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Molecular Biology Reports

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https://www.readbyqxmd.com/read/28748410/cytotoxic-effect-of-co-expression-of-human-hepatitis-a-virus-3c-protease-and-bifunctional-suicide-protein-fcu1-genes-in-a-bicistronic-vector
#1
Alexey Komissarov, Ilya Demidyuk, Dina Safina, Marina Roschina, Andrey Shubin, Nataliya Lunina, Maria Karaseva, Sergey Kostrov
Recent reports on various cancer models demonstrate a great potential of cytosine deaminase/5-fluorocytosine suicide system in cancer therapy. However, this approach has limited success and its application to patients has not reached the desirable clinical significance. Accordingly, the improvement of this suicide system is an actively developing trend in gene therapy. The purpose of this study was to explore the cytotoxic effect observed after co-expression of hepatitis A virus 3C protease (3C) and yeast cytosine deaminase/uracil phosphoribosyltransferase fusion protein (FCU1) in a bicistronic vector...
July 26, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28685248/genetic-risk-factors-for-myocardial-infarction-more-clearly-manifest-for-early-age-of-first-onset
#2
Boris V Titov, German J Osmak, Natalia A Matveeva, Nino G Kukava, Roman M Shakhnovich, Alexander V Favorov, Mikhail Ya Ruda, Olga O Favorova
Epidemiological genetics established that heritability in determining the risk of myocardial infarction (MI) is substantially greater when MI occurs early in life. However, the genetic architecture of early-onset and late-onset MI was not compared. We analyzed genotype frequencies of SNPs in/near 20 genes whose protein products are involved in the pathogenesis of atherosclerosis in two groups of Russian patients with MI: the first group included patients with age of first MI onset <60 years (N = 230) and the second group with onset ≥60 years (N = 174)...
July 6, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28676996/region-of-interest-methylation-analysis-a-comparison-of-msp-with-ms-hrm-and-direct-bsp
#3
Reem Akika, Zainab Awada, Nahed Mogharbil, Nathalie K Zgheib
The aim of this study was to compare and contrast three DNA methylation methods of a specific region of interest (ROI): methylation-specific PCR (MSP), methylation-sensitive high resolution melting (MS-HRM) and direct bisulfite sequencing (BSP). The methylation of a CpG area in the promoter region of Estrogen receptor alpha (ESR1) was evaluated by these three methods with samples and standards of different methylation percentages. MSP data were neither reproducible nor sensitive, and the assay was not specific due to non-specific binding of primers...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28664405/potential-association-between-itpkc-genetic-variations-and-hirschsprung-disease
#4
Jeong-Hyun Kim, Soo-Min Jung, Joong-Gon Shin, Hyun Sub Cheong, Jeong-Meen Seo, Dae-Yeon Kim, Jung-Tak Oh, Hyun-Young Kim, Kyuwhan Jung, Hyoung Doo Shin
Hirschsprung disease (HSCR) is a congenital and complex disorder characterized by intestinal obstruction due to the absence of enteric neurons along variable lengths of the hindgut. Our recent genome-wide association study (GWAS) has revealed regional associations with HSCR at several loci of inositol-trisphosphate 3-kinase C (ITPKC). For fine mapping, we additionally selected and genotyped a total of 12 single nucleotide polymorphisms (SNPs) of ITPKC in 187 HSCR patients and 283 unaffected controls, and performed a further combined imputation analysis based on genotype data from this second stage of fine mapping and our previous GWAS stage, totaling 902 subjects (187 HSCR cases and 715 controls)...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28601945/cadasil-mutant-notch3-r90c-decreases-the-viability-of-hs683-oligodendrocytes-via-apoptosis
#5
Mibo Tang, Changhe Shi, Bo Song, Jing Yang, Ting Yang, Chengyuan Mao, Yusheng Li, Xinjing Liu, Shuyu Zhang, Hui Wang, Haiyang Luo, Yuming Xu
Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is the most common hereditary cerebral small vessel disease caused by mutations in NOTCH3. Prevailing models suggest that demyelination occurs secondary to vascular pathology. However, in zebrafish, NOTCH3 is also expressed in mature oligodendrocytes. Thus, we hypothesized that in addition to vascular defects, mutant NOTCH3 may alter glial function in individuals with CADASIL. The aim of this study was to characterize the direct effects of a mutant NOTCH3 protein in HS683 oligodendrocytes...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28597412/association-of-a-604g-ghrelin-gene-polymorphism-and-serum-ghrelin-levels-with-the-risk-of-obesity-in-a-mexican-population
