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Bioanalysis

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https://www.readbyqxmd.com/read/28644046/paper-based-point-of-care-testing-in-disease-diagnostics
#1
Leila Syedmoradi, Frank A Gomez
No abstract text is available yet for this article.
June 23, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28644045/method-development-of-glycoprotein-biomarkers-for-cancers
#2
Shuang Yang, Perry G Wang
No abstract text is available yet for this article.
June 23, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28627276/comprehensive-graphical-presentation-of-data-from-incurred-sample-reanalysis
#3
Piotr J Rudzki, Przemysław Biecek, Michał Kaza
AIM: Incurred sample reanalysis (ISR) contributes to the reliability of pharmacokinetic studies. Despite regulatory guidelines having adopted ISR methodology, graphical presentation of data has been overlooked. MATERIALS & METHODS: Different graphs were tested for datasets including limited, standard and large numbers of ISR pairs. The datasets covered both passed and failed cases. RESULTS: We have developed a combination of complementary plots enabling the visual inspection of ISR data quality: %difference versus mean concentration and cumulative ISR plot...
June 19, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28627228/affinity-extraction-lc-ms-for-small-molecule-drug-quantification-considerations-challenges-and-potential-applications
#4
John T Mehl
No abstract text is available yet for this article.
June 19, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28627223/advances-in-derivatization-approaches-for-increasing-the-sensitivity-of-small-molecules
#5
Makoto Niwa
No abstract text is available yet for this article.
June 19, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28627218/performance-evaluation-of-three-platforms-with-ultrasensitive-ligand-binding-assay-potential
#6
Kyra Juliana Cowan, Albert Geiger, Hans Hornauer, Robyn Rourick, Patricia Yanira Siguenza
AIM: We evaluated three immunoassay-based technologies and their biomarker kits, by determining precision, parallelism and detectability of analytes of interest. MATERIALS & METHODS: We compared ultrasensitive assays for three biomarkers: interleukins IL-6, IL-13 and IL-17A using kits obtained from Roche (IMPACT platform - proprietary platform), Singulex (Erenna(®)) and Quanterix (Simoa(™)). We defined the true LLOD as the LLOQs, and provided disease-specific parallelism results and detectability levels for endogenous analyte, which were good across platforms, though they varied from analyte to analyte...
June 19, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28617093/micro-spe-in-pipette-tips-as-a-tool-for-analysis-of-small-molecule-drugs-in-serum
#7
Hana Vlčková, Veronika Pilařová, Ondřej Novák, Petr Solich, Lucie Nováková
AIM: Micro-SPE in pipette tips (μ-SPE-PT) with particle sorbent has never been used in small-molecule drug analysis. Methodology & results: μ-SPE-PT was used for the extraction of statins from biological materials followed by UHPLC-MS/MS. The commercial and homemade μ-SPE-PT tips filled with particle sorbent were compared. While the homemade tips enabled direct serum sample loading into the sorbent, protein precipitation (PP) had to be implemented before μ-SPE-PT procedure using commercial tips...
June 15, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28617069/simultaneous-determination-of-six-tyrosine-kinase-inhibitors-in-human-plasma-using-hplc-q-orbitrap-mass-spectrometry
#8
Mao-Wei Ni, Jie Zhou, Hui Li, Wei Chen, Han-Zhou Mou, Zhi-Guo Zheng
AIM: Gefitinib, erlotinib, icotinib, crizotinib, lapatinib and apatinib are targeted cancer therapy agents acting through inhibition of tyrosine kinase. Method for quantifying these six drugs in human plasma of patients was required. MATERIALS & METHODS: An HPLC-Q-Orbitrap method (based on HPLC-MS/MS) was developed and validated for the simultaneous detection and quantitation of six tyrosine kinase inhibitors in human plasma. Sample was extracted by liquid-liquid extraction (ethyl acetate: tert-Butyl methyl ether, 1:1 v/v)...
June 15, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28617037/quantitative-determination-of-a-sirna-ad00370-in-rat-plasma-using-peptide-nucleic-acid-probe-and-hplc-with-fluorescence-detection
#9
Qingguo Tian, Juan Rogness, Min Meng, Zheng Li
AIM: Toxicokinetic and pharmacokinetic studies of therapeutic oligonucleotides require validated bioanalytical methods for sensitive and specific quantification of oligonucleotide drug candidates in biological samples. RESULTS: A peptide nucleic acid (PNA) hybridization-based HPLC-fluorescence assay was developed and validated for quantification of Arrowhead Pharmaceuticals' proprietary siRNA in rat plasma samples via hybridization and anion-exchange-HPLC (AEX-HPLC) with fluorescence detection...
June 15, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28617036/an-lc-ms-ms-method-to-determine-vancomycin-in-plasma-total-and-unbound-urine-and-renal-replacement-therapy-effluent
#10
Suzanne L Parker, Yarmarly C Guerra Valero, Jenny L Ordóñez Mejia, Claire Roger, Jeffrey Lipman, Jason A Roberts, Steven C Wallis
AIM: Critical illness and medical interventions, such as renal replacement therapy, can cause changes to vancomycin pharmacokinetics and lead to suboptimal dosing. To comprehensively characterize vancomycin pharmacokinetic a method must measure vancomycin in a range of clinical matrices. RESULTS: A LC-MS/MS method was developed using hydrophilic interaction liquid chromatography and microsample volumes, where possible. For all matrices, the linear concentration range was 1-100 μg/ml, interassay accuracy and precision was within 15%, and recovery above 80%...
