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Cell Biology International

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https://www.readbyqxmd.com/read/28500745/advanced-oxidation-protein-products-induce-hepatocyte-epithelial-mesenchymal-transition-via-a-ros-dependent-tgf-%C3%AE-smad-signaling-pathway
#1
Shibo Sun, Fang Xie, Qifan Zhang, Zhonglin Cui, Xinsheng Cheng, Feng Zhong, Kun He, Jie Zhou
Epithelial-mesenchymal transition (EMT) occurs during the progression of liver fibrosis in response to chronic liver injury. However, the molecular mechanism underlying the regulation of hepatocyte EMT remains unclear. The aim of this study was to determine whether advanced oxidation protein products (AOPP) had an effect on hepatocyte EMT. The human L02 hepatocyte cell line and hepatocytes from normal Sprague-Dawley rats were challenged with AOPP treatment in both in vitro and in vivo studies. The expression of cell and molecular markers of EMT in L02 hepatocytes were studied using Western blotting, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays...
May 13, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28498509/molecular-interplays-in-hepatic-stellate-cells-apoptosis-senescence-and-phenotype-reversion-as-cellular-connections-that-modulates-liver-fibrosis
#2
REVIEW
Silva B O, Ramos L F, Moraes K C M
Liver fibrosis is a pathophysiological process correlated with intense repair and cicatrization mechanisms in injured liver, and over the past few years, the characterization of the fine-tuning of molecular interconnections that support the development of liver fibrosis has been investigated. In this cellular process, the hepatic stellate cells (HSCs) support the organ fibrogenesis. The HSCs are found in two distinct morpho-physiological states: quiescent and activated. In normal liver, most HSCs are found in quiescent state, presenting a considerable amount of lipid droplets in the cytoplasm, while in injured liver the activated phenotype of HSCs is a myofibroblast,that secretes extracellular matrix elements and contributes to the establishment of the fibrotic process...
May 12, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28493535/the-mongolian-gerbil-meriones-unguiculatus-as-a-model-for-inflammationpromoted-prostate-carcinogenesis
#3
Amado A Quintar, Bianca F Gonçalves, Sebastião R Taboga, Cristina A Maldonado
One of the recognized issues in prostate cancer research is the lack of animal models allowing the research of pathological, biochemical, and genetic factors in immunocompetent animals. Our research group has successfully employed the gerbil in several studisfor prostate diseases. In the present work, we aimed to analyze the effect of chronic bacterial inflammation on MNU-induced prostate carcinogenesis in gerbils Histopathological assessment of the prostatic complex revealed that treatment combinations with MNU plus testosterone or bacterial infection resulted in a promotion of prostate cancer, with bacterial inflammation being more effective in increasing premalignant and malignant tissular alterations than testosterone in the prostate...
May 11, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28493530/m2-macrophages-induce-emt-through-the-tgf-%C3%AE-smad2-signaling-pathway
#4
Liangying Zhu, Xiao Fu, Xiang Chen, Xiaodong Han, Ping Dong
IPF is characterized by fibroblast accumulation, collagen deposition and ECM remodeling, with myofibroblasts believed to be the effector cell type. Myofibroblasts develop due to EMT of lung alveolar epithelial cells, which can be induced by TGF-β. M2 macrophages, a macrophage subpopulation, secrete large amounts of TGF-β. To clarify the relationship between IPF, EMT, TGF-β and M2 macrophages, a bleomycin-induced pulmonary fibrosis mouse model was used. Seventeen days after mice were treated with bleomycin, the successful establishment of a pulmonary fibrosis model was confirmed by HE stain and Masson's trichrome stain...
May 11, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28493523/fibrillar-collagen-genes-are-not-co-ordinately-up-regulated-with-tgf-%C3%AE-1-expression-in-finasteride-treated-prostate
#5
Flávia Karina Delella, Fernanda Losi Alves de Almeida, Helga Caputo Nunes, Jaqueline Carvalho Rinaldi, Sérgio Luis Felisbino
Benign prostatic hyperplasia (BPH) is the most common cause of lower urinary tract symptoms (LUTS) in older men. In this regard, recent studies have attempted to define the relationships between prostatic fibrosis, LUTS, and increased expression of transforming growth factor β1 (TGF β1) in BHP. Therapeutic approaches for BPH such as 5-α-reductase inhibitors and alpha-adrenergic blocking agents increase TGF β1 expression in the prostatic tissue. Here, we investigated the effects of the 5-α-reductase inhibitor-finasteride-on rat ventral prostate tissue, especially with regard to the tissue distribution and gene expression of fibrillar collagens...
