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Biomedical Chromatography: BMC

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https://www.readbyqxmd.com/read/28636139/review-of-hplc-and-lc-ms-ms-assays-for-the-determination-of-various-non-steroidal-anti-androgens-nsaa-used-in-the-treatment-of-prostate-cancer
#1
REVIEW
P S Suresh, Nuggehally R Srinivas, Ramesh Mullangi
Prostate cancer is the most common cancer and one of the leading causes for cancer deaths in men. One of the commonly used approaches to treat metastatic prostate cancer was via first generation non-steroidal anti-androgens (NSAA) namely flutamide, nilutamide, bicalutamide and topilutamide. Most of the prostate cancer patients who are initially responsive develop a most aggressive form of disease called castration-resistant prostate cancer (CRPC). Second generation NSAA receptor antagonists (enzalutamide, apalutamide and darolutamide) are emerging as additional new options to treat CRPC...
June 21, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28623874/lansoprazole-sulphide-pharmacokinetics-of-this-promising-anti-tuberculous-agent
#2
Sipho Mdanda, Sooraj Baijnath, Adeola Shobo, Sanil D Singh, Glenn E M Maguire, Hendrik G Kruger, Per I Arvidsson, Tricia Naicker, Thavendran Govender
Lansoprazole (LPZ) is a commercially available proton-pump inhibitor (PPI) whose primary metabolite, lansoprazole sulphide (LPZS) was recently reported to have in vitro and in vivo activity against M. tb. It was also reported that a 300 mg kg(-1) oral administration of LPZS was necessary to reach therapeutic levels in the lung, with the equivalent human dose being unrealistic. A validated liquid chromatography-tandem mass spectrometric method (LC-MS/MS) for the simultaneous quantification LPZ and LPZS in rat plasma and lung homogenates, was developed...
June 17, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28623867/a-validated-uhplc-ms-ms-method-for-the-measurement-of-riluzole-in-plasma-and-myocardial-tissue-samples
#3
Suzanne L Parker, Yarmarly C Guerra Valero, Jeffrey Lipman, Steven Weiss, Camilla Smith, Lyndal Russell, Paul Smith, Jason A Roberts, Steven C Wallis
Through blocking the cardiac persistent sodium current, riluzole has the potential to prevent myocardial damage post cardiac bypass surgery. A sensitive UHPLC-MS/MS method was developed and validated for quantitation of riluzole and 5-methoxypsoralen in human plasma and myocardial tissue homogenate using a liquid-liquid extraction with dichloromethane. The chromatographic separation was achieved using Shimadzu Shim-pack XR-ODS III, 2.0 x 50 mm, 1.6 μm column with a gradient mobile phase comprising of methanol and ammonium acetate buffer pH 3...
June 17, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28623864/a-highly-sensitive-lc-ms-ms-method-for-simultaneous-determination-of-glycyrrhizin-and-its-active-metabolite-glycyrrhetinic-acid-application-to-a-human-pharmacokinetic-study-after-oral-administration
#4
Tsuneharu Suzuki, Michiko Tsukahara, Yuko Akasaka, Hideo Inoue
A highly sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of glycyrrhizin (GL) and its active metabolite, glycyrrhetinic acid (GA) from human plasma was validated and applied to a human pharmacokinetic study. The analytes were extracted from human plasma using an Oasis MAX cartridge and chromatographic separation was performed on an Inertsil ODS-3 column. The detection was performed using an API 4000 mass spectrometer operating in the positive electrospray ionization mode...
June 17, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28623851/quantification-of-%C3%AE-eudesmol-in-rat-plasma-using-lc-ms-ms-and-its-application-to-a-pharmacokinetic-study
#5
Ligang Jiang, Chunyang Zhang, Haiping Li
A sensitive and specific LC-MS/MS assay for determination of β-eudesmol in rat plasma was developed and validated. After liquid-liquid extraction with ethyl ether, the analyte and IS were separated on a Capcell Pak C18 column (50 × 2.0 mm, 5 μm) by isocratic elution with acetonitrile-water-formic acid (77.5:22.5:0.1, v/v/v) as the mobile phase at a flow rate of 0.4 mL/min. An ESI source was applied and operated in positive ion mode; selected reaction monitoring (SRM) scan was used for quantification by monitoring the precursor-product ion transitions of m/z 245...
