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Antonie Van Leeuwenhoek

Zheng-Fei Yan, Pei Lin, Chang-Tian Li, MooChang Kook, Tae-Hoo Yi
A Gram-stain positive, aerobic, non-motile, endospore-forming and rod-shaped strain (THG-NT9T ) was isolated from a green tea sample. Growth occurred at 20-45 °C (optimum 28-35 °C), at pH 6.0-8.0 (optimum 7.0) and at 0-2.0% NaCl (optimum 0%). Based on 16S rRNA gene sequence analysis, the near phylogenetic neighbours of strain THG-NT9T were identified as Scopulibacillus daqui DSM 28236T (98.6%), Scopulibacillus darangshiensis DSM 19377T (97.4%), Pullulanibacillus pueri CGMCC 1.12777T (96.7%) and Pullulanibacillus camelliae CGMCC 1...
May 22, 2018: Antonie Van Leeuwenhoek
Josef D Franke, Wilson R Blomberg, Robert T Todd, Robert W Thomas, Anna M Selmecki
Gemmata obscuriglobus is a Gram-negative bacterium with several intriguing biological features. Here, we present a complete, de novo whole genome assembly for G. obscuriglobus which consists of a single, circular 9 Mb chromosome, with no plasmids detected. The genome was annotated using the NCBI Prokaryotic Genome Annotation pipeline to generate common gene annotations. Analysis of the rRNA genes revealed three interesting features for a bacterium. First, linked G. obscuriglobus rrn operons have a unique gene order, 23S-5S-16S, compared to typical prokaryotic rrn operons (16S-23S-5S)...
May 21, 2018: Antonie Van Leeuwenhoek
Joanna Mokracka, Sylwia Krzymińska, Danił Ałtunin, Dariusz Wasyl, Ryszard Koczura, Krzysztof Dudek, Monika Dudek, Zofia Anna Chyleńska, Anna Ekner-Grzyb
The aim of this study was to estimate virulence potential of Salmonella enterica strains colonizing the gut of free-living sand lizards (Lacerta agilis L.). The strains belonged to three Salmonella serovars: Abony, Schleissheim, and Telhashomer. Adhesion and invasion abilities of the strains were determined in quantitative assays using the gentamicin protection method. Induction of apoptosis was assessed using HeLa cell monolayers. PCR assays were used for detection of 26 virulence genes localised within mobile elements: pathogenicity islands, virulence plasmids, and prophage sequences...
May 19, 2018: Antonie Van Leeuwenhoek
Liying Jin, Junwei Zhao, Shanwen Jiang, Yang Zhao, Xiaoyang Han, Xiaowei Guo, Xiangjing Wang, Wensheng Xiang
Two Gram-stain positive, aerobic actinomycete strains, designated NEAU-JGR1T and NEAU-JGC41, were isolated from soil collected from Fairy Lake Botanical Garden in Shenzhen, Guangdong Province, south of China. The 16S rRNA gene sequences analysis showed that the two strains exhibited 99.5% 16S rRNA gene sequence similarity with each other and were closely related to Promicromonospora thailandica JCM 17130T (99.4, 99.3%) and Promicromonospora citrea DSM 43110T (99.2, 99.2%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains clustered together and formed a cluster with P...
May 19, 2018: Antonie Van Leeuwenhoek
Adriaana Jacobs, Lydia Mojela, Brett Summerell, Eduard Venter
The genus Fusarium hosts a large number of economically significant phytopathogens with a global distribution. Surprisingly, only a limited number of studies have tried to identify the natural distribution of members of this genus in undisturbed soils. Members of the Fusarium incarnatum-equiseti species complex (FIESC) are increasingly associated with plant disease, and human and animal health problems. Recently, an outbreak of kikuyu poisoning of cattle was attributed to the F. incarnatum-equiseti species complex...
May 18, 2018: Antonie Van Leeuwenhoek
Wei Jin, Yin Li, Yanfen Cheng, Shengyong Mao, Weiyun Zhu
The cecum plays an important role in the feed fermentation of ruminants. However, information is very limited regarding the cecal microbiota and their methane production. In the present study, the cecal content from twelve local Chinese goats, fed with either a hay diet (0% grain) or a high-grain diet (71.5% grain), were used to investigate the bacterial and archaeal community and their methanogenic potential. Microbial community analysis was determined using high-throughput sequencing of 16S rRNA genes and real-time PCR, and the methanogenesis potential was assessed by in vitro fermentation with ground corn or hay as substrates...
May 17, 2018: Antonie Van Leeuwenhoek
Salih Saricaoglu, Imen Nouioui, Hilal Ay, Hayrettin Saygin, Kadriye Inan Bektas, Kiymet Guven, Demet Cetin, Hans-Peter Klenk, Kamil Isik, Nevzat Sahin
Strain H2R21T , a novel actinobacterium, isolated from a forest soil sample collected from Heybeliada, Istanbul, Turkey, and a polyphasic approach was used for characterisation of the strain. Chemotaxonomic and morphological characterisation of strain H2R21T indicated that it belongs to the genus Nonomuraea. 16S rRNA gene sequence similarity showed that the strain is closely related to Nonomuraea purpurea 1SM4-01T (99.1%) and Nonomuraea solani CGMCC 4.7037T (98.4%). DNA-DNA relatedness values were found to be lower than 70% between the isolate and its phylogenetic neighbours N...
