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Biomedical Chromatography: BMC

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https://www.readbyqxmd.com/read/28500645/development-and-validation-of-an-lc-esi-ms-ms-approach-to-determinate-a-highly-hydrophobic-drug-norcantharidin-palmitate-and-apply-to-a-preliminary-pharmacokinetic-study-in-rats
#1
Xiaolin Liu, Xiaoguang Tao, Qi Zheng, Hang Xu, Yu Zhang, Tian Lei, Tian Yin, Haibing He, Xing Tang
In order to investigate the pharmacokinetics of norcantharidin palmitate (NCTD-PAL) in rats, we developed and validated an LC-ESI-MS/MS method. The NCTD-PAL and internal standard (Triamcinoloneacetonide palmitate, TAP) were separated on a Phenomenex Kinetex®XB C18 column, and the mobile phase was composed of tetrahydrofuran (THF)-acetonitrile (20/80, v/v) and an aqueous phase containing 0.2% ammonium hydroxide at a flow rate of 0.3 mL/min. The ESI interface operated in positive mode was used to acquire the mass spectrometric data, and the transition ions were m/z 635...
May 12, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28493452/suitability-of-selected-chromatographic-columns-for-analysis-of-fatty-acids-in-dialyzed-patients
#2
Magdalena Pazda, Piotr Stepnowski, Tomasz Sledzinski, Michal Chmielewski, Adriana Mika
Gas chromatography-mass spectrometry (GC-MS) is a preferred method for fatty acid (FA) analysis in biofluids from patients with metabolic diseases. Complex characteristics of FAs make their analysis particularly challenging. Selection of an appropriate chromatographic column is particularly important component of the process as it provides optimal separation and detection of possibly all FAs present in the sample. However, no accurate protocol for comparative evaluation of capillary columns for the analysis of whole serum FA profile in patients with chronic kidney disease (CKD) has been developed thus far...
May 10, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28493441/uhplc-esi-ms-ms-determination-and-pharmacokinetics-of-pinoresinol-glucoside-and-chlorogenic-acid-in-rat-plasma-after-oral-administration-of-eucommia-ulmoides-oliv-extract
#3
Xiaojian Gong, Qingxiang Luan, Xin Zhou, Yang Zhao, Chao Zhao
This study aimed to develop a specific UHPLC-ESI-MS/MS method for simultaneous determination and pharmacokinetics of pinoresinol glucoside and chlorogenic acid in rat plasma after oral administration of E. ulmoides. The chromatographic separation was achieved on a Hypersil GOLD column with gradient elution by using a mixture of 0.1% formic acid aqueous solution and acetonitrile as the mobile phase at a flow rate of 200 μL/min. A tandem mass spectrometric detection was conducted using multiple-reaction monitoring (MRM) via an electrospray ionization (ESI) source in negative ionization mode...
May 10, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28493423/development-and-validation-of-a-sensitive-uhplc-ms-ms-method-for-quantitative-analysis-of-farrerol-in-rat-plasma-application-to-pharmacokinetic-and-bioavailability-studies
#4
Li Piao, Mingcui Zang, Yue Gu, Baohua Liu
Farrerol is a 2,3-dihydro-flavonoid isolated from rhododendron. In this study, a sensitive and selective ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for the determination of farrerol in rat plasma. Liquid-liquid extraction by ethyl ether was used for sample preparation. Chromatographic separation was achieved on an Agilent UHPLC XDB-C18 column (2.1 mm × 100 mm, 1.8 µm) with water and methanol (30:70, v/v) as the mobile phase. An electrospray source was applied and operated in negative ion mode; selection reaction monitoring was used for quantification using target fragment ions m/z 299 → 179 for farrerol and m/z 267 → 252 for internal standard...
May 10, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28493402/development-and-validation-of-hplc-ms-ms-method-for-the-determination-of-lixivaptan-in-mouse-plasma-and-its-application-in-a-pharmacokinetic-study
#5
Adnan A Khady, Haithem Alrabiah, Mohamed W Attwa, Sabray Attia, Gamal A E Mostafa
The aim of the present study was to develop a simple, sensitive, and accurate liquid chromatography-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for the determination of lixivaptan (LIX) in mouse plasma by using vildagliptin as the internal standard (IS). A precipitation procedure was used for the extraction of LIX and vildagliptin from mouse plasma. Chromatographic separation of LIX was achieved using a C18 analytical column (50 mm × 2.1 mm, 1.8 µm) at 25 °C. The mobile phase comprised of acetonitrile and ammonium formate (10 mM, pH 3...
