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Antonie Van Leeuwenhoek

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https://www.readbyqxmd.com/read/28748290/selection-for-novel-acid-tolerant-desulfovibrio-spp-from-a-closed-transbaikal-mine-site-in-a-temporal-ph-gradient-bioreactor
#1
Dmitry V Antsiferov, Tatiana S Fyodorova, Anastasia A Kovalyova, Anastasia Lukina, Yulia A Frank, Marat R Avakyan, David Banks, Olli H Tuovinen, Olga V Karnachuk
Almost all the known isolates of acidophilic or acid-tolerant sulphate-reducing bacteria (SRB) belong to the spore-forming genus Desulfosporosinus in the Firmicutes. The objective of this study was to isolate acidophilic/acid-tolerant members of the genus Desulfovibrio belonging to deltaproteobacterial SRB. The sample material originated from microbial mat biomass submerged in mine water and was enriched for sulphate reducers by cultivation in anaerobic medium with lactate as an electron donor. A stirred tank bioreactor with the same medium composition was inoculated with the sulphidogenic enrichment...
July 26, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28748289/genetic-recombinational-events-in-prokaryotes-and-their-viruses-insight-into-the-study-of-evolution-and-biodiversity
#2
REVIEW
Jean-Pierre Gratia
The exact meaning of sexual reproduction and the precise evolutionary period at which recombination first took place remains the subject of intense debates. Despite some unity in biochemical organisation of genetic recombination, a plethora of mechanisms are found to exist in microbes and their viruses. Some routes used by viruses bypass barriers to genetic heterology and provide bacteria with genes conferring a selective advantage, and some contribute to genome enlargement. The present review aims at highlighting the diversity of such mechanisms with a particular focus on spontaneous zygogenesis (or Z-mating)...
July 26, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28748288/talaromyces-sayulitensis-acidiella-bohemica-and-penicillium-citrinum-in-brazilian-oil-shale-by-products
#3
Kelly C G P de Goes, Josué J da Silva, Gisele M Lovato, Beatriz T Iamanaka, Fernanda P Massi, Diva S Andrade
Fine shale particles and retorted shale are waste products generated during the oil shale retorting process. These by-products are small fragments of mined shale rock, are high in silicon and also contain organic matter, micronutrients, hydrocarbons and other elements. The aims of this study were to isolate and to evaluate fungal diversity present in fine shale particles and retorted shale samples collected at the Schist Industrialization Business Unit (Six)-Petrobras in São Mateus do Sul, State of Paraná, Brazil...
July 26, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28733844/polyamine-binding-protein-potd2-is-required-for-stress-tolerance-and-virulence-in-actinobacillus-pleuropneumoniae
#4
Zhuang Zhu, Qin Zhao, Yu Zhao, Fei Zhang, Xintian Wen, Xiaobo Huang, Yiping Wen, Rui Wu, Qigui Yan, Yong Huang, Xiaoping Ma, Xinfeng Han, Sanjie Cao
Actinobacillus pleuropneumoniae is the cause of porcine contagious pleuropneumonia, which is one of the most important respiratory diseases in swine and causes huge economic losses in the swine industry. PotD, a polyamine-binding protein, has been well characterised in many pathogens of humans and animals. In this study, a ΔpotD2 mutant of A. pleuropneumoniae strain MS71 (serovar 1) was constructed successfully by homologous recombination. Growth curves of different strains showed that the growth of the ΔpotD2 mutant was affected greatly in the logarithmic phase compared with that of parental strain...
July 21, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28730371/idiomarina-andamanensis-sp-nov-an-alkalitolerant-bacterium-isolated-from-andaman-sea-water
#5
Sherin Zachariah, Subrata K Das
Two closely related aerobic, Gram-negative rod shaped bacteria (strain W5(T) and W3) were isolated from Andaman Sea. Heterotrophic growth on marine agar was observed at 15-45 °C and pH 6-10. Strain W5(T) showed maximum 16S rRNA sequence similarity of 99.58% with Idiomarina marina JCM 15083(T). DNA fingerprinting analysis by ERIC-REP PCR, PFGE and MLSA revealed differences in banding patterns, also DNA-DNA hybridization values were well below 70% confirming W5(T) to be a new species. DNA G+C content was 46...
