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Antonie Van Leeuwenhoek

Hyosun Lee, Dong-Uk Kim, Sooyeon Park, Jung-Hoon Yoon, Jong-Ok Ka
A Gram-stain negative, rod-shaped, aerobic bacterial strain, designated TA-C7e(T), was isolated from an agricultural soil obtained from Taean region, Korea. Phylogenetically, the strain was grouped with the genus Massilia and shared 95.33-97.97% 16S rRNA sequence similarity with the type strains of other species of the genus Massilia. The DNA-DNA hybridisation between TA-C7e(T) and Massilia haematophila KACC 13771(T), Massilia suwonesis KACC 12635(T), and Massilia yuzhufengensis KACC 16569(T) revealed a low level of relatedness (<40%)...
April 22, 2017: Antonie Van Leeuwenhoek
Katarzyna Zamlynska, Iwona Komaniecka, Kamil Zebracki, Andrzej Mazur, Anna Sroka-Bartnicka, Adam Choma
The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02(T), a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-D-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, substituted by galacturonic acid (GalpA) at position C-1 and partly decorated by a phosphate residue at C-4' of the non-reducing GlcpN3N. Both diaminosugars were symmetrically substituted by 3-hydroxy fatty acids (14:0(3-OH) and 16:0(3-OH))...
April 13, 2017: Antonie Van Leeuwenhoek
Casper Fredsgaard, Donald B Moore, Fei Chen, Benton C Clark, Mark A Schneegurt
Sucretolerant microbes grow in the presence of sugar concentrations high enough to substantially lower water activities. Natural habitats high in sugars are mainly limited to dried fruit, floral nectar, honey, sugarcane, and associated soils. Organisms that tolerate extremes of solute concentration, high enough to lower water activities, might not be expected in common oligoosmotic soils. We report on the isolation of sucretolerant bacteria from common soils using media supplemented with 50% sucrose (a w 0...
April 13, 2017: Antonie Van Leeuwenhoek
Jaewoo Yoon, So Young Kim, Songhee Park, Hyukjae Choi
A Gram-negative, strictly aerobic, chemoheterotrophic, beige-pigmented, ovoid bacterium, designated YP194(T), was isolated from marine sediment in the Republic of Korea. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain belongs to the family Rhodobacteraceae of the class Alphaproteobacteria, with high sequence similarity (98.4%) to Marinovum algicola FF3(T). The DNA-DNA relatedness values between strains YP194(T) and M. algicola FF3(T) were 34.1 ± 2.7%. The DNA G+C content of strain YP194(T) was 63...
April 11, 2017: Antonie Van Leeuwenhoek
Stephanus N Venter, Marike Palmer, Chrizelle W Beukes, Wai-Yin Chan, Giyoon Shin, Elritha van Zyl, Tarren Seale, Teresa A Coutinho, Emma T Steenkamp
Bacterial species are commonly defined by applying a set of predetermined criteria, including DNA-DNA hybridization values, 16S rRNA gene sequence similarity, phenotypic data as well as genome-based criteria such as average nucleotide identity or digital DNA-DNA hybridization. These criteria mostly allow for the delimitation of taxa that resemble typical bacterial species. Their application is often complicated when the objective is to delineate new species that are characterized by significant population-level diversity or recent speciation...
April 9, 2017: Antonie Van Leeuwenhoek
Rita Abou Abdallah, Mamadou Beye, Awa Diop, Sofiane Bakour, Didier Raoult, Pierre-Edouard Fournier
Over the past decade, new culture methods coupled to genome and metagenome sequencing have enabled the number of isolated bacterial species with standing in nomenclature to rise to more than 15,000 whereas it was only 1791 in 1980. 'Culturomics', a new approach based on the diversification of culture conditions, has enabled the isolation of more than 1000 distinct human-associated bacterial species since 2012, including 247 new species. This strategy was demonstrated to be complementary to metagenome sequencing for the exhaustive study of the human microbiota and its roles in health and diseases...
April 7, 2017: Antonie Van Leeuwenhoek
Lucas R Ribeiro, Ana R O Santos, Marizeth Groenewald, Maudy Th H Smith, Carla A Lara, Aristóteles Góes-Neto, Noémie Jacques, Cécile Grondin, Serge Casaregola, Marc-André Lachance, Carlos A Rosa
During studies of the yeast diversity associated with rotting wood in Brazil and fruits, plants and insects in French Guiana, three strains of a new species were isolated. Analysis of the sequences of the internal transcribed spacer (ITS)-5.8S and D1/D2 domains of the large subunit of the rRNA gene showed that this species belongs to the genus Hyphopichia and its closest relative is Candida homilentoma. These species differ by 44 nucleotide substitutions in D1/D2 sequences. A new species Hyphopichia buzzinii f...
