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Biomedical Chromatography: BMC

Wenzheng Ding, Hanxue Wang, Quan Zhou, Jinrong Wu, Xuemei Cheng, Changhong Wang
Gentiana macrophylla Radix (GMR) is officially used as traditional Chinese medicine, but easily confused with Gentianae radix et rhizoma (GRR) and adulterants. The paper aims to establish a HPLC method for qualitative and quantitative analysis of GMR based on characteristic components, anofinic acid and its derivatives. HPLC analysis was performed on a C18 column with gradient elution using acetonitrile and 0.1% phosphoric acid as mobile phase, and detected at 240 nm, by conventional methodology validation...
July 14, 2018: Biomedical Chromatography: BMC
Neeraj Kumar Saini, Suresh P Sulochana, Vinay Kiran, Mohd Zainuddin, Ramesh Mullangi
A simple, sensitive and rapid assay method has been developed and validated for the estimation of apalutamide on mice dried blood spots (DBS) using liquid chromatography coupled to tandem mass spectrometry with electro spray ionization in the positive-ion mode. The method utilizes liquid extraction of apalutamide from 3 mm punched disks from DBS cards (spiked or study samples). The extracted sample was chromatographed using a gradient on an Atlantis dC18 column using gradient elution with 0.2% formic acid and acetonitrile at a flow rate of 1...
July 14, 2018: Biomedical Chromatography: BMC
Gabriel Hancu, Monica Budău, Daniela Lucia Muntean, Laszlo Gagyi, Aura Rusu
Chirality is a primordial subject in modern drug research as well as in pharmaceutical industry and development. Almost all second-generation modern antidepressants are chiral substances; however in therapy some are used as racemic mixtures while others as pure enantiomers. The development of enantioseparation methods of chiral antidepressants and their metabolites is one of the keys towards understanding their enantioselective drug action. For this purpose, efficient and reliable analytical methods are needed, and capillary electrophoresis proved to be an interesting and advantageous alternative to the more frequently used chromatographic techniques...
July 13, 2018: Biomedical Chromatography: BMC
Ying Li, Lifang Wang, Xianzhao Yang, Yongan Ye, Pengfei Tu, Jinling Wang
Tubeimoside I (Tub), a triterpenoid saponin isolated from R. Bolbostematts. A sensitive and validated method was developed to determine Tub in rat plasma. This method combined the qualitative and quantitative advantages from a liquid chromatography coupled with hybrid ion trap-time of flight mass spectrometer (HPLC-DAD-IT-TOF-MS) and a triple quadrupole-linear ion trap mass spectrometer 5500 (Qtrap 5500), due to the narrow molecular range of Qtrap 5500 relative to the molecular weight of Tub. Initially, ion detection was achieved using negative ionization mode along with full scan on IT-TOF-MS...
July 13, 2018: Biomedical Chromatography: BMC
Jian Zheng, Changmin Shao, Bo Fan, Lijia Jing, Siyang Li, Xiufeng Yan, Yang Wang
In the present study, a 10-position modified of camptothecin, 10-propionyloxy camptothecin (PCPT) is esterified from 10-hydroxcamptothecin (HCPT), which could metabolize to HCPT in vivo. PCPT displayed a relatively stronger antitumor activity in vitro and in vivo. Thereafter a simple, sensitive and rapid HPLC method coupled with a fluorescence detector has been developed and validated for the assay of PCPT and its active metabolite HCPT in rat plasma. The method was validated for accuracy, precision, linearity, selectivity and recovery...
July 13, 2018: Biomedical Chromatography: BMC
Dan Huang, Qing-Song Jiang, Jun-Qing Yang, Ting Cui, Nian-Ru Wang, Ting-Ting Du, Xin-Hui Jiang
The determination of amino acids and monoamine with the actions-like neurotransmitters or modulators have been increasingly important for study the relationship between dysfunction of neurotransmitters and pathogenesis of diabetic encephalopathy. Here, a high performance liquid chromatography with fluorescence detection method was developed to simultaneously determine nine monoamines and amino acids including 3 excitatory neurotransmitters (aspartate, glutamate, and serotonin), 4 inhibitory neurotransmitters (glycine, γ-aminobutyric acid, taurine, dopamine), a precursor of 5-HT (tryptophan) and methionine using Homoserine as the internal standard...