July 20, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28730370/the-n-terminal-and-central-domain-of-colicin-a-enables-phage-lysin-to-lyse-escherichia-coli-extracellularly
#6
Guangmou Yan, Jianfang Liu, Qiang Ma, Rining Zhu, Zhimin Guo, Chencheng Gao, Shuang Wang, Ling Yu, Jingmin Gu, Dongliang Hu, Wenyu Han, Rui Du, Junling Yang, Liancheng Lei
Multidrug-resistant Escherichia coli has seriously threatened antibiotic resources and international public health. Bacteriophage lysin preparations have been widely considered as valid agents for solving multidrug resistances. Many lysins have been derived to treat diseases caused by Gram-positive bacteria, but only a few lysin preparations have been found that successively treat diseases caused by Gram-negative bacteria. The outer membrane of Gram-negative bacteria effectively blocks the interactions between peptidoglycan in the periplasmic space and bacteriophage lysins, which therefore hampers the antimicrobial effects of bacteriophage lysins...
July 20, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28726125/nitrogen-assimilation-in-denitrifier-bacillus-azotoformans-lmg-9581-t
#7
Yihua Sun, Paul De Vos, Anne Willems
Until recently, it has not been generally known that some bacteria can contain the gene inventory for both denitrification and dissimilatory nitrate (NO3(-))/nitrite (NO2(-)) reduction to ammonium (NH4(+)) (DNRA). Detailed studies of these microorganisms could shed light on the differentiating environmental drivers of both processes without interference of organism-specific variation. Genome analysis of Bacillus azotoformans LMG 9581(T) shows a remarkable redundancy of dissimilatory nitrogen reduction, with multiple copies of each denitrification gene as well as DNRA genes nrfAH, but a reduced capacity for nitrogen assimilation, with no nas operon nor amtB gene...
July 19, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28721507/the-diversity-and-antibiotic-properties-of-actinobacteria-associated-with-endemic-deepwater-amphipods-of-lake-baikal
#8
Eugenii S Protasov, Denis V Axenov-Gribanov, Yuriy V Rebets, Irina V Voytsekhovskaya, Bogdan T Tokovenko, Zhanna M Shatilina, Andriy N Luzhetskyy, Maxim A Timofeyev
The emergence of pathogenic bacteria resistant to antibiotics increases the need for discovery of new effective antimicrobials. Unique habitats such as marine deposits, wetlands and caves or unexplored biological communities are promising sources for the isolation of actinobacteria, which are among the major antibiotic producers. The present study aimed at examining cultivated actinobacteria strains associated with endemic Lake Baikal deepwater amphipods and estimating their antibiotic activity. We isolated 42 actinobacterial strains from crustaceans belonging to Ommatogammarus albinus and Ommatogammarus flavus...
July 18, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28698910/erratum-to-structural-characterization-of-an-all-aminosugar-containing-capsular-polysaccharide-from-colwellia-psychrerythraea-34h
#9
Angela Casillo, Jonas Ståhle, Ermenegilda Parrilli, Filomena Sannino, Daniel E Mitchell, Giuseppina Pieretti, Matthew I Gibson, Gennaro Marino, Rosa Lanzetta, Michelangelo Parrilli, Göran Widmalm, Maria L Tutino, Maria M Corsaro
No abstract text is available yet for this article.
July 11, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28695409/selection-and-validation-of-reference-genes-for-rt-qpcr-indicates-that-juice-of-sugarcane-varieties-modulate-the-expression-of-c-metabolism-genes-in-the-endophytic-diazotrophic-herbaspirillum-rubrisubalbicans-strain-hcc103
#10
Valéria Polese, Cleiton de Paula Soares, Paula Renata Alves da Silva, Jean Luiz Simões-Araújo, José Ivo Baldani, Marcia Soares Vidal
Quantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources...
