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Cell Biology International

Julia Carnaz Benincasa, Luiz Henrique Freitas de Filho, Giane Daniela Carneiro, Micheli Severo Sielski, Selma Giorgio, Claudio Chrysostomo Werneck, Cristina Pontes Vicente
Hyperbaric oxygen is a clinical treatment that contributes to wound healing by increasing fibroblasts proliferation, collagen synthesis, and production of growth factors, inducing angiogenesis and inhibiting antimicrobial activity. It also has been shown that hyperbaric oxygen treatment (HBO), through the activation of nitric oxide synthase promotes an increase in the nitric oxide levels that may improve endothelial progenitor cells (EPC) mobilization from bone marrow to the peripheral blood and stimulates the vessel healing process...
October 25, 2018: Cell Biology International
Khairunnisa Ramli, Aminath Ifasha Gasim, Amir Ahmad Adham, Shariful Hassan, Law Zhe Kang, Tan Geok Chin, Azmi Baharuddin, Amaramalar Selvi Naicker, Ohnmar Htwe, Hazla Mohammad Haflah, Idrus Ruszymah Bh, Shalimar Abdullah, Ng Min Hwei
In peripheral nerve injuries, Schwann cells (SC) play pivotal roles in regenerating damaged nerve. However, the use of SC in clinical cell-based therapy is hampered due to its limited availability. In this study, we aim to evaluate the effectiveness of using an established induction protocol for human bone marrow derived-MSC (hBM-MSCs) transdifferentiation into a SC lineage. A relatively homogenous culture of hBM-MSCs was first established after serial passaging (P3), with profiles conforming to the minimal criteria set by International Society for Cellular Therapy (ISCT)...
October 25, 2018: Cell Biology International
Wenjun Li, Chaoyang Wang, Xiaonu Peng, Hongwei Zhang, Haibo Huang, Huimin Liu
Our study aimed to explore the function of cystic fibrosis transmembrane conductance regulator (CFTR) in esophageal cancer. Twenty patients with esophageal squamous cell carcinoma (ESCC) and 20 patients with esophageal adenocarcinoma (EA) were enrolled in this study. The levels of CFTR and NF-κB in tumor tissues and adjacent normal tissues were detected, respectively. The expression of CFTR were detected by qRT-PCR and western blot in normal esophageal cell line, esophagus squamous cell, carcinoma cell lines and EA cell lines, respectively...
October 25, 2018: Cell Biology International
Feng Zhang, Xin Wan, Yi-Zhi Cao, Dong Sun, Chang-Chun Cao
Klotho is a protein primarily expressed in renal tubular epithelial cells. Studies have suggested that Klotho is an antiaging protein that reduces renal fibrosis after acute kidney injury (AKI) and inhibits stem cell senescence. Bone marrow mesenchymal stem cells (BMSCs) have consistent proliferation ability and multidirectional differentiation ability and have been used to treat tissue injury. Thus, we hypothesized that Klotho expressed in BMSCs could increase the renal protective effects of BMSCs. To verify the hypothesis, we isolated BMSCs from C57BL/6 mice, transfected them with Klotho-GFP-adenovirus and investigated the change in BMSC proliferation...
October 25, 2018: Cell Biology International
Lívia Prometti Rezende, Maria Raquel Unterkircher Galheigo, Breno Costa Landim, Amanda Rodrigues Cruz, Françoise Vasconcelos Botelho, Renata Graciele Zanon, Rejane Maira Góes, Daniele Lisboa Ribeiro
Recent studies have been trying to find out how diet and metabolic changes such as dyslipidaemia, hyperglycaemia and hyperinsulinaemia can stimulate cancer progression. This investigation aimed to evaluate the effect of high concentrations of fatty acids and/or glucose in tumour prostate cells, focusing on the proliferation/migration profile and oxidative stress. PC3 cells were treated with high concentration of saturated fatty acid (palmitate, 100µM), glucose (220 mg/dL) or both for 24 or 48 h. Results demonstrated that PC3 cells showed a significant increase in proliferation after 48 h of treatment with glucose and palmitate + glucose...
