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Biotechnology Progress

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https://www.readbyqxmd.com/read/28486779/expansion-strategies-for-human-mesenchymal-stromal-cells-culture-under-xeno-free-conditions
#1
Patrícia Aparecida Tozetti, Samia Rigotto Caruso, Amanda Mizukami, Taisa Risque Fernandes, Fernanda Borges da Silva, Fabiola Traina, Dimas Tadeu Covas, Maristela Delgado Orellana, Kamilla Swiech
Choosing the culture system and culture medium used to produce cells are key steps towards a safe, scalable and cost-effective expansion bioprocess for cell therapy purposes. The use of AB human serum (AB HS) as an alternative xeno-free supplement for mesenchymal stromal cells (MSC) cultivation has increasingly gained relevance due to safety and efficiency aspects. Here we have evaluated different scalable culture systems to produce a meaningful number of umbilical cord matrix-derived MSC (UCM MSC) using AB HS for culture medium supplementation during expansion and cryopreservation to enable a xeno-free bioprocess...
May 9, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28480558/strategic-deployment-of-cho-expression-platforms-to-deliver-pfizer-s-monoclonal-antibody-portfolio
#2
John J Scarcelli, Tanya Q Shang, Tim Iskra, Martin J Allen, Lin Zhang
Development of stable cell lines for expression of large molecule therapeutics represents a significant portion of the time and effort required to advance a molecule to enabling regulatory toxicology studies and clinical evaluation. Our development strategy employs two different approaches for cell line development based on the needs of a particular project - a random integration approach for projects where high level expression is critical, and a site-specific integration approach for projects in which speed and reduced employee time spend is a necessity...
May 8, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28480535/integrated-continuous-bioprocessing-economic-operational-and-environmental-feasibility-for-clinical-and-commercial-antibody-manufacture
#3
James Pollock, Jon Coffman, Sa V Ho, Suzanne S Farid
This paper presents a systems approach to evaluating the potential of integrated continuous bioprocessing for monoclonal antibody (mAb) manufacture across a product's lifecycle from pre-clinical to commercial manufacture. The economic, operational and environmental feasibility of alternative continuous manufacturing strategies were evaluated holistically using a prototype UCL decisional tool that integrated process economics, discrete-event simulation, environmental impact analysis, operational risk analysis and multi-attribute decision-making...
May 8, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28470866/cho-cells-engineered-for-fluorescence-read-out-of-cell-cycle-and-growth-rate-in-real-time
#4
Grischa Fuge, Yaeseong Hong, Kristoffer Riecken, An-Ping Zeng, Uwe Jandt
For efficient production of recombinant proteins by mammalian cells in a bioreactor, optimal growth rates are required and represent the most important process parameter. We present the first successful attempt to monitor the growth behavior and cell cycle state of a mammalian production relevant cell line under bioreactor cultivation conditions up to 1.2 l, utilizing a fluorescent read-out without the need of additional staining or marking. For this purpose, we developed two new production relevant cell line derivatives (CHO-K1 FUCCI CM & CHO-K1 FUCCI CN) and corresponding analytical methods...
May 4, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28445603/alternating-flow-filtration-as-an-alternative-to-internal-spin-filter-based-perfusion-process-impact-on-productivity-and-product-quality
#5
María Bosco, Cristian Paillet, Laura Mauro, Ignacio Amadeo, Eduardo Orti, Guillermina Forno
No abstract text is available yet for this article.
April 26, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28445602/identification-of-a-novel-mirna-that-increases-transient-protein-expression-in-combination-with-valproic-acid
#6
Hermann-Josef Meyer, Dorothea Reilly, Scott E Martin, Athena W Wong
Transient gene expression in mammalian cells is an efficient process to produce recombinant proteins for various research applications and large molecule therapeutics development. For the first time, we report a screen to identify human microRNAs (miRNAs) that increase titers after polyethylenimine (PEI) mediated transient transfection of a HEK293 cell line. From a library of 875 miRNAs, we identified 2 miRNAs, miR-26a-5p and miR-337-5p, that increased human IgG1 (huIgG1) yields by 50% and 25%, respectively...
April 26, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28445599/growth-engineering-of-synechococcus-elongatus-pcc-7942-for-mixotrophy-under-natural-light-conditions-for-improved-feedstock-production
#7
Aditya Sarnaik, Reena Pandit, Arvind Lali
Synechococcus elongatus PCC 7942 has been widely explored as cyanobacterial cell factory through genetic modifications for production of various value-added compounds. However, successful industrial scale-ups have not been reported for the system predominantly due to its obligate photoautotrophic metabolism and use of artificial light in photobioreactors. Hence, engineering the organism to perform mixotrophy under natural light could serve as an effective solution. Thus, we applied a genetically engineered strain of Synechococcus elongatus PCC 7942 expressing heterologous hexose transporter gene (galP) to perform mixotrophy under natural light in a temperature controlled environmental chamber (EC)...
