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Molecular Biology Reports

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https://www.readbyqxmd.com/read/28110431/a-report-on-identification-of-sequence-polymorphism-in-barcode-region-of-six-commercially-important-cymbopogon-species
#1
Ashok Kumar Bishoyi, Aarti Kavane, Anjali Sharma, K A Geetha
CYMBOPOGON: is an important member of grass family Poaceae, cultivated for essential oils which have greater medicinal and industrial value. Taxonomic identification of Cymbopogon species is determined mainly by morphological markers, odour of essential oils and concentration of bioactive compounds present in the oil matrices which are highly influenced by environment. Authenticated molecular marker based taxonomical identification is also lacking in the genus; hence effort was made to evaluate potential DNA barcode loci in six commercially important Cymbopogon species for their individual discrimination and authentication at the species level...
January 21, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28091786/a-new-look-at-the-drug-resistance-investigation-of-uropathogenic-e-coli-strains
#2
Wioletta Adamus-Białek, Łukasz Lechowicz, Anna B Kubiak-Szeligowska, Monika Wawszczak, Ewelina Kamińska, Magdalena Chrapek
Bacterial drug resistance and uropathogenic tract infections are among the most important issues of current medicine. Uropathogenic Escherichia coli strains are the primary factor of this issue. This article is the continuation of the previous study, where we used Kohonen relations to predict the direction of drug resistance. The characterized collection of uropathogenic E. coli strains was used for microbiological (the disc diffusion method for antimicrobial susceptibility testing), chemical (ATR/FT-IR) and mathematical (artificial neural networks, Ward's hierarchical clustering method, the analysis of distributions of inhibition zone diameters for antibiotics, Cohen's kappa measure of agreement) analysis...
January 13, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28070706/analysis-of-12-x-str-loci-in-the-population-of-south-croatia
#3
Gordan Mršić, Petar Ozretić, Josip Crnjac, Siniša Merkaš, Ivana Račić, Sara Rožić, Viktorija Sukser, Maja Popović, Marina Korolija
The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator(®) Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrelated donors. PCR products were analyzed by capillary electrophoresis. Population genetic and forensic parameters were calculated by the Arlequin and POPTREE2 software, and an on-line tool available at ChrX-STR.org...
January 9, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/27981446/comparative-capability-of-menstrual-blood-versus-bone-marrow-derived-stem-cells-in-neural-differentiation
#4
Fereshteh Azedi, Somaieh Kazemnejad, Amir Hassan Zarnani, Masoud Soleimani, Amir Shojaei, Shaghayegh Arasteh
In order to characterize the potency of menstrual blood stem cells (MenSCs) for future cell therapy of neurological disorders instead of bone marrow stem cells (BMSCs) as a well-known and conventional source of adult stem cells, we examined the in vitro differentiation potential of these stem cells into neural-like cells. The differentiation potential of MenSCs to neural cells in comparison with BMSCs was assessed under two step neural differentiation including conversion to neurosphere-like cells and final differentiation...
December 15, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27981445/patterns-of-sorafenib-and-tace-treatment-of-unresectable-hepatocellular-carcinoma-in-a-chinese-population-subgroup-analysis-of-the-gideon-study
#5
Fengyong Liu, Zhiqiang Meng, Guoliang Shao, Jianhua Wang, Zhijun Wang, Jijin Yang, Christina S M Yip, Dongfeng He
To analyze safety and efficacy of patterns of sorafenib and TACE therapy under real-life clinical practice conditions. A total of 338 Chinese patients with unresectable hepatocellular carcinoma (HCC) from the international database of the GIDEON non-interventional trial were included in this analysis. Endpoints were overall survival (OS), progression-free survival (PFS), time to progression (TTP) and safety. Two major patterns in the use of sorafenib observed in current Chinese clinical practice were: sorafenib administration subsequent to transarterial chemoembolization (TACE) treatment (n = 226, 66...