October 24, 2018: Cell Biology International
Vikrant R Patil, Avinash H Kharat, Deepak G Kulkarni, Supriya M Kheur, Ramesh R Bhonde
Dental pulp stem cells have emerged as a preferred source of mesenchymal stem cells, because of its easy availability and high stem cell content. Dental pulp is a specific fibrous tissue that contains heterogeneous populations of odontoblasts, fibroblasts, pericytes, progenitors, stem cells, leukocytes and neuronal cells. In this study, we propose sustained explant culture as a simple, economical and efficient process to isolate dental pulp stem cells from human Dental pulp Tissue. Historically explant cultures were used to get fibroblast cells from embryonic chick heart using plasma clot cultures...
October 24, 2018: Cell Biology International
Rifu Xu, Ning Qin, Xiaoxing Xu, Xue Sun, Xiaoxia Chen, Jinghua Zhao
The SLIT/ROBO pathway has been implicated in prehierarchical follicular development of hen ovary by an intrafollicular autocrine and/or paracrine fashion. SLIT3, one of the key components of the SLIT/ROBO family, serves as a ligand that potentially interacts with the four receptors, ROBO1, ROBO2, ROBO3 and ROBO4. But the exact roles and regulatory mechanism of SLIT3 in chicken ovarian follicle development remain largely unclear. The present study was conducted to investigate the potential roles and molecular regulation of SLIT3 in granulosa cell (GC) proliferation, differentiation and follicle selection within the prehierarchical follicles of hen ovary...
October 4, 2018: Cell Biology International
Yong Cui, Yanjie Wang, Danyu Zhao, Xiaofan Feng, Lin Zhang, Chun Liu
Neuroinflammation is closely related with the pathogenesis and progress of neurodegenerative diseases including Alzheimer's disease (AD). Loganin, an iridoid glycoside obtained from traditional Chinese medicine Cornus officinalis, has properties of inhibiting inflammation and improving memory. The present study was aimed to investigate effects of loganin on Aβ-induced inflammation and to explore the underlying mechanisms. BV-2 microglia cells were stimulated with 10 µM Aβ1-42 for 24 h to induce inflammatory damage...
October 4, 2018: Cell Biology International
Fereshteh Mehdipour, Mahboobeh Razmkhah, Somayeh Rezaeifard, Mandana Bagheri, Abdol-Rasoul Talei, Behzad Khalatbari, Abbas Ghaderi
The immune-modulatory effect of adipose-derived stem cells (ASCs) on B cells in cancer has not been well elucidated. Herein, the interaction between B cells and ASCs isolated from the breast fat of either normal (nASCs) or breast cancer women (cASCs) was investigated. B cells derived from breast tumor draining lymph nodes were co-cultured with nASCs or cASCs and B cells proliferation was assessed in direct and transwell assays. Moreover, B cells were co-cultured with cASCs, nASCs or mesenchymal stromal cells of the tumor tissue (TSCs) and B cell cytokine production was assessed using flow cytometery...
October 4, 2018: Cell Biology International
Yanwen Xu, Chuanpeng Liao, Renli Liu, Jing Liu, Zhongping Chen, Huafu Zhao, Zongyang Li, Lei Chen, Changpeng Wu, Hui Tan, Wenlan Liu, Weiping Li
Alternatively activated (M2) macrophage promotes glioma progression and immune escape as the most immunocyte in glioma microenvironment. Finding out the key protein regulating M2 macrophage polarization is necessary for improving treatment. Whether immunity related GTPase M (IRGM) is involved in glioma development and M2 macrophage polarization is unknown. IRGM and M2 macrophage marker CD206 expression were examined using immunohistochemistry among 35 glioma and 11 non-cancerous brain specimens. We found IRGM scores were positively correlated with CD206 scores in glioma specimens and monocyte proportion in blood samples...