April 6, 2017: Antonie Van Leeuwenhoek
Agnieszka Kalwasińska, Tamás Felföldi, Attila Szabó, Edyta Deja-Sikora, Przemysław Kosobucki, Maciej Walczak
Soda lime is a by-product of the Solvay soda process for the production of sodium carbonate from limestone and sodium chloride. Due to a high salt concentration and alkaline pH, the lime is considered as a potential habitat of haloalkaliphilic and haloalkalitolerant microbial communities. This artificial and unique environment is nutrient-poor and devoid of vegetation, due in part to semi-arid, saline and alkaline conditions. Samples taken from the surface layer of the lime and from the depth of 2 m (both having pH ~11 and ECe up to 423 dS m(-1)) were investigated using culture-based (culturing on alkaline medium) and culture-independent microbiological approaches (microscopic analyses and pyrosequencing)...
April 5, 2017: Antonie Van Leeuwenhoek
Flaviana Di Lorenzo
Suberites domuncula is a marine demosponge harbouring a large bacterioflora, including commensal, opportunistic and pathogenic bacteria, among which, species of the Gram-negative genus Pseudoalteromonas were identified. The sponge-bacteria interaction mechanisms are still not fully understood. As the main component of the Gram-negative bacterial outer membrane, the lipopolysaccharide (LPS) may play a role in such a crucial relationship. Moreover, the LPS is known to be the most versatile bioactive macromolecule of Gram-negative bacteria and its lipid A structure is responsible for the immunological activity of the whole LPS on eukaryotic host cells...
March 31, 2017: Antonie Van Leeuwenhoek
F Nievas, L Vilchez, W Giordano, P Bogino
A wide variety of plant-associated soil bacteria (rhizobacteria) communicate with each other by quorum sensing (QS). Plants are able to detect and produce mimics and inhibitor molecules of the QS bacterial communicative process. Arachis hypogaea L. (peanut) establishes a nitrogen-fixing symbiosis with rhizobia belonging to the genus Bradyrhizobium. These bacteria use a QS mechanism dependent on the synthesis of N-acyl homoserine lactones (AHLs). Given the relevance that plant-rhizobacteria interactions have at the ecological level, this work addresses the involvement of peanut in taking part in the QS mechanism...
March 29, 2017: Antonie Van Leeuwenhoek
Venkadasamy Govindasamy, Susheel Kumar Raina, Priya George, Mahesh Kumar, Jagadish Rane, Paramjit Singh Minhas, Kanuparthy Pandu Ranga Vittal
A diverse group of bacteria colonize the exo- and endo-rhizospheres of sorghum and play a critical role in its tolerance to drought and other abiotic stresses. Two hundred and eighty endophytic bacteria were isolated from the surface-sterilized roots of four sorghum cultivars that were grown on three soil types at three different phenological stages of growth. The isolates were subjected to in vitro screening for their plant growth promoting traits. Out of 280 isolates, 70 could produce Indole 3-Acetic Acid (IAA), 28 showed N-fixation, 28 could solubilize phosphate, 24 had ACC deaminase activity and 13 isolates were able to produce siderophores...
March 28, 2017: Antonie Van Leeuwenhoek
Elham Mohammadian, Mahdi Arzanlou, Asadollah Babai-Ahari
The present study was aimed at characterising species diversity of fungi inhabiting petroleum-contaminated soils of oil fields in a southern region of Iran. Two different techniques were used for fungal isolation including enrichment on atmospheres of phenolic hydrocarbons and crude oil as substrate. Phylogenetic analysis of the internal transcribed spacer of ribosomal DNA was used for taxonomic identification with additional information from the β-tubulin gene for selected taxa. Overall, 183 strains from 14 genera and five orders were obtained: Pleosporales (Alternaria, Curvularia, Stemphylium, Ulocladium), Chaetothyriales (Exophiala), Eurotiales (Aspergillus), Hypocreales (Acremonium, Emericellopsis, Sarocladium, Stachybotrys, Fusarium, Trichoderma, Beauveria), and Capnodiales (Cladosporium)...
March 28, 2017: Antonie Van Leeuwenhoek
Pedro Geadas Farias, Fernando Gama, Diogo Reis, Susana Alarico, Nuno Empadinhas, José Carlos Martins, Ana Figueiredo de Almeida, Paula Vasconcelos Morais
Hospital environmental conditions, human occupancy, and the characteristics of the equipment influence the survival of microbial communities and raise a concern with regard to nosocomial infections. The objective of the present work was to use the monitoring of Pseudomonas aeruginosa, Klebsiella spp. and non-tuberculous mycobacteria as a strategy to improve knowledge on microbial colonization of non-critical equipment and surfaces, in a tertiary hospital from Central Portugal. A 3-month microbiological survey was performed in a district teaching hospital...
