journal
MENU ▼
Read by QxMD icon Read
search

Biomedical Chromatography: BMC

journal
https://www.readbyqxmd.com/read/27891633/direct-inhibition-of-re-du-ning-injection-and-its-active-compounds-on-human-liver-cytochrome-p450-enzymes-by-a-cocktail-method
#1
Danyu Kang, Ting Geng, Yuanpei Lian, Yanjing Li, Gang Ding, Wenzhe Huang, Shiping Ma, Zhenzhong Wang, Zheng Ma, Wei Xiao
The aim of this study was to investigate the direct inhibitory effects of Re Du Ning Injection (RDN) and its active compounds on the major CYP isoforms (CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6 and CYP3A4) of human liver microsomes by "a cocktail method". The activity of each CYP isform was represented as the formation rate of the specific metabolite from relevant substrate. Then a sensitive and specific ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to simultaneously analyze the seven metabolites...
November 28, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27882562/the-development-and-validation-of-a-high-throughput-lc-ms-ms-method-for-the-analysis-of-endogenous-%C3%AE-hydroxy-%C3%AE-methylbutyrate-hmb-in-human-plasma
#2
Subramanian Ramachandran, Stefan Ehling, Sathyavageeswaran Shreeram, Todime M Reddy
A high-throughput, sensitive, and rugged liquid chromatography - tandem mass spectrometry (LC-MS/MS) method for the rapid quantitation of β-hydroxy-β-methylbutyrate (HMB) in human plasma has been developed and validated for routine use. The method uses 100 μL of plasma sample and employs protein precipitation with 0.1% formic acid in methanol for the extraction of HMB from plasma. Sample extracts were analyzed using LC-MS/MS technique under negative mode electrospray ionization conditions. A (13) C-labelled stable isotope internal standard was used to achieve accurate quantitation...
November 24, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27868218/simultaneous-determination-of-l-tetrahydropalmatine-and-its-active-metabolites-in-rat-plasma-by-a-sensitive-ultra-high-performance-liquid-chromatography-with-tandem-mass-spectrometry-method-and-its-application-in-a-pharmacokinetic-study
#3
Weihui Wang, Jing Liu, Xiaoning Zhao, Yan Peng, Nannan Wang, David Y W Lee, Ronghua Dai
A sensitive and reliable ultra high performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for simultaneous determination of L-tetrahydropalmatine (L-THP) and its active metabolites L-isocorypalmine (L-ICP) and L-corydalmine (L-CD) in rat plasma,. The analytes were extracted by liquid-liquid extraction and separated on a Bonshell ASB C18 column (2.1 mm × 100 mm; 2.7 µm; Agela) using acetonitrile-formic acid aqueous as mobile phase at a flow rate of 0...
November 21, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27864843/measurement-of-caffeine-and-its-three-primary-metabolites-in-human-plasma-by-hplc-esi-ms-ms-and-clinical-application
#4
Feng Chen, Zhe-Yi Hu, Robert B Parker, S Casey Laizure
Caffeine is a mild stimulant with significant potential for abuse being consumed in larger doses with the widespread availability of energy drinks and by novel routes of administration such as inspired powder, oral sprays, and electronic cigarettes. How these recent changes in caffeine consumption affecting caffeine disposition and abuse potential is of growing concern. In the study of caffeine disposition in humans, it is common to only measure the caffeine concentration; however, caffeine's three major metabolites (paraxanthine, theobromine, and theophylline) retain central nervous system stimulant activity that may contribute to the overall pharmacological activity and toxicity...
November 18, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27859445/quantification-of-furosemide-in-camel-plasma-by-high-resolution-mass-spectrometry-application-on-pharmacokinetics
#5
Ibrahim A Wasfi, Sayed A Wajid, B A Agha, Asmaa M Kamel, Nasreen A Al Biriki, Khaled M Al Neaimi, Waleed A Al Ali
We developed and validated a high resolution liquid chromatography mass spectrometry method for the quantification of furosemide in camel plasma which was used for a pharmacokinetic study in camels. Plasma samples were extracted by supported liquid extraction and furosemide and internal standard (furosemide-D5) were separated on a an Agilent Zorbax XDB C18 column (50 mm × 2.1 mm i.d., 3.5 µm). Data was acquired in full-scan mode over a mass range of 200-400 Da in negative electro spray mode at a resolution of 70,000...