October 4, 2018: Cell Biology International
G Chen, Q H Tao
Klotho beta (Klb), a single-pass transmembrane protein, has been described as a co-receptor for endocrine FGFs, such as FGF15/19 and FGF21, to regulate critical metabolic processes in multiple organs and tissues in adult mice. However, its function during early embryonic development remains largely unknown. In this paper, we evaluated for the first time the expression of klb mRNA during early development of Xenopus laevis by RT-PCR and whole mount in situ hybridization. RT-PCR experiments showed that the expression of klb was initially detected at late gastrula stage followed by a quick increasing and continued expression throughout embryonic development...
September 27, 2018: Cell Biology International
Junya Kaneko, Toshinori Okinaga, Wataru Ariyoshi, Hisako Hikiji, Seiko Fujii, Kenjiro Iwanaga, Kazuhiro Tominaga, Tatsuji Nishihara
Histone deacetylase has attracted much attention as an epigenetic factor, and the modulation of histone and transcription factor acetylation status is important for regulating gene expression. Moreover, histone deacetylase inhibitors are involved in cellular growth and differentiation. In the present study, we examined the effects of Ky-2, a hybrid-compound HDAC inhibitor, on inflammatory reactions and the polarization of macrophages in vitro. Human monocyte-like THP-1 cells were polarized to macrophage-like cells using phorbol 12-myristate 13-acetate, and then polarized to M1 macrophages with LPS...
September 20, 2018: Cell Biology International
Sanaz Hasani, Mahdi Eskandarian Boroujeni, Abbas Aliaghaei, Kaveh Baghai, Amin Rostami
Neural differentiation involves drastic morphological alterations, essentially performed by a cell-homeostasis maintaining process known as autophagy. Here, we used the cocktail of choroid plexus epithelial cell-conditioned medium (CPEC-CM) and 15% knockout serum (KS) to induce human adipose-derived mesenchymal stem cells (hASCs) into tyrosine hydroxylase (TH)-positive neuron like cells. We showed that upon this induction, autophagy pathway was transcriptionally triggered. The expression levels of autophagy markers mTOR, BECN1, and MAP1LC3 were evidently changed throughout the dopaminergic (DAergic) differentiation of hASCs, highlighting the critical role of autophagy in this process at the level of transcription...
September 20, 2018: Cell Biology International
Túlio Almeida de Hermes, Larissa Akemi Kido, Aline Barbosa Macedo, Daniela Sayuri Mizobuti, Luiz Henrique Rapucci Moraes, Marco Cesar Somazz, Valéria Helena Alves Cagnon, Elaine Minatel
Physical exercise promotes increased muscle damage in the mdx mice, the experimental model of Duchenne muscular dystrophy. Studies suggest that the estrogen level in females makes them less susceptible to muscle injuries. The aim of this study was to characterize the diaphragm (DIA) muscle response to physical exercise in male and female mdx mice. The animals were divided into 4 groups: female sedentary mdx; male sedentary mdx; female mdx submitted to exercise; and male mdx mice submitted to exercise. Blood samples were used to determine creatine kinase (CK)...
September 20, 2018: Cell Biology International
Marcelo Martinez, Isabela M U Rossetto, Fermino Sanches Lizarte Neto, Luiz F Tirapelli, Daniela P C Tirapelli, Luiz Gustavo A Chuffa, Valeria H A Cagnon, Francisco E Martinez
Ethanol alters motricity, learning, cognition, and cellular metabolism in the cerebellum. The combination of ethanol with caffeine by consuming "energy drinks" is becoming increasingly popular among young people. We analyzed the use of ethanol and caffeine on apoptosis in the cerebellum of UChB rats. The adult rats were divided into three groups (n = 14/group): UChB group: rats fed with 1:10 (v/v) ethanol ad libitum (free choice for water or ethanol) drinking from >1.9 mL of ethanol/kg body weight/day, Control group and UChB/caffeine group (free choice for water or ethanol + caffeine 300 mg/L)...