March 23, 2017: Antonie Van Leeuwenhoek
Yili Huang, Hao Feng, Hang Lu, Yanhua Zeng
It is believed that sphingomonads are ubiquitously distributed in environments. However detailed information about their community structure and their co-relationship with environmental parameters remain unclear. In this study, novel sphingomonads-specific primers based on the 16S rRNA gene were designed to investigate the distribution of sphingomonads in 10 different niches. Both in silico and in-practice tests on pure cultures and environmental samples showed that Sph384f/Sph701r was an efficient primer set...
March 23, 2017: Antonie Van Leeuwenhoek
Immacolata Speciale, Irina Agarkova, Garry A Duncan, James L Van Etten, Cristina De Castro
Results from recent studies are breaking the paradigm that all viruses depend on their host machinery to glycosylate their proteins. Chloroviruses encode several genes involved in glycan biosynthesis and some of their capsid proteins are decorated with N-linked oligosaccharides with unique features. Here we describe the elucidation of the N-glycan structure of an unusual chlorovirus, NE-JV-1, that belongs to the Pbi group. The host for NE-JV-1 is the zoochlorella Micractinium conductrix. Spectroscopic analyses established that this N-glycan consists of a core region that is conserved in all of the chloroviruses...
March 22, 2017: Antonie Van Leeuwenhoek
Milagro Fernández-Delgado, Paula Suárez, Sandra Giner, Virginia Sanz, Jessy Peña, Damarys Sánchez, M Alexandra García-Amado
The Vibrionaceae are Gram-negative bacteria present in marine and estuarine environments worldwide, including several species known as important pathogens to humans and aquatic organisms. The aim of this research was to investigate the occurrence and virulence properties of Vibrio and Salinivibrio isolated from lagoons at Cuare Wildlife Refuge and Margarita Island in the southern Caribbean Sea. Water, plankton and oyster samples were collected during October 2011 and March 2012 and examined by specific PCR and culture methods...
March 21, 2017: Antonie Van Leeuwenhoek
Hamidah Idris, Imen Nouioui, Juan A Asenjo, Alan T Bull, Michael Goodfellow
The taxonomic position of a filamentous actinobacterial strain, isolate H45(T), recovered from a high altitude Atacama Desert gravel soil, was established using a polyphasic approach. The strain, which is known to produce novel dienes, has chemotaxonomic and morphological properties typical of the genus Lentzea and formed a distinct branch in the Lentzea 16S rRNA gene tree that is closely related to the type strain of Lentzea kentuckyensis. The two strains were distinguished using a combination of phenotypic properties and by a DNA:DNA relatedness value of 37...
March 21, 2017: Antonie Van Leeuwenhoek
Munusamy Madhaiyan, Selvaraj Poonguzhali, Venkatakrishnan Sivaraj Saravanan, Kumaran Selvapravin, Veeramuthu Duraipandiyan, Naif Abdullah Al-Dhabi
A novel Gram-stain negative, aerobic, motile, rod-shaped bacterium was isolated from Sesame (Sesamum indicum L.) rhizosphere soil. Based on the 16S rRNA gene similarity value (99.4-98.6%) obtained with phylogenetically closely related strains and through analyses of their house keeping genes (atpD, infB and rpoB), the strain SI-P133(T) was delineated among the species of the genus Pseudomonas and was subjected to polyphasic taxonomic analysis. It was a chemoorganotroph which grew at wide range of temperature (4-45 °C), pH (5...
March 20, 2017: Antonie Van Leeuwenhoek
Hatnagar Sarang, Pijakala Rajani, Madhugiri Mallaiah Vasanthakumari, Patel Mohana Kumara, Ramamoorthy Siva, Gudasalamani Ravikanth, R Uma Shaanker
Lawsone (2-hydroxy-1, 4-napthoquinone), also known as hennotannic acid, is an orange red dye used as a popular skin and hair colorant. The dye is produced in the leaves of Lawsonia inermis L, often referred to as the "henna" tree. In this study, we report the production of lawsone by an endophytic fungus, Gibberella moniliformis isolated from the leaf tissues of Lawsonia inermis. The fungus produced the orange-red dye in potato dextrose agar and broth, independent of the host tissue. Presence of lawsone was confirmed spectrometrically using HPLC and ESI-MS/MS analysis...
March 17, 2017: Antonie Van Leeuwenhoek
Tarek A A Moussa, Hassan S Al-Zahrani, Naif M S Kadasa, Sarah A Ahmed, G Sybren de Hoog, Abdullah M S Al-Hatmi
Two new species in the Fusarium fujikuroi species complex (FFSC) are introduced. One of these, represented by strain CBS 454.97 was isolated from plant debris (Striga hermonthica) in the Sudan, while the second, represented by strains CBS 119850 and CBS 483.94, which originated from soil in Australia. Molecular analyses were performed including TEF1 spanning 576 bp region, 860 bp region of rPB2, and 500 bp BT2 region. Phylogenetic trees based on these regions showed that the two species are clearly distinct from all known taxa in the F...
March 16, 2017: Antonie Van Leeuwenhoek
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