May 16, 2018: Antonie Van Leeuwenhoek
Cletus P Kurtzman, Christie J Robnett, Eleanor Basehoar, Todd J Ward
From comparisons of ITS1-5.8S-ITS2 and gene sequences for nuclear D1/D2 LSU rRNA, nuclear SSU (18S) rRNA, translation elongation factor 1-α (EF1-α) and RNA polymerase II subunit 2 (RPB2), the following four new ascosporogenous yeast species were resolved and are described as Metschnikowia anglica (NRRL Y-7298T [type strain], CBS 15342, MycoBank MB 823167), Metschnikowia leonuri (NRRL Y-6546T , CBS 15341, MB 823166), Metschnikowia peoriensis (NRRL Y-5942T , CBS 15345, MB 823164) and Metschnikowia rubicola (NRRL Y-6064T , CBS 15344, MB 823165)...
May 12, 2018: Antonie Van Leeuwenhoek
Lorena Escudero, Nia Oetiker, Karem Gallardo, Cinthya Tebes-Cayo, Mariela Guajardo, Claudia Nuñez, Carol Davis-Belmar, J J Pueyo, Guillermo Chong Díaz, Cecilia Demergasso
The endorheic basins of the Northern Chilean Altiplano contain saline lakes and salt flats. Two of the salt flats, Gorbea and Ignorado, have high acidic brines. The causes of the local acidity have been attributed to the occurrence of volcanic native sulfur, the release of sulfuric acid by oxidation, and the low buffering capacity of the rocks in the area. Understanding the microbial community composition and available energy in this pristine ecosystem is relevant in determining the origin of the acidity and in supporting the rationale of conservation policies...
May 10, 2018: Antonie Van Leeuwenhoek
Cristina Dorador, Patrick Fink, Martha Hengst, Gonzalo Icaza, Alvaro S Villalobos, Drina Vejar, Daniela Meneses, Vinko Zadjelovic, Lisa Burmann, Jana Moelzner, Chris Harrod
The geological, hydrological and microbiological features of the Salar de Atacama, the most extensive evaporitic sedimentary basin in the Atacama Desert of northern Chile, have been extensively studied. In contrast, relatively little attention has been paid to the composition and roles of microbial communities in hypersaline lakes which are a unique feature in the Salar. In the present study biochemical, chemical and molecular biological tools were used to determine the composition and roles of microbial communities in water, microbial mats and sediments along a marked salinity gradient in Laguna Puilar which is located in the "Los Flamencos" National Reserve...
May 9, 2018: Antonie Van Leeuwenhoek
Junwei Zhao, Shan Mu, Qiannan Zhao, Shanwen Jiang, Peng Cao, Xiaowei Guo, Xiangjing Wang, Wensheng Xiang
A novel actinomycete, designated strain NEAU-mq18T , was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell. Arg) collected from Xianglu Mountain in Heilongjiang Province, northeast China, and subjected to a polyphasic taxonomic study. The 16S rRNA gene sequence analysis showed that the isolate belongs to the genus Nonomuraea with high sequence similarity to Nonomuraea guangzhouensis NEAU-ZJ3T (98.5%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain clusters phylogenetically with N...
May 9, 2018: Antonie Van Leeuwenhoek
Michael Goodfellow, Imen Nouioui, Roy Sanderson, Feiyang Xie, Alan T Bull
An "in house" taxonomic approach to drug discovery led to the isolation of diverse actinobacteria from hyper-arid, extreme hyper-arid and very high altitude Atacama Desert soils. A high proportion of the isolates were assigned to novel taxa, with many showing activity in standard antimicrobial plug assays. The application of more advanced taxonomic and screening strategies showed that strains classified as novel species of Lentzea and Streptomyces synthesised new specialised metabolites thereby underpinning the premise that the extreme abiotic conditions in the Atacama Desert favour the development of a unique actinobacterial diversity which is the basis of novel chemistry...
May 2, 2018: Antonie Van Leeuwenhoek
Yan-Jiao Zhang, Huiyuan Lin, Pan Wang, Chang Chen, Shiyong Chen
Vibrio vulnificus, a Gram-negative halophilic bacterium, is an opportunistic human pathogen that is responsible for the majority of seafood-associated deaths worldwide. Lipoproteins are important components of the bacterial cell envelope and have been shown to be involved in a wide variety of cellular processes. Little is known about the localisation or transport mechanism of lipoproteins in V. vulnificus. To assess the localisation of lipoproteins in V. vulnificus, we tested two established techniques for the rapid separation of membrane-associated proteins: detergent extraction with Sarkosyl and outer membrane vesicles (OMVs) preparation...