September 11, 2018: Cell Biology International
Yiwen Yang, Tao Wang, Degao Chen, Qizhao Ma, Yanjiang Zheng, Shiping Liao, Yufang Wang, Ji Zhang
Colorectal cancer (CRC) is the third most common type of cancer, and its incidence and mortality are markedly increasing worldwide. Oncogenic mutations of KRAS occur in up to 40% of CRC cases and pose a great challenge in the treatment of the disease. Quercetin is a dietary flavonoid that exerts anti-oxidant, anti-inflammatory and anti-cancer properties. The current study investigated the anti-proliferative effect of quercetin on CRC cells harboring mutant or wild-type KRAS. The effect of quercetin on cell viability was investigated by MTT and colony formation assays, and apoptosis was detected using flow cytometry by labeling cells with Annexin V-FITC...
September 11, 2018: Cell Biology International
Bo Yu, Yanan Zhao, Hongli Zhang, Di Xie, Wei Nie, Kaiyao Shi
MicroRNA-143-3p (miR-143-3p) is involved in the initiation of inflammatory response and the progression of cardiovascular diseases. Myocardial hypertrophy is a common symptom in numerous cardiovascular diseases. In the current study, we attempted to demonstrate the role of miR-143-3p in the development of myocardial hypertrophy by focusing on its association with inflammation. Myocardial hypertrophy was induced by transverse aortic constriction (TAC) method in vivo and by H2 O2 administration in vitro. The expression status of miR-143-3p and downstream effectors were detected in animal heart tissues and H9c2 cells...
September 11, 2018: Cell Biology International
Yanan Duan, Yinghui Dong, Rongguang Dang, Zhihui Hu, Ye Yang, Yueyun Hu, Jianxin Cheng
Ovarian cancer is one of the most common gyneacologic malignancies, with high morbidity and high mortality. Hsa-miR-122-5p (miR-122) has been reported with tumor-suppressing roles in various cancers. In this study, miR-122 was overexpressed in ovarian cancer cells, and phenotypic experiments demonstrated that miR-122 inhibited migration and invasion in SKOV3 and OVCAR3 cells. MiR-122 also suppressed epithelial mesenchymal transition (EMT), evidenced by expression changes of E-cadherin, vimentin, matrix metalloproteinase (MMP)2, and MMP14...
August 23, 2018: Cell Biology International
Muhammad Nihad As, Rucha Deshpande, Vaijayanti P Kale, Ramesh R Bhonde, Savita P Datar
Angiogenesis, the process of new blood vessel formation from pre-existing vessels, is essential for growth and development. Development of drugs that can accelerate or decelerate angiogenesis in the context of various diseases requires appropriate preclinical screening. As angiogenesis involves complex cellular and molecular processes, in vivo studies are superior to in vitro investigations. Conventional in vitro, in vivo, and ex ovo models of angiogenesis are time consuming and tedious, and require sophisticated infrastructure for embryo culture...
August 23, 2018: Cell Biology International
Weiwei Xi, Xuming Zhao, Meijun Wu, Wenjuan Jia, Hua Li
Although microRNA-155 (miR-155) is implicated in the pathogenesis of several fibrotic diseases, information regarding its functional role in renal fibrosis is limited. The current study aims to investigate the effects of miR-155 on renal fibrosis in unilateral ureteral occlusion (UUO) mice. MiR-155 level was significantly increased in renal tissues of UUO mice and TGF-β1-treated HK2 cells. Masson's trichrome staining showed that delivery of adeno-associated virus encoding miR-155 inhibitor led to a decrease in renal fibrosis induced by UUO...
November 2018: Cell Biology International
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