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Antonie Van Leeuwenhoek

Sarah M Curtis, Iulia Norton, Gareth J Everest, Paul R Meyers
An endophytic actinobacterial strain was isolated from a yellowwood tree growing on the slope of Devil's Peak, Cape Town, South Africa. Analysis of the 16S rRNA gene showed that the strain belongs to the genus Kribbella. Phylogenetic analyses using the 16S rRNA gene and multilocus sequence analysis using the concatenated gene sequences of the gyrB, rpoB, relA, recA and atpD genes showed that strain YPL1(T) is closely related to the type strains of Kribbella karoonensis and Kribbella shirazensis. DDH experiments showed that strain YPL1(T) is a distinct genomic species from its close phylogenetic relative, K...
November 17, 2017: Antonie Van Leeuwenhoek
Dan-Yang Wang, Ya-Wen Sun, Jie Liu, De-Chao Zhang
A Gram-stain negative, rod-shaped, aerobic strain, designated YC973(T), was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain YC973(T) is related to the genus Maribacter and has high 16S rRNA gene sequence similarity to Maribacter orientalis KMM 3947(T) (97.6%). The predominant cellular fatty acids were iso-C15:0, iso-C15:1 G and an unidentified fatty acid of equivalent chain-length 13.565. The polar lipid profile contained phosphatidylethanolamine and five unidentified lipids...
November 17, 2017: Antonie Van Leeuwenhoek
Anastasia A Ivanova, Dmitriy A Philippov, Irina S Kulichevskaya, Svetlana N Dedysh
Members of the phylum Planctomycetes were originally described as freshwater bacteria. Most recent studies, however, address planctomycete diversity in other environments colonized by these microorganisms, including marine and terrestrial ecosystems. This study was initiated in order to revisit the specific patterns of planctomycete diversity in freshwater habitats using cultivation-independent approaches. The specific focus was made on planctomycetes associated with Nuphar lutea (L.) Smith, an emergent macrophyte with floating leaves, which is widespread in the Holarctic...
November 17, 2017: Antonie Van Leeuwenhoek
Leonardo Jose Silva, Danilo Tosta Souza, Diego Bonaldo Genuario, Harold Alexander Vargas Hoyos, Suikinai Nobre Santos, Luiz Henrique Rosa, Tiago Domingues Zucchi, Itamar Soares Melo
A novel actinobacterium, designated strain CMAA 1533(T), was isolated from the rhizosphere of Deschampsia antarctica collected at King George Island, Antarctic Peninsula. Strain CMAA 1533(T) was found to grow over a wide range of temperatures (4-28 °C) and pH (4-10). Macroscopically, the colonies were observed to be circular shaped, smooth, brittle and opaque-cream on most of the culture media tested. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMAA 1533(T) belongs to the family Nocardiaceae and forms a distinct phyletic line within the genus Rhodococcus...
November 15, 2017: Antonie Van Leeuwenhoek
Xiaolan Zhang, Defeng Wu, Tengfei Guo, Tingting Ran, Weiwu Wang, Dongqing Xu
ArcAB is a two-component regulatory system that can help bacteria respond to and survive in a changing environment. To identify the function of ArcAB homologues in Serratia marcescens FS14, in-frame deletion mutants of the arcA, arcB and arcAB genes were constructed. Surprisingly, ArcB affects the motility of FS14, but ArcA does not. These results are the reverse of those found in Escherichia coli. Further studies demonstrated that ArcB could promote bacterial motility by activating the synthesis of flagella and particularly by activating the expression of the biosurfactant serrawettin W1...
