We have located links that may give you full text access.
Tricine co-ligand improved the efficacy of 99m Tc-HYNIC-(Ser) 3 -J18 peptide for targeting and imaging of non-small-cell lung cancer.
Biomedicine & Pharmacotherapy 2018 August
The early diagnosis of non-small cell lung cancer (NSCLC) is important for increasing survival rate and improving quality life of patients. The aim of this study was to investigate 99m Tc-(tricine)-HYNIC-(Ser)3 -J18 for targeting and imaging of NSCLC in A-549 xenografted nude mice. The (Ser)3 -J18 peptide was conjugated with HYNIC and labeled with 99m Tc using tricine as a co-ligand. The radiolabeled peptide was evaluated for its radiochemical purity, stability, receptor binding and internalization in vitro. The future experiments were performed for tumor targeting and imaging in A-549 tumor-bearing mice. 99m Tc-(tricine)-HYNIC-(Ser)3 -J18 was obtained at high labeling efficiency at room temperature and favorable stability in saline and human plasma. At the cellular level, the radiolabeled peptide specifically bond to A-549 cells with a KD 4.1 ± 1.3 nM. Biodistribution study revealed tumor to blood and tumor to muscle ratios were about 3.12 and 5.63 respectively after 2 h injection of radiolabeled peptide. These ratios were significantly decreased by co-injection of excess non-labeled peptide in mice. This radiolabeled peptide selectively targeted to NSCLC tumor and exhibited a high target uptake combined with acceptable low background activity for tumor imaging in mice. The results of this study and its comparison with another study showed that 99m Tc-(tricine)-HYNIC-(Ser)3 -J18 is better than previously reported radiolabeled peptide as 99m Tc-(EDDA/tricine)-HYNIC-(Ser)3 -J18 for NSCLC targeting and imaging.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app