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Cell Biology International

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https://www.readbyqxmd.com/read/29160602/mitochondrial-ribosome-bl34-mutants-present-diminished-translation-of-cytochrome-c-oxidase-subunits
#1
Raquel Fonseca Guedes-Monteiro, José Ribamar Ferreira-Junior, Lucas Bleicher, Francisco G Nóbrega, Antoni Barrientos, Mario H Barros
Saccharomyces cerevisiae mitoribosomes are specialized in the translation of a few number of highly hydrophobic membrane proteins, components of the oxidative phosphorylation system. Mitochondrial characteristics, such as the membrane system and its redox state driven mitoribosomes evolution through great diversion from their bacterial and cytosolic counterparts. Therefore, mitoribosome presents a considerable number of mitochondrial-specific proteins, as well as new protein extensions. In this work we characterize temperature sensitive mutants of the subunit bL34 present in the 54S large subunit...
November 21, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29150923/pkg-ii-effectively-reversed-egf-induced-protein-expression-alterations-in-human-gastric-cancer-cell-lines
#2
Hai Qian, Yan Tao, Lu Jiang, Ying Wang, Ting Lan, Min Wu, Ji Pang, Kwaku Appiah-Kubi, Yongchang Chen, Yan Wu
Epidermal growth factor receptor (EGFR) plays an important role in gastric cancer (GC) progression. Our previous data demonstrated that type II cGMP-dependent protein kinase (PKG II) could block the EGF-EGFR axis as well as down-stream signaling pathways for example, MAPK, PI3K and PLC in gastric cancer cells. However, the exact mechanisms of PKG II against cancer remain unclear. Therefore, the present work was to address the above question. Human gastric cancer cell line AGS was infected with adenoviral construct encoding cDNA of PKG II (Ad-PKG II) to up-regulate PKG II and then treated with 8-pCPT-cGMP...
November 18, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29148603/mcp2-activates-nf-%C3%AE%C2%BAb-signaling-pathway-promoting-the-migration-and-invasion-of-escc-cells
#3
Jian Zhou, Shutao Zheng, Tao Liu, Qing Liu, Yumei Chen, Doudou Tan, Rong Ma, Xiaomei Lu
MCP2, aliased CCL8, has been suggested to be implicated in the metastasis of cancer cells; however no direct evidence has been established in esophageal squamous cell carcinoma (ESCC). In our present study, to investigate the role MCP2 played in the metastasis of ESCC cells; in vitro cell co-culture system was established. Wound-healing and Transwell assays were used to evaluate the migratory and invasive variation of ESCC cells before and after treatment with recombinant human MCP2. It was shown that MCP2 was able to activate the NF-κB signaling pathway inducing the epithelial-mesenchymal transition (EMT) and promoting the migration and invasion of ESCC cells in vitro...
November 17, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29144590/metabolic-profiling-of-normal-hepatocyte-and-hepatocellular-carcinoma-cells-via-1-h-nuclear-magnetic-resonance-spectroscopy
#4
Yang Chen, Zhong Chen, Jiang-Hua Feng, Yun-Bin Chen, Nai-Shun Liao, Ying Su, Chang-Yan Zou
Hepatocellular carcinoma (HCC) causes death mainly by disseminated metastasis progression from the organ being confined. Different metastatic stages are closely related to cellular metabolic profiles. Normal hepatocyte and HepG2 cell line from low metastatic HCC were studied by NMR-based metabolomic techniques. Multivariate and univariate statistical analyses were utilized to identify characteristic metabolites from cells and cultured media. Elevated levels of acetate, creatine, isoleucine, leucine, and phenylalanine were observed in HepG2 cells, suggesting more active in gathering nutrient components along with altered amino acid metabolisms and enhanced lipid metabolism...
November 16, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29115705/co-culturing-porcine-normal-urothelial-cells-urinary-bladder-fibroblasts-and-smooth-muscle-cells-for-tissue-engineering-research
#5
Daša Zupančič, Katjuša Mrak Poljšak, Mateja Erdani Kreft
New strategies for culturing and co-culturing of the main types of urinary bladder cells are essential for successful establishment of biomimetic in vitro models, which could be applied for research into, and management of, diverse urological disorders. Porcine normal urothelial cells are available in nearly unlimited amounts and have many properties equivalent to human urothelial cells. In the present study, we established normal differentiated porcine urothelial cells in co-cultures with porcine urinary bladder normal fibroblasts and/or smooth muscle cells...