November 14, 2017: Antonie Van Leeuwenhoek
Dong-Uk Kim, Hyosun Lee, Suyeon Lee, Sooyeon Park, Jung-Hoon Yoon, Lei Zhao, Min-Kyu Kim, Jae-Hyung Ahn, Jong-Ok Ka
A gamma radiation-resistant and pink-to-red pigmented bacterial strain, designated ID1504(T), was isolated from a car air-conditioning system sampled in Korea. The cells were observed to be Gram-stain negative, aerobic, motile with peritrichous flagella and short rod-shaped. Phylogenetically, the strain groups with the members of the genus Deinococcus and exhibits high 16S rRNA gene sequence similarities with Deinococcus arenae SA1(T) (94.0%), Deinococcus actinosclerus BM2(T) (93.9%) and Deinococcus soli N5(T) (93...
November 14, 2017: Antonie Van Leeuwenhoek
Xia Shen, Yu Li, Zhe Zhao, Yi-Fan Han, Wen-Wu Zhang, Xiao-Yun Yu, Chong-Ya Zhang, Cong Sun, Min Wu
A Gram-stain negative, aerobic, motile and ovoid- to rod-shaped bacteria strain, designated XC0140(T), was isolated from soil samples near the sewage treatment tank of a chemical factory in Zhejiang Province, China, and subjected to polyphasic taxonomic investigation. Strain XC0140(T) grew at 10-37 °C and pH 6.0-9.0 (optimum, 35 °C and pH 7.5) and with 0-17% (w/v) NaCl (optimum, 1%). According to phylogenetic analysis based on 16S rRNA gene sequences, strain XC0140(T) was assigned to the genus Pararhizobium with high 16S rRNA gene sequence similarity of 95...
November 13, 2017: Antonie Van Leeuwenhoek
Anastasia A Ivanova, Carl-Eric Wegner, Yongkyu Kim, Werner Liesack, Svetlana N Dedysh
Members of the phylum Planctomycetes are common inhabitants of northern Sphagnum-dominated wetlands. Evidence is accumulating that, in these environments, some planctomycetes may be involved in degrading polymeric organic matter. The experimental data, however, remain scarce due to the low number of characterized representatives of this phylum. In a previous study, we used metatranscriptomics to assess the activity response of peat-inhabiting microorganisms to biopolymers abundantly present in native peat. The community responses to cellulose, xylan, pectin, and chitin availability were analysed relative to unamended controls...
November 13, 2017: Antonie Van Leeuwenhoek
Yun Wang, Wei-Xun Chunyu, Gang-Qiang Jiang, Ling Huang, Bo Zhang, Yang Ji, Zhang-Gui Ding, Min Yin, Shu-Kun Tang
A Gram-positive, non-motile, asporogenous and aerobic bacterium, designated YIM 98012(T), was isolated from a salt lake in China. Strain YIM 98012(T) was found to be catalase and oxidase positive. Optimal growth of strain YIM 98012(T) was observed at 37 °C and pH 7.0 and it was found to grow in the presence of 5-20% (w/v) NaCl (optimum 10% NaCl). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel strain is affiliated with the family Bacillaceae of the phylum Firmicutes and that it shares high (94...
November 13, 2017: Antonie Van Leeuwenhoek
Michel Diouf, Vincent Hervé, Philippe Mora, Alain Robert, Sophie Frechault, Corinne Rouland-Lefèvre, Edouard Miambi
Studies on termite symbiosis have revealed that significant symbiont lineages are maintained across generations. However, most studies have focused only on the worker caste. Little is known about the gut microbiota of reproductives, the most probable vectors for transmitting these lineages to offspring. Using 16S rRNA gene-based Illumina MiSeq sequencing, we compared the gut microbiota of swarming alates of the higher termite Nasutitermes arborum with those of their nestmates from the parental colony. The OTU-based alpha diversity indices showed that the gut microbiota of the alates was at least as diverse as those of non-reproductive adults...
