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Biomedical Chromatography: BMC

Jun Qian, Fan Xie, Yanhong Shi, Jinhang Li, Liuqiang Zhang, Yiming Li, Fujiang Guo, Rui Wang
Bavachinin (BVC), one of the main bioactive prenylated flavonoids derived from Psoralea corylifolia Linn, has a wide variety of pharmacological effects, such as antiangiogenic, antitumour, antiallergic, anti-inflammatory, and antibacterial activities, especially as a pan-peroxisome proliferator-activated receptors (PPARs) agonist. A rapid and sensitive method for quantifying BVC in rat plasma was developed and validated through ultra-high-performance liquid chromatography coupled with electrospray-ionization tandem mass spectrometry...
May 21, 2018: Biomedical Chromatography: BMC
Aiyi Xin, Yaming Zhang, Yanxia Zhang, Duolong Di, Junxi Liu
For the rational utilization and the quantitative quality control of the Stephania yunnanensis Lo, an HPLC-DAD method was developed for the quantitative and simultaneous determination of five alkaloids in rat plasma (stepharine, sinomenine, palmatine, isocorydine and tetrahydropalmatine), which were the main active chemical constituents of this plant and belong to four kinds of isoquinoline-type alkaloids (protoberberine, morphine, aporphine and protaporphine alkaloids). The contents of five alkaloids ranged from 0...
May 21, 2018: Biomedical Chromatography: BMC
Abhaysingh Bhadoriya, Mallika Sanyal, Priyanka A Shah, Pranav S Shrivastav
A simple, high-throughput and highly sensitive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous estimation of rosuvastatin and free ezetimibe. Liquid-liquid extraction was carried out using methyl-tert butyl ether after prior acidification from 300 μL human plasma. The recovery for both the analytes and their deuterated internal standards (ISs) ranged from 95.7-99.8 %. Rosuvastatin and ezetimibe were separated on Symmetry C18 column using acetonitrile and ammonium formate buffer, pH 3...
May 21, 2018: Biomedical Chromatography: BMC
Wei-Qi Xie, Yi-Xian Gong, Kong-Xian Yu
A reaction headspace gas chromatography (HS-GC) technique was investigated for quantitatively analyzing trichloroacetic acid in human urine. This method is on the basis of the decomposition reaction of trichloroacetic acid under high temperature conditions. The carbon dioxide and chloroform formed from the decomposition reaction can be respectively detected by the thermal conductivity detector HS-GC and the flame ionization detector HS-GC. The data showed that the reaction can be completed in 60 min at 90 o C...
May 16, 2018: Biomedical Chromatography: BMC
Hyuga Moriya, Sohei Tanaka, Yukari Iida, Satomi Kitagawa, Sen-Ichi Aizawa, Atsushi Taga, Hiroyuki Terashima, Atsushi Yamamoto, Shuji Kodama
Xanthohumol, isoxanthohumol, and 8-prenylnaringenin in beer, hop, and hop pellet samples were analyzed by HPLC using InertSustain phenyl column and the mobile phase containing 40% methanol and 12% 2-propanol. Fractions of isoxanthohumol and 8-prenylnaringenin obtained by the above HPLC were separately collected. Isoxanthohumol and 8-prenylnaringenin were enantioseparated by HPLC using Chiralcel OD-H column with a mobile phase composed of hexane/ethanol (90/10, v/v) and Chiralpak AD-RH column with a mobile phase composed of methanol/2-propanol/water (40/20/40, v/v/v), respectively...
May 16, 2018: Biomedical Chromatography: BMC
Robert B Flint, Soma Bahmany, Bart C H van der Nagel, Birgit C P Koch
A simple and specific UPLC-MS/MS method was developed and validated for simultaneous quantification of fentanyl, sufentanil, cefazolin, doxapram and its active metabolite keto-doxapram. The internal standard was fentanyl-d5 for all analytes. Chromatographic separation was achieved with a reversed phase Acquity UPLC HSS T3 column with a run-time of only 5.0 minutes per injected sample. Gradient elution was performed with a mobile phase consisting of ammonium acetate, formic acid in Milli-Q ultrapure water or in methanol with a total flow rate of 0...
May 16, 2018: Biomedical Chromatography: BMC
Naoual Dahmana, Doris Gabriel, Robert Gurny, Yogeshvar N Kalia
Glucocorticoids are a mainstay for the treatment of immune-mediated conditions and inflammatory diseases. However, their chronic use causes numerous side-effects including delays in corneal and cutaneous wound healing. This is attributed to off-target agonism of the mineralocorticoid receptor, which can be reduced by co-administration of a mineralocorticoid receptor antagonist such as spironolactone. The aim of this study was to develop a fast, selective and sensitive UHPLC-ESI-MS method for the simultaneous quantification of spironolactone, its active metabolites - 7α-thiomethylspironolactone and canrenone, the latter's water-soluble prodrug potassium canrenoate, and the synthetic glucocorticoid, dexamethasone, in corneal samples (17α-methyltestosterone served as an internal standard)...