April 26, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28440060/a-practical-approach-in-bioreactor-scale-up-and-process-transfer-using-a-combination-of-constant-p-v-and-vvm-as-the-criterion
#8
Sen Xu, Linda Hoshan, Rubin Jiang, Balrina Gupta, Eric Brodean, Kristin O'Neill, T Craig Seamans, John Bowers, Hao Chen
Bioreactor scale-up is a critical step in the production of therapeutic proteins such as monoclonal antibodies (MAbs). With the scale-up criterion such as similar power input per volume or O2 volumetric mass transfer coefficient (kL a), adequate oxygen supply and cell growth can be largely achieved. However, CO2 stripping in the growth phase is often inadequate. This could cascade down to increased base addition and osmolality, as well as residual lactate increase and compromised production and product quality...
April 25, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28440059/production-of-itaconic-acid-from-pentose-sugars-by-aspergillus-terreus
#9
Badal C Saha, Gregory J Kennedy, Nasib Qureshi, Michael J Bowman
Itaconic acid (IA), an unsaturated 5-carbon dicarboxylic acid, is a building block platform chemical that is currently produced industrially from glucose by fermentation with Aspergillus terreus. However, lignocellulosic biomass has potential to serve as low cost source of sugars for production of IA. Research needs to be performed to find a suitable A. terreus strain that can use lignocellulose derived pentose sugars and produce IA. One hundred A. terreus strains were evaluated for the first time for production of IA from xylose and arabinose...
April 25, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28440054/a-new-approach-to-obtain-cellulose-nanocrystals-and-ethanol-from-eucalyptus-cellulose-pulp-via-the-biochemical-pathway
#10
Thalita J Bondancia, Luiz Henrique C Mattoso, José M Marconcini, Cristiane S Farinas
The feasibility of integration of cellulosic ethanol production with the manufacture of cellulose nanofibers and cellulose nanocrystals was evaluated using eucalyptus cellulose pulp as feedstock and employing the biochemical route alone. For the enzymatic hydrolysis step, experimental central composite design (CCD) methodology was used as a tool to evaluate the effects of solids loading (SL) and enzymatic loading (EL) on glucose release and cellulose conversion. Glucose concentrations from 45 to 125 g/L were obtained after 24 h, with cellulose conversions from 35 to 96%...
April 25, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28440027/the-crispr-cas9-system-their-delivery-in-vivo-and-ex-vivo-applications-and-clinical-development-by-startups
#11
REVIEW
Minjung Song
The CRISPR/Cas9 gene editing system was originally derived from the prokaryotic adaptive immune system mediated by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated proteins (Cas). The system has been successfully applied to genome editing in eukaryotes and has contributed to remarkable advances in the life sciences, in areas ranging from agriculture to genetic disease therapies. For efficient editing and extending the influence of this system, proper delivery of its components is crucial...
April 25, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28401675/advanced-model-based-control-strategies-for-the-intensification-of-upstream-and-downstream-processing-in-mab-production
#12
Maria M Papathanasiou, Ana L Quiroga-Campano, Fabian Steinebach, Montaña Elviro, Athanasios Mantalaris, Efstratios N Pistikopoulos
Current industrial trends encourage the development of sustainable, environmentally friendly processes with minimal energy and material consumption. In particular, the increasing market demand in biopharmaceutical industry, as well as the tight regulations in product quality, necessitate efficient operating procedures that guarantee products of high purity. In this direction, process intensification via continuous operation paves the way for the development of novel, eco-friendly processes, characterized by higher productivity and lower production costs...
April 12, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28393500/enhanced-direct-ethanol-production-by-cofactor-optimization-of-cell-surface-displayed-xylose-isomerase-in-yeast
#13
Yusuke Sasaki, Toshiyuki Takagi, Keisuke Motone, Kouichi Kuroda, Mitsuyoshi Ueda
Xylose isomerase (XylC) from Clostridium cellulovorans can simultaneously perform isomerization and fermentation of d-xylose, the main component of lignocellulosic biomass, and is an attractive candidate enzyme. In this study, we optimized a specified metal cation in a previously established Saccharomyces cerevisiae strain displaying XylC. We investigated the effect of each metal cation on the catalytic function of the XylC-displaying S. cerevisiae. Results showed that the divalent cobalt cations (Co(2+) ), especially enhanced the activity by 46-fold...
