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Antonie Van Leeuwenhoek

Lei Dong, Ming-Xian Han, Dong Wang, Feng Liu, Mipeshwaree Devi Asem, Jian-Yu Jiao, Min Xiao, Nimaichand Salam, Wen-Jun Li
A motile, rod-shaped and yellow coloured proteobacterium, designated strain SYSU D60017T , was isolated from a desert soil sample. The bacterium was found to be an obligately aerobic, mesophilic and neutrophilic chemo-heterotroph. Cells were observed to be Gram-stain negative, catalase positive and oxidase positive. The major cellular fatty acids were identified as C19:0 ω8c cyclo and Summed Feature 8 (C18:1 ω7c and/or C18:1 ω6c). The main respiratory quinone identified was ubiquinone-10. The DNA G + C content was determined to be 63...
January 14, 2019: Antonie Van Leeuwenhoek
Xiao-Rui Yan, Li Tuo
A Gram-stain negative, aerobic, coccus-shaped bacterium, designated strain M2BS9Y-3-1T , was isolated from a surface-sterilized leaf of Rhizophora stylosa. It grew in the pH range 5.0-9.0 (optimum, 7.0) and at temperatures between 20 and 37 °C (optimum, 30 °C). Growth was possible at concentrations of 1-10% (w/v) NaCl (optimum, 2%). The phylogenetic trees based on 16S rRNA gene sequences indicated that strain M2BS9Y-3-1T clustered together with Notoacmeibacter marinus XMTR2A4T (98.44% similarity) and separated from other species of the family Notoacmeibacteraceae...
January 10, 2019: Antonie Van Leeuwenhoek
Ramon Rosselló-Móra, Iain C Sutcliffe
No abstract text is available yet for this article.
January 9, 2019: Antonie Van Leeuwenhoek
Bao-Zhu Fang, Ming-Xian Han, Jian-Yu Jiao, Xiao-Tong Zhang, Yuan-Guo Xie, Wael N Hozzein, Dalal Hussien M Alkhalifah, Min Xiao, Wen-Jun Li
A novel actinobacterial strain, designated YIM ART13T , was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, South western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were found to aerobic and Gram-stain positive. On the basis of 16S rRNA gene sequence analysis, strain YIM ART13T was found to be closely related to Nocardioides pakistanensis NCCP 1340T (96.1% sequence similarity) and is therefore considered to represent a member of the genus Nocardioides...
January 2, 2019: Antonie Van Leeuwenhoek
Hilal Ay, Imen Nouioui, Lorena Carro, Hans-Peter Klenk, Demet Cetin, José M Igual, Nevzat Sahin, Kamil Isik
A novel actinobacterial strain, designated GTF31T , was isolated from a coastal soil sample of Gölcük Lake, a crater lake in southwest Anatolia, Turkey. The taxonomic position of the strain was established using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences and showed that the strain is closely related to Jiangella gansuensis DSM 44835T (99.4%), Jiangella alba DSM 45237T (99.3%) and Jiangella muralis DSM 45357T (99.2%). Optimal growth was observed at 28 °C and pH 7-8. Whole cell hydrolysates were found to contain LL-DAP, glucose, mannose, rhamnose and ribose...
January 2, 2019: Antonie Van Leeuwenhoek
Min-Ling Zheng, Jian-Yu Jiao, Lei Dong, Ming-Xian Han, Liang-Hui Li, Min Xiao, Cha Chen, Ping-Hua Qu, Wen-Jun Li
A Gram-negative, aerobic, non-motile and non-spore forming bacterium, designated strain SYSU WZ-2T , was isolated from an estuarine seawater sample. Growth of strain SYSU WZ-2T was observed at temperature range of 10-40° C (optimum, 32 °C), pH range of 6-10 (optimum, pH 7-8) and in the presence of up to 5.0% NaCl (w/v). The DNA G+C content of the novel strain was determined to be 30.1% (genome). The major polar lipids were found to be diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid, two unidentified aminophospholipids and two unidentified phospholipids...