May 11, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28488774/fscn1-is-upregulated-by-snai2-and-promotes-epithelial-to-mesenchymal-transition-in-head-and-necksquamous-cell-carcinoma
#6
Lei Wang, YaoPeng Jia, ZengYu Jiang, Wei Gao, BinQuan Wang
In this study, we investigated whether there is any association between the expression of FSCN1 and SNAI2and the possible underlying mechanisms in head and neck squamous cell carcinoma (HNSC). In addition, we also investigated whether FSCN1 modulates epithelial to mesenchymal transition (EMT) in HNSC cells. Microarray data of dysregulated genes in HNSC was searched in GEO datasets. The association between FSCN1 expression and the 5-year/10-year overall survival (OS), as well as the correlation between the expression of FSCN1 and SOX2, MYBL2, SNAI2, STAT1 and SOX4 were analyzed based on data in TCGA HNSC cohort (TCGA-HNSC)...
May 10, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28477422/prostate-cancer-molecular-profiling-the-achille-s-heel-for-the-implementation-of-precision-medicine
#7
REVIEW
Eliane Gouvêa de Oliveira Barros, Pedro Nicolau-Neto, Nathalia Meireles Da Costa, Luís Felipe Ribeiro Pinto, Antonio Palumbo Jr, Luiz Eurico Nasciutti
Cancer has been mainly treated by traditional therapeutic approaches which do not consider the human genetic diversity and present limitations, probably as a consequence of a poor knowledge of both patient's genetic background and tumor biology. Due to genome project conclusion and large scale gene analyses emergence, the therapeutic management of several prevalent and aggressive tumors has dramatically improved and represents the closest examples of a precision medicine intervention in this field. Nonetheless, prostate cancer (PCa) remains as a challenge to personalized medicine implementation, probably due to its notorious heterogeneous molecular profile...
May 6, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28464448/gene-expression-changes-of-thermo-sensitive-transient-receptor-potential-channels-in-obese-mice
#8
Wuping Sun, Chen Lib, Yonghong Zhang, Changyu Jiang, Mingzhu Zhai, Qian Zhou, Lizu Xiao, Qiwen Deng
Adipose tissues play key roles in energy homeostasis. Brown adipocytes and beige adipocytes in white adipose tissue (WAT) sharethe similar characters of thermogenesis, both of them could be potential targets for obesity management. Several thermo-sensitive transient receptor potential channels (thermoTRPs) are shown to be involved in adipocyte biology. However, the expression pattern of thermoTRPs in adipose tissues from obese mice is still unknown. The mRNA expression of thermoTRPs in subcutaneous WAT (sWAT) and interscapular brown adipose tissue (iBAT) from lean and obese mice were measured using Reverse Transcriptase-quantitative PCRs (RT-qPCR)...
May 2, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28464412/heart-specific-expression-of-laminopathic-mutations-in-transgenic-zebrafish
#9
Ajay D Verma, Veena K Parnaik
Lamins are key determinants of nuclear organization and function in the metazoan nucleus. Mutations in human lamin A cause a spectrum of genetic diseases that affect cardiac muscle and skeletal muscle as well as other tissues. A few laminopathies have been modeled using the mouse. As zebrafish is a well established model for the study of cardiac development and disease, we have investigated the effects of heart-specific lamin A mutations in transgenic zebrafish. We have developed transgenic lines of zebrafish expressing conserved lamin A mutations that cause cardiac dysfunction in humans...
May 2, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28462515/tab3-defect-induces-augmented-cardioprotection-loss-from-ischemic-injury
#10
Yang Sheng, Changfu Xu, Wenping Zeng
The transforming growth factor β-activated kinase-binding protein 3 (TAB3) plays a crucial role in modulating cell apoptosis and proliferation in many diseases, including hepatocellular carcinoma, lung cancer and intracerebral hemorrhage. However, the functional role of TAB3 in the heart is not well reported. In our study, we first investigated the role of TAB3 in ischaemia heart diseases. For in vitro studies, cardiomyocytes were isolated from both TAB3 knockout (KO) and WT mice, and the apoptosis ratios were tested after a 48-h ischaemic stimulation...