May 10, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28493358/structural-identification-of-degradants-of-moexipril-by-lc-ms-ms
#6
C Purushotham Reddy, K Ramakrishna, K M V Narayana Rao
A gradient LC-MS method was developed for the identification and characterization of degradants of moexipril using liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). Moexipril was subjected to hydrolysis (acid, base and neutral), oxidation, photolytic and thermal degradation conditions as mentioned in ICH guidelines Q1A (R2). The drug degraded under hydrolysis, oxidation and photolytic conditions, but it was stable under thermal conditions. In total, five degradants were formed and separated on an Agilent XDB C-18 column (4...
May 10, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28485018/fast-and-parallel-determination-of-pcb-77-and-pcb-180-in-plasma-using-ultra-performance-liquid-chromatography-uplc-with-diode-array-detection-dad-a-pharmacokinetic-study-in-swiss-albino-mouse
#7
N Ramanujam, M Sivaselvakumar, S Ramalingam
A simple, sensitive and reproducible ultra-performance liquid chromatography (UPLC) method has been developed and validated for simultaneous estimation of PCB 77 and PCB 180 in mouse plasma. The sample preparation was performed by simple liquid-liquid extraction technique. The analytes were chromatographed on a Waters Acquity H class UPLC system using isocratic mobile phase conditions at a flow rate of 0.3 mL/min and Acquity UPLC BEH shield RP18 column maintained at 35 °C temperature. Quantification was performed on a photodiode array detector set at 215 nm and PCB 101 was used as internal standard (IS)...
May 8, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28481409/development-and-validation-of-a-liquid-chromatography-tandem-mass-spectrometry-method-for-the-measurement-of-urinary-catecholamines-in-diagnosis-of-pheochromocytoma
#8
Ying Shen, Liming Cheng, Qing Guan, Huijun Li, Jie Lu, Xu Wang
The measurement of catecholamines in human body fluids is requested frequently for the differential diagnosis and monitoring of pheochromocytoma. The methods in most clinical laboratories focus on high performance liquid chromatography coupled with electrochemical detection which suffers from high background noise, low sensitivity, and poor separation. We reported and developed a robust high-throughput liquid chromatography tandem mass spectrometry method in routine clinical laboratories for the measurement of urinary catecholamines for diagnosis of pheochromocytoma...
May 8, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28477383/screening-free-radical-scavengers-in-xiexin-tang-by-hplc-abts-dad-q-tof-ms
#9
Yu-Qing Wang, Shu-Jiao Li, Guo Zhuang, Rong-Hui Geng, Xu Jiang
Xiexin Tang (XXT) is a traditional Chinese medicine (TCM) that has been used in herbal clinics for more than 1800 years. Many studies have shown that XXT has therapeutic effects on patients with arteriosclerosis (AS) owing to its antioxidant activity. However, there is little information about the relationship between the chemical composition of XXT and its antioxidant activity. In this study, the HPLC-ABTS-DAD-Q-TOF/MS method, which can simultaneously identify individual components and rapidly screen for antioxidant compounds, was used to screen and identify antioxidant components in XXT...
May 6, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28475242/a-simple-lc-ms-ms-method-facilitated-by-salting-out-assisted-liquid-liquid-extraction-salle-to-simultaneously-determine-trans-resveratrol-res-its-glucuronide-and-sulfate-conjugates-in-rat-plasma-and-its-application-to-pharmacokinetic-assay
#10
Zhixia Qiu, Jiaojiao Yu, Yu Dai, Yue Yang, Xiaoyu Lu, Jiaqiu Xu, Zhiying Qin, Fang Huang, Ning Li
A simple LC-MS/MS method facilitated by salting-out assisted liquid-liquid extraction (SALLE) was applied to simultaneously investigate pharmacokinetics of trans-resveratrol (Res), its major glucuronide and sulfate conjugates in rat plasma. Acetonitrile-methanol (80:20, v/v) and ammonium acetate (10 mol∙L(-1) ) were used as extractant and salting-out reagent to locate the target analytes in the supernatant after the aqueous and organic phase stratification, then the analytes were determined via gradient elution by LC-MS/MS in negative mode in a single run...