May 2, 2018: Antonie Van Leeuwenhoek
Qing Chen, Hong-Mei Wang, Wen Zhuang, Zhong-Guan Sun, Jing Chen, Yan Ge, Ji-Guo Qiu, Jian He
Strain ZZ-8T , a Gram-negative, aerobic, non-spore-forming, non-motile, yellow-pigmented, rod-shaped bacterium, was isolated from metolachlor-contaminated soil in China. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ZZ-8T is a member of the genus Flavobacterium and shows high sequence similarity to Flavobacterium humicola UCM-46T (97.2%) and Flavobacterium pedocola UCM-R36T (97.1%), and lower (< 97%) sequence similarity to other known Flavobacterium species...
April 30, 2018: Antonie Van Leeuwenhoek
Olga Maria Lage, Damien P Devos
No abstract text is available yet for this article.
April 25, 2018: Antonie Van Leeuwenhoek
Viktoria Michel, Claudia Ulber, Dietrich Pöhle, Beate Köpke, Katharina Engel, Ute Kaim, Ahmad Fawzy, Sophie Funk, Juliane Fornefett, Christoph Georg Baums, Tobias Eisenberg
Rat bite fever is an under-reported, under-diagnosed emerging zoonosis with worldwide distribution. Besides Spirillum minus, Streptobacillus moniliformis is the major causative microorganism although it usually colonises rats without any clinical signs. A group of house rats (Rattus rattus) kept in a zoo exhibition for educational purposes suffered from neurological signs including disorientation, torticollis, stall walking, ataxia and death. Gross pathological and histo-pathological examinations of the investigated rats revealed high-grade otitis interna et media, from which Streptobacillus notomytis was isolated in pure culture or as the predominant microorganism...
April 18, 2018: Antonie Van Leeuwenhoek
Hyosun Lee, Dong-Uk Kim, Suyeon Lee, Song-Gun Kim, A-Young Park, Jae-Hyung Ahn, Jong-Ok Ka
A Gram-stain negative, aerobic, non-motile, rod-shaped and yellow bacterium, designated TX0651T , was isolated from an automotive air-conditioning system. Phylogenetically, the strain groups with the members of the genus Flavisolibacter and exhibits high 16S rRNA gene sequence similarities with Flavisolibacter ginsenosidimutans Gsoil 636T (97.4%), Flavisolibacter ginsengiterrae Gsoil 492T (96.3%) and Flavisolibacter ginsengisoli Gsoil 643T (96.2%). DNA-DNA relatedness between TX0651T and F. ginsenosidimutans KCTC 22818T and F...
April 18, 2018: Antonie Van Leeuwenhoek
Thalita Macedo Araújo, Magalhães Teixeira Souza, Raphael Hermano Santos Diniz, Celina Kiyomi Yamakawa, Lauren Bergmann Soares, Jaciane Lutz Lenczak, Juliana Velasco de Castro Oliveira, Gustavo Henrique Goldman, Edilene Alves Barbosa, Anna Clara Silva Campos, Ieso Miranda Castro, Rogelio Lopes Brandão
This work was performed to verify the potential of yeast strains isolated from cachaça distilleries for two specific biotechnological applications: beer and bioethanol production. In the beer production, the strains were tested for characteristics required in brewery practices, such as: capacity to ferment maltose and maltotriose, ability to grow at lowest temperatures, low H2 S production, and flocculation profile. Among the strains tested, two of them showed appropriate characteristics to produce two different beer styles: lager and ale...
April 16, 2018: Antonie Van Leeuwenhoek
Renan N Barbosa, Jadson D P Bezerra, Cristina M Souza-Motta, Jens C Frisvad, Robert A Samson, Neiva T Oliveira, Jos Houbraken
Penicillium and Talaromyces species have a worldwide distribution and are isolated from various materials and hosts, including insects and their substrates. The aim of this study was to characterize the Penicillium and Talaromyces species obtained during a survey of honey, pollen and the inside of nests of Melipona scutellaris. A total of 100 isolates were obtained during the survey and 82% of those strains belonged to Penicillium and 18% to Talaromyces. Identification of these isolates was performed based on phenotypic characters and β-tubulin and ITS sequencing...
April 13, 2018: Antonie Van Leeuwenhoek
Dong Kyung Lee, Tom Hsiang, Marc-André Lachance
Genes involved in mating type determination and recognition were examined in Metschnikowia and related species, to gather insights on factors affecting mating compatibility patterns among haplontic, heterothallic yeast species of the genus. We confirmed the universality of the special mating locus organisation found in Clavispora lusitaniae across and exclusive to the family Metschnikowiaceae (i.e., Metschnikowia and Clavispora). Timing of the divergence between idiomorphs was confirmed to coincide with the origin of the larger (CUG-ser) clade comprising the Debaryomycetaceae and the Metschnikowiaceae, exclusive of Cephaloascus fragrans...
April 12, 2018: Antonie Van Leeuwenhoek
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