July 10, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28695408/a-gyrb-oligonucleotide-microarray-for-the-specific-detection-of-pathogenic-legionella-and-three-legionella-pneumophila-subsp
#11
Daoyi Xi, Yan Dou, Wei Ren, Shuang Yang, Lu Feng, Boyang Cao, Lei Wang
Among the 50 species and 70 serogroups of Legionella identified, Legionella pneumophila, comprising three subsp. (subsp. pneumophila, subsp. fraseri, and subsp. pasculleii), is recognized as the major cause of epidemic legionellosis. Rapid and reliable assays to identify pathogenic Legionella spp., and the three L. pneumophila subsp. in particular, are in great demand. In this study, we analyzed the gyrB genes of eleven Legionella spp. and subsp., comprising L. anisa, L. bozemanii, L. dumoffii, L. feeleii, L...
July 10, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28687978/two-new-leptographium-spp-reveal-an-emerging-complex-of-hardwood-infecting-species-in-the-ophiostomatales
#12
Robert Jankowiak, Beata Strzałka, Piotr Bilański, Riikka Linnakoski, Truls Aas, Halvor Solheim, Małgorzata Groszek, Z Wilhelm de Beer
Species of Leptographium are generally characterized by mononematous conidiophores and are commonly associated with bark beetles and weevils. These species are responsible for sapstain and in some cases serious diseases on a range of primarily coniferous trees. In comparison with coniferous trees, the occurrence of Leptographium species on hardwood trees has been poorly studied in Europe. During a survey of ophiostomatoid fungi on various tree species in Norway and Poland, three unusual species, which fit the broader morphological description of Leptographium spp...
July 7, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28674761/a-simple-and-efficient-method-for-successful-gene-silencing-of-hspa1-in-trametes-hirsuta-ah28-2
#13
Jingjing Wang, Yanyan Chen, Yanqun Dong, Wei Fang, Zemin Fang, Yazhong Xiao
Trametes sp. are among the most extensively studied basidiomycetes due to their importance in biotechnology. However, they are difficult to genetically modify. For instance, the low incidence of homologous integration hampers gene inactivation. To overcome this, we here constructed two post-transcriptional gene-silencing vectors that contain an antisense and a RNA interference mediating hairpin sequence, respectively. These vectors were used to knock down transcription of the heat shock protein 70 family gene HspA1...
July 3, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28668995/partial-structure-and-immunological-properties-of-lipopolysaccharide-from-marine-derived-pseudomonas-stutzeri-kmm-226
#14
Maxim S Kokoulin, Ekaterina V Sokolova, Yuriy N Elkin, Lyudmila A Romanenko, Valery V Mikhailov, Nadezhda A Komandrova
The partial structure and immunology of the lipopolysaccharide (LPS) of Pseudomonas stutzeri KMM 226, a bacterium isolated from a seawater sample collected at a depth of 2000 m, was characterised. The O-polysaccharide was built up of disaccharide repeating units constituted by L-Rhap and D-GlcpNAc: →2)-α-L-Rhap-(1→3)-α-D-GlcpNAc-(1→. The structural analysis of the lipid A showed a mixture of different species. The major species were hexa-acylated and penta-acylated lipids A, bearing the 12:0(3-OH) in amide linkage and 10:0(3-OH) in ester linkage, while the secondary fatty acids were present only as 12:0...
July 1, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28647822/devosia-nitraria-sp-nov-a-novel-species-isolated-from-the-roots-of-nitraria-sibirica-in-china
#15
Lin Xu, Yong Zhang, Nicholas Read, Shuangjiang Liu, Ville-Petri Friman
An aerobic, Gram-stain negative, short rod-shaped and motile strain, 36-5-1(T), was isolated from the roots of Nitraria sibirica in Zhangye city, Gansu province, north-west of China. Phylogenetic analysis based on the 16S rRNA gene sequence and two housekeeping genes (glnA and atpD) indicated that the strain represents a novel species closely related to the Devosia, Rhizobium and Devosia genera with 98.3, 96.2 and 91.1% similarities, respectively. The strain 36-5-1(T) contained Q-10 as the predominant ubiquinone and 16:0 (36...