May 16, 2018: Biomedical Chromatography: BMC
Peter Oertel, Andreas Bergmann, Sina Fischer, Phillip Trefz, Anne Küntzel, Petra Reinhold, Heike Köhler, Jochen K Schubert, Wolfram Miekisch
Volatile organic compounds (VOCs) emitted from in vitro cultures may reveal information on species and metabolism. Due to low nmol L-1 concentration ranges, pre-concentration techniques are required for gas-chromatography mass-spectrometry (GC-MS) based analyses. This study was intended to compare the efficiency of established micro-extraction techniques - solid-phase micro-extraction (SPME) and needle-trap micro-extraction (NTME) - for the analysis of complex VOC patterns. For SPME, a 75 μm Carboxen®/Polydimethylsiloxane fibre was used...
May 14, 2018: Biomedical Chromatography: BMC
Lin Chen, Yuetao Liu, Qingfeng Guo, Qingxia Zheng, Wancun Zhang
In this study, a systematic research on the metabolome differences between wild Ophiocordyceps sinensis (O. sinensis) and artificial cultured Cordyceps militaris (C. militaris) was conducted using liquid chromatography-mass spectrometry. Principal components analysis (PCA) and Orthogonal projection on latent structure-discriminant analysis (OPLS-DA) results showed that C. militaris grown on solid rice medium (R-CM) and C. militaris grown on tussah pupa (T-CM) evidently separated and individually separated from wild O...
May 11, 2018: Biomedical Chromatography: BMC
Giuseppe Corona, Milena Gusella, Anna Gaspardo, Gianmaria Miolo, Laura Bertolaso, Elisa Pezzolo, Felice Pasini, Agostino Steffan, Donatella Caruso
A rapid and sensitive LC-MS/MS method for therapeutic drug monitoring oral vinorelbine (VRL) metronomic anticancer chemotherapy has been developed and validated. Analysis of VRL and its main active metabolite 4-O-deacetylvinorelbine (M1) was performed in whole blood matrix. Both analytes were extracted by protein precipitation and separated on an Onyx monolith C18, 50 x 2 mm column then quantified by positive electrospray ionization and multiple reaction monitoring mode. The LLOQ was 0.05 ng/mL for both VRL and M1...
May 10, 2018: Biomedical Chromatography: BMC
Na Feng, Yutian Wei, Jie Feng, Qingjiu Tang, Zhong Zhang, Jingsong Zhang, Han Wei
Ganoderic acid S, ganoderic acid T, and ganoderal B are the main bioactive triterpenes of Ganoderma lucidum. In this study, mycelia of Ganoderma lucidum were obtained by two-stage fermentation and then extracted by ethanol and petroleum ether sequentially to obtain crude triterpenes. The crude sample was further purified by recycling high-speed counter-current chromatography (HSCCC) with n-hexane: ethyl acetate: methanol: water (7:12:11:5, v/v/v/v) as the optimized two-phase solventsystem. 16.4 mg of ganoderol B with the purity of 90...
May 10, 2018: Biomedical Chromatography: BMC
Lei Zhang, Fei Li, Wang-Jun Qin, Chao Fu, Xiang-Lin Zhang
Ginsenoside Re, an active ingredient in Panax ginseng, is widely used as a therapeutic and nutriment. Intestinal microbiota plays crucial roles in modulating the pharmacokinetics and pharmacological actions of ginsenoside Re. The aim of this study was to explore the relationship between bacterial community variety and the metabolic profiles of ginsenoside Re. We developed two models with intestinal dysbacteriosis: a pseudo-germ-free model induced by a non-absorbable antimicrobial mixture (ATM), and Qi-deficiency model established via over-fatigue and acute cold stress (OACS)...
May 10, 2018: Biomedical Chromatography: BMC
Jagdish Mahale, Lynne M Howells, Rajinder Singh, Robert G Britton, Hong Cai, Karen Brown
Curcumin, derived from turmeric, has been extensively investigated for its broad spectrum of biological activities. Previously reported HPLC-UV methods have focussed on analysis of the parent compound. Here, a sensitive HPLC-UV method was developed and partially validated, then used for the simultaneous determination of curcumin and its glucuronide and sulfate metabolites in plasma and lung tissue from mice. The assay was applied to an in vivo pharmacokinetic study comparing formulated curcumin (Meriva™) with standard curcumin...
