Robiginitalea sediminis sp. nov., isolated from a sea cucumber culture pond.

A Gram-stain negative, aerobic, non-motile, orange and rod/coccoid-shaped bacterium, designated O458T , was isolated from sediment collected from a sea cucumber culture pond located in Rongcheng, Shandong province, China (122°14'E, 36°54'N). Comparative analysis of 16S rRNA gene sequences revealed that strain O458T is a member of the genus Robiginitalea and is closely related to Robiginitalea biformata (95.7% sequence similarity) and Robiginitalea myxolifaciens (94.9%). Its DNA G+C content was 55.2 mol%. The strain grew at temperatures between 15 and 45 °C (optimum 33 °C), from pH 6.5 to 8.5 (optimum pH 7.0-7.5) and between 1.0 and 6.0% (w/v) NaCl (optimum 2.0%). Strain O458T was found to contain menaquinon-6 (MK-6) as the only respiratory quinone, iso-C15:0 , iso-C17:0 3-OH and Summed feature 1 (iso-C15:1 H and/or iso-C13:0 3-OH) as the major fatty acids. The major polar lipids were phosphatidylethanolamine (PE) and four unidentified lipids (L1, L2, L3 and L4). On the basis of the results of the phylogenetic and phenotypic analyses it is suggested that strain O458T represents a novel species of the genus Robiginitalea, for which the name Robiginitalea sediminis sp. nov. is proposed. The type strain is O458T (=KCTC 52898T =MCCC 1H00190T ).