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Antonie Van Leeuwenhoek

Yi-Rui Yin, Wen-Dong Xian, Ming-Xian Han, En-Min Zhou, Lan Liu, Dalal Hussien M Alkhalifah, Wael N Hozzein, Min Xiao, Wen-Jun Li
A gene encoding a β-xylosidase (designated as Thxyl43A) was cloned from strain Thermobifida halotolerans YIM 90462T . The open reading frame of this gene encodes 550 amino acid residues. The gene was over-expressed in Escherichia coli and the recombinant protein was purified. The monomeric Thxyl43A protein presented a molecular mass of 61.5 kDa. When p-nitrophenyl-β-d-xylopyranoside was used as the substrate, recombinant Thxyl43A exhibited optimal activity at 55 °C and pH 4.0 to 7.0, being thermostable by maintaining 47% of its activity after 30 h incubation at 55 °C...
September 17, 2018: Antonie Van Leeuwenhoek
Lan-Yu Li, Zi-Wen Yang, Mipeshwaree Devi Asem, Bao-Zhu Fang, Nimaichand Salam, Dalal Hussien M Alkhalifah, Wael N Hozzein, Guo-Xing Nie, Wen-Jun Li
A Streptomyces isolate, designated strain SYSU D8023T , was isolated from a desert sand sample collected from Gurbantunggut desert, China. The characterisation of the isolate was achieved using a polyphasic taxonomic approach. The isolate was found to be Gram-positive and aerobic. The strain was found to be able to grow at 14-50 °C, pH 6.0-9.0 and in the presence of up to 7% (w/v) NaCl. Strain SYSU D8023T contains LL-diaminopimelic acid as a cell wall diamino acid. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified glycolipid and an unidentified phospholipid...
September 17, 2018: Antonie Van Leeuwenhoek
Onuma Kaewkla, Christopher Milton Mathew Franco
An endophytic actinobacterium, strain CAP 335T , was isolated from a root sample of a native pine tree growing on the Bedford Park campus of Flinders University, Adelaide, South Australia. The result of a polyphasic study showed that this strain was identified as a new member of the genus Actinomycetospora. This strain was observed to be a Gram stain-positive, aerobic actinobacterium with well-developed substrate mycelia and to form short chains of spores. Actinomycetospora chibensis TT04-21T and Actinomycetospora straminea IY07-55T were found to be close phylogenetic neighbours, each sharing 99...
September 17, 2018: Antonie Van Leeuwenhoek
Bruna Cristina Custodio, Andre Rodrigues
Parasite-host associations are widespread in nature and the fungus-growing ants are considered model organisms to study such interactions. These insects cultivate basidiomycetous fungi for food, which are threatened by mycotrophic fungi in the genus Escovopsis. Although recently described from colonies of the lower attine ant Mycetophylax morschi, the biology and pathogenicity of Escovopsis kreiselii are unknown. Herein, we evaluated the interaction of E. kreiselii with fungi cultivated by M. morschi (native hosts) and with a fungus cultivated by another attine ant species (non-native host)...
September 11, 2018: Antonie Van Leeuwenhoek
Faten Ghodhbane-Gtari, Imen Nouioui, Karima Hezbri, Emily Lundstedt, Timothy D'Angelo, Zakkary McNutt, Laurent Laplaze, Hassen Gherbi, Virginie Vaissayre, Sergio Svistoonoff, Hela Ben Ahmed, Abdelatif Boudabous, Louis S Tisa
Actinorhizal plants form a symbiotic association with the nitrogen-fixing actinobacteria Frankia. These plants have important economic and ecological benefits including land reclamation, soil stabilization, and reforestation. Recently, many non-Frankia actinobacteria have been isolated from actinorhizal root nodules suggesting that they might contribute to nodulation. Two Nocardia strains, BMG51109 and BMG111209, were isolated from Casuarina glauca nodules, and they induced root nodule-like structures in original host plant promoting seedling growth...
September 10, 2018: Antonie Van Leeuwenhoek
Tingting Yang, Andreas Teske, Wallace Ambrose, Verena Salman-Carvalho, Robert Bagnell, Lars Peter Nielsen
We investigated the intracellular dynamics of calcite and sulfur in the large sulfur-oxidizing, calcite-accumulating bacterium Achromatium, with an emphasis on oxygen exposure as a physiological control. For this purpose, morphological changes and possible accretion mechanisms of calcite granules in cells that were freshly collected from natural Achromatium-containing sediment were compared to cells from the same source after prolonged exposure to atmospheric oxygen. Intracellular sulfur is oxidized and removed in response to oxygen exposure...
