There is no difference between sequences of HIV-1 infected patients with stable clinical status and HIV-1 reference sequence.

BACKGROUND: The rate of human immunodeficiency virus type 1 (HIV-1) infection in Iran has increased dramatically in the past few years. HIV-1 genome sequences are pivotal for large-scale studies of inter- and intra-host evolution. To understand the molecular difference between reference HIV-1 isolate and two HIV-1 infected patients in Iran, we conducted this study to analyze some genome segments of Iranian HIV-1 isolates.

METHODS: Two HIV-1-infected individuals who were under antiretroviral therapy (ARV) for 8 years with stable clinical status were enrolled. Gag-Pol genome sequences (4500 nt) obtained from patient's plasma samples were used for constructing phylogenetic tree and analyzing similarity plotty.

RESULTS: Both isolates belonged to CRF35_AD subtype. One of them had drug resistance. Our patient's HIV genome and protein isolates showed no clear difference with genome and protein sequences of reference sequence.

CONCLUSIONS: our patient's stable clinical status had no connection to genome sequence; it could be owing to immunological factors or other patient's mode which are still unknown.