Evaluation of the influence of Mirabilite on the absorption and pharmacokinetics of the ingredients in Dahuang-mudan decoction by a validated UPLC/Q-TOF-MS/MS method.

Dahuang-mudan decoction (DMD) has been widely used for disease treatment in China for 1700 years. The formula consists of Rhubarb, Moutan bark, Prunus persica, Wax gourd kernel and Mirabilite, which have been well studied by multidisciplinary approaches. However, the role of the mineral Mirabilite in DMD is unclear. The objective of this study was to investigate the effects of Mirabilite on the absorption and pharmacokinetics of the ingredients in DMD. The constituents were identified in DMD extract and the plasma of Mirabilite-DMD (MDMD, 50 g kg-1 )-treated rats and None Mirabilite-DMD (NMDMD, 50 g kg-1 )-treated rats. The plasma was also used to investigate the effects of Mirabilite on the pharmacokinetics of active ingredients in DMD using a new validated UPLC-MS/MS method. The results showed that 63 compounds were identified in the extract of DMD, 27 and 22 of which were found in the plasmas of MDMD and NMDMD-treated rats, respectively. Furthermore, the results of a pharmacokinetic study suggested that Mirabilite influenced the absorption of the five constituents by decreasing the absorption of emodin and rhein while increasing the absorption of aloe-emodin, paeoniflorin and amygdalin; the pharmacokinetic parameters, including the Tmax , Cmax , AUC0-t , MRT0-t , CLz and t1/2 of five constituents significantly changed in MDMD-treated rats compared with the NMDMD. The method validation for selectivity, precision, accuracy, matrix effect, recovery, and stability were reckoned acceptable. These findings uncover the roles of Mirabilite in DMD and demonstrate the application of scientific principles to the study of DMD in human health care.