November 8, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29110400/spaghetti-a-homolog-of-human-rpap3-rna-polymerase-ii-associated-protein-3-determines-the-fate-of-germline-stem-cells-in-drosophila-ovary
#6
Dongsheng Chen, Xiaoqian Tao, Lijuan Zhou, Fuling Sun, Mingzhong Sun, Xin Fang
The Drosophila ovary provides an attractive model for studying the extrinsic or intrinsic factors that regulate the fate of germline stem cells (GSCs). Using this model, we identified a new role for Drosophila spaghetti (spag), encoding a homolog of human RNA polymerase II-associated protein 3 (RPAP3), in regulating the fate of ovarian GSCs. Results from spag knockdown and genetic mosaic studies suggest that spag functions as an intrinsic factor for GSCs maintenance. Loss of Spag by, either spag RNAi or null mutation failed to trigger apoptosis in ovarian GSCs...
November 7, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29105212/in-vitro-treatment-with-concentrated-growth-factors-cgf-and-sodium-orthosilicate-positively-affects-cell-renewal-in-three-different-human-cell-lines
#7
V Bonazza, E Borsani, B Buffoli, S Parolini, F Inchingolo, R Rezzani, L F Rodella
The aim of this study was to investigate the in vitro effect of Silicon, in the soluble form of sodium orthosilicate, combined and not with the Concentrated Growth Factors (CGF), a platelet rich preparation, on three different human cell lines of fibroblasts (NHDF), endothelial cells (HUVEC) and osteoblasts (HOBs). Each cell type, was treated with sodium orthosilicate at the final concentration of 0,5 mM and 1 mM, CGF, and sodium orthosilicate combined with CGF, for 72 hours. At the end of the experimental period, the in vitro effect on cell growth, proliferation and metabolic activity was evaluated by performing a simple cell count, using an automated cell counter and by evaluating the expression of the intracellular proliferation marker Ki-67, using Fluorescence-activated cell sorting (FACS) analysis...
November 4, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29087604/sodd-promotes-glucose-uptake-of-colorectal-cancer-cells-via-akt-pathway
#8
Rui Zhang, Jibin Li, Xiaofei Yan, Keer Jin, Wenya Li, Jian Xu, Jian Zhao, Jinghui Bai, Xiaohui Su
Altered glucose metabolism has been found in a variety of malignant diseases and recognized as one of the hallmarks of cancer. However, the molecular mechanism behind it is far from well understood. In current study, we found silencer of death domains (SODD) regulates glucose uptake of colorectal cancer cells. When cultured under low level of glucose, SODD overexpressing RKO and SW1417 cells generated more colonies, compared with control cells, in clonogenic assay. SODD upregulation enhanced RKO and SW1417 cells viability...
October 31, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29087603/mir-29b-expression-is-associated-with-a-dexmedetomidine-mediated-protective-effect-against-oxygen-glucose-deprivation-induced-injury-to-sk-n-sh-cells-in-vitro
#9
Zhi Huang, Guoli Liu, Qingfan Zeng, Rui Gao, Shuai Zhang, Lizhou Wang, Bingjie Liu, YanLong Yu, Ansu Zhao, Rui Li, Shi Zhou, Wenfeng Yu
Ischemic cerebral stroke is a leading cause of death and long-term disability world-wide. Neuronal injury following cerebral ischemia initiates a complex series of signaling cascades that lead to neuronal cell death. MicroRNA 29b (miR-29b) has reported involvement in the pathogenic process of ischemic brain injury. Dexmedetomidine (Dex) is a highly selective α2 adrenergic receptor stimulant that exerts a protective effect on brain tissue. To determine whether Dex might directly influence miR-29b expression after an ischemic injury, human neuroblastoma SK-N-SH cells were subjected to oxygen-glucose deprivation (OGD) for the purpose of creating a neuronal injury model that mimics the effects of brain ischemia in vitro...
