Preparation of an Efficient Ratiometric Fluorescent Nanoprobe (m-CDs@[Ru(bpy) 3 ] 2+ ) for Visual and Specific Detection of Hypochlorite on Site and in Living Cells.

Hypochlorite (ClO- ) is one of the most important reactive oxygen species (ROS), which plays an important role in sustaining human innate immunity during microbial invasion. Moreover, ClO- is a powerful oxidizer for water treatment. The safety of drinking water is closely related to its content. Herein, m-phenylenediamine (mPD) is used as a precursor to prepare carbon dots (named m-CDs) with highly fluorescent quantum yield (31.58% in water), and our investigation shows that the strong fluorescent emission of m-CDs can be effectively quenched by ClO- . Based on these findings, we developed a novel fluorescent nanoprobe (m-CDs) for highly selective detection of ClO- . The linear range was from 0.05 to 7 μM (R2 = 0.998), and the limit of detection (S/N = 3) was as low as 0.012 μM. Moreover, a portable agarose hydrogel solid matrix-based ratiometric fluorescent nanoprobe (m-CDs@[Ru(bpy)3 ]2+ ) sensor was subsequently developed for visual on-site detection of ClO- with the naked eyes under a UV lamp, suggesting its potential in practical application with low cost and excellent performance in water quality monitoring. Additionally, intracellular detection of exogenous ClO- was demonstrated via ratiometric imaging microscopy.