December 15, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27975165/screening-somatic-cell-nuclear-transfer-parameters-for-generation-of-transgenic-cloned-cattle-with-intragenomic-integration-of-additional-gene-copies-that-encode-bovine-adipocyte-type-fatty-acid-binding-protein-a-fabp
#6
Yong Guo, Hejuan Li, Ying Wang, Xingrong Yan, Xihui Sheng, Di Chang, Xiaolong Qi, Xiangguo Wang, Yunhai Liu, Junya Li, Hemin Ni
Somatic cell nuclear transfer (SCNT) is frequently used to produce transgenic cloned livestock, but it is still associated with low success rates. To our knowledge, we are the first to report successful production of transgenic cattle that overexpress bovine adipocyte-type fatty acid binding proteins (A-FABPs) with the aid of SCNT. Intragenomic integration of additional A-FABP gene copies has been found to be positively correlated with the intramuscular fat content in different farm livestock species. First, we optimized the cloning parameters to produce bovine embryos integrated with A-FABP by SCNT, such as applied voltage field strength and pulse duration for electrofusion, morphology and size of donor cells, and number of donor cells passages...
December 14, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27909922/production-of-biologically-active-recombinant-goose-fsh-in-a-single-chain-form-with-a-ctp-linker-sequence
#7
Hui Li, Huanxi Zhu, Qinming Qin, Mingming Lei, Zhendan Shi
FSH is a glycoprotein hormone secreted by the pituitary gland that is essential for gonadal development and reproductive function. In avian reproduction study, especially in avian reproduction hormone study, it is hindered by the lack of biologically active FSH. In order to overcome this shortcoming, we prepared recombinant goose FSH as a single chain molecule and tested its biological activities in the present study. Coding sequences for mature peptides of goose FSH α and β subunits were amplified from goose pituitary cDNA...
December 1, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27752939/association-between-pnpla3-rs738409-lyplal1-rs12137855-ppp1r3b-rs4240624-gckr-rs780094-and-elevated-transaminase-levels-in-overweight-obese-mexican-adults
#8
Yvonne N Flores, Rafael Velázquez-Cruz, Paula Ramírez, Manuel Bañuelos, Zuo-Feng Zhang, Hal F Yee, Shen-Chih Chang, Samuel Canizales-Quinteros, Manuel Quiterio, Guillermo Cabrera-Alvarez, Nelly Patiño, Jorge Salmerón
There is scarce information about the link between specific single-nucleotide polymorphisms (SNPs) and risk of liver disease among Latinos, despite the disproportionate burden of disease among this population. Our aim was to investigate nine SNPs in or near the following genes: PNPLA3, LYPLAL1, PPP1R3B, GCKR, NCAN, IRS1, PPARG, and ADIPOR2 and examine their association with persistently elevated alanine aminotransferase (ALT) or aspartate aminotransferase (AST) levels in Mexican adults. Data and samples were collected from 741 participants in the Mexican Health Worker Cohort Study, in Cuernavaca, Mexico...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27730450/candidate-gene-studies-of-diabetic-retinopathy-in-human
#9
REVIEW
Petra Priščáková, Gabriel Minárik, Vanda Repiská
Diabetic retinopathy (DR) is a multifactorial disease with complex pathophysiology. It is the main cause of blindness among the people in productive age. The purpose of this literature review is to highlight recent achievements in the genetics of diabetic retinopathy with particular focus on candidate gene studies. We summarized most of the available published data about candidate genes for diabetic retinopathy with the goal to identify main genetic aspects. We conclude that genetic studies reported contradictory findings and no genetic variants meet criteria of a diagnostic marker, or significantly elucidate the root of DR development...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27655108/myd88-and-functionally-related-genes-are-associated-with-multiple-infections-in-a-model-population-of-kenyan-village-dogs
#10
Michaela Necesankova, Leona Vychodilova, Katerina Albrechtova, Lorna J Kennedy, Jan Hlavac, Kamil Sedlak, David Modry, Eva Janova, Mirko Vyskocil, Petr Horin
