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Cell Biology International

Takaharu Taketomi, Tomohiro Onimura, Daigo Yoshiga, Daichi Muratsu, Terukazu Sanui, Takao Fukuda, Jingo Kusukawa, Seiji Nakamura
Fibroblast growth factor (FGF) and bone morphogenetic protein (BMP) play essential roles in bone formation and osteoblast activity through the extracellular signal regulated kinase 1/2 (ERK1/2) and Smad pathways. Sprouty family members are intracellular inhibitors of the FGF signaling pathway, and four orthologs of Sprouty have been identified in mammals. In vivo analyses have revealed that Sprouty2 is associated with bone formation. However, the mechanism by which the Sprouty family controls bone formation has not been clarified...
September 16, 2017: Cell Biology International
Xinmei Zhao, Chaolan Lv, Shenbo Chen, Fachao Zhi
The roles of tumor necrosis factor alpha (TNF-alpha) and its mediators in cellular processes related to intestinal diseases remain elusive. In this study, we aimed to determine the biological role of activated Cdc42-associated kinase 1 (ACK1) in TNF-alpha-mediated apoptosis and proliferation in Caco-2 cells. ACK1 expression was knocked down using ACK1-specific siRNAs, and ACK1 activity was disrupted using a small molecule ACK1 inhibitor. The Terminal deoxynucleotidyl transferase biotin-dUTP Nick End Labeling (TUNEL) and the BrdU incorporation assays were used to measure apoptosis and cell proliferation, respectively...
September 16, 2017: Cell Biology International
Paolella Gaetana, Lepretti Marilena, Martucciello Stefania, Nanayakkara Merlin, Auricchio Salvatore, Esposito Carla, Barone Maria Vittoria, Caputo Ivana
Alpha-gliadin peptide 31-43 is considered to be the main peptide responsible for the innate immune response in celiac disease patients. Recent evidence indicates that peptide 31-43 rapidly enters cells and interacts with the early endocytic vesicular compartment. However, the mechanism of its uptake is not completely understood. Our aim is to characterize, isolate and identify possible cell surface proteins involved in peptide 31-43 internalization by Caco-2 cells. In this study, we used a chemical cross-linker to block peptide 31-43 on cell surface proteins, and pulled-down peptide-proteins complexes using antibodies raised against peptide 31-43...
September 15, 2017: Cell Biology International
Yukari Kajiura, Yasufumi Nishikawa, Jung Hwan Lew, Jun-Ichi Kido, Toshihiko Nagata, Koji Naruishi
Periodontitis is associated with development of diabetes mellitus. Although lipopolysaccharide (LPS) of Porphyromonas gingivalis (Pg), a major pathogen of periodontitis, may lead the progression of diabetes complications, the precise mechanisms are unclear. We, therefore, investigated the effects of β-carotene on production of Pg LPS-induced inflammatory cytokines in human monocytes cultured high glucose (HG) condition. THP-1 cells were cultured under 5.5 mM or 25 mM glucose conditions, and cells were stimulated with Pg LPS...
September 14, 2017: Cell Biology International
Vivian Zague, Jonatas Bussador do Amaral, Paula Rezende Teixeira, Evandro Luis de Oliveira Niero, Camila Lauand, Glaucia Maria Machado-Santelli
Clinical data published in recent years has demonstrated positive effects of collagen hydrolysate (CH) on skin aging clinical signs. CH use as food supplement has a long history; however, few studies have addressed the underlying purpose of CH on the cellular and molecular biology of skin cells that could elucidate clinical improvement findings. Wide diversity of characteristics has been reported for dermal fibroblasts derived from different body sites and it is unknown whether collagen peptides could modulate differently cells from chronological aged and photoaged skin areas...
September 14, 2017: Cell Biology International
Mabel Buelna-Chontal, Martha Franco, Luz Hernández-Esquivel, Natalia Pavón, José S Rodríguez-Zavala, Francisco Correa, Ricardo Jasso, Gregorio Pichardo-Ramos, José Santamaría, Héctor González-Pacheco, Virgilia Soto, Jorge Luis Díaz-Ruíz, Edmundo Chávez
Heavy metal ions are known to produce harmful alterations on kidney function. Specifically, the accumulation of Hg(2+) in kidney tissue may induce renal failure. In this work the protective effect of CDP-choline against the deleterious effects induced by Hg(2+) on renal function were studied. CDP-choline administered ip at a dose of 125 mg/kg body weight prevented the damage induced by Hg(2+) administration at a dose of 3 mg/kg body weight. The findings indicate that CDP-choline guards mitochondria against Hg(2+) -toxicity by preserving their ability to retain matrix content, such as accumulated Ca(2+) ...
