Read by QxMD icon Read


Akram Khodabandehloo, David Da Yong Chen
Protein aggregation is a common biological phenomenon which is responsible for degenerative diseases and is problematic in the pharmaceutical industry. According to the rules provided by regulatory agencies, industry is supposed to assess the product quality regarding the presence of subvisible particles. Also, they should evaluate the technologies that are used to measure these particles. Therefore, US FDA and industry have been looking for methods capable of accurately characterizing the protein products...
January 18, 2017: Bioanalysis
Jianxia Shi, Jing Man Wong, Ji Ma, Tiffany Dickerson, Colin Hall, Dan A Rock, Timothy J Carlson
AIM: Immobilized metal ion affinity chromatography is widely employed for purifying polyhistidine-tagged recombinant proteins from cell lysates. The technique can be applied for quantification of therapeutic proteins in biological matrices by LC-MS/MS. RESULTS: A protein reagent-free workflow was developed for quantifying polyhistidine-tagged proteins by LC-MS/MS. The workflow includes target protein enrichment by immobilized metal ion affinity chromatography, on-bead trypsin digestion and quantification of signature peptides by LC-MS/MS...
January 18, 2017: Bioanalysis
Álvaro Luque-Uría, Anunciación Espinosa-Mansilla, Isabel Durán-Merás
AIM: An HPLC method for the determination of 2-hydroxyfluorene (2-OHF), various hydroxyphenanthrene metabolites (1-, 2-, 3-, 4- and 9-hydroxyphenanthrene, OHPhs), 1-hydroxypyrene (1-OHPy) and 3-hydroxybenzo[a]pyrene (3-OHB[a]Py) in human urine, has been developed using fast scanning fluorimetric detection and gradient elution mode. MATERIALS & METHODS: All reagents were of analytical grade. Standard solutions were prepared separately, by exact weighing or dilution with ultrapure acetonitrile, and were stored at 4 ºC in darkness...
January 18, 2017: Bioanalysis
Guy Tremblay
No abstract text is available yet for this article.
January 13, 2017: Bioanalysis
Pranov Ramana, Ward Herman, Charlotte Hiroux, Erwin Adams, Patrick Augustijns, Ann Van Schepdael
AIM: In this paper, we developed a method to immobilize human flavin-containing monooxygenase-3 (hFMO3) using glutaraldehyde as a cross-linker onto amino-functionalized magnetic nanoparticles. MATERIALS & METHODS: All the analyses were done using capillary electrophoresis coupled with a diode array detector using clozapine as a substrate. RESULTS: The apparent Km with clozapine as substrate and inhibition of hFMO3 by methimazole were explored for immobilized hFMO3 and were found to be comparable to literature values...
January 13, 2017: Bioanalysis
Jiaqing Chen, Fengguo Xu
No abstract text is available yet for this article.
January 13, 2017: Bioanalysis
Joseph D Manna, Samantha J Richardson, Mehran F Moghaddam
AIM: Increasing numbers of compounds requiring stability data means highly optimized methods capable of rapid turnaround are desirable during early discovery. Materials and methods/results: An advanced, generic analytical workflow for metabolic stability has been developed that utilizing ballistic gradient LC (sub 1 min run times), exact mass TOF-MS (Waters Xevo-G2-XS Q-TOF) and automated data processing (Waters UNIFI software) allowed for rapid integration and interpretation of all data produced, eliminating the need for method development and manual processing...
January 11, 2017: Bioanalysis
Chengsen Zhang, Brandon J Bills, Nicholas E Manicke
No abstract text is available yet for this article.
January 10, 2017: Bioanalysis
Tina Panchal, Neil Spooner, Matthew Barfield
AIM: The quality of quantitative analytical measurements is dependent on the quality of the sample collected, and dried blood spots (DBS) are no exception. As the use of DBS has matured into late-stage clinical drug-development studies, it has become apparent that a simple and straightforward approach in a controlled single-site, first-time-into-human clinic, does not always translate into multicenter clinical studies. Using synthetic blood, a method of training and assessing clinical laboratory staff has been developed to ensure the quality of sampling...
January 2017: Bioanalysis
Xinyu Wang, Jing-Lin Gao, Chaohui Du, Jing An, MengJiao Li, Haiyan Ma, Lina Zhang, Ye Jiang
AIM: People today have a stronger interest in the risk of biosafety in clinical bioanalysis. A safe, simple, effective method of preparation is needed urgently. METHODOLOGY/RESULTS: To improve biosafety of clinical analysis, we used antiviral drugs of adefovir and tenofovir as model drugs and developed a safe pretreatment method combining sealing technique with direct injection technique. The inter- and intraday precision (RSD %) of the method were <4%, and the extraction recoveries ranged from 99...
