, 2, 5 and 8 g/L for 6 h) on intracellular iron levels, and mRNA expressions of HFE and HAMP in wild-type HepG2 and previously characterized iron-loaded recombinant-TfR1 HepG2 cells...
August 24, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28808805/molecular-factors-regulating-e-cadherin-expression-in-urothelial-bladder-cancer-and-their-correlations-with-the-clinicopathological-features
#7
Samia Hussein, Hala Mosaad, Hayam E Rashed, Shimaa Ahmed, Ahmed Ragab, Eman I Ismail
This study aimed to assess the expression of S100A4, Twist and E-cadherin (mRNA and protein) in urothelial bladder cancer, investigate the correlation between them and evaluate their association with the clinicopathological features of the disease. The study included 54 patients diagnosed as urothelial bladder cancer of different stages and grades. The expression levels of S100A4, Twist and E-cadherin (mRNA and protein) in tissue samples were determined by quantitative RT-PCR and immunohistochemistry. The expression of S100A4 and Twist was significantly upregulated while E- cadherin was significantly downregulated in urothelial bladder cancer tissues compared to the adjacent surrounding normal bladder tissues at both mRNA and protein levels (p < 0...
August 14, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28795290/development-and-characterization-of-24-chloroplast-microsatellite-markers-for-two-species-of-eranthis-ranunculaceae
#8
Ami Oh, Byoung-Un Oh
Chloroplast microsatellites for two Korean endemic species, Eranthis byunsanensis and E. pungdoensis (Ranunculaceae), were isolated to address the questions of their distributional patterns and evolutionary relationships, using next-generation sequencing. Twenty-four polymorphic chloroplast microsatellite markers for these two species were developed, and then characterized in 65 individuals (55 individuals of E. byunsanensis and 10 individuals of E. pungdoensis). The number of alleles per locus ranged from 2 to 9; the average number of alleles across all the loci scored 4...
August 9, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28756560/in-silico-analysis-of-the-cyanobacterial-lectin-scytovirin-new-insights-into-binding-properties
#9
Andrei Santos Siqueira, Alex Ranieri Jerônimo Lima, Rafael Conceição de Souza, Alberdan Silva Santos, João Lídio da Silva Gonçalves Vianez Júnior, Evonnildo Costa Gonçalves
Scytovirin is a lectin isolated from the cyanobacterium Scytonema varium that has shown activity against HIV, SARS coronavirus and Zaire Ebola virus. Its 95 amino acids are divided into two structural domains (SD), the first spanning amino acids 1-48 (SD1) and the second 49-95 (SD2). Interestingly, the domains are nearly identical but differ in their affinities for carbohydrates. With the aim of enhancing understanding of the binding properties of scytovirin, we performed molecular dynamics (MD) simulations of scytovirin complexed with Man4...
July 29, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28755272/polymorphism-and-methylation-of-the-mc4r-gene-in-obese-and-non-obese-dogs
#10
Monika Mankowska, Joanna Nowacka-Woszuk, Aneta Graczyk, Paulina Ciazynska, Monika Stachowiak, Marek Switonski
The dog is considered to be a useful biomedical model for human diseases and disorders, including obesity. One of the numerous genes associated with human polygenic obesity is MC4R, encoding the melanocortin 4 receptor. The aim of our study was to analyze polymorphisms and methylation of the canine MC4R in relation to adiposity. Altogether 270 dogs representing four breeds predisposed to obesity: Labrador Retriever (n = 187), Golden Retriever (n = 38), Beagle (n = 28) and Cocker Spaniel (n = 17), were studied...
July 28, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28752270/combination-treatment-with-dendrosomal-nanocurcumin-and-doxorubicin-improves-anticancer-effects-on-breast-cancer-cells-through-modulating-cxcr4-nf-%C3%AE%C2%BAb-smo-regulatory-network
#11
Mohammad Amin Mahjoub, Babak Bakhshinejad, Majid Sadeghizadeh, Sadegh Babashah
Despite advantageous antitumor properties of doxorubicin, the considerable cytotoxicity of this chemotherapeutic agent has made it necessary to develop combination treatment strategies. The aim of the current study was to investigate the possible synergism between dendrosomal nanocurcumin (DNC) and doxorubicin in eliciting anticancer effects on MDA-MB-231 metastatic breast cancer cells. The expression levels of CXCL12/CXCR4 axis and Hedgehog pathway genes were evaluated in patient-derived breast carcinoma tissues by qRT-PCR...