June 17, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28623844/lc-ms-ms-characterization-anti-inflammatory-effects-and-antioxidant-activities-of-polyphenols-from-different-tissues-of-korean-petasites-japonicus-meowi
#6
Jin Young Choi, Kebede Taye Desta, Venu Venkatarame Gowda Saralamma, Sung Joong Lee, Soo Jung Lee, Seong Min Kim, Anjugam Paramanantham, Ho Jeong Lee, Yun-Hi Kim, Ho-Chul Shin, Jae-Han Shim, Sung Chul Shin, Gon-Sup Kim, A M Abd El-Aty
The Korean Petasites japonicus is a perennial plant used in folk medicine as a remedy for many diseases and popularly consumed as spring greens. Ten polyphenols were characterized from the leaves, stems, and roots of this plant via high-performance liquid chromatography-tandem mass spectrometry. Individual polyphenols were quantified for the first time using calibration curves of six structurally related external standards. Validation data indicated that correlation coefficients (R(2) ) were ≥ 0.9702 for all standards...
June 17, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28623841/development-of-a-hplc-method-for-determination-of-four-uv-filters-in-sunscreen-and-its-application-to-skin-penetration-studies
#7
Carla Souza, Patrícia M B G Maia Campos
This study describes the development, validation and application of a high-performance liquid chromatography (HPLC) method for the simultaneous determination of the in vitro skin penetration profile of four UV filters on porcine skin. Experiments were carried out on a gel-cream formulation containing the following UV filters: diethylamino hydroxybenzoyl hexyl benzoate (DHHB), bis-ethylhexyloxyphenol methoxyphenyl triazine (BEMT), methylene bis-benzotriazolyl tetramethylbutylphenol (MBBT) and ethylhexyl triazone (EHT)...
June 17, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28622708/pharmacokinetic-comparison-of-two-phenolic-acids-after-oral-administration-of-typhae-pollen-to-normal-rats-and-rats-with-acute-cold-blood-stasis
#8
Yingni Pan, Wenjie Zhang, Wei Zhang, Xuewei Bai, Shumeng Ren, Jiang Zheng, Dongmei Wang, Xiaoqiu Liu
Typhae Pollen (TP), dried pollen of Typha angustifolia L., Typha orientalis Presl or other plants of the same genus (Typhaeceae), has effect of activating the circulation to cure blood stasis in traditional Chinese Medicine (TCM). The purpose of this study was to set up an ultra-high performance liquid chromatography method that could determine p-hydroxybenzoic acid and vanillic acid simultaneously in rat plasma, and to compare their pharmacokinetics in normal rats and rats with acute cold blood stasis, and further to investigate the influence of different dosages of oral administration...
June 16, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28621883/determination-of-the-serine-palmitoyl-transferase-inhibitor-myriocin-by-electrospray-and-q-trap-mass-spectrometry
#9
Giuseppe Matteo Campisi, Paola Signorelli, Jessica Rizzo, Claudio Ghilardi, Jacopo Antognetti, Anna Caretti, Jelena S Lazarevic, Enrica Strettoi, Elena Novelli, Riccardo Ghidoni, Federico Maria Rubino, Rita Paroni
Myriocin, is a potent inhibitor of serine-palmitoyl-transferase, the first and rate-determining enzyme in the sphingolipids biosynthetic pathway. This study developed, validated and applied a LC-MS/MS method to measure Myriocin in minute specimens of animal tissue. The chemical analog 14-OH-Myriocin is used as the internal standard. The two molecules are extracted from the tissue homogenate by solid-phase extraction, separated by gradient reverse-phase liquid chromatography and measured by negative ion electrospray mass spectrometry in the triple quadrupole...
June 16, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28621487/simultaneous-quantification-of-imatinib-and-its-main-metabolite-n-demethyl-imatinib-in-human-plasma-by-liquid-chromatography-tandem-mass-spectrometry-and-its-application-to-therapeutic-drug-monitoring-in-patients-with-gastrointestinal-stromal-tumor
#10
Wei Zhuang, Hai-Bo Qiu, Xin-Meng Chen, Xiu-Hong Yuan, Li-Fang Yang, Xiao-Wei Sun, Xiao-Jun Zhou, Min Huang, Xue-Ding Wang, Zhi-Wei Zhou
AIM: To improve and validate a more stable and less time-consuming method based on liquid chromatography and tandem mass spectrometry (LC- MS/MS) for the quantitative measurement of Imatinib and its metabolite N-demethyl-Imatinib (NDI) in human plasma. METHODS: Separation of analytes was performed on a Waters XTerra RP18 column (50mm×2.1 mm i.d., 3.5 μm) with a mobile phase consisting of methanol/acetonitrile/ water (65:20:15, v/v/v) with 0.05% formic acid at a flow-rate of 0...