November 18, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27859484/gram-microorganisms-dna-polymerase-inhibition-antibacterial-and-chemical-properties-of-fruit-and-leaf-extracts-of-sorbus-acuparia-and-sorbus-caucasica-var-yaltirikii
#6
Halbay Turumtay, Ahmet Midilli, Emine Akyuz Turumtay, Adem Demir, Emine Kılıçkaya Selvi, Emine Esra Budak, Havva Er, Fatih Kocaimamoglu, Hüseyin Baykal, Ali Osman Belduz, Vagif Atamov, Cemal Sandallı
Investigation of novel plant-based agents might provide alternative antibiotics and thus fight antibiotic resistance. Here, we measured the ability of fruit and leaf extracts of Sorbus aucuparia (Sauc) and endemic Sorbus caucasica var. yaltirikii (Scau) to inhibit non-replicative (Klenow Fragment-KF and Bacillus Large Fragment-BLF) and replicative (DnaE and PolC) bacterial DNA polymerases along with their antimicrobial, DPPH free radical scavenging activity (RSA), and chemical contents by total phenolic content (TPC) and HPLC-DAD analysis...
November 17, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27859443/metabolic-characterization-of-the-early-stage-of-hepatic-fibrosis-in-rat-using-gc-tof-ms-and-multivariate-data-analyses
#7
Hui Jiang, Jun-Mei Song, Peng-Fei Gao, Xiu-Juan Qin, Shuang-Zhi Xu, Jia-Fu Zhang
The aim of this study was to explore the changes in the urine metabolic spectrum in rats with the early stage of liver fibrosis using gas chromatography-time of flight/mass spectrometry (GC-TOF/MS), try to search for potential biomarkers and elucidate the probably metabonomic pathogenesis. The early stage of liver fibrosis was established with a single subcutaneously injection carbon tetrachloride (CCl4 ) twice each week for 4 weeks continuously. At the end of the experiment, GC-TOF/MS technology with multivariate statistical approaches such as principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) was used to analyze the changes in the metabolic spectrum trajectory and identify potential biomarkers...
November 17, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27859537/development-of-a-targeted-method-for-quantification-of-gypenoside-xlix-in-rat-plasma-using-spe-and-lc-ms-ms
#8
Song Guo, Chengxu Sui, Ying Ma
A sensitive, selective and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of gypenoside XLIX, a naturally occurring gypenoside of Gynostemma pentaphyllum in rat plasma and then validated according to FDA Guidance for industry-bioanalytical method validation. Plasma samples were prepared by a simple solid-phase extraction. Separation was performed on a Waters XBridgeTM BEH C18 chromatography column (4.6 mm × 50 mm, 2.5 µm) using a mobile phase of acetonitrile and water (62...
November 16, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27859496/application-of-a-solvent-free-solid-injection-technique-coupled-with-gc-ms-for-discrimination-between-the-secondary-metabolites-of-wild-and-cultivated-south-korean-medicinal-foods
#9
Lieu T B Truong, A M Abd El-Aty, Hyun Jin Kim, Md Musfiqur Rahman, Sung Woo Kim, Ho-Chul Shin, Jae Han Shim
Solvent-free solid injection was applied to differentiate between wild and cultivated South Korean medicinal foods, including dureup (Aralia elata), deodeok (Codonopsis lanceolata), and doraji (Platycodon grandiflorus). A number of compounds were identified in wild and cultivated dureup (53 and 46), deodeok (47 and 51), and doraji (43 and 38). Secondary metabolites, including butanal,2-methyl-, β-caryophyllene, neoclovene, α-humulene, γ-curcumene, β-bisabolene, and phytol, were identified in dureup with significantly (P < 0...
November 15, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27859454/rapid-analysis-of-interaction-between-six-drugs-and-%C3%AE-2-adrenergic-receptor-by-injection-amount-dependent-method
#10
Kaizhu Zeng, Jing Wang, Zhenyu Sun, Qian Li, Sha Liao, Xinfeng Zhao, Xiaohui Zheng
Drug-protein interaction analysis has become a considerable topic in life science which includes clarifying protein functions, explaining drug action mechanisms and uncovering novel drug candidates. This work was to determine the association constants (KA ) of six drugs to β2 -adrenergic receptor by injection amount dependent method using stationary phase containing the immobilized receptor. The association constants (KA ) were calculated to be (25.85 ± 0.035) × 10(4)  M(-1) for clorprenaline, (42...