April 29, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28462511/altered-expression-of-enos-prostacyclin-synthase-prostaglandin-g-h-synthase-and-thromboxane-synthase-in-porcine-aortic-endothelial-cells-after-exposure-to-human-serum-relevance-to-xenotransplantation
#11
Pengfei Chen, Hanchao Gao, Ying Lu, Huirong Nie, Zhaoming Liu, Yu Zhao, Nana Fan, Qingjian Zou, Yifan Dai, Aifa Tang, Hidetaka Hara, Zhiming Cai, David K C Cooper, Liangxue Lai, Lisha Mou
Under normal conditions, the activity of platelets is stringently and precisely balanced between activation and quiescent state. This guarantees rapid hemostasis and avoids uncontrolled thrombosis. However, excessive platelet activation and resulting thrombotic microangiopathy are frequently observed in pig-to-primate xenotransplantation models. Endothelium-derived inhibitory mechanisms play an important role in regulation of platelet activation. These mainly include nitric oxide (NO), prostacyclin PGI2 and adenosine, which are synthesized by endothelial nitric oxide synthases (eNOS), prostacyclin synthase, and CD39/CD73, respectively...
April 29, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28462510/runx2-alleviates-high-glucose-suppressed-osteogenic-differentiation-via-pi3k-akt-gsk3%C3%AE-%C3%AE-catenin-pathway
#12
Yang Chen, Yun Hu, Lan Yang, Jie Zhou, Yuying Tang, Leilei Zheng, Pu Qin
Hyperglycemia is one of the most important pathogenesis of diabetic osteopathy. Several lines of studies indicate Runx2 plays a critical role in the process of osteogenic differentiation. However, little studies have analysed the effect of Runx2 on osteoblast differentiation of rat bone mesenchymal stem cells (rBMSCs) in high glucose condition. In this study, the effect of Runx2 on osteoblast differentiation in high glucose condition was evaluated by the expression of osteogenesis-related maker including Runx2, ALP, OC and OPN, as well as ALP staining, ALP activity and Alizarin red S staining...
April 29, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28460425/the-schlafen-family-complex-roles-in-different-cell-types-and-virus-replication
#13
REVIEW
Furao Liu, Pingting Zhou, Qian Wang, Meichao Zhang, Dong Li
The Schlafen (slfn) gene family members express broadly, but the research has mainly focused on human slfn (h-slfn) and mouse slfn (m-slfn). The slfn members can be divided into three groups, and each group has its own characteristics and functions. Although the effects of slfns are still poorly understood, it has been confirmed that slfns are involved in the defense of immune system and regulate immune cells' proliferation and differentiation. In some malignant tumors, the slfn proteins can inhibit the growth and invasion of cancer cells, promote cancer cells sensibility to chemotherapeutics, and can be a promising new therapeutic target...
April 29, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28460412/mir-615-3p-inhibits-the-osteogenic-differentiation-of-human-lumbar-ligamentum-flavum-cells-via-suppression-of-osteogenic-regulators-gdf5-and-foxo1
#14
Jichao Yin, Guihua Zhuang, Yi Zhu, Xinglv Hu, Hongmou Zhao, Rongqiang Zhang, Hao Guo, Xiaochen Fan, Yi Cao
Ossification of the ligamentum flavum (OLF) is a disease of heterotopic ossification in spinal ligaments. The key of the OLF pathogenesis is the differentiation of fibroblasts into osteoblasts. In this study, we explored the role of miR-615-3p in the osteogenic differentiation of human LF cells. The expression of miR-615-3p was detected during the osteogenic differentiation of hFOB1.19 human osteoblasts, human BMSCs and human LF cells. The qPCR results showed that miR-615-3p was being decreased during the osteogenic differentiation of these cell lineages...
April 29, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28418174/hypoxia-upregulates-integrin-gene-expression-in-microvascular-endothelial-cells-and-promotes-their-migration-and-capillary-like-tube-formation
#15
Christina Befani, Panagiotis Liakos
Tissue hypoxia affects gene expression through the hypoxia-inducible transcription factors, HIF-1 and HIF-2, in both physiological and pathological angiogenesis. Angiogenesis is a complex response of endothelial cells integrating cell proliferation, migration, tube formation, and their interaction with the extracellular matrix through integrin receptors. In this report, we studied the effect of hypoxia on the angiogenic functions of human microvascular endothelial cells (HMEC-1) as well as on expression of the angiogenic integrins αν β3 , αν β5 , and α5 β1 ...
