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Biomedical Chromatography: BMC

Ting Cui, Hong-Mei Qiu, Dan Huang, Qi-Xin Zhou, Xiao-Yan Fu, Hai-Yan Li, Xin-Hui Jiang
The determination of amino acids with the actions-like neurotransmitters or modulators have been increasingly important for diagnose in many neuropsychiatric diseases. A rapid and simple high performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for simultaneous determination of seven amino acids: aspartate (Asp), glutamate (Glu), serine (Ser), glutamine (Gln), glycine (Gly), taurine (Tau) and γ-aminobutyric acid (GABA). Homoserine (Hse) was used as an internal standard (IS)...
January 18, 2017: Biomedical Chromatography: BMC
Rajneet Kaur Khurana, Satvinder Kaur, Jasleen Kaur, Bhupinder Singh
The degradation behavior of Mangiferin, under different ICH Q1A(R2) recommended stress conditions was studied using an isocratic elution with mobile phase (pH 2.4), composed of acetonitrile and 1% orthophosphoric acid (12:88 v/v) at a flow rate of 1.0 mL/min, with λmax 262 nm. It was suitably adapted for LC-MS studies by replacing with 1% acetic acid (ACN:1%acetic acid; 18:82) and pH was adjusted to 3.0. Extensive degradation was found to occur during alkaline medium stress studies at 2.31 min at λmax of 235 nm...
January 15, 2017: Biomedical Chromatography: BMC
Laurence Labat, Antonio Goncalves, Ana Rita Marques, Bénédicte Duretz, Bernard Granger, Xavier Declèves
Baclofen is actually used to manage alcohol dependence. This study describes a simple method using liquid chromatography coupled to high resolution mass spectrometry (LC-HR-MS) developed in plasma samples. This method was optimized to allow quantification of baclofen and determination of metabolic ratio of its metabolites, an oxidative deaminated metabolite of baclofen (M1) and its glucuronide form (M2). The LC-HR-MS method on Exactive® apparatus is a new developed method with all the advantages of the high resolution in full scan mode for the quantification of baclofen and detection of its metabolites in plasma...
January 14, 2017: Biomedical Chromatography: BMC
Yinan Wang, Min Zhao, Hao Ye, Yizhen Shao, Yongbo Yu, Miao Wang, Chunjie Zhao
Cortex Fraxini is an important traditional Chinese herbal medicine (TCM) used for the treatment of gout and hyperuricemia. An efficient and rapid ultra-performance liquid chromatography mass spectrometry (UPLC-MS) method was developed and validated for simultaneous quantitation of six coumarins (aesculin, fraxin, aesculetin, fraxetin , sopoletin and 7-hydroxycoumarin) in normal and hyperuricemic rats plasma after oral administration of Cortex Fraxini. The method could successfully be applied for pharmacokinetics studies...
January 13, 2017: Biomedical Chromatography: BMC
Ali Ozkan, Hassan Y Aboul-Enein, Muhittin Kulak, Recep Bindak
The present study was designed to determine the fatty acid composition and phytosterol contents of Turkish native olive cultivars namely (Kilis Yağlık and Nizip Yağlık cv.). In this context, olive fruits from thirty-four locations were sampled and then screened for their components in comparison. Fifteen different fatty acids were found in both olive oils. In the order of abundance, the most important ones were: oleic acid (18:1) > palmitic acid (16:0) > linoleic acid (18:2) > stearic acid (18:0)...
January 12, 2017: Biomedical Chromatography: BMC
Xin-Zhi Wang, Hong-Mei Wen, Chuan Chai, Wen-Ying Zhang, Ming Gao, Rui Liu, Hao Wu, Jing-Yu Liang
Peperomin E (PepE), a naturally occurring secolignan isolated from Peperomia dindygulensis, has drawn much attention recently due to its anticancer and DNA methyltransferase 1 (DNMT1) inhibitory activity. Here, a simple and sensitive ultra-fast liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of PepE in rat plasma for the first time. Samples were prepared by simple protein precipitation. Separation was performed on an XBridge(TM) C18 column using a mobile phase of acetonitrile and 0...
January 6, 2017: Biomedical Chromatography: BMC
Selvakumar Kanthiah, Kannappan Valliappan
This present study describes a specific, precise, sensitive and accurate method for simultaneous determination of hydroxyzine, loratadine, terfenadine, rupatadine and their main active metabolites cetirizine, desloratadine and fexofenadine in serum and urine by using meclizine as an internal standard. Solid phase extraction method for sample clean-up and preconcentration of analytes was carried out by using Phenomenex Strata-X-C and Strata X polymeric cartridges. Chromatographic analysis was performed on a Phenomenex cyano (150 mm x 4...
