A label-free platform for dopamine biosensing.

Jesús Navarro, Javier Galbán, Susana de Marcos

Bioanalysis 2018 January

AIM: This work presents a label-free platform for dopamine (DA) monitoring based on the spectroscopic properties of laccase.

RESULTS: Working in batch mode, DA ranging from 25 to 250 μM, can be determined without the interference of norepinephrine and epinephrine. Laccase immobilized in a polyacrylamide film is the basis of a platform for the label-free determination of DA. The linear range goes from 100 to 900 μM with an RSD of 5.3% and a film lifetime of more than 30 measurements. The biosensors also permit the DA + epinephrine + norepinephrine determination.

CONCLUSION: The method permits the determination of DA and the total concentration of the three neurotransmitters, and could be used for DA monitoring in urine samples.

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