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Cell Biology International

M Martinez, I M U Rossetto, F S N Lizarte, L F Tirapelli, D P C Tirapelli, L G A Chuffa, V H A Cagnon, F E Martinez
Ethanol alters motricity, learning, cognition and cellular metabolism in the cerebellum. The combination of ethanol with caffeine by consuming "energy drinks" is becoming increasingly popular among young people. We analyzed the use of ethanol and caffeine on apoptosis in the cerebellum of UChB rats. The adult rats were divided into three groups (n = 14/group): UChB group: rats fed with 1:10 (v/v) ethanol ad libitum (free choice for water or ethanol) drinking from > 1.9 mL of ethanol/Kg body weight/day, Control group and UChB/caffeine group (free choice for water or ethanol + caffeine 300 mg/L)...
September 11, 2018: Cell Biology International
Yiwen Yang, Tao Wang, Degao Chen, Qizhao Ma, Yanjiang Zheng, Shiping Liao, Yufang Wang, Ji Zhang
Colorectal cancer (CRC) is the third most common type of cancer, and its incidence and mortality are markedly increasing worldwide. Oncogenic mutations of KRAS occur in up to 40% of CRC cases and pose a great challenge in the treatment of the disease. Quercetin is a dietary flavonoid that exerts anti-oxidant, anti-inflammatory and anti-cancer properties. The current study investigated the anti-proliferative effect of quercetin on CRC cells harboring mutant or wild-type KRAS. The effect of quercetin on cell viability was investigated by MTT and colony formation assays, and apoptosis was detected using flow cytometry by labeling cells with Annexin V-FITC...
September 11, 2018: Cell Biology International
Bo Yu, Yanan Zhao, Hongli Zhang, Di Xie, Wei Nie, Kaiyao Shi
MicroRNA-143-3p (miR-143-3p) is involved in the initiation of inflammatory response and the progression of cardiovascular diseases. Myocardial hypertrophy is a common symptom in numerous cardiovascular diseases. In the current study, we attempted to demonstrate the role of miR-143-3p in the development of myocardial hypertrophy by focusing on its association with inflammation. Myocardial hypertrophy was induced by transverse aortic constriction (TAC) method in vivo and by H2 O2 administration in vitro. The expression status of miR-143-3p and downstream effectors were detected in animal heart tissues and H9c2 cells...
September 11, 2018: Cell Biology International
Yanan Duan, Yinghui Dong, Rongguang Dang, Zhihui Hu, Ye Yang, Yueyun Hu, Jianxin Cheng
Ovarian cancer is one of the most common gyneacologic malignancies, with high morbidity and high mortality. Hsa-miR-122-5p (miR-122) has been reported with tumor-suppressing roles in various cancers. In this study, miR-122 was overexpressed in ovarian cancer cells, and phenotypic experiments demonstrated that miR-122 inhibited migration and invasion in SKOV3 and OVCAR3 cells. MiR-122 also suppressed epithelial mesenchymal transition (EMT), evidenced by expression changes of E-cadherin, vimentin, matrix metalloproteinase (MMP)2 and MMP14...
August 23, 2018: Cell Biology International
Muhammad Nihad As, Rucha Deshpande, Vaijayanti P Kale, Ramesh R Bhonde, Savita P Datar
Angiogenesis, the process of new blood vessel formation from pre-existing vessels, is essential for growth and development. Development of drugs that can accelerate or decelerate angiogenesis in the context of various diseases requires appropriate preclinical screening. As angiogenesis involves complex cellular and molecular processes, in vivo studies are superior to in vitro investigations. Conventional in vitro, in vivo, and ex ovo models of angiogenesis are time consuming and tedious, and require sophisticated infrastructure for embryo culture...
August 23, 2018: Cell Biology International
Hua Liu, Qixia Jiang, Zhiguo Ju, Shaofeng Guan, Ben He
Cardiovascular disease is a leading cause of death worldwide, requiring the development of new therapeutic strategies including stem cell therapy. Pentraxins (PTXs) are a superfamily of proteins highly involved in different myocardial disorders, and thus this study aimed to identify the modulation of long pentraxin 3 (PTX3) in the differentiation of mouse embryonic stem cells (mESCs) toward cardiomyocytes. Cell toxicity of PTX3 was detected by MTT and LDH assays in mESCs. Embryoid bodies (EBs) were differentiated using hanging drop method, and the beating was observed under microscope...
August 10, 2018: Cell Biology International
Ming Bai, Heping Yin, Jian Zhao, Yang Li, Yimin Wu
BACKGROUND: Human bone marrow mesenchymal stem cells (hBM-MSC) have the ability of differentiating into chondrocytes and osteoblasts. miR-182-5p promotes osteoclastogenesis and bone metastasis by up-regulating the expression of parathyroid hormone-like hormone (PTHLH). However, the function of miR-182-5p in chondrogenesis is still unknown. METHODS: Mimic or inhibitor of miR-182-5p were used to upregulate or knock-down miR-182-5p expression respectively. We analyzed chondrogenesis by Safranin O staining and Blyscan™ Sulfated Glycosaminoglycan Assay...
