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Biomedical Chromatography: BMC

Sonali G Thorat, Rupesh V Chikhale, Madhukar R Tajne
Capecitabine is a prodrug of 5-flurouracil, employed as a broad spectrum chemotherapeutic agents. It is also used as monotherapy or a combination chemotherapy agent for treatment of colorectal cancer. Capecitabine is administered in combination with oxaliplatin and hence it becomes essential to determine that co-administration does not affect its metabolism. To determine the plasma concentration of capecitabine a simple HPTLC method was developed and validated. Blood samples from 12 patients with colorectal cancer were collected and analysed by the HPTLC method with a reference internal standard...
September 22, 2017: Biomedical Chromatography: BMC
Yuki Shirayama, Kosuke Doki, Yukio Sekiguchi, Kazutaka Aonuma, Yukinao Kohda, Masato Homma
Propafenone, a class Ic antiarrhythmic agent, is metabolized to 5-hydroxypropafeone (5-OHP) and N-depropylpropafenone (NDPP). Simultaneous determination of serum propafenone and its metabolites was performed using high-performance liquid chromatography (HPLC) equipped with a conventional octadecylsilyl silica column and ultraviolet detector. The wavelength was set at 250 nm. Propafenone and its metabolites in the serum were extracted using diethyl ether. The mobile phase solution, comprising 1-pentanesulfonic acid sodium salt (0...
September 19, 2017: Biomedical Chromatography: BMC
Waziha Farha, A M Abd El-Aty, Md Musfiqur Rahman, Md Humayun Kabir, Hyung Suk Chung, Han Sol Lee, Jong-Sup Jeon, Jing Wang, Byung-Joon Chang, Ho-Chul Shin, Jae-Han Shim
This study aimed at quantifying the residual amount of azoxystrobin in Swiss chard samples grown under greenhouse conditions at 2 different locations (Gwangju and Naju, Republic of Korea). Samples were extracted with acetonitrile, separated by salting out, and subjected to purification by using solid-phase extraction (SPE). The analyte was identified using liquid chromatography (LC)-ultraviolet detector (UVD). The linearity of the calibration range was excellent with coefficient of determination (R(2) ) =1...
September 15, 2017: Biomedical Chromatography: BMC
Dan Wei, Yan Zhu, Ming Guo
A sequential on-line extraction, clean-up and separation system for the determination of betaine, L-carnitine and choline in human urine using column-switching ion chromatography with non-suppressed conductivity detection was developed in this work. Self-packed pretreatment column (50 mm×4.6 mm, i.d.) was used for the extraction and clean-up of betaine, L-carnitine and choline. The separation was achieved using self-packed cationic exchange column (150 mm×4.6 mm, i.d.), followed by non-suppressed conductivity detection...
September 15, 2017: Biomedical Chromatography: BMC
Haiyan Zhu, Mingchang Chen, Xunlong Shi, Chenchen Shi, Chenggang Huang
Tanreqing Injection (TRQ) has been used primarily in treating infections of the upper respiratory tract and serious influenza in China, as a classical compound herbal recipe. TRQ had been demonstrated its effects of clearing heat, eliminating phlegm, detoxification, reducing inflammation and alleviating cough. The survival rate, histopathology of lungs and viral titers in mice were evaluated in this study to verify the curative effect of TRQ. However, there is not enough information about the components. In the present study, a high-performance and practical LC/QTOF/MS method was developed for characterization and identification of the natural ingredients in TRQ...
September 15, 2017: Biomedical Chromatography: BMC
Raphael S Affonso, Josélia A Lima, Bruno Lessa, João V O Caetano, Marcos T Obara, Andrea Nóbrega, Eugenie Nepominova, Kamil Musilek, Kamil Kuca, Gláucia Barbosa Candido Alves Slana, Tanos C C França
The rise of mosquito's transmitted diseases, like dengue, zika and chikungunya in Brazil in the last years has increased the concerns on protection against mosquito's bites. However, the prohibitive prices of the commercially available repellents for the majority of the Brazilian population, has provoked a search for cheaper solutions, like the use of the homemade ethanolic extract of Indian clove (Syzygium aromaticum L.) as repellent, which has been reported as quite efficient by the local press. In order to verify this, we performed here, the quantification of the main components of this extract through high-performance thin-layer chromatography (HPTLC)-densitometry and evaluated its efficiency as repellent, as well as the acetylcholinesterase (AChE) inhibition capacity...
