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Antonie Van Leeuwenhoek

Paula Arrúa Day, María S Villalba, O Marisa Herrero, Luz Alejandra Arancibia, Héctor M Alvarez
We investigated previously under explored cold aquatic environments of Andean Patagonia, Argentina. Oily sheens similar to an oil spill are frequently observed at the surface of water in creeks and small ponds in these places. Chemical analysis of a water sample revealed the occurrence of high concentrations of iron and the presence of a free insoluble indigoidine-derived pigment. A blue pigment-producing bacterium (strain EB) was isolated from the water sample and identified as Vogesella sp. by molecular analysis...
December 3, 2016: Antonie Van Leeuwenhoek
Khaoula Bouznada, Noureddine Bouras, Salim Mokrane, Fawzia Chaabane Chaouch, Abdelghani Zitouni, Gabriele Pötter, Cathrin Spröer, Hans-Peter Klenk, Nasserdine Sabaou
The taxonomic position of a new Saccharothrix strain, designated MB46(T), isolated from a Saharan soil sample collected in Mzab region (Ghardaïa province, South Algeria) was established following a polyphasic approach. The novel microorganism has morphological and chemical characteristics typical of the members of the genus Saccharothrix and formed a phyletic line at the periphery of the Saccharothrix espanaensis subcluster in the 16S rRNA gene dendrograms. Results of the 16S rRNA gene sequence comparisons revealed that strain MB46(T) shares high degrees of similarity with S...
December 3, 2016: Antonie Van Leeuwenhoek
Ildar I Mustakhimov, Olga N Rozova, Natalia P Solntseva, Valentina N Khmelenina, Alexander S Reshetnikov, Yuri A Trotsenko
Aerobic bacteria utilizing methane as the carbon and energy source do not use sugars as growth substrates but possess the gene coding for glucokinase (Glk), an enzyme converting glucose into glucose 6-phosphate. Here we demonstrate the functionality and properties of Glk from an obligate methanotroph Methylomicrobium alcaliphilum 20Z. The recombinant Glk obtained by heterologous expression in Escherichia coli was found to be close in biochemical properties to other prokaryotic Glks. The homodimeric enzyme (2 × 35 kDa) catalyzed ATP-dependent phosphorylation of glucose and glucosamine with nearly equal activity, being inhibited by ADP (K i = 2...
December 3, 2016: Antonie Van Leeuwenhoek
Karima Hezbri, Imen Nouioui, Manfred Rohde, Peter Schumann, Maher Gtari, Hans-Peter Klenk, Maria Del Carmen Montero-Calasanz, Faten Ghodhbane-Gtari
The taxonomic position of an actinobacterial isolate, designated strain BMG 822(T), isolated from limestone from the Amphitheater of El Jem (Coliseum Thysdrus), Tunisia, was established using a polyphasic approach. Strain BMG 822(T) was found to grow well at 30 °C and pH 6.5-8.0, and to be coral-coloured, Gram-positive, catalase and oxidase negative. Whole cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, glucose, galactose and ribose. The phospholipids detected were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, an unidentified glycophospholipid and six unidentified phospholipids...
November 28, 2016: Antonie Van Leeuwenhoek
Mirzeta Hadžibrahimović, Desanka Sužnjević, Ferenc Pastor, Tijana Cvetić Antić, Milan Žižić, Joanna Zakrzewska, Miroslav Živić
The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 µmol/gFW, and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation...
November 28, 2016: Antonie Van Leeuwenhoek
Débora Souza Collares Maia Castelo-Branco, Aline Lobão da Silva, Frederico Ozanan Barros Monteiro, Glaucia Morgana de Melo Guedes, Jamille Alencar Sales, Jonathas Sales de Oliveira, José Erisvaldo Maia Junior, Stefânia Araújo Miranda, José Júlio Costa Sidrim, Lucas Pereira de Alencar, Raimunda Sâmia Nogueira Brilhante, Rossana de Aguiar Cordeiro, Tereza de Jesus Pinheiro Gomes Bandeira, Waldemiro de Aquino Pereira Neto, Marcos Fábio Gadelha Rocha
The present study aimed at evaluating the role of captive scarlet ibises (Eudocimus ruber) and their environment as reservoirs of Aeromonas spp. and Plesiomonas spp., and analyzing the in vitro antimicrobial susceptibility and virulence of the recovered bacterial isolates. Thus, non-lactose and weak-lactose fermenting, oxidase positive Gram-negative bacilli were recovered from cloacal samples (n = 30) of scarlet ibises kept in a conservational facility and from water samples (n = 30) from their environment...
November 24, 2016: Antonie Van Leeuwenhoek
Zhi Huang, Fei Zhao, Yong-Hui Li
A novel strain, designated strain CSA42(T), was isolated from the tomb of emperor Yang of Sui in Yangzhou, Jiangsu province, China. Strain CSA42(T) was observed to be Gram-stain positive, strictly aerobic, rod-shaped, spore-forming and motile. The optimum conditions for growth were found to be 30 °C, pH 8.0 and without NaCl. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed strain CSA42(T) to be closely related to Paenibacillus larvae DSM 7030(T) (94.7%), Paenibacillus doosanensis CAU 1055(T) (94...