The purpose of this study was to seek associations between immunity-related molecular markers and endemic infections in a model population of African village dogs from Northern Kenya with no veterinary care and no selective breeding. A population of village dogs from Northern Kenya composed of three sub-populations from three different areas (84, 50 and 55 dogs) was studied. Canine distemper virus (CDV), Hepatozoon canis, Microfilariae (Acantocheilonema dracunculoides, Acantocheilonema reconditum) and Neospora caninum were the pathogens studied...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27649991/rna-sequencing-reveals-differential-thermal-regulation-mechanisms-between-sexes-of-glanville-fritillary-butterfly-in-the-tianshan-mountains-china
#11
Ying Lei, Yang Wang, Virpi Ahola, Shiqi Luo, Chongren Xu, Rongjiang Wang
The Glanville fritillary butterfly (Melitaea cinxia; Nymphalidae) has been extensively studied as a model species in metapopulation ecology. We investigated in the earlier studies that female butterflies exhibit higher thermal tolerance than males in the Tianshan Mountains of China. We aim to understand the molecular mechanism of differences of thermal responses between sexes. We used RNA-seq approach and performed de novo assembly of transcriptome to compare the gene expression patterns between two sexes after heat stress...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27649990/characterization-of-4-tagast-genes-during-spike-development-and-seed-germination-and-their-response-to-exogenous-phytohormones-in-common-wheat
#12
Yun Jeong Kim, Jae Yoon Kim, Jin Seok Yoon, Dae Yeon Kim, Min Jeong Hong, Yong Weon Seo
Gibberellic acid (GA) is involved in the regulation of plant growth and development. We defined GA-stimulated transcript (GAST) gene family and characterized its four members (TaGAST1, 2, 3, and 4) in wheat spikes. Triticum aestivum whole spikes were collected at ten developmental stages and dehulled spikelets were obtained at various days after flowering. Expression of TaGAST1, 2, 3, and 4 was analyzed using RT-PCR at inflorescence development stages, in different tissues, and after phytohormones application...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27640015/evaluating-anti-oxidant-potential-of-ganoderic-acid-a-in-stat-3-pathway-in-prostate-cancer
#13
Balraj Singh Gill, Sanjeev Kumar, Navgeet
Evaluating anti-oxidant potential of Ganoderic acid A in STAT 3 pathway in Prostate cancer. Molecular docking and ADMET activities of different isoforms of ganoderic acid on STAT 3 pathway were performed by Maestro 9.6 (Schrödinger Inc). The ganoderic acid A is best-docked among isoforms which analyses the expression level of antioxidant and STAT 3 pathway in PC-3 cells. The receptor-based molecular docking reveals the best binding interaction of SH2 domain of STAT3 and ganoderic acid A with GScore (-6.134), kcal/mol, Lipophilic EvdW (-1...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27640014/use-of-fourier-transform-infrared-spectroscopy-in-the-diagnosis-of-rheumatoid-arthritis-a-pilot-study
#14
Lukasz Lechowicz, Magdalena Chrapek, Jozef Gaweda, Mariusz Urbaniak, Iwona Konieczna
Rheumatoid arthritis is an autoimmune inflammatory disease leading to joint cartilage, bone degradation and limitation of mobility. Diagnosis of RA is difficult and complex. There are also no effective methods for clear discrimination between RA patients and non-RA individuals. In this work we use IR spectroscopy to differentiate RA patients and blood donors' sera. We found differences between investigated sera (RA and non-RA) in range of 3000-2800 and 1800-800 cm(-1) (W1-W5 regions). Based on mathematical analysis we developed a K-NN model characterized by 85 % of sensitivity and 100 % of specificity...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27633652/comparative-transcriptome-analysis-in-different-tissues-of-a-medicinal-herb-picrorhiza-kurroa-pinpoints-transcription-factors-regulating-picrosides-biosynthesis
#15
COMPARATIVE STUDY
Ira Vashisht, Tarun Pal, Hemant Sood, Rajinder S Chauhan
Transcriptional regulation of picrosides biosynthesis, the iridoid glycosides of an endangered medicinal herb, Picrorhiza kurroa, is completely unknown. P. kurroa plants obtained from natural habitat accumulate higher picrosides than in-vitro cultured plants, which necessitates identification of transcription factors (TFs) regulating their differential biosynthesis. The current study investigates complete spectrum of different TF classes in P. kurroa transcriptomes and discerns their association with picrosides biosynthesis...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27631640/development-of-microsatellite-markers-using-next-generation-sequencing-for-the-columnar-cactus-echinopsis-chiloensis-cactaceae
