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Antonie Van Leeuwenhoek

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https://www.readbyqxmd.com/read/28523377/mining-lipolytic-enzymes-in-community-dna-from-high-andean-soils-using-a-targeted-approach
#1
Daniel Borda-Molina, José Salvador Montaña, María Mercedes Zambrano, Sandra Baena
Microbial enrichments cultures are a useful strategy to speed up the search for enzymes that can be employed in industrial processes. Lipases have gained special attention because they show unique properties such as: broad substrate specificity, enantio- and regio-selectivity and stability in organic solvents. A major goal is to identify novel lipolytic enzymes from microorganisms living in cold extreme environments such as high Andean soils, of relevance to our study being their capability be used in industrial processes...
May 18, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28509971/description-of-chryseobacterium-timonianum-sp-nov-isolated-from-a-patient-with-pneumonia
#2
Rita Abou Abdallah, Teresa Cimmino, Sophie Baron, Frédéric Cadoret, Caroline Michelle, Didier Raoult, Pierre-Edouard Fournier, Fadi Bittar
Using a polyphasic taxonomic strategy, an aerobic, Gram-negative, non-motile, yellow pigmented rod isolated from a sputum sample of a patient with pneumonia was characterised. This bacterial strain, designated G972(T), could not be identified by our systematic MALDI-TOF screening on a MicroFlex. This led to the sequencing of the 16S rRNA gene, which shows 98.57% sequence identity with that of Chryseobacterium indologenes 16777(T), the phylogenetic closely related type strain of a species with standing in nomenclature, which putatively classifies it as a new species...
May 16, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28501914/skermanella-mucosa-sp-nov-isolated-from-crude-oil-contaminated-soil
#3
Y Subhash, Da-Eun Yoon, Sang-Seob Lee
A novel Gram-stain negative, small rod-shaped bacterium (strain 8-14-6(T)) was isolated from hydrocarbon contaminated desert soil collected from Kuwait. Strain 8-14-6(T) grew at 5-37 °C, pH 6.0-8.8 and 0-2% (w/v) of NaCl concentration. Casein, starch, Tween 20 and Tween 80 were hydrolyzed while urea, chitin, DNA and carboxymethyl-cellulose were not hydrolyzed by strain 8-14-6(T). The major cellular fatty acids were identified as C18:1ω6c/C18:1ω7c, C16:0 and iso-C16:1I/C14:03-OH. Strain 8-14-6(T) produced diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipids, two unidentified lipids and five unidentified amino lipids as polar lipids...
May 13, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28500545/nocardia-tengchongensis-sp-nov-isolated-from-a-soil-sample
#4
Shuai Li, Hong Ming, Yan-Yan Duan, Jian-Rong Huang, Zhuo-Li Zhao, Ling-Yu Zhang, Wei-Li Ji, Xiao-Lin Meng, Wen-Jun Li, Guo-Xing Nie
The taxonomic status was determined of two actinomycetes, designated CFH S0057(T) and CFH S0065, that were isolated from soil samples collected from an extinct volcano in Tengchong county, Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains CFH S0057(T) and CFH S0065 belong to the genus Nocardia and formed a single clade within this genus. The two isolates were able to grow at 4-45 °C, pH 5.0-7.0 and with a NaCl tolerance up to 5.0% (w/v). The whole-cell hydrolysates were rich in meso-diaminopimelic acid, galactose, arabinose and fructose...
May 12, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28500544/diversity-and-plant-growth-promoting-properties-of-rhizobia-isolated-from-root-nodules-of-ononis-arvensis
#5
Sylwia Wdowiak-Wróbel, Monika Marek-Kozaczuk, Michał Kalita, Magdalena Karaś, Magdalena Wójcik, Wanda Małek
This is the first report describing isolates from root nodules of Ononis arvensis (field restharrow). The aim of this investigation was to describe the diversity, phylogeny, and plant growth promoting features of microsymbionts of O. arvensis, i.e., a legume plant growing in different places of the southern part of Poland. Twenty-nine bacterial isolates were characterized in terms of their phenotypic properties, genome fingerprinting, and comparative analysis of their 16S rRNA, nodC and acdS gene sequences...