July 27, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28748410/cytotoxic-effect-of-co-expression-of-human-hepatitis-a-virus-3c-protease-and-bifunctional-suicide-protein-fcu1-genes-in-a-bicistronic-vector
#12
Alexey Komissarov, Ilya Demidyuk, Dina Safina, Marina Roschina, Andrey Shubin, Nataliya Lunina, Maria Karaseva, Sergey Kostrov
Recent reports on various cancer models demonstrate a great potential of cytosine deaminase/5-fluorocytosine suicide system in cancer therapy. However, this approach has limited success and its application to patients has not reached the desirable clinical significance. Accordingly, the improvement of this suicide system is an actively developing trend in gene therapy. The purpose of this study was to explore the cytotoxic effect observed after co-expression of hepatitis A virus 3C protease (3C) and yeast cytosine deaminase/uracil phosphoribosyltransferase fusion protein (FCU1) in a bicistronic vector...
July 26, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28685248/genetic-risk-factors-for-myocardial-infarction-more-clearly-manifest-for-early-age-of-first-onset
#13
Boris V Titov, German J Osmak, Natalia A Matveeva, Nino G Kukava, Roman M Shakhnovich, Alexander V Favorov, Mikhail Ya Ruda, Olga O Favorova
Epidemiological genetics established that heritability in determining the risk of myocardial infarction (MI) is substantially greater when MI occurs early in life. However, the genetic architecture of early-onset and late-onset MI was not compared. We analyzed genotype frequencies of SNPs in/near 20 genes whose protein products are involved in the pathogenesis of atherosclerosis in two groups of Russian patients with MI: the first group included patients with age of first MI onset <60 years (N = 230) and the second group with onset ≥60 years (N = 174)...
July 6, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28676996/region-of-interest-methylation-analysis-a-comparison-of-msp-with-ms-hrm-and-direct-bsp
#14
Reem Akika, Zainab Awada, Nahed Mogharbil, Nathalie K Zgheib
The aim of this study was to compare and contrast three DNA methylation methods of a specific region of interest (ROI): methylation-specific PCR (MSP), methylation-sensitive high resolution melting (MS-HRM) and direct bisulfite sequencing (BSP). The methylation of a CpG area in the promoter region of Estrogen receptor alpha (ESR1) was evaluated by these three methods with samples and standards of different methylation percentages. MSP data were neither reproducible nor sensitive, and the assay was not specific due to non-specific binding of primers...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28664405/potential-association-between-itpkc-genetic-variations-and-hirschsprung-disease
#15
Jeong-Hyun Kim, Soo-Min Jung, Joong-Gon Shin, Hyun Sub Cheong, Jeong-Meen Seo, Dae-Yeon Kim, Jung-Tak Oh, Hyun-Young Kim, Kyuwhan Jung, Hyoung Doo Shin
Hirschsprung disease (HSCR) is a congenital and complex disorder characterized by intestinal obstruction due to the absence of enteric neurons along variable lengths of the hindgut. Our recent genome-wide association study (GWAS) has revealed regional associations with HSCR at several loci of inositol-trisphosphate 3-kinase C (ITPKC). For fine mapping, we additionally selected and genotyped a total of 12 single nucleotide polymorphisms (SNPs) of ITPKC in 187 HSCR patients and 283 unaffected controls, and performed a further combined imputation analysis based on genotype data from this second stage of fine mapping and our previous GWAS stage, totaling 902 subjects (187 HSCR cases and 715 controls)...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28601945/cadasil-mutant-notch3-r90c-decreases-the-viability-of-hs683-oligodendrocytes-via-apoptosis
#16
Mibo Tang, Changhe Shi, Bo Song, Jing Yang, Ting Yang, Chengyuan Mao, Yusheng Li, Xinjing Liu, Shuyu Zhang, Hui Wang, Haiyang Luo, Yuming Xu
Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is the most common hereditary cerebral small vessel disease caused by mutations in NOTCH3. Prevailing models suggest that demyelination occurs secondary to vascular pathology. However, in zebrafish, NOTCH3 is also expressed in mature oligodendrocytes. Thus, we hypothesized that in addition to vascular defects, mutant NOTCH3 may alter glial function in individuals with CADASIL. The aim of this study was to characterize the direct effects of a mutant NOTCH3 protein in HS683 oligodendrocytes...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28597412/association-of-a-604g-ghrelin-gene-polymorphism-and-serum-ghrelin-levels-with-the-risk-of-obesity-in-a-mexican-population
#17
Iris Monserrat Llamas-Covarrubias, Mara Anaís Llamas-Covarrubias, Erika Martinez-López, Eloy Alfonso Zepeda-Carrillo, Edgar Alfonso Rivera-León, Beatriz Palmeros-Sánchez, Juan Luis Alcalá-Zermeño, Sergio Sánchez-Enríquez
Obesity is a metabolic disorder that has a multifactorial etiology and affects millions of people worldwide. Ghrelin, a hormone coded by the GHRL gene, plays a role in human body composition and appetite. Single nucleotide polymorphisms (SNPs) of the GHRL gene have been associated with obesity and metabolic disorders. To evaluate the association of A-604G SNP of GHRL promoter region with serum ghrelin levels and the risk of obesity in a Mexican population. Two hundred and fifty individuals were enrolled and classified as obese or control subjects (CS) according to BMI...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28597411/comparative-proteomic-analysis-of-alfalfa-revealed-new-salt-and-drought-stress-related-factors-involved-in-seed-germination
#18
Qiaoli Ma, Junmei Kang, Ruicai Long, Tiejun Zhang, Junbo Xiong, Kun Zhang, Tenghua Wang, Qingchuan Yang, Yan Sun
Salinity and drought are two major environmental factors that limit the growth and yield of many forage crops in semi-arid and arid regions. Alfalfa (Medicago sativa L.) is one of the most important forage crops in many countries. We aim to investigate the molecular mechanisms of alfalfa in response to salt and drought stresses in this study. Physiological and proteomic analyses were applied to examine the Zhongmu NO.3 alfalfa seed germination stage with 200 mM NaCl and 180 g·L(-1) polyethylene glycol (PEG) treatments...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28551733/rt-lamp-assay-an-alternative-approach-for-profiling-of-bovine-heat-shock-protein-70-gene-in-pbmc-cultured-model
#19
Gyanendra Singh Sengar, Rajib Deb, T V Raja, Umesh Singh, Rajiv Kant, V Bhanuprakash, R R Alyethodi, Sushil Kumar, Preetam Verma, Soumendu Chakraborty, Rani Alex, Rani Singh
The purpose of this study is to develop a novel Reverse Transcriptase Loop-mediated isothermal amplification (RT-LAMP) based assay for in vitro profiling of heat shock protein 70 (Hsp70) in bovine peripheral blood mononuclear cell (PBMC) culture model utilizing the absorbance level of magnesium pyrophosphate-a by-product of LAMP reaction. A set of bovine Hsp70 specific RT-LAMP primers were designed to detect the differential absorbance level of magnesium pyrophosphate by-product which signifies the degree of Hsp70 amplification from cDNA of thermally induced cultured cells at different recovery periods...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28455686/flightless-i-homolog-regulates-glucocorticoid-receptor-mediated-transcription-via-direct-interaction-of-the-leucine-rich-repeat-domain
#20
Hong Lan Jin, Liu Yang, Kwang Won Jeong
Flightless-I homolog (FLII) is a member of the gelsolin family of proteins, and has been identified as a coactivator of estrogen receptor-mediated transcription. Here, we investigate the role of FLII in the glucocorticoid receptor (GR) signaling pathway. Reporter gene assay and real-time quantitative PCR in A549 were performed to investigate the function of FLII in the expression of GR target genes. Co-immunoprecipitation assay and in vitro binding assay were used to identify binding domain of FLII. Chromatin immunoprecipitation assay were carried out with FLII-depleted A549 cells to determine the role of FLII at GR binding sites...
April 28, 2017: Molecular Biology Reports
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