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Biomedical Chromatography: BMC

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https://www.readbyqxmd.com/read/28731579/residual-dynamic-and-risk-assessment-of-dimethomorph-in-swiss-chard-grown-in-two-different-sites
#1
Md Humayun Kabir, A M Abd El-Aty, Md Musfiqur Rahman, Hyung Suk Chung, Han Sol Lee, Mi-Ra Kim, Byung-Joon Chang, Jing Wang, Ho-Chul Shin, Jae-Han Shim
Residue analysis of dimethomorph in Swiss chard cultivated at two different locations under greenhouse conditions was conducted using high-performance liquid chromatography-ultraviolet detector (HPLC-UVD) and confirmed by tandem mass spectrometry (LC-MS/MS). The randomly collected samples (over 14 days) were extracted with acetonitrile and purified using a Florisil solid-phase extraction (SPE) cartridge. Linearity over a concentration range of 0.05-50.0 mg/L had an excellent coefficient of determination (R(2) ) = 0...
July 21, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28722757/discrimination-of-polygoni-multiflori-radix-and-cynanchi-auriculati-radix-using-ultra-high-performance-liquid-chromatography-fingerprints-and-chemical-pattern-recognition
#2
Lili Sun, Meng Wang, Yali Liu, Huijie Zhang, Yanan Liu, Xiaoliang Ren, Yanru Deng
In this work, a strategy was proposed to discriminate Polygoni Multiflori Radix (PMR) and its adulteration (Cynanchi Auriculati Radix, CAR). The ultra-high performance liquid chromatography (UHPLC) fingerprints were established to analyze samples containing PMR, CAR and mixtures simultaneously. Multivariate classification methods were applied to analyze the obtained UHPLC fingerprints, including principal component analysis (PCA), partial least square discriminant analysis (PLS-DA), soft independent modeling of class analogy (SIMCA), support vector machine discriminant analysis (SVMDA), and counter-propagation artificial neural network (CP-ANN)...
July 19, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28722197/simultaneous-determination-and-method-validation-of-difenoconazole-propiconazole-and-pyraclostrobin-in-pepper-and-soil-by-lc-ms-ms-in-field-trial-samples-from-three-provinces-china
#3
Kunming Zheng, Banghua Meng, Sizhuo Wu, Haizhen Zhang, Fei Wang, Ying Cui, Song Zeng, Kankan Zhang, Deyu Hu
A liquid chromatography-electrospray ionization tandem mass spectrometry method was developed for simple and accurate detection of the fungicides difenoconazole, propiconazole and pyraclostrobin in peppers and soil. Three fungicides residues were extracted from samples by acetonitrile, cleaned up by dispersive solid phase extraction before instrumental analysis. The accuracy and precision of the method were evaluated by conducting intra- and inter-day recovery experiment. The limits of quantification and detection of difenoconazole, propiconazole and pyraclostrobin in pepper and soil were 0...
July 19, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28722154/application-of-headspace-solid-phase-microextraction-followed-by-gas-chromatography-coupled-with-mass-spectrometry-spme-gc-ms-to-determine-esters-of-carboxylic-acids-and-other-volatile-compounds-in-d-maculatus-and-d-ater-lipids
#4
Magdalena Cerkowniak, Mieczysława I Boguś, Emilia Włóka, Piotr Stepnowski, Marek Gołębiowski
A constant problem in veterinary medicine, human healthcare, agriculture, forestry or horticulture is the large number of pests, and the lack of effective methods to combat them which cause no harm to the rest of the environment. It is recommended and desired to reduce the use of chemicals and increase the use of agents based on the knowledge acquired in the field of biology, chemistry, and agrochemicals. To learn the defense mechanisms of insects we should consider not only the site of their physiological ability to protect against external factors (cuticle), but also check the possibility of chemical protection, formed by all compounds on the surface and in the body of insects...
July 19, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28718960/chemical-uplc-esi-ms-ms-profiling-of-aconitum-alkaloids-and-their-metabolites-in-rat-plasma-and-urine-after-oral-administration-of-aconitum-carmichaelii-debx-root-extract
#5
Mingjie Zhang, Manman Wang, Jiajia Liang, Yongqing Wen, Zhili Xiong
In this paper, an ultra high performance liquid chromatography tandem mass spectrometric (UPLC-ESI-MS/MS) method in positive ion mode was established to systematically identify and to compare the major aconitum alkaloids and their metabolites in rat plasma and urine after oral administration of Fuzi extract. A total twenty-nine components including twenty-five C19 -diterpenoid alkaloids and four C20 -diterpenoid alkaloids were identified in Fuzi extract. Thirteen of the parent components and five metabolites were detected in rat plasma and sixteen parent compounds and six metabolites in urine...
