Age-Specific 18 F-FDG Image Processing Pipelines and Analysis Are Essential for Individual Mapping of Seizure Foci in Pediatric Patients with Intractable Epilepsy.

18 F-FDG PET is an important tool for the presurgical assessment of children with drug-resistant epilepsy. Standard assessment is performed visually and is often subjective and highly user-dependent. Voxelwise statistics can be used to remove user-dependent biases by automatically identifying areas of significant hypo- or hypermetabolism associated with the epileptogenic area. In the clinical setting, this analysis is performed using commercially available software. These software packages suffer from two main limitations when applied to pediatric PET data: pediatric scans are spatially normalized to an adult standard template, and statistical comparisons use an adult control dataset. The aim of this work was to provide a reliable observer-independent pipeline for the analysis of pediatric 18 F-FDG PET scans, as part of presurgical planning in epilepsy. Methods: A pseudocontrol dataset (19 subjects 6-9 y old, and 93 subjects 10-20 y old) was used to create two age-specific 18 F-FDG PET pediatric templates in standard pediatric space. The 18 F-FDG PET scans of 46 epilepsy patients (16 patients 6-9 y old, and 30 patients 10-17 y old) were retrospectively collated and analyzed using voxelwise statistics. This procedure was implemented with the standard pipeline available in the commercial software Scenium and an in-house Statistical Parametric Mapping, version 8 (SPM8), pipeline (including age-specific pediatric templates and reference database). A κ-test was used to assess the level of agreement between the findings of voxelwise analyses and the clinical diagnosis of each patient. The SPM8 pipeline was further validated using postsurgical seizure-free patients. Results: Improved agreement with the clinical diagnosis was reported using SPM8, in terms of focus localization, especially for the younger patient group: κ = 0.489 for Scenium versus 0.826 for SPM. The proposed pipeline also showed a sensitivity of about 70% in both age ranges for the localization of hypometabolic areas on pediatric 18 F-FDG PET scans in postsurgical seizure-free patients. Conclusion: We showed that by creating age-specific templates and using pediatric control databases, our pipeline provides an accurate and sensitive semiquantitative method for assessing the 18 F-FDG PET scans of patients under 18 y old.