Dermal mesenchymal stem cells promoted adhesion and migration of endothelial cells by integrin in psoriasis.

The unusual dilatation of dermal capillaries and angiogenesis played important roles in psoriasis. Some genes and proteins of dermal mesenchymal stem cells (DMSCs) from psoriasis are abnormal and related to the function of endothelial cells (ECs). The present study was aimed to evaluate whether psoriatic DMSCs could affect adhesion and migration of ECs through neovascularization-related integrins in psoriasis. Human DMSCs, collected from psoriasis lesions and healthy skin respectively, were co-cultured with human umbilical vein endothelial cells (HUVECs). The expression levels of three integrins, i.e., αvβ3, αvβ5 and α5β1 in HUVECs were tested by quantitative real time polymerase chain reaction (qPCR) and western blot. The adhesion and migration of HUVECs were detected by adhesion assay and migration assay. The results showed that in psoriasis group, the expression of αVβ3 and α5β1 of HUVECs markedly increased 2.50-fold and 3.71-fold in mRNA levels, and significantly increased 1.63-fold and 1.92-fold in protein levels, comparing to healthy control group (all P<0.05). But β5 was not significantly different between the two groups (P>0.05). In addition, compared with control, psoriatic DMSCs promoted HUVECs adhesion by 1.62-fold and migration by 2.91-fold (all P<0.05). In conclusion, psoriatic DMSCs impact HUVECs adhesion and migration by up-regulating the expression of integrins αVβ3, α5β1. This article is protected by copyright. All rights reserved.