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Cell Biology International

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https://www.readbyqxmd.com/read/28102640/activated-macrophages-as-key-mediators-of-capsule-formation-on-adipose-constructs-in-tissue-engineering-chamber-models
#1
Weiqing Zhan, Feng Lu
In plastic and reconstructive field, it would be much beneficial to fabricate an engineered adipose tissue substitute allowing reliable and complete fat tissue regeneration. Tissue engineering chamber (TEC) holds the promise to optimize an adipogenic configuration that is efficacious as well as reproducible. A frequently occurring complication involves the adipose tissue flap encapsulation and, effectively, its shielding, by a thick fibrous membrane, which hinders development into the proliferative stage. The reason for the deposition of the collagen capsule remains unclear...
January 19, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28102559/effect-of-bone-like-hydroxyapatite-poly-amino-acid-loaded-with-rifapentine-microspheres-on-bone-and-joint-tuberculosis-in-vitro
#2
Yuwu Liu, Dianming Jiang
Rifapentine-loaded poly(lactic-co-glycolic acid) microspheres (RPMs)-loaded bone-like hydroxyapatite/poly amino acid (BHA/PAA) is effective in curing S. aureus-induced chronic osteomyelitis. This study continues to investigate the effect of RPM-loaded BHA/PAA on the bacterial growth of Mycobacterium tuberculosis (MTB), cell proliferation and differentiation in MTB H37Rv-infected MG63 cells. Furthermore, whether Wnt/β-catenin signaling pathway was activated by RPM-loaded BHA/PAA was explored. We found the bactec growth index of H37Rv was significantly inhibited by RPM-loaded BHA/PAA...
January 19, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28074529/epigenetic-mediated-immune-suppression-of-positive-co-stimulatory-molecules-in-chemoresistant-ovarian-cancer-cells
#3
Ercan Cacan
The immunological response against cancer is a critical balance between immune-activating and immune-suppressing mechanisms. Ovarian cancer creates a suppressive microenvironment to escape immune elimination; however, the molecular mechanisms are poorly understood, and it is unclear whether chemotherapeutic drugs exert an immunoreactive or immunosuppressive effect on the tumor microenvironment. 4-1BB ligand (4-1BBL/CD157) and OX-40 ligand (OX-40L/CD252) are important regulators of effector cytotoxic T-cells activity...
January 11, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28067449/13cra-regulates-the-differentiation-of-antler-chondrocytes-through-targeting-runx3
#4
Hong-Liang Zhang, Hang Cao, Zhan-Qing Yang, Shuang Geng, Kai Wang, Hai-Fan Yu, Bin Guo, Zhan-Peng Yue
Although 13cRA is involved in the regulation of cellular proliferation and differentiation, its physiological roles in chondrocyte proliferation and differentiation still remain unknown. Here we showed that 13cRA could induce the proliferation of sika deer antler chondrocytes and expression of Ccnd3 and Cdk6. Administration of 13cRA to antler chondrocytes resulted in an obvious increase in the expression of chondrocyte marker Col II and hypertrophic chondrocyte marker Col X. Silencing of Crabp2 expression by specific siRNA could prevent the 13cRA-induced up-regulation of Col X, whereas overexpression of Crabp2 showed the opposite effects...
January 9, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28067437/the-effect-of-glutamate-on-ghrelin-release-in-mice
#5
Rakhi Chacrabati, Zhi Gong, Chika Ikenoya, Daisuke Kondo, Jeffrey M Zigman, Takafumi Sakai, Ichiro Sakata
Ghrelin is abundantly produced in the stomach. Here, we found that glutamate decreased ghrelin expression and release in ghrelin-producing cells, and decreased levels of food intake and plasma acyl-ghrelin in mice. Treatment with siRNA of G protein-coupled receptor, family C, group 5, member B (GPRC5B) in ghrelin-producing cell lines completely blocked the effect of glutamate-induced ghrelin suppression. In addition, glutamate inhibited ghrelin release via the extracellular signal-regulated kinase (ERK) activity pathway, and stimulated CREB2 mRNA expression in ghrelin-producing cell lines...
