Read by QxMD icon Read


Yue Zhao, Guowen Liu, Suk Kwok, Barry R Jones, Jane Liu, David Marchisin, Philip E Joyce, Jon Peterson, Jim X Shen
AIM: Recombinant FGF21 analogs are under wide ranging investigations as a potential therapeutic agent for Type 2 diabetes, as well as other metabolic disorders. The endogenous FGF21 is often used as a surrogate pharmacodynamic(PD) biomarker to assess drug efficacy and safety. Results/methodology: Immunocapture was performed using a monoclonal antibody which had been generated to bind to specific domain of native FGF21 as the capture reagent. After immunocapture, enzymatic digestion was performed and a native FGF21-specific tryptic peptide was monitored using LC-MS/MS by selective reaction monitoring...
December 14, 2017: Bioanalysis
Adrian Arendowski, Joanna Nizioł, Krzysztof Ossoliński, Anna Ossolińska, Tadeusz Ossoliński, Zygmunt Dobrowolski, Tomasz Ruman
AIM: Renal cell carcinoma is a very aggressive and often fatal disease for which there are no specific biomarkers found to date. The purpose of work was to find substances that differentiate the cancerous and healthy tissue by using laser desorption/ionization MS imaging combined with silver nanoparticle-enhanced target. RESULTS: Ion images and comparative analysis of spectra revealed differences in intensities for several metabolites, for which their biochemical properties were discussed...
December 14, 2017: Bioanalysis
Nan Liu, Fengjiao Zheng, Xiaoling Zheng
AIM: In order to assist and support early diagnosis of acute myocardial infarction (AMI), suspension array technology was established for multiplexed, rapid and accurate measurement of AMI biomarkers in serum samples. METHODOLOGY/RESULTS: It was developed by coating AMI biomarkers on distinguishable microbeads and competing with free biomarkers for complementary antibodies. The limits of detection of three AMI biomarkers were 2.5- to 50-times lower than that of the previous methods and the working ranges were four to five orders of magnitude...
December 14, 2017: Bioanalysis
Amandine Dispas, Hugues Jambo, Sébastien André, Eva Tyteca, Philippe Hubert
During the last years, chemistry was involved in the worldwide effort toward environmental problems leading to the birth of green chemistry. In this context, green analytical tools were developed as modern Supercritical Fluid Chromatography in the field of separative techniques. This chromatographic technique knew resurgence a few years ago, thanks to its high efficiency, fastness and robustness of new generation equipment. These advantages and its easy hyphenation to MS fulfill the requirements of bioanalysis regarding separation capacity and high throughput...
December 13, 2017: Bioanalysis
Priyanka A Shah, Pranav S Shrivastav
No abstract text is available yet for this article.
December 13, 2017: Bioanalysis
Maryam Khoubnasabjafari, Elaheh Rahimpour, Abolghasem Jouyban
No abstract text is available yet for this article.
December 13, 2017: Bioanalysis
Katja Heinig, Enric Bertran, Julian Potter, Daniela Fraier
There are many ocular diseases still presenting unmet medical needs. Therefore, new ophthalmologic drugs are being developed. Bioanalysis of eye compartments (along with plasma and other tissues) is important to determine exposure of the target organ to the drug and to help interpret local pharmacological or toxic effects. This review article identifies several challenges that occur within ocular bioanalysis. They include sample collection and preparation, analytical issues, sourcing control matrix, data interpretation and regulatory requirements...
December 5, 2017: Bioanalysis
Jelena Maksić, Ana Stajić, Miroslav Knežević, Bojana Dačić, Krnjaja Biljana Jančić-Stojanović, Mirjana Medenica
AIM: The objective of the study was development of hydrophilic interaction liquid chromatography-ESI/MS/MS method for the determination of olopatadine in tear matrix. MATERIALS & METHODS: Separation was performed on Acquity BEH amide column (2.1 mm × 100 mm, 1.7 μm). The mobile phase was consisted of 0.1% formic acid in water and acetonitrile. Mianserin hydrochloride was implemented as an internal standard. The artificial tear fluid was used as matrix. The tear samples were collected using Schirmer test strips...
December 5, 2017: Bioanalysis
Shane R Needham
No abstract text is available yet for this article.
December 5, 2017: Bioanalysis
Lieve Dillen, Luc Sips, Tony Greway, Tom Verhaeghe
AIM: Imetelstat, a 13-mer oligonucleotide with a lipid tail is being evaluated for treating hematologic myeloid malignancies. This report describes the development of extraction and quantification methods for imetelstat. Methodology & results: Imetelstat was extracted using SPE (rat plasma) or by hybridization using a biotinylated capture probe (human plasma) and was quantified by LC-MS/MS. Calibration curves were established (0.1-50 μg/ml). Stability of imetelstat in plasma was demonstrated...
December 5, 2017: Bioanalysis
Yingfei Yan, Zhifeng Du, Hongliang Jiang
No abstract text is available yet for this article.
December 5, 2017: Bioanalysis
Wenkui Li, Jiawei Gong, Chuan Wang, Zhongping John Lin, Jimmy Flarakos
AIM: To develop and validate an inductively coupled plasma-mass spectrometry method for quantitative bioanalysis of aluminum (Al) in monkey serum in support of a GLP TOX study with alhydrogel-formulated drug candidate. METHODS & RESULTS: The method was linear over a dynamic range of 10-1000 ng/ml using a 50-μl sample volume. The intra-/inter-run precision (%CV) of the quality control sample results were ≤7.9% (CV) and the accuracy (%bias) within ±11.0% across all quality control concentrations evaluated...