January 2017: Bioanalysis
Shinji Kobuchi, Megumi Matsuno, Momoko Kawamoto, Naoto Kojima, Yukako Ito, Masayuki Yamashita, Toshiyuki Sakaeda
AIM: Luseogliflozin is a novel sodium-dependent glucose cotransporter-2 inhibitor for the treatment of Type 2 diabetes mellitus. To assist pharmacokinetic and toxicodynamic studies, a rapid LC-MS/MS method were developed and validated for the quantitation of luseogliflozin in rat plasma. RESULTS: Sample preparation was carried out using simplified protein precipitation and liquid-liquid extraction procedures, and the run time was only 4 min. Extraction recovery was 92...
January 2017: Bioanalysis
Daniel Tang, Dafang Zhong, Kelly Dong
The fourth China Bioanalysis Forum annual conference, co-organized with the Nanjing International Drug Metabolism Conference, was successfully held in Nanjing, China, between 24-26 June 2016. The theme of the conference was 'how to conduct regulated bioanalysis under China Food and Drug Administration (CFDA) regulations'. In addition, several hot topics including bioanalytical challenges, solutions for biomarkers, antidrug antibody (antibody-drug conjugates) and biologics were discussed. This Conference Report summarizes the major discussion topics from the conference...
January 2017: Bioanalysis
Jana Zemenová, David Sýkora, Lenka Maletínská, Jaroslav Kuneš
A number of novel lipopeptides have been studied for their possible therapeutic potential. These studies should be supported by the appropriate analytical tools not only for novel potential drugs but also for their metabolites, precursors and side products. Lipopeptides have specific physicochemical properties that make them successful in medical applications. However, there are some difficulties with their qualitative and quantitative analyses in biological samples. Therefore, reliable, sensitive and robust analytical methods are in high demand...
January 2017: Bioanalysis
Yuan-Qing Xia, Eugene Ciccimaro, Naiyu Zheng, Mingshe Zhu
AIM: It is challenging to develop a multiple reaction monitoring (MRM) method for some disulfide-bonded peptides with inefficient collision-induced dissociation fragmentation. This study describes a new methodology using differential mobility spectrometry (DMS) combined with multiple ion monitoring (MIM) to enhance bioanalytical sensitivity for sunflower trypsin inhibitor. RESULTS: By combining DMS with MIM to monitor the intact precursor ion in Q1 and Q3 MS analyzers, a lower limit of quantitation at 0...
January 2017: Bioanalysis
Yanhua Chen, Zeper Abliz
No abstract text is available yet for this article.
January 2017: Bioanalysis
Jia Duo, JoAnne Bruno, Steven Piccoli, Binodh DeSilva, Yan J Zhang
AIM: Ligand-binding assay (LBA) reagent labeling may change the binding characteristics of the reagent to its target and degrade its performance in LBAs. RESULTS: A surface plasmon resonance (SPR) biosensor was used to evaluate the impact of the biotin labeling process on reagent-binding kinetics and affinity for a specific target. The SPR results demonstrate that the biotin molar challenge ratio affects both association and dissociation rates for the labeled reagent binding to its target...
January 2017: Bioanalysis
Sankeetha Nadarajah
Welcome to volume 9 of Bioanalysis and Happy New Year to all of our readers. We would like to take the opportunity to look back 2016, which was another great year for us. We thank all our authors, readers and reviewers, as well as our Editorial Board members for their continued support. We very much look forward to working with everyone in 2017.
January 2017: Bioanalysis
Raúl González-Domínguez, Ana Sayago, Ángeles Fernández-Recamales
Metabolomics based on direct mass spectrometry (MS) analysis, either by direct infusion or flow injection of crude sample extracts, shows a great potential for metabolic fingerprinting because of its high-throughput screening capability, wide metabolite coverage and reduced time of analysis. Considering that numerous metabolic pathways are significantly perturbed during the initiation and progression of diseases, these metabolomic tools can be used to get a deeper understanding about disease pathogenesis and discover potential biomarkers for early diagnosis...
January 2017: Bioanalysis
Georgios Moros, Anastasia Chrysovalantou Chatziioannou, Helen G Gika, Nikolaos Raikos, Georgios Theodoridis
AIM: Metabolomics applications represent an emerging field where significant efforts are directed. Derivatization consists prerequisite for GC-MS metabolomics analysis. METHODS: Common silylation agents were tested for the derivatization of blood plasma. Optimization of methoxyamination and silylation reactions was performed on a mixture of reference standards, consisting of 46 different metabolites. Stability of derivatized metabolites was tested at 4°C. RESULTS: Optimum results were achieved using N-methyl-N-(trimethylsilyl)trifluoroacetamide...
January 2017: Bioanalysis
C Virgiliou, L Valianou, M Witting, F Moritz, C Fotakis, P Zoumpoulakis, A C Chatziioannou, L Lazaros, G Makrydimas, K Chatzimeletiou, N Raikos, G A Theodoridis
AIM: Till now there is very limited knowledge on the molecular content of coelomic fluid and cells. This study presents the first attempt to elucidate the metabolic profile of such samples. METHODOLOGY: Samples were collected via coelocentesis from 41 women during the first trimester of gestation. Metabolic content was assessed using four different analytical platforms. For targeted analysis a hydrophilic interaction chromatography ultra high performance LC-MS/MS method was applied...
January 2017: Bioanalysis
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"