September 7, 2018: Antonie Van Leeuwenhoek
Adriana Parada Dias da Silveira, Raquel de Paula Freitas Iório, Fernanda Castro Correia Marcos, Ana Olívia Fernandes, Silvana Aparecida Creste Dias de Souza, Eiko Eurya Kuramae, Matheus Aparecido Pereira Cipriano
Few studies have evaluated endophytic bacteria in relation to plant growth promotion, nitrogen uptake and biological control. The aim of this study was to molecularly and physiologically characterize thirteen endophytic bacteria strains, evaluate their biological control properties and their ability to promote plant growth and plant N nutrition. All the strains produced indole acetic acid and promoted increase of plant biomass, N accumulative amount and N-use efficiency index. None of the strains carries the nifH gene...
September 7, 2018: Antonie Van Leeuwenhoek
Céline Pesce, Victoria A Kleiner, Louis S Tisa
Molecular analysis of the filamentous actinobacteria Frankia is laborious because of the slow growth rate and required biomass needed for these techniques. An efficient and simple colony PCR protocol for Frankia was developed that saved time for analysis of any Frankia strains growing on a plate. Previously, it took 5-6 weeks to get the correct size Frankia colonies on plates and then a minimum of 5 weeks of growth in liquid culture for DNA extraction. With this technique, these colonies could be screened after 5-6 weeks of growth by colony PCR...
September 5, 2018: Antonie Van Leeuwenhoek
Rubén Peña, María Angélica Ganga
Brettanomyces bruxellensis negatively impacts on the sensorial quality of wine by producing phenolic compounds associated with unpleasant odors. Thus, the control of this spoilage yeast is a critical factor during the winemaking process. A recent approach used to biocontrol undesired microorganisms is the use of yeast released antimicrobial peptides (AMPs), but this strategy has been poorly applied to wine-related microorganisms. The aim of this study was to evaluate the antifungal capacity of Candida intermedia LAMAP1790 against wine-spoilage strains of B...
September 5, 2018: Antonie Van Leeuwenhoek
Lorena Escudero, Nia Oetiker, Karem Gallardo, Cinthya Tebes-Cayo, Mariela Guajardo, Claudia Nuñez, Carol Davis-Belmar, J J Pueyo, Guillermo Chong Díaz, Cecilia Demergasso
In Table 1 of the original article, the unit mg/L was incorrectly published as ng/L in the aluminum, chloride, sulphate and OM columns.
September 3, 2018: Antonie Van Leeuwenhoek
Ping Hang, Long Zhang, Xi-Yi Zhou, Qiang Hu, Jian-Dong Jiang
A novel Gram-stain negative, facultatively anaerobic, non-spore-forming, motile and rod-shaped bacterium (NS-104T ) was isolated from a propanil-contaminated soil in Nanjing, China. Growth occurred at pH 5.0-9.0 (optimum 6.0), 16-37 °C (optimum 30 °C) and in the presence of 0-2.0% (w/v) NaCl (optimum, without NaCl). Strain NS-104T showed high 16S rRNA gene sequence identity to Rhizobium azooxidifex DSM 100211T (96.7%). The phylogenetic analysis of the 16S rRNA gene as well as the housekeeping genes recA, atpD and glnA demonstrated that strain NS-104T belongs to the genus Rhizobium...
September 3, 2018: Antonie Van Leeuwenhoek
Min-Jiao Liu, Chun-Zhi Jin, Ainijiang Ersiman, Dong-Jin Park, Chang-Jin Kim
A Gram-stain negative strain, designated AN120636T , was isolated from a soil sample collected from Goesan-gun, Chungbuk, South Korea. The strain was strictly aerobic, with golden yellow-pigmented colonies on R2A agar. Cells were non-motile, long or short rods and some were observed to be coccal. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain AN120636T belongs to the genus Flavitalea in the family of Chitinophagacea, with Flavitalea populi (95.4%), Pseudoflavitalea soli (95.0%), Flavitalea gansuensis (94...
September 1, 2018: Antonie Van Leeuwenhoek
Indrani Sarkar, Maher Gtari, Louis S Tisa, Arnab Sen
Protein functional domains are semi-autonomous parts of proteins capable of functioning independently. One protein may contain several domains and one domain may be present in different protein sequences. Thus, protein domains represent the niche specific adaptive nature of an organism. We hypothesized that the presence and absence of protein domains in an organism could be used to make a phylogenetic tree, which may better depict the biotope (niche). Here, we selected 100 actinobacteria and built a phylogenetic tree depending upon the presence and absence of protein domains...
August 31, 2018: Antonie Van Leeuwenhoek
Nattakorn Kuncharoen, Takuji Kudo, Moriya Ohkuma, Somboon Tanasupawat
A Gram-stain positive actinomycete, strain AZ1-19T , isolated from roots of Azadirachta indica A. Juss. var. siamensis Valeton, collected from Chachoengsao province, Thailand, was characterised taxonomically by using a polyphasic approach. Strain AZ1-19T was found to have characteristics consistent with those of members of the genus Micromonospora. The cell wall peptidoglycan of the strain was found to contain meso-diaminopimelic acid. The predominant phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol...