April 10, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28393487/poly-3-hydroxybutyrate-co-3-hydroxyvalerate-production-from-biodiesel-by-product-and-propionic-acid-by-mutant-strains-of-pandoraea-sp
#14
Fabrício C de Paula, Jonas Contiero, Carolina B C de Paula, José Gregório C Gomez, Alexander Steinbüchel
Pandoraea sp. MA03 wild type strain was subjected to UV mutation in order to obtain mutants unable to grow on propionic acid (PA) but still able to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] from glycerol and PA at high 3HV yields. In shake flask experiments, mutant prp25 was selected from 52 mutants affected in the propionate metabolism exhibiting a conversion rate of PA into 3HV units of 0.78 gg(-1) . The utilization of crude glycerol (CG) plus PA or valeric acid resulted in a copolymer with 3HV contents varying from 21...
April 10, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28393483/upgrading-laccase-production-and-biochemical-properties-strategies-and-challenges
#15
REVIEW
Brandt Bertrand, Fernando Martínez Morales, María R Trejo-Hernández
Improving laccases continues to be crucial in novel biotechnological developments and industrial applications, where they are concerned. This review breaks down and explores the potential of the strategies (conventional and modern) that can be used for laccase enhancement (increased production and upgraded biochemical properties such as stability and catalytic efficiency). The challenges faced with these approaches are briefly discussed. We also shed light on how these strategies merge and give rise to new options and advances in this field of work...
April 10, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28393481/comparative-study-of-therapeutic-antibody-candidates-derived-from-mini-pool-and-clonal-cell-lines
#16
Lianchun Fan, Giovanni Rizzi, Kathleen Bierilo, Jun Tian, Joon Chong Yee, Reb Russell, Tapan K Das
The long journey of developing a drug from initial discovery target identification to regulatory approval often leaves many patients with missed window of opportunities. Both regulatory agencies and biopharmaceutical industry continue to develop creative approaches to shorten the time of new drug development in order to deliver life-saving medicine to patients. Generally, drug substance materials to support the toxicology and early phase clinical study can only be manufactured after creating the final Master Cell Bank (MCB) of the clonally derived cell line, which normally takes 1-2 years...
April 10, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28393480/characterization-of-mammalian-cell-culture-raw-materials-by-combining-spectroscopy-and-chemometrics
#17
Nicholas Trunfio, Haewoo Lee, Jason Starkey, Cyrus Agarabi, Jay Liu, Seongkyu Yoon
Two of the primary issues with characterizing the variability of raw materials used in mammalian cell culture, such as wheat hydrolysates, is that the analyses of these materials can be time consuming, and the results of the analyses are not straightforward to interpret. To solve these issues, spectroscopy can be combined with chemometrics to provide a quick, robust and easy to understand methodology for the characterization of raw materials; which will improve cell culture performance by providing an assessment of the impact that a given raw material will have on final product quality...
April 10, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28393478/optimisation-based-framework-for-resin-selection-strategies-in-biopharmaceutical-purification-process-development
#18
Songsong Liu, Spyridon Gerontas, David Gruber, Richard Turner, Nigel J Titchener-Hooker, Lazaros G Papageorgiou
This work addresses rapid resin selection for integrated chromatographic separations when conducted as part of a high-throughput screening (HTS) exercise during the early stages of purification process development. An optimisation-based decision support framework is proposed to process the data generated from microscale experiments in order to identify the best resins to maximise key performance metrics for a biopharmaceutical manufacturing process, such as yield and purity. A multiobjective mixed integer nonlinear programming (MINLP) model is developed and solved using the ε-constraint method...
April 10, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28380693/a-non-chromatographic-process-for-purification-of-secretory-immunoglobulins-from-caprine-whey
#19
Alexander Matlschweiger, Gottfried Himmler, Clemens Linhart, Michael Harasek, Rainer Hahn
Secretory immunoglobulins are an important antibody class being primarily responsible for immunoprotection of mucosal surfaces. A simple, non-chromatographic purification process for secretory immunoglobulins from caprine whey was developed. In the first process step whey was concentrated 30 - 40 fold on a 500 kDa membrane, thereby increasing the purity from 3% to 15%. The second step consisted of a fractionated PEG precipitation, in which high molecular weight impurities were removed first and in the second stage the secretory immunoglobulins were precipitated, leaving a majority of the low molecular weight proteins in solution...
April 5, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28371561/evaluation-of-several-protein-a-resins-for-application-to-multi-column-chromatography-for-the-rapid-purification-of-fed-batch-bioreactors
#20
Nicolas-Julian Hilbold, Xavier Le Saoût, Eric Valery, Laurence Muhr, Jonathan Souquet, Alain Lamproye, Hervé Broly
Most of the existing production capacity is based on fed batch bioreactors. Thanks to the development of more efficient cell lines and the development of high performance culture media, cell productivity dramatically increased. In a manufacturing perspective, it is necessary to clear as quickly as possible the protein A capture step in order to respect the manufacturing agenda. This paper describes the methodology applied for the design of a multi-column chromatography process with the objective of purifying as quickly as possible 1,000 and 15,000L fed-batch bioreactors...
April 3, 2017: Biotechnology Progress
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