June 16, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28618092/a-platinized-stainless-steel-fiber-with-in-situ-coated-polyaniline-polypyrrole-graphene-oxide-nanocomposite-sorbent-for-headspace-solid-phase-microextraction-of-aliphatic-aldehydes-in-rice-samples
#11
Alireza Ghiasvand, Afagh Nasirian, Samira Koonani, Kolsoum Nouriasl
The surface of a stainless steel fiber was made larger, porous and cohesive by platinizing for tightly attachment of its coating. Then, it was coated by a polyaniline/polypyrrole/graphene oxide (PANI/PP/GO) nanocomposite film using electrochemical polymerization (EP) process. The prepared PANI/PP/GO fiber was used for headspace solid-phase microextraction (HS-SPME) of linear aliphatic aldehydes in rice samples followed by gas-chromatography flame ionization detector (GC-FID) determination. To achieve the highest extraction efficiency, various experimental parameters including extraction time and temperature, matrix modifier, and desorption condition were studied...
June 15, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28618051/quantitative-bioanalysis-of-bavachalcone-in-rat-plasma-by-lc-ms-ms-and-its-application-in-a-pharmacokinetics-study
#12
Dan Zhou, Lianhua An, Yan Xia, Yuanyi Wang, Xingliang Li
This study aims to develop and validate a simple and sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for investigating the pharmacokinetic characteristics of bavachalcone. Liquid-liquid extraction was used to prepare plasma sample. Chromatographic separation of bavachalcone and IS was achieved by using a Venusil ASB C18 (2.1 × 50 mm, 5 μm) column with a mobile phase of methanol (A)-water (B) (70:30, v/v). The detection and quantification of analytes was performed in selected-reaction monitoring mode using precursor→product ion combinations of m/z 323...
June 15, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28618049/study-of-gliquidone-degradation-behavior-by-high-performance-thin-layer-chromatography-and-ultra-performance-liquid-chromatography-methods
#13
Nada S Abdelwahab, Mohammed T ELsaady, Aml G Korany, Maha A Hegazy
Gliquidone (GQ) is an oral hypoglycemic agent, belongs to second generation sulfonylurea derivatives. New high-performance thin-layer chromatography (HPTLC) and ultra-performance liquid chromatography (UPLC) methods have been developed and validated and used for complete stability study of GQ following ICH guidelines. GQ was subjected to stress and forced degradation under hydrolytic, oxidative and photolytic conditions. The drug was found to be unstable under acidic, alkaline and oxidative conditions with the formation of gliquidone sulfonamide (GQS) while, a marked stability was confirmed under thermal and photolytic stress conditions...
June 15, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28617978/enantioselective-determination-of-r-manidipine-and-s-manidipine-in-human-plasma-by-a-sensitive-and-selective-chiral-lc-ms-ms-assay
#14
Vinayender Adireddy, Bhavani Prasanna Kumar Bimireddy, Venkateswarlu Ponneri
A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) assay method has been developed and validated for the enantioselective determination of manidipine in human plasma using isotope labeled compounds as internal standards. After solid phase extraction, R-(-)-manidipine and S-(+)-manidipine were chromatographed on a Chiralpack IC-3 C18 column using a isocratic mobile phase composed of 2mM ammonium bicarbonate and acetonitrile (15:85, v/v). The precursor ion to product ion transitions for the enantiomers and internal standards were monitored in the multiple reaction monitoring and positive ionization mode using a API-4000 mass spectrometer...
June 15, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28593732/severe-metabolic-impairment-with-increasing-age-for-cyp3a-and-cyp2d-substrates-in-rats-possible-consequences-for-drug-development
#15
LETTER
Nuggehally R Srinivas
No abstract text is available yet for this article.