May 5, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28475217/identification-of-differential-metabolic-characteristics-between-tumor-and-normal-tissue-from-colorectal-cancer-patients-by-gas-chromatography-mass-spectrometry
#11
Wu Ning, Haijing Li, Fanqiang Meng, Jianhua Cheng, Xin Song, Guochao Zhang, Wenyue Wang, Shengming Wu, Junjian Fang, Kunpeng Ma, Jie Yang, Dongpo Pei, Fangting Dong
Colorectal cancer (CRC) is one of the most common human malignancies and encompasses cancers of the colon and rectum. Although the gold-standard colonoscopy screening method is effective in detecting CRC, this method is invasive and can result in severe complications for patients. The purpose of this study was to determine differences in metabolites between CRC and matched adjacent non-tumor tissues from CRC patients, to identify potential biomarkers that may be informative and developed screening methods. Metabolomic analysis was performed on clinically localized CRC tissue and matched adjacent non-tumor tissue from twenty CRC patients...
May 5, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28474782/a-comparative-pharmacokinetic-study-of-three-flavonoids-and-three-anthraquinones-in-normal-and-gastrointestinal-motility-disorders-rat-plasma-after-the-oral-administration-of-wei-chang-shu-tablet-using-high-performance-liquid-chromatography-tandem-mass-spectrometry
#12
Yan Ren, Weiwei Zhao, Juanjuan Zhao, Xiangming Chen, Chen Yu, Mengan Liu
A simple, fast and reliable high-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous quantification and pharmacokinetic study of three flavonoids (liquiritigenin, isoliquiritigenin and formononetin) and three anthraquinones (emodin, rhein and aloe-emodin), which are the bioactive ingredients of Wei-Chang-Shu tablet found in rat plasma. After extraction by liquid-liquid extraction with ethyl acetate, chromatographic separation was achieved on an Agilent ZORBAX SB-C18 column (4...
May 5, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28474759/a-simple-and-sensitive-high-performance-liquid-chromatography-electrochemical-detection-assay-for-the-quantitative-determination-of-monoamines-and-respective-metabolites-in-six-discrete-brain-regions-of-mice
#13
Serena A Allen, Stephanie Rednour, Samantha Shepard, Brooks Barnes Pond
A rapid, sensitive, and reproducible assay is described for the quantitative determination of the monoamine neurotransmitters dopamine, norepinephrine, and serotonin, their metabolites, and the internal standard 3,4- dihydroxybenzlyamine hydro-bromide (DHBA) in mouse brain homogenate using high performance liquid chromatography with electrochemical detection. The method was validated in the following brain areas: frontal cortex, striatum, nucleus accumbens, hippocampus, substantia nigra pars compacta, and ventral tegmental area...
May 5, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28449378/analysis-of-toldimfos-in-porcine-muscle-and-bovine-milk-using-liquid-chromatography-triple-quadrupole-mass-spectrometry
#14
Dan Zhang, Jin-A Park, A M Abd El-Aty, Seong-Kwan Kim, Sang-Hyun Cho, Weijia Zheng, Jeong-Min Choi, Jae-Han Shim, Byung-Joon Chang, Jin-Suk Kim, Ho-Chul Shin
An analytical method was developed for the detection of toldimfos sodium residues in porcine muscle and bovine milk using liquid chromatography-triple quadrupole tandem mass spectrometry (LC-MS/MS) analysis. The drug was extracted from muscle and milk using 10 mM ammonium formate in acetonitrile and then purified using n-hexane. The drug was well separated on a Luna C18 column using a mixture of 10 mM ammonium formate in ultrapure water (A) and acetonitrile (B) as the mobile phase. Good linearity was achieved over the tested concentration range (0...
April 27, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28440893/an-hplc-method-for-simultaneous-quantitative-determination-of-seven-secoiridoid-glucosides-separated-from-the-roots-of-ilex-pubescens
#15
Yang Zhang, Huan Xiong, Jiayi Bi, Guiyun Zhao, Yi Yang, Xue Chen, Yuncong Li, Chongyu Zhang, Guogang Zhang
A simple and specific high-performance liquid chromatographic method has been developed and validated to simultaneously determine seven secoiridoid glucosides for the first time. And three of them were separated from the ethanolic extract of the roots of Ilex pubescens for the first time, namely nuezhenide A, ligusides B and oleonuezhenide. In quantitative analysis, all of the calibration curves showed good linear regression (r > 0.999) within the tested ranges, and the mean recoveries of three different concentrations ranged from 97...