June 15, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28617033/a-uplc-ms-ms-method-for-quantification-of-5%C3%AE-androst-3%C3%AE-5-6%C3%AE-triol-in-human-plasma-development-validation-and-its-application-in-clinical-pharmacokinetic-study
#11
Zhenlei Wang, Huanhuan Wang, Pei Hu, Ji Jiang, Qian Zhao
AIM: 5α-androst-3β,5,6β-triol is a novel ischemic stroke drug under clinical development. The objective of this study was to develop and validate a simple ultraperformance liquid chromatography tandem mass spectrometry method for 5α-androst-3β,5,6β-triol in human plasma and its application in clinical pharmacokinetic study. Methodology & results: After being pretreated using an automatized solid-phase extraction procedure, plasma sample was separated on a Waters(®) Acquity™ BEH C18 column (2...
June 15, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28534642/corrigendum
#12
(no author information available yet)
No abstract text is available yet for this article.
May 23, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28524734/capillary-microsampling-in-nonclinical-safety-assessment-practical-sampling-and-bioanalysis-from-a-cro-perspective
#13
David Coleman, Graeme Smith, Rachel Lawrence, Deborah McManus, Sunetha Diaram, Joanna Edwards
Microsampling has the 3R benefits of refining blood collection techniques while reducing the number of animals required for rodent safety assessment studies. There are significant scientific benefits of correlating study findings with systemic exposure and consequently, there is an industry drive to utilize microsampling in regulatory toxicology studies. This article will focus on capillary microsampling and will discuss the initial considerations before using capillary microsampling, study design and some practicalities of sample collection...
May 19, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28520467/challenges-in-metabolomics-based-disease-molecular-classification-an-analytical-perspective
#14
Yiqiao Gao, Quan Liu, Fengguo Xu
No abstract text is available yet for this article.
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28520466/development-and-validation-of-a-highly-sensitive-gradient-chiral-separation-of-pramipexole-in-human-plasma-by-lc-ms-ms
#15
Marco Michi, Massimo Breda, Justin Keane Belardi, Steven Dworetzky, Lara Solazzo
AIM: Development of a high-sensitivity chiral LC-MS/MS method was required to evaluate a combination of pramipexole (S-PPX) and its enantiomer dexpramipexole (R-PPX) in a proposed clinical trial. The previously available methods suffered from low sensitivity for the (S)-enantiomer in the presence of the more abundant (R)-enantiomer. Based on the projected dosing regimen in the clinical trial, a 5000-fold improvement in sensitivity was required for the (S)-enantiomer. METHODOLOGY: Spiked human plasma samples were extracted by liquid-liquid extraction using ethyl acetate and injected onto a CHIRALPAK ID column under pH gradient conditions...
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28520462/activity-based-proteomics-in-bioanalysis-past-present-and-future
#16
Rituraj Dubey, Ravi Bhushan
No abstract text is available yet for this article.
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28520459/feedback-from-the-ebf-focus-workshop-bringing-assay-validation-and-analysis-of-biomarkers-into-practice
#17
Philip Timmerman, Marianne Scheel Fjording, John Allinson, Cecilia Arfvidsson, Begoña Barroso, Ulf Diczfalusy, Adrian Freeman, Elizabeth Hickford, Hamza Kandousi, Sidath Katugampola, Ulrich Kunz, Robert Nelson, John Smeraglia
European Bioanalysis Forum, Lisbon, Portugal, 9-10 June 2016 At the recent European Bioanalysis Forum's Focus Workshop 'Bringing Assay Validation and Analysis of Biomarkers into Practice', the discussion on best practice for biomarker assay validation continued. Both the presentations and the adjacent panel discussions yielded valuable food for thought for the broader bioanalytical community. The present conference report summarizes the essence from these discussions and from the proposals or conclusions made by all delegates on how to increase the necessary connectivity of the stakeholders involved in the bioanalysis of biomarkers...
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28520457/measuring-and-using-free-drug-concentrations-has-there-been-real-progress
#18
Florin Marcel Musteata
No abstract text is available yet for this article.
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28520456/development-and-validation-of-a-micellar-liquid-chromatographic-method-to-determine-three-antitumorals-in-plasma
#19
Josep Esteve Romero, Jaume Albiol Chiva, Juan Peris-Vicente, Enrique Ochoa-Aranda
AIM: A micellar liquid chromatographic method to determine several anticancer drugs (pazopanib, dabrafenib and regorafenib) in plasma was developed and validated by the guidelines of the EMA. EXPERIMENTAL: Plasma samples were directly injected, after a 1/5-dilution in a micellar solution. The drugs were resolved in <18 min using a C18 column. The mobile phase was an aqueous solution of 0.12 M SDS - 2% 1-pentanol, buffered at pH 7. The detection was performed by absorbance at 260 nm...
May 18, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28520455/quantitative-determination-of-avi-7100-radavirsen-a-phosphorodiamidate-morpholino-oligomer-pmoplus-%C3%A2-in-human-plasma-using-lc-ms-ms
#20
Min Meng, Jianbo Zhang, Aihua Liu, Scott Reuschel, Pete Sazani, Michael Wong
AIM: AVI-7100 (Radavirsen) is a 20-mer phosphorodiamidate morpholino oligomer (PMOplus®) for the treatment of influenza. Results/methodology: An automated solid-phase extraction method was used to extract plasma samples (200 μl). The extracts were analyzed using liquid chromatography coupled with tandem mass spectrometry under the positive ionization mode. This method was fully validated over the calibration curve range of 5.00-1000 ng/ml. The between-run precision and accuracy ranged from 0...
May 18, 2017: Bioanalysis
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