September 15, 2017: Biomedical Chromatography: BMC
Lang Lang, Zhaorui Meng, Lan Sun, Wei Xiao, Longshan Zhao, Zhili Xiong
Guizhi Fuling capsule (GFC), developed from the traditional Chinese prescription of Guizhi Fuling Wan, has been commonly used for the treatment of primary dysmenorrheal (PD). However, the intervention effective mechanism in vivo has not been well elucidated. In this study, an integrated plasma metabonomic strategy based on RP-UPLC-MS coupled with HILIC-UPLC-MS technique has been developed to investigate the global therapeutic effects and intervention mechanisms of Guizhi Fuling capsule (GFC) on dysmenorrhea rats induced by oxytocin...
September 14, 2017: Biomedical Chromatography: BMC
Guogai Zhang, Lifang Zhao, Jiancheng Zhu, Yifan Feng, Xia Wu
Alpinia officinarum was used for anti-inflammatory activity historically in China. Diarylheptanoids isolated from Alpinia officinarum play important biological roles in prevention and treatment of inflammatory disorders. Seven diarylheptanoids (1-7) were isolated from Alpinia officinarum. The cell viabilities and anti-inflammatory activities of diarylheptanoids were evaluated by MTT assay and tumor necrosis factor-a (TNF-a) production in KLA-stimulated RAW 264.7 cells in vitro. Meanwhile, the relationships between their anti-inflammatories and structure-activities were discussed...
September 14, 2017: Biomedical Chromatography: BMC
Pravin G Vanol, Manish Yadav, Mallika Sanyal, Priyanka A Shah, Pranav S Shrivastav
An improved, precise and reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the quantification of trimetazidine, using trimetazidine-d8 as the internal standard (IS). Interference due to plasma phospholipids during sample preparation was overcome by using hybrid solid phase extraction-phospholipid ultra cartridge. The mean extraction recovery of trimetazidine (98.66 %) and trimetazidine-d8 (97.63 %) from spiked plasma was consistent and reproducible...
September 14, 2017: Biomedical Chromatography: BMC
Haizhen Zhang, A'wei Zhang, Min Huang, Weiwei Yu, Zhurui Li, Sizhuo Wu, Kunming Zhang, Kankan Zhang, Deyu Hu
A gas chromatography-tandem mass spectrometry method was developed and validated to simultaneously determine boscalid and fludioxonil in grape and soil samples. These samples were extracted with 10 mL of acetonitrile and purified using a mixed primary secondary amine/octadecylsilane sorbent. The method showed good linearity (R(2) >0.99) in the calibration range 0.005-2 μg/mL for both pesticides. The limits of detection and quantification for the two analytes in grape and soil were 0.006 mg/kg and 0.02 mg/kg, respectively...
September 14, 2017: Biomedical Chromatography: BMC
Sonika Batra, Ravi Bhushan
Atenolol, a β-adrenergic receptor antagonist, is a chiral compound used for the treatment of cardiovascular diseases and to treat hypertension, coronary heart disease, arrhythmias, sinus tachycardia and myocardial infarction, where it acts preferentially upon the β-adrenergic receptors in the heart. It is marketed as a racemate, but only (S)-enantiomer of (RS)-atenolol is responsible for the β-adrenoceptor blocking activity. Different chromatographic methods have been applied for separation and determination of enantiomers...
September 14, 2017: Biomedical Chromatography: BMC
Weili Huang, Xiaohui Guan, Yongchen Lv
A specific and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for the analysis of glaucocalyxin A and glaucocalyxin B in rat plasma using praeruptorin A as an internal standard. Separation was performed on a Hypurity C18 column (2.1 mm × 50 mm, 5 μm) with isocratic elution using 0.2% formic acid in water-acetonitrile (20:80, v/v). Mass spectrometric detection was conducted using selected reaction monitoring via an electrospray ionization source. Both analytes exhibited good linearity within their concentration ranges (r(2) > 0...
September 5, 2017: Biomedical Chromatography: BMC
N M El Zahar, N Magdy, A M El-Kosasy, Michael G Bartlett
Phosphorothioate (PS) oligonucleotides are a rapidly rising class of drugs with significant therapeutic applications. However, due to their complex structure and multi-step synthesis and purification processes, generation of low level impurities and degradation products are common. Therefore, they require significant investment in quality control and impurity identification. This requires the development of advanced methods for analysis, characterization and quantitation. In addition, the presence of the PS linkage leads to the formation of chiral centers which can affect their biological properties and therapeutic efficiency...
September 4, 2017: Biomedical Chromatography: BMC
Jing Wei, Jun Gao, Xinghong Ding
A urine metabolomics method based on gas chromatography mass spectrometry (GC-MS) was developed in order to investigate the metabolites characters of systemic lupus erythematosus (SLE) and therapeutic effects of jieduquyuziyin prescription (JP). The urinary metabolic profiles in urine specimens of the SLE model mice (MRL/lpr) group, prednisone acetate (PA)-treated SLE mice group, JP-treated SLE mice group, and control group (C57BL/6 J) after the administration were analyzed by GC-MS. These metabolic profiles were then processed by multivariate analysis, in particular Mass Profiler Professional (MPP), SIMCA-P and partial least-squares discriminant analysis (PLS-DA)...