October 31, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29076586/cancer-and-normal-adipose-derived-mesenchymal-stem-cells-ascs-do-they-have-differential-effects-on-tumor-and-immune-cells
#10
Mahboobeh Razmkhah, Zahra Mansourabadi, Maryam Al-Sadat Mohtasebi, Abdol-Rasoul Talei, Abbas Ghaderi
Adipose-derived mesenchymal stem cells (ASCs) are known to have immunomodulatory properties through soluble factors or by direct cell-to-cell contact. This study aimed to assess the expression of HLA-G and IDO activity in breast cancer and normal ASCs and to see if ASC is capable of modulating both tumor cells and immune system cells in vitro. ASCs were enzymatically isolated from 15 breast cancer patients and 10 normal individuals. Then they were cultured, and the impact of their conditioned media on the movement of the MDA-MB-231 breast cancer cell line was studied in wound healing scratch assay...
October 27, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29076585/cell-line-cross-contamination-a-detrimetal-issue-in-current-biomedical-research
#11
Armando Rojas, Ileana Gonzalez
In an article in the issue of September of Cell Biology International (Saladino et al. 2017), authors have used the cell line ECV-304 as a "human endothelial cell line". They even declare, as stated in text, that cell line were obtained from European Collection of Cell Cultures (ECACC), accordingly to the policy of the Journal.
October 27, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29068103/desipramine-induced-lysosomal-vacuolization-is-independent-of-autophagy
#12
Hirofumi Sawai
Desipramine, a commonly used antidepressant drug, induced cytosolic vacuolization in L929 cells. The level of LC3-II was elevated and that of p62 was reduced in desipramine-treated L929 cells, indicating the induction of autophagy by desipramine. Surprisingly, massive vacuolization was observed in desipramine-treated L929 cells in the presence of LY294002, an inhibitor of autophagy. On the other hand, bafilomycin A1, an inhibitor of vacuolar type H(+) ATPase, almost completely inhibited vacuolization in desipramine- or desipramine/LY294002-treated L929 cells...
October 25, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29068101/isolation-and-characterisation-of-human-mesenchymal-stem-cells-derived-from-synovial-fluid-by-magnetic-activated-cell-sorting-macs
#13
Zhaofeng Jia, Yujie Liang, Xiao Xu, Xingfu Li, Qisong Liu, Yangkan Ou, Li Duan, Weimin Zhu, Wei Lu, Jianyi Xiong, Daping Wang
Mesenchymal stem cells (MSCs) are the primary source of cells used for cell-based therapy in tissue engineering. Mesenchymal stem cells (MSCs) are found in synovial fluid, a source that could be conveniently used for cartilage tissue engineering. However, the purification and characterisation of SF-MSCs has been poorly documented in the literature. Here we outline an easy-to-perform approach for the isolation and culture of MSCs derived from human synovial fluid (hSF-MSCs). We have successfully purified hSF-MSCs using magnetic activated cell sorting (MACS) sorting using the MSC surface marker, CD90...
October 25, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29068100/upregulated-osterix-expression-elicited-by-runx2-and-dlx5-is-required-for-the-accelerated-osteoblast-differentiation-in-pp2a-c%C3%AE-knockdown-cells
#14
Di Yang, Hirohiko Okamura, Lihong Qiu
Serine/threonine protein phosphatase 2A (PP2A) is involved in regulating various physiological processes including cell cycle, growth, apoptosis, and signal transduction. Osteoblast differentiation is controlled by main bone specific transcription factors including Osterix, distal-less homeobox 5 (Dlx5), and Runt-related transcription factor 2 (Runx2). We previously reported that knockdown of PP2A Cα increases the expression of Osterix, leading to the accelerated osteoblast differentiation through the upregulation of bone-related genes...
October 25, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29064609/the-micronome-of-mesenchymal-stromal-cells-is-partially-responsive-to-inflammation
#15
Hussein Fayyad-Kazan, Mohammad Fayyad-Kazan, Makram Merimi, Nathalie Meuleman, Dominique Bron, Laurence Lagneaux, Mehdi Najar
Mesenchymal stromal cells (MSCs) display a special immunological profile that allows their potential use as immunotherapeutic cells. Nowadays, Foreskin (FSK) represents a valuable reservoir of MSCs with International Society for cellular Therapy (ISCT) compliant criteria and relevant functional properties. However, their mode of action is poorly understood and needs to be more elucidated to optimize their therapeutic use. Because microRNAs (miRNAs) act as key regulators in a wide variety of biological processes, we decided to establish the micronome of FSK-MSCs, the influence of inflammation and the predicted target pathways...