August 10, 2018: Cell Biology International
Rong Xue, Yingping Li, Xiaoli Li, Jingang Ma, Caiping An, Zhigang Ma
Kidney fibrosis is usually the final manifestation of a wide variety of renal diseases. Recent years, research reported that lncRNAs played important roles in a variety of human diseases. However, the role and underlying mechanisms of lncRNAs in kidney fibrosis were complicated and largely unclear. In our study, we constructed the cell model of renal fibrosis in HK2 cells using TGF-β1 and found that lncRNA MEG3 was down-regulated in TGF-β1-induced renal fibrosis. We then found that overexpressed MEG3 inhibited the TGF-β1-induced promotion of EMT, cell viability and proliferation...
August 10, 2018: Cell Biology International
Yuxin Xian, Liping Dong, Yong Jia, Yi Lin, Wenjuan Jiao, Yao Wang
miRNAs expression profiles in podocyte injuries have been reported in different models in vivo and in vitro. In the present study, miR-370 was elevated in high glucose-stimulated podocyte, and the post-transcriptional mechanism of miR-370 was investigated in high glucose-induced podocyte injuries. The gene and protein levels were assayed by RT-qPCR and Western blotting, respectively. Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining was used to evaluate the apoptosis in high glucose-stimulated podocyte...
August 10, 2018: Cell Biology International
Shounan Lu, Xingming Jiang, Zhilei Su, Zhankun Cui, Wen Fu, Sheng Tai
Long non-coding RNA (lncRNA) is one of the focuses and hotspots of biological research in recent years. At the same time, tumors have become the main disease that endangers human health. In recent years, a large number of researchers have explored the relationship between lncRNA and tumors. HOXA11-AS is one of these lncRNAs. The long non-coding RNA HOXA11 antisense RNA (HOXA11-AS) is a novel lncRNA recently discovered. It is found in a variety of tumors such as ovarian cancer, glioma, and gastric cancer (GC) and so on, and is defined as an oncogene...
August 10, 2018: Cell Biology International
Lina Wu, Zhen Chen, Yiqiao Xing
MicroRNAs (miRs) have been reported to participate in the initiation and progression of retinoblastoma (RB), as the most common primary intraocular malignancy during infancy and childhood worldwide. Recently, miR-506-3p has been reported to be associated with the development of RB, but the expression patterns and biological roles of miR-506-3p in RB have not been studied. In the present study, we found that miR-506-3p was significantly down-regulated in RB tissues and cell lines. Dual-luciferase reporter assay showed that miR-506-3p directly targeted mitosis Gene A (NIMA)-related kinase 6 (NEK6) in RB cells...
August 6, 2018: Cell Biology International
Maria Y Shubina, Yana R Musinova, Eugene V Sheval
Fibrillarin is an essential nucleolar protein that catalyzes the 2'-O-methylation of ribosomal RNAs. Recently, experimental data have begun to accumulate that suggest that fibrillarin can influence various cellular processes, development of pathological processes, and even aging. The exact mechanism by which fibrillarin can influence these processes has not been found, but some experimental data indicate that up- or downregulation of fibrillarin can modify the ribosome structure and, thus, causе an alteration in relative efficiency with which various mRNAs are translated...
August 6, 2018: Cell Biology International
Shiyong Wu, Wenhua Zhang, Shumin Ma, Bin Li, Chanjuan Xu, Ping Yi
Proliferation of the adult hepatocyte population represents a central feature of tissue regeneration after liver injury and resection. This process could be driven by a diverse range of mitogens, such as hepatocyte growth factor (HGF) and fibroblast growth factor (FGF). Among FGF family, FGF2 is closely related to wound repair and cell proliferation. FGF2 does function in the process of angiogenesis in regenerating liver, while fewer reports are concerned with the impact and underlying mechanism of FGF2 on liver cell proliferation...
August 6, 2018: Cell Biology International
Pelin Makaraci, Mariel Delgado Cruz, Hyoeun McDermott, Vy Nguyen, Chad Highfill, Kyoungtae Kim
Protein recycling is an important cellular process required for cell homeostasis. Results from prior studies have shown that Vps1, a dynamin homologue in yeast, is implicated in protein recycling from the endosome to the trans-Golgi Network (TGN). However, the function of Vps1 in relation to Ypt6, a master GTPase in the recycling pathway, remains unknown. The present study reveals that Vps1 physically interacts with Ypt6 if at least one of them is full-length. We found that overexpression of full-length Vps1, but not GTP hydrolysis-defective Vps1 mutants, is sufficient to rescue abnormal phenotypes of Snc1 distribution provoked by loss of Ypt6, and vice versa...