January 6, 2017: Biomedical Chromatography: BMC
Kambiz Gilany, Ahmad Mani-Varnosfaderani, Arash Minai-Tehrani, Fateme Mirzajani, Alireza Ghassempour, Mohammed Reza Sadeghi, Mehdi Amini, Hassan Rezadoost
Male factor infertility is involved in almost half of all infertile couples. Lack of the ejaculated sperm due to testicular malfunction has been reported in 6-10% of infertile, a condition named non-obstructive azoospermia (NOA). In this study, we investigated untargeted metabolomic profiling of the seminal plasma in NOA men using gas chromatography-mass spectrometry (GC-MS) and advance chemometrics. In this regard, the seminal plasma fluids of 11 NOA men with TESE-negative (AZN), 9 NOA men with TESE-positive (AZP) and 10 fertile (F) healthy men (as a control group) were collected...
January 6, 2017: Biomedical Chromatography: BMC
Shao-Ting Wang, Yan Li
A highly feasible and reliable ultra-high performance liquid chromatography tandem mass spectrometry method was presented for therapeutic drug monitoring of five anti-schizophrenic drugs (amisulpride, olanzapine, aripiprazole, paliperidone and ziprasidone) simultaneously. To meet the requirements of practical clinical usage (easy-handling, high-throughput and cost effective), the pretreatment process has been especially simplified (only including protein precipitation and mobile phase dilution steps) and the UPLC separation cycle was set within 6 minutes...
January 5, 2017: Biomedical Chromatography: BMC
Ruijian Zhong, Yan Yu, Yangbing Zheng, Weikang Chen, Guoping Zhou, Jianhong Ding, Mingming Yuan
A simple, sensitive and specific UHPLC-MS/MS method for quantification of Plantagoguanidinic acid (PGA) in rat plasma was applied to investigate the pharmacokinetic behavior in vivo, using protopine as internal standard. The chromatography was separated on a Phenomenex® Luna-C18 column (2.1 mm × 150 mm, 3.0 µm) within 7.0 min by using a mobile phase consisting of acetonitrile-0.1% formic acid solution under gradient elution at a flow rate of 0.4 mL/min. Prepared samples were monitored by multiple reaction monitoring mode, with the target fragmentions m/z 226...
January 4, 2017: Biomedical Chromatography: BMC
Jing Wang, Guoxiu Cao, Hong Wang, Hui Ye, Yunxi Zhong, Guangji Wang, Haiping Hao
Isochlorogenic acid A is widely presented in fruits, vegetables, and herbal medicines, and is characterized with anti-inflammatory, hepatoprotective and antiviral properties. However, little is known about its metabolic fate and pharmacokinetic properties. This study is thus designed to investigate the metabolic fate of isochlorogenic acid A. An analytical method based on high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC/Q-TOF MS) was established to characterize the metabolites of isochlorogenic acid A in the plasma, urine and feces of rats...
January 4, 2017: Biomedical Chromatography: BMC
Sirwan Mohammadiazar, Fatemeh Hasanli, Mehdi Maham, Somayeh Payami Samarin
Electrochemically co-deposited sol-gel/Cu nanocomposites has been introduced as a novel, simple and single step technique for preparation of solid phase microextraction coating to extract methadone (MDN) (a synthetic opioid) in urine samples. The porous surface structure of the sol-gel/Cu nanocomposite coating was revealed by scanning electron microscopy (SEM). Direct immersion-solid phase microextraction (DI-SPME) followed by HPLC-UV determination was employed. The factors influencing the SPME procedure such as the salt content, desorption solvent type, pH and equilibration time were optimized...
December 30, 2016: Biomedical Chromatography: BMC
Yulang Chi, Zhijun Wu, Yi Zhong, Sijun Dong
Tetrahydro-α-(1-methylethyl)-2-oxo-1(2H)-pyrimidineacetic acid (TPA) is a critical intermediate in the synthesis of HIV protease inhibitors. A simple and efficient method for the separation and determination of TPA enantiomers was developed. The TPA was separated into its enantiomers with an enantiomeric purity of 99% using an HPLC system equipped with a Chiralpak OD-H column. Semi-preparative HPLC enantioseparations were carried out for further enrichment of the enantiomers. The validity of this method was evaluated on the basis of its precision, accuracy, linearity and recovery...
December 26, 2016: Biomedical Chromatography: BMC
Hongli Du, Hongxia Zhao, Xueli Lai, Qishan Lin, Zhenyu Zhu, Yifeng Chai, Ziyang Lou
Depression is the predominant cause of illness and disability. We applied untargeted metabolomics using mass spectrometry to identify metabolic signatures associated with depression in serum and explored the antidepressant effects of Lilies and Rhizoma Anemarrhenae on an experimental model of chronic unpredictable mild stress (CUMS). Meanwhile metabolomics based on UHPLC-Q-TOF-MS, was used to study the change in metabolites in CUMS rat serum and to evaluate the effects of Anemarrhena Rhizoma, Lilies (alone and in combination)...