May 12, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28488064/rhodococcus-gannanensis-sp-nov-a-novel-endophytic-actinobacterium-isolated-from-root-of-sunflower-helianthus-annuus-l
#6
Jun Ma, Liguo Zhang, Guangjin Wang, Shuquan Zhang, Xiaofei Zhang, Yongbin Wang, Chuanqi Shi, Liang Si, Haibin Zhao, Fengqi Liu, Bixian Zhang, Xutang Huang
A novel Gram-positive, aerobic, non-motile strain, designated strain M1(T), was isolated from sunflower root (Helianthus annuus L.) and characterised using a polyphasic taxonomic approach. The morphological and chemotaxonomic properties of the isolate were typical of those of members of the genus Rhodococcus. Phylogenetic analyses based on 16S rRNA gene sequence showed that strain M1(T) belongs to the genus Rhodococcus and clustered with Rhodococcus canchipurensis MBRL 353(T) (99.1%, sequence similarity) and Rhodococcus pedocola UC12(T) (98...
May 9, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28477175/plipastatin-and-surfactin-coproduction-by-bacillus-subtilis-pb2-l-and-their-effects-on-microorganisms
#7
Ling Gao, Jinzhi Han, Hongxia Liu, Xiaoxu Qu, Zhaoxin Lu, Xiaomei Bie
To convert the lipopeptide non-producer strain Bacillus subtilis pB2 into a plipastatin and surfactin coproducer, a gene expression cassette composed of a constitutive promoter (P43), functional gene sfp, and pleiotropic regulatory gene degQ was integrated into the chromosomal amyE locus of strain B. subtilis pB2 by homologous recombination, which generated a plipastatin and surfactin co-producer. Thirteen plipastatins and fifteen surfactins were identified in lipopeptide extracts using analytical techniques, and their effects on microorganisms were described by microscopic, cytoskeleton analysis and flow-cytometry, respectively...
May 5, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28470566/altererythrobacter-lauratis-sp-nov-and-altererythrobacter-palmitatis-sp-nov-isolated-from-a-tibetan-hot-spring
#8
Chang-Guo Yuan, Xing Chen, Zhao Jiang, Wei Chen, Lan Liu, Wen-Dong Xian, Mipeshwaree Devi Asem, Xiao-Yang Zhi, Wen-Jun Li
Two Gram-negative, moderately thermophilic, yellow-pigmented, rod-shaped and motile bacterial strains, designated YIM 75003(T) and YIM 75004(T), were isolated from sediment samples collected from the Tagejia hot spring in Tibet, western China. The taxonomic affiliation of the two strains was investigated by a polyphasic approach. Pairwise comparison of the 16S rRNA gene sequences showed that strains YIM 75003(T) and YIM 75004(T) are closely related to Altererythrobacter buctense M0322(T) (97.2 and 97.1% respectively), while sharing 99...
May 3, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28470565/a-simple-scaled-down-system-to-mimic-the-industrial-production-of-first-generation-fuel-ethanol-in-brazil
#9
Vijayendran Raghavendran, Thalita Peixoto Basso, Juliana Bueno da Silva, Luiz Carlos Basso, Andreas Karoly Gombert
Although first-generation fuel ethanol is produced in Brazil from sugarcane-based raw materials with high efficiency, there is still little knowledge about the microbiology, the biochemistry and the molecular mechanisms prevalent in the non-aseptic fermentation environment. Learning-by-doing has hitherto been the strategy to improve the process so far, with further improvements requiring breakthrough technologies. Performing experiments at an industrial scale are often expensive, complicated to set up and difficult to reproduce...
May 3, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28456894/arenimonas-alkanexedens-sp-nov-isolated-from-a-frozen-soil-sample
#10
Jie Zhu, Hui-Min Wang, Qi Zhang, Wei-Wei Dong, De-Long Kong, Yan-Wei Wang, Jin-Long Song, Xu Jiang, Shu-Miao Zhao, Wei Zhang, Zhi-Yong Ruan
A novel facultatively anaerobic bacterium, designated strain LAM-WHM-D11(T), was isolated from a frozen soil sample of China. Cells of strain LAM-WHM-D11(T) were observed to be Gram-stain negative, non-motile and rod-shaped. Colonies were yellowish, and circular with convex shape. Strain LAM-WHM-D11(T) was found to be able to grow at 4-40 °C (optimum 15 °C), pH 7.5-2.0 (optimum 9.5) and 0-2.5% NaCl (w/v) (optimum 1.5%). The 16S rRNA gene sequence similarity analysis showed that strain LAM-WHM-D11(T) is closely related to Arenimonas metalli CF5-1(T) (98...
