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Biomedical Chromatography: BMC

Thidarat Intararuchikul, Narudol Teerapattarakan, Ratchanee Rodsiri, Mayuree Tantisira, Gert Wohlgemuth, Oliver Fiehn, Rossarin Tansawat
Centella asiatica has been used as culinary vegetable or medicinal herb. In this study, hepatoprotective effect of the standardized extract of Centella asiatica (ECa233) in rotenone-treated rats was examined using a GC-MS based metabolomic approach. ECa233 contains >80% triterpenoids with a ratio of madecassoside to asiaticoside of 1.5(±0.5):1. Rats were randomly divided into three groups (with six rats/group): sham negative control, rotenone positive control, and the ECa233 test group. Rats in the ECa233 group received 10 mg/kg ECa233 orally for 20 days, followed by 2...
September 22, 2018: Biomedical Chromatography: BMC
Haijuan Zhi, Zhenwei Li, Yanping Deng, Peng Qi, Jinjun Hou, Wen Deng, Jinle Cheng, Wanying Wu, Dean Guo
Salvia miltiorrhiza, one of the most famous herbal medicines, is commonly used for the treatment of coronary heart diseases in China. Besides traditional decoction pieces, another relatively new product of S. miltiorrhiza, Ultrafine Granular Powder (D90 < 45 μm), is also increasingly used nowadays. In this paper, a UHPLC-LTQ-Orbitrap MS technique was developed for metabolite profile study after oral administration of Ultrafine Granular Powder (UGP) and Traditional Decoction Slices (TDS) of S. miltiorrhiza...
September 21, 2018: Biomedical Chromatography: BMC
Priya Sharma, Devendra Kumar, Srikanth Mutnuri
To enable the reliable quantification of Ciprofloxacin in human urine, a sensitive and selective assay based on liquid chromatography-tandem mass spectrometry was developed. The chromatographic separation of the Ciprofloxacin was carried on a Zorbex Eclipse C18 column using Methanol and Ammonium acetate as a mobile phase by the gradient elution method. The developed assay covered a wide range of concentration (1.56-100 ng/ml) with a lower limit of detection of 0.76 ng/ml. Quantification was performed using the multiple reaction monitoring transitions 331...
September 21, 2018: Biomedical Chromatography: BMC
Bruno Alonso, Melissa Schwanz, Leticia Danielli, Miriam Apel, Amelia Henriques
Pomegranate is of current interest due to the existing potential for industrial uses of fruit peels. This includes its availability as a raw vegetable material, a byproduct that constitutes residue in the use of the species and is recognized as a functional product, and beneficial health properties, as will be demonstrated in the studies cited. Therefore, it is necessary to ensure its effectiveness and safety. Toward this end, the aim of this study was to develop and validate an analytical method for the separation and quantification of total punicalagin present in the bark of the fruit of Punica granatum by HPLC...
September 21, 2018: Biomedical Chromatography: BMC
Yashpal S Chhonker, Richard L Sleightholm, Daryl J Murry
Moxidectin (MOX) has recently been approved by the United States Food and Drug Administration for the treatment of river blindness in select populations. It is also being evaluated as an alternative for the use of Ivermectin for which wide spread resistance is becoming a global health issue. Moreover, MOX is becoming increasingly used as a prophylactic antiparasitic in the cattle industry. In this study, we developed and validated a LC-MS/MS method of MOX in human, monkey and mouse plasma. The separation was achieved on an ACE C18 (50 × 50 mm, 3μ) column with isocratic elution using 0...
September 20, 2018: Biomedical Chromatography: BMC
Weilun Hu, Qian Shao, Xingjun Xi, Qiao Chu, Tao Lan, Fenfang Che, Yuanyuan Liu, Yanzhen Lu, Yun Wei
In this work, a general and novel separation technique gas-assisted three-liquid-phase extraction was established and applied in separating and concentrating isoflavonoids from the actual sample of puerariae extract by one step. For the gas-assisted three-liquid-phase extraction method, optimal conditions were selected: polyethylene glycol 2000 and ethyl acetate as the flotation solvent, pH 5, (NH4 )2 SO4 concentration 350 g/L in aqueous phase, N2 flow rate 30 mL/min, flotation time 50 min, and twice flotation...