January 2, 2019: Antonie Van Leeuwenhoek
Renju Liu, Zhaobin Huang, Chunming Dong, Zongze Shao
A taxonomic study was carried out on strain SA5d-4T , which was isolated from a marine limpet (Patelloida saccharina lanx [Reeve, 1855]) collected from intertidal rocks in Xiamen, China. Strain SA5d-4T was aerobic, Gram-positive, lacked flagellum, non-motile, filamentous, formed a slightly-yellowish colony, and non-sporulating. The strain grew optimally at 28 °C, at pH values 7.0-8.0, and in the presence of 1-2% (w/v) sodium chloride. The major cellular fatty acids identified were iso-C15:0 , iso-C17:0 ω10c, and iso-C17:0 ...
January 1, 2019: Antonie Van Leeuwenhoek
Mariana Caroline Tocantins Alvim, Camilo Elber Vital, Edvaldo Barros, Nívea Moreira Vieira, Fernando Augusto da Silveira, Thércia Rocha Balbino, Raphael Hermano Santos Diniz, Amanda Fernandes Brito, Denise Mara Soares Bazzolli, Humberto Josué de Oliveira Ramos, Wendel Batista da Silveira
Kluyveromyces marxianus CCT 7735 offers advantages to ethanol production over Saccharomyces cerevisiae, including thermotolerance and the ability to convert lactose to ethanol. However, its growth is impaired at high ethanol concentrations. Herein we report on the protein and intracellular metabolite profiles of K. marxianus at 1 and 4 h under ethanol exposure. The concentration of some amino acids, trehalose and ergosterol were also measured. We observed that proteins and metabolites from carbon pathways and translation were less abundant, mainly at 4 h of ethanol stress...
January 1, 2019: Antonie Van Leeuwenhoek
Shuai-Bo Han, Xin-Jun Hou, Yang-Huan Yu, Zhao Ju, Ran Zhang, Rui-Jun Wang, Yang-Hui Ye, Yan-Hu Ren, Wei-Yan Zhang, Min Wu
A Gram-stain negative, non-motile, strictly aerobic and rod-shaped bacterium, designated as 15181T , was isolated from a salt lake in Xinjiang Province, China. Strain 15181T was able to grow at 10-40 °C (optimum 37 °C), pH 6.0-8.5 (optimum 7.0) and with 1-14% NaCl (optimum 4%, w/v). According to phylogenetic analysis based on 16S rRNA gene sequences, strain 15181T was assigned to the genus Wenzhouxiangella with high 16S rRNA gene sequence similarity of 97.4% to Wenzhouxiangella sediminis XDB06T , followed by Wenzhouxiangella marina KCTC 42284T (95...
December 19, 2018: Antonie Van Leeuwenhoek
Jae-Chan Lee, Jun-Soo Song, Kyung-Sook Whang
A Gram-stain negative, aromatic compound degrading bacterium, designated strain ASA28T , was isolated from the rhizosphere soil of a pine tree at Anmyon island, Taean in Korea. Strain ASA28T was found to be strictly aerobic, non-motile, short rods which can grow at 15-28 °C (optimum, 25-28 °C), at pH 5.0-11.0 (optimum, pH 7.0) and at salinities of 0-1.0% (w/v) NaCl (optimum, 0% NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain ASA28T belongs to the genus Sphingobium, showing high sequence similarity to Sphingobium scionense WP01T (97...
December 18, 2018: Antonie Van Leeuwenhoek
Bruno Senghor, Hubert Bassène, Saber Khelaifia, Catherine Robert, Pierre-Edouard Fournier, Raymond Ruimy, Cheikh Sokhna, Didier Raoult, Jean-Christophe Lagier
Oceanobacillus timonensis Marseille-P3532T (CSUR P3532, CCUG 70981) and Oceanobacillus senegalensis Marseille-P3587T (CSUR P3587, CCUG 70613), are the type strains of O. timonensis sp. nov. and O. senegalensis sp. nov., respectively. They are moderately halophilic, aerobic, motile and Gram-stain positive bacteria. The strains P3532T and P3587T were isolated from stools with 3.8% and 2.1% sodium chloride (NaCl) of healthy 10 year old female and male 7-year-old children, respectively and living respectively at Dielmo and N'diop two villages in Senegal (West Africa)...