April 25, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28436051/a-novel-mass-spectrometry-based-method-for-simultaneous-determination-of-asymmetric-and-symmetric-dimethylarginine-l-arginine-and-l-citrulline-optimized-for-lc-ms-tof-and-lc-ms-ms
#16
Jerzy Wiśniewski, Mariusz G Fleszar, Joanna Piechowicz, Małgorzata Krzystek-Korpacka, Angelika Chachaj, Andrzej Szuba, Katarzyna Lorenc-Kukula, Leszek Masłowski, Wojciech Witkiewicz, Andrzej Gamian
Nitric oxide (NO) is a regulatory molecule involved in many biological processes. NO is produced by nitric oxide synthase by conversion of L-arginine to L-citrulline. L-arginine methylated derivatives, asymmetric and symmetric dimethylarginines (ADMA and SDMA), regulate L-arginine availability and the activity of nitric oxide synthase. As such, they have been frequently investigated as potential biomarkers in pathologies associated with dysfunctions in NO synthesis. Here, we present a new multistep analytical methodology based on liquid chromatography combined with mass spectrometry for the accurate identification of L-arginine, L-citrulline, ADMA, and SDMA...
April 24, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28432798/therapeutic-drug-monitoring-of-mitotane-analytical-assay-and-patient-follow-up
#17
Yoann Cazaubon, Helene Guillemin, Damien Vautier, Olivier Oget, Hervé Millart, Claire Gozalo, Zoubir Djerada, Catherine Feliu
BACKGROUND: Adrenocortical carcinoma (ACC) is an aggressive malignancy of the adrenal gland. Mitotane (o,p'-DDD) is the most effective chemotherapy for ACC. According to literature, mitotane plasma trough concentrations within 14-20 mg.L(1) are correlated with a higher response rate with acceptable toxicity. Therapeutic drug monitoring (TDM) of mitotane is therefore recommended. The aim of this study was to propose a robust, and simple method for mitotane quantification in plasma. The validation procedures were based on international guidelines...
April 22, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28431455/uhplc-q-tof-ms-ms-based-screening-and-characterization-of-metabolites-of-cnidilin-in-human-liver-microsomes
#18
Lin Yuan, Yuqian Zhang, Man Liao, Yanyan Liu, Changchen Wan, Lantong Zhang
Cnidilin is an active natural furocoumarin ingredient originating from well-known traditional Chinese medicine Radix Angelicae dahuricae. In the present study, an efficient approach was developed for the screening and identification of cnidilin metabolites using ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. In this approach, an on-line data acquisition method multiple mass defect filter (MMDF) combined with dynamic background subtraction was developed to trace all probable metabolites...
April 21, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28431453/carboxymethyl-%C3%AE-cyclodextrin-as-chiral-selector-in-capillary-electrophoresis-enantioseparation-of-16-basic-chiral-drugs-and-its-chiral-recognition-mechanism-associated-with-drugs-structural-features
#19
Linlin Fang, Yueying Du, Xiaoyu Hu, Linda Luo, Xin Guo, Xingjie Guo, Jia Yu
Herein we present the enantioseparation of 10 cardiovascular agents and 6 bronchiectasis drugs including propranolol, carteolol, metoprolol, atenolol, pindolol, esmolol, bisoprolol, bevantolol, arotinolol, sotalol, clenbuterol, procaterol, bambuterol, tranterol, salbutamol and terbutaline sulfate using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector. To our knowledge, there is no literature about using CM-β-CD for separating carteolol, esmolol, bisoprolol, bevantolol, arotinolol, procaterol, bambuterol and tranterol...
April 21, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28427113/simultaneous-quantification-and-semi-quantification-of-amentoflavone-and-its-metabolites-in-human-intestinal-bacteria-by-liquid-chromatography-orbitrap-high-resolution-mass-spectrometry
#20
Yiyun Qian, Shu Jiang, Zhenhua Zhu, Qi Wang, Shulan Su, Jinhua Tao, Jin-Ao Duan
A quick, easy, effective method followed by ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap MS) was developed for the simultaneous identification and quantification of the metabolites produced by Amentoflavone (AMF) in human intestinal bacteria from human feces. The method validated for quantification of AMF concerning precision, accuracy, recovery, matrix effect, stability and limits showed acceptable results. Compared the blank human intestinal bacteria chromatography, three metabolites were presumed based on high-accuracy protonated precursors and multi-stage mass spectrometry (MS(n) ) using the proposed strategy...
April 20, 2017: Biomedical Chromatography: BMC
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