Validation of a field-friendly faeces drying and storage method for quantifying faecal glucocorticoid metabolites in African elephants (Loxodonta africana) opens up new perspectives for conservationists.

Faecal glucocorticoid metabolites (fGCMs) are a relevant means of non-invasively assessing adrenocortical activity and thus, a key physiological stress response in wildlife populations. However, the widespread use of fGCMs as a stress-related biomarker in conservation biology is often hampered by the logistical challenge of storing collected faecal material frozen until it reaches the laboratory for analysis. Although alternative approaches to minimize potential alteration of fGCM composition post-defecation have been recently identified, there is to our knowledge, no satisfactory alternative method established for the preservation of elephant dung. In this study, we validated a field-friendly protocol for dehydrating African elephant faeces samples using a food dehydrator with desiccant and investigated the stability of fGCM concentrations in the dehydrated faeces when stored at ambient temperature. We collected 40 faecal samples from African elephants and compared fGCM concentrations of freeze-dried and dehydrated sample sub-sets. Samples dried in the field showed a slight but significant overall -6% reduction in fGCM concentration compared with frozen control samples. However, fGCM concentrations following field dehydration protocol match those of control samples with high accuracy, as evidenced by the low bias and strong coefficient of determination between the two approaches (R2  = 0.88). In addition, over nearly 2 months, storage time at ambient temperature of the dehydrated samples had no effect on the fGCM concentrations compared with those measured in the control samples (F-statistic = 1.82; P  = 0.18). Dehydrating the samples in the field thus provides an easy and cost-effective alternative to freezing, especially when working in remote areas with unstable electrical supply. Our results encourage the widespread use of fGCMs by conservationists as non-invasive means of steroid monitoring of African elephants in the current context of a general increase in wildlife welfare research. Future studies are needed to extend the use of this protocol to other species and to other steroid classes.