June 7, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28558416/fast-ultra-high-performance-liquid-chromatography-with-diode-array-and-mass-spectrometry-method-for-determination-of-tadalafil-drug-substance-and-its-impurities
#16
Ludovit Schreiber, Radoslav Halko, Milan Hutta
Tadalafil is used for the treatment of erectile dysfunction and its related patents expired in 2016, therefore related generic drugs production is supposed to be increased. This work is focused on developing a fast ultra high performance liquid chromatography (UHPLC) with diode array detector (DAD) and/or mass spectrometry (MS) detection for the separation and determination of tadalafil and its impurities in pharmaceutical samples. Modern reversed-phase stationary phase with sub-2 micron particle size, Zorbax StableBond Rapid Resolution High Definition with octylsilane chemically bonded phase to totally porous silica particles was used for the solving this problem...
May 30, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28558153/quantification-of-morusin-using-lc-ms-in-rat-plasma-application-to-a-pharmacokinetic-study
#17
Zhipeng Deng, Xiaohui Sun, Shenbao Yang, Lei Zhang, Peilu Sun, Xuepeng Teng, Hao Zhong
A sensitive LC-MS method was developed for the quantification of morusin in rat plasma using praeruptorin C as internal standard. After extraction with diethyl ether, post-treatment samples were chromatographed on a Hypersil C18 column. An isocratic mobile phase consisting of methanol-water (70:30, v/v) was applied at a flow rate of 0.4 mL/min. Detection was performed via electrospray ionization source with positive ion mode using selected ion monitoring mode at m/z 443.1 for morusin and m/z 451.0 for IS. Acceptable linearity (r(2) ≥ 0...
May 30, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28557071/single-step-isolation-of-embelin-using-high-performance-countercurrent-chromatography-and-determination-of-the-fatty-acid-composition-of-seeds-of-embelia-schimperi
#18
Minaleshewa Atlabachew, Bewketu Mehari, Sandra Combrinck, Robert McCrindle
Embelin(2,5-dihydroxy-3-undecyl-p-benzoquinone)is known for its potent anthelmintic activity, but also for wound-healing, antidiabetic, anticonvulsant, antitumour, anti-inflammatory, analgesic, hepatoprotective, antioxidant, antibacterial andantispermatogenic activities. A high performance countercurrent chromatography method was developed for the purification of embelin from an extract of the seeds of Embelia schimperi fruit. The optimised solvent system (n-hexane:ethylacetate:ethanol:water (7:3:7:3)) resulted in the isolation of 13...
May 30, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28557019/development-of-a-validated-uplc-ms-ms-method-for-determination-of-humantenmine-in-rat-plasma-and-its-application-in-pharmacokinetics-and-bioavailability-studies
#19
Yanxian Hu, Ming Hao Chen, Zhaoyu Wang, Yao Lan, Lan Tang, Menghua Liu, Jie Zhao, Ming Hu, Lulu Zhang, Ling Ye
Humantenmine (HMT), the most toxic compound isolated from Gelsemium elegans Benth, is a well-known active herbal compound. A rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to estimate the absolute oral bioavailability of HMT in rats. Quantification was performed by multiple reaction monitoring using electrospray ionization (ESI) operated in positive ion mode with transitions of m/z 327.14 → m/z 296.19 for HMT and m/z 323...
May 30, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28557007/simultaneous-determination-of-ginsenoside-rb1-ginsenoside-rg1-paeoniflorin-albiflorin-and-oxypaeoniflorin-in-rat-plasma-by-liquid-chromatography-tandem-mass-spectrometry-application-to-a-pharmacokinetic-study-of-wen-yang-huo-xue-soft-capsule
#20
Xiujun Wu, Yang You, Gonglin Qu, Ran Ma, Mingxue Zhang
A simple and sensitive HPLC-MS/MS method was developed and fully validated for simultaneous determination of ginsenoside Rb1, ginsenoside Rg1, paeoniflorin, albiflorin and oxypaeoniflorin in rat plasma. Plasma samples were pretreated with protein precipitation using acetonitrile. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of acetonitrile and water (containing 0.1% formic acid). All analytes and digoxin (internal stand, IS) were quantitated through electrospray ionization in negative ion multiple reaction monitoring (MRM) mode...
May 30, 2017: Biomedical Chromatography: BMC
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