October 24, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29064603/histone-deacetylase-inhibitor-stimulates-e2-and-p4-secretion-in-sika-deer-ovarian-granulosa-cells-at-a-moderate-dose
#16
Mingjie Xing, Xiumin Chen, Xiaoxia Li, Yifeng Yang, Xiaoxu Wang, Xinyan Cao, Hailong Xue, Shiyong Wang, Yunfei Diao, Weigang Zhao, Meng Zhao, Xuezhe Cui, Tong Chang, Baozeng Xu, Haijun Wei
The histone deacetylase inhibitor (HDACi) and tumor suppressor play an important role in genome reorganization and epigenetic regulation. In this study, granulosa cells (GCs) isolated from sika deer ovaries were cultured and treated with different concentrations of trichostatin A (TSA) for 48 h. It was found that TSA inhibited GCs proliferation and induced GCs apoptosis by upregulating expression of BAX, meanwhile, downregulating expression of GLUT3, GLUT8, BCL-XL. In addition, TSA caused cell cycle arrest at the G1 and G2/M phase accompanied by reducing expression of Cyclin D2 and CDK4...
October 24, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29064598/contribution-to-mitochondrial-research-in-brazil-10-th-anniversary-of-the-mitomeeting
#17
Anibal E Vercesi, Helena C F Oliveira
This issue comprises communications, articles and mini-reviews authored by speakers of the 10th Mitomeeting, held in Guapé, MG (Brazil), April 27-30, 2017. Mitomeetings gather people interested in all aspects of mitochondrial biology in diverse species, including protists, animals, plants and fungi.
October 24, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29064597/mir-23b-3p-and-mir-125b-5p-downregulate-apo-a-expression-by-targeting-ets1-in-hepg2-cells
#18
Jun-Fa Zeng, Zhao-Lin Zeng, Kai Zhang, Yue Zhao, Ya-Mi Liu, Jiao-Jiao Chen, Hai Tong, Dang-Heng Wei, Zhi-Sheng Jiang, Zuo Wang
High concentrations of plasma lipoprotein(a) [Lp(a)] have been inferred to be an independent risk factor for cardiovascular and cerebrovascular diseases, such as coronary artery diseases, restenosis, and stroke. Apolipoprotein(a) [apo(a)] is one of the most important components of Lp(a) and contributes greatly to the increased concentration of plasma Lp(a). As a critical positive transacting factor of apo(a) gene, Ets1 has been proven as a target gene of several miRNAs, such as miR-193b, miR-125b-5p, miR-200b, miR-1, and miR-499...
October 24, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29052919/oamdp-a-novel-podophyllotoxin-derivative-induces-apoptosis-cell-cycle-arrest-and-autophagy-in-hepatoma-hepg2-cells
#19
Jie Ren, Yu Liu, Lixia Li, Yuexin Zhao, Zhongyu Li, Chao Wu, Lin Chen, Kun Hu
4β-(1,3,4-oxadiazole-2-amino-5-methyl)-4-deoxypodophyllotoxin (OAMDP), a novel podophyllotoxin derivative, has demonstrated potent anti-tumor activity with significant cytotoxic effect. Here we report the anti-proliferative effect of OAMDP, for which OAMDP could suppress the proliferation of HepG2 cells (human hepatoma cell line) in a dose- and time-dependent manner. After treating with OAMDP, cell apoptosis was confirmed by Annexin V-FITC/PI double staining assay. Furthermore, flow cytometry analysis revealed the loss of mitochondrial membrane potential and the increase of intracellular reactive oxygen species in HepG2 cells...
October 20, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29052908/hbxag-suppresses-cell-apoptosis-and-promotes-the-secretion-of-placental-hormones-in-human-placental-trophoblasts-via-activation-of-the-egfr-akt-pathway
#20
Weimin Wang, Guiqin Bai, Yuting Zhang, Ting Zhang, Chen Li, Yongxing Yuan, Sixue Liu, Caili Wang
The aim of this study is to investigate the role of Hepatitis B virus x (HBx) in the growth and secretion of human placental trophoblasts. Firstly, placenta tissues were collected from pregnant HBV carriers with various viral loads. The results of immunohistochemical technique showed that the HBx protein and pEGFR protein levels were both markedly increased with the viral load elevation. Then, a placental trophoblast cell strain (JEG-3-HBx), which stablly expressed HBx mRNA and protein, was established with the pcDNA-HBx transfection followed by the G418 selection...
October 20, 2017: Cell Biology International
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