#6
Iris Monserrat Llamas-Covarrubias, Mara Anaís Llamas-Covarrubias, Erika Martinez-López, Eloy Alfonso Zepeda-Carrillo, Edgar Alfonso Rivera-León, Beatriz Palmeros-Sánchez, Juan Luis Alcalá-Zermeño, Sergio Sánchez-Enríquez
Obesity is a metabolic disorder that has a multifactorial etiology and affects millions of people worldwide. Ghrelin, a hormone coded by the GHRL gene, plays a role in human body composition and appetite. Single nucleotide polymorphisms (SNPs) of the GHRL gene have been associated with obesity and metabolic disorders. To evaluate the association of A-604G SNP of GHRL promoter region with serum ghrelin levels and the risk of obesity in a Mexican population. Two hundred and fifty individuals were enrolled and classified as obese or control subjects (CS) according to BMI...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28597411/comparative-proteomic-analysis-of-alfalfa-revealed-new-salt-and-drought-stress-related-factors-involved-in-seed-germination
#7
Qiaoli Ma, Junmei Kang, Ruicai Long, Tiejun Zhang, Junbo Xiong, Kun Zhang, Tenghua Wang, Qingchuan Yang, Yan Sun
Salinity and drought are two major environmental factors that limit the growth and yield of many forage crops in semi-arid and arid regions. Alfalfa (Medicago sativa L.) is one of the most important forage crops in many countries. We aim to investigate the molecular mechanisms of alfalfa in response to salt and drought stresses in this study. Physiological and proteomic analyses were applied to examine the Zhongmu NO.3 alfalfa seed germination stage with 200 mM NaCl and 180 g·L(-1) polyethylene glycol (PEG) treatments...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28551733/rt-lamp-assay-an-alternative-approach-for-profiling-of-bovine-heat-shock-protein-70-gene-in-pbmc-cultured-model
#8
Gyanendra Singh Sengar, Rajib Deb, T V Raja, Umesh Singh, Rajiv Kant, V Bhanuprakash, R R Alyethodi, Sushil Kumar, Preetam Verma, Soumendu Chakraborty, Rani Alex, Rani Singh
The purpose of this study is to develop a novel Reverse Transcriptase Loop-mediated isothermal amplification (RT-LAMP) based assay for in vitro profiling of heat shock protein 70 (Hsp70) in bovine peripheral blood mononuclear cell (PBMC) culture model utilizing the absorbance level of magnesium pyrophosphate-a by-product of LAMP reaction. A set of bovine Hsp70 specific RT-LAMP primers were designed to detect the differential absorbance level of magnesium pyrophosphate by-product which signifies the degree of Hsp70 amplification from cDNA of thermally induced cultured cells at different recovery periods...
July 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28455686/flightless-i-homolog-regulates-glucocorticoid-receptor-mediated-transcription-via-direct-interaction-of-the-leucine-rich-repeat-domain
#9
Hong Lan Jin, Liu Yang, Kwang Won Jeong
Flightless-I homolog (FLII) is a member of the gelsolin family of proteins, and has been identified as a coactivator of estrogen receptor-mediated transcription. Here, we investigate the role of FLII in the glucocorticoid receptor (GR) signaling pathway. Reporter gene assay and real-time quantitative PCR in A549 were performed to investigate the function of FLII in the expression of GR target genes. Co-immunoprecipitation assay and in vitro binding assay were used to identify binding domain of FLII. Chromatin immunoprecipitation assay were carried out with FLII-depleted A549 cells to determine the role of FLII at GR binding sites...
April 28, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28451874/characterization-of-32-microsatellite-loci-for-the-pacific-red-snapper-lutjanus-peru-through-next-generation-sequencing
#10
David A Paz-García, Adrián Munguía-Vega, Tomas Plomozo-Lugo, Amy Hudson Weaver
We developed a set of hypervariable microsatellite markers for the Pacific red snapper (Lutjanus peru), an economically important marine fish for small-scale fisheries in the west coast of Mexico. We performed shotgun genome sequencing with the 454 XL titanium chemistry and used bioinformatic tools to search for perfect microsatellite loci. We selected 66 primer pairs that were synthesized and genotyped in an ABI PRISM 3730XL DNA sequencer in 32 individuals from the Gulf of California. We estimated levels of genetic diversity, deviations from linkage and Hardy-Weinberg equilibrium, estimated the frequency of null alleles and the probability of individual identity for the new markers...