April 18, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28418110/identification-of-circulating-mirna-involved-in-meat-yield-of-korean-cattle
#16
Surim Lee, Seung-Ju Park, Jae-Kyoung Cheong, Jong-Youl Ko, Jinjong Bong, Myunggi Baik
Cattle plays an important role in providing essential nutrients through meat production. Thus, we focused on epigenetic factors associated with meat yield. To investigate circulating miRNAs that are involved with meat yield and connect biofluids and longissimus dorsi (LD) muscle in Korean cattle, we performed analyses of the carcass characteristics, miRNA array, qPCR, and bioinformatics. Carcass characteristics relative to the yield grade (YG) showed that the yield index and rib eye area were the highest, whereas the backfat thickness was the lowest for YG A (equal to high YG) cattle among the three YGs...
April 18, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28403532/temozolomide-increases-mhc-i-expression-via-nf-%C3%AE%C2%BAb-signaling-in-glioma-stem-cells
#17
Dongyong Zhang, Bo Qiu, Yunjie Wang, Yanlei Guan, Luyang Zhang, Anhua Wu
Gliomas are the most common and primary tumors of the central nervous system in adults. Temozolomide (TMZ) is the main drug used to treat glioma; however, prognosis remains poor for most patients. Glioma stem cells (GSCs) are thought to enable glioma initiation and evasion from immune surveillance; their immunogenicity can be determined by expression of major histocompatibility complex (MHC)-I. The present study investigated the effect of TMZ on MHC-I expression in GSCs. Glioma spheres were cultured in serum-free medium containing epidermal growth factor, basic fibroblast growth factor, and B27; MHC-I expression was detected by immunocytochemistry, quantitative real-time PCR, and flow cytometry...
April 12, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28403524/human-mesenchymal-stem-cells-promote-cd34-hematopoietic-stem-cell-proliferation-with-preserved-red-blood-cell-differentiation-capacity
#18
Show Xuan Lau, Yin Yee Leong, Wai Hoe Ng, Albert Wee Po Ng, Ida Shazrina Ismail, Narazah Mohd Yusoff, Rajesh Ramasamy, Jun Jie Tan
Studies showed that co-transplantation of mesenchymal stem cells (MSCs) and cord blood-derived CD34(+) hematopoietic stem cells (HSCs) offered greater therapeutic effects but little is known regarding the effects of human Wharton's jelly derived MSCs on HSC expansion and red blood cell (RBC) generation in vitro. This study aimed to investigate the effects of MSCs on HSC expansion and differentiation. HSCs were co-cultured with MSCs or with 10% MSCs-derived conditioned medium, with HSCs cultured under standard medium served as a control...
April 12, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28403518/formation-of-the-igf1r-cav1-src-tri-complex-antagonizes-trail-induced-apoptosis-in-gastric-cancer-cells
#19
Tianshu Guo, Ling Xu, Xiaofang Che, Simeng Zhang, Ce Li, Jin Wang, Jing Gong, Rui Ma, Yibo Fan, Kezuo Hou, Huiming Zhou, Xuejun Hu, Yunpeng Liu, Xiujuan Qu
Lipid rafts provide a biological platform for apoptosis induced by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). We previously reported that insulin-like growth factor 1 receptor (IGF1R) translocation into lipid rafts helped to explain TRAIL resistance. However, it was not clear whether TRAIL resistance was caused by the interaction of IGF1R with caveolin-1 (CAV1) and the non-receptor tyrosine kinase SRC in lipid rafts of gastric cancer cells. Here, we observed high IGF1R expression in TRAIL-resistant gastric cancer cells, and showed that IGF1R combined with both CAV1 and SRC in a native complex...
April 12, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28387429/cytoskeletal-mechanisms-in-positioning-of-the-second-division-spindles-and-meiotic-restitution-in-tobacco-nicotiana-tabacum-l-microsporogenesis
#20
Yuriy Vladimirovich Sidorchuk, Elena Victorovna Deineko
Microsporogenesis patterns of the polyploid (2n = 4x = 96) and diploid (2n = 2x = 48) Nicotiana tabacum L. (cv. Havana Petit line SR1) plants have been analyzed and compared. Four types of abnormal positions of the second-division spindles-tripolar, parallel, proximal, and fused-have been observed. Of these abnormalities, only tripolar (2.4%) and parallel (1.4%) spindles are observable in diploid plants. As for polyploids, the increased ploidy is accompanied by an increase in the incidence of tripolar (22...
April 7, 2017: Cell Biology International
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