Assessment of arsenic oxidation potential of Microvirga indica S-MI1b sp. nov. in heavy metal polluted environment.

Arsenic oxidizing α-proteobacterial strain Microvirga indica S-MI1b sp. nov. was isolated from metal industry soil and has the ability to oxidize 15 mM of arsenite [As(III)] completely in 39 h. The strain S-MI1b resists to different heavy metals and it oxidizes arsenite in presence of Li, Pb, Hg, Sb(III), Cd, Cr(VI), Ni, and exhibited growth inhibitory effect in presence of Hg, Cu, and Cd at higher concentration. The morphology of Microvirga indica S-MI1b changed in presence of heavy metals however there was no accumulation of As(III) in the cells. The study showed that Microvirga indica S-MI1b can oxidize arsenite at broad pH ranges from 4.0 to 9.0 with optimum at pH 7.0. The kinetic studies of arsenite oxidation by strain S-MI1b signified that it has greater affinity towards As(III). The arsenite oxidase activity of cells grown in presence of Li and Cr(VI) supported the cell culture studies. This is first report on biotransformation of arsenite by Microvirga genus and also arsenite oxidation in presence of heavy metals.