January 9, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28067431/analysis-of-undergraduate-cell-biology-contents-in-brazilian-public-universities
#6
Claudia Mermelstein, Manoel Luis Costa
The enormous amount of information available in cell biology has created a challenge in selecting the core concepts we should be teaching our undergraduates. One way to define a set of essential core ideas in cell biology is to analyze what a specific cell biology community is teaching their students. Our main objective was to analyze the cell biology content currently being taught in Brazilian universities. We collected the syllabi of cell biology courses from public universities in Brazil and analyzed the frequency of cell biology topics in each course...
January 9, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28035727/the-role-of-tgf-%C3%AE-1-during-skeletal-muscle-regeneration
#7
REVIEW
Kamila Delaney, Paulina Kasprzycka, Maria A Ciemerych, Malgorzata Zimowska
The injury of adult skeletal muscle initiates series of well-coordinated events that lead to the efficient repair of the damaged tissue. Any disturbances during muscle myolysis or reconstruction may result in the unsuccessful regeneration, characterized by strong inflammatory response and formation of connective tissue, i.e. fibrosis. The switch between proper regeneration of skeletal muscle and development of fibrosis is controlled by various factors. Among them are those belonging to the transforming growth factor β family...
December 29, 2016: Cell Biology International
https://www.readbyqxmd.com/read/28035725/cyclophosphamide-promotes-breast-cancer-cell-migration-through-cxcr4-and-matrix-metalloproteinases
#8
Chao-Ming Hung, Yi-Chiang Hsu, Tzu-Yu Chen, Chi-Chang Chang, Mon-Juan Lee
Cyclophosphamide is indicated for the treatment of cancerous diseases such as breast cancer and cervical cancer. Recent studies have shown that cyclophosphamide may induce cancer metastasis, but the cause of this unexpected adverse effect is not fully understood. In this study, we investigate the effect of cyclophosphamide on cancer cell migration, and its correlation to chemokine (C-X-C motif) receptor 4 (CXCR4), a biomarker for cancer metastasis. Two human cancer cell lines with significant difference in endogenous CXCR4 expression, the breast cancer cell line, MDA-MB-231, and the melanoma cell line, MDAMB- 435S, were treated with various concentrations of cyclophosphamide, followed by the assessment of CXCR4 expression and cell migration...
December 29, 2016: Cell Biology International
https://www.readbyqxmd.com/read/28035721/%C3%AE-carotene-treatment-alters-the-cellular-death-process-in-oxidative-stress-induced-k562-cells
#9
Handan AkÇakaya, Sabiha Tok, Fulya Dal, Suzan Adin Cinar, Rustem Nurten
Oxidizing agents (e.g. H2 O2 ) causes structural and functional disruptions of molecules by affecting lipids, proteins and nucleic acids. As a result, cellular mechanisms related to disrupted macro molecules are affected and cell death is induced. Oxidative damage can be prevented at a certain point by antioxidants or the damage can be reversed. In this work, we studied the cellular response against oxidative stress induced by H2 O2 and antioxidant-oxidant (β-carotene - H2 O2 ) interactions in terms of time, concentration and treatment method (pre-, co- and post) in K562 cells...
December 29, 2016: Cell Biology International
https://www.readbyqxmd.com/read/28032397/dna-base-excision-repair-and-nucleotide-excision-repair-synergistically-contribute-to-survival-of-stationary-phase-cells-of-the-fission-yeast-schizosaccharomyces-pombe
#10
Takanori Senoo, Shinji Kawano, Shogo Ikeda
Defects of genome maintenance may causally contribute to aging. In general, base excision repair (BER) is involved in the repair of subtle base lesions and AP sites, and bulky helix-distorting lesions are restored by nucleotide excision repair (NER). Here, we measured the chronological lifespan (CLS) of BER- and NER-deficient mutants of the fission yeast Schizosaccharomyces pombe, and observed the aging process of cells. The CLS of the nth1 (gene for DNA glycosylase/AP lyase) mutant and the rad16 (a homolog of human XPF) mutant were slightly shorter than that of the wild-type (WT) strain...