#16
Carmen G Ossa, Isabel Larridon, Gioconda Peralta, Pieter Asselman, Fernanda Pérez
The aim of this study was to develop microsatellite markers as a tool to study population structure, genetic diversity and effective population size of Echinopsis chiloensis, an endemic cactus from arid and semiarid regions of Central Chile. We developed 12 polymorphic microsatellite markers for E. chiloensis using next-generation sequencing and tested them in 60 individuals from six sites, covering all the latitudinal range of this species. The number of alleles per locus ranged from 3 to 8, while the observed (Ho) and expected (He) heterozygosity ranged from 0...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27614750/association-of-p53-codon-72-polymorphism-and-survival-of-north-indian-lung-cancer-patients-treated-with-platinum-based-chemotherapy
#17
Ankita Kumari, Charu Bahl, Navneet Singh, Digambar Behera, Siddharth Sharma
p53 helps in maintaining genomic stability by undergoing cellular arrest, DNA repair or cellular apoptosis during DNA damage. So, as to find the association of p53Arg (72) Pro towards lung carcinogenesis and overall survival of North Indian lung cancer patients, single nucleotide polymorphic variant (rs1042522) was analyzed. 840 subjects including 420 cases and 420 controls were recruited and genotyped using PCR-RFLP technique for p53Arg (72) Pro polymorphic site. Association was analyzed using adjusted odds ratio along with its confidence intervals (95 % CI) and p value predicted from logistic regression whereas overall survival for lung cancer patients was obtained using Kaplan-Meir and Cox regression model for different parameters to obtain hazard ratio and survival time with statistical significance (log-rank p value)...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27585572/molecular-markers-a-potential-resource-for-ginger-genetic-diversity-studies
#18
REVIEW
Nor Asiah Ismail, M Y Rafii, T M M Mahmud, M M Hanafi, Gous Miah
Ginger is an economically important and valuable plant around the world. Ginger is used as a food, spice, condiment, medicine and ornament. There is available information on biochemical aspects of ginger, but few studies have been reported on its molecular aspects. The main objective of this review is to accumulate the available molecular marker information and its application in diverse ginger studies. This review article was prepared by combing material from published articles and our own research. Molecular markers allow the identification and characterization of plant genotypes through direct access to hereditary material...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27585571/a-preliminary-in-vivo-study-of-the-effects-of-opn-on-rat-liver-regeneration-induced-by-partial-hepatectomy
#19
Gaiping Wang, Congcong Zhao, Shasha Chen, Xiaofang Li, Ling Zhang, Cuifang Chang, Cunshuan Xu
Osteopontin (OPN) is a member of Th1 cytokine secreted by activated lymphocytes and macrophages. However, it deserves to be studied whether OPN could promote cell activation or proliferation, and then facilitate hepatic self-repair during liver regeneration (LR). This study is designed to further reveal the effects of OPN on LR in vivo. Firstly, quantitative reverse transcription-PCR (qRT-PCR) and western blot (WB) were utilized to validate the expression profile of endogenous OPN in rat regenerating livers after partial hepatectomy (PH)...
December 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27888418/suppression-of-mtor-signaling-pathway-promotes-bone-marrow-mesenchymal-stem-cells-differentiation-into-osteoblast-in-degenerative-scoliosis-in-vivo-and-in-vitro
#20
Yu Wang, Xiao-Dong Yi, Chun-De Li
To investigate the role of mTOR signaling pathway in bone marrow mesenchymal stem cells (BMSCs) differentiation into osteoblast in degenerative scoliosis (DS). The rat model of DS was established. Thirty-two Sprague-Dawley (SD) rats were selected and divided into the normal control group, the positive control group (normal rats injected with rapamycin), the negative control group (DS rats injected with PBS) and the experiment group (DS rats injected with rapamycin). H&E staining was performed to observe the osteogenesis of scoliosis...
November 25, 2016: Molecular Biology Reports
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