September 20, 2018: Biomedical Chromatography: BMC
Vanessa F M Carvalho, Daniela V Giaconi, Leticia V Costa-Lotufo, Edilberto Rocha Silveira, Luciana B Lopes
This study reports the development of a simple and reproducible method, with high rates of recovery, to extract the cytotoxic agent piplartine from skin layers, and a sensitive and rapid UV-HPLC method for its quantification. Considering the potential of piplartine for topical treatment of skin cancer, this method may find application for formulation development and pharmacokinetics studies to assess cutaneous bioavailability. Porcine skin was employed as a model for human tissue. Piplartine was extracted from the stratum corneum (SC) and remaining viable skin layers (VS) using methanol, vortex homogenization and bath sonication, and subsequently assayed by HPLC using a C18 column, and 1:1 (v/v) acetonitrile:water (adjusted to pH 4...
September 20, 2018: Biomedical Chromatography: BMC
Kyeong-Ryoon Lee, Song-Hee Chae, Min Ju Kim, Yoon-Jee Chae, Myung Yeol Lee, Chang Woo Lee, Jong Soon Kang, Won-Kee Yoon, Young-Suk Won, Kihoon Lee, Og-Sung Moon, Young-Kook Kim, Hyoung-Chin Kim
In this study, we developed a method for the determination of Penicillium griseofulvum-oriented pyripyropene A (PPPA), a selective inhibitor of acyl-coenzyme A:cholesterol acyltransferase 2, in mouse and human plasma and validated it using liquid chromatography-tandem mass spectrometry. Pyripyropene A (PPPA) and an internal standard, carbamazepine, were separated using a Xterra MS C18 column with a mixture of acetonitrile and 0.1% formic acid as the mobile phase. The ion transitions monitored in positive-ion mode [M+H]+ of multiple-reaction monitoring (MRM) were m/z 148...
September 20, 2018: Biomedical Chromatography: BMC
Xueqin Yang, Tao Zan, Huiyu Yan, Baohua Liu
A sensitive, selective and rapid ultra-performance liquid chromatography/tandem mass spectrometry method was developed and validated for the quantification of flavokawain B in rat plasma using myrislignan as an internal standard. Sample preparation was accomplished through a protein precipitation extraction process. Chromatographic resolution of flavokawain B and the IS was achieved on an Agilent XDB-C18 column (2.1 mm × 100 mm, 1.8 μm) using a gradient mobile phase comprising 0.1% formic acid in water and acetonitrile delivered at a flow rate of 0...
September 20, 2018: Biomedical Chromatography: BMC
Yongqing Wen, Xuemei Yuan, Feng Qin, Longshan Zhao, Zhili Xiong
Determination of amino acids in biofluids was a challenging task because of difficulties derived from their high polarity and matrix interference. A simple, reliable and high-throughput hydrophilic interaction UHPLC-MS/MS method was developed and validated for the rapid simultaneous determination of 19 free amino acids in rat plasma and urine samples in this paper. Hydrophilic method with Waters ACQUITY UPLC BEH Amide column(100×2.1mm,1.7μm) was used with a gradient mobile phase system of acetonitrile and water both containing 0...
September 20, 2018: Biomedical Chromatography: BMC
Marija Čarapić, Katarina Nikolic, Bojan Marković, Miloš Petković, Milena Pavlovic, Danica Agbaba
Separation and characterization of the unknown degradation product of second generation antipsychotic drug ziprasidone was essential for defining genotoxic potential of the compound. The aim of this study was to develop simple UHPLC method coupled with tandem mass spectrometry (MS/MS) for chemical characterization of unknown degradant, separation and quantification of ziprasidone and its five main impurities (I-V) in raw material and pharmaceuticals. Chromatographic conditions were optimized by experimental design...
September 14, 2018: Biomedical Chromatography: BMC
Fawzy Elbarbry, Van Nguyen, Alar Mirka, Heather Zwickey, Richard Rosenbaum
A simple, accurate, and reproducible HPLC-UV method has been developed and validated for the quantification of levodopa (L-Dopa) in human plasma. The method involves a simple protein precipitation procedure to extract both L-Dopa and methyldopa, the internal standard. The chromatographic analysis was achieved on a Shimadzu LC 20A HPLC system equipped with a Zorbax Eclipse XDB C18 column and an isocratic mobile phase consisted of 20 mM KH2 PO4 (pH 2.5) and methanol (95:5, v/v) run at a flow rate of 1 ml/min...
September 11, 2018: Biomedical Chromatography: BMC
Edoardo Napoli, Davide Gentile, Giuseppe Ruberto
No abstract text is available yet for this article.