August 6, 2018: Cell Biology International
Weiwei Xi, Xuming Zhao, Meijun Wu, Wenjuan Jia, Hua Li
Although microRNA-155 (miR-155) is implicated in the pathogenesis of several fibrotic diseases, information regarding its functional role in renal fibrosis is limited. The current study aims to investigate the effects of miR-155 on renal fibrosis in unilateral ureteral occlusion (UUO) mice. MiR-155 level was significantly increased in renal tissues of UUO mice and TGF-β1-treated HK2 cells. Masson's trichrome staining showed that delivery of adeno-associated virus encoding miR-155 inhibitor led to a decrease in renal fibrosis induced by UUO...
August 6, 2018: Cell Biology International
Ali Rahiminejad, Rassoul Dinarvand, Behrooz Johari, Saeedeh Jafari Nodooshan, Ali Rashti, Elham Rismani, Parvin Mahdaviani, Zohreh Saltanatpour, Sajad Rahiminejad, Mozhgan Raigani, Masood Khosravani
Indirubin, an ingredient in traditional Chinese medicine, is considered as an anti-cancer agent. However, due to its hydrophobic nature, clinical efficiency has been limited. Drug delivery via nanotechnology techniques open new windows toward treatment of cancerous patients. Glioblastoma multiforme (GBM) is the most severe and common type of brain primary tumors. Of common problems in targeting therapies of glioblastoma is the availability of drug in tumoric tissues. In this study, Indirubin loaded solid lipid nanoparticles were prepared and their therapeutic potentials and antitumoric effects were assessed on GBM cell line (U87MG)...
August 6, 2018: Cell Biology International
Frank Gieseler, Corinna Plattfaut, Tabea Quecke, Annika Freund, Hendrik Ungefroren, Fanny Ender
Microvesicles (MVs) represent a subgroup of extracellular vesicles (EVs) emerging from various cells by blebbing of their outer membrane. Therefore, they share features such as membrane composition and antigenicity with their parental cells. Released by many immune and tumor cells, MVs act as intercellular messengers, account for horizontal gene transfer and can activate the coagulation system. With the aim to investigate their relevance for tumor cell biology, we characterized MVs released by human tumor cell lines of various origins in the absence or presence of TNF-α...
August 6, 2018: Cell Biology International
Jin Wang, Xue Liang, Tao Yu, Yu-Lin Xu, Li-Hua Xu, Xiao-Jing Zhang, Jie Ma, Yan-Ru Wang, Shuang-Li He
In human lung cancer, Tripartite motif 65 (TRIM65) is documented as an important regulator in carcinogenesis. Knockdown of TRIM65 prevents the tumorigenesis of lung cancer cells, while TRIM65 overexpression presents the opposite effect. However, the roles of TRIM65 in human lymphocyte malignancies have reported little. Herein, we found that Jurkat (T-lymphocyte) and Raji (B-lymphocyte) expressed TRIM65. We aimed to investigate whether TRIM65 was a potential oncogenic protein that regulated the tumorigenesis of Jurkat and Raji cells...
July 24, 2018: Cell Biology International
Bilu Xiang, Yang Liu, Wei Zhao, Hanchi Zhao, Haiyang Yu
Integrin αv β3 is a transmembrane integrin which can initiate osteoclasts' attachment on bone, leading to downward signaling pathways and subsequent bone resorption. Different calcium concentrations have reported to have influence on the activation of integrin αv β3 . To elucidate the regulatory mechanism of extracellular calcium concentrations on osteoclasts, a controlled micro flow plate (M04S) was utilized in the ONIX flow control system to observe osteoclasts' adhesion and migration in different calcium concentration medium...
July 18, 2018: Cell Biology International
Zedong Yan, Pan Wang, Junjie Wu, Xue Feng, Jing Cai, Mingming Zhai, Juan Li, Xiyu Liu, Maogang Jiang, Erping Luo, Da Jing
The effects of load-induced interstitial fluid shear stress (FSS) on instantaneous signaling response of osteocytes (e.g., calcium signaling) have been well documented. FSS can also initiate the release of many important messenger molecules of osteocytes (e.g., ATP and PGE2 ). However, the effects of FSS on cellular function and bone metabolism-modulating cytokine expression of osteocytes have not been fully identified (some inconsistent/conflicting results have been documented). Herein, osteocyte-like MLO-Y4 cells were stimulated with 1 Pa, 2-h FSS, and the effects of FSS on cellular morphology, cytoskeletal microstructure, biological activity, and gene and protein expression of important cytokines were investigated...
July 18, 2018: Cell Biology International
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