November 10, 2017: Antonie Van Leeuwenhoek
Priscila Caroline Thiago Dobbler, Álvaro Macedo Laureano, Deise Schroder Sarzi, Ehidy Rocio Peña Cañón, Geferson Fernando Metz, Anderson Santos de Freitas, Beatriz Midori Takagaki, Cristiane Barbosa D Oliveira, Victor Satler Pylro, André Carlos Copetti, Filipe Victoria, Marc Redmile-Gordon, Daniel Kumazawa Morais, Luiz Fernando Wurdig Roesch
Humans distribute a wide range of microorganisms around building interiors, and some of these are potentially pathogenic. Recent research established that humans are the main drivers of the indoor microbiome and up to now significant literature has been produced about this topic. Here we analyzed differences in bacterial composition between men's and women's restrooms and other common areas within the same public building. Bacterial DNA samples were collected from restrooms and halls of a three-floor building from the Federal University of Pampa, RS, Brazil...
November 10, 2017: Antonie Van Leeuwenhoek
Camila S Varize, Raquel M Cadete, Lucas D Lopes, Renata M Christofoleti-Furlan, Marc-André Lachance, Carlos A Rosa, Luiz C Basso
Two strains of a novel yeast species were isolated from rotting wood of an ornamental tree (purple quaresmeira, Tibouchina granulosa, Melastomataceae) in an Atlantic Rainforest area in Brazil. Analysis of the sequences of the internal transcribed spacer (ITS-5.8S) region and the D1/D2 domains of the large subunit rRNA gene showed that this species belongs to the Spathaspora clade, and is phylogenetically related to Spathaspora brasiliensis, Candida materiae and Sp. girioi. The novel species ferments D-xylose, producing ethanol, with amounts between 3...
November 9, 2017: Antonie Van Leeuwenhoek
Edgar Guevara-Avendaño, Joseph D Carrillo, Cedric Ndinga-Muniania, Kevin Moreno, Alfonso Méndez-Bravo, José A Guerrero-Analco, Akif Eskalen, Frédérique Reverchon
Plant rhizobacteria have been successfully used as biocontrol agents against fungal phytopathogens. However, their potential to control two important avocado diseases, namely Fusarium dieback (FD) and Phytophthora root rot (PRR), has been poorly studied. FD is an emerging disease triggered by fungi associated with two ambrosia beetle species (Euwallacea fornicatus species complex), while PRR is caused by Phytophthora cinnamomi, a soil-borne oomycete. In the present work, the antifungal activity of bacteria isolated from avocado rhizosphere was tested in dual culture assays against Fusarium euwallaceae, Graphium euwallaceae and Graphium sp...
November 9, 2017: Antonie Van Leeuwenhoek
Hao Wu, Bin Liu, Xiaoyun Ou, Shangli Pan, Yuanyuan Shao, Fuchang Huang
During an investigation exploring potential sources of novel thermophilic species and natural products, a novel thermophilic and alkaliphilic actinomycete with alkaline cellulase producing ability, designated strain 4-2-13(T), was isolated from soil of a tropical rainforest in Xishuangbanna, Yunnan province, China. The morphological and chemotaxonomic characteristics of strain 4-2-13(T) are consistent with those of the members of the genus Streptomyces. The strain forms extensively branched aerial mycelia and substrate mycelia...
November 7, 2017: Antonie Van Leeuwenhoek
Renata Medina-Silva, Rafael R Oliveira, Fernanda J Trindade, Luiz G A Borges, Taiz L Lopes Simão, Adolpho H Augustin, Fernanda P Valdez, Marcelo J Constant, Carolina L Simundi, Eduardo Eizirik, Claudia Groposo, Dennis J Miller, Priscila Reis da Silva, Adriano R Viana, João M M Ketzer, Adriana Giongo
As the depth increases and the light fades in oceanic cold seeps, a variety of chemosynthetic-based benthic communities arise. Previous assessments reported polychaete annelids belonging to the family Siboglinidae as part of the fauna at cold seeps, with the 'Vestimentifera' clade containing specialists that depend on microbial chemosynthetic endosymbionts for nutrition. Little information exists concerning the microbiota of the external portion of the vestimentiferan trunk wall. We employed 16S rDNA-based metabarcoding to describe the external microbiota of the chitin tubes from the vestimentiferan Escarpia collected from a chemosynthetic community in a cold seep area at the southwestern Atlantic Ocean...