June 24, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28639147/rethinking-cell-cycle-dependent-gene-expression-in-schizosaccharomyces-pombe
#16
Stephen Cooper
Three studies of gene expression during the division cycle of Schizosaccharomyces pombe led to the proposal that a large number of genes are expressed at particular times during the S. pombe cell cycle. Yet only a small fraction of genes proposed to be expressed in a cell-cycle-dependent manner are reproducible in all three published studies. In addition to reproducibility problems, questions about expression amplitudes, cell-cycle timing of expression, synchronization artifacts, and the problem with methods for synchronizing cells must be considered...
June 21, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28631172/glutamine-a-major-player-in-nitrogen-catabolite-repression-in-the-yeast-dekkera-bruxellensis
#17
Danielli Batista Bezerra Cajueiro, Denise Castro Parente, Fernanda Cristina Bezerra Leite, Marcos Antonio de Morais Junior, Will de Barros Pita
In the present work we studied the expression of genes from nitrogen central metabolism in the yeast Dekkera bruxellensis and under regulation by the Nitrogen Catabolite Repression mechanism (NCR). These analyses could shed some light on the biological mechanisms involved in the adaptation and survival of this yeast in the sugarcane fermentation process for ethanol production. Nitrogen sources (N-sources) in the form of ammonium, nitrate, glutamate or glutamine were investigated with or without the addition of methionine sulfoximine, which inhibits the activity of the enzyme glutamine synthetase and releases cells from NCR...
June 19, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28612170/emended-description-of-the-family-chromatiaceae-phylogenetic-analyses-of-the-genera-alishewanella-rheinheimera-and-arsukibacterium-transfer-of-rheinheimera-longhuensis-lh2-2-t-to-the-genus-alishewanella-and-description-of-alishewanella-alkalitolerans-sp-nov
#18
Shivaji Sisinthy, Dwaipayan Chakraborty, Harikrishna Adicherla, Sathyanarayana Reddy Gundlapally
Phylogenetic analyses were performed for members of the family Chromatiaceae, signature nucleotides deduced and the genus Alishewanella transferred to Chromatiaceae. Phylogenetic analyses were executed for the genera Alishewanella, Arsukibacterium and Rheinheimera and the genus Rheinheimera is proposed to be split, with the creation of the Pararheinheimera gen. nov. Furthermore, the species Rheinheimera longhuensis, is transferred to the genus Alishewanella as Alishewanella longhuensis comb. nov. Besides, the genera Alishewanella and Rheinheimera are also emended...
June 13, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28608318/denitrifying-bacterial-communities-display-different-temporal-fluctuation-patterns-across-dutch-agricultural-soils
#19
Nguyen E López-Lozano, Michele C Pereira E Silva, Franck Poly, Nadine Guillaumaud, Jan Dirk van Elsas, Joana Falcão Salles
Considering the great agronomic and environmental importance of denitrification, the aim of the present study was to study the temporal and spatial factors controlling the abundance and activity of denitrifying bacterial communities in a range of eight agricultural soils over 2 years. Abundance was quantified by qPCR of the nirS, nirK and nosZ genes, and the potential denitrification enzyme activity (DEA) was estimated. Our data showed a significant temporal variation considerably high for the abundance of nirK-harboring communities, followed by nosZ and nirS communities...
June 12, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28608317/ica-status-of-clinical-staphylococcus-epidermidis-strains-affects-adhesion-and-aggregation-a-thermodynamic-analysis
#20
Titik Nuryastuti, Bastiaan P Krom
Staphylococcus epidermidis is a major nosocomial pathogen associated with infections of indwelling medical devices. One important virulence factor of these organisms is their ability to adhere to devices and form biofilms. In this study, we evaluated the effect of the ica operon on cell surface hydrophobicity, thermodynamics of adhesion, and biofilm formation for seven S. epidermidis strains. The surface free energy parameters of the bacterial cell surface and the substratum were determined by contact angle measurement...
June 12, 2017: Antonie Van Leeuwenhoek
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