July 18, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28707762/pharmacokinetics-and-tissue-distribution-study-of-clevidipine-and-its-primary-metabolite-h152-81-in-rats
#6
Yan Wang, Lanting Zhao, Tengfei Li, Wen Yang, Qian Li, Luning Sun, Li Ding
This present study was designed to investigate the pharmacokinetic profiles and tissue distribution characteristics of clevidipine and its primary metabolite H152/81 in rats following a single intravenous administration of clevidipine butyrate injectable emulsion (CBIE). For this study, a sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established and validated for the simultaneous quantitation of clevidipine and H152/81 in rat whole blood and various tissues. A Hedera ODS-2 column with two gradient elution programs was employed for the troubleshooting of matrix effect on the detection of analytes among different biological samples...
July 14, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28702943/screening-and-identification-of-metabolites-of-two-kinds-of-main-active-ingredients-and-hepatotoxic-pyrrolizidine-alkaloids-hpas-in-rat-after-lavage-farfarae-flos-extract-by-uhplc-q-tof-ms-mass-spectrometry
#7
Xiaoye Cheng, Man Liao, Xinpeng Diao, Yupeng Sun, Lantong Zhang
Farfarae Flos, the dried flower buds of Tussilago farfara L., is usually used to treat coughs, bronchitic and asthmatic conditions as an important traditional Chinese medicine. Tussilagone and methl butyric acid tussilagin ester are seen as representatives of two kinds of active substances. Besides, the pyrrolizidine alkaloids (HPAs), mainly senkirkine and senecionine, presented in the herb can be hepatoxic. In this study, a rapid and sensitive ultra high performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) method is successfully applied to identify the metabolites of tussilagone, methl butyric acid tussilagin ester , senkirkine and senecionine...
July 12, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28682490/an-lc-ms-ms-method-for-quantitation-of-cyanidin-3-o-glucoside-in-rat-plasma-application-to-a-comparative-pharmacokinetic-study-in-normal-and-streptozotocin-induced-diabetic-rats
#8
Chunxia Yang, Qiuhua Wang, Shenbao Yang, Qiong Yang, Ying Wei
A sensitive and reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed to determine cyanidin-3-O-glucoside (Cy-3G) in normal and streptozotocin-induced diabetic rat plasma. Chromatographic separation was carried out on a ZORBAX SB-C18 (50 mm × 4.6 mm, 5 μm) column and mass spectrometric analysis was performed using a Thermo Finnigan TSQ Quantum Ultra triple-quadrupole mass spectrometer coupled with an ESI source in the negative ion mode. Selected reaction monitoring (SRM) mode was applied for quantification using target fragment ions m/z 447...
July 6, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28677911/characterization-and-discrimination-of-raw-and-vinegar-baked-bupleuri-radix-based-on-uhplc-q-tof-ms-coupled-with-multivariate-statistical-analysis
#9
Tianli Lei, Shifeng Chen, Kai Wang, Dandan Zhang, Lin Dong, Chongning Lv, Jing Wang, Jincai Lu
Bupleuri Radix is a commonly used herb in clinic, and raw and vinegar-baked Bupleuri Radix are both documented in the Pharmacopoeia of People's Republic of China. According to the theories of traditional Chinese medicine, Bupleuri Radix possesses different therapeutic effects before and after processing. However, the chemical mechanism of this processing is still unknown. In this study, an ultra-high performance liquid chromatography with quadruple time-of-flight mass spectrometry coupled with multivariate statistical analysis including principal component analysis and orthogonal partial least square-discriminant analysis was developed to holistically compare the difference between raw and vinegar-baked Bupleuri Radix for the first time...
