Inverse Liquid Solid Chromatography to Evaluate Drug Interactions with Organosilane Modified Polydimethylsiloxane for use in Body-on-a-Chip Systems.

Body-on-a-chip and organ-on-a-chip systems utilize polydimethylsiloxane (PDMS) because of the relative suitability of the material for fabrication of microfluidic channels and chambers used in these devices. However, hydrophobic molecules, especially therapeutic compounds, tend to adsorb to PDMS which may distort the dose-response curves that feed into the Pharmacokinetic/Pharmacodynamic (PK/PD) models used to translate preclinical data into predictions of clinical outcomes. Surface modification by organosilanes is one method being explored to modify PDMS, but the effect of organosilanes on drug adsorption isotherms are not well characterized. We utilized Inverse Liquid-Solid Chromatography (ILC) to characterize the adsorption parameters of the drugs acetaminophen, diclofenac, and verapamil with native PDMS and organosilane-modified (fluoropolymer (13F) and polyethylene glycol (PEG)) PDMS surfaces, to correlate the modifications to changes in drug adsorption. It was determined that the organosilane modifications significantly changed the energy of adsorption of the test drug utilizing our methodology. This article is protected by copyright. All rights reserved.