April 29, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28455763/terrimonas-suqianensis-sp-nov-isolated-from-a-tetrabromobisphenol-a-contaminated-soil
#11
Qing Chen, Xiao-Xia Zang, Xing Hang, Jing Chen, Hong-Mei Wang, Shun-Peng Li, Jian-Dong Jiang, Kai Chen
Strain C3-5(T), a Gram-negative, asporogenous, rod-shaped bacterium, was isolated from a tetrabromobisphenol A contaminated soil. Growth was observed at 10-37 °C (optimum 30 °C) and at pH 5.5-9.5 (optimum pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain C3-5(T) is a member of the genus Terrimonas and exhibits high sequence similarities with Terrimonas pekingensis QH(T) (99.0%) and Terrimonas rhizosphaerae CR94(T) (97.3%), and exhibits low (<97%) sequence similarities with other known Terrimonas species...
April 28, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28455762/expression-of-the-stress-response-regulators-ctsr-and-hrca-in-the-uropathogen-staphylococcus-saprophyticus-during-heat-shock
#12
Ciro César Rossi, Lorayne Lauria de Oliveira, Deivid de Carvalho Rodrigues, Turán Peter Ürményi, Marinella Silva Laport, Marcia Giambiagi-deMarval
The uropathogen Staphylococcus saprophyticus is an ubiquitous bacterium but little is known about mechanisms that allow its persistence in diverse environments. Here we evaluated S. saprophyticus growth and survival during heat shock, the expression of stress response regulators ctsR and hrcA through qRT-PCR and heat shock protein synthesis through (35)S-Met metabolic labeling. S. saprophyticus does not tolerate temperatures much higher than the optimal 37 °C, as its growth is greatly affected at 42 °C, though viability is maintained up to 48 °C...
April 28, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28451868/juglone-alleviates-pneumolysin-induced-human-alveolar-epithelial-cell-injury-via-inhibiting-the-hemolytic-activity-of-pneumolysin
#13
Meng Song, Gejin Lu, Meng Li, Xuming Deng, Jianfeng Wang
Streptococcus pneumoniae (the pneumococcus) is an opportunistic pathogen responsible for several human diseases, including acute otitis media, pneumonia, sepsis and bacterial meningitis, and possesses numerous virulence factors associated with pneumococcal infection and pathogenesis. With the capacity to form pores in cholesterol-rich membranes, pneumolysin (PLY) is a key virulence factor of S. pneumoniae and causes severe tissue damage during pneumococcal infection. Juglone (JG), a natural 1,4-naphthoquinone widely found in the roots, leaves, woods and fruits of Juglandaceae walnut trees, inhibits PLY-induced hemolysis via inhibition of the oligomerization of PLY and exhibits minimal anti-S...
April 27, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28439679/screening-lactic-acid-bacteria-strains-with-ability-to-bind-di-n-butyl-phthalate-via-turbiscan-technique
#14
Zhao Lili, Zhao Hongfei, Sana Shoukat, Zhang Xiaochen, Zhang Bolin
Di-n-butyl phthalate (DBP) is a ubiquitous environmental contaminant that poses a risk to humans. Previous work indicates that the ability of lactic acid bacteria (LAB) to bind phthalic acid esters is strain-specific. As cell suspensions of LAB strains in aqueous solution are likely to be colloidal dispersions, this study provided a technique to efficiently screen LAB strains that bind DBP via Turbiscan, which has been widely used to measure the stability of emulsions or colloidal dispersions. Eleven LAB strains belonging to Lactobacillus plantarum, Lb...
