Osmolarity of basal and reflex tears of normal dogs.

OBJECTIVE: To compare basal and reflex tear osmolarity in healthy dogs and to evaluate for correlation among tear film osmolarity, tear production, and tear fern pattern.

ANIMALS STUDIED: The population consisted of 22 healthy adult dogs.

PROCEDURES: Reflex tear osmolarity was measured in both eyes using the I-PEN®  VET osmometer 30 minutes following the Schirmer tear test (STT-1) measurement. Subsequently, two minutes following topical anesthetic application, the lacrimal lake and conjunctival fornices were dried, and 3 minutes later, basal tear osmolarity was measured. Tears were extracted from the dye-free STT-1 strip by centrifugation, placed on a glass slide, and the ferning pattern was determined by light microscopy. Comparisons between basal and reflex tears were performed with one-way ANOVA. Correlations between tear osmolarity, STT-1, and tear ferning were verified by Pearson's correlation coefficient.

RESULTS: No statistical difference was found between right and left eyes for STT-1, tear fern pattern, and osmolarity of reflex or basal tears (P < .05). There was no statistical difference between osmolarity of basal and reflex tears among individuals (P < .05). No correlation was detected between tear fern pattern and osmolarity of reflex tears. A weak positive correlation was detected between STT-1 values and osmolarity of reflex, but not basal tears.

CONCLUSIONS: The osmolarity of basal tears did not differ from that of reflex tears. A positive weak correlation exists between tear production and osmolarity of reflex tears. No correlation was detected between tear osmolarity measured by handheld osmometer and tear ferning.