December 15, 2018: Antonie Van Leeuwenhoek
Pranitha S Pandit, Monali C Rahalkar
Candidatus Methylocucumis (Mcu.) oryzae, strain Sn10-6, a Gram-negative, pale pink pigmented, motile, large Type I methanotroph (3-6 µm × 1.5-2 µm) was recently isolated from an Indian rice field. Due to the difficulties in preservation of the strain it could not be deposited and preserved in two recognised culture collections with public access located in two different countries. Hence, Sn10-6 was described as a member of a Candidatus genus and species, 'Ca. Mcu. oryzae'. Subsequent to the publication, the strain has been preserved in the Japan Collection of Microorganisms, Japan (JCM 32869T ) in addition to its earlier preservation in the Korean collection for type cultures, KCTC, Korea and in National Centre for Microbial Resources, India...
December 15, 2018: Antonie Van Leeuwenhoek
Viacheslav Spirin, Vera Malysheva, Danny Haelewaters, Karl-Henrik Larsson
Stypella vermiformis is a heterobasidiomycete producing minute gelatinous basidiocarps on rotten wood of conifers in the Northern Hemisphere. In the current literature, Stypella papillata, the genus type of Stypella (described from Brazil), is treated as a taxonomic synonym of S. vermiformis. In the present paper, we revise the type material of S. papillata and a number of specimens addressed to S. vermiformis. As a result, the presumed synonymy of S. papillata and S. vermiformis is rejected and the genus Stypella is restricted to the single species S...
December 8, 2018: Antonie Van Leeuwenhoek
Jia Song, Shiwen Qiu, Junwei Zhao, Chuanyu Han, Ying Wang, Xiujun Sun, Shanwen Jiang, Xiangjing Wang, Wensheng Xiang
An aerobic, non-motile, Gram-stain positive actinomycete, designated strain NEAU-YY211T , was isolated from the rhizosphere soil of wheat (Triticum aestivum L.) collected from Zhumadian, Henan Province, mid-eastern China, and characterised taxonomically using a polyphasic approach. Comparative analysis of the 16S rRNA gene sequence indicated that strain NEAU-YY211T belongs to the genus Pseudonocardia, showing high similarities with respect to Pseudonocardia ammonioxydans H9T (99.1%) and Pseudonocardia antitumoralis SCSIO 01299T (99...
December 7, 2018: Antonie Van Leeuwenhoek
Valeriya V Kurilenko, Lyudmila A Romanenko, Marina P Isaeva, Vassilii I Svetashev, Valery V Mikhailov
An aerobic, Gram-negative, yellow-pigmented non-motile rod-shaped bacterium Kr9-9T was isolated from a brown alga specimen collected near the Kuril Islands. Based on the 16S rRNA gene sequence analysis strain Kr9-9T was assigned to the genus Winogradskyella, and its close phylogenetic neighbors were found to be Winogradskyella damuponensis KCTC 23552T , Winogradskyella sediminis LMG 28075T , and Winogradskyella rapida CCUG 59098T showing high similarities of 98.1%, 97.5%, and 97.1%, respectively. It contained MK-6 as the predominant menaquinone and iso-C15:0 , anteiso-C15:0 , iso-C16:0 3-OH followed by iso-C15:1 as the major fatty acids...