November 24, 2017: Bioanalysis
Wanlong Zhou, Shuang Yang, Perry G Wang
No abstract text is available yet for this article.
November 24, 2017: Bioanalysis
Mariana Millan Fachi, Letícia Paula Leonart, Flávia Lada Degaut Pontes, Raquel de Oliveira Vilhena, Letícia Bonancio Cerqueira, Roberto Pontarolo
Type 2 diabetes mellitus, a disease which prevalence has been progressively increasing worldwide, is characterized by chronic hyperglycemia resulting from the combination of inappropriate insulin secretion and/or resistance to insulin action. If left uncontrolled, diabetes is associated with complications such as dysfunction and failure of various organs, and even premature death. Along with lifestyle-modification strategies, several classes of oral antidiabetic agents can be employed for glycemic control. Thus, therapeutic drug monitoring of these drugs is essential to maintain appropriate treatment...
November 24, 2017: Bioanalysis
Petia Shipkova, Eugene Ciccimaro, Timothy V Olah
No abstract text is available yet for this article.
November 24, 2017: Bioanalysis
Andrew Tl Wotherspoon, Mitra Safavi-Naeini, Richard B Banati
This review discusses the use of stable ((13)C, (2)D) or radioactive isotopes ((14)C, (11)C, (18)F, (131)I, (64)Cu, (68)Ga) incorporated into the molecular structure of new drug entities for the purpose of pharmacokinetic or -dynamic studies. Metabolite in safety testing requires the administration of pharmacologically active doses. In such studies, radiotracers find application mainly in preclinical animal investigations, whereby LC-MS/MS is used to identify metabolite structure and drug-related effects. In contrast, first-in-human metabolite studies have to be carried out at nonpharmacological doses not exceeding 100 μg (microdose), which is generally too low for metabolite detection by LC-MS/MS...
November 24, 2017: Bioanalysis
John F Kellie, Jonathan R Kehler, Molly Z Karlinsey, Scott G Summerfield
AIM: Typically, quantitation of biotherapeutics from biological matrices by LC-MS is based on a surrogate peptide approach to determine molecule concentration. Recent efforts have focused on quantitation of the intact protein molecules or larger mass subunits of monoclonal antibodies. To date, there has been limited guidance for large or intact protein mass quantitation for quantitative bioanalysis. METHODOLOGY: Intact- and subunit-level analyses of biotherapeutics from biological matrices are performed at 12-25 kDa mass range with quantitation data presented...
November 24, 2017: Bioanalysis
Jan Welink, Eric Yang, Nicola Hughes, Brian Rago, Eric Woolf, Jens Sydor, Laura Coppola, Brad Ackermann, Wenkui Li, Stephen C Alley, Mark Arnold, Isabella Berger, Chad Briscoe, Michael Buonarati, Mark Bustard, Mark Cancilla, Seongeun Julia Cho, Jeff Duggan, Daniela Fraier, Fabio Garofolo, Rachel Green, Sam Haidar, Lucinda Hittle, Akiko Ishii-Watabe, Rafiq Islam, Rand Jenkins, Barry Jones, John Kadavil, Sean Kassim, Olga Kavetska, Olivier Le Blaye, Anita Lee, Hanlan Liu, John Mehl, Gustavo Mendes Lima Santos, Adrien Musuku, Ragu Ramanathan, Yoshiro Saito, Natasha Savoie, Scott Summerfield, Sekhar Surapaneni, Matthew Szapacs, Nilufer Tampal, Tom Verhaeghe, Stephen Vinter, Emma Whale
The 2017 11th Workshop on Recent Issues in Bioanalysis (11th WRIB) took place in Los Angeles/Universal City, California from 3 April 2017 to 7 April 2017 with participation of close to 750 professionals from pharmaceutical/biopharmaceutical companies, biotechnology companies, contract research organizations and regulatory agencies worldwide. WRIB was once again a 5-day, weeklong event - A Full Immersion Week of Bioanalysis, Biomarkers and Immunogenicity. As usual, it was specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest including both small and large molecule analysis involving LCMS, hybrid LBA/LCMS and ligand-binding assay (LBA) approaches...
November 17, 2017: Bioanalysis
Nikolay Youhnovski, Laurence Mayrand-Provencher, Eugénie-Raphaëlle Bérubé, Jeff Plomley, Hélène Montpetit, Milton Furtado, Anahita Keyhani
AIM: Volumetric absorptive microsampling (VAMS) is a recent technology available for sampling and analyzing low blood volume. The present work describes the utilization of VAMS for the quantitation of naproxen and ritonavir in human blood using a novel bead-based impact-assisted extraction (IAE) procedure. RESULTS: Sampling volume accuracy of the VAMS device was independent of the blood hematocrit (HCT) level, however analyte recovery decreased with increasing HCT when extracted using ultrasonication...
November 17, 2017: Bioanalysis
George R Gunn, Christopher Evans, Eric Yang
No abstract text is available yet for this article.
November 9, 2017: Bioanalysis
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"