December 29, 2016: Cell Biology International
https://www.readbyqxmd.com/read/28032386/disruption-and-restoration-of-nucleolar-fc-and-dfc-during-s-phase-in-hela-cells
#11
Xin Guan, Yang Jiao, Lingling Chen, Xiliang Li, Guangbin Shang, Fengcai Wang, Yunpeng Feng, Mingda Jiao
In the higher eukaryotic nucleolus, fibrillar centers (FCs), the dense fibrillar components (DFCs), and the granular components (GCs) are functional domains structurally relatively well-defined by electron microscopy (EM). However, ultrastructural alterations in FC, DFC, and GC during the cell cycle and their associated cellular functions are still largely unclear. Based on synchronized HeLa cells, we followed the structural dynamics of nucleolus during cell cycle by EM. We found that nucleolus structure shifted from tripartite to bipartite organization and FC/DFCs were reorganized in S phase with three distinct stages: (1) In early-S phase, FC/DFC structures were disassembled...
December 29, 2016: Cell Biology International
https://www.readbyqxmd.com/read/28032380/microrna-195-5p-suppresses-osteosarcoma-cell-proliferation-and-invasion-by-suppressing-naked-cuticle-homolog-1
#12
Qiang Qu, Xiangdong Chu, Peng Wang
MiR-195-5p has been shown to play an essential role in human cancer progression. Nevertheless, the biological role of miR-195-5p in osteosarcoma development remains unclear. In this study, real-time PCR was performed to examine the miR-195-5p expression in human osteosarcoma cell lines. CCK-8 assay and Transwell assay were carried out to measure the effect of miR-195-5p on cell proliferation and invasion. Luciferase reporter assay was used to identify the targets of miR-195-5p. The results showed that miR-195-5p was significantly downregulated in osteosarcoma cells...
December 29, 2016: Cell Biology International
https://www.readbyqxmd.com/read/28032378/behavior-of-nucleolus-in-the-tobacco-male-meiocytes-involved-in-cytomixis
#13
Sergey Mursalimov, Yuriy Sidorchuk, Elena Deineko
Behavior of nucleolus during the nuclear migration between plant cells (cytomixis) is studied for the first time in the tobacco male meiosis. As is shown, the nucleolus is located in a nonrandom manner in the migrating nuclei. In the majority of cases, the nucleolus resides on the nuclear pole strictly opposite to the cytomictic channel. Owing to this localization, the nucleolus extremely rare enters the recipient cell, so that the nucleolar material is in most cases undetectable in the micronuclei formed after cytomixis...
December 29, 2016: Cell Biology International
https://www.readbyqxmd.com/read/28032372/microrna-210-is-increased-and-it-is-required-for-dedifferentiation-of-osteosarcoma-cell-line
#14
Haixia Zhang, Qing Mai, Juntao Chen
Osteosarcoma (OS) is the most common malignant bone tumor and is prevalent in adolescents. In clinical studies, miR-210 has been reported to be tightly correlated to the poor prognosis of OS. Nevertheless, its roles in OS have not been fully elucidated. In view of the central role played by OS stem cells (OSCs) in the malignant progression of OS, this study investigated the influence of miR-210 on the formation of OSCs. Our previous findings suggested that the microenvironment of bone, abundant TGF-β1 and hypoxia, could induce OS cells to dedifferentiate into OSCs...
December 29, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27987237/bladder-cancer-cells-induce-immunosuppression-of-t-cells-by-supporting-pd-l1-expression-in-tumour-macrophages-partially-through-interleukin-10
#15
Xingyuan Wang, Shaobin Ni, Qiyin Chen, Li Ma, Zhixing Jiao, Chunyang Wang, Guang Jia
Immunotherapy based on BCG vaccination is an effective treatment in bladder cancer, but a positive response is restricted to a subset of patients and for a limited period of time only. This suggests that T cells antitumour responses are effective but can become compromised in bladder cancer. To investigate the underlying mechanisms, we first identified peripheral blood monocytes and tumour macrophages using the pan-monocyte/macrophage marker CD14, and found that the PD-L1 expression on the monocytes/macrophages in bladder cancer patients was significantly higher than that in controls...