November 6, 2017: Antonie Van Leeuwenhoek
Marilize le Roes-Hill, Alaric Prins, Paul R Meyers
As part of an antibiotic screening program, an actinobacterium, strain HMC13(T), was isolated from soil collected from the banks of the Gamka River, Western Cape Province, South Africa. The isolate was found to produce branched mycelia that differentiated into spiral spore chains with spiny spores. 16S rRNA gene sequence analysis showed the strain to be closely related to Streptomyces caelestis NRRL 2418(T) (99.72%) and Streptomyces azureus NBRC 12744(T) (99.51%). Chemotaxonomic analyses confirmed the classification of the strain as a member of the genus Streptomyces: LL-DAP in the peptidoglycan, no diagnostic sugars in the whole cell sugar pattern, dominant menaquinones including MK9(H8), MK9(H6), and the polar lipids detected included phosphatidylethanolamine...
November 6, 2017: Antonie Van Leeuwenhoek
Srinivasan Ramanathan, Durgadevi Ravindran, Kannappan Arunachalam, Veera Ravi Arumugam
The aim of this study was to evaluate the anti-biofilm and anti-virulence properties of petroselinic acid (PSA) against the environmental pathogen Serratia marcescens. PSA significantly inhibited the quorum sensing (QS)-dependent virulence factors such as prodigiosin, protease productions, and biofilm formation in S. marcescens. The antibiofilm potential of PSA was also confirmed through light, confocal laser scanning, and scanning electron microscopic analyses. Furthermore, PSA effectively inhibited the biofilm-related phenomena such as exopolysaccharide production, hydrophobicity production, swimming, and swarming motility without affecting the bacterial growth...
November 3, 2017: Antonie Van Leeuwenhoek
Sheng Wang, Biyao Mao, Mingxi Wu, Jingjing Liang, Le Deng
Biofilms are bacterial communities consisting of numerous extracellular polymeric substances. Infections caused by biofilm-forming bacteria are considered to be a major threat to health security and so novel approaches to control biofilm are of importance. Aptamers are single-strand nucleic acid molecules that have high selectivity to their targets. Single-walled carbon nanotubes (SWNTs) are common nanomaterials and have been shown to be toxic to bacterial biofilms. The aim of this study was to test whether an aptamer could play a role as targeting agents to enhance the efficiency of anti-biofilm agents...
November 2, 2017: Antonie Van Leeuwenhoek
C Douanla-Meli, J-G Unger, E Langer
Colletotrichum cliviae is a fungal species reported both as pathogen and endophyte with broad geographical distribution. Some purported isolates of this species have been assigned to different taxa, including Colletotrichum aracearum, Colletotrichum orchidearum and Colletotrichum. sichuanensis, for which a preliminary analysis of extensive multilocus (ACT, GAPDH, ITS, TUB2) data in this study revealed high sequence similarity with C. cliviae. We further reassessed the species delineation by using the coalescent method of the generalized mixed Yule-coalescent (GMYC) and Poisson Tree Processes (PTP)...
November 1, 2017: Antonie Van Leeuwenhoek
Dino van Dissel, Gilles P van Wezel
Actinobacteria are prolific producers of secondary metabolites and industrially relevant enzymes. Growth of these mycelial micro-organisms in small culture volumes is challenging due to their complex morphology. Since morphology and production are typically linked, scaling down culture volumes requires better control over morphogenesis. In larger scale platforms, ranging from shake flasks to bioreactors, the hydrodynamics play an important role in shaping the morphology and determining product formation. Here, we report on the effects of agitation on the mycelial morphology of Streptomyces lividans grown in microtitre plates...
November 1, 2017: Antonie Van Leeuwenhoek
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