August 30, 2018: Antonie Van Leeuwenhoek
Charles E Deutch
Paenarthrobacter aurescens (formerly called Arthrobacter aurescens) strain TC1 is a high G + C Gram-positive aerobic bacterium that can degrade the herbicide atrazine. Analysis of its genome indicated strain TC1 has the potential to form a bifunctional PutA protein containing L-proline dehydrogenase and L-glutamate-γ-semialdehyde dehydrogenase (L-Δ1 -pyrroline-5-carboxylate dehydrogenase) activities. P. aurescens strain TC1 grew well in minimal media with L-Proline as a supplemental nutrient, the nitrogen source, or the sole carbon and nitrogen source...
August 29, 2018: Antonie Van Leeuwenhoek
Ken-Ichi Kucho, Hiroyuki Tobita, Mari Ikebe, Mitsue Shibata, Akihiro Imaya, Daisuke Kabeya, Tomoyuki Saitoh, Toru Okamoto, Kenji Ono, Kazuhito Morisada
In 1984 at Mt. Ontake in Japan, an earthquake caused a devastating landslide, and as a result, the vegetation on the south slope of the mountain was completely eliminated. In higher elevation (2000 m) areas, revegetation has not yet been completed even 30 years after the landslide. Revegetation progress throughout the area was heterogeneous. In the partially revegetated areas, actinorhizal plant species such as Alnus maximowiczii and Alnus matsumurae have been found. In the present study, we investigated the Frankia communities in the higher-elevation area using sequence analysis of the amplified nifH (dinitrogenase reductase) gene from nodule and soil samples collected in the disturbed region, undisturbed forest, and in the boundary between the disturbed region and the undisturbed forest...
August 28, 2018: Antonie Van Leeuwenhoek
Jong-Hui Kim, Sung Wook Hong, Beom-Young Park, Jae Gyu Yoo, Mi-Hwa Oh
The resident bacteria of the gastrointestinal tract (GIT) and the behaviour of these microbes have been poorly characterised in elk as compared to other ruminant animal species such as sheep and cattle. In addition, most microbial community studies of deer gut have focused on rumen or faeces, while other parts of the GIT such as the small and large intestine have received little attention. To address this issue, the present study investigated the diversity of the GIT bacterial community in elk (Cervus canadensis) by 16S rRNA pyrosequencing analysis...
August 28, 2018: Antonie Van Leeuwenhoek
Fernando Augusto da Silveira, Raphael Hermano S Diniz, Geraldo M S Sampaio, Rogelio L Brandão, Wendel B da Silveira, Ieso M Castro
The pattern of glucose repression in most Kluyveromyces marxianus strains does not correlate with fermentative behaviour; however, glucose repression and fermentative metabolism appear to be linked to the kinetics of sugar uptake. In this work, we show that lactose transport in K. marxianus CCT 7735 by lactose-grown cells is mediated by a low-affinity H+ -sugar symporter. This system is glucose repressed and able to transport galactose with low affinity. We also observed the activity of a distinct lactose transporter in response to raffinose...
August 21, 2018: Antonie Van Leeuwenhoek
Lan-Yu Li, Zi-Wen Yang, Mipeshwaree Devi Asem, Nimaichand Salam, Min Xiao, Dalal Hussien M Alkhalifah, Wael N Hozzein, Guo-Xing Nie, Wen-Jun Li
Strain SYSU D8008T was isolated from a desert sand sample collected from Saudi Arabia. The strain was observed to be Gram-stain positive, non-motile and aerobic. It can grow at 15-37 °C, pH 6.0-10.0 and can tolerant up to 7% (w/v) NaCl. Pairwise comparison of the 16S rRNA gene sequence indicated that strain SYSU D8008T shares high sequence similarities with Georgenia deserti SYSU D8004T (96.8%) and Georgenia halophila YIM 93316T (96.8%). Menaquinone MK-8(H4 ) was detected as the respiratory isoprenologue...
August 21, 2018: Antonie Van Leeuwenhoek
Yu Sato, Hiroyuki Kimura
Haloarcula strains, which are halophilic archaea, harbour two to three copies of 16S rRNA genes (rrsA, rrsB and rrsC) in their genomes. While rrsB and rrsC (rrsBC) show almost identical sequences, rrsA shows 4-6% sequence difference and 1-3% guanine-plus-cytosine content (PGC ) difference compared to rrsBC. Based on the strong correlation between the PGC of 16S rRNA genes and the growth temperatures of the prokaryotes, we hypothesised that high-PGC rrsA and low-PGC rrsBC are expressed at high and low temperatures, respectively...
August 20, 2018: Antonie Van Leeuwenhoek
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