April 27, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28432484/complete-mitochondrial-genome-sequences-of-korean-native-horse-from-jeju-island-uncovering-the-spatio-temporal-dynamics
#11
Sook Hee Yoon, Jaemin Kim, Donghyun Shin, Seoae Cho, Woori Kwak, Hak-Kyo Lee, Kyoung-Do Park, Heebal Kim
The Korean native horse (Jeju horse) is one of the most important animals in Korean historical, cultural, and economical viewpoints. In the early 1980s, the Jeju horse was close to extinction. The aim of this study is to explore the phylogenomics of Korean native horse focusing on spatio-temporal dynamics. We determined complete mitochondrial genome sequences for the first Korean native (n = 6) and additional Mongolian (n = 2) horses. Those sequences were analyzed together with 143 published ones using Bayesian coalescent approach as well as three different phylogenetic analysis methods, Bayesian inference, maximum likelihood, and neighbor-joining methods...
April 21, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28391434/perspectives-of-long-non-coding-rnas-in-cancer
#12
REVIEW
Arunagiri Kuha Deva Magendhra Rao, Thangarajan Rajkumar, Samson Mani
A recent advance in transcriptomics has spawned the 'Decade of non-coding RNAs' by potentiating the growing numbers of long non-coding RNA in cancer. LncRNA involvement in cancer denotes its significance beyond our perception as they participate in tumor suppression and promoting oncogenesis, which raises them as a mighty class of effectors or regulators. Aberrantly expressed lncRNAs interact with major protein and coding partners, which ultimately deregulate normal cellular processes and drive the cell towards malignant state...
April 8, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28389791/isolation-and-characterization-of-14-tetranucleotide-microsatellite-loci-for-the-cannonball-jellyfish-stomolophus-sp-by-next-generation-sequencing
#13
Leandro Nicolás Getino-Mamet, Tania Valdivia-Carrillo, Liza Gómez Daglio, Francisco Javier García-De León
The Cannonball jellyfish (Stomolophus sp.) is a species of jellyfish with high relevance in artisanal fishing. Studies of their populations do not extend beyond the morphological descriptions knowing that presents a great morphological variability. However, there are no genetic studies to determine the number of independent populations, so microsatellite markers become a suitable option. Since there are no species-specific microsatellite loci, in this paper, 14 new microsatellite loci are characterized. Microsatellite loci were isolated de novo through next generation sequencing, by two runs on Illumina MiSeq...
April 7, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28316001/associations-of-polymorphisms-in-the-candidate-genes-for-alzheimer-s-disease-bin1-clu-cr1-and-picalm-with-gestational-diabetes-and-impaired-glucose-tolerance
#14
Gabriela Vacínová, D Vejražková, P Lukášová, O Lischková, K Dvořáková, R Rusina, I Holmerová, H Vaňková, J Včelák, B Bendlová, M Vaňková
Alzheimer's disease (AD) is the most common type of dementia, with a prevalence that is rising every year. AD is associated with type 2 diabetes mellitus (T2DM) and insulin resistance, and is therefore sometimes called "type 3 diabetes mellitus". The aim of this study was to examine whether the variants of some candidate genes involved in the development of AD, namely BIN1 (rs744373), CLU (rs11136000), CR1 (rs3818361), and PICALM (rs3851179), are related to several disorders of glucose metabolism-gestational diabetes (GDM), T2DM and impaired glucose tolerance (IGT)...
March 18, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28281036/ghrelin-protected-neonatal-rat-cardiomyocyte-against-hypoxia-reoxygenation-injury-by-inhibiting-apoptosis-through-akt-mtor-signal
#15
Lifeng Wang, Yingjie Lu, Xian Liu, Xiaoyun Wang
Reducing reperfusion period myocardial cell damage is efficient to reduce myocardial ischemia-reperfusion injury. Ghrelin can increase myocardial contractility, improve heart failure caused by myocardial infarction. This study aimed to investigate the protective effect of Ghrelin on myocardial hypoxia/reoxygenation (H/R) injury of neonatal rat cardiomyocytes (NRCMs) and to explore the mechanisms. We isolated the NRCMs, established myocardial H/R model, blocked growth hormone secretagogue receptor (GHSR) by siRNA technique, examined cell activity by MTT and LDH assay, detected apoptosis by Hoechst 33258 staining and flow cytometry and determined the expression levels of apoptosis related proteins and signaling pathway proteins by western blot...