September 4, 2017: Biomedical Chromatography: BMC
Sneha G Nair, Daxesh P Patel, Mallika Sanyal, Puran Singhal, Pranav S Shrivastav
A highly sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry method is described for the simultaneous determination of nomegestrol acetate (NOMAC), a highly selective progestogen, and estradiol (E2), a natural estrogen in human plasma. NOMAC was obtained from plasma by solid phase extraction, while E2 was first separated by liquid-liquid extraction with methyl tert-butyl ether followed by derivatization with dansyl chloride. Deuterated internal standards, NOMAC-d5 and E2-d4 were used for better control of extraction conditions and ionization efficiency...
September 4, 2017: Biomedical Chromatography: BMC
Sufang Shui, Xiaorong Cai, Rongqing Huang, Bingkun Xiao, Jianyun Yang
Yi Guan Jian (YGJ), one of the most commonly used traditional Chinese medicines (TCM), has been reported to possess significant antifatigue effects in the eastern. However, the mechanisms underlying its antifatigue effects remain largely unresolved. In this study, a metabonomics approach, involving gas chromatography coupled to mass spectrometry (GC/MS) and a multivariate statistical technique, was developed to estimate the extent to which YGJ alleviated the exhausting swimming induced fatigue of mice. High dose treatment with YGJ significantly extended the swimming time of fatigued mice...
September 3, 2017: Biomedical Chromatography: BMC
Ana Novak, Mercè Gutiérrez-Zamora, Lluís Domenech, Josep M Suñé-Negre, Montserrat Miñarro, Encarna García-Montoya, Josep M Llop, Josep R Ticó, Pilar Pérez-Lozano
A simple analytical method for simultaneous determination of phytosterols, cholesterol, and squalene in lipid emulsions was developed due to increased interest in their clinical effects. Method development was based on commonly used stationary (C18 , C8 and phenyl) and mobile phases (mixtures of acetonitrile, methanol, and water) under isocratic conditions. Differences in stationary phases resulted in peak overlapping or coelution of different peaks. The best separation of all analyzed compounds was achieved on Zorbax Eclipse XDB C8 (150 x 4...
August 30, 2017: Biomedical Chromatography: BMC
Ying Wang, Yifan Zhang, Juan Xiao, Ranchi Xu, Qiangli Wang, Xinhong Wang
A rapid and high sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for simultaneous determination of nine active constituents, baicalin, baicalein, wogonoside, wogonin, scutellarin, berberine, coptisine, ginsenoside Rb1 and ginsenoside Re in rat plasma after oral administration of Banxia xiexin decoction(BXD). Biological samples were processed wtih acetone-ethyl acetate (4:1, v/v). The mobile phase consisted of methanol and water (containing 0.1% formic acid) with gradient elution at a flow rate of 0...
August 28, 2017: Biomedical Chromatography: BMC
Zhuo Wang, Linjun You, Yan Cheng, Kaiyong Hu, Zhanbo Wang, Yanan Cheng, Jin Yang, Yong Yang, Guangji Wang
Schisandrin B has received much attention owing to its various biological activities. The present study was aimed at the formulation development of schisandrin B and investigation of the pharmacokinetic profiles, distribution and excretion of schisandrin B in Sprague-Dawley rats. In this study, micronized schisandrin B particles with particle size of 10-20 μm were chosen as the research object. Chromatographic separation was carried out on a BDS Hypersil C18 column (50 × 2.1 mm, i.d. 3.5 μm). Schisandrin B and deoxyschizandrin (internal standard) were detected without interference in the multiple reaction monitoring mode with positive electrospray ionization...
August 22, 2017: Biomedical Chromatography: BMC
Wen Xu, Quankun Zhuang, Xia Chen, Ji Jiang, Pei Hu, Hongyun Wang
A robust ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of morphine-6-D-glucuronide (M6G), morphine-3-D-glucuronide (M3G) and morphine (MOR) in human plasma and urine has been developed and validated. The analytes of interest were extracted from plasma by protein precipitation. Urine sample was prepared by dilution. Both plasma and urine samples were chromatographed on an Acquity UPLC HSS T3 column using gradient elution. Detection was performed on a Xevo TQ-S tandem mass spectrometer in multiple reactions monitoring (MRM) mode using positive electrospray ionization (ESI)...
August 19, 2017: Biomedical Chromatography: BMC
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