May 9, 2018: Biomedical Chromatography: BMC
Peng-Cheng Guo, Hua-Dan Shen, Jiang-Ji Fang, Tian-Ming Ding, Xiao-Ping Ding, Jun-Feng Liu
An on-line high performance liquid chromatography- biochemical detection (HPLC-BCD) method, which compounds separated by HPLC were on-line reacted with enzyme and substrate solutions delivered by flow injection and enzyme inhibition signal was collected in time by UV detector, was developed to rapidly screen α-glucosidase inhibitors from green tea extracts in this study. The chromatographic fingerprints and enzyme inhibition profiles of the different brands of green tea could be simultaneously detected by the on-line HPLC-BCD method...
May 9, 2018: Biomedical Chromatography: BMC
Ying Xia, Shunbo Zhao, Meng Gong, Li Ding
A rapid, sensitive and reproducible LC-MS/MS method was developed and validated to determine iguratimod in human plasma. Sample preparation was achieved by protein precipitation with acetonitrile. Chromatographic separation was operated on an Ultimate® XB-C18 column (2.1 × 50 mm, 3.5 μm, Welch) with a flow rate of 0.400 mL/min, using a gradient elution with acetonitrile and water which contained 2 mM ammonium acetate and 0.1% formic acid as the mobile phase. The detection was performed on a Triple QuadTM 5500 mass spectrometer coupled with electrospray ionization interface under positive-ion multiple reaction monitoring mode with the transition ion pairs of m/z 375...
May 5, 2018: Biomedical Chromatography: BMC
Marta Karaźniewicz-Łada, Anna Główka, Anna Komosa, Łukasz Kruszyna, Agnieszka Graczyk-Szuster, Maciej Lesiak, Dagmara Sowińska, Juliusz Przysławski
Fat-soluble vitamins play a pivotal role in the progression of atherosclerosis and development of cardiovascular disease. Therefore, plasma monitoring of their concentrations may be useful in the diagnosis of these disorders as well as in the process of treatment. The study aimed to develop and validate an HPLC-MS/MS method for determination of retinol, α-tocopherol, 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in plasma of patients with cardiovascular disease. The analytes were separated on HPLC Kinetex F5 column via gradient elution with water and methanol, both containing 0...
May 5, 2018: Biomedical Chromatography: BMC
Rong-Xia Liu, You-Yan Xian, Sha Liu, Fei Yu, Hong-Jie Mu, Kao-Xiang Sun, Wan-Hui Liu
No abstract text is available yet for this article.
May 4, 2018: Biomedical Chromatography: BMC
Jiang Wang, Naresh Pavurala, Xiaoming Xu, Yellela S R Krishnaiah, Patrick J Faustino
To evaluate the bioavailability and pharmacokinetic profiles of two novel galantamine formulations as medical countermeasure products, an ultra-performance liquid chromatography-single quadrupole mass spectrometry (UPLC-MS) method was developed and validated for quantifying galantamine in guinea pig plasma using solid phase extraction with a mixed mode strong cation exchange reversed phase cartridge. Chromatographic separation was achieved on a Waters Acquity UPLC BEH C18 column maintained at 40 °C. The mobile phases were solution-A acetonitrile-water (5:95, v/v) and solution-B acetonitrile-water (90:10, v/v), which both contain 2 mM ammonium formate and 0...
May 4, 2018: Biomedical Chromatography: BMC
Massimo Tempestilli, Adriana Ammassari, Antonio D'Avolio, Stefania Cicalini, Anna Loredana Gallo, Simone Fazio, Andrea Antinori, Leopoldo P Pucillo
Therapeutic drug monitoring may be crucial in selected clinical conditions for the management of HIV infection. In the last years, new antiretrovirals have been introduced and in particular elvitegravir (EVG) is now recommended for first-line and in simplification treatment as well as dolutegravir (DTG) and rilpivirine (RPV). The aim of this study was to develop and validate a High Performance Liquid Chromatography-Ultra Violet (HPLC-UV) method for determining EVG and others newest antiretrovirals DTG and RPV in human plasma...
May 4, 2018: Biomedical Chromatography: BMC
Peiwu Geng, Jun Luo, Ziwei Weng, Zhenhua Fan, Bing Zhang, Jianshe Ma, Xianqin Wang, Meiling Zhang
The purpose of this study is to develop an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method to determine armepavine in mouse blood. Nuciferine was used as internal standard (IS). Chromatographic separation was performed on a UPLC BEH (2.1 mm×50 mm, 1.7 μm) column with a gradient elution of acetonitrile and 10 mmol/L ammonium acetate solution (containing 0.1% formic acid). The quantitative analysis was conducted in MRM model with m/z 314.1→106.9 for armepavine and m/z 296...
May 3, 2018: Biomedical Chromatography: BMC
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