November 24, 2016: Antonie Van Leeuwenhoek
Alaa Al-Seraih, Yanath Belguesmia, John Baah, Sabine Szunerits, Rabah Boukherroub, Djamel Drider
Enterococcus faecalis B3A-B3B produces the bacteriocin B3A-B3B with activity against Listeria monocytogenes, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium perfringens, but apparently not against fungi or Gram-negative bacteria, except for Salmonella Newport. B3A-B3B enterocin has two different nucleotides but similar amino acid composition to the class IIb MR10A-MR10B enterocin. B3A-B3B consists of two peptides of predicted molecular mass of 5176.31 Da (B3A) and 5182...
November 22, 2016: Antonie Van Leeuwenhoek
Yi-Rui Yin, Qing-Wen Hu, Wen-Dong Xian, Feng Zhang, En-Min Zhou, Hong Ming, Min Xiao, Xiao-Yang Zhi, Wen-Jun Li
A xylanase gene (TrXyn10) from Thermoactinospora rubra YIM 77501(T) was cloned and expressed in Escherichia coli. The amino acid sequence displayed 78% homology with Microbispora mesophila xylanase (WP_062413927.1). The recombinant xylanase (TrXyn10), with MW 46.1 kDa, could hydrolyse beechwood, birchwood and oatspelt xylan. Based on the sequence, enzymatic properties and tertiary structure of the protein, TrXyn10 belongs to glycoside hydrolase family 10 (GH10). The optimal pH and temperature for the recombinant enzyme were determined to be 7...
November 19, 2016: Antonie Van Leeuwenhoek
Chang-Feng Xu, Long Zhang, Jun-Wei Huang, Kai Chen, Shun-Peng Li, Jian-Dong Jiang
An aerobic, Gram-stain negative, short rod-shaped, asporogenous, non-motile bacterium designated strain NK8(T) was isolated from a chlorobenzoate contaminated soil in China. Strain NK8(T) was observed to grow optimally at pH 7.0, 30 °C and in the absence of NaCl in LB medium. The G + C content of the total DNA of strain NK8(T) was found to be 65.5 mol%. The 16S rRNA gene sequence of strain NK8(T) showed high similarity to that of Aquamicrobium aerolatum Sa14(T) (97.3%), followed by Aquamicrobium lusatiense S1(T) (96...
November 17, 2016: Antonie Van Leeuwenhoek
Shashank Kumar Mishra, Mohammad Haneef Khan, Sankalp Misra, Vijay Kant Dixit, Praveen Khare, Suchi Srivastava, Puneet Singh Chauhan
Soil bacteria may have properties of plant growth promotion but not be sufficiently beneficial for plants under stress conditions. This challenge has led researchers to extend their searches into extreme environments for potential soil bacteria with multiple plant beneficial traits as well as abiotic stress tolerance abilities. In the current study, an attempt was made to evaluate soil bacteria from an extreme environment, volcano soils, based on plant growth promoting and abiotic stress mitigating characteristics...
November 16, 2016: Antonie Van Leeuwenhoek
Li-Qiang Yang, Lili Tang, Lan Liu, Nimaichand Salam, Wen-Jun Li, Xiaowei Liu, Guangzhi Jin, Nianzhi Jiao, Yongyu Zhang
A Gram-stain negative, ovoid or short rod-shaped, aerobic and non-motile bacterial strain, designated J82(T), was isolated from a seawater sample collected from the coast of Yellow Sea in Qingdao, China. The strain grew at salinities of 1.0-6.0% (w/v) NaCl (optimum, 2.5%). Growth occurred at pH 6.0-8.0 (optimum, pH 7.0) and 10-42 °C (optimum, 28-30 °C). The genomic DNA G + C content was determined to be 57.5 mol%. Q-10 was detected as the respiratory quinone. The major fatty acid (>10%) was Summed feature 8 (C18:1 ω7c and/or C18:1 ω6c)...
November 16, 2016: Antonie Van Leeuwenhoek
Annechien Plat, Anneke Kuipers, Joe Crabb, Rick Rink, Gert N Moll
The lantibiotic nisin is produced by Lactococcus lactis as a precursor peptide comprising a 23 amino acid leader peptide and a 34 amino acid post-translationally modifiable core peptide. We previously demonstrated that the conserved FNLD part of the leader is essential for intracellular enzyme-catalyzed introduction of lanthionines in the core peptide and also for transporter-mediated export, whereas other positions are subject to large mutational freedom. We here demonstrate that, in the absence of the extracellular leader peptidase, NisP, export of precursor nisin via the modification and transporter enzymes, NisBTC, is strongly affected by multiple substitutions of the leader residue at position -2, but not by substitution of positions in the vicinity of this site...