July 5, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28677899/validated-lc-ms-ms-method-for-quantitation-of-demethylbellidifolin-in-rat-plasma-and-its-application-to-pharmacokinetic-and-bioavailability-studies
#10
Jie Zhang, Huiyu Yan, Xiaoyu Qu, Wei Zhou
Demethylbellidifolin, a major xanthone compound of Swertia davidi Franch, showed many beneficial pharmacological effects including antioxidation, anti-inflammation, anti-fibrosis, and cardiovascular protection effects. In this research, a rapid and sensitive LC-MS/MS method for the quantitative analysis of demethylbellidifolin in rat plasma was developed. The demethylbellidifolin and internal standard of aurantio-obtusin were extracted from 50 μL of rat plasma samples with ethyl acetate, then the dried residue was reconstituted and injected in an HPLC system with ZORBAX SB-C18 analytical column (2...
July 5, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28677837/determination-of-tigecycline-in-human-plasma-by-lc-ms-ms-and-its-application-to-population-pharmacokinetics-study-in-chinese-patients-with-hospital-acquired-pneumonia
#11
Rong Shao, Xingang Li, Yangmin Hu, Jinliang Chen, Honggang Lou, Haibin Dai
A selective, sensitive, and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of Tigecycline (TGC) in human plasma, using Tigecycline-d9 as an internal standard (IS). Analytical samples were prepared using a protein precipitation method coupled with a concentration process. The analyte and IS were separated on a reversed-phase Waters Acquity UPLC® BEH-C18 column (2.1 mm × 50 mm ID, 1.7 μm) with a flow rate of 0.25 ml/min. The mobile phase consisted of water, containing 0...
July 5, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28677821/development-and-validation-of-a-high-performance-liquid-chromatography-method-for-the-quantification-of-talazoparib-in-rat-plasma-application-to-plasma-protein-binding-studies
#12
Mahendra Kumar Hidau, Srikanth Kolluru, Srinath Palakurthi
A sensitive and selective RP-HPLC method has been developed and validated for the quantification of a highly-potent poly ADP ribose polymerase (PARP) inhibitor talazoparib (TZP) in rat plasma. Chromatographic separation was performed with isocratic elution method. Absorbance for TZP was measured with UV detector (SPD-20A UV-VIS) at λmax of 227 nm. Protein precipitation method was used to extract the drug form plasma samples using methanol: acetonitrile (65:35) as a precipitating solvent. Method was shown sensitive and reproducible over 100-2000 ng/mL linearity range with LLQC of 100 ng/mL...
July 5, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28670837/analytical-methodologies-for-the-stereoselective-determination-of-fluoxetine-an-overview
#13
REVIEW
Gabriel Hancu, Melania Cârcu-Dobrin, Monica Budău, Aura Rusu
Fluoxetine is a widely used antidepressant belonging to the selective serotonin reuptake inhibitor class; used in the treatment of major depression, obsessive compulsive, premenstrual dysphoric, panic and post-traumatic stress disorders. Fluoxetine is an optical active pharmaceutical substance, which is used as a racemate in therapy, but stereospecific interactions associated with the serotonin-reuptake carrier, for both the parent drug and its active metabolite, norfluoxetine, has been described in the literature...
July 3, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28670733/determination-of-silodosin-and-its-active-glucuronide-metabolite-kmd-3213g-in-human-plasma-by-lc-ms-ms-for-a-bioequivalence-study
#14
Priyanka A Shah, Pranav S Shrivastav
A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is described for the simultaneous determination of silodosin (SLD) and its active metabolite silodosin β-d-glucuronide (KMD-3213G) in human plasma. Liquid-liquid extraction of plasma samples was carried out with ethyl acetate and methyl tert-butyl ether solvent mixture using deuterated analogs as internal standards. The extraction recoveries of SLD and KMD-3213G were in the ranges 90.8-93.4 and 87.6-89.9%, respectively...
July 3, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28664620/application-of-gas-and-liquid-chromatography-coupled-to-time-of-flight-mass-spectrometry-in-pesticides-multi-residue-analysis
#15
Abdalla Ahmed Elbashir, Hassan Y Aboul-Enein
Analysis of pesticide residues in water and food matrices are active research area closely related to food safety and environmental issues. In this aspect mass spectrometry (MS) coupled to gas chromatography (GC) and liquid chromatography (LC) has been increasingly used in the analysis of pesticides residues in water and food. The increasing interest in application of high resolution mass spectrometry (HRMS) with time-of-flight (TOF) and the hybrid QqTOF in the pesticides analysis is due to its capability of performing both targeted and non-targeted analysis...