September 8, 2017: Cell Biology International
Xiaoyi Hu, Min Zhang, Jiyu Miao, Xiaofei Wang, Chen Huang
Studies have shown that miR-4317 is dysregulated in tumor, but the biologic role of miR-4317 in tumor development and progression remains unknown. The present study aimed to investigate the role of miR-4317 in human gastric cancer. Quantitative real-time PCR was used to quantify miR-4317 expression levels in clinical gastric cancer specimens and cell lines. MTT, colony formation and cell cycle assays were performed to identify the contributions of miR-4317 to cell proliferation in gastric cancer cell lines...
September 7, 2017: Cell Biology International
Yanlin Song, Lingqi Kong, Bin Sun, Lei Gao, Peng Chu, Anil Ahsan, Eskandar Qaed, Yuan Lin, Jinyong Peng, Xiaodong Ma, Jianbin Zhang, Shisheng Wang, Zeyao Tang
The signal transducers and activators of transcription 3 (STAT3) signaling pathway is a common feature in many solid tumors including non-small cell lung cancer, whereas current therapies usually fail to treat this disease in majority of cases. In the present study, we aimed to investigate the cytotoxic effect and the underlying mechanisms of SZC017, an oleanolic acid derivative, on human lung cancer cells. Cell viability was significantly decreased in SZC017-treated lung cancer cells. Mechanistically, SZC017 reduced A549 cell viability by activating both apoptosis and autophagy pathways...
September 7, 2017: Cell Biology International
Haile Li, Danping Liu, Chen Li, Shanjian Zhou, Dachuan Tian, Dawei Xiao, Huan Zhang, Feng Gao, Jianhua Huang
Mesenchymal stem cells (MSCs)-derived exosomes exhibit protective effects on damaged or diseased tissues. Hypoxia-inducible factor 1α (HIF-1α) plays a critical role in bone development. However, HIF-1α is easily biodegradable under normoxic conditions. The bone-marrow-derived mesenchymal stem cells (BMSCs) were transfected with adenovirus carrying triple point-mutations (amino acids 402, 564, and 803) in the HIF-1α coding sequence (CDS). The mutant HIF-1α can efficiently express functional proteins under normoxic conditions...
September 6, 2017: Cell Biology International
Danilo Ferrucci, Manoel F Biancardi, Umar Nishan, Rafaela Rosa-Ribeiro, Hernandes F Carvalho
In this commentary we propose a relationship between desquamation, initially described as the collective detachment and deletion of epithelial cell in the prostate gland after castration, and proliferative inflammatory atrophy (PIA) and stromal growth in benign prostate hyperplasia (BPH). First, in response to diverse stimuli, including inflammatory mediators, epithelial cells desquamate and leave a large surface of the luminal side of the basement membrane (BM) exposed. Basal cells are activated into intermediate-type cells, which change morphology to cover and remodel the exposed BM (simple atrophy) to a new physiological demand (such as in the hypoandrogen environment, simulated by surgical and/or chemical castration) and/or to support re-epithelialization (under normal androgen levels)...
September 6, 2017: Cell Biology International
Chun-Yang Wang, Zhao-Nan Sun, Ming-Xin Wang, Chao Zhang
Parkinson's disease (PD) is a progressive neurodegenerative disease, leading to tremor, rigidity, bradykinesia and gait impairment. Salidroside has been reported to exhibit antioxidative and neuroprotective properties in PD. However, the underlying neuroprotective mechanisms effects of salidroside are poorly understood. Recently, a growing body of evidences suggest that silent information regulator 1 (SIRT1) plays important roles in the pathophysiology of PD. Hence, the present study investigated the roles of SIRT1 in neuroprotective effect of salidroside against N-methyl-4-phenylpyridinium (MPP(+) )-induced SH-SY5Y cell injury...
August 29, 2017: Cell Biology International
Haibing Lan, Nan Wang, Yu Chen, Xiaojing Wang, Yuanqi Gong, Xiefei Qi, Yaling Luo, Fang Yao
Macrophage migration inhibitory factor (MIF) is an inflammatory mediator that contributes to asthmatic airway remodeling; however, little is known regarding the effects of MIF on airway smooth muscle cells (ASMCs). In the present study, we found that an enhanced expression of MIF promoted ASMC proliferation, increased the population of cells in the S/G2 phase, downregulated P21 expression, and upregulated cyclin D1, cyclin D3, and Cdk6 expression. In addition, the apoptosis of ASMCs was significantly decreased in response to MIF overexpression, compared with the negative control...
August 29, 2017: Cell Biology International
Xiaonan Shi, Chunlei Zheng, Ce Li, Kezuo Hou, Xiaoxun Wang, Zichang Yang, Chang Liu, Yunpeng Liu, Xiaofang Che, Xiujuan Qu
Dysregulation of histone acetylation plays an important role in tumor development. Histone acetylation regulates gene transcription and expression, which is reversibly regulated by histone acetyltransferase (HAT) and histone deacetylase (HDAC). As an HDAC inhibitor, 4-phenylbutyric acid (4-PBA) can increase histone acetylation levels by inhibiting HDAC activity. While 4-PBA inhibits proliferation of tumor cells in vitro, clinical trials have failed to show benefits of 4-PBA for refractory solid tumors. Here, we found that 4-PBA could enhance the migration capacity of gastric cancer cells...