April 24, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28439678/aquimarina-seongsanensis-sp-nov-isolated-from-sea-water
#15
Yoojin Oh, Bong-Geun Choi, Joon Yong Kim, Seong Woon Roh, Sung-Jae Lee
An orange, rod-shaped, gliding bacterium, designated strain CBA3208(T), was isolated from sea water of Jeju Island, Republic of Korea. Cells were observed to be Gram-stain negative, strictly aerobic, catalase positive and oxidase negative and to hydrolyse starch, gelatin, and Tweens 40 and 80. The major fatty acids were identified as iso-C15:0, iso-C17:0 3-OH, and iso-C15:1 G. The only isoprenoid quinone was found to be menaquinone-6 (MK-6) and the major polar lipids were identified as phosphatidylethanolamine, two aminolipids and four unidentified polar lipids...
April 24, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28434178/massilia-chloroacetimidivorans-sp-nov-a-chloroacetamide-herbicide-degrading-bacterium-isolated-from-soil
#16
Hyosun Lee, Dong-Uk Kim, Sooyeon Park, Jung-Hoon Yoon, Jong-Ok Ka
A Gram-stain negative, rod-shaped, aerobic bacterial strain, designated TA-C7e(T), was isolated from an agricultural soil obtained from Taean region, Korea. Phylogenetically, the strain was grouped with the genus Massilia and shared 95.33-97.97% 16S rRNA sequence similarity with the type strains of other species of the genus Massilia. The DNA-DNA hybridisation between TA-C7e(T) and Massilia haematophila KACC 13771(T), Massilia suwonesis KACC 12635(T), and Massilia yuzhufengensis KACC 16569(T) revealed a low level of relatedness (<40%)...
April 22, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28409238/studies-on-lipid-a-isolated-from-phyllobacterium-trifolii-petp02-t-lipopolysaccharide
#17
Katarzyna Zamlynska, Iwona Komaniecka, Kamil Zebracki, Andrzej Mazur, Anna Sroka-Bartnicka, Adam Choma
The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02(T), a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-D-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, substituted by galacturonic acid (GalpA) at position C-1 and partly decorated by a phosphate residue at C-4' of the non-reducing GlcpN3N. Both diaminosugars were symmetrically substituted by 3-hydroxy fatty acids (14:0(3-OH) and 16:0(3-OH))...
April 13, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28409237/prevalence-of-sucretolerant-bacteria-in-common-soils-and-their-isolation-and-characterization
#18
Casper Fredsgaard, Donald B Moore, Fei Chen, Benton C Clark, Mark A Schneegurt
Sucretolerant microbes grow in the presence of sugar concentrations high enough to substantially lower water activities. Natural habitats high in sugars are mainly limited to dried fruit, floral nectar, honey, sugarcane, and associated soils. Organisms that tolerate extremes of solute concentration, high enough to lower water activities, might not be expected in common oligoosmotic soils. We report on the isolation of sucretolerant bacteria from common soils using media supplemented with 50% sucrose (a w 0...
April 13, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28401363/characterization-of-marinovum-faecis-sp-nov-an-alphaproteobacterium-isolated-from-marine-sediment
#19
Jaewoo Yoon, So Young Kim, Songhee Park, Hyukjae Choi
A Gram-negative, strictly aerobic, chemoheterotrophic, beige-pigmented, ovoid bacterium, designated YP194(T), was isolated from marine sediment in the Republic of Korea. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain belongs to the family Rhodobacteraceae of the class Alphaproteobacteria, with high sequence similarity (98.4%) to Marinovum algicola FF3(T). The DNA-DNA relatedness values between strains YP194(T) and M. algicola FF3(T) were 34.1 ± 2.7%. The DNA G+C content of strain YP194(T) was 63...
April 11, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28393289/practically-delineating-bacterial-species-with-genealogical-concordance
#20
Stephanus N Venter, Marike Palmer, Chrizelle W Beukes, Wai-Yin Chan, Giyoon Shin, Elritha van Zyl, Tarren Seale, Teresa A Coutinho, Emma T Steenkamp
Bacterial species are commonly defined by applying a set of predetermined criteria, including DNA-DNA hybridization values, 16S rRNA gene sequence similarity, phenotypic data as well as genome-based criteria such as average nucleotide identity or digital DNA-DNA hybridization. These criteria mostly allow for the delimitation of taxa that resemble typical bacterial species. Their application is often complicated when the objective is to delineate new species that are characterized by significant population-level diversity or recent speciation...
April 9, 2017: Antonie Van Leeuwenhoek
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