November 10, 2016: Antonie Van Leeuwenhoek
Ronel Roberts, Gerhard Pietersen
The phloem limited bacterium 'Candidatus Liberibacter africanus' is associated with citrus greening disease in South Africa. This bacterium has been identified solely from commercial citrus in Africa and the Mascarene islands, and its origin may lie within an indigenous rutaceous host from Africa. Recently, in determining whether alternative hosts of Laf exist amongst the indigenous rutaceous hosts of its triozid vector, Trioza erytreae, three novel subspecies of Laf were identified i.e. 'Candidatus Liberibacter africanus subsp...
November 9, 2016: Antonie Van Leeuwenhoek
Imen Nouioui, Faten Ghodhbane-Gtari, Maria Del Carmen Montero-Calasanz, Manfred Rohde, Louis S Tisa, Maher Gtari, Hans-Peter Klenk
Strain EuI1c(T) is the first actinobacterial endophyte isolated from Elaeagnus umbellata that was shown to be infective on members of Elaeagnaceae and Morella but lacking the ability to form effective root nodules on its hosts. The strain can be easily distinguished from strains of other Frankia species based on its inability to produce vesicles, the specialized thick-walled structures where nitrogen fixation occurs. Chemotaxonomically, strain EuI1c(T) contains phosphatidylinositol, diphosphatidylglycerol, two glycophospholipids and phosphatidylglycerol as phospholipids...
November 9, 2016: Antonie Van Leeuwenhoek
Ping Mo, Yi-Zun Yu, Jia-Rong Zhao, Jian Gao
An actinomycete strain, designated strain LUSFXJ(T), was isolated from a soil sample obtained near the Xiangtan Manganese Mine, Central-South China and characterised using a polyphasic taxonomic approach. The 16S rRNA gene sequence-based phylogenetic analysis indicated that this strain belongs to the genus Streptomyces. The DNA-DNA relatedness between this strain and two closely related type strains, Streptomyces echinatus CGMCC 4.1642(T) and Streptomyces lanatus CGMCC 4.137(T), were 28.7 ± 0.4 and 19.9 ± 2...
November 8, 2016: Antonie Van Leeuwenhoek
Fawzia Chaabane Chaouch, Noureddine Bouras, Salim Mokrane, Khaoula Bouznada, Abdelghani Zitouni, Gabriele Pötter, Cathrin Spröer, Hans-Peter Klenk, Nasserdine Sabaou
A filamentous actinobacterium, designated strain PM3(T), was isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (South-West Algeria). A polyphasic taxonomic study was carried out to establish the status of strain PM3(T). The isolate was found to have morphological and chemotaxonomical properties associated with members of the genus Planomonospora. The new isolated microorganism developed cylindrical sporangia arranged in double parallel rows on aerial mycelium, each one containing a motile single sporangiospore...
November 1, 2016: Antonie Van Leeuwenhoek
Priyanka Singh, Hina Singh, Yeon-Ju Kim, Deok-Chun Yang
A novel strain, DCY108(T) was isolated from soil of a Panax ginseng field, Yeoncheon province (38°04'N 126°57'E), Republic of Korea. Strain DCY108(T) is Gram-negative, non-motile, non-flagellate, rod-shaped, and aerobic. The bacterium grows optimally at 25-30 °C, pH 6.5-7.0 and 1 % NaCl. Phylogenetically, strain DCY108(T) is closely related to Pedobacter jejuensis JCM 18824(T), Pedobacter aquatilis JCM 13454(T), Pedobacter kyungheensis LMG 26577(T) and the type strain of the genus Pedobacter heparinus DSM 2366(T)...
October 31, 2016: Antonie Van Leeuwenhoek
Tríona Marie Dooley-Cullinane, Catherine O'Reilly, Lee Coffey
Aldoxime dehydratase catalyses the conversion of aldoximes to their corresponding nitriles. Utilization of the aldoxime-nitrile metabolising enzyme pathway can facilitate the move towards a greener chemistry. In this work, a real-time PCR assay was developed for the detection of aldoxime dehydratase genes in aldoxime/nitrile metabolising microorganisms which have been purified from environmental sources. A conventional PCR assay was also designed allowing gene confirmation via sequencing. Aldoxime dehydratase genes were identified in 30 microorganisms across 11 genera including some not previously shown to harbour the gene...
October 27, 2016: Antonie Van Leeuwenhoek
Tobias Eisenberg, Karin Riße, Nicole Schauerte, Christina Geiger, Jochen Blom, Holger C Scholz
A pleomorphic Gram-negative, motile coccobacillus was isolated from the gills of a wild-caught bluespotted ribbontail ray after its sudden death during quarantine. Strain 141012304 was observed to grow aerobically, to be clearly positive for cytochrome oxidase, catalase, urease and was initially identified as "Brucella melitensis" or "Ochrobactrum anthropi" by Matrix-assisted laser desorption/ionization-time of flight mass spectrometry and VITEK2-compact(®), respectively. Affiliation to the genus Brucella was confirmed by bcsp31 and IS711 PCR as well as by Brucella species-specific multiplex PCR, therein displaying a characteristic banding pattern recently described for Brucella strains obtained from amphibian hosts...
October 26, 2016: Antonie Van Leeuwenhoek
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