June 29, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28664581/elevated-levels-of-liver-methylglyoxal-and-d-lactate-in-early-stage-hepatitis-in-rats
#16
Wen-Chuang Wang, Chu-Kuang Chou, Ming-Cheng Chuang, Yi-Chieh Li, Jen-Ai Lee
Methylglyoxal (MGO) is highly cytotoxic and its levels are elevated in diabetes, nephropathy, and atherosclerosis. However, it has never been studied in liver disease. For this reason, we aimed to assess the levels of MGO and its metabolite D-lactate in an early hepatitis model. Wistar rats were administered CCl4 (0.75 ml/Kg, i.p.) to induce hepatitis. In either CCl4 -treated or untreated rats, AST and ALT levels did not change over the course of the study, indicating that significant liver damage did not occur following CCl4 treatment...
June 29, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28664556/a-uplc-tof-ms-based-metabolomics-study-of-rattan-stems-of-schisandra-chinensis-effects-on-alzheimer-s-disease-rats-model
#17
Bing-You Yang, Jin-Yan Tan, Yan Liu, Bo Liu, Shuang Jin, Hong-Wei Guo, Hai-Xue Kuang
A UPLC-TOF/MS-based metabolomics method was established to explore the therapeutic mechanisms of rattan stems of Schisandra. Chinensis (SCS) on Alzheimer's disease (AD). Experimental AD model was induced by intra-hippocampal Aβ1-42 injection in rats. Cognitive function and oxidative stress condition in brain of AD rats were assessed using Morris water maze tests and antioxidant assays (MDA, SOD and GSH-Px), respectively. UPLC-TOF/MS combined with multivariate statistical analysis were conducted to study the changes of metabolic networks in serum of rats...
June 29, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28664536/metabolomics-revealed-the-toxicity-of-cationic-liposomes-in-hepg2-cells-using-uhplc-q-tof-ms-and-multivariate-data-analysis
#18
Jing Yu, Hai Zhang, Ying Li, Sen Sun, Jie Gao, Yanqiang Zhong, Duxin Sun, Guoqing Zhang
Cationic liposomes (CLs) are novel non-viral vectors widely used for delivering drugs or genes. However, applications of CLs are largely hampered by their cytotoxicity, partly because the potential mechanism underlying the cytotoxicity of CLs remains unclear. The aim of the present study was to explore the underlying mechanism of cytotoxicity induced by CLs on HepG2 cells. Differential metabolites were identified and quantified using ultra-liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS)...
June 29, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28636139/review-of-hplc-and-lc-ms-ms-assays-for-the-determination-of-various-non-steroidal-anti-androgens-nsaa-used-in-the-treatment-of-prostate-cancer
#19
REVIEW
P S Suresh, Nuggehally R Srinivas, Ramesh Mullangi
Prostate cancer is the most common cancer and one of the leading causes for cancer deaths in men. One of the commonly used approaches to treat metastatic prostate cancer was via first generation non-steroidal anti-androgens (NSAA) namely flutamide, nilutamide, bicalutamide and topilutamide. Most of the prostate cancer patients who are initially responsive develop a most aggressive form of disease called castration-resistant prostate cancer (CRPC). Second generation NSAA receptor antagonists (enzalutamide, apalutamide and darolutamide) are emerging as additional new options to treat CRPC...
June 21, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28623874/lansoprazole-sulfide-pharmacokinetics-of-this-promising-anti-tuberculous-agent
#20
Sipho Mdanda, Sooraj Baijnath, Adeola Shobo, Sanil D Singh, Glenn E M Maguire, Hendrik G Kruger, Per I Arvidsson, Tricia Naicker, Thavendran Govender
Lansoprazole (LPZ) is a commercially available proton-pump inhibitor whose primary metabolite, lansoprazole sulfide (LPZS) was recently reported to have in vitro and in vivo activity against Mycobacterium tuberculosis. It was also reported that a 300 mg kg(-1) oral administration of LPZS was necessary to reach therapeutic levels in the lung, with the equivalent human dose being unrealistic. A validated liquid chromatography-tandem mass spectrometric method (LC-MS/MS) for the simultaneous quantification LPZ and LPZS in rat plasma and lung homogenates was developed...
June 17, 2017: Biomedical Chromatography: BMC
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