September 7, 2018: Biomedical Chromatography: BMC
Jianbo Chen, Chen Lixue, Meijia Li, Yinshi Sun
In this study, a sensitive hydrophilic interaction liquid chromatography (HILIC) method was developed to determine pseudojervine (PJV), veratrosine (VTS), jervine (JV), veratramine (VTM), veramarine (VA) and veratroylzygadenine (VTG) in rat plasma. Separations were carried out using an LC-MS/MS with Chrom Matrix HP amide column (5 m, 10 cm ×3.0 mm I.D.). The mobile phases were composed of A (0.01 mM formic acid) and B (acetonitrile). Good linearity was found for all analytes (R2 > 0.995) in the concentration ranging from 5 to 1000 ug/L with LLOQ at 5 ug/L for VTM and VTS; from 1 to 1000 ug/L with LLOQ at 1 ug/L for PJV, JV, VA and VTG...
September 6, 2018: Biomedical Chromatography: BMC
Pengfei Xu, Yonggui Song, Bingwei Feng, Qiang Zeng, Baixi Shan, Kuangyi Liu, Dan Su
Keke capsule as a traditional Chinese medicine formulation is used to relieve a cough, analgesia and reduce bronchial asthma. The multi-components are absorbed into blood and brain after oral administration of Keke capsule, with no systematic investigation till now. A reliable and rapid UPLC-QTOF-MSE combined with data processing software platform was used to characterize the components of Keke capsule and simultaneously identify bioactive components in blood and brain tissues in rat after oral administration, respectively...
September 4, 2018: Biomedical Chromatography: BMC
Pavan Kumar Prathipati, Subhra Mandal, Christopher J Destache
A sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for quantification of bictegravir in human plasma. A small volume of only 50 μL aliquot of plasma was precipitated with acetonitrile containing an internal standard (IS). Chromatographic separation was performed on Kinetex EVO C18 column, 50 × 3.0 mm, 5 μm using an isocratic mobile phase containing 80:20 acetonitrile/water with 0.1% formic acid. A mass spectrometer operated in ESI positive multiple reaction monitoring mode using the m/z 450...
September 4, 2018: Biomedical Chromatography: BMC
Yue Geng, Linjie Jiang, Hongxin Jiang, Lu Wang, Yi Peng, Ce Wang, Xiaomeng Shi, Jing Gu, Yuehua Wang, Jiachao Zhu, Lihong Dai, Yaping Xu, Xiaowei Liu
A quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction approach and gas chromatography/tandem mass spectrometry with programmed temperature vaporization sampling technology were used to determine fungicide quintozene and its hazardous impurity hexachlorobenzene (HCB) in Panax notoginseng root, which is commonly used as a rare traditional Chinese medicine worldwide. The mean recoveries were in the range of 94-125% and 84-119% for quintozene and HCB with relative standard deviations of 6.2-16...
September 3, 2018: Biomedical Chromatography: BMC
Justin Beltz, Annalise Pfaff, Nuran Ercal
Tiopronin, formally 2-mercaptopropionylglycine (MPG), is currently prescribed to treat cystinuria and rheumatoid arthritis, and its antioxidant properties have led to its investigation as a treatment for cataracts, a condition in which oxidative stress is strongly implicated. To study its accumulation in the eye, a reliable, isocratic HPLC method was developed for the determination of MPG and its primary metabolite 2-mercaptopropionic acid (MPA) in plasma and relevant ocular tissues. This method utilizes pre-column derivatization and fluorescence detection...
September 3, 2018: Biomedical Chromatography: BMC
Yufei Feng, Zhongxu Jing, Yongji Li, Shaowa Lv, Weinan Li, Guofeng Cai, Danqi Yang, Yanhong Wang
SHLI has been used in China for over 30 years as an effective and widely used Chinese herbal prescription to treat acute respiratory infectious. SHLI has, however, caused many severe anaphylactoid reactions. It is important to identify the potential anaphylactoid components of SHLI. Spectrum-effect relationships were used to explore potentially anaphylactoid components. Based on the original herbal formula, honeysuckle, Fructus forsythiae and Radix scutellariae extracts were prepared and combined in appropriate proportions...
August 31, 2018: Biomedical Chromatography: BMC
Wei Fan, Jiebin Hou, Weiye Zhu, Shuyue Zhang, Keda Shao, Feiyu Quan, Wei Chen
Kidney stone disease is a worldwide metabolism associated disorder with a high incidence of renal dysfunction. However, the effective methods to prevent crystalline nephropathy are still lacking with the absence of aetiological research. Shen'an (SA) capsules are prepared from Chinese medicinal compounds and are considered a promising treatment for the prevention of crystal-induced renal injury. In this study, twenty-four mice were randomly divided into 4 groups including the saline, oxalate, SA-treated (via preventive administration) and SA only groups...
August 23, 2018: Biomedical Chromatography: BMC
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