July 13, 2018: Biomedical Chromatography: BMC
Jun Huang, Lei Yin, Lin Dong, Hongfeng Quan, Rong Chen, Shiyao Hua, Jiahua Ma, Dongyan Guo, Xueyan Fu
Radix astragali (RA) is one of the most widely used Chinese herbs prescribed in many Chinese formulas to reinforce 'Qi' and treat vital energy deficiency. This study combined fingerprint with quantitative analysis multi-components by a single marker (QAMS) to improve the quality control standard for RA on the basis of existing quality control methods of traditional Chinese medicinal materials. UPLC-ESI-TOF-MS technique was used to evaluate the quality of RA by fingerprint and QAMS. Through the anti-inflammatory, anti-oxidation and anti-anoxic activities to screen characteristic components of RA, the CG, ononin, astragaloside IV, astragaloside II, calycosin and astrageloside I significantly inhibited ear edema in mice, the calycosin and CG had good antioxidant activity, the astragaloside I had a significant anti-hypoxia activity...
July 12, 2018: Biomedical Chromatography: BMC
Hardik Chandasana, Johannes Kast, Janina A Bittman, Hartmut Derendorf
Armodafinil is a wake-promoting agent approved in 2007 by the US Food and Drug Administration for the treatment of excessive sleepiness. A rapid, sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of armodafinil in human plasma was developed and validated. Armodafinil and internal standard (armodafinil d-10) were extracted from human plasma using protein precipitation combined with liquid-liquid extraction. This developed method only requires 50 μL of plasma for the analysis...
July 12, 2018: Biomedical Chromatography: BMC
Weijia Zheng, Jeong-Min Choi, Seong-Kwan Kim, Jae-Han Shim, Young-Sun Kang, A M Abd El-Aty, Ahmet Hacımüftüoğlu, Ho-Chul Shin
A reliable and highly sensitive detection method based on liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry (LC-MS/MS) analysis has been developed for determination and quantification of halquinol, including 5,7-dichloroquinolin-8-ol and 5-chloroquinolin-8-ol. The target analytes were extracted from porcine muscle, egg, milk, eel, flatfish, and shrimp using a mixture of acetonitrile and ethyl acetate followed by liquid-liquid purification with n-hexane. The analytes were separated on an Agilent Eclipse XDB-C18 reversed-phase analytical column using 0...
July 12, 2018: Biomedical Chromatography: BMC
Marta de Diego, Diana Correa, Sigrid Mennickent, Ricardo Godoy, Carola Vergara
Vortioxetine hydrobromide (VOR), is a novel antidepressant used for the treatment of major depressive disorder. It has a chemical structure susceptible to degradation, therefore, it is important to have suitable analytical methods to determine VOR in presence of its main degradation products (DP), because if the compound degrades, this could result in diminution of the therapeutic activity and safety. A simple HPLC method with photodiode array detection (DAD), was developed and validated for determination of VOR in bulk and tablets, in presence of its major DP...
July 12, 2018: Biomedical Chromatography: BMC
Gonglin Qu, Xuesong Qi, Shuai Shao, Chen Li, Chunyan Wang, Chengfang Wang, Qiao Gou
Sijunzi decoction (SJZD) is one of the most well-known traditional Chinese herbal formulations. This study elucidates the pharmacokinetics of SJZD in rats' plasma after the administration of a single oral dose of 3 mL/kg using ultra high-performance liquid chromatography electrospray ionization quadrupole-time of flight mass spectrometry (UHPLC-ESI-Q-TOF/MS) with bergapten as an internal standard. The separation was performed on an Agilent Zorbax Eclipse Plus C18 Column (2.1 × 50 mm, 1.8 μm) by elution with acetonitrile and water (containing 0...
July 11, 2018: Biomedical Chromatography: BMC
Chu-Qin Yu, Jian-Ping Chen, Yan-Mei Zhong, Xu-Long Zhong, Chun-Ping Tang, Yi Yang, Hua-Qing Lin
Hao Jia Xu Re Qing Granules (HJ), is an effective clinically used antipyretic based on Traditional Chinese Medicine (TCM). Although its antipyretic therapeutic effectiveness is obvious, its therapeutic mechanism has not been comprehensively explored yet. In this research, we first identified potential biomarkers which may be relevant for the antipyretic effect of HJ based on urine metabolomics using Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). A rat model of fever was established using the yeast-induced febrile response...
July 7, 2018: Biomedical Chromatography: BMC
Giulio Nannetti, Silvana Pagni, Giorgio Palù, Arianna Loregian
Isavuconazole is a broad-spectrum triazole antifungal drug recently approved for the therapy of both invasive aspergillosis and mucormycosis. To support a widespread therapeutic drug monitoring (TDM) of isavuconazole, a simple, sensitive, and precise high-performance liquid chromatography (HPLC) method with UV detection was developed and fully validated for the quantification of this drug in human plasma. The method involved a combined protein precipitation -solid-phase extraction and a chromatographic separation on a Waters XTerra RP18 (150 mm × 4...