March 9, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28110431/a-report-on-identification-of-sequence-polymorphism-in-barcode-region-of-six-commercially-important-cymbopogon-species
#16
Ashok Kumar Bishoyi, Aarti Kavane, Anjali Sharma, K A Geetha
CYMBOPOGON: is an important member of grass family Poaceae, cultivated for essential oils which have greater medicinal and industrial value. Taxonomic identification of Cymbopogon species is determined mainly by morphological markers, odour of essential oils and concentration of bioactive compounds present in the oil matrices which are highly influenced by environment. Authenticated molecular marker based taxonomical identification is also lacking in the genus; hence effort was made to evaluate potential DNA barcode loci in six commercially important Cymbopogon species for their individual discrimination and authentication at the species level...
February 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28091786/a-new-look-at-the-drug-resistance-investigation-of-uropathogenic-e-coli-strains
#17
Wioletta Adamus-Białek, Łukasz Lechowicz, Anna B Kubiak-Szeligowska, Monika Wawszczak, Ewelina Kamińska, Magdalena Chrapek
Bacterial drug resistance and uropathogenic tract infections are among the most important issues of current medicine. Uropathogenic Escherichia coli strains are the primary factor of this issue. This article is the continuation of the previous study, where we used Kohonen relations to predict the direction of drug resistance. The characterized collection of uropathogenic E. coli strains was used for microbiological (the disc diffusion method for antimicrobial susceptibility testing), chemical (ATR/FT-IR) and mathematical (artificial neural networks, Ward's hierarchical clustering method, the analysis of distributions of inhibition zone diameters for antibiotics, Cohen's kappa measure of agreement) analysis...
February 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28070706/analysis-of-12-x-str-loci-in-the-population-of-south-croatia
#18
Gordan Mršić, Petar Ozretić, Josip Crnjac, Siniša Merkaš, Ivana Račić, Sara Rožić, Viktorija Sukser, Maja Popović, Marina Korolija
The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator(®) Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrelated donors. PCR products were analyzed by capillary electrophoresis. Population genetic and forensic parameters were calculated by the Arlequin and POPTREE2 software, and an on-line tool available at ChrX-STR.org...
February 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/27981446/comparative-capability-of-menstrual-blood-versus-bone-marrow-derived-stem-cells-in-neural-differentiation
#19
COMPARATIVE STUDY
Fereshteh Azedi, Somaieh Kazemnejad, Amir Hassan Zarnani, Masoud Soleimani, Amir Shojaei, Shaghayegh Arasteh
In order to characterize the potency of menstrual blood stem cells (MenSCs) for future cell therapy of neurological disorders instead of bone marrow stem cells (BMSCs) as a well-known and conventional source of adult stem cells, we examined the in vitro differentiation potential of these stem cells into neural-like cells. The differentiation potential of MenSCs to neural cells in comparison with BMSCs was assessed under two step neural differentiation including conversion to neurosphere-like cells and final differentiation...
February 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/27981445/patterns-of-sorafenib-and-tace-treatment-of-unresectable-hepatocellular-carcinoma-in-a-chinese-population-subgroup-analysis-of-the-gideon-study
#20
Fengyong Liu, Zhiqiang Meng, Guoliang Shao, Jianhua Wang, Zhijun Wang, Jijin Yang, Christina S M Yip, Dongfeng He
To analyze safety and efficacy of patterns of sorafenib and TACE therapy under real-life clinical practice conditions. A total of 338 Chinese patients with unresectable hepatocellular carcinoma (HCC) from the international database of the GIDEON non-interventional trial were included in this analysis. Endpoints were overall survival (OS), progression-free survival (PFS), time to progression (TTP) and safety. Two major patterns in the use of sorafenib observed in current Chinese clinical practice were: sorafenib administration subsequent to transarterial chemoembolization (TACE) treatment (n = 226, 66...
February 2017: Molecular Biology Reports
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