August 29, 2017: Cell Biology International
Qi Tang, Chang Chen, Yan Zhang, Minjia Dai, Yichen Jiang, Hang Wang, Mei Yu, Wei Jing, Weidong Tian
The early stage of obesity is an important stage in the development of obesity. However there are few studies which explored the property or changes in obesity at early stage especially involving Wnt5a. The associated gene expression of Wnt5a on cell regeneration and the effect of Wnt5a on rat adipocyte-derived stem cell (rASC) proliferation and adipogenesis need additional study. Here, we investigated the changes in obesity at early stage and how Wnt5a regulates rASC regeneration, proliferation and adipogenesis...
August 29, 2017: Cell Biology International
Samanta Gasco, Amaya Rando, Pilar Zaragoza, Alberto García-Redondo, Ana Cristina Calvo, Rosarion Osta
Hematopoietic Stem and Progenitor Cells (HSPCs) are attractive targets in regenerative medicine, although the differences in their homeostatic maintenance between sexes along time are still under debate. We accurately monitored Hematopoietic Stem Cells (HSCs), Common Lymphoid Progenitors (CLPs) and Common Myeloid Progenitors (CMPs) frequencies by flow cytometry, by performing serial peripheral blood extractions from male and female B6SJL wild-type mice and found no significant differences. Only modest differences were found in the gene expression profile of Slamf1 and Gata2...
August 29, 2017: Cell Biology International
Wenzhi Li, Xiao Lin, Haoqing Yang, Yangyang Cao, Chen Zhang, Zhipeng Fan
Mesenchymal stem cells (MSCs) are a prospective cell source for tissue regeneration due to their self-renewal abilities and potential to differentiate into different cell lineages, but the molecular mechanisms of the directed differentiation and proliferation are still unknown. Recently, multiple studies have indicated the crucial role of HOX genes in MSC differentiation and proliferation. However, the role of HOXA5 in MSCs remains unknown. Here, we investigated HOXA5 function in stem cells from the apical papilla (SCAPs)...
August 22, 2017: Cell Biology International
Won-Seok Lee, Ji-Hyeon Jeong, Eun-Gyeong Lee, Yun Jung-Choi, Jin-Hee Kim, Hang-Rae Kim, Wan-Hee Yoo
Tacrolimus is an immunosuppressive drug that inhibits the release of inflammatory cytokines involved in rheumatoid arthritis development by blocking T cell activation. "Endoplasmic reticulum stress," an imbalance between protein folding load and capacity leading to the accumulation of unfolded proteins in the endoplasmic reticulum lumen, has been implicated in rheumatoid arthritis and other inflammatory and metabolic diseases. We aimed to investigate the effect of tacrolimus on endoplasmic reticulum stress-mediated osteoclastogenesis and inflammation and elucidate the underlying mechanisms...
August 22, 2017: Cell Biology International
Behrooz Johari, Jamil Zargan
Transcriptional master regulators like Sox2 and Oct4, which are expressed in various human tumors, have been shown to cause tumor growth promotion as well as epithelial dysplasia by means of interfering with progenitor cell differentiation. In order to investigate the potential of Sox2-Oct4 transcription factor decoy (TFD) strategy for differentiation therapy, mouse embryonic stem cells (mESCs) were used in this study as a model of cancer stem cells (CSCs). Sox2-Oct4 complex decoy ODNs (cd-ODNs) were designed according to their elements in the promoter region of Sox2 gene...
August 18, 2017: Cell Biology International
Dong Lin, Reza Izadpanah, Stephen E Braun, Eckhard Alt
BRCA1 plays a central role in DNA repair. Although N-terminal RING and C-terminal BRCT domains are studied, the functions of the central region of BRCA1 is poorly characterized. Here we report a structural and functional analysis of BRCA1 alleles and functional human BRCA1 in chicken B-lymphocyte cell line DT40. The combination of "Homologous Recombineering" and "RT-cassette" enables modifications of chicken BRCA1 gene in E.coli. Mutant BRCA1 knock-in DT40 cell lines were generated using BRCA1 mutation constructs by homologous recombination with a targeting efficiency of up to 100%...
August 18, 2017: Cell Biology International
Mizuyuki Ryu, Asuka Nogami, Tomoya Kitakaze, Naoki Harada, Yasushi A Suzuki, Ryoichi Yamaji
Dermal fibroblasts generate the extracellular matrix component elastin, which is synthesized as tropoelastin (TE) and play a critical role in maintaining skin elasticity. Lactoferrin (Lf), an 80-kDa iron-binding glycoprotein, has biological functions such as antibacterial, antiinflammatory, and anticancer activities. We previously reported that bovine Lf increases TE mRNA expression in human dermal fibroblasts. However, it remains unclear how Lf up-regulates TE expression. Here, we investigated molecular mechanisms underlying this effect...
August 18, 2017: Cell Biology International
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