July 6, 2018: Biomedical Chromatography: BMC
Xiqian Zhang, Ruina Li, Wenya Hu, Jin Zeng, Xuehua Jiang, Ling Wang
A rapid, specific, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated to simultaneously quantify NAPQI, acetaminophen-glut, and acetaminophen-gluc in mouse plasma, liver, and kidney homogenates. Analytes were eluted by a binary gradient mobile phase composed of water (phase A) and methanol containing 0.1% formic acid (phase B) at a flow rate of 0.3 mL/min, which was performed on a CAPCELL PAK C18 MG II column. It took 3.2 min to detect three analytes in a single run...
July 5, 2018: Biomedical Chromatography: BMC
Ying Xiao, Piera Maiolino, Jin-Hua Yan, Jie Ma, Jin Li, Li Chen
In this study, a simple and sensitive LC-MS/MS method was developed and validated for simultaneous determination of icotinib and its four circulating metabolites in human plasma. The analytes were extracted with acetonitrile and separated on a C18 column using 2 mM ammonium acetate containing 0.2% formic acid and acetonitrile as mobile phase. The analytes were introduced into mass spectrometer via electrospray ionization source operated in positive ion mode. Precursor-to-product transitions were optimized to be m/z 392...
July 5, 2018: Biomedical Chromatography: BMC
Zhiyong Chen, Xia Du, Yuanyuan Yang, Xiaomin Cui, Zijia Zhang, Ye Li
Morus alba L has long been used as fodder and as a traditional medicine. Various parts of M. alba (Cortex mori, Ramulus mori, Folium mori and Fructus mori) have various bioactivities, however, most current evidence focused on anti-diabetic properties. In spite of their wide use, few studies compared the chemical composition and active components against α-glucosidase of the various medicinal parts of M. alba. In this study, we developed an HPLC method for simultaneous quality control and discrimination of Cortex mori, Ramulus mori, Folium mori and Fructus mori using thirteen marker compounds...
July 5, 2018: Biomedical Chromatography: BMC
Supaluk Krittanai, Tharita Kitisripanya, Orapin Udomsin, Hiroyuki Tanaka, Seiichi Sakamoto, Thaweesak Juengwatanatrakul, Waraporn Putalun
Pueraria candollei or White Kwao Krua (Leguminosae) is indigenous plant in Thailand which has long been used in Thai traditional medicine. The tuberous root of this plant is widely used for rejuvenation particularly in elder women. Among bioactive compounds in P. candollei, miroestrol and puerarin exhibit estrogenic activity. This study aims to develop an immunochromatographic strip (ICS) with a colloidal gold-based detection system for the simultaneous detection of miroestrol and puerarin in a one-step analysis...
July 4, 2018: Biomedical Chromatography: BMC
Guoliang Dai, Bingting Sun, Lei Wu, Xiaojun Gao, Shanshan Song, Hong Sun, Wenzheng Ju
Acute hepatitis is a severe inflammatory disease with high mortality rates. Dehydrocavidine (DC), berberine (BB) and palmatine (PT), the bioactive components of Yanhuanglian total alkaloids extract (YTAE), may play important roles in the protective effect on acute hepatitis. The present study was designed to comparative pharmacokinetic properties of DC, BB and PT after oral administration of YTAE in normal and acute hepatitis rats. The plasma pharmacokinetic profiles of three major bioactive alkaloids from YTAE were analyzed using an LC-MS/MS method...
July 4, 2018: Biomedical Chromatography: BMC
Qiaodi Gui, Jie Zheng
A method employing ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for determination of eight components including ferulic acid, senkyunolide A, butylphthalide, ligustilide, butylidenephalide, senkyunolide I, senkyunolide H and levistolide A in Angelica sinensis was established. The separation was carried out using a Waters ACQUITY UHPLC BEH C18 column with gradient elution with 0.1% formic acid aqueous and acetonitrile at a flow rate of 0.4 mL/min. Good linearity was attained with R2 of 0...
June 29, 2018: Biomedical Chromatography: BMC
Yang Liu, Ling Song, Xueting Yao, Yiwen Wu, Hongzhong Liu, Qian Zhao, Ji Jiang, Chongtie Shi, Xifeng Ma, Huimin Zhou, Dongyang Liu, Pei Hu
Imigliptin is a novel DPP-4 inhibitor, designed to treat type 2 diabetes mellitus (T2DM). A selective and sensitive method was developed using high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) to simultaneously quantify imigliptin, its five metabolites, and alogliptin in human plasma and urine. Solid-phase extraction (SPE) and direct dilution were used to extract imigliptin, its five metabolites, alogliptin from plasma and urine, respectively. The extracts were injected onto a SymmetryShield RP8 column with a gradient elution of methanol and water containing 10 mM ammonium formate (pH=7)...
June 27, 2018: Biomedical Chromatography: BMC
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