December 17, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27981676/mir-194-functions-as-a-novel-modulator-of-cellular-senescence-in-mouse-embryonic-fibroblasts
#16
Shun Xu, Bing Zhang, Yanmei Zhu, Haijiao Huang, Wenping Yang, Haiyong Huang, Hui-Ling Zheng, Xinguang Liu
MicroRNA-194 (miR-194), a typical p53 responsive miRNA, serves as a tumor suppressor similar as p53, and has been demonstrated to play an anti-proliferation role in various human cancers. In spite of the pivotal role of p53 during aging process, the knowledge of miR-194's contribution to cellular senescence is limited. We herein sought to explore the role of miR-194 in the replicative senescence and stress-induced senescence of mouse embryonic fibroblasts. Our results unraveled that, compared to young cells, miR-194 is highly expressed in senescent cells, and extra expression of miR-194 significantly triggers the replicative senescence of MEFs and H2 O2 -induced senescence of NIH/3T3 cells, while inhibition of miR-194 exhibited the opposite effect...
December 16, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27981666/skin-derived-precursors-possess-the-ability-of-differentiation-into-the-epidermal-progeny-and-accelerate-burn-wound-healing
#17
Vahid Bayati, Mohammad Reza Abbaspour, Niloofar Neisi, Mahmoud Hashemitabar
Skin-derived precursors (SKPs) are remnants of the embryonic neural crest stem cells that reside in the dermis until adulthood. Although they possess a wide range of differentiation potentials, their differentiation into keratinocyte-like cells and their roles in skin wound healing are obscure. The present study aimed to investigate the differentiation of SKPs into keratinocyte-like cells and evaluate their role in healing of third degree burn wounds. To this aim, SKPs were differentiated into keratinocyte-like cells on tissue culture plate and collagen-chitosan scaffold prepared by freeze-drying...
December 16, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27981650/rosbin-a-synthetic-small-molecule-induces-a549-cells-apoptosis-through-a-ros-mediated-pathway
#18
Ting Zhao, Wenling Jin, Hui Pan, Haizhou Li, Yang Zhao, Yang Feng
Rosbin (Thieno [2, 3-c] piperidine-3-carboxamide-2-[(3-methoxy-naphtha-lene-2-carbonyl)-amino]-6-(benzyl)-, hydrochloride), a synthetic piperidinothieno derivative compound obtained from chemical genetics screenings, significantly suppresses the viability of non-small cell lung cancer A549 cells with an IC50 of 2.05 ± 0.31 µM. It only decreases growth of non-tumour MRC-5 cells at the doses higher than 10 µM. Rosbin induces apoptosis in a dose-dependent manner by reducing the mitochondrial membrane potential (MMP) and increasing activities of caspases-3/7 and caspase-9 in A549 cells, without affecting the activity of caspase-8...
December 16, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27935220/nuclear-co-repressor-ncor-is-required-to-maintain-insulin-sensitivity-in-c2-c12-myotubes
#19
Abhijeet K Choudhary, Chinmoy S Dey
Nuclear co-repressor (NCoR) regulates peripheral insulin sensitivity; however, its role in modulating insulin sensitivity in skeletal muscle remains elusive. Present study investigated protein expression and effect of NCoR on insulin sensitivity in murine skeletal muscle cell line C2 C12 . Myotubes as compared to myoblasts of C2 C12 cells were found to be more sensitive in response to insulin as increase in insulin-stimulated phosphorylation of AKT at serine 473 residue (pAKT(S473) ) was significantly higher in myotubes...
December 9, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27925372/cyp19a1-mif-and-abca1-genes-are-targets-of-the-ror%C3%AE-in-monocyte-and-endothelial-cells
#20
Neslihan Coban, Cagri Gulec, Bilge Ozsait Selcuk, Nihan Erginel-Unaltuna
RORα is a member of nuclear receptor superfamily of transcription factors which has a vital role in the regulation of various physiological processes. Cholesterol is a known ligand of RORα and is one of the key components that take part in cardiovascular diseases such as atherosclerosis. Therefore, it is possible that RORα might have a role in the development of atherosclerosis. To test this hypothesis, we investigated the presence of novel RORα response elements (ROREs) located in the promoter of CYP19A1, MIF and ABCA1 genes...
December 7, 2016: Cell Biology International
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