December 5, 2018: Antonie Van Leeuwenhoek
S Y But, S V Egorova, V N Khmelenina, Y A Trotsenko
The indicator enzyme of the serine pathway of assimilation of reduced C1 compounds, serine-glyoxylate aminotransferase (Sga), has been purified from three methane-oxidizing bacteria, Methylomicrobium alcaliphilum 20Z, Methylosinus trichosporium OB3b and Methylococcus capsulatus Bath. The native enzymes were shown to be dimeric (80 kDa, strain 20Z), tetrameric (~ 170 kDa, strain OB3b) or trimeric (~ 120 kDa, strain Bath). Sga from the three methanotrophs catalyse the pyridoxal phosphate-dependent transfer of an amino group from serine to glyoxylate and pyruvate; the enzymes from strains 20Z and Bath also transfer an amino group from serine to α-ketoglutarate and from alanine to glyoxylate...
December 3, 2018: Antonie Van Leeuwenhoek
Yingshun Cui, Seong-Jun Chun, A-Ra Cho, Shu Kuan Wong, Hyung-Gwan Lee, Hee-Mock Oh, Chi-Yong Ahn
A novel Gram-stain negative, rod-shaped and motile bacterial strain, designated strain Seoho-38T , was isolated from a eutrophic lake in South Korea. Polyphasic taxonomic studies were performed to investigate the taxonomic position of the new isolate. The phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain Seoho-38T formed a distinct cluster with Nevskia ramosa Soe1T , Nevskia persephonica G6M-30T , Nevskia soli GR15-1T , Nevskia terrae KIS13-15T and Nevskia aquatilis F2-63T with bootstrap resampling value of 100%...
November 30, 2018: Antonie Van Leeuwenhoek
Jiwon Choi, Seho Cha, Geeta Chhetri, Dahye Yang, Taegun Seo
A short rod-shaped, yellow-orange pigmented, strictly aerobic bacterium, designated as strain H2T , was isolated from the wetland soil of Halla Mountain, Jeju-island, South Korea. Growth was observed at temperatures of 10-30 °C (optimum at 25-30 °C), pH of 6-8 (optimum at pH 7), and salt concentrations of 0-1% (w/v) NaCl (optimum at 0%). The strain H2T was found to be a catalase and oxidase-positive, non-motile, Gram-negative bacterium. On the basis of 16S rRNA gene sequence similarity and phylogenetic analysis, strain H2T was found to be related to the members of the Chitinophagaceae family, being closely related to Taibaiella chishuiensis AY17T (94...
November 24, 2018: Antonie Van Leeuwenhoek
Chonglu Zhong, Yong Zhang, Yongcheng Wei, Jingxiang Meng, Yu Chen, David Bush, Didier Bogusz, Claudine Franche
Casuarina trees are planted along the coast from Hainan province in South China to the Zhoushan Islands of Zhejiang province in Southeastern China. Three key species, Casuarina equisetifolia, Casuarina cunninghamiana and Casuarina glauca, are used as windbreaks, in agroforestry systems, and for the production of timber and fuel wood. Frankia have been studied in China since 1984. Today, Frankia research fields are very wide, and cover morphology, physiology and genetic diversity, and the application of inocula for specific purposes on poor quality sites...
November 24, 2018: Antonie Van Leeuwenhoek
Nisanart Charoenlap, Luksika Jiramonai, Jurairat Chittrakanwong, Naruemon Tunsakul, Skorn Mongkolsuk, Paiboon Vattanaviboon
Inactivation of ahpC, encoding alkyl hydroperoxide reductase, rendered Stenotrophomonas maltophilia more resistant to H2 O2 ; the phenotype was directly correlated with enhanced total catalase activity, resulting from an increased level of KatA catalase. Plasmid-borne expression of ahpC from pAhpCsm could complement all of the mutant phenotypes. Mutagenesis of the proposed AhpC peroxidactic and resolving cysteine residues to alanine (C47A and C166A) on the pAhpCsm plasmid diminished its ability to complement the ahpC mutant phenotypes, suggesting that the mutagenized ahpC was non-functional...
November 22, 2018: Antonie Van Leeuwenhoek
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