December 23, 2016: Biomedical Chromatography: BMC
Chih-Wei Chang, Yu-Ying Yeh, Li-Ching Chang, Mei-Chich Hsu, Yu-Tse Wu
Cat's claw is a large woody vine with hook-like thorns, has been traditionally used to treat inflammatory disorders in the South and Central America. In this study, a rapid, validated high performance liquid chromatographic (HPLC) method using silica monolithic column was developed for the simultaneous determination of oxindole alkaloids, namely rhynchophylline, pteropodine, isomitraphylline, and isopteropodine in Cat's claw. The ionic liquid-based microwave-assisted extraction (ILMAE), considered as an environmental-friendly and powerful tool, was firstly applied in the extraction of oxindole alkaloids...
December 23, 2016: Biomedical Chromatography: BMC
Katarzyna Stepnik, Irena Malinowska
The main aim of this study is to predict quantitatively human percutaneous absorption of chosen compounds commonly occurring in plants which can be used as medicinal extracts in drug and/or beauty industry. The most important human percutaneous descriptors i.e. logKp (logarithm of the water/skin partition coefficient) and logJmax (logarithm of the maximum flux of solutes penetrating the skin) of fatty acids and polyphenols have been determined using both in vitro and in silico methods. For in vitro determination of human percutaneous absorption, micellar liquid chromatography (MLC) based on hexadecyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS) as well as polyoxyethylene 23 lauryl ether (Brij35) has been used...
December 21, 2016: Biomedical Chromatography: BMC
Yueying Rong, Suxiang Feng, Chunwei Wu, Shumei Wang, Shengwang Liang, Dongyun Liu
Nao-Mai-Tong (NMT), a Chinese herbal formula, is used for treating ischemia cerebral apoplexy. To discover the components of NMT with potential in vivo bioactivity and investigate the differences between NMT and its single herb components, this study rigorously compared plasma samples collected from rats dosed with NMT and single-herb extracts at different times through LC-high resolution-MS/MS and data processing (Metworks). The plasma of the NMT group contained a total of 66 identified compounds (25 prototypes and 41 metabolites), including anthraquinones, triterpenoid saponins, isoflavones and phthalides...
December 15, 2016: Biomedical Chromatography: BMC
Faten Farouk, Ehab F ElKady, Hassan M E Azzazy
Therapeutic drug monitoring (TDM) of anti-epileptic drugs (AED) is a routine application. Carbamazepine (CRB) is monitored as the parent drug while oxcarbazepine (OXC) and eslicarbazepine acetate (ESL) are monitored as their active metabolite (eslicarbazepine; MHD). We have developed a UPLC-MS/MS method for determining CRB, OXC, ESL and MHD in plasma or serum with a simplified extraction protocol. The developed method detects sildenafil (SLD) which clinically interferes with AED and is likely to be co-administered in epileptic patients suffering from sexual insufficiency (60%)...
December 15, 2016: Biomedical Chromatography: BMC
Wei-Ying Chen, Xian-Zhen Zhou, Li-Lan Wu, Yun-Shan Wu, Shu-Mei Wang, Bo Liu, De-An Guo
Caesalpinia sappan L. is a traditional medicinal plant which is used for promoting blood circulation and cerebral apoplexy therapy in China. Previous reports showed that the extracts of Caesalpinia sappan L. could exert vasorelaxant activity and anti-inflammation activity. Protosappanin B is a major constituent of Caesalpinia sappan L., and showed several important bioactivities. The separation was achieved by an ACQUITY UPLC BEH Symmetry Shield RP-18 column (1.7 µm, 2.1 mm × 100 mm) column with the gradient mobile phase containing of 5 mM ammonium acetate aqueous solution and acetonitrile...
December 14, 2016: Biomedical Chromatography: BMC
Yu Yan, Huifang Zhang, Zhihui Zhang, Junke Song, Yucai Chen, Xiaobo Wang, Yangyang He, Hailin Qin, Lianhua Fang, Guanhua Du
Coptisine, one of the main components isolated from Coptidis rhizoma, has been reported to have many beneficial pharmacological effects including anti-inflammatory, anti-hypercholesterolemia, neuroprotective and cardioprotective properties. However, to date the information related to the in vivo pharmacokinetics (PK) of coptisine is very limited. The purposes of our study are to establish a fast and sensitive quantification method of coptisine using liquid chromatography-mass spectrometry (LC-MS) and evaluate the PK profile of coptisine in rats...
December 13, 2016: Biomedical Chromatography: BMC
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