Effects of PAR2 Gene Knockout on Visceral Sensitivity, Stress Behaviors, and Colonic Electrical Activities in Irritable Bowel Syndrome.

BACKGROUND: Irritable bowel syndrome (IBS) could seriously affect the patient's health quality by its recurrence. There is a great medical and social need to explore the mechanisms and new treatment strategies of IBS.

OBJECTIVE: To explore the influence of protease-activated receptor 2 ( PAR2 ) gene knockout on IBS visceral sensitivity, stress behaviors, and colonic electrical activities.

METHODS: The PAR2 gene knockout and IBS model rats were generated and divided into PAR2 +/+ wild control (WC) group, PAR2 +/+ wild IBS model (WM) group, PAR2 -/- knockout control (KC) group and PAR2 -/- knockout IBS model (KM) group. The stress behaviors scores, minimum rectal fluid injection capacity threshold value of abdominal withdrawal reflex (AWR) = 3, and the colonic electrical activities indexes were recorded and the experimental results were analyzed statistically.

RESULTS: (1) PAR2 gene deletion and IBS models were successfully generated. (2) The scores of aggressive and exploratory behaviors in WM and KM groups were higher than WC and KC groups ( p < 0.05). The grooming behavior scores in WC and KC groups were higher than the WM and KM groups ( p < 0.05). WM group had the highest aggressive and exploratory behavior scores; WC group had the highest grooming behavior scores. (3) The minimum rectal fluid injection capacity threshold value of AWR = 3 in WM and KM groups were lower than WC and KC groups ( p < 0.05); WM group had the lowest value. (4) The maximum amplitude and wave frequency of colonic fast and slow waves in WM and WC groups were greater than in the KM and KC groups, respectively ( p < 0.05). The amplitude index and number of colonic contraction waves in WM and KM groups were greater than the WC and KC groups ( p < 0.05). Colonic electrical activity indexes were highest in the WM group.

CONCLUSIONS: The PAR2 gene deletion could have a beneficial effect on visceral sensitivity, stress behaviors and colonic electrical activities in rats with IBS.