November 15, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27859436/tissue-distribution-of-capilliposide-b-capilliposide-c-and-their-bioactive-metabolite-in-mice-using-liquid-chromatography-tandem-mass-spectrometry
#11
Zhongzhe Cheng, Xing Zhou, Bingying Hu, Wenyi Li, Guiying Chen, Yang Zhang, Jingkui Tian, Lin Zhang, Ming Li, Hongliang Jiang
Lysimachia capillipes Hemsl (Primulaceae), a folk medicinal plant in China, showed significant anti-tumor activities in vivo and vitro. Capilliposide B (LC-B) and Capilliposide C (LC-C) were the main bioactive components in this plant. To explore their tissue distribution, a reliable bioanalytical method for the quantification of LC-B, LC-C, and their bioactive metabolite, Capilliposide A (LC-A) in mice tissues was developed and validated. In this study, the tissue distribution profiles of the three compounds were examined after intravenous administration of pure LC-B and oral administration of total saponins of L...
November 14, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27862110/validated-lc-ms-ms-method-for-simultaneous-determination-of-doxorubicin-and-curcumin-in-polymeric-micelles-in-subcellular-compartments-of-mcf-7-adr-cells-by-protein-precipitation-ultrasonic-breaking-method
#12
Jinling Wang, Ying Li, Wenzhuan Ma, Xiaohui Wang, Pengfei Tu
A specific and sensitive LC-MS/MS with protein precipitation- ultrasonic breaking method has been developed and validated for simultaneously determination of doxorubicin (DOX) and curcumin (Cur) in DOX and Cur co-loaded hyaluronic acid- vitamin E succinate (HA-VES) micelles ((DOX + Cur)-PMs) in subcellular compartments of resistant MCF-7/Adr cells. Sequential extraction of four subcellular protein fractions (cytosolic, membrane/organelle, nucleic and cytoskeleton) was performed directly from MCF-7/Adr cells after incubation with (DOX + Cur)-PMs...
November 11, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27862109/simple-extraction-method-for-quantification-of-phenothiazine-residues-in-pork-muscle-using-liquid-chromatography-triple-quadrupole-tandem-mass-spectrometry
#13
Dan Zhang, Jin-A Park, Seong-Kwan Kim, Sang-Hyun Cho, Soo-Min Cho, Jae-Han Shim, Jin-Suk Kim, A M Abd El-Aty, Ho-Chul Shin
In this study, an analytical method was developed for quantification of residues of the anthelmintic drug phenothiazine (PTZ) in pork muscle using liquid chromatography-tandem mass spectrometry. Muscles were extracted using 0.2% formic acid and 10 mM ammonium formate in acetonitrile, defatted, and purified using n-hexane. The drug was well separated on a Waters XBridge™ C18 analytical column using a binary solvent system consisting of 0.2% formic acid and 10 mM ammonium formate in ultrapure water (A) and acetonitrile (B)...
November 10, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27862090/chromatographic-determination-of-some-biomarkers-of-liver-cirrhosis-and-hepatocellular-carcinoma-in-egyptian-patients
#14
Diaa Osman, Omnia Ali, Manar Obada, Hatem El-Mezayen, Hala El-Said
Metabolomics has been shown to be an effective tool for disease diagnosis, biomarker screening and characterization of biological pathways. A total of 140 subjects were included in this study; urine metabolomes of patients with liver cirrhosis (LC, n = 40), patients with HCC (n = 55) and healthy male subjects (n = 45); as control group, were studied. Gas chromatography/mass spectrometry based urine metabolomics profile was investigated for all participants. Diagnostic models were constructed with a combination of marker metabolites, using principal components analysis (PCA) and receiver operator characteristic curves...
November 10, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27859431/development-of-a-facile-and-sensitive-hplc-fld-method-via-fluorescence-labeling-for-triterpenic-acid-bioavailability-investigation
#15
Jinmao You, Di Wu, Mei Zhao, Guoliang Li, Peiwei Gong, Yueyue Wu, Yu Guo, Guang Chen, Xianen Zhao, Zhiwei Sun, Lian Xia, Yongning Wu
Triterpenic acids are widely distributed in many fruits and are known for their medicinal benefits. The study of bioavailability has been an important task for a better understanding of the triterpenic acids. Although many methods based on fluorescence labeling for triterpenic acid determination have been established, these reported methods needed anhydrous conditions, which are not suitable for the convenient study of triterpenic acid bioavailability. Inspired by that, a versatile method, which overcomes the difficulty of the reported methods, has been first developed in this study...
November 10, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27862112/qualitative-and-quantitative-analysis-of-major-constituents-from-dazhu-hongjingtian-capsule-by-uplc-q-tof-ms-ms-combined-with-uplc-qqq-ms-ms
#16
Guang-Da Liu, Yi-Wu Zhao, Yan-Jing Li, Xue-Jing Wang, Hai-Hong Si, Wen-Zhe Huang, Zhen-Zhong Wang, Shi-Ping Ma, Wei Xiao
In this work, a sensitive and efficient method was established and validated for qualitative and quantitative analysis of major bioactive constituents in Dazhu Hongjingtian capsule by liquid chromatography tandem mass spectrometry (LC-MS). A total of 32 compounds were tentatively identified using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS/MS). Furthermore, twelve constituents, namely gallic acid, 3,4-dihydroxybenzoic acid, salidroside, p-coumaric acid-4-O-β-D-glucopyranoside, bergeninum, 4-hydroxybenzoic acid, 4-hydroxyphenylacetic acid, syringate, 6''-O-galloylsalidroside, rhodiosin, rhodionin and kaempferol-7-O-α-L-rhamnoside, were simultaneously quantified by the developed ultra-performance liquid chromatography coupled with a triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS) method in 9 min...
November 9, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27859468/biomedical-chromatography-2017
#17
EDITORIAL
Michael G Bartlett
No abstract text is available yet for this article.
November 9, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27862094/uplc-ms-ms-method-for-the-determination-of-tobacco-specific-biomarker-nnal-tamoxifen-and-its-main-metabolites-in-rat-plasma
#18
Wei Xiong, Jiajia Zhao, Lin Wang, Xuehua Jiang
Cigarette smoke is known to interact with tamoxifen metabolizing enzymes and transporters and potentially affect its treatment outcome. 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanol (NNAL) is an important metabolite of 4-(methylnitro-samino)-1-(3-pyridyl)-1-butanone (NNK) because it is frequently used as biomarker to assess human smoke exposure. In order to study the potential pharmacokinetic interaction between cigarette smoke and tamoxifen in rats. A UPLC-MS/MS method for the simultaneous determination of NNAL, tamoxifen along with its metabolites in rat plasma has been developed and validated...
November 8, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27809345/bioequivalence-study-between-a-fixed-dose-single-pill-formulation-of-nebivolol-plus-hydrochlorothiazide-and-separate-formulations-in-healthy-subjects-using-high-performance-liquid-chromatography-coupled-to-tandem-mass-spectrometry
#19
Celso Francisco Pimentel Vespasiano, Tiago Luders Laurito, Renan Donomae Iwamoto, Ronilson Agnaldo Moreno, Gustavo D Mendes, Gilberto De Nucci
Systemic arterial hypertension is a major risk factor for cerebrovascular disease. Therefore, an adequate control of blood pressure is of enormous importance. One of the many fixed-dose single-pill antihypertensive formulations available on the market is the combination of nebivolol and hydrochlorothiazide. The objective of this study was to develop two distinct high performance liquid chromatography coupled to tandem mass spectrometry methods to simultaneously quantify nebivolol and hydrochlorothiazide in human plasma...
November 3, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27809343/validated-high-performance-liquid-chromatography-method-for-the-determination-of-the-inhibitor-of-cancer-cell-invasion-e-n-benzyl-6-2-3-4-dihydroxy-benzylidene-hydrazinyl-n-methylpyridine-3-sulfonamide-in-rat-plasma-and-its-application-to-pharmacokinetic-study
#20
Tae Kon Kim, Hyun Kyung Choi
In this study, a reliable method for the quantitation of (E)-N-benzyl-6-(2-(3, 4-dihydroxy benzylidene)hydrazinyl)-N-methylpyridine-3-sulfonamide (JW-55) in rat plasma was developed and validated using high-performance liquid chromatography (HPLC). Plasma samples were deproteinized; sildenafil was used as an internal standard. Chromatographic separation was achieved using a reversed-phase (C18 ) column. The mobile phase, 0.02 M ammonium acetate buffer:acetonitrile (48:52, v/v), was run at a flow rate of 1...
November 3, 2016: Biomedical Chromatography: BMC
journal
journal
29346
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"